CN102943043A

CN102943043A - High-efficiency agricultural microecological compound bacterium agent

Info

Publication number: CN102943043A
Application number: CN2012102719246A
Authority: CN
Inventors: 李峰
Original assignee: Individual
Current assignee: Individual
Priority date: 2012-08-02
Filing date: 2012-08-02
Publication date: 2013-02-27

Abstract

The invention discloses a high-efficiency agricultural microecological compound bacterium agent and belongs to the technical field of laboratory and factory fermentation tank culture of microbes. The high-efficiency agricultural microecological compound bacterium agent is obtained by mixing 30 to 45% by volume of a lactobacillus plantarum fermentation bacterium liquid, 35 to 45% by volume of a bacillus fermentation bacterium liquid and 15 to 30% of by volume of an actinomycete fermentation bacterium liquid. Lactobacillus plantarum, bacilli and actinomycetes are subjected to three-stage enlarge cultivation under the conditions of an appropriate temperature, appropriate oxygen content, appropriate nutrients and an appropriate pH for a certain cultivation time, wherein the three-stage enlarge cultivation is carried out in a strict aseptic environment. Through a modern biological engineering technology, a single type of bacteria obtained by high-concentration pure culture are compounded and the compounded bacteria are used for production of a high-efficiency microbial fertilizer.

Description

A kind of efficient agricultural micro-ecological complex bacterial agent

Technical field

The present invention relates to a kind of efficient agricultural micro-ecological complex bacterial agent, belong to laboratory and the factory's fermentation culture technology of bacterium

Background technology

The development of volume production agricultural makes a large amount of use of chemical fertilizer, agricultural chemicals and other chemical agricultural fertilizer, soil compaction, various direct output and the quality that affects agricultural-food of disease have been caused, major cause is that agriculture three-dimensional structure has been ignored in the development of modern agriculture, i.e. the circulation pattern of microorganism, plant and animal.Want to change this Agriculture Present Situation, must add beneficial microbe colony to planting soil, recover the microecological balance of soil.Agricultural microorganism microbial inoculum in the market is mainly single microbial strains, is difficult to reduce little ecologic structure, a little less than the synergy of common bacterial classification and farm crop, is unfavorable for improving stress resistance of plant and root system nutrition and absorbs simultaneously.

Summary of the invention

The present invention describes a kind of efficient agricultural micro-ecological complex bacterial agent, by composite with carrying out after the pure culture of individual plant microorganism high-density, form high functional flora and can play reduction soil microorganisms balance and promote farm crop to the effect of fertilizer and moisture absorption; Its high efficiency of efficient agricultural microbiobacterial agent is mainly embodied by the kind of its flora and the quantity of every kind of microorganism; Bacterium number after composite is 1,500,000,000; Article of the present invention this agricultural microorganism microbial inoculum the flora type and reach the cultural method of high bacterium number.

Technical scheme of the present invention is as follows: the efficient agricultural microecological microbial agent is to be mixed by milk-acid bacteria, genus bacillus and actinomycetic fermented liquid; The volume percent of each strain bacterium is: plant lactobacillus 30 ~ 45%, genus bacillus 35 ~ 45%, actinomycetes 15 ~ 30%.Described milk-acid bacteria is plant lactobacillus, and genus bacillus is subtilis, and actinomycetes are the Jingyang streptomycete.

The preparation method of above-mentioned efficient agricultural microecological microbial agent is that each bacterial classification is carried out respectively compound method preparation after fermentor tank middle-high density single culture, and the cultivation of every strain bacterium is to be cultivated by three grades of methods that enlarge gradually; The method comprises the steps:

(1) respectively milk-acid bacteria, genus bacillus and actinomycetic slant strains are inoculated in one-level shake-flask culture base and carry out the one-level cultivation, obtain first class inoculum;

(2) respectively cultured milk-acid bacteria, genus bacillus and actinomycetic one-level shaking flask bacterial classification inoculation are carried out secondary in the seed fermentation tank and cultivate, obtain secondary seed;

(3) respectively above-mentioned secondary seed is transferred to the second order fermentation tank and carries out three grades of cultivation acquisitions milk-acid bacteria, genus bacillus and actinomycetic fermented liquids;

(4) with each zymocyte liquid mixing in proportion.

The culture condition of above-mentioned each strain bacterium is respectively:

The bacillus subtilis bacterium culture medium is starch 0.5%, sucrose 0.7%, bean powder 2.5%, dipotassium hydrogen phosphate 0.35%, manganous sulfate 0.2%, yeast extract paste 0.2%, and inoculum size is 5% ~ 10%, and is preferred 10%, and cultivating pH is 6 ~ 8, and culture temperature is 30 ~ 35 ℃, and incubation time is 2 days; The plant lactobacillus substratum is yeast extract paste 1.5%, peptone 2.5%, glucose 2.5%, and inoculum size is 10%, and cultivating pH is 5.5, and culture temperature is 28 ~ 30 ℃, and incubation time is 2 days; The actinomycetes substratum is soybean cake powder 2.5%, CaCO ₃0.2%, NaCl0.3%, white sugar 1.5%, inoculum size is 5% ~ 10%, and is preferred 10%, and cultivating pH is 7.2, and culture temperature is 27 ~ 32 ℃, and incubation time is 4 days.

Beneficial effect of the present invention is: use modern microbial engineering, behind high-density pure culture individual plant microorganism, carry out composite, produce efficient microecological microbial agent, the three strain microorganisms that contain in the product can recover the edatope of ecosystem, balance soil micro-ecosystem and promotion plant absorb the equilibrium of nutrient, can the secondary pollution planting soil, higher than product efficacy on the market, quality is more stable.

Claims

1. an efficient agricultural complex microbial inoculum is characterized in that, it is to be cooperated by Bacterium lacticum, genus bacillus and actinomycetic pure growth to form; The volume ratio of each pure growth is: plant lactobacillus 30 ~ 45%, genus bacillus 35 ~ 45%, actinomycetes 15 ~ 30%.

2. efficient agricultural complex microbial inoculum according to claim 1 is characterized in that, described Bacterium lacticum plant lactobacillus; Described sporeformer is subtilis; Described actinomycetes are preferably the Jingyang streptomycete.

3. the preparation method of claim 1 or 2 described efficient agricultural complex microbial inoculums adopts each strain bacterium is carried out composite method preparation after the pure culture in the fermentation equipment after sterilization respectively, and the pure culture process of every strain bacterium is all by the method breeding of three grades of amplifications.

4. the breeding culture condition of the complex microbial inoculum of efficient agricultural described in the claim 3 is respectively: the bacillus subtilis bacterium culture medium is starch 0.5%, sucrose 0.7%, bean powder 2.5%, dipotassium hydrogen phosphate 0.35%, manganous sulfate 0.2%, yeast extract paste 0.2%, inoculum size is 5% ~ 10%, preferred 10%, cultivating pH is 6 ~ 8, culture temperature is 30 ~ 35 ℃, and incubation time is 2 days; The plant lactobacillus substratum is yeast extract paste 1.5%, peptone 2.5%, glucose 2.5%, and inoculum size is 10%, and cultivating pH is 5.5, and culture temperature is 28 ~ 30 ℃, and incubation time is 2 days; The actinomycetes substratum is soybean cake powder 2.5%, CaCO ₃0.2%, NaCl0.3%, white sugar 1.5%, inoculum size is 5% ~ 10%, and is preferred 10%, and cultivating pH is 7.2, and culture temperature is 27 ~ 32 ℃, and incubation time is 4 days.