CN102942549B - Method for extracting blueberry anthocyanin - Google Patents
Method for extracting blueberry anthocyanin Download PDFInfo
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- CN102942549B CN102942549B CN201210487338.5A CN201210487338A CN102942549B CN 102942549 B CN102942549 B CN 102942549B CN 201210487338 A CN201210487338 A CN 201210487338A CN 102942549 B CN102942549 B CN 102942549B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/54—Improvements relating to the production of bulk chemicals using solvents, e.g. supercritical solvents or ionic liquids
Abstract
The invention discloses a method for extracting blueberry anthocyanin with good stability. Raw materials are crushed into fine powder in a range of 20 meshes to 60 meshes; ethanol water of 60% is used for extraction for three times, wherein the extraction temperature is 50 DEG C, and the extraction time is 2h, 2h and 1h respectively; vacuum concentration is performed to recycle ethanol, pure water is added in a concentrate for precipitation and impurity removing, and a supernate is subjected to membrane separation and secondary vacuum concentration; supercritical CO2 extraction separation is performed, wherein the extraction temperature is 35 DEG C, and the pressure is 25 MPa; and vacuum drying is performed after refining and impurity removing, dry pastes are crushed and screened, accessories are added, and the mixture is mixed evenly, inspected, packaged and put in storage. Low-temperature separation techniques of membrane separation techniques, supercritical extraction separation techniques and the like are used to extract superior blueberry extract powder. The blueberry anthocyanin is not easy to change colors and is good in stability.
Description
Technical field
The present invention relates to a kind of extracting method of anthocyanidin, particularly a kind of extracting method of vaccinium oxycoccus pigment of good stability.
Background technology
Blueberry, has another name called blueberry, blue berry, HONGDOUGUO etc., is Ericaceae genus vaccinium plant, in shrub, is distributed in subfrigid zone, temperate zone and subtropics.Containing abundant nutritive ingredient in blue berry, except containing except the organic acid in general fruit, VITAMIN and mineral substance, also containing abundant unsaturated fatty acids and trace element, the multiple phenols physiologically active ingredient such as anthocyanidin, flavones, there is extremely strong pharmaceutical use and trophic function, be classified as one of five large heath food by national food and agricultural organization.Rapidly, market capacity is large in development in recent years.See according to the long term growth of industry, increase blueberry preliminary working and deep processed product, lengthening manufacturing chain bar, promotes its added value of product, imperative.
Anthocyanidin, also known as cyanidin(e), is the water-soluble natural pigment that a class is extensively present in plant, and belong to flavonoid compound, itself and glucide are anthocyanogen after being combined with glycosidic link, are a kind of natural antioxidants.Because cyanidin(e) is unstable, mainly exist with anthocyanogen in plant.
Anthocyanidin is as a kind of natural food colour, and safe, nontoxic, aboundresources, and have certain nutrition and pharmacological action, has huge application potential in food, cosmetic, medicine etc.Anthocyanidin is the antidotal nutritious supplementary of pure natural, and research proves mankind nowadays and finds most good antioxidant, is the most effective antioxidant of current nature.There is research title can vision enhancing, anti-eye strain simultaneously; Delay cranial nerve aging.
And anthocyanidin contained by blueberry is that in current all Anthocyanins, function is the most excellent, range of application is the widest, and side effect is minimum, is also the kind that price is the most expensive.The blueberry extract price that anthocyanidin content reaches 25% is that content reaches the 5-6 of the Semen Vitis viniferae extract of 95% doubly, its value free from worldly cares as seen.All containing anthocyanidin in blueberry root, stem, leaf, pericarp, pulp, and be present in the vacuole of blueberry cell.
The extraction process of existing vaccinium oxycoccus pigment is: raw material-pulverizing-alcohol extracting-depositing in water-filtration-mistake post absorption-wash-out-concentrated-drying-pulverizing-packaging-finished product, its stability is good not, comparatively fugitive color, makes troubles to daily production application aspect.
Summary of the invention
For prior art Problems existing, it is not easy to change that the present invention aims to provide one, and the extracting method of the vaccinium oxycoccus pigment of good stability.
The invention provides a kind of extracting method of vaccinium oxycoccus pigment of good stability, comprise the steps:
S1, to get the raw materials ready: raw material powder is broken into 20-60 object fine powder;
S2, alcohol extracting: the ethanol-water extraction with 60% three times, Extracting temperature is 50 DEG C, and extraction time is respectively 2h, 2h, 1h;
S3, depositing in water: after vacuum concentration reclaims ethanol, enriched material is added the impurity elimination of pure water precipitation;
S4, membrane sepn: by supernatant liquor vacuum concentration again after membrane sepn;
S5, supercritical fluid extraction and fractionation: the enriched material SCF-CO 2 of secondary concentration is separated, and extraction temperature is 35 DEG C, pressure is 25MPa;
S6, refining: refining impurity elimination final vacuum is dry further, and sieves broken for dried cream powder;
S7: batching: add auxiliary material and mix rear inspection, packaging, warehouse-in.
Preferably, during vacuum concentration, its vacuum tightness is-0.07MPa ~-0.088MPa.
Preferably, its temperature < 40 DEG C, pressure < 0.1MPa during membrane sepn.
Preferably, overcritical separating and extracting thing time-division I temperature is 45 DEG C, and pressure is 12MPa; Divide II temperature to be 35 DEG C, pressure is 6MPa.
Preferably, during vacuum-drying, its vacuum tightness is-0.07MPa ~-0.088MPa, temperature < 70 DEG C.
Preferably, auxiliary material described in batching step is foodstuff additive.
The extracting method of the vaccinium oxycoccus pigment of described good stability provided by the invention utilizes the low ternperature separation process technology such as membrane separation technique and supercritical fluid extraction and fractionation technology can extract the blueberry extract powder obtaining high-quality, not easy to change, and good stability.
Embodiment
Technical scheme of the present invention is further illustrated below by embodiment:
The invention provides a kind of extracting method of vaccinium oxycoccus pigment of good stability, comprise the steps:
S1, to get the raw materials ready: raw material powder is broken into 20-60 object fine powder;
S2, alcohol extracting: the ethanol-water extraction with 60% three times, Extracting temperature is 50 DEG C, and extraction time is respectively 2h, 2h, 1h;
S3, depositing in water: after vacuum concentration reclaims ethanol, enriched material is added the impurity elimination of pure water precipitation, during vacuum concentration, vacuum tightness is-0.07MPa ~-0.088MPa;
S4, membrane sepn: by supernatant liquor vacuum concentration again after membrane sepn, its temperature < 40 DEG C, pressure < 0.1MPa during membrane sepn, during vacuum concentration, its vacuum tightness is-0.07MPa ~-0.088MPa;
S5, supercritical fluid extraction and fractionation: the enriched material SCF-CO 2 of secondary concentration is separated, and extraction temperature is 35 DEG C, pressure is 25MPa, and wherein divide I temperature to be 45 DEG C, pressure is 12MPa; Divide II temperature to be 35 DEG C, pressure is 6MPa;
S6, refining: refining impurity elimination final vacuum is dry further, and sieves broken for dried cream powder, and wherein during vacuum-drying, its vacuum tightness is-0.07MPa ~-0.088MPa, temperature < 70 DEG C;
S7: batching: add auxiliary material and mix rear inspection, packaging, warehouse-in, described auxiliary material is specially foodstuff additive.
The extracting method of the vaccinium oxycoccus pigment of described good stability provided by the invention utilizes the low ternperature separation process technology such as membrane separation technique and supercritical fluid extraction and fractionation technology can extract the blueberry extract powder obtaining high-quality, not easy to change, and good stability.
Above to invention has been exemplary description; obvious realization of the present invention is not subject to the restrictions described above; as long as have employed the various improvement that technical solution of the present invention is carried out; or design of the present invention and technical scheme directly applied to other occasion, all in protection scope of the present invention without to improve.
Claims (2)
1. an extracting method for vaccinium oxycoccus pigment, is characterized in that, comprises the steps:
S1, to get the raw materials ready: raw material powder is broken into 20-60 object fine powder;
S2, alcohol extracting: the ethanol-water extraction with 60% three times, Extracting temperature is 50 DEG C, and extraction time is respectively 2h, 2h, 1h;
S3, depositing in water: after vacuum concentration reclaims ethanol, enriched material is added the impurity elimination of pure water precipitation; During vacuum concentration, its vacuum tightness is-0.07MPa ~-0.088MPa;
S4, membrane sepn: by supernatant liquor vacuum concentration again after membrane sepn; Temperature < 40 DEG C, pressure < 0.1MPa during membrane sepn;
S5, supercritical fluid extraction and fractionation: by the enriched material supercritical CO of secondary concentration
2extracting and separating, and extraction temperature is 35 DEG C, pressure is 25MPa;
Overcritical separating and extracting thing time-division I temperature is 45 DEG C, and pressure is 12MPa; Divide II temperature to be 35 DEG C, pressure is 6MPa;
S6, refining: refining impurity elimination final vacuum is dry further, and sieves broken for dried cream powder; During vacuum-drying, its vacuum tightness is-0.07MPa ~-0.088MPa, temperature < 70 DEG C;
S7: batching: add auxiliary material and mix rear inspection, packaging, warehouse-in.
2. the extracting method of vaccinium oxycoccus pigment according to claim 1, is characterized in that, auxiliary material described in batching step is foodstuff additive.
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| CN201210487338.5A CN102942549B (en) | 2012-11-27 | 2012-11-27 | Method for extracting blueberry anthocyanin |
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| CN201210487338.5A CN102942549B (en) | 2012-11-27 | 2012-11-27 | Method for extracting blueberry anthocyanin |
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| CN102942549A CN102942549A (en) | 2013-02-27 |
| CN102942549B true CN102942549B (en) | 2015-06-03 |
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106214729A (en) * | 2016-07-28 | 2016-12-14 | 南京正宽医药科技有限公司 | A kind of blue berry extracting method and the bathomorphic health tea for the treatment of |
| CN106380458A (en) * | 2016-08-30 | 2017-02-08 | 福建光泽圣祥源酒业有限公司 | Method for extracting anthocyanidin in blueberry |
| CN106539086A (en) * | 2016-11-30 | 2017-03-29 | 贵阳学院 | It is a kind of to improve blueberry anthocyanin oral solution of vision and preparation method thereof |
| CN107759555A (en) * | 2017-10-30 | 2018-03-06 | 潍坊友容实业有限公司 | A kind of Suaeda salsa anthocyanidin extracting method |
| CN107987048B (en) * | 2017-12-15 | 2020-05-05 | 齐鲁工业大学 | Technical method for extracting blueberry fruit anthocyanin by supercritical carbon dioxide based on infrared impact drying pretreatment |
| CN109761946A (en) * | 2019-03-05 | 2019-05-17 | 湖南华康生物科技股份有限公司 | A kind of extraction separation method of Cranberry anthocyanidin |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1634915A (en) * | 2004-11-15 | 2005-07-06 | 西安景行科技有限公司 | Method for extracting anthocyanidin |
| US20060280816A1 (en) * | 2000-08-31 | 2006-12-14 | Phenolics, Llc | Efficient method for producing compositions enriched in total phenols |
| CN101732483A (en) * | 2010-01-27 | 2010-06-16 | 吉林春源生物科技有限公司 | Health product manufactured by using extracts of Changbai mountain wild blueberry as raw material |
| CN102321062A (en) * | 2011-06-08 | 2012-01-18 | 吉林市新科奇保健食品有限公司 | Separating, purifying and inspecting method of anthocyanin in blueberry wine dregs |
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- 2012-11-27 CN CN201210487338.5A patent/CN102942549B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060280816A1 (en) * | 2000-08-31 | 2006-12-14 | Phenolics, Llc | Efficient method for producing compositions enriched in total phenols |
| CN1634915A (en) * | 2004-11-15 | 2005-07-06 | 西安景行科技有限公司 | Method for extracting anthocyanidin |
| CN101732483A (en) * | 2010-01-27 | 2010-06-16 | 吉林春源生物科技有限公司 | Health product manufactured by using extracts of Changbai mountain wild blueberry as raw material |
| CN102321062A (en) * | 2011-06-08 | 2012-01-18 | 吉林市新科奇保健食品有限公司 | Separating, purifying and inspecting method of anthocyanin in blueberry wine dregs |
Non-Patent Citations (1)
| Title |
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| 蓝莓果中花青素的乙醇提取工艺研究;马养民等;《安徽农业科学》;20111231;第39卷(第35期);第21768-21769页，第21958页 * |
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Effective date of registration: 20161028 Address after: 410000 Hunan province Changsha Wenxuan High-tech Development Zone, Road No. 27 Lu Valley Yuyuan F3 building N unit 10 storey 1002 room Patentee after: Hunan Huakang biological Polytron Technologies Inc Address before: 410000 Hunan province Changsha Yuhua District Environmental Protection Changsha Road No. 188 International Business Center Building 2 room D103 Patentee before: Hagen Biotechnology Co., Ltd. |