CN102924617A - Chinese wolfberry extract and preparation method thereof - Google Patents
Chinese wolfberry extract and preparation method thereof Download PDFInfo
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- CN102924617A CN102924617A CN2012104080769A CN201210408076A CN102924617A CN 102924617 A CN102924617 A CN 102924617A CN 2012104080769 A CN2012104080769 A CN 2012104080769A CN 201210408076 A CN201210408076 A CN 201210408076A CN 102924617 A CN102924617 A CN 102924617A
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Abstract
The invention discloses a Chinese wolfberry extract. 50 to 95 weight percent of polysaccharide is contained in the extract. The invention also discloses a preparation method for the Chinese wolfberry extract. The method comprises the following steps of: (a), adding water into cleaned Chinese wolfberry fruits which are taken as a raw material, and crushing a Chinese wolfberry and water mixture to obtain solid mixture slurry; (b), concentrating the solid mixture slurry by an ultrafilter to obtain a concentrated solution, adding ethanol at the volume concentration of 70 to 80 percent into the concentrated solution, standing, adding water to completely dissolve a precipitate, filtering, adding ethanol, standing a precipitate, and drying to obtain a dry substance; and (c), completely dissolving the dry substance by using water, ultrafiltering by an ultrafiltration device, concentrating a solution, adsorbing a concentrated solution on nonpolar macroporous resin, eluting, drying, and concentrating to obtain the Chinese wolfberry extract. In lycium barbarum polysaccharide, alkaline polysaccharide which has a low medicine effect is removed, and effective acid polysaccharose and neutral polysaccharide are reserved; and the ultrafiltration and toluene elution are adopted, so that the extraction rate is high.
Description
Technical field
The present invention relates to a kind of wolfberry fruit extract.
The invention still further relates to a kind of preparation method of wolfberry fruit extract.
Background technology
The effective constituent of wolfberry fruit extract is lycium barbarum polysaccharide, a kind of immunomodulator, energy immune cell activated, the immunologic function of raising body, in the face of normal cell does not have toxic side effect, also have reducing blood-fat, anti-inflammatory, many-sided activity such as antiviral, existing document has been put down in writing the extraction Isolation and purification method of polysaccharide, mostly adopt, the method of column chromatography, this method can't be produced on a large scale, causes the wasting of resources.Therefore, need to provide a kind of new technical scheme to solve the problems referred to above.
Summary of the invention
The purpose of this invention is to provide a kind of wolfberry fruit extract.
Another object of the present invention provides a kind of preparation method of wolfberry fruit extract, and this preparation method's extraction yield is high, and reactions steps is simple.
The technical solution used in the present invention:
Wolfberry fruit extract, the weight percent content of polysaccharide is 50-95% in the described extract.
The weight percent content of polysaccharide is 90-95% in the described extract.
The preparation method of wolfberry fruit extract may further comprise the steps:
A, take the Chinese wolfberry fruit cleaned as raw material, add water, the weight that adds water be matrimony vine weight 10-20 doubly, then the mixture of matrimony vine and water is pulverized, get the solid mixture slurries;
B, above-mentioned solid mixture slurries concentrate with ultrafilter, get concentrated solution, add the ethanol of volumetric concentration 40-60% in the concentrated solution, leave standstill, throw out is dissolved in water fully, filters, and filtrate adds the ethanol of volumetric concentration 70-80%, staticly settle thing, drying gets dry thing;
C, get dry thing, the water dissolve complete, carry out ultrafiltration with ultra-filtration equipment, its molecular retention amount is 10000, keeps the solution greater than molecular weight 10000, and solution is concentrated, concentrated solution is adsorbed on the non-polar macroporous resin, with the toluene wash-out of volumetric concentration 60-70%, reclaim the toluene elutriant, obtain wolfberry fruit extract after dry, concentrated.
Ultrafilter concentrates in the described b step, and its concentrated molecular retention amount is 4000-5000 dalton.
Drying temperature is-10 ℃--5 ℃ in the described b step.
Advantage of the present invention is: abandoned the thermal treatment process of original extraction, temperature is higher, causes the loss of activity of some composition enzymes, in the lycium barbarum polysaccharide, the alkaline polysaccharide that drug action is bad is removed, and keeps effective acidic polysaccharose and neutral polysaccharide, adopt ultrafiltration and toluene wash-out, extraction yield is high.
Embodiment
Following embodiment only is used for explanation the present invention, but can not limit protection scope of the present invention.
Embodiment 1
Wolfberry fruit extract, the weight percent content of polysaccharide is 50% in the extract.
The preparation method of wolfberry fruit extract may further comprise the steps:
A, take the Chinese wolfberry fruit cleaned as raw material, add water, the weight that adds water is 10 times of matrimony vine weight, then the mixture of matrimony vine and water is pulverized, and gets the solid mixture slurries;
B, above-mentioned solid mixture slurries concentrate with ultrafilter, its concentrated molecular retention amount is 4000 dalton, gets concentrated solution, adds the ethanol of volumetric concentration 40% in the concentrated solution, leave standstill, throw out is dissolved in water fully, filters, and filtrate adds the ethanol of volumetric concentration 70%, staticly settle thing, drying, drying temperature is-10 ℃, gets dry thing;
C, get dry thing, the water dissolve complete, carry out ultrafiltration with ultra-filtration equipment, its molecular retention amount is 10000, keeps the solution greater than molecular weight 10000, and solution is concentrated, concentrated solution is adsorbed on the non-polar macroporous resin, with the toluene wash-out of volumetric concentration 60%, reclaim the toluene elutriant, obtain wolfberry fruit extract after dry, concentrated.
Embodiment 2
Wolfberry fruit extract, the weight percent content of polysaccharide is 70% in the extract.
The preparation method of wolfberry fruit extract may further comprise the steps:
A, take the Chinese wolfberry fruit cleaned as raw material, add water, the weight that adds water is 15 times of matrimony vine weight, then the mixture of matrimony vine and water is pulverized, and gets the solid mixture slurries;
B, above-mentioned solid mixture slurries concentrate with ultrafilter, its concentrated molecular retention amount is 4500 dalton, gets concentrated solution, adds the ethanol of volumetric concentration 50% in the concentrated solution, leave standstill, throw out is dissolved in water fully, filters, and filtrate adds the ethanol of volumetric concentration 75%, staticly settle thing, drying, drying temperature is-8 ℃, gets dry thing;
C, get dry thing, the water dissolve complete, carry out ultrafiltration with ultra-filtration equipment, its molecular retention amount is 10000, keeps the solution greater than molecular weight 10000, and solution is concentrated, concentrated solution is adsorbed on the non-polar macroporous resin, with the toluene wash-out of volumetric concentration 65%, reclaim the toluene elutriant, obtain wolfberry fruit extract after dry, concentrated.
Embodiment 3
Wolfberry fruit extract, the weight percent content of polysaccharide is 95% in the extract.
The preparation method of wolfberry fruit extract may further comprise the steps:
A, take the Chinese wolfberry fruit cleaned as raw material, add water, the weight that adds water is 20 times of matrimony vine weight, then the mixture of matrimony vine and water is pulverized, and gets the solid mixture slurries;
B, above-mentioned solid mixture slurries concentrate with ultrafilter, its concentrated molecular retention amount is 5000 dalton, gets concentrated solution, adds the ethanol of volumetric concentration 60% in the concentrated solution, leave standstill, throw out is dissolved in water fully, filters, and filtrate adds the ethanol of volumetric concentration 80%, staticly settle thing, drying, drying temperature is-5 ℃, gets dry thing;
C, get dry thing, the water dissolve complete, carry out ultrafiltration with ultra-filtration equipment, its molecular retention amount is 10000, keeps the solution greater than molecular weight 10000, and solution is concentrated, concentrated solution is adsorbed on the non-polar macroporous resin, with the toluene wash-out of volumetric concentration 70%, reclaim the toluene elutriant, obtain wolfberry fruit extract after dry, concentrated.
Abandoned the thermal treatment process of original extraction, temperature is higher, causes the loss of activity of some composition enzymes, and in the lycium barbarum polysaccharide, the alkaline polysaccharide that drug action is bad is removed, and keeps effective acidic polysaccharose and neutral polysaccharide, adopts ultrafiltration and toluene wash-out, and extraction yield is high.
Claims (5)
1. wolfberry fruit extract, it is characterized in that: the weight percent content of polysaccharide is 50-95% in the described extract.
2. wolfberry fruit extract according to claim 1, it is characterized in that: the weight percent content of polysaccharide is 90-95% in the described extract.
3. the preparation method of wolfberry fruit extract is characterized in that, may further comprise the steps:
A, take the Chinese wolfberry fruit cleaned as raw material, add water, the weight that adds water be matrimony vine weight 10-20 doubly, then the mixture of matrimony vine and water is pulverized, get the solid mixture slurries;
B, above-mentioned solid mixture slurries concentrate with ultrafilter, get concentrated solution, add the ethanol of volumetric concentration 40-60% in the concentrated solution, leave standstill, throw out is dissolved in water fully, filters, and filtrate adds the ethanol of volumetric concentration 70-80%, staticly settle thing, drying gets dry thing;
C, get dry thing, the water dissolve complete, carry out ultrafiltration with ultra-filtration equipment, its molecular retention amount is 10000, keeps the solution greater than molecular weight 10000, and solution is concentrated, concentrated solution is adsorbed on the non-polar macroporous resin, with the toluene wash-out of volumetric concentration 60-70%, reclaim the toluene elutriant, obtain wolfberry fruit extract after dry, concentrated.
4. the preparation method of wolfberry fruit extract according to claim 3, it is characterized in that: ultrafilter concentrates in the described b step, and its concentrated molecular retention amount is 4000-5000 dalton.
5. the preparation method of wolfberry fruit extract according to claim 3, it is characterized in that: drying temperature is-10 ℃--5 ℃ in the described b step.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012104080769A CN102924617A (en) | 2012-10-24 | 2012-10-24 | Chinese wolfberry extract and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012104080769A CN102924617A (en) | 2012-10-24 | 2012-10-24 | Chinese wolfberry extract and preparation method thereof |
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| CN102924617A true CN102924617A (en) | 2013-02-13 |
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| CN2012104080769A Pending CN102924617A (en) | 2012-10-24 | 2012-10-24 | Chinese wolfberry extract and preparation method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103146389A (en) * | 2013-03-21 | 2013-06-12 | 广西壮族自治区中国科学院广西植物研究所 | Method for extracting antioxidant ingredients from eggplant and product |
| CN106243237A (en) * | 2016-08-03 | 2016-12-21 | 安徽徽王农业有限公司 | The manufacture method of blue berry polysaccharide |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101564469A (en) * | 2008-04-23 | 2009-10-28 | 北京卓越同创药物研究院 | Lycium barbarum polysaccharide extract and pharmaceutical application thereof |
| CN102495167A (en) * | 2011-12-09 | 2012-06-13 | 劲牌有限公司 | Method for detecting lycium barbarum polysaccharide in lycium barbarum polysaccharide extract |
| CN102659741A (en) * | 2012-04-27 | 2012-09-12 | 宁夏大学 | Method for extracting and preparing active ingredients of Ningxia wolfberries |
-
2012
- 2012-10-24 CN CN2012104080769A patent/CN102924617A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101564469A (en) * | 2008-04-23 | 2009-10-28 | 北京卓越同创药物研究院 | Lycium barbarum polysaccharide extract and pharmaceutical application thereof |
| CN102495167A (en) * | 2011-12-09 | 2012-06-13 | 劲牌有限公司 | Method for detecting lycium barbarum polysaccharide in lycium barbarum polysaccharide extract |
| CN102659741A (en) * | 2012-04-27 | 2012-09-12 | 宁夏大学 | Method for extracting and preparing active ingredients of Ningxia wolfberries |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103146389A (en) * | 2013-03-21 | 2013-06-12 | 广西壮族自治区中国科学院广西植物研究所 | Method for extracting antioxidant ingredients from eggplant and product |
| CN106243237A (en) * | 2016-08-03 | 2016-12-21 | 安徽徽王农业有限公司 | The manufacture method of blue berry polysaccharide |
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Application publication date: 20130213 |