CN102919890A - Composite, preparation method thereof and food comprising composite - Google Patents
Composite, preparation method thereof and food comprising composite Download PDFInfo
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- CN102919890A CN102919890A CN2012104601395A CN201210460139A CN102919890A CN 102919890 A CN102919890 A CN 102919890A CN 2012104601395 A CN2012104601395 A CN 2012104601395A CN 201210460139 A CN201210460139 A CN 201210460139A CN 102919890 A CN102919890 A CN 102919890A
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- collagen
- chondroitin sulfate
- sea cucumber
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Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Cosmetics (AREA)
Abstract
The invention provides a composite that comprises collagen and chondroitin sulfate and is prepared by taking sea cucumber as a raw material. The composite is a dry powder, wherein collagen accounts for 70-95% of solid composition in mass, and chondroitin sulfate accounts for 5-30% of the solid composition in mass. The invention further provides a preparation method of the composite and food comprising the composite. In the composite, collagen and chondroitin sulfate are high in content. The composite can be added in food to be used. By intaking the composite powder or the food with the composite powder, the effects of beautifying skin, improving function of joints, strengthening bone tissue and resisting against fatigue can be achieved, so that the composite is a product with good market prospect.
Description
Technical field
The present invention relates to a kind of compound that obtains take sea cucumber as raw material and preparation method thereof, and the food that contains this compound.
Background technology
Sea cucumber have nourish strong, strengthen muscle power, prevention of arterial sclerosis, improve the various health cares such as constipation, diuresis, enhancing immunity, valued with regard to being regarded as a kind of healthy food since ancient times.From last century the eighties developed the holothruian cultures technology latter half, centered by Qingdao, Dalian, the holothruian cultures amount annual in the whole nation approximately reached 70,000 tons, becomes a large industry at present.
When sea cucumber was used as food, general eating method was with the rear direct use that gills of fresh sea cucumber; Or sea cucumber is boiled drying makes dried sea-cucumber, be used for cooking after this dried sea-cucumber bubble is sent out or this dried sea-cucumber pulverized after be processed into powder and drink again etc.
Sea cucumber is rich in the healthy functions compositions such as collagen and chondroitin sulfate.Collagen is a kind of white, opaque, unbranched fibroid protein; it extensively is present in the skin, bone, muscle, cartilage, joint, hair tissue of human body; in vivo as " extracellular matrix " composition in tytosis and space between cells; form fibrous or membrane structure; play triple anti-aging effects of support, reparation, protection; can replenish the required nutrition of each layer of skin; make collagen increased activity in the skin, skin care is arranged, delay senility, the effect such as beauty treatment, wrinkle-chasing, hair care.In recent years, use the multiplex do beauty treatment of the collagen food of the productions such as skin, bone and fish skin and scale of domestic animal, in order to improve skin quality.On the other hand, chondroitin sulfate and collagen are with being present in a large number in the animal cartilage as " extracellular matrix " composition, Sulfation also occurs at C-4 position or the C-6 position hydroxyl of N-acetylamino galactosamine in the polysaccharide that the repetition disaccharide unit that is formed by connecting with β-Isosorbide-5-Nitrae-glycosidic bond by D-glucuronic acid and N-acetylamino galactosamine forms.In industrial aspect, chondroitin sulfate is used for pharmaceuticals, cosmetics, the healthy food etc. such as eyedrops and ointment take shark tenterloin cartilage or dog salmon nasal cartilages etc. as the raw material manufacturing.But, up to the present, also not about be rich in the relevant report of the compound of collagen and chondroitin sulfate as raw material prepares take sea cucumber.
Summary of the invention
The purpose of this invention is to provide a kind of take sea cucumber as raw material the compound that is rich in collagen and chondroitin sulfate and the manufacture method thereof applicable to food.In addition, another object of the present invention provides a kind of food that contains above-mentioned compound.
Particularly, compound of the present invention is a kind of compound that contains collagen and chondroitin sulfate that obtains take sea cucumber as raw material, be dried powder, wherein collagen content accounts for 70～95% of solid state component quality, and the chondroitin sulfate cellulose content accounts for 5～30% of solid state component quality.Collagen content preferably accounts for 75～90% of solid state component quality, and the chondroitin sulfate cellulose content preferably accounts for 10～25% of solid state component quality.The number-average molecular weight of collagen is preferably 500～5, and 000, more preferably 2,000～4,000.
Method for the preparation of above-mentioned compound of the present invention comprises following operation:
(1) from sea cucumber, extracts chondroitin sulfate
The water that adds the 100-2000 mass parts in the sea cucumber of 100 mass parts, under 60-100 ℃ temperature, extracted 0.5-3 hour, after extracting end, separation and Extraction liquid and sea cucumber residue filter extract, after filtered fluid being concentrated into the 1/2-1/5 of former liquid measure, stir the lower concentrate liquid measure 2-10 ethanol doubly that adds, produce sediment, left standstill under the normal temperature 3-24 hour, reclaim sediment, obtain containing the sediment of chondroitin sulfate;
(2) enzymolysis sea cucumber residue obtains low molecular collagen
Add the water of 150-500 mass parts and the proteolytic enzyme of 0.5-10 mass parts in the sea cucumber residue that obtains in the operation (1) of 100 mass parts, under 30-70 ℃ temperature enzymolysis 3-24 hour, after enzyme digestion reaction finishes, be heated to more than 80 ℃ and make enzyme deactivation, obtain containing the liquid of low molecular collagen;
(3) obtain containing the compound of collagen and chondroitin sulfate
The sediment that contains chondroitin sulfate that obtains in the operation (1) is dissolved in the liquid that contains low molecular collagen that obtains in the operation (2), after mixed liquor being concentrated into the 1/3-1/10 of former liquid measure, dry concentrate obtains containing the composite powder of collagen and chondroitin sulfate.
In addition, also preferably in operation (1), be included in the concentrate to add before the ethanol with acid the pH of concentrate be adjusted into the step of 1-4, in operation (2), comprise to the enzyme treatment fluid decolour, the step of deodorization processing.Described decolouring, deodorization are processed and are preferably as follows method: with respect to the collagen of 100 mass parts in the enzyme treatment fluid, add the active carbon of 5-30 mass parts, stirred 15 minutes-3 hours under 50-100 ℃ temperature.
Collagen in the compound of the present invention and chondroitin sulfate cellulose content are high, can make an addition in the food and use, by picked-up composite powder of the present invention or added the food of composite powder of the present invention, can obtain skin makeup, improve function of joint, strengthen bone tissue, the health-care effects such as antifatigue are the extraordinary products of a kind of market prospects.
The specific embodiment
Compound of the present invention is a kind of compound that contains collagen and chondroitin sulfate that obtains take sea cucumber as raw material, be dried powder, wherein collagen content accounts for 70～95% of solid state component quality, the chondroitin sulfate cellulose content accounts for 5～30% of solid state component quality, more preferably: collagen content accounts for 75～90% of solid state component quality, and the chondroitin sulfate cellulose content accounts for 10～25% of solid state component quality.
The number-average molecular weight of collagen is preferably 500～5, and 000, more preferably 2,000～4,000.If the number-average molecular weight of collagen is in this molecular weight ranges, because human body has good absorbability to it, and bitter taste is few, and the viscosity when being dissolved in the water is lower, therefore suitable adding in the food.
Compound of the present invention can prepare by the following method.
One, the selection of raw material and processing
In the present invention, be used for the sea cucumber of raw material if belong to the animal of Echinodermata Holothuroidea, then be not particularly limited, it can be wild sea cucumber, it also can be culturing sea cucumbers, for example, stichopus japonicus (Stichopus japonicus Selenka), Holothuria atra (Holothuria atra Jaeger), plum blossom ginseng (Thelenota ananas Jaeger), Stichopus chloronotus (Brandt) (Stichopus chloronotus Brandt), white background footwear ginseng (Actinopyga mauritiana Quoy et Gaimard) etc., but preferably adopt the place of production at DaLian, China and quantitatively can stablize extra large stichopus japonicus and external wild Iceland sea cucumber and the Canadian sea cucumber etc. of acquisition.
Use position to sea cucumber is not particularly limited, and can use whole sea cucumber or internal organ, or each position that gills.Can directly use fresh sea cucumber, also can use the dried sea-cucumber that obtains after the fresh sea cucumber drying.Drying means to sea cucumber is not particularly limited, and can carry out drying by methods such as Exposure to Sunlight, hot-air seasoning, freeze dryings.In addition, sea cucumber can directly be extracted, but in order to improve extraction efficiency, preferably extracts through after cutting off, chopping up, pulverize again.
Two, from sea cucumber, extract chondroitin sulfate
The water that adds the 100-2000 mass parts in the sea cucumber of 100 mass parts, under 60-100 ℃ temperature, extracted 0.5-3 hour, after extracting end, separation and Extraction liquid and sea cucumber residue (can be that the wire netting of 150-200 μ m separates with diameter for example), filter extract, filtered fluid is concentrated into (for example can utilize the reduced pressure concentration machine to concentrate) behind the 1/2-1/5 of former liquid measure, stir the lower doubly ethanol (preferred 95% ethanol) of (preferred 3-5 doubly) of concentrate liquid measure 2-10 that adds, produce sediment, leave standstill 3-24 hour (preferred 5-12 hour) under the normal temperature, reclaim sediment (being that the wire netting of 150-200 μ m is isolated sediment with diameter for example), obtain containing the sediment of chondroitin sulfate.
In addition, can also in concentrate, add before the ethanol with acid (such as hydrochloric acid, lactic acid or citric acid etc.) pH of concentrate is adjusted into 1-4(and is preferably 2～3), will produce easily precipitation like this.
Three, enzymolysis sea cucumber residue obtains low molecular collagen
Then the water that adds the 150-500 mass parts in the sea cucumber residue that obtains in the operation (1) of 100 mass parts add proteolytic enzyme and process the sea cucumber residue, makes the macromolecular collagen protein in the sea cucumber residue become low molecular collagen.Enzyme digestion reaction is heated to more than 80 ℃ and makes enzyme deactivation after finishing, and obtains containing the liquid of low molecular collagen.
Proteolytic enzyme is not particularly limited, can uses neutral proteinase, acid protease, alkali protease or contain complex enzyme formulation of these enzymes etc.Can use normal food to make used commercially available protease, such as papain, neutral proteinase (Novozymes Company's production), hydrolysising protease (Novozymes Company's production) etc.The addition of enzyme, reaction time and treatment temperature can rationally be set, but generally, the addition of enzyme is 0.5～10%, preferred 1～5% for the treatment of fluid solid constituent quality; Reaction time is 3～24 hours, preferred 8～12 hours; Reaction temperature is 30～70 ℃, preferred 45～65 ℃.
Four, to the enzyme treatment fluid decolour, deodorizing processes
When compound of the present invention is used for food, preferably to the enzyme treatment fluid of front decolour, deodorizing processes.The method of decolouring, deodorizing preferably adopts solid absorbent.Solid absorbent can suitably be used active carbon, atlapulgite, synthetic resin, silica gel, alumina etc., preferably uses active carbon.For example, collagen with respect to 100 mass parts in the enzyme treatment fluid, the active carbon that adds 5-30 mass parts (preferred 1-20 mass parts), after stirring 15 minutes-3 hours (preferred 30 minutes-1 hour) under the temperature of 50-100 ℃ (preferred 65-85 ℃), the filtered and recycled filtered fluid gets final product.Also have, also can finish decolouring, deodorizing processing by the pillar of having filled the solid adsorbents such as active carbon being led to liquid.
Five, preparation composite powder
The above-mentioned sediment that contains chondroitin sulfate is joined above-mentioned containing in the liquid that hangs down molecular collagen, stirring makes it dissolving, then the 1/3-1/10(that mixed liquor is concentrated into former liquid measure for example can utilize the reduced pressure concentration machine to concentrate), solid constituent accounts for the 10～50%(preferred 20～40% of quality in the concentrate of this moment).Excessively low such as solid constituent, drying efficiency will variation, and if too high, then viscosity can improve, and makes moisture be difficult to evaporation.Dry available spray drying process, hypobaric drying method, freeze-drying, or make up these methods and carry out.In addition, also can be in advance in stoste, add the excipient such as dextrin and carry out drying.
By the powder that above-mentioned steps obtains, the collagen in its solid constituent accounts for the 70～95%(preferred 75～90% of quality), chondroitin sulfate accounts for the 5～30%(preferred 10～25% of quality).In addition, the number-average molecular weight of collagen is 500～5,000(preferred 2,000～4,000).
Above-mentioned composite powder can add the food that obtains being rich in chondroitin sulfate and low molecular collagen in the food to.The food that adds composite powder of the present invention is not particularly limited, for example, beverage, barbecue dessert, frozen confectionery, candy, jelly, lollipop, jam, chocolate, chewing gum, soup class, bread, various distillage, flesh of fish processed goods, ham, sausage class, flavouring, various health food (tablet, capsule, particle, liquor etc.).Add the amount of composite powder of above-mentioned food to if obtain desired health care, just be not particularly limited, preferably decide suitable addition according to the commodity form, make and to take in compound 50～10 of the present invention common every day, 000mg preferably can take in 1000～5,000mg every day.For example, if solid shape food preferably accounts for 10～50% of quality, if the liquid foods such as distillation food, beverage then preferably account for 1～10% of quality.In addition, composite powder of the present invention also can mix use with the collagen in other sources.For example, in the composite powder of 100 mass parts, add the former albumen of fish scale glue of 10～500 mass parts.
Collagen in the compound of the present invention and chondroitin sulfate cellulose content are high, can make an addition in the food and use, by picked-up composite powder of the present invention or added the food of composite powder of the present invention, can obtain skin makeup, improve function of joint, strengthen bone tissue, the health-care effects such as antifatigue are the extraordinary products of a kind of market prospects.
Embodiment
Describe the present invention in detail below in conjunction with embodiment; but the following examples only are the better embodiment of the present invention; protection scope of the present invention is not limited to this; anyly be familiar with those skilled in the art in the technical scope that the present invention discloses; be equal to replacement or change according to technical scheme of the present invention and inventive concept thereof, all should be encompassed within protection scope of the present invention.
One, preparation compound
Embodiment 1
The bright sea cucumber of 5kg is cut into fritter about 1～3cm, add again 5.5kg water, under 80 ℃ temperature, extracted 1 hour.After with the wire netting of 150 μ m solid-liquid being separated, obtain 8.7kg extract and 1.8kg residue.Behind the Filter paper filtering extract, obtain 8.5kg filtered fluid (Brix2.0).After with the reduced pressure concentration machine that filtered fluid is concentrated, obtain 1.7kg concentrate (Brix9.5).After with watery hydrochloric acid the pH value of concentrate being adjusted to 2.5, Yi Bian stir concentrate, Yi Bian divide (each 1.7L) a small amount of interpolation 3 times with 95% ethanol of 5.1L.After producing precipitation in the liquid, placed at normal temperatures 12 hours.Afterwards, after with the wire netting of 150 μ m sediment being separated, obtain the 68g sediment.
Next, in above-mentioned 1.8kg residue, add 3.54kg water, be heated to again 60 ℃, then add the 10.6g papain, under 55～60 ℃ temperature, carry out 12 hours enzyme digestion reaction.Reaction is warmed up to 80 ℃ after finishing, and makes enzyme killing, and the liquid measure of this moment is 4.7kg(Brix3.6).In this enzyme treatment fluid, add the 34g active carbon (" active carbon 781-A ", be about the collagen quality 20%), under 70 ℃ temperature, stirred 1 hour, carry out deodorizing, decolouring is processed.With filter paper charcoal treatment liquid is filtered, obtains 4.8kg(Brix3.2) filtered fluid.In filtered fluid, add above-mentioned 68g sediment, under 60 ℃ temperature, stirred 1 hour the dissolution precipitation thing.Afterwards, utilize the reduced pressure concentration machine to concentrate, obtain 975g(Brix19.5) concentrate.Utilize freeze drier that this concentrate is carried out drying, obtain the 167g dried powder.
The dried powder that obtains is analyzed, and oven dry decrement (105 ℃, 4 hours) is 4.8%, and the collagen content in the solid constituent (semimicro-Kjeldahl method) is 74.5%, and chondroitin sulfate cellulose content (barium sulfate method) is 19.1%, and ash is 6.4%.In addition, the number-average molecular weight of collagen (high efficiency liquid chromatography) is 2,300.
Embodiment 2
The dry sea cucumber of 1kg is ground into about 1～5mm, adds again 10kg water, under 80 ℃ temperature, extracted 1 hour.After with the wire netting of 150 μ m solid-liquid being separated, obtain 6.1kg extract and 3.2kg residue.Behind the Filter paper filtering extract, obtain 5.9kg filtered fluid (Brix1.6).After with the reduced pressure concentration machine that filtered fluid is concentrated, obtain 1.9kg concentrate (Brix4.6).After with watery hydrochloric acid the pH value of concentrate being adjusted to 2.5, Yi Bian stir concentrate, Yi Bian divide (each 1.9L) a small amount of interpolation 3 times with 95% ethanol of 5.7L.After producing precipitation in the liquid, placed at normal temperatures 12 hours.Afterwards, after with the wire netting of 150 μ m sediment being separated, obtain the 91g sediment.
Next, in above-mentioned 3.2kg residue, add 6.4kg water, be heated to again 60 ℃, then add 12g neutral proteinase (Novozymes Company's production), under 55～60 ℃ temperature, carry out 6 hours enzyme digestion reaction, then with rare caustic soda the pH value is adjusted to 7.0, add 12g alkali protease (Novozymes Company's production), under 55～60 ℃ temperature, carry out again 6 hours enzyme digestion reaction.Reaction is warmed up to 80 ℃ after finishing, and makes enzyme killing, and the liquid measure of this moment is 9.3kg(Brix3.3).In this enzyme treatment fluid, add the 60g active carbon (" active carbon 781-A ", be about the collagen quality 20%), under 70 ℃ temperature, stirred 1 hour, carry out deodorizing, decolouring is processed.With filter paper charcoal treatment liquid is filtered, obtains 8.9kg(Brix3.2) filtered fluid.In filtered fluid, add above-mentioned 91g sediment, under 60 ℃ temperature, stirred 1 hour the dissolution precipitation thing.Afterwards, utilize the reduced pressure concentration machine to concentrate, obtain 1.7kg(Brix20.8) concentrate.Utilize spray dryer that this concentrate is carried out drying, obtain the 293g dried powder.
The dried powder that obtains is analyzed, and oven dry decrement (105 ℃, 4 hours) is 3.8%, and the collagen content in the solid constituent (semimicro-Kjeldahl method) is 72.0%, and chondroitin sulfate cellulose content (barium sulfate method) is 22.7%, and ash is 5.3%.In addition, the number-average molecular weight of collagen (high efficiency liquid chromatography) is 1,800.
Embodiment 3
The dry sea cucumber of 100kg is ground into about 1～5mm, adds again 1,000kg water, under 80 ℃ temperature, extracted 1 hour.After with the wire netting of 150 μ m solid-liquid being separated, obtain 620kg extract and 356kg residue.Behind the Filter Press extract, obtain 610kg filtered fluid (Brix1.8).After with the reduced pressure concentration machine that filtered fluid is concentrated, obtain 210kg concentrate (Brix5.2).After with watery hydrochloric acid the pH value of concentrate being adjusted to 2.7, Yi Bian stir concentrate, Yi Bian divide (each 200L) a small amount of interpolation 3 times with 95% ethanol of 600L.After producing precipitation in the liquid, placed at normal temperatures 12 hours.Afterwards, after with the wire netting of 150 μ m sediment being separated, obtain the 8.7kg sediment.
Next, in above-mentioned 356kg residue, add 640kg water, be heated to again 60 ℃, then add 1.5kg neutral proteinase (Novozymes Company's production), under 55～60 ℃ temperature, carry out 6 hours enzyme digestion reaction, then with rare caustic soda the pH value is adjusted to 7.0, add 1.5kg alkali protease (Novozymes Company's production), under 55～60 ℃ temperature, carry out again 6 hours enzyme digestion reaction.Reaction is warmed up to 80 ℃ after finishing, and makes enzyme killing, and the liquid measure of this moment is 940kg(Brix4.7).In this enzyme treatment fluid, add the 8.8kg active carbon (" active carbon 781-A ", be about the collagen quality 20%), under 70 ℃ temperature, stirred 1 hour, carry out deodorizing, decolouring is processed.With filter paper charcoal treatment liquid is filtered, obtains 905kg(Brix4.5) filtered fluid.In filtered fluid, add above-mentioned 8.7kg sediment, under 60 ℃ temperature, stirred 1 hour the dissolution precipitation thing.Afterwards, utilize the reduced pressure concentration machine to concentrate, obtain 192kg(Brix21.2) concentrate.Utilize spray dryer that this concentrate is carried out drying, obtain the 42.3kg dried powder.
The dried powder that obtains is analyzed, and oven dry decrement (105 ℃, 4 hours) is 4.9%, and the collagen content in the solid constituent (semimicro-Kjeldahl method) is 72.8%, and chondroitin sulfate cellulose content (barium sulfate method) is 19.3%, and ash is 7.9%.In addition, the number-average molecular weight of collagen (high efficiency liquid chromatography) is 1,950.
Two, preparation contains the food of compound
1. tablet
Take by weighing the former protein 20 .0% of compound 50.0%, fish scale glue, lactose 23.5%, apple aroma spices 0.5%, vitamin premix (B1:B2:B6=4:3:3) 3.0%, the sucrose oleate 3.0% that each raw material: embodiment 1 obtains by following quality proportioning, after evenly mixing, make the round tablet of 250mg/ sheet of tablet press machine.
2. beverage
Take by weighing the former albumen 5.0% of compound 2.0%, fish scale glue, vitamin C 0.6%, vitamin B2 0.01%, fructose Glucose Liquid sugar 10.5%, citric acid 1.2%, Aspartame 0.1%, strawberry flavor spices 0.1%, the water 80.5% that each raw material: embodiment 2 obtains by following quality proportioning, make according to a conventional method beverage.
3. barbecue dessert
Take by weighing the former albumen 2.5% of compound 2.5%, fish scale glue, weak strength flour 40.0%, yeast powder 0.3%, cream 20.0%, egg 10.0%, the granulated sugar 24.7% that each raw material: embodiment 3 obtains by following quality proportioning, after making dough, fermentation, barbecue was made into barbecue dessert in 15 minutes under 180 ℃ temperature.
4. particle
Take by weighing the former albumen 79.5% of compound 20.0%, fish scale glue, the melon glue 0.5% that each raw material: embodiment 3 obtains by following quality proportioning, after evenly mixing, make particle with fluidized granulation mechanism.
Claims (9)
1. compound, it is characterized in that, be a kind of compound that contains collagen and chondroitin sulfate that obtains take sea cucumber as raw material, is dried powder, wherein collagen content accounts for 70～95% of solid state component quality, and the chondroitin sulfate cellulose content accounts for 5～30% of solid state component quality.
2. compound according to claim 1 is characterized in that, collagen content accounts for 75～90% of solid state component quality, and the chondroitin sulfate cellulose content accounts for 10～25% of solid state component quality.
3. compound according to claim 1 is characterized in that, the number-average molecular weight of collagen is 500～5,000.
4. compound according to claim 3 is characterized in that, the number-average molecular weight of collagen is 2,000～4,000.
5. a method for preparing the described compound of any one among the claim 1-4 is characterized in that, comprises following operation:
(1) from sea cucumber, extracts chondroitin sulfate
The water that adds the 100-2000 mass parts in the sea cucumber of 100 mass parts, under 60-100 ℃ temperature, extracted 0.5-3 hour, after extracting end, separation and Extraction liquid and sea cucumber residue filter extract, after filtered fluid being concentrated into the 1/2-1/5 of former liquid measure, stir the lower concentrate liquid measure 2-10 ethanol doubly that adds, produce sediment, left standstill under the normal temperature 3-24 hour, reclaim sediment, obtain containing the sediment of chondroitin sulfate;
(2) enzymolysis sea cucumber residue obtains low molecular collagen
Add the water of 150-500 mass parts and the proteolytic enzyme of 0.5-10 mass parts in the sea cucumber residue that obtains in the operation (1) of 100 mass parts, under 30-70 ℃ temperature enzymolysis 3-24 hour, after enzyme digestion reaction finishes, be heated to more than 80 ℃ and make enzyme deactivation, obtain containing the liquid of low molecular collagen;
(3) obtain containing the compound of collagen and chondroitin sulfate
The sediment that contains chondroitin sulfate that obtains in the operation (1) is dissolved in the liquid that contains low molecular collagen that obtains in the operation (2), after mixed liquor being concentrated into the 1/3-1/10 of former liquid measure, dry concentrate obtains containing the composite powder of collagen and chondroitin sulfate.
6. method according to claim 5 is characterized in that, also is included in the operation (1) to add the front step that the pH of concentrate is adjusted into 1-4 with acid of ethanol in the concentrate.
7. method according to claim 5 is characterized in that, also comprises the step to the enzyme treatment fluid decolours, deodorization is processed in the operation (2).
8. method according to claim 7 is characterized in that, with respect to the collagen of 100 mass parts in the enzyme treatment fluid, adds the active carbon of 5-30 mass parts, stirs 15 minutes-3 hours under 50-100 ℃ temperature, decolours, deodorization processes.
9. a food is characterized in that, contains the described compound of any one among the claim 1-4.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104172189A (en) * | 2014-08-26 | 2014-12-03 | 淮安麦德森制药有限公司 | Jelly containing chondroitin sulfate |
| WO2015070386A1 (en) * | 2013-11-13 | 2015-05-21 | 青岛贝尔特生物科技有限公司 | Dietary nutritional supplement for preventing elderly osteoarticular diseases |
| CN106929555A (en) * | 2017-03-29 | 2017-07-07 | 山东圣洲海洋生物科技股份有限公司 | A kind of preparation method of sea cucumber enzymolysis alcohol extracting component and its alcohol extracting component for preparing and application |
| CN109105702A (en) * | 2018-08-30 | 2019-01-01 | 泓博元生命科技（深圳）有限公司 | A kind of preparation method of composition containing NADH, application and energy extender |
| CN109170907A (en) * | 2018-08-30 | 2019-01-11 | 泓博元生命科技（深圳）有限公司 | A kind of preparation method of composition containing NMN, application and sports drink |
| CN112352891A (en) * | 2020-10-27 | 2021-02-12 | 安徽济生元药业有限公司 | Sea sipunculus and sea cucumber polypeptide drink and preparation method thereof |
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| WO2015070386A1 (en) * | 2013-11-13 | 2015-05-21 | 青岛贝尔特生物科技有限公司 | Dietary nutritional supplement for preventing elderly osteoarticular diseases |
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| CN106929555B (en) * | 2017-03-29 | 2020-05-15 | 山东圣洲海洋生物科技股份有限公司 | Preparation method of sea cucumber enzymolysis-alcohol extraction component, alcohol extraction component prepared by preparation method and application of alcohol extraction component |
| CN109105702A (en) * | 2018-08-30 | 2019-01-01 | 泓博元生命科技（深圳）有限公司 | A kind of preparation method of composition containing NADH, application and energy extender |
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| CN112352891A (en) * | 2020-10-27 | 2021-02-12 | 安徽济生元药业有限公司 | Sea sipunculus and sea cucumber polypeptide drink and preparation method thereof |
| CN112352891B (en) * | 2020-10-27 | 2023-09-12 | 安徽济生元药业有限公司 | Sipunculus nudus and sea cucumber polypeptide drink and preparation method thereof |
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