CN102919128A - Out-of-bottle bag rooting method for tissue culture seedlings of Europe and America hybrid aspens - Google Patents
Out-of-bottle bag rooting method for tissue culture seedlings of Europe and America hybrid aspens Download PDFInfo
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- CN102919128A CN102919128A CN2012104569559A CN201210456955A CN102919128A CN 102919128 A CN102919128 A CN 102919128A CN 2012104569559 A CN2012104569559 A CN 2012104569559A CN 201210456955 A CN201210456955 A CN 201210456955A CN 102919128 A CN102919128 A CN 102919128A
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Abstract
The invention relates to an out-of-bottle bag rooting method for tissue culture seedlings of Europe and America hybrid aspens. The method is to the problem that the cultivation cost is high, the production phase is long and the survival rate of transplanting of tissue culture seedlings is low in a tissue culture process of the Europe and America hybrid aspens. The method comprises the following steps: 1, plastically packaging the bottom of polypropylene plastic bags with a sealing machine to manufacture rooting culture bags; 2, mixing peat soils with sands, sterilizing at high speed to obtain composts and packing the composts into the rooting culture bags; 3, burrowing in the composts in the culture bags and wetting each hole with rooting culture solution; and 4, taking out the tissue culture seedlings of the Europe and America hybrid aspens from culture mediums, planting the tissue culture seedlings into the holes of the culture bags, sealing the top of the culture bags with the sealing machine, placing in greenhouses to culture for 5-7 days, gashing the top of the culture bags and then culturing for 8-10 days to obtain the rooting seedlings of the Europe and America hybrid aspens. The method is used in the field of asexual propagation of the Europe and America hybrid aspens.
Description
Technical field
The present invention relates to a kind of American-European hybrid aspen group training seedling rooting method.
Background technology
(Populus tremula * P.tremuloides) is the crossbreed of trembling poplar (P.tremula) and North America aspen (P.tremuloides) to American-European hybrid aspen, have that growth is fast, cold-resistant, the characteristics such as impoverishment tolerant, tree-like tall and big, strong adaptability, be the fine tree species of warm frigid zone planting out.In addition, this hybrid material is softer, fine and closely woven, pure white, and the trunk end is straight, is the quality raw materials of the industry such as papermaking, plywood, particle board.
Studies show that: in Finland, American-European aspen hybrid is suitable for planting out, and Plain afforestation growing way is better, and its rotation and growth rate are all faster than general mountain region pin broad-leaved reproducting tree species.And the climatic environment in a lot of areas of northern China is better than Finland, fertile soil, and temperature is high, and sunshine is long, and vegetative period is also long, directly adopts and introduces good aspen hybrid clonal planting, and its amount of growth is more remarkable.Therefore, these seeds be suitable in south, China Heilungkiang, Jilin, Liaoning and North China's plantation.Extensively introduced by many countries as good commerical tree species at present.China is in 2000 and calendar year 2001, introduce 50 of American-European aspen hybrid choiceness by Forest Tree Genetics and Breeding section of Northeast Forestry University from Finland, method by group training and cuttage is carried out amount reproduction and the afforestation of going up a hill, purpose is the forest resources of abundant China, for forestry and forest product industry are brought larger ecology and economic benefit.
But in the vegetative technology implementation process of American-European hybrid aspen, be faced with that culture medium cost is high, the production cycle is long, the problems such as group training transplantation of seedlings survival rate is low, the problem that wherein is difficult for surviving with transplanting is the most serious, cause planting percent low, seedling cost increases, and has had a strong impact on the large-area popularizing planting of American-European hybrid aspen.Therefore, how to improve American-European hybrid aspen group training transplantation of seedlings survival rate and shortening growing-seedling period and become the task of top priority.
Summary of the invention
The present invention will solve to exist in the existing American-European hybrid aspen tissue culture procedures to cultivate that cost is high, the production cycle is long, and the low problem of group training transplantation of seedlings survival rate provides a kind of American-European hybrid aspen group to train the method that seedling bottle outer bag is taken root.
The method that the American-European hybrid aspen group of the present invention training seedling bottle outer bag is taken root may further comprise the steps:
One, with wide be 17～20cm, height is the bottom sealing machine plastic packaging of the polypropylene plastics pocket of 10～12cm, is made into the culture of rootage bag;
Two, peat soil and sand are mixed by volume at 1～2: 1, then carried out high-temperature sterilization 1.5～2 hours in 121 ℃, get composts or fertilisers of cultivating, then composts or fertilisers of cultivating is sub-packed in the culture of rootage bag, the charge weight of composts or fertilisers of cultivating is 1/3 of culture bag volume in each culture of rootage bag;
Three, the composts or fertilisers of cultivating in the culture bag is dug the cave, the cave specification is wide 1.5～2cm, dark 1.5cm, and then interval 2cm between cave and the cave uses culture of rootage liquid that each cave is wetting, and the consumption of culture of rootage liquid is 3～5mL in each cave;
Four, the American-European hybrid aspen group training seedling with 1.5～3cm takes out from medium, callus excision with group training shoot root section, getting single the group training seedling of excision behind the callus plants in the cave of culture bag, then the culture bag top is sealed with sealing machine, place in the green house and cultivate, illumination is scattered light, temperature of shed is controlled between 23～28 ℃, relative air humidity remains on 60%～70%, cultivate after 5～7 days, the culture bag top is scratched, cultivated again 8～10 days after uncovered, namely get the American-European hybrid aspen seedling of taking root; Culture of rootage liquid described in the step 3 is to contain the WPM medium of 0.3mg/L NAA or contain 0.3mg/L NAA and the WPM medium of 0.05mg/L IAA.
The advantage of the inventive method is as follows:
1. production cost is low: the inventive method is directly planted unrooted group training seedling in peat soil and the husky mixed-matrix and is allowed it take root, utilize natural lighting, need not labor management, not only having reduced in vitro rooting needs man-hour and the energy consumption of sterile working, also reduced the material energy and the man-hour of subculture making and expended, can greatly reduce production costs;
Traditional mode of taking root is to carry out (i.e. bottle in take root) in vitro, and its production cost is high, is mainly manifested in equipment investment large, the aspects such as the large and culture medium cost height of power consumption.And in the high volume production process, often cause polluting because of operate miss.Tissue culture room both domestic and external all is totally enclosed, satisfy test-tube plantlet to the requirement of illumination and temperature by artificial lighting and air-conditioning, the power consumption of this culturing room is large, and the electricity charge account for 50% of whole cost, and the agar in the medium not only consumption is large but also price is very high.The production cost of the inventive method has remarkable reduction than conventional art, can realize suitability for industrialized production.
2. growing-seedling period is short: adopt of the present invention group of training seedling bottle outer bag rooting method, need not through the hardening process, rooting of vitro seedling and domestication are organically combined, simplified the cultivation program, effectively shortened growing-seedling period, the growth of American-European hybrid aspen unrooted group training seedling can be taken root in 15-20 days, transplanted.
Conventional group training seedling rooting all is to carry out in bottle, needs before the transplanting through the hardening process, and its cycle is long, and program is complicated.The inventive method does not need hardening in the transplanting process, seedling raise period has shortened half.
3. transplanting survival rate is high: the present invention adopts bottle outer bag rooting method to replace the technology of taking root in traditional group training seedling bottle, the pollution that the agar of having avoided root system to adhere to causes is rotted, and bottle outer bag rooting method has progressively adapted to external environment, high-output stress-resistance in rooting process.In addition, adopt this method seedling of taking root to transplant together with matrix, do not need slow seedling process, do not have the root system damage, be equivalent to container nursery, transplanting survival rate can reach 80%-95%.
Transplanting survival rate is whether successful key of check group training, also is that can the check group train the key that carry out batch production.Test tube is two separate processes without taking root with outer transplanting of bottle in the offspring bottle, and its rooting rate and transplanting survival rate are to need to add up to calculate continuously.And the outside sprout-cultivating-bottle rate is to take root and transplanting unites two into one.Therefore, the rear transplanting survival rate of taking root in the transplanting survival rate ratio bottle that the inventive method test-tube plantlet bottle outer bag is taken root has significantly raising.
In a word, the American-European hybrid aspen group of the present invention training seedling bottle outer bag rooting method has simple to operate, and growing-seedling period is short, and production cost is low, and transplanting survival rate is high, but the advantages such as batch production production.
Description of drawings
Fig. 1 is the photo that will organize the callus excision of training shoot root section in embodiment seven step 4; Fig. 2 gets single the group training seedling of excision behind the callus to plant photo in the cave of culture bag in embodiment seven step 4; Fig. 3 is the photo of in embodiment seven step 4 culture bag top being sealed with sealing machine; Fig. 4 is American-European hybrid aspen that embodiment seven the obtains seedling photo of taking root.
Embodiment
Technical solution of the present invention is not limited to following cited embodiment, also comprises any combination between each embodiment.
Embodiment one: the method that the American-European hybrid aspen group of present embodiment training seedling bottle outer bag is taken root may further comprise the steps:
One, with wide be 17～20cm, height is the bottom sealing machine plastic packaging of the polypropylene plastics pocket of 10～12cm, is made into the culture of rootage bag;
Two, peat soil and sand are mixed by volume at 1～2: 1, then carried out high-temperature sterilization 1.5～2 hours in 121 ℃, get composts or fertilisers of cultivating, then composts or fertilisers of cultivating is sub-packed in the culture of rootage bag, the charge weight of composts or fertilisers of cultivating is 1/3 of culture bag volume in each culture of rootage bag;
Three, the composts or fertilisers of cultivating in the culture bag is dug the cave, the cave specification is wide 1.5～2cm, dark 1.5cm, and then interval 2cm between cave and the cave uses culture of rootage liquid that each cave is wetting, and the consumption of culture of rootage liquid is 3～5mL in each cave;
Four, the American-European hybrid aspen group training seedling with 1.5～3cm takes out from medium, callus excision with group training shoot root section, getting single the group training seedling of excision behind the callus plants in the cave of culture bag, then the culture bag top is sealed with sealing machine, place in the green house and cultivate, illumination is scattered light, temperature of shed is controlled between 23～28 ℃, relative air humidity remains on 60%～70%, cultivate after 5～7 days, the culture bag top is scratched, cultivated again 8～10 days after uncovered, namely get the American-European hybrid aspen seedling of taking root; Culture of rootage liquid described in the step 3 is to contain the WPM medium of 0.3mg/L NAA or contain 0.3mg/L NAA and the WPM medium of 0.05mg/L IAA.
Present embodiment is drawn from the Finnish Forestry academy of sciences by described American-European hybrid aspen.
Described American-European hybrid aspen group training seedling by the method for Plant Tissue Breeding, divides the new American-European hybrid aspen plant that bears individual for utilizing explant.
Embodiment two: what present embodiment and embodiment one were different is: be 18cm with wide in the step 1, height is the bottom sealing machine plastic packaging of the polypropylene plastics pocket of 11cm.Other is identical with embodiment one.
Embodiment three: what present embodiment was different from embodiment one or two is: in the step 2 peat soil and sand were mixed in 1: 1 by volume.Other is identical with embodiment one or two.
Embodiment four: what present embodiment was different from embodiment one or two is: in the step 2 peat soil and sand were mixed in 2: 1 by volume.Other is identical with embodiment one or two.
Embodiment five: what present embodiment was different from one of embodiment one to four is: in the step 3 in each cave the consumption of culture of rootage liquid be 4mL.Other is identical with one of embodiment one or four.
Embodiment six: what present embodiment was different from one of embodiment one to five is: temperature of shed is controlled at 25 ℃ in the step 4, and relative air humidity remains on 65%.Other is identical with one of embodiment one to five.
Embodiment seven: the method that the American-European hybrid aspen group of present embodiment training seedling bottle outer bag is taken root may further comprise the steps:
One, with wide be 17～20cm, height is the bottom sealing machine plastic packaging of the polypropylene plastics pocket of 10～12cm, is made into the culture of rootage bag;
Two, peat soil and sand were mixed in 1: 1 by volume, then carried out high-temperature sterilization 2 hours in 121 ℃, get composts or fertilisers of cultivating, then composts or fertilisers of cultivating is sub-packed in the culture of rootage bag, the charge weight of composts or fertilisers of cultivating is 1/3 of culture bag volume in each culture of rootage bag;
Three, the composts or fertilisers of cultivating in the culture bag is dug the cave, the cave specification is wide 1.5cm, dark 1.5cm, and then interval 2cm between cave and the cave uses culture of rootage liquid that each cave is wetting, and the consumption of culture of rootage liquid is 4mL in each cave;
Four, the American-European hybrid aspen group training seedling with 2cm takes out from medium, callus excision (as shown in Figure 1) with group training shoot root section, getting single the group training seedling of excision behind the callus plants in the cave of culture bag (as shown in Figure 2), then (as shown in Figure 3) sealed with sealing machine in the culture bag top, place in the green house and cultivate, illumination is scattered light, temperature of shed is controlled at 25 ℃, relative air humidity remains on 65%, cultivate after 6 days, the culture bag top is scratched, cultivated again 9 days after uncovered, namely get the American-European hybrid aspen seedling of taking root.
Culture of rootage liquid described in the step 3 is to contain the WPM medium of 0.3mg/L NAA or contain 0.3mg/LNAA and the WPM medium of 0.05mg/L IAA.
The concrete constituent of described WPM medium is as follows:
The American-European hybrid aspen that present embodiment obtains takes root seedling as shown in Figure 4.The American-European hybrid aspen that present embodiment the is obtained seedling of taking root is transplanted, and moves into through in the matrix of disinfecting together with composts or fertilisers of cultivating during transplanting.Will control environmental condition in the booth after the transplanting well, temperature is controlled at 18～26 ℃, and relative air humidity is more than 60%, and illumination is about 3000Lx.Carry out foliar spray 2-3 every day, and transplanting survival rate can reach 91.5%.
As experimental group, compare simultaneously test with present embodiment, control group adopts conventional organization to cultivate rooting method (namely taking root in the bottle).Comparative test result sees Table 1.
Table 1 bottle outer bag take root and bottle in the effect of taking root relatively
From comparative test result as seen, experimental group is all obviously to be better than control group from aspects such as transplanting survival rate of nursery stocks, plant growing way or growth cycles.
Claims (6)
1. American-European hybrid aspen group training seedling bottle outer bag rooting method is characterized in that the method that American-European hybrid aspen group training seedling bottle outer bag is taken root, and may further comprise the steps:
One, with wide be 17～20cm, height is the bottom sealing machine plastic packaging of the polypropylene plastics pocket of 10～12cm, is made into the culture of rootage bag;
Two, peat soil and sand are mixed by volume at 1～2: 1, then carried out high-temperature sterilization 1.5～2 hours in 121 ℃, get composts or fertilisers of cultivating, then composts or fertilisers of cultivating is sub-packed in the culture of rootage bag, the charge weight of composts or fertilisers of cultivating is 1/3 of culture bag volume in each culture of rootage bag;
Three, the composts or fertilisers of cultivating in the culture bag is dug the cave, the cave specification is wide 1.5～2cm, dark 1.5cm, and then interval 2cm between cave and the cave uses culture of rootage liquid that each cave is wetting, and the consumption of culture of rootage liquid is 3～5mL in each cave;
Four, the American-European hybrid aspen group training seedling with 1.5～3cm takes out from medium, callus excision with group training shoot root section, getting single the group training seedling of excision behind the callus plants in the cave of culture bag, then the culture bag top is sealed with sealing machine, place in the green house and cultivate, illumination is scattered light, temperature of shed is controlled between 23～28 ℃, relative air humidity remains on 60%～70%, cultivate after 5～7 days, the culture bag top is scratched, cultivated again 8～10 days after uncovered, namely get the American-European hybrid aspen seedling of taking root; Culture of rootage liquid described in the step 3 is to contain the WPM medium of 0.3mg/L NAA or contain 0.3mg/L NAA and the WPM medium of 0.05mg/L IAA.
2. American-European hybrid aspen group training seedling bottle outer bag rooting method according to claim 1, it is characterized in that in the step 1 will the wide 18cm of being, and height is the bottom sealing machine plastic packaging of the polypropylene plastics pocket of 11cm.
3. American-European hybrid aspen group training seedling bottle outer bag rooting method according to claim 1 and 2 is characterized in that in the step 2 peat soil and sand being mixed in 1: 1 by volume.
4. American-European hybrid aspen group training seedling bottle outer bag rooting method according to claim 1 and 2 is characterized in that in the step 2 peat soil and sand being mixed in 2: 1 by volume.
5. American-European hybrid aspen group training seedling bottle outer bag rooting method according to claim 3 is characterized in that in the step 3 that the consumption of culture of rootage liquid is 4mL in each cave.
6. American-European hybrid aspen group training seedling bottle outer bag rooting method according to claim 5 is characterized in that temperature of shed is controlled at 25 ℃ in the step 4, and relative air humidity remains on 65%.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103636498A (en) * | 2013-11-29 | 2014-03-19 | 黑龙江省林业科学研究所 | Populus alba * pobulus davidiana dode 1333 tissue culture medium of pobulus davidiana dode improved variety |
| CN107343443A (en) * | 2017-08-14 | 2017-11-14 | 高健强 | A kind of Talinum crassifolium large-scale method for producing |
| CN108651276A (en) * | 2017-03-29 | 2018-10-16 | 北京林业大学 | Rapid Rooting cultural method in blueberry tissue bag |
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| CN103636498A (en) * | 2013-11-29 | 2014-03-19 | 黑龙江省林业科学研究所 | Populus alba * pobulus davidiana dode 1333 tissue culture medium of pobulus davidiana dode improved variety |
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| CN107343443A (en) * | 2017-08-14 | 2017-11-14 | 高健强 | A kind of Talinum crassifolium large-scale method for producing |
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Application publication date: 20130213 |