CN102902055A - Glass slide specimen producing method for microscope photographing of phytoplankton - Google Patents
Glass slide specimen producing method for microscope photographing of phytoplankton Download PDFInfo
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- CN102902055A CN102902055A CN2012103094656A CN201210309465A CN102902055A CN 102902055 A CN102902055 A CN 102902055A CN 2012103094656 A CN2012103094656 A CN 2012103094656A CN 201210309465 A CN201210309465 A CN 201210309465A CN 102902055 A CN102902055 A CN 102902055A
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- China
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- phytoplankton
- microscope
- sample
- microslide
- pictures
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Abstract
The invention relates to a glass slide specimen producing method for microscope photographing of phytoplankton and relates to the field of microscope specimen producing. According to the glass slide specimen producing method, 0.1ml/L of the phytoplankton which is taken for microscope photographing of glass slide specimen manufacturing in traditional methods is improved into 0.4ml/L, and the defect that photographs obtained when 0.1ml/L of the phytoplankton is taken for microscope photographing in the traditional methods are low in definition is overcome by means of the fact that refraction and blocking effects of water on light are increased with the increasing of thickness of water. The method is capable of simply and effectively improving clarity of the photographs obtained by a microscope.
Description
Technical field
The invention provides the microslide Slide processing that a kind of phytoplankton microscope is taken pictures, relate to microscope specimen and make the field.
Background technology
China's economy has obtained unprecedented growing continuously and fast since the reform and opening-up, still, because the rapid expansion of development of resources and the rapid growth of energy resource consumption cause the very serious ecological disruption of China and environmental pollution.In the last few years, along with the eutrophication of China's water body, the breeding of phytoplankton raised growth formed wawter bloom or red tide, caused to the environment and economy development of China to seriously influence and lose.In this simultaneously, other country of the world also faces same problem, so phytoplankton becomes the study hotspot in China and the world.When carrying out phytoplankton research, need to carry out record to the form of phytoplankton, so need to take pictures to phytoplankton, also provide foundation and proof for result of study simultaneously.But the sharpness of taking pictures that the method for at present routine absorption 0.1mL sample making microscope specimen obtains is not high, thereby can not accurately He effectively carry out record to the phytoplankton form, provides clearly and takes pictures.Yet be not that the sample drawn is more few better when making the phytoplankton microscope specimen, when draw sample very little the time cover glass too little with the space of microslide, can push sample, make the sample distortion, thereby impact research or the accuracy of testing.
Summary of the invention
The purpose of this invention is to provide a kind of take pictures microslide Slide processing of photo sharpness of phytoplankton microscope that improves.
Technical scheme of the present invention: the microslide Slide processing that a kind of phytoplankton microscope is taken pictures, step is as follows:
A, choose the phytoplankton sample;
Before b, the sampling sample is shaken up, draw 0.04 ml/L phytoplankton sample, then sample is placed on the center of microslide and with cover glass building gently, finishes the making of phytoplankton sample;
C, the phytoplankton sample that step b is made are placed on the microscopical sample stage;
D, unlatching microscope are adjusted microscope brightness, and microscope is adjusted to focus state;
E, open computer, open the corresponding microscope software of taking pictures;
F, choose the image that to take pictures, image is adjusted accordingly, selected digital image is taken pictures;
G, to taking pictures photo name and select needed picture format, be kept in the file of own appointment.
Employed microslide is of a size of 76.2 * 25.4 * 1mm, and the lid fragmentation is of a size of 22 * 22 * 0.17mm.
Described method is except applying to phytoplankton, can also apply to the observation of other similar object and takes pictures.
Beneficial effect of the present invention: the present invention studies show that phytoplankton making microscope specimen absorption sample 0.04mL is advisable, and the accuracy that neither affects sample record can obtain again the photo of high definition.The inventive method does not need to add any device, only needs to change the amount of drawing sample on original basis, just can effectively improve the take pictures sharpness of gained photo of microscope, simple to operate, easy grasp that the inventive method has, the advantage such as simply effective.
Embodiment
Take the phytoplankton sample that gathers from Taihu Lake as example, as follows operation:
A, choose and need to be ready to observe, phytoplankton sample and the related experiment material of Taking Pictures recording;
Before b, the sampling sample is shaken up, draw 0.04 ml/L phytoplankton sample with liquid-transfering gun, then sample is placed on the center of microslide and with cover glass building gently, finishes the making of phytoplankton sample;
C, OLYMPUS CX31 micro objective is transferred to low power lens first, then the phytoplankton sample is placed on the microscopical objective table;
D, unlatching OLYMPUS CX31 microscope are adjusted microscope brightness, regulate microscope coarse adjustment and fine setting, and microscope is adjusted to focus state;
E, open computer, open the microscope software QCapture Pro that takes pictures;
F, the Preview that opens QImaging Digital Camera carry out preview to MIcrosope image, and choosing needs the image of taking pictures, and are adjusted to optimal brightness and sharpness, click Snap selected digital image is taken pictures;
G, at last will take pictures photo name and select needed picture format are kept in the own specified folder.
Claims (3)
1. microslide Slide processing that the phytoplankton microscope is taken pictures, it is characterized in that: step is as follows:
A, choose the phytoplankton sample;
Before b, the sampling sample is shaken up, draw 0.04 mL/L phytoplankton sample, then sample is placed on the center of microslide and with cover glass building gently, finishes the making of phytoplankton sample;
C, the phytoplankton sample that step b is made are placed on the microscopical objective table;
D, unlatching microscope are adjusted microscope brightness, and microscope is adjusted to focus state;
E, open computer, open the corresponding microscope software of taking pictures;
F, choose the image that to take pictures, image is adjusted accordingly, selected digital image is taken pictures;
G, to taking pictures photo name and select needed picture format, be kept in the file of own appointment.
2. the phytoplankton microscope according to claim 1 microslide Slide processing of taking pictures, it is characterized in that: employed microslide is of a size of 76.2 * 25.4 * 1mm, and the lid fragmentation is of a size of 22 * 22 * 0.17mm.
3. the phytoplankton microscope according to claim 1 microslide Slide processing of taking pictures is characterized in that: described method is except applying to phytoplankton, can also apply to the observation of other similar object and takes pictures.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012103094656A CN102902055A (en) | 2012-08-28 | 2012-08-28 | Glass slide specimen producing method for microscope photographing of phytoplankton |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012103094656A CN102902055A (en) | 2012-08-28 | 2012-08-28 | Glass slide specimen producing method for microscope photographing of phytoplankton |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102902055A true CN102902055A (en) | 2013-01-30 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012103094656A Pending CN102902055A (en) | 2012-08-28 | 2012-08-28 | Glass slide specimen producing method for microscope photographing of phytoplankton |
Country Status (1)
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| CN (1) | CN102902055A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105532651A (en) * | 2015-12-15 | 2016-05-04 | 上海师范大学 | Method for making permanent glass slide specimen of bryophyte |
Citations (6)
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| CN1277394A (en) * | 2000-07-05 | 2000-12-20 | 徐秀林 | Recognition system and analysis method for microscopic biological and medical images |
| CN201149737Y (en) * | 2007-05-18 | 2008-11-12 | 鲁东大学 | Seashell larva integral specimen prepared by concave slide |
| JP2009036970A (en) * | 2007-08-01 | 2009-02-19 | Nikon Corp | Cover glass, slide glass, preparation, observing method and microscope device |
| JP2009036969A (en) * | 2007-08-01 | 2009-02-19 | Nikon Corp | Cover glass, slide glass, preparation, observation method, and microscopic device |
| CN101493398A (en) * | 2009-03-04 | 2009-07-29 | 大庆油田有限责任公司 | Emulsified crude oil emulsion laser co-focussing analytical method |
| CN102499235A (en) * | 2011-11-08 | 2012-06-20 | 哈尔滨师范大学 | Preparation method of diatom sample |
-
2012
- 2012-08-28 CN CN2012103094656A patent/CN102902055A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1277394A (en) * | 2000-07-05 | 2000-12-20 | 徐秀林 | Recognition system and analysis method for microscopic biological and medical images |
| CN201149737Y (en) * | 2007-05-18 | 2008-11-12 | 鲁东大学 | Seashell larva integral specimen prepared by concave slide |
| JP2009036970A (en) * | 2007-08-01 | 2009-02-19 | Nikon Corp | Cover glass, slide glass, preparation, observing method and microscope device |
| JP2009036969A (en) * | 2007-08-01 | 2009-02-19 | Nikon Corp | Cover glass, slide glass, preparation, observation method, and microscopic device |
| CN101493398A (en) * | 2009-03-04 | 2009-07-29 | 大庆油田有限责任公司 | Emulsified crude oil emulsion laser co-focussing analytical method |
| CN102499235A (en) * | 2011-11-08 | 2012-06-20 | 哈尔滨师范大学 | Preparation method of diatom sample |
Non-Patent Citations (1)
| Title |
|---|
| 彭亮，胡韧: "《水库蓝藻水华监测与管理》", 1 August 2011 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105532651A (en) * | 2015-12-15 | 2016-05-04 | 上海师范大学 | Method for making permanent glass slide specimen of bryophyte |
| CN105532651B (en) * | 2015-12-15 | 2018-02-09 | 上海师范大学 | A kind of bryophyte permanent slide Slide processing |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
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| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20130130 |