CN102897866B - Biological fermentation liquor dewatering device and using method thereof - Google Patents
Biological fermentation liquor dewatering device and using method thereof Download PDFInfo
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- CN102897866B CN102897866B CN 201210378329 CN201210378329A CN102897866B CN 102897866 B CN102897866 B CN 102897866B CN 201210378329 CN201210378329 CN 201210378329 CN 201210378329 A CN201210378329 A CN 201210378329A CN 102897866 B CN102897866 B CN 102897866B
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Abstract
The invention relates to a biological fermentation liquor dewatering device. The biological fermentation liquor dewatering device comprises a fermentation liquor storage tank, an eluate storage tank, a fermentation liquor pump, an eluate pump, an adsorber, a compressed air steel cylinder, a first electromagnetic valve, a second electromagnetic valve, a detector, a dividing valve, a product storage tank, a waste liquor storage tank and a connecting pipeline, and is further provided with a software control system for controlling the fermentation liquor pump, the eluate pump, the first electromagnetic valve, the second electromagnetic valve and the detector. The invention further relates to a using method of a biological fermentation liquor concentration device, and the method comprises the steps of adsorbing target products, removing residual fermentation liquor and eluting the target products, wherein the operation is performed in a circulating manner till the fermentation liquor is used up. The biological fermentation liquor dewatering device disclosed by the invention has the beneficial effects of high efficiency, continuity, flexibility in use, simple operation, high controllability and high popularization value.
Description
Technical field
The present invention relates to a kind of bio-fermented liquid de-watering apparatus and using method thereof.
Background technology
Bioengineering field develop rapidly in recent years, various biological products have been brought into play important effect at aspects such as ensureing human body health.Fermentation is the basic operation in bioengineering, and sweat completes in the aqueous solution, and target product in fermentation liquor content is often less, in order to obtain target product, need to be concentrated to zymotic fluid, and remove a large amount of water of the inside.The methods such as general normal employing heating evaporation, freeze drying, but these operations need expend mass energy, have increased production cost.
In addition, in the production process of biological products, reversed-phase preparative chromatography separates, ion-exchange chromatography separates is also to separate and concentrated means commonly used, but contains equally a large amount of water in the target product solution that adopts these methods to prepare, follow-up also the needs its concentrated dewatering.
Therefore, be badly in need of a kind ofly can solving this problem to the device cheaply of volume fermented liquid continuous dewatering esterase.
Summary of the invention
The technical problem to be solved in the present invention is: based on the problems referred to above, the invention provides a kind of bio-fermented liquid de-watering apparatus and using method thereof, this device is based on the powerful reservation on special adsorbent of the target products such as protein, polypeptide, polysaccharide in zymotic fluid, by the method for strong eluant, eluent wash-out, realize the quick continuous dewatering esterase process of volume fermented liquid.
The present invention solves the technical scheme that its technical problem adopts: a kind of bio-fermented liquid de-watering apparatus, formed by the fermentation liquid pump of zymotic fluid storage tank, eluent storage tank, controlled fermentation liquid storage tank, wash-out liquid pump, absorber, tank of compressed air, the first magnetic valve, the second magnetic valve, detector, flow divider, product storage tank, waste tank and the connecting line of control eluent storage tank, also be provided with the software control system of controlled fermentation liquid pump, wash-out liquid pump, the first magnetic valve, the second magnetic valve and detector.
The first magnetic valve connects fermentation liquid pump, wash-out liquid pump, absorber and tank of compressed air, and the other end of absorber connects flow divider, and flow divider is connection detector and the second magnetic valve also, and the second magnetic valve also connects product storage tank, waste tank and zymotic fluid storage tank.
The split ratio of flow divider is adjusted according to the detector detection range, reduces as far as possible and leads to the detector sample size.
The zymotic fluid flow rate pump is specifically adjusted according to the device system, prevents because flow velocity is too fast or excessively affect slowly the adsorbent absorption behavior.
Absorber is inner adsorbing sphere or the post that is filled with adsorbent, the adsorbent loaded in adsorbing sphere or post is anti-phase C18, C8, C4 filler or macroreticular resin, utilize non-specific adsorption effect between the target products such as materials and protein, polypeptide, polysaccharide, it is retained in the above preferably.
Eluting solvent is organic solvent methyl alcohol, acetonitrile, isopropyl alcohol or its aqueous solution or its haline water solution.Utilize the strong eluting power of this kind solvent, the target product adsorbed on wash-out adsorbing sphere or post, and remove eluting solvent under low-temperature evaporation or lyophilisation condition, thus obtain purer target product.
A kind of using method of bio-fermented liquid de-watering apparatus, its implementation process is:
(1) by adjusting the first magnetic valve and the second magnetic valve, make zymotic fluid storage tank, fermentation liquid pump, absorber and waste tank form path, open the fermentation liquid pump, zymotic fluid is with certain flow rate process absorber, simultaneously in absorber with adsorbent generation absorption behavior, flow out and shunted by certain split ratio by flow divider from absorber, make few partially liq enter detector and detect (on detectability), the most of waste tank that flows into of the liquid after shunting.Observation detector efflux composition changes, and when finding that there is the target product outflow, illustrates that absorber reaches capacity, and now software control system meets with a response, and sends and walks instruction.
(2) close the fermentation liquid pump, adjusting the first magnetic valve and the second magnetic valve makes compressed gas cylinder, absorber, zymotic fluid storage tank form path, gas in compressed gas cylinder blows back the remaining zymotic fluid in absorber in the zymotic fluid storage tank with certain flow velocity, simultaneously by observing detector, monitor remaining zymotic fluid and blow out progress, while in detector, air peak occurring, illustrate that the residual solution in absorber is blown back in the zymotic fluid storage tank fully, now software control system meets with a response, and sends next step operational order.
(3) stop passing into gas, adjust the first magnetic valve and the second magnetic valve, make the eluent storage tank, absorber, the product storage tank forms path, open the wash-out liquid pump, eluent in the eluent storage tank is with certain flow velocity process absorber, the target product retained on adsorbent in absorber is eluted, with eluent, enter together in the product storage tank, simultaneously by detector monitors wash-out progress, when in detector, no signal responds, illustrate that the target product wash-out is complete, now pass through software control system, send next step operational order, return to the operation of step (1).
(4) repeating step (1)～(3), realize the continuous collection of zymotic fluid continuous dewatering esterase and target product.
(5) liquid in the product storage tank is evaporated or freeze-drying at low temperatures, thereby obtain purer target product.
The invention has the beneficial effects as follows: 1. high-efficiency and continuous: dewater by circulation absorption, greatly increased the continuity of thickening efficiency and process, reduced the loss amount of target product simultaneously; 2. use flexibly: can choose suitable adsorbent and eluant, eluent according to the value volume and range of product of bio-fermented liquid, optimize removal process; 3. simple to operate, controllability is high: by computer software control, greatly increased the operability of device; 4. promotional value is high: this device is not only concentrated for dewatering of bio-fermented liquid, can also be applied to enrichment and the collection of other large volumes, low concentration organic sample.
The accompanying drawing explanation
Below in conjunction with accompanying drawing, the present invention is further described.
Fig. 1 is frame diagram of the present invention.
Wherein: 1. zymotic fluid storage tank, 2. eluent storage tank, 3. fermentation liquid pump, 4. wash-out liquid pump, 5. absorber, 6. tank of compressed air, 7. the first magnetic valve, 8. the second magnetic valve, 9. detector, 10. flow divider, 11. product storage tanks, 12. software control systems, 13. waste tank.
The specific embodiment
The invention will be further described in conjunction with specific embodiments now, and following examples are intended to illustrate the present invention rather than limitation of the invention further.
A kind of bio-fermented liquid de-watering apparatus as shown in Figure 1, formed by zymotic fluid storage tank 1, eluent storage tank 2, fermentation liquid pump 3, wash-out liquid pump 4, absorber 5, tank of compressed air 6, the first magnetic valve 7, the second magnetic valve 8, detector 9, flow divider 10, product storage tank 11, waste tank 13 and connecting line, also be provided with the software control system 12 of controlled fermentation liquid pump 3, wash-out liquid pump 4, the first magnetic valve 7, the second magnetic valve 8 and detector 9.The first magnetic valve 7 connects fermentation liquid pump 3, wash-out liquid pump 4, absorber 5 and tank of compressed air 6, the other end of absorber 5 connects flow divider 10, connection detector 9 gone back by flow divider 10 and the second magnetic valve 8, the second magnetic valves 8 also connect product storage tank 11, waste tank 13 and zymotic fluid storage tank 1.
Embodiment:
Adjusting the first magnetic valve 7 and the second magnetic valve 8 makes zymotic fluid holding vessel 1, fermentation liquid pump 3, absorber 5 and waste tank 13 form path, open fermentation liquid pump 3, the 1000ml zymotic fluid is with the flow velocity process absorber 5 of 5ml/min, and the efflux flowed out from adsorption column 5 is shunted with the split ratio of 1:50 through flow divider 10.When chromatographic peak appears in detector 9, send instruction by software control system 12, close fermentation liquid pump 3, adjust the first magnetic valve 7 and the second magnetic valve 8 and make compressed gas cylinder 6, absorber 5, zymotic fluid holding vessel 1 form path, the gas in compressed gas cylinder 6 blows back the remaining zymotic fluid in absorber 5 in zymotic fluid holding vessel 1 with the flow velocity of 3ml/min.While in detector 9, sharp-pointed air peak occurring, send instruction by software control system 12, stop passing into gas, adjust the first magnetic valve 7 and the second magnetic valve 8 simultaneously, make eluent storage tank 2, absorber 5, product storage tank 11 form path, open wash-out liquid pump 4, the eluent acetonitrile is with the flow velocity process absorber 5 of 5ml/min.When no signal occurs in detector 9, by software control system 12, send instruction, repeat above all operations, until in zymotic fluid fluid reservoir 1, the 1000ml zymotic fluid exhausts.Fluid sample in product storage tank 11 is evaporated to acetonitrile, thereby obtain the anhydrous target product of 267g.
In the zymotic fluid concentration process, adopt the non-specific adsorption of adsorbent and target product to reach the concentrated effect that dewaters, in actual applications, can also utilize the specific adsorption effect to carry out enrichment continuously and collection to the organic sample of other large volumes, low concentration.
The above-mentioned foundation desirable embodiment of the present invention of take is enlightenment, and by above-mentioned description, the relevant staff can, in the scope that does not depart from this invention technological thought, carry out various change and modification fully.The technical scope of this invention is not limited to the content on specification, must determine its technical scope according to the claim scope.
Claims (6)
1. a bio-fermented liquid de-watering apparatus, it is characterized in that: by zymotic fluid storage tank (1), eluent storage tank (2), the fermentation liquid pump (3) of controlled fermentation liquid storage tank (1), control the wash-out liquid pump (4) of eluent storage tank (2), absorber (5), tank of compressed air (6), the first magnetic valve (7), the second magnetic valve (8), detector (9), flow divider (10), product storage tank (11), waste tank (13) and connecting line form, also be provided with controlled fermentation liquid pump (3), wash-out liquid pump (4), the first magnetic valve (7), the software control system (12) of the second magnetic valve (8) and detector (9),
The first magnetic valve (7) is connected with absorber (5) pipeline, the first magnetic valve (7) separates four streams and is connected by pipeline with absorber (5), tank of compressed air (6), fermentation liquid pump (3), wash-out liquid pump (4) respectively, zymotic fluid storage tank (1), fermentation liquid pump (3), absorber (5), tank of compressed air (6) form the loading stream, and eluent storage tank (2), wash-out liquid pump (4), absorber (5) form the wash-out stream;
Flow divider (10) is connected with absorber (5) pipeline, and flow divider (10) is connected with detector (9) with the second magnetic valve (8) respectively by pipeline;
The second magnetic valve (8) separates four streams and is connected by pipeline with flow divider (10), product storage tank (11), waste tank (13) and zymotic fluid storage tank (1) respectively;
The detection signal that software control system (12) obtains based on detector (9) is controlled the action of the first magnetic valve (7), the second magnetic valve (8), and the open and close of fermentation liquid pump (3) and wash-out liquid pump (4).
2. a kind of bio-fermented liquid de-watering apparatus according to claim 1 is characterized in that: described absorber (5) is inner adsorbing sphere or the post that is filled with adsorbent.
3. the using method of a bio-fermented liquid de-watering apparatus as claimed in claim 1, it is characterized in that: concrete operation step comprises:
A. regulate the position that the first magnetic valve (7) is communicated with absorber (5) in the liquid pump (3) that makes to ferment, the position of the second magnetic valve (8) in absorber (5) is communicated with waste tank (13), open fermentation liquid pump (3), zymotic fluid in zymotic fluid storage tank (1) is liquid pump (3) by fermentation, the first magnetic valve (7) flows into absorber (5), zymotic fluid flows out by flow divider (10) and shunts from absorber (5), it is detected that part enters detector (9), another part zymotic fluid after shunting flows into waste tank (13) through the second magnetic valve (8),
B. observed the composition variation of efflux by detector (9), when having target product to flow out, information is fed back to software control system (12), send instruction;
C. the liquid pump (3) that ferments stops, the first magnetic valve (7) is switched to the position that tank of compressed air (6) is communicated with absorber (5), the second magnetic valve (8) is switched to the position that absorber (5) is communicated with zymotic fluid storage tank (1), gas in tank of compressed air (6) blows back zymotic fluid storage tank (1) through the first magnetic valve (7) by the remaining zymotic fluid in absorber (5), observe the progress that blows out of remaining zymotic fluid by detector (9), when in detector (9), air peak occurring, information is fed back to software control system (12), enter next step operation,
D. the first magnetic valve (7) is placed in to the position that wash-out liquid pump (4) is communicated with absorber (5), the second magnetic valve (8) is placed in the position that absorber (5) is communicated with product storage tank (11), open wash-out liquid pump (4), eluent in eluent storage tank (2) enters in absorber (5) through wash-out liquid pump (4), the first magnetic valve (7), target product is washed out, and the solution after washing out enters into product storage tank (11) through the second magnetic valve (8) and preserves;
E. observe by detector (9) progress that washes out, after target product is by complete wash-out, information is fed back to software control system (12), turn back to the operation of step a;
F. repeating step a～e, until the zymotic fluid in zymotic fluid storage tank (1) is finished.
4. using method according to claim 3 is characterized in that: the solution in described product storage tank (11), by heating evaporation or freeze-drying, obtains target product.
5. using method according to claim 3 is characterized in that: the adsorbent of loading in described absorber (5) is anti-phase C18, C8, C4 filler or macroreticular resin.
6. using method according to claim 3, it is characterized in that: described eluent is methyl alcohol, acetonitrile, isopropyl alcohol or its aqueous solution or its haline water solution.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210378329 CN102897866B (en) | 2012-09-29 | 2012-09-29 | Biological fermentation liquor dewatering device and using method thereof |
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| CN 201210378329 CN102897866B (en) | 2012-09-29 | 2012-09-29 | Biological fermentation liquor dewatering device and using method thereof |
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| CN102897866A CN102897866A (en) | 2013-01-30 |
| CN102897866B true CN102897866B (en) | 2013-12-18 |
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| JP2003300035A (en) * | 2002-04-04 | 2003-10-21 | Livestock Industry's Environmental Improvement Organization | Methane fermentation treatment system and denitrification method of its digested liquid |
| CN1159447C (en) * | 2002-10-22 | 2004-07-28 | 华宝香化科技发展（上海）有限公司 | Microbe fermenting process of producing perfume phenylethanol |
| CN202822865U (en) * | 2012-09-29 | 2013-03-27 | 常州博世伟业生物科技有限公司 | Water removal device for biological fermentation liquor |
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Granted publication date: 20131218 Termination date: 20200929 |