CN102897866A - Biological fermentation liquor dewatering device and using method thereof - Google Patents
Biological fermentation liquor dewatering device and using method thereof Download PDFInfo
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- CN102897866A CN102897866A CN2012103783292A CN201210378329A CN102897866A CN 102897866 A CN102897866 A CN 102897866A CN 2012103783292 A CN2012103783292 A CN 2012103783292A CN 201210378329 A CN201210378329 A CN 201210378329A CN 102897866 A CN102897866 A CN 102897866A
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Abstract
The invention relates to a biological fermentation liquor dewatering device. The biological fermentation liquor dewatering device comprises a fermentation liquor storage tank, an eluate storage tank, a fermentation liquor pump, an eluate pump, an adsorber, a compressed air steel cylinder, a first electromagnetic valve, a second electromagnetic valve, a detector, a dividing valve, a product storage tank, a waste liquor storage tank and a connecting pipeline, and is further provided with a software control system for controlling the fermentation liquor pump, the eluate pump, the first electromagnetic valve, the second electromagnetic valve and the detector. The invention further relates to a using method of a biological fermentation liquor concentration device, and the method comprises the steps of adsorbing target products, removing residual fermentation liquor and eluting the target products, wherein the operation is performed in a circulating manner till the fermentation liquor is used up. The biological fermentation liquor dewatering device disclosed by the invention has the beneficial effects of high efficiency, continuity, flexibility in use, simple operation, high controllability and high popularization value.
Description
Technical field
The present invention relates to a kind of bio-fermented liquid de-watering apparatus and using method thereof.
Background technology
In recent years bioengineering field develop rapidly, various biological products have been brought into play important effect at aspects such as ensureing human body health.Fermentation is the elementary operation in the biotechnology, and fermenting process is finished in the aqueous solution, and target product in fermentation liquor content is often less, in order to obtain target product, need to be concentrated to fermented liquid, and remove a large amount of water of the inside.The methods such as general normal employing heating evaporation, lyophilize, but these operations need expend mass energy, have increased production cost.
In addition, in the production process of biological products, it also is to separate and concentrated means commonly used that reversed-phase preparative chromatography separation, ion-exchange chromatography separate, but contains equally a large amount of water in the target product solution that adopts these methods to prepare, follow-up also the needs its concentrated dewatering.
Therefore, urgent need is a kind of can solve this problem to the cheaply device of volume fermented liquid continuous dewatering esterase.
Summary of the invention
The technical problem to be solved in the present invention is: based on the problems referred to above, the invention provides a kind of bio-fermented liquid de-watering apparatus and using method thereof, this device is based on the powerful reservation on special adsorbent of the target products such as protein, polypeptide, polysaccharide in the fermented liquid, by the method for strong eluent wash-out, realize the quick continuous dewatering esterase process of volume fermented liquid.
The present invention solves the technical scheme that its technical problem adopts: a kind of bio-fermented liquid de-watering apparatus, consisted of by the fermentation liquid pump of fermented liquid storage tank, elutriant storage tank, controlled fermentation liquid storage tank, wash-out liquid pump, adsorber, tank of compressed air, the first magnetic valve, the second magnetic valve, detector, diverting valve, product storage tank, waste tank and the connecting pipeline of control elutriant storage tank, also be provided with the software control system of controlled fermentation liquid pump, wash-out liquid pump, the first magnetic valve, the second magnetic valve and detector.
The first magnetic valve connects fermentation liquid pump, wash-out liquid pump, adsorber and tank of compressed air, and the other end of adsorber connects diverting valve, and diverting valve is connection detector and the second magnetic valve also, and the second magnetic valve also connects product storage tank, waste tank and fermented liquid storage tank.
The splitting ratio of diverting valve is adjusted according to the detector sensing range, reduces as far as possible to lead to the detector sample size.
The fermented liquid flow rate pump is specifically adjusted according to the device system, and preventing too fast because of flow velocity or crossing affects the sorbent material absorption behavior slowly.
Adsorber is inner adsorbing sphere or the post that is filled with sorbent material, the sorbent material that loads in adsorbing sphere or the post is anti-phase C18, C8, C4 filler or macroporous resin, utilize non-specific adsorption effect between the target products such as materials and protein, polypeptide, polysaccharide, it is kept in the above preferably.
Eluting solvent is organic solvent methyl alcohol, acetonitrile, Virahol or its aqueous solution or its haline water solution.Utilize the strong elutive power of this kind solvent, the target product that adsorbs on wash-out adsorbing sphere or the post, and under low-temperature evaporation or lyophilisation condition, remove eluting solvent, thus obtain purer target product.
A kind of using method of bio-fermented liquid de-watering apparatus, its implementation process is:
(1) passes through to adjust the first magnetic valve and the second magnetic valve so that fermented liquid storage tank, fermentation liquid pump, adsorber and waste tank form path, open the fermentation liquid pump, fermented liquid is with certain flow rate process adsorber, simultaneously in adsorber with sorbent material generation absorption behavior, shunt by certain splitting ratio by diverting valve from the adsorber outflow, so that entering detector, few partially liq detects (on detectability), the most of waste tank that flows into of the liquid after the shunting.Observation detector effluent liquid composition changes, and when finding to have target product to flow out, illustrates that adsorber reaches capacity, and this moment, software control system met with a response, and sent and went on foot instruction.
(2) close the fermentation liquid pump, adjust the first magnetic valve and the second magnetic valve so that compressed gas cylinder, adsorber, fermented liquid storage tank form path, gas in the compressed gas cylinder blows back the remaining fermented liquid in the adsorber in the fermented liquid storage tank with certain flow velocity, simultaneously by observing detector, monitor remaining fermented liquid and blow out progress, when air peak occurring in the detector, illustrate that the residual solution in the adsorber is blown back in the fermented liquid storage tank fully, this moment, software control system met with a response, and sent next step operational order.
(3) stop to pass into gas, adjust the first magnetic valve and the second magnetic valve, so that elutriant storage tank, adsorber, the product storage tank forms path, open the wash-out liquid pump, elutriant in the elutriant storage tank is with certain flow velocity process adsorber, the target product that keeps in the agent of adsorber internal adsorption is eluted, enter together in the product storage tank with elutriant, simultaneously by detector monitors wash-out progress, when no signal in the detector responds, illustrate that the target product wash-out is complete, pass through software control system this moment, send next step operational order, return the operation of step (1).
(4) the continuous collection of fermented liquid continuous dewatering esterase and target product is realized in repeating step (1)～(3).
(5) liquid in the product storage tank is evaporated or freeze-drying at low temperatures, thereby obtain purer target product.
The invention has the beneficial effects as follows: 1. high-efficiency and continuous: dewater by circulation absorption, greatly increased the continuity of thickening efficiency and process, reduced simultaneously the loss amount of target product; 2. use flexibly: can choose suitable sorbent material and eluent according to the value volume and range of product of bio-fermented liquid, optimize removal process; 3. simple to operate, controllability is high: by computer software control, greatly increased the operability of device; 4. promotional value is high: it is concentrated that this device not only is used for dewatering of bio-fermented liquid, can also be applied to enrichment and the collection of other large volumes, lower concentration organic sample.
Description of drawings
The present invention is further described below in conjunction with accompanying drawing.
Fig. 1 is frame diagram of the present invention.
Wherein: 1. fermented liquid storage tank, 2. elutriant storage tank, 3. fermentation liquid pump, 4. wash-out liquid pump, 5. adsorber, 6. tank of compressed air, 7. the first magnetic valve, 8. the second magnetic valve, 9. detector, 10. diverting valve, 11. product storage tanks, 12. software control systems, 13. waste tank.
Embodiment
The invention will be further described in conjunction with specific embodiments now, and following examples are intended to illustrate the present invention rather than limitation of the invention further.
A kind of bio-fermented liquid de-watering apparatus as shown in Figure 1, consisted of by fermented liquid storage tank 1, elutriant storage tank 2, fermentation liquid pump 3, wash-out liquid pump 4, adsorber 5, tank of compressed air 6, the first magnetic valve 7, the second magnetic valve 8, detector 9, diverting valve 10, product storage tank 11, waste tank 13 and connecting pipeline, also be provided with the software control system 12 of controlled fermentation liquid pump 3, wash-out liquid pump 4, the first magnetic valve 7, the second magnetic valve 8 and detector 9.The first magnetic valve 7 connects fermentation liquid pump 3, wash-out liquid pump 4, adsorber 5 and tank of compressed air 6, the other end of adsorber 5 connects diverting valve 10, connection detector 9 gone back by diverting valve 10 and the second magnetic valve 8, the second magnetic valves 8 also connect product storage tank 11, waste tank 13 and fermented liquid storage tank 1.
Embodiment:
Adjust the first magnetic valve 7 and the second magnetic valve 8 so that fermented liquid hold-up vessel 1, fermentation liquid pump 3, adsorber 5 and waste tank 13 form path, open fermentation liquid pump 3, the 1000ml fermented liquid is shunted with the splitting ratio of 1:50 through diverting valve 10 from the effluent liquid that adsorption column 5 flows out with the flow velocity process adsorber 5 of 5ml/min.When chromatographic peak appears in detector 9, send instruction by software control system 12, close fermentation liquid pump 3, adjust the first magnetic valve 7 and the second magnetic valve 8 so that compressed gas cylinder 6, adsorber 5, fermented liquid hold-up vessel 1 forms path, the gas in the compressed gas cylinder 6 blows back the remaining fermented liquids in the adsorber 5 in the fermented liquid hold-up vessel 1 with the flow velocity of 3ml/min.When sharp-pointed air peak occurring in the detector 9, send instruction by software control system 12, stop to pass into gas, adjust simultaneously the first magnetic valve 7 and the second magnetic valve 8, so that elutriant storage tank 2, adsorber 5, product storage tank 11 forms paths, open wash-out liquid pump 4, the elutriant acetonitrile is with the flow velocity process adsorber 5 of 5ml/min.When no signal occurs in the detector 9, send instruction by software control system 12, repeat above all operations, until the 1000ml fermented liquid exhausts in the fermented liquid container for storing liquid 1.Liquid sample in the product storage tank 11 is evaporated acetonitrile, thereby obtain the anhydrous target product of 267g.
Adopt the non-specific adsorption of sorbent material and target product to reach the concentrated effect that dewaters in the fermented liquid concentration process, in actual applications, can also utilize the specific adsorption effect that the organic sample of other large volumes, lower concentration is carried out continuously enrichment and collection.
Take above-mentioned foundation desirable embodiment of the present invention as enlightenment, by above-mentioned description, the relevant staff can in the scope that does not depart from this invention technological thought, carry out various change and modification fully.The technical scope of this invention is not limited to the content on the specification sheets, must determine its technical scope according to the claim scope.
Claims (6)
1. bio-fermented liquid de-watering apparatus, it is characterized in that: by fermented liquid storage tank (1), elutriant storage tank (2), the fermentation liquid pump (3) of controlled fermentation liquid storage tank (1), the wash-out liquid pump (4) of control elutriant storage tank (2), adsorber (5), tank of compressed air (6), the first magnetic valve (7), the second magnetic valve (8), detector (9), diverting valve (10), product storage tank (11), waste tank (13) and connecting pipeline consist of, and also are provided with controlled fermentation liquid pump (3), wash-out liquid pump (4), the first magnetic valve (7), the software control system (12) of the second magnetic valve (8) and detector (9).
2. a kind of bio-fermented liquid de-watering apparatus according to claim 1 is characterized in that: described adsorber (5) is inner adsorbing sphere or the post that is filled with sorbent material.
3. the using method of a bio-fermented liquid de-watering apparatus, it is characterized in that: concrete operation step comprises:
A. regulate the first magnetic valve (7) and be in the position that fermentation liquid pump (3) is communicated with adsorber (5), the second magnetic valve (8) is in the position that adsorber (5) is communicated with waste tank (13), open fermentation pump (3), fermented liquid in the fermented liquid storage tank (1) is liquid pump (3) by fermentation, the first magnetic valve (7) flows into adsorber (5), fermented liquid flows out by diverting valve (10) from adsorber (5) to be shunted, it is detected that part enters detector (9), and another part fermented liquid after the shunting flows into waste tank (13) through the second magnetic valve (8);
B. observed the composition variation of effluent liquid by detector (9), when having target product to flow out, information is fed back to software control system (12), send instruction;
C. the liquid pump (3) that ferments stops, the first magnetic valve (7) is switched to the position that tank of compressed air (6) is communicated with adsorber (5), the second magnetic valve (8) is switched to the position that adsorber (5) is communicated with fermented liquid storage tank (1), gas in the tank of compressed air (6) blows back fermented liquid storage tank (1) through the first magnetic valve (7) with the remaining fermented liquid in the adsorber (5), observe the progress that blows out of remaining fermented liquid by detector (9), when in detector (9), air peak occurring, information is fed back to software control system (12), enter next step operation;
D. the position that places wash-out liquid pump (4) to be communicated with adsorber (5) the first magnetic valve (7), the position that the second magnetic valve (8) places adsorber (5) to be communicated with product storage tank (11), open wash-out liquid pump (4), elutriant in the elutriant storage tank (2) enters in the adsorber (5) through wash-out liquid pump (4), the first magnetic valve (7), target product is washed out, and the solution after washing out enters into product storage tank (11) through the second magnetic valve (8) and preserves;
E. observe the progress that washes out by detector (9), after target product is by complete wash-out, information is fed back to software control system (12), turn back to the operation of step a;
F. repeating step a～e is until the fermented liquid in the fermented liquid storage tank (1) is finished.
4. according to claim 3 a kind of using method of bio-fermented liquid de-watering apparatus, it is characterized in that: the solution in the described product storage tank (11) obtains target product by heating evaporation or freeze-drying.
5. according to claim 3 a kind of using method of bio-fermented liquid de-watering apparatus, it is characterized in that: the sorbent material of loading in the described adsorber (5) is anti-phase C18, C8, C4 filler or macroporous resin.
6. according to claim 3 a kind of using method of bio-fermented liquid de-watering apparatus, it is characterized in that: described elutriant is methyl alcohol, acetonitrile, Virahol or its aqueous solution or its haline water solution.
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| CN 201210378329 CN102897866B (en) | 2012-09-29 | 2012-09-29 | Biological fermentation liquor dewatering device and using method thereof |
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| CN 201210378329 CN102897866B (en) | 2012-09-29 | 2012-09-29 | Biological fermentation liquor dewatering device and using method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106769362A (en) * | 2017-02-08 | 2017-05-31 | 中国环境科学研究院 | A kind of full-automatic adsorption experimental apparatus |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1403581A (en) * | 2002-10-22 | 2003-03-19 | 华宝香化科技发展（上海）有限公司 | Microbe fermenting process of producing perfume phenylethanol |
| JP2003300035A (en) * | 2002-04-04 | 2003-10-21 | Livestock Industry's Environmental Improvement Organization | Methane fermentation treatment system and denitrification method of its digested liquid |
| CN202822865U (en) * | 2012-09-29 | 2013-03-27 | 常州博世伟业生物科技有限公司 | Water removal device for biological fermentation liquor |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003300035A (en) * | 2002-04-04 | 2003-10-21 | Livestock Industry's Environmental Improvement Organization | Methane fermentation treatment system and denitrification method of its digested liquid |
| CN1403581A (en) * | 2002-10-22 | 2003-03-19 | 华宝香化科技发展（上海）有限公司 | Microbe fermenting process of producing perfume phenylethanol |
| CN202822865U (en) * | 2012-09-29 | 2013-03-27 | 常州博世伟业生物科技有限公司 | Water removal device for biological fermentation liquor |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106769362A (en) * | 2017-02-08 | 2017-05-31 | 中国环境科学研究院 | A kind of full-automatic adsorption experimental apparatus |
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