CN102885710A - Sodium alga acid/chitosan/collagen composite micro-ball with active components and preparation method of composite micro-ball - Google Patents
Sodium alga acid/chitosan/collagen composite micro-ball with active components and preparation method of composite micro-ball Download PDFInfo
- Publication number
- CN102885710A CN102885710A CN2012104006116A CN201210400611A CN102885710A CN 102885710 A CN102885710 A CN 102885710A CN 2012104006116 A CN2012104006116 A CN 2012104006116A CN 201210400611 A CN201210400611 A CN 201210400611A CN 102885710 A CN102885710 A CN 102885710A
- Authority
- CN
- China
- Prior art keywords
- acid
- chitosan
- microsphere
- alginate
- ball
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Abstract
The invention discloses a micro-ball with a plurality of cosmetic active components, which is prepared by using collagen, sodium alga acid and chitosan as carrier materials through the following steps of: dissolving collagen in acid medium, mixing with sodium alga acid and active components to form a uniform colloidal solution; enabling the mixture to enter a divalent positive ion solution by using an injector in a liquid drop form, forming a primary micro-ball under the action of gel; arranging the micro-ball in a chitosan solution, further curing; and washing the micro-ball by using de-ionized water to recombine the collagen in the micro-ball, and finally, forming the sodium alga acid/chitosan/collagen composite micro-ball. The micro-ball prepared by using the method provided by the invention has a smooth and flat surface, excellent monodispersity, uniform particle size distribution and fast swelling property, and belongs to a novel cosmetic active component carrier system; and the free-dried micro-ball can be used together with solvents, in this way, the effect of the common action of multi-component various-function cosmetic active components is achieved.
Description
Technical field
The present invention relates to a kind of novel cosmetic load system, be specifically related to a kind of alginate/chitosan of carrying active composition/collagen composite microsphere and preparation method thereof, belong to the cosmetics preparing technical field.
Background technology
Arbutin, fruit acid, anisic acid etc. are current popular more safely and effectively water solublity whitening raw materials, can reduce Skin pigmentation, dispelling stain and freckle.Ceramide is a kind of in the glycosphingolipid, is a kind of water-soluble grease metallic substance, can improve cuticular water conservation and barrier function, solves xerosis cutis, wrinkle, coarse phenomenon.Somatomedin is the cytokine with stimulating cellular growth activity, and result of study proves: somatomedin reduces with advancing age gradually, and human body shows various senile symptoms.Class cell growth factor take epithelical cell growth factor (EGF) and fibroblast growth factor (FGF) as representative has significantly cosmetic result, can solve skin senilism, sallow, wrinkle, the phenomenon such as lax, has the deep layer repair.Chinese patent CN101732169A report is with the prescription of arbutin for the function skin care liquid, and Japanese Mercian Corp discloses the patent (CN101583345) of ceramide synthesis accelerators as cosmetics and skin preparations for extenal use.Chinese patent CN102406556A discloses a kind of bioactive cosmetic composition of epidermal growth factor that helps to keep, and the main component of said composition is epidermal growth factor, Hy drolyzed Skin Powder, hyaluronate sodium etc.Chinese patent CN101548935 discloses the purposes of a kind of acid fibroblast growth factor in the preparation cosmetics, and its production technology also is provided simultaneously.The prior art self stability is not good enough, is subjected to the easy inactivation of external interference, for example decomposes easily under light and the inappropriate condition of surrounding, and also easily oxidation lacks the stability of storage in air, so has been subject to certain restriction in cosmetic applications.
Sodium alginate (C
6H
7O
8Na) n mainly is comprised of the sodium salt of alginic acid, the copolymer that is relied on Isosorbide-5-Nitrae-glycosidic bond to be connected and be comprised of GM, MM and the GG fragment of different proportion by a-L-mannuronic acid (M unit) and b-D-guluronic acid (G unit).Sodium alginate has just been taken in American Pharmacopeia as far back as 1938, and its gentle sol-gel process, good biocompatibility make sodium alginate be suitable for microcapsule carrier as release or embedding medicinal, albumen and cell.Chitosan; chemical name is β-(1; 4)-2-amino-2-deoxidation-D-glucosan; it is the product of chitin N-deacetylation; it also is unique a kind of alkaline aminopolysaccharide that biosphere exists; water insoluble but can be dissolved in low-concentration organic acid or mineral acid, the microsphere of preparation has the control release take chitosan as carrier, improves many-sided advantages such as medicine stability.
At present, use the method that activated protein or medicine microspheres are carried in maximum preparations that solvent evaporated method is arranged, for example patent CN1463696 has reported the Poly-dl-lactide-b-polyethylene glycol microsphere of two emulsion system solvent evaporated method preparation year protein medicaments, and US Patent No. 5288502 has been reported the microsphere that utilizes the emulsion solvent evaporation technique preparation to carry polypeptide and albumen; Patent CN1398584A has reported and has utilized the standby bFGF-PLGA sustained-release micro-spheres of emulsion dispersion legal system; US Patent No. 2007275082 has been reported the method for utilizing spray drying method Bidirectional nozzle bag medicine carrying thing, said method generally all will be used organic solvent or cross-linking agent, it has the following disadvantages, the one, the easy stability of destroying the active substance that coats, the 2nd, remaining in easily affects the microsphere performance in the microsphere, the 3rd, the carrier of using is to synthesize family macromolecule and semi-synthetic family macromolecule as main, and is not good enough in the biocompatibility of human body.
Summary of the invention
The object of the present invention is to provide a kind of good biocompatibility, not with an organic solvent, cross-linking agent and emulsifying agent, load does not have the alginate/chitosan of multiple efficacies cosmetic active ingredient/collagen composite microsphere.
Another object of the present invention is to provide that a kind of technique is simple, cost is low, gentle environmental protection, the load of the active ingredient good stability for preparing has the alginate/chitosan of multiple efficacies cosmetic active ingredient/collagen composite method for preparing microsphere.
Implementation procedure of the present invention is as follows:
The alginate/chitosan of carrying active composition/collagen composite microsphere, obtained by sodium alginate, chitosan, Ι Collagen Type VI and active fraction preparation, wherein sodium alginate, chitosan, Ι Collagen Type VI and active component weight ratio are 3 ~ 10:2 ~ 9:1 ~ 3:2 ~ 5 in the complex microsphere, described active component is selected from whitening active component, moisturizing active component ceramide and the activity of fighting against senium composition cell growth factor at least two kinds, and the whitening active component is selected from arbutin, fruit acid, anisic acid, aloin.
The alginate/chitosan of above-mentioned carrying active composition/collagen composite method for preparing microsphere comprises the steps:
(1) the Ι Collagen Type VI is dissolved in the acid medium, adds sodium alginate and active component and mix formation homogeneous colloid solution, and remove bubble;
(2) above-mentioned colloid solution is squeezed to the elementary microsphere of formation in the bivalent cation solution;
(3) the elementary microsphere alginate/chitosan that places the chitosan acid solution further to solidify to obtain the carrying active composition/collagen composite microsphere.
In the above-mentioned steps (1), acid medium is organic or inorganic acid, and its pH is 1-4.Sodium alginate concentration is 0.1-10 % in the colloid solution for preparing, and Ι Collagen Type VI concentration is that 0.1-20 %(is preferably 0.5-10 %), activity component concentration is that 0.5-20 %(is preferably 1-10 %).
In the above-mentioned steps (2), bivalent cation is selected from Ba
2+, Ca
2+, Co
2+, Zn
2+, Mn
2+, Ni
2+, bivalent cation solution quality percent concentration is 0.1-10 %.Colloid solution is transferred in the glass syringe that is connected with the gas injection pump, regulates syringe pump speed mixture is squeezed in the bivalent cation solution, form elementary microsphere by gelatification.
In the above-mentioned steps (3), the chitosan acid solution is chitosan to be dissolved in formic acid, acetic acid, propanoic acid, lactic acid, malic acid, citric acid, ascorbic acid, oxalic acid, succinic acid, malonic acid, fatty acid, acetone acid, 1,3-propanedicarboxylic acid, tartaric acid, aspartic acid, salicylic acid or the glycollic acid solution obtain, the concentration of chitosan is 0.1-10 %, and the molecular weight of chitosan is 2-30 ten thousand.
The alginate/chitosan of above-mentioned carrying active composition/collagen composite microsphere can be used in the cosmetics, during use, the alginate/chitosan of carrying active composition/collagen composite microsphere is used after the swelling in solvent, described solvent is butanediol and hyaluronic aqueous solution, pH is 6.0-6.8, and wherein the concentration of butanediol is 2%.
The microsphere features smooth surface rounding that uses the inventive method to make, monodispersity is good, the particle size distribution homogeneous, has Fast-swelling character, its diameter Distribution coefficient can be controlled in 20%, diameter can be in 50-1500 μ m free adjustment, the swellbility in its 30s solvent＞200%.
The diameter Distribution coefficient=[(
dI-
d)
2/
N]
1/2/
d} * 100% (1)
Swellbility=[(
m t-
m 0)/
m 0] * 100% (2)
In the formula (1),
dI is the diameter of each microsphere,
dBe several average diameters, N is the microsphere quantity of calculated diameter, N＞200; In the formula (2),
m tThat microsphere is in t quality (g) constantly;
m 0It is the quality (g) of dry rear microsphere.
It is carrier that the present invention adopts sodium alginate, chitosan and the collagen of high-biocompatibility and reduced immunogenicity, by self assembly Electrostatic Absorption method the multiple different efficacies active component in the cosmetics is coated and to prepare alginate/chitosan/collagen composite microsphere, have the following advantages:
(1) do not add poisonous organic solvent, cross-linking agent and emulsifying agent etc. in the preparation method, therefore do not have residual noxious substance in the final microsphere, accomplished zero real pollution;
(2) a kind of microsphere supported simultaneously bag carries multiple different efficacies cosmetic active ingredient, has realized that same cosmetics have both the characteristics of different efficacies, has satisfied the demand of multifunction nursing articles for use;
(3) collocation of microsphere and solvent is used, and convenient and swift, instant joining, active component in time discharges, and has improved the availability of active component;
(4) preparation method technique is simple, cost is low, gentle environmental protection, and market prospect is wide.
Description of drawings
Fig. 1 microspheres flow chart.
Fig. 2 embodiment 1 microsphere photo.
The optical microscope photograph of the microsphere of Fig. 3 embodiment 2 preparations.
Fig. 4 embodiment 4 microsphere scanning electron microscope (SEM) photographs.
The microspherulite diameter scattergram of Fig. 5 embodiment 1-4, (A) the particle size distribution figure (the particle size distribution coefficient is 17.85%) of the microsphere of embodiment 1 preparation, (B) the particle size distribution figure (the particle size distribution coefficient is 18.23%) of the microsphere of embodiment 2 preparations, (C) the particle size distribution figure (the particle size distribution coefficient is 18.77%) of the microsphere of embodiment 3 preparations, (D) particle size distribution (figure particle size distribution coefficient is 19.05%) of the microsphere of embodiment 4 preparations.
The different indexs of Fig. 6 effect checking-test combinations matched group change the contrast trendgram.
The specific embodiment
The invention will be further described below in conjunction with embodiment, and concentration of the present invention is mass percent concentration, measuring instrument: scanning electron microscope JSM5800, laser particle analyzer COULTER LS200.
The present invention prepares the method for the alginate/chitosan that bag carries multiple cosmetic active ingredient/collagen composite microsphere, may further comprise the steps: purified abortive calfskin type i collagen is dissolved in the acid medium that pH value is 1-4, form homogeneous colloid solution 4-10 ℃ of lower the mixing mutually with sodium alginate and active component, the ultrasonic bubble of removing, wherein: the concentration range of collagen is 0.1-20 %, the concentration range of sodium alginate is 0.1-10%, the concentration range of cosmetic active ingredient (except cell growth factor) is 0.5-20 %, and the concentration range of cell growth factor is 0.1-10 μ g/ml.
Above-mentioned mixed colloidal solution is transferred in the glass syringe that is connected with the gas injection pump, gas in the syringe can be nitrogen, helium, argon, carbon dioxide and airborne any one, gas flow rate in the syringe can be controlled by syringe pump, can form like this microsphere of different size.
Mixture is pushed by the gas injection pump, and mixture overcomes viscous force and surface tension, enters the calcium chloride solution with droplet-like from pin hole, forms elementary microsphere by gelatification, and gelatification time 5-20min collects microsphere behind the sucking filtration.Cation in the solution can be Ba
2+, Ca
2+, Co
2+, Zn
2+, Mn
2+, Ni
2+In any one, usually select Ca
2+Carry out gelation, the concentration of bivalent cation is 0.1-10 % among the present invention.
Above-mentioned elementary microsphere is transferred to further curing in the chitosan solution that concentration is 0.1-10%, hardening time 5-30min, the pH value of chitosan solution is between 4.0-6.5, and the acid that is used for dissolving chitosan comprises at least a in formic acid, acetic acid, propanoic acid, lactic acid, malic acid, citric acid, ascorbic acid, oxalic acid, succinic acid, malonic acid, fatty acid, acetone acid, 1,3-propanedicarboxylic acid, tartaric acid, aspartic acid, salicylic acid, the glycolic.
With deionized water wash microsphere 2-3 time, the collagen in the microsphere is recombinated, and alginate/chitosan/collagen composite microsphere forms.This step is to carry out in room temperature, and washing microsphere is later preserved after lyophilization.Microsphere after the lyophilizing can use with the solvent collocation, and active component is transported to the skin muscular layer safely and effectively.
Embodiment 1:Particle diameter is the alginate/chitosan/collagen composite microsphere of load whitening composition arbutin and the high moisturizing composition ceramide of 1500 μ m
1, as shown in Figure 1, it is in 3.5 the acetic acid aqueous solution that 30mg abortive calfskin type i collagen is dissolved in the 6ml pH value, add the sodium alginate of 2.0ml 6%, 10ml concentration is that arbutin and the 10ml concentration of 6 mg/ml is 1mg/ml ceramide mix homogeneously, and ultrasonic 30s removes bubble.
2, mixed solution is transferred to is connected with gas injection pump (model A-99, Razel scientific instrument company) in the glass syringe, the outside has a pipe that nitrogen cylinder is connected with pump, the air velocity of control pump is the nitrogen of 5 ml/min, solidify 6min in 1 % calcium chloride solution, elementary microsphere forms.In order to prevent collagenous degeneration, this step is carried out at 4 ℃.
3, be that 300,000 chitosan is dissolved in 0.05% acetic acid and obtains the solution that the chitosan mass percent concentration is 1.0 % with molecular weight, its pH is 5.5, elementary microsphere in the step 2 is transferred to further curing in the chitosan solution, and the crosslinking curing time is 5min, and this step is carried out in room temperature.
4, use deionized water wash microsphere 2-3 time, alginate/chitosan/collagen composite microsphere forms.Microsphere is carried out granularmetric analysis, and the microspherulite diameter of preparation is at 1480 ± 50 μ m(Fig. 2,5).Then microsphere is preserved through after the lyophilization.
5, place solvent to carry out swelling the microsphere after the lyophilizing, reach 247% in the 30s swellbility.Wherein solvent is butanediol and hyaluronic aqueous solution, and pH is 6.5, and the concentration of butanediol is 2%.
6, by with microballoon lyophilized, weigh, the weight ratio that obtains sodium alginate, chitosan, Ι Collagen Type VI and active component is about: 120:105:30:70.
Embodiment 2:Particle diameter is the alginate/chitosan/collagen composite microsphere of load whitening composition arbutin and the high moisturizing composition ceramide of 500 μ m
Carry out according to the condition among the embodiment 1, change the air velocity of the control pump in the step 2 into 7.5 ml/min by 5 ml/min.Microsphere is carried out granularmetric analysis, and the microspherulite diameter of preparation is at 505 ± 20 μ m(Fig. 3,5).Place solvent to carry out swelling the microsphere after the lyophilizing, reach 315% in the 30s swellbility.
Embodiment 3:Particle diameter is the alginate/chitosan/collagen composite microsphere of three kinds of active component of load of 1500 μ m
Three kinds of active component are respectively whitening composition arbutin, moisturizing composition ceramide, defying age component list skin cell growth factor EGF
Step 1: it is in 3.5 the acetic acid solution that the purified abortive calfskin type i collagen of 30mg is dissolved in the 6ml pH value, 2.0ml 8% sodium alginate, 10ml concentration is that arbutin, the 10ml concentration of 6mg/ml is that 1mg/ml ceramide, 2ml concentration are the EGF(epithelical cell growth factor of 1 μ g/ml) mix homogeneously, ultrasonic 30s removes bubble.
All the other steps are identical with embodiment 1.By with microballoon lyophilized, weigh, the weight ratio that obtains sodium alginate, chitosan, Ι Collagen Type VI and active component is about: 160:140:30:70.
Microsphere is carried out granularmetric analysis, and the microspherulite diameter of preparation is at 1490 ± 50 μ m(Fig. 5).Place solvent to carry out swelling the microsphere after the lyophilizing, reach 268% in the 30s swellbility.
Embodiment 4:Particle diameter is the alginate/chitosan/collagen composite microsphere of three kinds of active component of load of 50 μ m
Three kinds of active component refer to respectively whitening composition arbutin, moisturizing composition ceramide, defying age component list skin cell growth factor EGF
Carry out according to the condition among the embodiment 3, change the air velocity of the control pump in the step 2 into 12 ml/min by 5 ml/min.Microsphere is carried out granularmetric analysis, and the microspherulite diameter of preparation is at 53 ± 9 μ m(Fig. 5).Place solvent to carry out swelling the microsphere after the lyophilizing, reach 367% in the 30s swellbility.
Embodiment 5:The alginate/chitosan of three kinds of active component arbutin of load, ceramide, epithelical cell growth factor EGF/collagen composite microsphere effect is estimated
Study subject: 20 forearm is inboard, and 2cm * the 2cm zone is tested position without age of skin sensitivity history young woman in 25-30 year, and a side is the trial zone, and a side is the check plot
Using method: complex microsphere and solvent collocation are used, spread upon the inboard same position of forearm, concrete using method is, get 2-3 microsphere in the centre of the palm, drip two solvents on microsphere, wait for about 30s, after the abundant swelling of microsphere, softly break to spheroid with forefinger and middle finger, then evenly be applied in forearm, sooner or later respectively once.Continue to use 30 days.
Testing time: since the 1st day, carry out every three days skin test one time, test process is by dermatologist's complete monitoring.
Test index: comprise that cuticlar water is right, melanin and skin elasticity.
The German CK of the right employing of the cuticlar water CM825 of company type moisture tester contact continuous measurement mode is carried out.Skin color adopts the German CK MEXAMETER MX16 of company melanin analyzer to measure.Melanin value is measured by instrument, and average measurement is averaged for 3 times, and measuring range is 0 ~ 999, and it is higher to measure numerical value, illustrates that melanin content is higher in the skin.Skin elasticity adopts the SME575 elasticity tester to observe, and average measurement is averaged for 3 times, uses R
2Value representation (stretching/resilience ratio).
Concrete measurement result sees Table 1, variation tendency is seen Fig. 6, the result shows, behind the active component microsphere (wait and carry solvent) of continuous three kinds of different efficacies of working load, the aggregative indicator of skin all has significant change, right average 46.8 points that improved of cuticlar water, melanin also contrast group has reduced by 49.3 points, and skin elasticity contrast group has increased by 16.3% simultaneously.The active component microsphere of therefore, load different efficacies can satisfy the needs of multifunction nursing articles for use fully.
Claims (10)
1. the alginate/chitosan of carrying active composition/collagen composite microsphere, it is characterized in that being obtained by sodium alginate, chitosan, Ι Collagen Type VI and active fraction preparation, wherein sodium alginate, chitosan, Ι Collagen Type VI and active component weight ratio are 3 ~ 10:2 ~ 9:1 ~ 3:2 ~ 5 in the complex microsphere, described active component is selected from whitening active component, moisturizing active component ceramide and the activity of fighting against senium composition cell growth factor at least two kinds, and the whitening active component is selected from arbutin, fruit acid, anisic acid, aloin.
2. the alginate/chitosan of described carrying active composition/collagen composite microsphere according to claim 1, the particle diameter that it is characterized in that described complex microsphere is 50-1500 μ m.
3. the alginate/chitosan of the described carrying active composition of claim 1/collagen composite method for preparing microsphere is characterized in that comprising the steps:
(1) the Ι Collagen Type VI is dissolved in the acid medium, adds sodium alginate and active component and mix formation homogeneous colloid solution, and remove bubble;
(2) above-mentioned colloid solution is squeezed to the elementary microsphere of formation in the bivalent cation solution;
(3) the elementary microsphere alginate/chitosan that places the chitosan acid solution further to solidify to obtain the carrying active composition/collagen composite microsphere.
4. the alginate/chitosan of described carrying active composition/collagen composite method for preparing microsphere according to claim 3, it is characterized in that: in the step (1), acid medium is organic or inorganic acid, and its pH is 1-4.
5. the alginate/chitosan of described carrying active composition/collagen composite method for preparing microsphere according to claim 3, it is characterized in that: sodium alginate concentration is 0.1-10 % in the colloid solution for preparing in the step (1), Ι Collagen Type VI concentration is 0.1-20 %, and activity component concentration is 0.5-20 %.
6. the alginate/chitosan of described carrying active composition/collagen composite method for preparing microsphere according to claim 3, it is characterized in that: in the step (2), described bivalent cation is selected from Ba
2+, Ca
2+, Co
2+, Zn
2+, Mn
2+, Ni
2+, bivalent cation solution quality percent concentration is 0.1-10 %.
7. the alginate/chitosan of described carrying active composition/collagen composite method for preparing microsphere according to claim 6, it is characterized in that: in the step (2), colloid solution is transferred in the glass syringe that is connected with the gas injection pump, regulate syringe pump speed mixture is squeezed in the bivalent cation solution, form elementary microsphere by gelatification.
8. the alginate/chitosan of described carrying active composition/collagen composite method for preparing microsphere according to claim 3, it is characterized in that: in the step (3), the chitosan acid solution is chitosan to be dissolved in formic acid, acetic acid, propanoic acid, lactic acid, malic acid, citric acid, ascorbic acid, oxalic acid, succinic acid, malonic acid, fatty acid, acetone acid, 1,3-propanedicarboxylic acid, tartaric acid, aspartic acid, salicylic acid or the glycollic acid solution obtain, and the concentration of chitosan is 0.1-10 %.
9. the alginate/chitosan of the described carrying active composition of the claim 1/application of collagen composite microsphere in cosmetics.
10. according to claim 9 described application, it is characterized in that: during use, the alginate/chitosan of carrying active composition/collagen composite microsphere is used after the swelling in solvent, described solvent is butanediol and hyaluronic aqueous solution, pH is 6.0-6.8, and wherein the concentration of butanediol is 2%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210400611.6A CN102885710B (en) | 2012-10-22 | 2012-10-22 | Sodium alga acid/chitosan/collagen composite micro-ball with active components and preparation method of composite micro-ball |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210400611.6A CN102885710B (en) | 2012-10-22 | 2012-10-22 | Sodium alga acid/chitosan/collagen composite micro-ball with active components and preparation method of composite micro-ball |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102885710A true CN102885710A (en) | 2013-01-23 |
| CN102885710B CN102885710B (en) | 2014-10-22 |
Family
ID=47529531
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210400611.6A Active CN102885710B (en) | 2012-10-22 | 2012-10-22 | Sodium alga acid/chitosan/collagen composite micro-ball with active components and preparation method of composite micro-ball |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102885710B (en) |
Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104548189A (en) * | 2015-01-28 | 2015-04-29 | 浙江三创生物科技有限公司 | Microsphere and application thereof in hemostasis of wound |
| CN105380808A (en) * | 2015-11-25 | 2016-03-09 | 陕西艾美雅生物科技有限公司 | Cosmetic substrate, and preparation method and using method thereof |
| CN105380809A (en) * | 2015-11-25 | 2016-03-09 | 陕西艾美雅生物科技有限公司 | Preparation method of solid cosmetic matrix and solid cosmetic matrix |
| CN106492215A (en) * | 2016-12-09 | 2017-03-15 | 长春生物制品研究所有限责任公司 | A kind of preparation method of load-carrying group hepatitis E protein microsphere suspension formulations |
| CN107213032A (en) * | 2016-03-22 | 2017-09-29 | 台钜企业股份有限公司 | Composite powder, method for producing same, and cosmetic composition containing same |
| CN107519093A (en) * | 2017-09-11 | 2017-12-29 | 广州聚注通用技术研究院有限公司 | A kind of crease-resistant eye mask |
| CN107550752A (en) * | 2017-09-11 | 2018-01-09 | 广州聚注通用技术研究院有限公司 | A kind of crease-resistant eye mask that compacts |
| CN108653041A (en) * | 2018-07-11 | 2018-10-16 | 高昕文 | A kind of preparation method of the newborn moisturizing mask of camel |
| CN109528692A (en) * | 2018-12-03 | 2019-03-29 | 王丽萍 | Growth factor slow-release freeze-drying film and preparation method thereof is concentrated |
| CN110054396A (en) * | 2018-01-18 | 2019-07-26 | 宁波大学 | A kind of 2S2G chalcogenide glass microballoon producing device |
| WO2020192557A1 (en) * | 2019-03-27 | 2020-10-01 | 泓博元生命科技（深圳）有限公司 | Nadh or nadph-containing nano-microsphere and preparation method therefor and application thereof |
| CN111728892A (en) * | 2020-08-14 | 2020-10-02 | 宋家豪 | Oil-control cosmetic cream for improving oily skin and preparation method thereof |
| CN112842931A (en) * | 2021-02-04 | 2021-05-28 | 甘肃天际生物科技有限公司 | Animal collagen freeze-dried ball, cosmetic set and preparation process |
| CN114053965A (en) * | 2021-12-08 | 2022-02-18 | 罗莱生活科技股份有限公司 | Ceramide microcapsule, modified viscose fiber, and preparation method and application thereof |
| CN114177112A (en) * | 2021-12-29 | 2022-03-15 | 润辉生物技术（威海）有限公司 | Application of acetyl sodium alginate in cosmetics, functional cosmetics and preparation method |
| CN114177112B (en) * | 2021-12-29 | 2024-04-26 | 润辉生物技术（威海）有限公司 | Application of acetyl sodium alginate in cosmetics, functional cosmetics and preparation method |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6458387B1 (en) * | 1999-10-18 | 2002-10-01 | Epic Therapeutics, Inc. | Sustained release microspheres |
| CN1380109A (en) * | 2002-04-17 | 2002-11-20 | 苗九昌 | Chitosan collagen and calcium alginate compounded spongy biological dressing and its preparation process |
| CN101461785A (en) * | 2009-01-08 | 2009-06-24 | 上海交通大学 | Oil in water-oil in oil-water in oil method for preparing microballoons |
| CN101485627A (en) * | 2009-01-08 | 2009-07-22 | 上海交通大学 | Microsphere prepared from glycerol modified solid-in-oil-in-water and preparation method thereof |
| KR20110035134A (en) * | 2009-09-29 | 2011-04-06 | 중앙대학교 산학협력단 | Preparation method for superparamagnetic iron oxide nanoparticles (spios)-loaded polymeric microspheres |
-
2012
- 2012-10-22 CN CN201210400611.6A patent/CN102885710B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6458387B1 (en) * | 1999-10-18 | 2002-10-01 | Epic Therapeutics, Inc. | Sustained release microspheres |
| CN1380109A (en) * | 2002-04-17 | 2002-11-20 | 苗九昌 | Chitosan collagen and calcium alginate compounded spongy biological dressing and its preparation process |
| CN101461785A (en) * | 2009-01-08 | 2009-06-24 | 上海交通大学 | Oil in water-oil in oil-water in oil method for preparing microballoons |
| CN101485627A (en) * | 2009-01-08 | 2009-07-22 | 上海交通大学 | Microsphere prepared from glycerol modified solid-in-oil-in-water and preparation method thereof |
| KR20110035134A (en) * | 2009-09-29 | 2011-04-06 | 중앙대학교 산학협력단 | Preparation method for superparamagnetic iron oxide nanoparticles (spios)-loaded polymeric microspheres |
Cited By (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104548189A (en) * | 2015-01-28 | 2015-04-29 | 浙江三创生物科技有限公司 | Microsphere and application thereof in hemostasis of wound |
| CN105380808A (en) * | 2015-11-25 | 2016-03-09 | 陕西艾美雅生物科技有限公司 | Cosmetic substrate, and preparation method and using method thereof |
| CN105380809A (en) * | 2015-11-25 | 2016-03-09 | 陕西艾美雅生物科技有限公司 | Preparation method of solid cosmetic matrix and solid cosmetic matrix |
| CN107213032A (en) * | 2016-03-22 | 2017-09-29 | 台钜企业股份有限公司 | Composite powder, method for producing same, and cosmetic composition containing same |
| CN106492215A (en) * | 2016-12-09 | 2017-03-15 | 长春生物制品研究所有限责任公司 | A kind of preparation method of load-carrying group hepatitis E protein microsphere suspension formulations |
| CN107519093A (en) * | 2017-09-11 | 2017-12-29 | 广州聚注通用技术研究院有限公司 | A kind of crease-resistant eye mask |
| CN107550752A (en) * | 2017-09-11 | 2018-01-09 | 广州聚注通用技术研究院有限公司 | A kind of crease-resistant eye mask that compacts |
| CN110054396A (en) * | 2018-01-18 | 2019-07-26 | 宁波大学 | A kind of 2S2G chalcogenide glass microballoon producing device |
| CN108653041A (en) * | 2018-07-11 | 2018-10-16 | 高昕文 | A kind of preparation method of the newborn moisturizing mask of camel |
| CN109528692A (en) * | 2018-12-03 | 2019-03-29 | 王丽萍 | Growth factor slow-release freeze-drying film and preparation method thereof is concentrated |
| CN109528692B (en) * | 2018-12-03 | 2020-11-10 | 王丽萍 | Concentrated growth factor slow-release freeze-dried film and preparation method thereof |
| WO2020192557A1 (en) * | 2019-03-27 | 2020-10-01 | 泓博元生命科技（深圳）有限公司 | Nadh or nadph-containing nano-microsphere and preparation method therefor and application thereof |
| CN111728892A (en) * | 2020-08-14 | 2020-10-02 | 宋家豪 | Oil-control cosmetic cream for improving oily skin and preparation method thereof |
| CN112842931A (en) * | 2021-02-04 | 2021-05-28 | 甘肃天际生物科技有限公司 | Animal collagen freeze-dried ball, cosmetic set and preparation process |
| CN114053965A (en) * | 2021-12-08 | 2022-02-18 | 罗莱生活科技股份有限公司 | Ceramide microcapsule, modified viscose fiber, and preparation method and application thereof |
| CN114177112A (en) * | 2021-12-29 | 2022-03-15 | 润辉生物技术（威海）有限公司 | Application of acetyl sodium alginate in cosmetics, functional cosmetics and preparation method |
| CN114177112B (en) * | 2021-12-29 | 2024-04-26 | 润辉生物技术（威海）有限公司 | Application of acetyl sodium alginate in cosmetics, functional cosmetics and preparation method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102885710B (en) | 2014-10-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102885710B (en) | Sodium alga acid/chitosan/collagen composite micro-ball with active components and preparation method of composite micro-ball | |
| Chen et al. | Photothermal-promoted multi-functional dual network polysaccharide hydrogel adhesive for infected and susceptible wound healing | |
| Hsieh et al. | Preparation of γ-PGA/chitosan composite tissue engineering matrices | |
| US20100239621A1 (en) | Gel sheet and cosmetic preparation in sheet form using the same | |
| Zhou et al. | Release properties of tannic acid from hydrogen bond driven antioxidative cellulose nanofibrous films | |
| AU2013333184B2 (en) | Hyaluronic acid gel and method for producing same | |
| US20100221306A1 (en) | Adhesive gel sheet for living organisms and sheet form cosmetics comprising the same | |
| Nurani et al. | Preparation and characterization of metformin surface modified cellulose nanofiber gel and evaluation of its anti-metastatic potentials | |
| Dinu et al. | Physically cross-linked chitosan/dextrin cryogels entrapping Thymus vulgaris essential oil with enhanced mechanical, antioxidant and antifungal properties | |
| CN106029047A (en) | Cosmetic gel sheet and production method therefor | |
| AU2017257932B2 (en) | Alcogel sheet for cosmetic use, and method for producing same | |
| CN104739657A (en) | Collagen gel containing nanogold, and prepetition method and application thereof | |
| Feng et al. | Exploring the role of chitosan in affecting the adhesive, rheological and antimicrobial properties of carboxymethyl cellulose composite hydrogels | |
| CN103861536A (en) | Blumea oil composite microcapsule, preparation method thereof and multifunctional textile preparation method | |
| CN114010532A (en) | Double-agent unidirectional slow-release skin repairing film and preparation method and application thereof | |
| KR20180069488A (en) | Dissolvable film comprising spicule and use thereof | |
| Mutreja et al. | Chitin and chitosan: Current status and future opportunities | |
| Al-Musawi et al. | Electrospun Polyvinylpyrrolidone/Soy protein nanofibers enriched with curcumin-loaded ZIF-8 nanoparticles: Synthesis and evaluation for wound healing application | |
| Gutierrez-Reyes et al. | Smart collagen/xanthan gum-based hydrogels with antibacterial effect, drug release capacity and excellent performance in vitro bioactivity for wound healing application | |
| CN104490933B (en) | Crocodile blood nano microcapsule and preparation method thereof | |
| Ma et al. | Temperature and pH-dependent nanogel for smart pesticide delivery with enhanced foliar dispersion and washout resistance can effectively control multiple plant diseases | |
| CN102766267A (en) | Preparation method of chitosan hydrogel containing magnetic nanoparticle | |
| JP5805522B2 (en) | Powder composite and wound dressing | |
| CN105770989B (en) | A kind of biomaterial and its method for personalized preparation breast prosthesis's substitute | |
| CN103570952B (en) | Method for self-assembling heptapeptide derivative Fmoc-7AAP into hydrogel in sodium alginate solution |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |