CN102884945A - White fungus strain breeding method - Google Patents
White fungus strain breeding method Download PDFInfo
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- CN102884945A CN102884945A CN2012104200027A CN201210420002A CN102884945A CN 102884945 A CN102884945 A CN 102884945A CN 2012104200027 A CN2012104200027 A CN 2012104200027A CN 201210420002 A CN201210420002 A CN 201210420002A CN 102884945 A CN102884945 A CN 102884945A
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Abstract
The invention relates to a white fungus strain breeding method which belongs to the technical field of edible fungus cultivation. The method comprises the steps of extracting pure white hyphae of the white fungus, extracting the feathery hyphae and carrying out intercourse cultivation on the two hyphae to form stock culture, wherein the judgment of the mycelium feature is combined in the extraction processes of the two hyphae and a unified standard is divided for selecting the source of excellent mycelium. According to the white fungus strain breeding method, the intercourse proportion of the pure white hyphae to the feathery hyphae is proper, so that the characteristic of rapidness in early development is provided; the feathery hyphae are good in growth vigor at the earlier stage and can grow strongly before the generation of ammoniac odor, so that the high yield, the high immunity and the stability of the strain are ensured; and the merits of the extracted pure white hyphae and the feathery hyphae are determined via combining the judgment of the mycelium feature, so that the high repeatability of the white fungus strain preparation is ensured.
Description
Technical field
The invention belongs to the fungus growing technique field, relate to a kind of white fungus bacterial classification mating system.
Background technology
White fungus, having another name called tremella, tremella, is the mountain delicacy mushroom that China has long enjoyed a good reputation.Be subordinate to and Basidiomycetes Tremellales, Tremellaceae, Tremella.White fungus is the famous edible medicinal fungus of holding concurrently, and nutrition is very abundant.Thereby have higher medical value, also contain multiple essential amino acid.Along with the development growth of mushroom industry, the production of white fungus has now become one of most of peasant's Main Economic source of certain areas.Bacterial classification like the seed of crops, is the basic of Edible Fungi, and its quality directly affects output and the quality of edible mushroom.The bacterial classification of artificial culture have female kind, original seed and cultivated species minute, the female kind is to separate with spore separation or tissue to cultivate the mycelium that forms, mother is planted the bacterial classification that expands on the composts or fertilisers of cultivating that wood chip, cotton seed hull etc. are the master call original seed, cultivate the bacterial classification that becomes the upper use of production by original seed expansion and be called cultivated species.White fungus history of life is complicated, the making and preserve a few days ago still difficult of the pure mycelia of white fungus.On common PDA medium, mycelial growth is slow with the pure mycelium inoculation of white fungus, and easily changes the easy flavescence of mycelia, gelatinize to arthrospore.The pure mycelia of white fungus does not have the ability of decomposing lignocellulose, only has when white fungus mycelia and ear friend mycelia (claiming again the incense ashes bacterium, a kind of fungi of Ascomycotina) is living together could grow and ties ear.For overcoming bacterial classification that traditional tremella fuciformis strain culture method the cultivates defective unstable and that white fungus output is not high of easily degenerating, grow, on October 12nd, 2009, the patent No. CN 102037849A of Jiang Shougen invention discloses a kind of tremella fuciformis strain culture method, by picking out cultivations of joining of the strong incense ashes mycelia of the mycelia that sprouts of the strong good tremella spore of growth and growth, thus cultivate resistance strong, be difficult for degenerating, growth is stablized, output is high white fungus bacterial classification.Hold yet the white fungus mycelia of choosing joining and the mycelia feature of incense ashes mycelia and the ratio of joining lack, cause the white fungus bacterial classification repeatability cultivated not high.
Summary of the invention
In order to overcome above shortcomings part in the prior art, the object of the present invention is to provide a kind of white fungus bacterial classification mating system, the white fungus bacterial classification of cultivate that a kind of repeatability is high, resistance is strong, be difficult for degenerating, growth is stable, output is high.
In order to reach above-mentioned purpose, the present invention adopts following concrete technical scheme: a kind of white fungus bacterial classification mating system comprises the steps:
(1) extraction of the pure white mycelia of white fungus
The first step is choosing of parting material: plant the cultivating white fungus room from bag, choose sporophore shape and be that peony shape, auricle are plump, color and luster bright white, without the desirable ear that damage by disease and insect infects, place microscopically to observe and conscientiously identify after, elect parting material as;
Second step is with sterile water suck dry moisture after the rinsing repeatedly with parting material;
The 3rd step was to cultivate: parting material is seeded on the sawdust medium, has filled in tampon, 25 ℃ of lower cultivations 7～10 days, until occur the white particulate hypha body on the medium, hypha body is ticked, on the access PDA medium, placed 23～25 ℃ interior the cultivation 10 days of insulating box;
The 4th step was that the pure white mycelia feature of good white fungus is judged, its criterion is: the mycelia color is for white, faint yellow or yellow; Aerial hyphae uprightly or tiltedly stands smooth in media surface, and substrate mycelium is born in the medium the inside, and its hyphal diameter is 1.5～3 microns, and diaphragm is arranged, and the part mycelium has obvious clamp connexion;
The 5th step was the extraction of the pure white mycelia of white fungus: by observing, according to the criterion in the 4th step, select the mycelia tendency good, climb wall power strong, unfold mycelia magnificent as the pure white mycelia of white fungus;
(2) extraction of incense ashes mycelia
The first step is choosing of parting material: support basic deep layer at bag cultivating, get a point and contain mycelial wood chip;
Second step is to cultivate: parting material is seeded on the PDA medium, under 23～26 ℃ of conditions, cultivated 3～4 days, the inoculation block edge grows the mycelia of the thick shape of white, the mycelium color transfers yellow green to by white, growth along with mycelia, secrete melanin, impel medium color gradually blackening and constantly intensification;
The 3rd step was that good incense ashes mycelia feature is judged, its criterion is: white mycelium, featheriness, aerial hyphae are canescence, fine fleece shape, and the black scar of carbonaceous is arranged sometimes; Conidiophore is the broom shape, conidium be yellow green to grass green, near oval, 3～5 microns of diameters;
The 4th step was the extraction of incense ashes mycelia: by observing, according to the criterion in the 3rd step, select growing way prosperous, climb the strong featheriness mycelia of wall power, take the tip partition method, mycelium is picked out, and through transplanting for several times, obtains featheriness incense ashes mycelia;
(3) two kinds of mycelia join and cultivate into female the kind: adopt the slant tube cultivating method of joining, the central authorities of the pure white mycelia access of white fungus test-tube culture medium, a little incense ashes mycelia of picking, be connected to from 1～2 centimetre of position of the pure white mycelia of white fungus, after the two is joined, the incense ashes mycelia is black and is covered with in vitro whole medium, and the yellowish red color globule occurs on the inoculation piece of the pure white mycelia of white fungus.
The pure white bacterium of white fungus and the incense ashes bacterium ratio of joining are one thousandth in the described step (3).
Described two kinds of mycelia join and cultivate into female the kind and also can carry out at sawdust medium, first the pure white mycelia of white fungus together with medium a little picking is out from test tube, put on the sawdust medium, then extract the incense ashes mycelia of a little, put into the pure white mycelia of white fungus next door or above, 23～25 ℃ of lower cultivations, on the white hair ball of inoculation piece the yellowish red color globule appears when the two is joined.
Compared with prior art, the present invention has following outstanding advantages and effect: the present invention joins by the pure white mycelia of white fungus and incense ashes mycelia and cultivates the white fungus bacterial classification, because the pure white mycelia of white fungus and incense ashes mycelia ratio are suitable, the fast characteristics of early development are arranged, incense ashes mycelia growing way in early stage is good, can catch up with before ammonia flavor produces with regard to strong growth, guarantee the stability of the high yield of bacterial classification, high resistance and bacterial classification; And the pure white mycelia of white fungus of extraction and the merit of incense ashes mycelia are determined in the judgement of ligative hyphae body characteristics, have guaranteed the high duplication of white fungus bacterial classification preparation.
Embodiment:
Following example further specifies the present invention, but should be as restriction of the present invention.
Beat cave, sterilization, cooling, inoculation and mycelia by composts or fertilisers of cultivating making, pack and cultivate an acquisition bag cultivation white fungus, then finish breeding of white fungus bacterial classification by following three link:
(1) extraction of the pure white mycelia of white fungus
The first step is choosing of parting material: plant the cultivating white fungus room from bag, choose sporophore shape and be that peony shape, auricle are plump, color and luster bright white, without the desirable ear that damage by disease and insect infects, place microscopically to observe and conscientiously identify after, elect parting material as;
Second step is with sterile water suck dry moisture after the rinsing repeatedly with parting material;
The 3rd step was to cultivate: parting material is seeded to the (material that sawdust medium is formed by wood chip 75%, oatmeal 20%, land plaster 5% on the sawdust medium, to press 1:1.2 formulated with water), filled in tampon, 25 ℃ of lower cultivations 7～10 days, until occur the white particulate hypha body on the medium, hypha body is ticked, (the PDA medium is by potato 200g/L on the access PDA medium, glucose 20g/L, agar 18g/L is formulated), place in 23～25 ℃ the insulating box and cultivated 10 days;
The 4th step was that the pure white mycelia feature of good white fungus is judged, its criterion is: the mycelia color is for white, faint yellow or yellow; Aerial hyphae uprightly or tiltedly stands smooth in media surface, and substrate mycelium is born in the medium the inside, and its hyphal diameter is 1.5～3 microns, and diaphragm is arranged, and the part mycelium has obvious clamp connexion;
The 5th step was the extraction of the pure white mycelia of white fungus: by observing, according to the criterion in the 4th step, select the mycelia tendency good, climb wall power strong, unfold mycelia magnificent as the pure white mycelia of white fungus;
(2) extraction of incense ashes mycelia
The first step is choosing of parting material: support basic deep layer at bag cultivating, get a point and contain mycelial wood chip;
Second step is to cultivate: parting material is seeded on the PDA medium, under 23～26 ℃ of conditions, cultivated 3～4 days, the inoculation block edge grows the mycelia of the thick shape of white, the mycelium color transfers yellow green to by white, growth along with mycelia, secrete melanin, impel medium color gradually blackening and constantly intensification;
The 3rd step was that good incense ashes mycelia feature is judged, its criterion is: white mycelium, featheriness, aerial hyphae are canescence, fine fleece shape, and the black scar of carbonaceous is arranged sometimes; Conidiophore is the broom shape, conidium be yellow green to grass green, near oval, 3～5 microns of diameters;
The 4th step was the extraction of incense ashes mycelia: by observing, according to the criterion in the 3rd step, select growing way prosperous, climb the strong featheriness mycelia of wall power, take the tip partition method, mycelium is picked out, and through transplanting for several times, obtains featheriness incense ashes mycelia;
(3) two kinds of mycelia join and cultivate into female the kind: the pure white bacterium of white fungus and the incense ashes bacterium ratio of joining are one thousandth, adopt the slant tube cultivating method of joining, the central authorities of the pure white mycelia access of white fungus test-tube culture medium, a little incense ashes mycelia of picking, be connected to from 1～2 centimetre of position of the pure white mycelia of white fungus, after the two was joined, the incense ashes mycelia was black and is covered with in vitro whole medium, and the yellowish red color globule occurs on the inoculation piece of the pure white mycelia of white fungus.
Claims (3)
1. a white fungus bacterial classification mating system is characterized in that comprising the steps:
The extraction of the pure white mycelia of white fungus
The first step is choosing of parting material: plant the cultivating white fungus room from bag, choose sporophore shape and be that peony shape, auricle are plump, color and luster bright white, without the desirable ear that damage by disease and insect infects, place microscopically to observe and conscientiously identify after, elect parting material as;
Second step is with sterile water suck dry moisture after the rinsing repeatedly with parting material;
The 3rd step was to cultivate: parting material is seeded on the sawdust medium, has filled in tampon, 25 ℃ of lower cultivations 7～10 days, until occur the white particulate hypha body on the medium, hypha body is ticked, on the access PDA medium, placed 23～25 ℃ interior the cultivation 10 days of insulating box;
The 4th step was that the pure white mycelia feature of good white fungus is judged, its criterion is: the mycelia color is for white, faint yellow or yellow; Aerial hyphae uprightly or tiltedly stands smooth in media surface, and substrate mycelium is born in the medium the inside, and its hyphal diameter is 1.5～3 microns, and diaphragm is arranged, and the part mycelium has obvious clamp connexion;
The 5th step was the extraction of the pure white mycelia of white fungus: by observing, according to the criterion in the 4th step, select the mycelia tendency good, climb wall power strong, unfold mycelia magnificent as the pure white mycelia of white fungus;
The extraction of incense ashes mycelia
The first step is choosing of parting material: support basic deep layer at bag cultivating, get a point and contain mycelial wood chip;
Second step is to cultivate: parting material is seeded on the PDA medium, under 23～26 ℃ of conditions, cultivated 3～4 days, the inoculation block edge grows the mycelia of the thick shape of white, the mycelium color transfers yellow green to by white, growth along with mycelia, secrete melanin, impel medium color gradually blackening and constantly intensification;
The 3rd step was that good incense ashes mycelia feature is judged, its criterion is: white mycelium, featheriness, aerial hyphae are canescence, fine fleece shape, and the black scar of carbonaceous is arranged sometimes; Conidiophore is the broom shape, conidium be yellow green to grass green, near oval, 3～5 microns of diameters;
The 4th step was the extraction of incense ashes mycelia: by observing, according to the criterion in the 3rd step, select growing way prosperous, climb the strong featheriness mycelia of wall power, take the tip partition method, mycelium is picked out, and through transplanting for several times, obtains featheriness incense ashes mycelia;
Two kinds of mycelia join and cultivate into female the kind: adopt the slant tube cultivating method of joining, the central authorities of the pure white mycelia access of white fungus test-tube culture medium, a little incense ashes mycelia of picking, be connected to from 1～2 centimetre of position of the pure white mycelia of white fungus, after the two is joined, the incense ashes mycelia is black and is covered with in vitro whole medium, and the yellowish red color globule occurs on the inoculation piece of the pure white mycelia of white fungus.
2. a kind of white fungus bacterial classification mating system according to claim 1 is characterized in that the middle pure white bacterium of white fungus of link (3) and the incense ashes bacterium ratio of joining are one thousandth.
3. a kind of white fungus bacterial classification mating system according to claim 1, it is characterized in that two kinds of mycelia join cultivates into female the kind and also can carry out at sawdust medium, first the pure white mycelia of white fungus together with medium a little picking is out from test tube, put on the sawdust medium, then extract the incense ashes mycelia of a little, put into the pure white mycelia of white fungus next door or above, 23～25 ℃ of lower cultivations, on the white hair ball of inoculation piece the yellowish red color globule appears when the two is joined.
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103493680A (en) * | 2013-09-23 | 2014-01-08 | 重庆市中药研究院 | Tremella liquid cultivar culture method and special culture medium for cultivar |
| CN103503693A (en) * | 2013-09-30 | 2014-01-15 | 东北林业大学 | Method for rapidly separating auricularia auricula strains by utilization of dry auricles |
| CN104798598A (en) * | 2015-04-09 | 2015-07-29 | 雷德赐 | Production technique of liquid tremella fuciformis strains applicable to bag-planting, tin-planting and mechanical inoculation |
| CN105112297A (en) * | 2015-08-05 | 2015-12-02 | 福建农林大学 | Culture medium for cultivating Hypoxylon sp. not producing melanin |
| CN106613358A (en) * | 2016-12-30 | 2017-05-10 | 湖南味菇坊生物科技有限公司 | White fungus spawn extracting method |
| CN107347461A (en) * | 2017-09-15 | 2017-11-17 | 福建古银生物技术有限公司 | A kind of method of comprehensive utilization of bamboo resource |
| CN111034537A (en) * | 2019-12-23 | 2020-04-21 | 广州市金因源生物技术有限公司 | Preparation method for quickly separating pure tremella hyphae and mother tremella seed |
| CN112021067A (en) * | 2020-09-10 | 2020-12-04 | 云南菌视界生物科技有限公司 | Method for breeding new tremella aurantialba variety through combination of asexual spores of tremella aurantialba and stereum hirsutum |
| CN112042465A (en) * | 2020-09-10 | 2020-12-08 | 云南菌视界生物科技有限公司 | Method for breeding new tremella aurantialba variety through combination of tremella aurantialba sporocarp and exogenous stereum hirsutum |
| WO2021109291A1 (en) * | 2019-12-05 | 2021-06-10 | 山东安华生物医药股份有限公司 | Method for cultivating tremella strain |
| CN116948846A (en) * | 2023-09-19 | 2023-10-27 | 江西农业大学 | Method for separating and purifying colloid bacteria |
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Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103493680A (en) * | 2013-09-23 | 2014-01-08 | 重庆市中药研究院 | Tremella liquid cultivar culture method and special culture medium for cultivar |
| CN103503693A (en) * | 2013-09-30 | 2014-01-15 | 东北林业大学 | Method for rapidly separating auricularia auricula strains by utilization of dry auricles |
| CN103503693B (en) * | 2013-09-30 | 2015-09-23 | 东北林业大学 | A kind of method utilizing dry auricle quick separating edible fungus |
| CN104798598A (en) * | 2015-04-09 | 2015-07-29 | 雷德赐 | Production technique of liquid tremella fuciformis strains applicable to bag-planting, tin-planting and mechanical inoculation |
| CN105112297A (en) * | 2015-08-05 | 2015-12-02 | 福建农林大学 | Culture medium for cultivating Hypoxylon sp. not producing melanin |
| CN105112297B (en) * | 2015-08-05 | 2018-08-17 | 福建农林大学 | A kind of incense ashes bacterium does not produce the culture medium of melanin |
| CN106613358A (en) * | 2016-12-30 | 2017-05-10 | 湖南味菇坊生物科技有限公司 | White fungus spawn extracting method |
| CN107347461A (en) * | 2017-09-15 | 2017-11-17 | 福建古银生物技术有限公司 | A kind of method of comprehensive utilization of bamboo resource |
| WO2021109291A1 (en) * | 2019-12-05 | 2021-06-10 | 山东安华生物医药股份有限公司 | Method for cultivating tremella strain |
| CN111034537A (en) * | 2019-12-23 | 2020-04-21 | 广州市金因源生物技术有限公司 | Preparation method for quickly separating pure tremella hyphae and mother tremella seed |
| CN111034537B (en) * | 2019-12-23 | 2021-09-28 | 广州市金因源生物技术有限公司 | Preparation method for quickly separating pure tremella hyphae and mother tremella seed |
| CN112042465A (en) * | 2020-09-10 | 2020-12-08 | 云南菌视界生物科技有限公司 | Method for breeding new tremella aurantialba variety through combination of tremella aurantialba sporocarp and exogenous stereum hirsutum |
| CN112021067A (en) * | 2020-09-10 | 2020-12-04 | 云南菌视界生物科技有限公司 | Method for breeding new tremella aurantialba variety through combination of asexual spores of tremella aurantialba and stereum hirsutum |
| CN112042465B (en) * | 2020-09-10 | 2022-08-09 | 云南菌视界生物科技有限公司 | Method for breeding new tremella aurantialba variety through combination of tremella aurantialba sporocarp and exogenous stereum hirsutum |
| CN116948846A (en) * | 2023-09-19 | 2023-10-27 | 江西农业大学 | Method for separating and purifying colloid bacteria |
| CN116948846B (en) * | 2023-09-19 | 2023-12-26 | 江西农业大学 | Method for separating and purifying colloid bacteria |
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