CN102866194B - Multivariate electrophoresis interpretoscope - Google Patents
Multivariate electrophoresis interpretoscope Download PDFInfo
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- CN102866194B CN102866194B CN201210325784.6A CN201210325784A CN102866194B CN 102866194 B CN102866194 B CN 102866194B CN 201210325784 A CN201210325784 A CN 201210325784A CN 102866194 B CN102866194 B CN 102866194B
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- electrophoresis
- darkroom
- interpretoscope
- polynary
- tank
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Abstract
The invention relates to techniques of biological chemistry and molecular biology and discloses a multivariate electrophoresis interpretoscope. In the multivariate electrophoresis interpretoscope, a plurality of electrophoresis tanks are densely arranged and form an electrophoresis system with a power supply controller, and a darkroom slides on each electrophoresis tank by pulleys and is combined with each electrophoresis tank to form independent and opposite dark fields. The observing, the photographing and the interpretation can be respectively carried out on gel by sliding the darkroom but not moving the gel, so that an experimenter is protected from directly contacting the gel, the pollutions to instruments, devices and even laboratory environments due to the moving or the transferring of the gel are avoided, the potential experimental risk is avoided, the integration level can be improved, and the use efficiency can be improved. An image processing system is directly integrated into the darkroom, and a display for displaying the image processing result is mounted on the outer surface of the darkroom, so that the reading of the experimental data is facilitated, the integration level of the system is further improved, and the integration is realized.
Description
Technical field
The present invention relates to biological chemistry and Protocols in Molecular Biology, particularly electrophoresis interpretation technology.
Background technology
Charged particle, under electric field action, towards moving with its electrically contrary electrode, is called electrophoresis (electrophoresis is called for short " EP "), utilizes charged particle translational speed in electric field different and reach separated technology and be called electrophoretic techniques.Different charged particles are because of electrically charged difference, although or the electrically charged identical specific charge of institute different, electrophoresis in same electric field, after certain hour, because displacement difference is separated from each other.
By gel electrophoresis, analyze or DNA isolation sample is experimental technique conventional in Biochemical Lab, so DNA electrophoresis apparatus is the essential instrument of each Biochemical Lab.While using existing instrument, experimenter need to transfer to from electrophoresis tank the gel that is loaded with DNA sample darkroom, then observes or take the DNA image in gel with a kind of ultraviolet imaging device.
Therefore, in the prior art, whole experimentation is very consuming time, and in experimentation, electrophoresis process and imaging process are separately carried out, can not continuous imaging in electrophoresis process, and can not carry out in real time the bands of a spectrum in electrophoresis process, dynamically observe.And the most frequently used method of DNA in gel of observing is to utilize fluorescent dye ethidium bromide (EB) to dye, EB molecule can embed between the base-pair of nucleic acid double chain, under ultraviolet ray excited, sends red fluorescence.Its fluorescence intensity is directly proportional to the content of DNA, accordingly can guestimate sample DNA concentration, and Ago-Gel EB dyeing, the visible nucleic acid electrophoresis band of naked eyes, its DNA content is generally greater than 5ng.EB is strong mutagen, has high carcinogenic, in experimentation, between electrophoresis tank and darkroom, experimenter with hand transmission, pick and place gel, even with protective gloves, also very easily to the person, instrument and environment.In addition, because EB easily volatilizees, very easily cause air pollution, EB can absorb by skin contact or through respiratory tract.Erect image we utilize EB the same with the characteristic of nucleic acid combination, once EB is absorbed by the body, it is very easily combined with the gene of human body, thereby have the possibility of the sudden change or other harm that cause human body gene.Therefore, the danger of EB can not be ignored.
In addition, electrophoresis tank, darkroom and imaging device are discrete to be put, and the underintegration of system need to occupy larger research platform, and when user is more, service efficiency is low.
Summary of the invention
The object of the present invention is to provide a kind of polynary electrophoresis interpretoscope, by slip darkroom, just can be to the interpretation of taking pictures respectively of the gel in each electrophoresis tank without removable gel, avoided experimenter and the direct of gel to contact, avoided potential experiment risk, and can improve integrated level, improve the service efficiency of instrument.
For solving the problems of the technologies described above, embodiments of the present invention disclose a kind of polynary electrophoresis interpretoscope, comprising:
At least two electrophoresis tanks, two rail plates with Guide rollers, darkroom, image processing system and electrophoresis powers;
On each electrophoresis tank, electrode is installed, electrophoresis power is powered for each electrophoresis tank by this electrode;
Each electrophoresis tank is arranged side by side, and rail plate is fixed on the both sides of each electrophoresis tank;
Thereby darkroom strides across electrophoresis tank to be connected and can be slided along rail plate in darkroom with Guide rollers on rail plate, there is an opening below, darkroom in the face of the direction of electrophoresis tank notch;
Camera and ultraviolet light source are installed in darkroom, and camera is connected with image processing system;
Make by lighttight material in each electrophoresis tank and darkroom.
Compared with prior art, the key distinction and effect thereof are embodiment of the present invention:
A plurality of electrophoresis tanks of dense arrangement, electrophoresis system is placed in electrophoresis tank, slide above electrophoresis tank by pulley in darkroom, combine and form relative dark field with electrophoresis tank, by slip darkroom, without removable gel, just can the gel in each electrophoresis tank be observed respectively, be taken pictures and interpretation, avoided experimenter and the direct of gel to contact, and shift owing to moving the pollution to instrument, equipment and even laboratory environment that gel causes, avoided potential experiment risk, and can improve integrated level, improve the service efficiency of instrument.
Further, electrophoresis tank is independent separately, can carry out respectively different electrophoresis experiments, effective conserve space, the service efficiency of raising instrument.
Further, imaging darkroom can be according to experiment needs, and overall process is fixed on a certain electrophoresis tank, so that overall process is observed the dynamic change of electrophoresis, to observe and understand experimental result in time, and need not wait until the last period always.
Further, the existence of electrophoresis power controller, has solved the mixed and disorderly problem that connects up between each electrophoresis tank, has simplified system formation, has improved level of integrated system.
Further, image processing system is directly integrated in darkroom, shows that the display of processing result image is arranged on the outside surface in darkroom, is convenient to read experimental data, and has further improved the integrated level of system, has realized integrated.
Further, two ultraviolet light sources, can strengthen the uitraviolet intensity in darkroom, better take the result of electrophoresis experiment.
Further, on every rail plate, there are two Guide rollerses, can guarantee stability when slide and take pictures in darkroom on rail plate, thereby can further improve the quality of photographic images.
Accompanying drawing explanation
Fig. 1 is the structural representation of a kind of polynary electrophoresis interpretoscope in first embodiment of the invention;
Fig. 2 is the structural representation of a kind of polynary electrophoresis interpretoscope in second embodiment of the invention;
Fig. 3 is the structural representation of a kind of polynary electrophoresis interpretoscope in third embodiment of the invention.
Embodiment
In the following description, in order to make reader understand the application better, many ins and outs have been proposed.But, persons of ordinary skill in the art may appreciate that even without these ins and outs and the many variations based on following embodiment and modification, also can realize each claim of the application technical scheme required for protection.
For making the object, technical solutions and advantages of the present invention clearer, below in conjunction with accompanying drawing, embodiments of the present invention are described in further detail.
First embodiment of the invention relates to a kind of polynary electrophoresis interpretoscope.Fig. 1 is the structural representation (vertical view) of this polynary electrophoresis interpretoscope.
Specifically, as shown in Figure 1, this polynary electrophoresis interpretoscope comprises:
At least two electrophoresis tanks 1, two rail plates 2 with Guide rollers 3, darkroom 4, image processing system 5 and electrophoresis powers 9.
On each electrophoresis tank 1, electrode 8 is installed, electrophoresis power 9 is each electrophoresis tank 1 power supply by this electrode 8.
Each electrophoresis tank 1 is arranged side by side, and rail plate 2 is fixed on the both sides of each electrophoresis tank 1.
Electrophoresis tank is independent separately, can carry out respectively different electrophoresis experiments, effective conserve space, the service efficiency of raising instrument.
Thereby darkroom 4 strides across electrophoresis tank 1 and is connected with Guide rollers 3 on rail plate 2 and can be slided along rail plate 2 in darkroom 4, there is an opening 4 belows, darkroom in the face of the direction of electrophoresis tank notch.
In the present embodiment, preferably, the notch of darkroom opening and each electrophoresis tank is size on an equal basis.
In the time of directly over darkroom slides into certain electrophoresis tank, darkroom and this electrophoresis tank are combined, and form relative dark field.If the notch of darkroom opening and each electrophoresis tank is size on an equal basis, this relative dark field effect will be more desirable.
Certainly, this is preferred embodiment a kind of, and in some other embodiment of the present invention, the notch of darkroom opening and each electrophoresis tank also can not equal size.
Ultraviolet light source 7 is two quartz burners.
Two ultraviolet light sources, can strengthen the uitraviolet intensity in darkroom, better take the result of electrophoresis experiment.
In addition, be appreciated that, in some other embodiment of the present invention, ultraviolet light source can be also the ultraviolet light source of other type, quantity also can not be two, but one, three or four etc.
Image processing system can receive the signal from camera, it is processed, and have a display can show treated image.The processing mode of image is had multiple, but all belong to prior art, in the application, be no longer elaborated.
Make by lighttight material in each electrophoresis tank 1 and darkroom 4.
On every rail plate 2, there are two Guide rollerses 3.
On every rail plate, have two Guide rollerses, by this Guide rollers, can slide in darkroom on rail plate.
In addition, screens or the lock-bit that on guide rail, in the corresponding position of each electrophoresis tank, can arrange, for the fixing position in darkroom, so that the stability while guaranteeing that darkroom is observed and takes pictures on rail plate, thereby can further improve the quality of photographic images.
In experimentation, darkroom can be according to experiment needs, and overall process is fixed on a certain electrophoresis tank top, so that overall process is observed the dynamic change of electrophoresis in this electrophoresis tank, observes and understands experimental result in time, and need not wait until the last period always.
In addition, be appreciated that Guide rollers also can not be two, but more in some other embodiment of the present invention, on guide rail, also screens or lock-bit can be set.
The middle part of each electrophoresis tank 1 is provided with gel platform, and this gel platform is made by white or transparent material, for placing gel 10.
The axle at camera 6 places is vertical with gel platform.
This polynary electrophoresis interpretoscope also comprises: bracing frame 11, each electrophoresis tank 1 is fixed on this bracing frame 11.
A plurality of electrophoresis tanks of dense arrangement, electrophoresis tank and power-supply controller of electric form electrophoresis system, slide above electrophoresis tank by pulley in darkroom, combine and form relative dark field with electrophoresis tank, by slip darkroom, just can be to the interpretation of taking pictures respectively of the gel in each electrophoresis tank without removable gel, avoided experimenter and the direct of gel to contact, and shift owing to moving the pollution to instrument, equipment and even laboratory environment that gel causes, avoided potential experiment risk, and can improve integrated level, improve the service efficiency of instrument.
Second embodiment of the invention relates to a kind of polynary electrophoresis interpretoscope.Fig. 2 is the structural representation (side view) of this polynary electrophoresis interpretoscope.
The second embodiment improves on the basis of the first embodiment, and main improvements are:
Specifically, as shown in Figure 2, this polynary electrophoresis interpretoscope also comprises:
The middle part of each electrophoresis tank 1 is provided with gel platform 12, for placing gel 10.
The existence of electrophoresis power controller, has solved the mixed and disorderly problem that connects up between each electrophoresis tank, has simplified system formation, has improved level of integrated system.
Third embodiment of the invention relates to a kind of polynary electrophoresis interpretoscope.Fig. 3 is the structural representation (side view) of this polynary electrophoresis interpretoscope.
The 3rd embodiment improves on the basis of the second embodiment, and main improvements are:
Image processing system is directly integrated in darkroom, shows that the display of processing result image is arranged on the outside surface in darkroom, is convenient to read experimental data, and has further improved the integrated level of system, has realized integrated.
It should be noted that, in the claim and instructions of this patent, relational terms such as the first and second grades is only used for an entity or operation to separate with another entity or operational zone, and not necessarily requires or imply and between these entities or operation, have the relation of any this reality or sequentially.And, term " comprises ", " comprising " or its any other variant are intended to contain comprising of nonexcludability, thereby the process, method, article or the equipment that make to comprise a series of key elements not only comprise those key elements, but also comprise other key elements of clearly not listing, or be also included as the intrinsic key element of this process, method, article or equipment.The in the situation that of more restrictions not, the key element that " comprises " and limit by statement, and be not precluded within process, method, article or the equipment that comprises described key element and also have other identical element.
Although pass through with reference to some of the preferred embodiment of the invention, the present invention is illustrated and described, but those of ordinary skill in the art should be understood that and can do various changes to it in the form and details, and without departing from the spirit and scope of the present invention.
Claims (10)
1. a polynary electrophoresis interpretoscope, is characterized in that, comprising:
At least two electrophoresis tanks, two rail plates with Guide rollers, darkroom, image processing system and electrophoresis powers;
On each electrophoresis tank, electrode is installed, electrophoresis power is powered for each electrophoresis tank by this electrode;
Each electrophoresis tank is arranged side by side, and rail plate is fixed on the both sides of each electrophoresis tank;
Thereby darkroom strides across electrophoresis tank to be connected and can be slided along rail plate in darkroom with Guide rollers on rail plate, there is an opening below, darkroom in the face of the direction of electrophoresis tank notch;
Camera and ultraviolet light source are installed in darkroom, and camera is connected with image processing system;
Make by lighttight material in each electrophoresis tank and darkroom.
2. polynary electrophoresis interpretoscope according to claim 1, is characterized in that, described electrophoresis tank is independent separately, is connected respectively with electrophoresis power.
3. polynary electrophoresis interpretoscope according to claim 2, is characterized in that, also comprises:
Electrophoresis power controller, is provided with the power switch corresponding to each electrophoresis tank on this electrophoresis power controller, by controlling this power switch, each electrophoresis tank is carried out to power on/off.
4. according to the polynary electrophoresis interpretoscope described in any one in claims 1 to 3, it is characterized in that, described image processing system is integrated in darkroom, shows that the display of processing result image is arranged on the outside surface in darkroom.
5. polynary electrophoresis interpretoscope according to claim 4, is characterized in that, the notch of darkroom opening and each electrophoresis tank is size on an equal basis.
6. polynary electrophoresis interpretoscope according to claim 5, is characterized in that, described ultraviolet light source is two quartz burners.
7. polynary electrophoresis interpretoscope according to claim 6, is characterized in that, has two Guide rollerses on every rail plate.
8. polynary electrophoresis interpretoscope according to claim 7, is characterized in that, also comprises: bracing frame, described each electrophoresis tank is fixed on this bracing frame.
9. polynary electrophoresis interpretoscope according to claim 8, is characterized in that, the middle part of described each electrophoresis tank is provided with gel platform, and this gel platform is made by white or transparent material, for placing gel.
10. polynary electrophoresis interpretoscope according to claim 9, is characterized in that, the axle at described camera place is vertical with described gel platform.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210325784.6A CN102866194B (en) | 2012-09-05 | 2012-09-05 | Multivariate electrophoresis interpretoscope |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210325784.6A CN102866194B (en) | 2012-09-05 | 2012-09-05 | Multivariate electrophoresis interpretoscope |
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| Publication Number | Publication Date |
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| CN102866194A CN102866194A (en) | 2013-01-09 |
| CN102866194B true CN102866194B (en) | 2014-04-02 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201210325784.6A Expired - Fee Related CN102866194B (en) | 2012-09-05 | 2012-09-05 | Multivariate electrophoresis interpretoscope |
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Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107356653B (en) * | 2017-08-21 | 2023-06-30 | 莫纳（苏州）生物科技有限公司 | Integrated electrophoresis apparatus |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH02143145A (en) * | 1988-11-25 | 1990-06-01 | Hitachi Electron Eng Co Ltd | Electrophoresis device |
| ES2153768B1 (en) * | 1999-03-15 | 2001-11-01 | Tudela Bernardo Hernandez | PERFECTED SEQUENCER OF PROTEINS AND NUCLEIC ACIDS, BY ELECTROPHORESIS, AND PROCEDURE FOR OPERATION. |
| CN2646709Y (en) * | 2003-08-26 | 2004-10-06 | 厦门百维信生物科技有限公司 | Equipment for observing gel electrophoresis in real time |
| JP4712364B2 (en) * | 2004-12-07 | 2011-06-29 | 株式会社アドバンス | Electrophoresis device electrophoresis tank |
| CN1854716B (en) * | 2005-04-18 | 2012-03-21 | 微奥基因科技常州有限公司 | Integrated and light-source variable electrophoretic separating analyzer and its usage |
| CN101865876A (en) * | 2009-04-15 | 2010-10-20 | 上海培清科技有限公司 | Fully automatic molecular electrophoresis gel imaging analyser |
| CN201477073U (en) * | 2009-05-07 | 2010-05-19 | 程浩然 | Agarose gel DNA/RNA electrophoresis apparatus |
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