CN102864199A - Method for producing anti-oxidative polypeptide by using rice residue - Google Patents
Method for producing anti-oxidative polypeptide by using rice residue Download PDFInfo
- Publication number
- CN102864199A CN102864199A CN2012103364848A CN201210336484A CN102864199A CN 102864199 A CN102864199 A CN 102864199A CN 2012103364848 A CN2012103364848 A CN 2012103364848A CN 201210336484 A CN201210336484 A CN 201210336484A CN 102864199 A CN102864199 A CN 102864199A
- Authority
- CN
- China
- Prior art keywords
- subsequent use
- fermentation
- condition
- polypeptide
- dialysis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The invention discloses a method for producing anti-oxidative polypeptide by using rice residue, relating to the technical field of food processing. According to the method, byproduct rice residue obtained by organic acid and antibiotic fermentation and starch sugar production is used as a raw material; the method mainly comprises the following process steps of: A, preparing a mold starter; B, performing post-fermentation; C, performing post-fermentation; D, dialyzing; E, freeze-drying out-of-bag dialysate; F, performing chromatography; and G, freeze-drying chromatographic solution. The method has the characteristics of simple process, less investment, no potential safety hazard of products, capability of producing by using non-water soluble rice residue protein and the like, is suitable for the situations that the rice residue is used as the raw material and other non-water soluble protein is used as the raw material and is used for anti-oxidative polypeptide production.
Description
Technical field
The present invention relates to food processing technology field, particularly a kind of method of utilizing meter slag to produce antioxidation polypeptide.
Background technology
Polypeptide is that a-amino acid links together with peptide chain and the compound that forms, and it also is the intermediate product of proteolysis, is usually formed by 10-100 amino acid molecular dehydrating condensation.Polypeptide compound is easily digested, and have diseases prevention, cure the disease, the effect of balance the body physiological function, particularly it has stronger oxidation-resistance, thereby, being called as antioxidation polypeptide, is a kind of functional food ingredient that has potentiality, becomes gradually the heath food of 21 century.At present, the production method of polypeptide mainly contains acid hydrolyzation, based on alkaline hydrolysis and enzyme process, and mostly adopts soybean or its byproduct as raw materials for production.Adopt dregs of beans as raw material such as Japan, " soybean polypeptide " with acid hydrolyzation is produced as " foodstuff additive ", mainly for the production of old man and infant foods, its objective is to strengthen these two kinds of crowds to the absorption of food nutrition; Since when producing, used acidic chemical material very obstinate, and bitter taste is arranged, need with charcoal absorption, debitterize, and gac unavoidably can be invaded the peptide body.The Production by Enzymes polypeptide is the main stream approach of domestic production polypeptide; Production by Enzymes is with the needed food grade plant protease of human body, and the usual food proteins enzymolysis of eating of human body is become micromolecule active polypeptide; Such as " soybean polypeptide " that Wuhan Jiu Shengtang produces, raw materials used be content at the soybean protein isolate more than 90%, method for hydrolysis is enzyme process, used enzyme belongs to the food grade plant protease; " soybean polypeptide " produced is without bitter taste, and local flavor fragrance is pleasant, and be without any side effects to human body.
The rice slag is take early rice, crack rice, the low value rice such as ageing rice is as raw material, produces the gained by product through organic acid, antibiotic fermentation and β-amylose, and cheap, output is huge, and β-amylose is produced the rice slag that 7 tons of rice of every consumption will produce 1 ton.In starch sugar production process, rice is through high-temperature starch enzyme liquefaction hydrolysis, and last residue behind the Plate Filtration, its protein content be more than 40%, and contains a large amount of dextrin and saccharan.Simultaneously, in the process of rice production β-amylose, protein sex change after pyroprocessing is serious, and the protein characteristic such as water-soluble, whipability, emulsifying property is lost substantially, has limited its Applications in food.All the time, the rice slag only is used as animal-feed, and this is the significant wastage to protein resource.Chinese patent (number of patent application is 200810163917.8) " a kind of rice residue protein that utilizes is produced rice protein polypeptide powder method ", be byproduct rice residue protein take starch sugar refinery as raw material, make the rice protein polypeptide powder of food grade through series of processes such as washing, hydrolysis, desalination, dryings; Protein content 〉=77% in the prepared rice protein polypeptide powder, wherein the little peptide of molecular weight about 1000 accounts for 67% of Tot Prot; The rice protein polypeptide powder has not only kept the hypoallergenic of rice gluten own, advantages such as high biological value, and owing to wherein having contained a large amount of little peptides, digest and assimilate and nourishing function aspect significant function is arranged.
Summary of the invention
The object of the present invention is to provide that a kind of technique is simple, less investment, product are without potential potential safety hazard and can utilize the method for water-insoluble rice residue protein production food grade antioxidation polypeptide.
For achieving the above object, solution of the present invention is a kind of method of utilizing meter slag to produce antioxidation polypeptide of invention, and its technological process is as follows:
A, the bent preparation of kind: with being modulated into the kind koji fermentation material that water content is 45-65% after the boiling of rice slag, add food grade fermented bacterium aspergillus oryzae, its spore inoculating amount is 2-8 * 10 again
6Individual/gram, mix and all be placed under humidity 80-95%, the temperature 25-32 ℃ condition, fermentation culture 3-5 days, become kind of a bent material, for subsequent use;
B, primary fermentation: other is modulated into the fermentation expectation that water content is 45-65% after getting meter slag boiling, adds the kind song that accounting for fermentation expectation weight 1-10% again and expects, mixes thoroughly, place under humidity 80-95%, the temperature 25-32 ℃ condition, fermentation culture 3-5 days, become the primary fermentation material, for subsequent use;
C, secondary fermentation: primary fermentation material for subsequent use is dropped in the sterilized water, and the consumption of its water adds water 60-100ml according to every 10g primary fermentation material and calculates; Then, under 40-42 ℃ of condition, carried out secondary fermentation 5-7 days, become the secondary fermentation material, for subsequent use;
D, dialysis: place 20KD to hold back dialysis tubing in secondary fermentation material for subsequent use, dialysis is 12-24 hour under 3-10 ℃ of condition, and the outer dialyzate of bag taking is for subsequent use;
Dialysis liquid cooling freeze-drying is dry outside E, the bag: the outer dialyzate of bag for subsequent use is carried out lyophilize, and condition is :-30--45 ℃, vacuum tightness 0.4-200Pa gets lyophilized powder, and is for subsequent use;
F, chromatography: lyophilized powder for subsequent use is made into the solution of 10-30mg/ml, crosses Sephadex G-25 dextrane gel, get the chromatographic solution that automatic Fraction Collector is collected, for subsequent use;
G, chromatographic solution lyophilize: chromatographic solution for subsequent use is carried out lyophilize, and condition is :-30--45 ℃, and vacuum tightness 0.4-200Pa, the gained powder is antioxidation polypeptide.
The method of utilizing the rice slag to produce antioxidation polypeptide of the present invention, and adopt kind of bent preparation, primary fermentation and a secondary fermentation three-procedure, rice residue protein can be hydrolyzed into antioxidation polypeptide, thereby its technique is fairly simple, and working condition is easily controlled, need not any specific installation, therefore drop into also fewer; Simultaneously, selected fermented bacterium--aspergillus oryzae is the used bacterial strain of traditional fermentation food, and during the fermentation, neither add soda acid, do not add other any nutrient yet, only utilize the dextrin and the saccharan that are rich in the rice slag, for the microbial growth metabolism provides Carbon and nitrogen sources, be biological hydrolysis, the environmental protection low-carbon (LC); Also do not need follow-up depickling dealkalize, thereby its product is without potential potential safety hazard.
According to detection, the antioxidation polypeptide of producing according to the inventive method, wherein: protein content 〉=88%, molecular weight are between 200Da-2000Da, and it is 79.1%-83.2% that the ratio that molecular weight is lower than 1000Da reaches 70%, DPPH free radical scavenging power.
Embodiment
Below in conjunction with embodiment, the present invention is further illustrated.Following explanation is to adopt the mode that exemplifies, but protection scope of the present invention should not be limited to this.
The method of utilizing the rice slag to produce antioxidation polypeptide of the present invention mainly contains following processing step:
A, the bent preparation of kind: make kind of a bent material;
B, primary fermentation: make the primary fermentation material;
C, secondary fermentation: make the secondary fermentation material;
D, dialysis: make the outer dialyzate of bag;
Dialysis liquid cooling freeze-drying is dry outside E, the bag: make lyophilized powder;
F, chromatography: make chromatographic solution;
G, chromatographic solution lyophilize: make antioxidation polypeptide.
Embodiment one:
A, the bent preparation of kind: get a meter slag and (produce the gained by product through organic acid, antibiotic fermentation and β-amylose, lower same), be modulated into water content after the boiling and be 55% kind koji fermentation material 0.25kg, add food grade fermented bacterium aspergillus oryzae again, the spore inoculating amount is by 4 * 10
6Individual/gram calculates, and is 1 * 10
9Individual spore is mixed and all is placed under humidity 85%, 28 ℃ of conditions of temperature, and fermentation culture 4 days gets the bent material of 0.25kg kind, and is for subsequent use;
B, primary fermentation: other gets and is modulated into water content after meter slag boiling is that 5kg is expected in 55% fermentation, adds the bent material of kind of 0.25kg again, mixes thoroughly, places under humidity 85%, 28 ℃ of conditions of temperature, and fermentation culture 4 days gets 5.25kg primary fermentation material, and is for subsequent use;
C, secondary fermentation: 5.25kg primary fermentation material for subsequent use is dropped in the 42L water, mix all; Then, under 41 ℃ of conditions, carry out secondary fermentation 6 days, get 47.25kg secondary fermentation material, for subsequent use;
D, dialysis: place 20KD to hold back dialysis tubing in 47.25kg secondary fermentation material for subsequent use, dialysis is 18 hours under 7 ℃ of conditions, gets the outer dialyzate of 150L bag, and is for subsequent use;
Dialysis liquid cooling freeze-drying is dry outside E, the bag: at-35 ℃, under the vacuum tightness 100Pa condition, lyophilize gets lyophilized powder 2.82kg, and is for subsequent use with the outer dialyzate of bag for subsequent use.
F, chromatography: lyophilized powder for subsequent use is dissolved into the solution of 20mg/ml, crosses Sephadex G-25 dextrane gel, get the chromatographic solution 30L that automatic Fraction Collector is collected, for subsequent use; In this process, can remove contained salinity (lower same);
G, chromatographic solution lyophilize: chromatographic solution for subsequent use is carried out lyophilize under-35 ℃, vacuum tightness 100Pa condition, get 2.6kg lyophilize powder, be antioxidation polypeptide.
Embodiment two:
A, the bent preparation of kind: get a meter slag and (produce the gained by product through organic acid, antibiotic fermentation and β-amylose, lower same), be modulated into water content after the boiling and be 45% kind koji fermentation material 0.25kg, add food grade fermented bacterium aspergillus oryzae again, the spore inoculating amount is by 2 * 10
6Individual/gram calculates, and is 5 * 10
8Individual spore is mixed all, places under humidity 85%, 28 ℃ of conditions of temperature, and fermentation culture 4 days gets the bent material of 0.25kg kind, and is for subsequent use;
B, primary fermentation: other gets and is modulated into water content after meter slag boiling is that 5kg is expected in 45% fermentation, adds the bent material of kind of 0.25kg again, mixes thoroughly, places under humidity 85%, 28 ℃ of conditions of temperature, and fermentation culture 4 days gets 5.25kg primary fermentation material, and is for subsequent use;
C, secondary fermentation: 5.25kg primary fermentation material for subsequent use is dropped in the 31.5L water, mix all; Then, under 41 ℃ of conditions, carry out secondary fermentation 6 days, get 36.75kg secondary fermentation material, for subsequent use;
D, dialysis: place 20KD to hold back dialysis tubing in 36.75kg secondary fermentation material for subsequent use, dialysis is 18 hours under 7 ℃ of conditions, gets the outer dialyzate of 100L bag, and is for subsequent use;
Dialysis liquid cooling freeze-drying is dry outside E, the bag: at-30 ℃, under the vacuum tightness 0.4Pa condition, lyophilize gets lyophilized powder 2.56kg, and is for subsequent use with the outer dialyzate of bag for subsequent use;
F, chromatography: lyophilized powder for subsequent use is dissolved into the solution of 10mg/ml, crosses Sephadex G-25 dextrane gel, get the chromatographic solution 20L that automatic Fraction Collector is collected, for subsequent use;
G, chromatographic solution lyophilize: chromatographic solution for subsequent use is carried out lyophilize under-30 ℃, vacuum tightness 0.4Pa condition, get 2.4kg lyophilize powder, be antioxidation polypeptide.
Embodiment three:
A, the bent preparation of kind: get a meter slag and (produce the gained by product through organic acid, antibiotic fermentation and β-amylose, lower same), be modulated into water content after the boiling and be 65% kind koji fermentation material 0.25kg, add food grade fermented bacterium aspergillus oryzae again, the spore inoculating amount is by 8 * 10
6Individual/gram calculates, and is 2 * 10
9Individual spore is mixed all, places under humidity 85%, 28 ℃ of conditions of temperature, and fermentation culture 4 days gets the bent material of 0.25kg kind, and is for subsequent use;
B, primary fermentation: other gets and is modulated into water content after meter slag boiling is that 5kg is expected in 65% fermentation, adds the bent material of kind of 0.25kg again, mixes thoroughly, places under humidity 85%, 28 ℃ of conditions of temperature, and fermentation culture 4 days gets 5.25kg primary fermentation material, and is for subsequent use;
C, secondary fermentation: 5.25kg primary fermentation material for subsequent use is dropped in the 52.5L water, mix all; Then, under 41 ℃ of conditions, carry out secondary fermentation 6 days, get 57.75kg secondary fermentation material, for subsequent use;
D, dialysis: place 20KD to hold back dialysis tubing in 57.75kg secondary fermentation material for subsequent use, dialysis is 18 hours under 7 ℃ of conditions, gets the outer dialyzate of 160L bag, and is for subsequent use;
Dialysis liquid cooling freeze-drying is dry outside E, the bag: at-45 ℃, under the vacuum tightness 200Pa condition, lyophilize gets lyophilized powder 2.66kg, and is for subsequent use with the outer dialyzate of bag for subsequent use;
F, chromatography: lyophilized powder for subsequent use is dissolved into the solution of 30mg/ml, crosses Sephadex G-25 dextrane gel, get the chromatographic solution 35L that automatic Fraction Collector is collected, for subsequent use;
G, chromatographic solution lyophilize: chromatographic solution for subsequent use is carried out lyophilize under-45 ℃, vacuum tightness 200Pa condition, get 2.5kg lyophilize powder, be antioxidation polypeptide.
Following table be three embodiment when producing desired value and the main detection index of antioxidation polypeptide:
A method of utilizing the rice slag to produce antioxidation polypeptide of the present invention, both applicable rice slag was made raw material, and also applicable other sedimentable protein is made raw material, carries out antioxidation polypeptide production.
Claims (1)
1. one kind is utilized meter method of slag production antioxidation polypeptide, it is characterized in that technological process is as follows:
A, the bent preparation of kind: will be modulated into the kind koji fermentation material that water content is 45-65% after the boiling of rice slag, add again food grade fermented bacterium aspergillus oryzae, its spore inoculating amount is 2-8 * 106/gram, mix and all be placed under humidity 80-95%, the temperature 25-32 ℃ condition, fermentation culture 3-5 days, become kind of a bent material, for subsequent use;
B, primary fermentation: other is modulated into the fermentation expectation that water content is 45-65% after getting meter slag boiling, adds the kind song that accounting for fermentation expectation weight 1-10% again and expects, mixes thoroughly, place under humidity 80-95%, the temperature 25-32 ℃ condition, fermentation culture 3-5 days, become the primary fermentation material, for subsequent use;
C, secondary fermentation: primary fermentation material for subsequent use is dropped in the sterilized water, and the consumption of its water adds water 60-100ml according to every 10g primary fermentation material and calculates; Then, under 40-42 ℃ of condition, carried out secondary fermentation 5-7 days, become the secondary fermentation material, for subsequent use;
D, dialysis: place 20KD to hold back dialysis tubing in secondary fermentation material for subsequent use, dialysis is 12-24 hour under 3-10 ℃ of condition, and the outer dialyzate of bag taking is for subsequent use;
Dialysis liquid cooling freeze-drying is dry outside E, the bag: the outer dialyzate of bag for subsequent use is carried out lyophilize, and condition is :-30--45 ℃, vacuum tightness 0.4-200Pa gets lyophilized powder, and is for subsequent use;
F, chromatography: lyophilized powder for subsequent use is made into the solution of 10-30mg/ml, crosses Sephadex G-25 dextrane gel, get the chromatographic solution that automatic Fraction Collector is collected, for subsequent use;
G, chromatographic solution lyophilize: chromatographic solution for subsequent use is carried out lyophilize, and condition is :-30--45 ℃, and vacuum tightness 0.4-200Pa, the gained powder is antioxidation polypeptide.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210336484.8A CN102864199B (en) | 2012-09-08 | 2012-09-08 | Method for producing anti-oxidative polypeptide by using rice residue |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210336484.8A CN102864199B (en) | 2012-09-08 | 2012-09-08 | Method for producing anti-oxidative polypeptide by using rice residue |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102864199A true CN102864199A (en) | 2013-01-09 |
| CN102864199B CN102864199B (en) | 2014-06-04 |
Family
ID=47443325
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210336484.8A Expired - Fee Related CN102864199B (en) | 2012-09-08 | 2012-09-08 | Method for producing anti-oxidative polypeptide by using rice residue |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102864199B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105821101A (en) * | 2016-06-01 | 2016-08-03 | 中南林业科技大学 | Method for fermentation and preparation of rice residue protein antioxidant active peptides |
| CN106011205A (en) * | 2016-05-16 | 2016-10-12 | 青岛海和创兴海洋生物科技有限公司 | Fermented type polypeptide biological base material |
| CN115261170A (en) * | 2022-09-05 | 2022-11-01 | 惠风酒业发展(上海)有限公司 | Distiller's yeast and health rice wine |
| CN115477985A (en) * | 2022-10-17 | 2022-12-16 | 惠风酒业发展(上海)有限公司 | Health-care rice wine and preparation method thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1379089A (en) * | 2001-03-29 | 2002-11-13 | 科学工业研究院 | Method of preparing deactivated tikitiki lipase |
-
2012
- 2012-09-08 CN CN201210336484.8A patent/CN102864199B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1379089A (en) * | 2001-03-29 | 2002-11-13 | 科学工业研究院 | Method of preparing deactivated tikitiki lipase |
Non-Patent Citations (2)
| Title |
|---|
| WEI TIAN ET AL.: "In vitro antioxidant capacities of rice residue hydrolysates from fermented broth of five mold strains", 《JOURNAL OF MEDICINAL PLANTS RESEARCH》 * |
| 汪兴平: "葛仙米藻蓝蛋白的一级结构、构象及抗氧化活性研究", 《中国博士论文全文数据库（工程科技I辑）》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106011205A (en) * | 2016-05-16 | 2016-10-12 | 青岛海和创兴海洋生物科技有限公司 | Fermented type polypeptide biological base material |
| CN105821101A (en) * | 2016-06-01 | 2016-08-03 | 中南林业科技大学 | Method for fermentation and preparation of rice residue protein antioxidant active peptides |
| CN115261170A (en) * | 2022-09-05 | 2022-11-01 | 惠风酒业发展(上海)有限公司 | Distiller's yeast and health rice wine |
| CN115477985A (en) * | 2022-10-17 | 2022-12-16 | 惠风酒业发展(上海)有限公司 | Health-care rice wine and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102864199B (en) | 2014-06-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106244658B (en) | Preparation method of sweet potato protein polypeptide | |
| CN104513748A (en) | Method for making kvass beverage from germinated brown rice | |
| CN102318724B (en) | Production method of acid soybean peptide protein | |
| CN103194512B (en) | Soybean peptide for promoting lactobacillus proliferation and improving lactobacillus survivability as well as preparation method and application thereof | |
| CN102864199B (en) | Method for producing anti-oxidative polypeptide by using rice residue | |
| CN102660622A (en) | Method for preparing bioactive soybean peptide by mixed culture solid-state fermentation | |
| CN101766282A (en) | Process method for simultaneously extracting soybean function factor and therapeutic factor from bean pulp | |
| CN106565838B (en) | Phycocyanin with good stability, and preparation method, composition and application thereof | |
| CN101126103A (en) | Method for preparing corn protein peptide with high F valve by microorganism zymotechnics | |
| CN103070431B (en) | Kelp extract iodine tablet and preparation method thereof | |
| CN103789383A (en) | Rice peptide production method | |
| CN104996715A (en) | A processing method for preparing wheat germ polypeptide by a compound fermentation method | |
| CN102199647A (en) | Method for separation and extraction of active peptide from domestic fungus by-products | |
| KR101377586B1 (en) | Method for producing fermented inonotus obliquus with increased antioxidant activity and total phenolic compounds by the solid-state fermentation | |
| CN102228125A (en) | Preparation method of algal active peptide | |
| CN109170922B (en) | Preparation method of wheat bran soluble dietary fiber | |
| CN104087637A (en) | Method for preparing vegetable protein polypeptide by virtue of microbiological fermentation | |
| CN104152522A (en) | Method for preparing wheat active peptide from mixed bacterium solid state fermentation gluten powder | |
| CN109880874A (en) | A kind of preparation method of rice active peptide, rice active peptide and its application | |
| CN101869265B (en) | Method for using enzyme method for assisting aqueous extraction of insoluble dietary fiber from pollen pini | |
| CN106591078A (en) | Preparation method of black rice vinegar | |
| CN105483177B (en) | Preparation method of water-insoluble exopolysaccharide of leuconostoc mesenteroides | |
| CN104557154A (en) | Method for producing amino acid fertilizer from soybean meal through solid-state fermentation | |
| KR20070111874A (en) | Manufacturing method for extract of chlorella | |
| CN105420311A (en) | Novel technology for producing extracellular polysaccharides and fructose by means of litchi juice or longan juice fermentation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140604 Termination date: 20160908 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |