CN106011205A - Fermented type polypeptide biological base material - Google Patents

Fermented type polypeptide biological base material Download PDF

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Publication number
CN106011205A
CN106011205A CN201610320408.6A CN201610320408A CN106011205A CN 106011205 A CN106011205 A CN 106011205A CN 201610320408 A CN201610320408 A CN 201610320408A CN 106011205 A CN106011205 A CN 106011205A
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CN
China
Prior art keywords
biological base
fermented type
fermentation
type polypeptide
polypeptide
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
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CN201610320408.6A
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Chinese (zh)
Inventor
刘尊英
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Sea Of Qingdao And Chong Hing Marine Biotechnology Co Ltd
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Sea Of Qingdao And Chong Hing Marine Biotechnology Co Ltd
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Application filed by Sea Of Qingdao And Chong Hing Marine Biotechnology Co Ltd filed Critical Sea Of Qingdao And Chong Hing Marine Biotechnology Co Ltd
Priority to CN201610320408.6A priority Critical patent/CN106011205A/en
Publication of CN106011205A publication Critical patent/CN106011205A/en
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Zoology (AREA)
  • Water Supply & Treatment (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The invention relates to a fermented type polypeptide biological base material and belongs to the technical field of food deep processing. The fermented type polypeptide biological base material is characterized in that side products such as fish skin and fish bone in aquatic product processing serve as raw materials, and the fermented type polypeptide biological base material high in hydrolysis degree and stability is prepared through the procedures of starter propagation, fermentation, polypeptide separation, drying and packaging. The technology does not need expensive or complex equipment, is low in production cost and is a biotransformation processing technology, and industrialized production can be easily achieved; the fermented type polypeptide produced through the technology is high in solubility and good in foaming characteristic and emulsifying property and has a certain anti-oxidation activity, and the polypeptide biological base material has wide market popularization prospects.

Description

A kind of fermented type polypeptide biological base
Technical field
The invention belongs to technical field of food deep processing, be specifically related to a kind of fermented type polypeptide biological base product.
Background technology
Polypeptide is the compounds between amino acid and protein.The familiar insulin of people is exactly a kind of Polypeptide.The basic group of composition polypeptide is aminoacid, and in general, the polypeptide being made up of 3 aminoacid is tripeptides, by 4 groups Become is tetrapeptide, analogizes along this.It has been found that polypeptides matter is in addition to having the Nutrition of general protein, also have Multiple biological activity is thin including antioxidant activity, hypolipidemic activity, hypotensive activity, enhancing immunity, resisting fatigue and promotion The effects such as born of the same parents' propagation.
The preparation method of biologically active peptide has extracting directly, chemosynthesis, DNA recombinant technique and enzymolysis to prepare at present. But because the abundance in vivo of natural activity peptide is relatively low, and the reason such as raw material sources, cause direct extraction method This is high；Chemical method is mainly used to synthesize the polypeptide of the moderate-length with higher pharmaceutical value, but can have with some The residual of pest matter or generation；And it is only suitable for synthesizing the bigger albumen of number molecular weight or macromole peptide with DNA recombinant technique, its Cost is the highest；Enzymolysis preparation easily realizes industrialization, and its shortcoming is that product composition is complicated, and single component purity is the highest.
Summary of the invention
It is an object of the invention to provide a kind of fermented biological polypeptide, thus a kind of good stability, preparation method letter are provided Single fermented type polypeptide products, to make up the deficiency of existing polypeptide products.
A kind of fermented type polypeptide biological base, it is characterised in that described polypeptide is to be prepared with following steps:
1) yeast production: carry out yeast production for strain with aspergillus oryzae, aspergillus oryzae is inoculated into containing bean cake, wheat bran culture medium in, inoculation Amount is 107Individual/g, carried out turning over song every 6-12 hour so that it is spore count controls in 1-2 × 1010Individual/g；
2) fermentation: by fish skin fishbone serosity that quality percent by volume is 10%, the sucrose of 0.5%, 0.1%K2HPO4, 0.1% MgSO4After fermentation liquid sterilizing, inoculation step 1) in the aspergillus oryzae for preparing, 30 DEG C of cultivations, treat that fish skin fishbone degree of hydrolysis is at 30%-35% Time stop fermentation；
3) peptide separation: step 2) in fermentation liquid be that 1000 daltonian ultrafilter membranes carry out ultrafiltration by molecular retention amount, filter Liquid is fermentation polypeptide solution；
4) it is dried: the fermentation polypeptide solution in step 3) is carried out cryoconcentration, then carries out lyophilization or cold nebulization is done Dry, and add the mannitol protective agent that mass ratio is 1%-5% before it is dried；
5) packaging: dried polypeptide extract is fermented type polypeptide biological base after vacuum packaging.
Preferably, koji-making condition is that bean cake wheat bran controls at 33 DEG C-35 DEG C than 1:2, water content 35%-50%, temperature, cultivates Base thickness is set to 1cm-3cm.
Preferably, the polypeptide biological base molecular weight of the present invention is below 1000 dalton.
The fermented type polypeptide biological base preparation technology of this invention is simple, it is easy to industrialization, and reaction condition is gentle, is a kind of Bioconversion process technology.Aspergillus oryzae is fermented bacterium conventional in food industry, its meeting under conditions of nitrogen source nutrition hunger Self rising in value and secrete a large amount of protease and the protein that decomposes in culture medium produces bioactive peptide, albumen conversion ratio is high, product matter Measure, overcome a large amount of obstacles using industrial enzyme preparation cost high in the preparation of existing enzymolysis.
Accompanying drawing explanation
Fig. 1: the product of the present invention dissolubility under condition of different pH.
Fig. 2: the emulsibility of product of the present invention and emulsion stability figure.
Detailed description of the invention
The present invention is further illustrated below in conjunction with detailed description of the invention.
Embodiment 1: a kind of fermented type polypeptide biological base, concrete preparation process is as follows:
1) yeast production: carry out yeast production for strain with aspergillus oryzae, aspergillus oryzae is inoculated into containing bean cake, wheat bran culture medium in, inoculation Amount is 107Individual/g, carried out turning over song every 12 hours so that it is spore count controls 1.25 × 1010Individual/g；
2) fermentation: by fish skin fishbone serosity that quality percent by volume is 10%, the sucrose of 0.5%, 0.1% K2HPO4, 0.1% MgSO4After fermentation liquid sterilizing, inoculation step 1) in the aspergillus oryzae for preparing, 30 DEG C of cultivations, stop when fish skin fishbone degree of hydrolysis is 35% Only fermentation；
3) peptide separation: step 2) in fermentation liquid be that 1000 daltonian ultrafilter membranes carry out ultrafiltration by molecular retention amount, filter Liquid is fermentation polypeptide solution；
4) it is dried: the fermentation polypeptide solution in step 3) is carried out cryoconcentration, then carries out lyophilization or cold nebulization is done Dry, and add the mannitol protective agent that mass ratio is 5% before it is dried；
5) packaging: dried polypeptide extract is fermented type polypeptide biological base after vacuum packaging.
Fermented type polypeptide base material prepared by the present invention, dissolubility is good, and under pH neutrallty condition, its dissolubility is more than more than 95% (Fig. 1)；The emulsibility of product and emulsion stability are high, when fermentation peptide mass volume ratio concentration is more than 0.2%, and its emulsion stability Close to 70%(Fig. 2).The fermentation peptide product of the present invention also has the activity of certain antioxidation and blood pressure lowering, with DPPH and blood vessel Angiotensin-converting enzyme is index, and its DPPH free radical suppression ratio reaches as high as 63%, the IC50 of its angiotensin converting enzyme suppression Value is 2.53 mg/ml.
The bioactive peptide of gained of the present invention not only dissolubility is high, and emulsibility, foaming characteristic are good, and have certain antioxidation and Hypotensive activity, is the polypeptide biological base of a kind of great promotion prospect.

Claims (3)

1. a fermented type polypeptide biological base, it is characterised in that described polypeptide is to be prepared with following steps:
1) yeast production: carry out yeast production for strain with aspergillus oryzae, aspergillus oryzae is inoculated into containing bean cake, wheat bran culture medium in, inoculation Amount is 107/g, carries out turning over song every 6-12 hour so that it is spore count controls in 1-2 × 1010/g；
2) fermentation: by fish skin fishbone serosity that quality percent by volume is 10%, the sucrose of 0.5%, 0.1%K2HPO4,0.1% After MgSO4 fermentation liquid sterilizing, inoculation step 1) in the aspergillus oryzae for preparing, 30 DEG C of cultivations, treat that fish skin fishbone degree of hydrolysis is at 30%-35% Time stop fermentation；
3) peptide separation: step 2) in fermentation liquid be that 1000 daltonian ultrafilter membranes carry out ultrafiltration by molecular retention amount, filter Liquid is fermentation polypeptide solution；
4) it is dried: the fermentation polypeptide solution in step 3) is carried out cryoconcentration, then carries out lyophilization or cold nebulization is done Dry, and add the mannitol protective agent that mass ratio is 1%-5% before it is dried；
5) packaging: dried polypeptide extract is fermented type polypeptide biological base after vacuum packaging.
2. fermented type polypeptide biological base as claimed in claim 1, it is characterised in that koji-making condition be bean cake wheat bran than 1:2, Water content 35%-50%, temperature controls at 33 DEG C-35 DEG C, and culture medium thickness is set to 1cm-3cm.
3. fermented type polypeptide biological base as claimed in claim 1, it is characterised in that described polypeptide biological base molecular weight It is below 1000 dalton.
CN201610320408.6A 2016-05-16 2016-05-16 Fermented type polypeptide biological base material Pending CN106011205A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201610320408.6A CN106011205A (en) 2016-05-16 2016-05-16 Fermented type polypeptide biological base material

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201610320408.6A CN106011205A (en) 2016-05-16 2016-05-16 Fermented type polypeptide biological base material

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CN106011205A true CN106011205A (en) 2016-10-12

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106538845A (en) * 2016-11-26 2017-03-29 青岛中泰和生物科技有限公司 A kind of chelating ferrous preparation method of marine protein peptide
CN106538844A (en) * 2016-11-26 2017-03-29 青岛中泰和生物科技有限公司 A kind of preparation method of marine protein peptide chelated copper

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102251002A (en) * 2011-06-23 2011-11-23 淮海工学院 Method for preparing antioxidant polypeptide by solid fermentation of acanthogobius hasta muscle protein
CN102864199A (en) * 2012-09-08 2013-01-09 长沙帮得生物科技有限公司 Method for producing anti-oxidative polypeptide by using rice residue
CN104356203A (en) * 2014-11-12 2015-02-18 中国农业大学 Antioxidant polypeptide as well as preparation method and application thereof
CN105506039A (en) * 2016-02-01 2016-04-20 浙江工业大学 Method for preparing anti-oxidative peptide from aspergillus oryzae fermented shrimp shells

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102251002A (en) * 2011-06-23 2011-11-23 淮海工学院 Method for preparing antioxidant polypeptide by solid fermentation of acanthogobius hasta muscle protein
CN102864199A (en) * 2012-09-08 2013-01-09 长沙帮得生物科技有限公司 Method for producing anti-oxidative polypeptide by using rice residue
CN104356203A (en) * 2014-11-12 2015-02-18 中国农业大学 Antioxidant polypeptide as well as preparation method and application thereof
CN105506039A (en) * 2016-02-01 2016-04-20 浙江工业大学 Method for preparing anti-oxidative peptide from aspergillus oryzae fermented shrimp shells

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
吴海滨等: "米曲霉发酵鳕鱼皮制取低分子肽条件的优化及活性研究", 《食品与发酵工业》 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106538845A (en) * 2016-11-26 2017-03-29 青岛中泰和生物科技有限公司 A kind of chelating ferrous preparation method of marine protein peptide
CN106538844A (en) * 2016-11-26 2017-03-29 青岛中泰和生物科技有限公司 A kind of preparation method of marine protein peptide chelated copper
CN106538844B (en) * 2016-11-26 2021-01-01 青岛海大生物集团有限公司 Preparation method of marine organism protein peptide chelated copper

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