CN102864119B - Carrier for cell culturing and preparation method of carrier - Google Patents
Carrier for cell culturing and preparation method of carrier Download PDFInfo
- Publication number
- CN102864119B CN102864119B CN2012103644209A CN201210364420A CN102864119B CN 102864119 B CN102864119 B CN 102864119B CN 2012103644209 A CN2012103644209 A CN 2012103644209A CN 201210364420 A CN201210364420 A CN 201210364420A CN 102864119 B CN102864119 B CN 102864119B
- Authority
- CN
- China
- Prior art keywords
- dimensional porous
- porous graphene
- carrier
- cell
- support
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/14—Scaffolds; Matrices
Landscapes
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Immunology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a carrier for cell culturing. The carrier is a three-dimensional porous graphene support which is fixed on a transparent polystyrene or glass material substrate, liquid tank walls are fixed on the periphery of the three-dimensional porous graphene support to form a culturing tank used for cell culturing. The invention further discloses a preparation method of the carrier for cell culturing. Compared with the prior art, the method includes adopting the three-dimensional porous graphene support infiltrated by solvent before removing a metal catalyst formwork, and removing the air absorbed on the surface of the support by means of vacuum air suction, so that floating of materials on the surface of liquid due to surface tension and density is avoided, and residues of metal and ions can be removed more easily in the process of subsequent treatment. The three-dimensional porous graphene support is used as culturing carrier, realizes three-dimensional culturing of cells, simulates in-vivo growth environment of the cells, is beneficial to maintaining growth states and activity of the cells and accelerates cell growth.
Description
Technical field
The present invention relates to a kind of carrier for cell cultures and preparation method thereof, belong to technical field of biological material.
Background technology
Graphene is a kind of novel carbon nanomaterial, has unique two-dimension plane structure, has been applied to the fields such as flexible and transparent electrode, strongthener; At biomedical sector, Graphene also receives much concern in recent years, has been used to cell imaging, drug delivery, bioanalysis, Stem Cell Engineering and the research of oncotherapy aspect simultaneously.
Spongy three-dimensional porous grapheme material is at first by discoveries such as the Cheng Huiming of Shenyang metal institute, its synthetic method is generally, take nickel foam as catalyzer, organic gas is carbon source as methane, the three-dimensional porous grapheme material that adopts chemical Vapor deposition process (CVD) preparation to contain catalyzer, then obtain spongy three-dimensional porous grapheme material after the erosion removal catalyzer.Grapheme material prepared by this method has good electricity, mechanical property, can further be applied to the biomedical sectors such as biochip, embedded material.
Cell in histoorgan is all grown in three-dimensional environment, therefore on the Tissue Culture Dish of two dimensional surface at present or graphene substrate during culturing cell, be subject to the planar growth environmental limit, the activity of cell, pattern and growth conditions etc. with compared very large change under internal milieu, therefore explore and spongy three-dimensional porous Graphene is applied to cell cultures there is important value; And, because cell cultures has very high requirement to foreign matter content and the course of processing of material, how to prepare there is high conductivity, the three-dimensional porous Graphene of ultralight porous, good biocompatibility is also a difficult problem urgently to be resolved hurrily.
Summary of the invention
The problem existed for the above-mentioned prior art of mentioning, the invention provides a kind of carrier for cell cultures and preparation method thereof, this cell culture vector has been realized the dimensional culture of cell, the tumor growth environment of analog cell, be conducive to maintain growth conditions and the activity of cell, Promote cell's growth.
For achieving the above object, the present invention has adopted following technical scheme:
A kind of carrier for cell cultures, described carrier is three-dimensional porous Graphene support.
Further, the appearance of described three-dimensional porous Graphene support is modified with bioactive molecules, and described bioactive molecules can be poly-lysine or ln.
Preferably, the aperture of described three-dimensional porous Graphene support is the 20-500 micron, and density is 2-20 mg/cm
3.
Described three-dimensional porous Graphene support is fixed in transparent polystyrene or glass material substrate, and around three-dimensional porous Graphene support the stationary liquid pool wall, form cultivation pool, for cell cultures; Institute's cultured cells is at least one or more in tumour cell, primary neurocyte and neural stem cell.
The preparation method of the carrier for cell cultures as above, it comprises the following steps:
(a) the applied chemistry gas-phase deposition prepares three-dimensional porous Graphene support on the metal foam support;
(b) the three-dimensional porous Graphene that will contain the metal foam support is immersed in 5-20 min in the mixed solvent of alcohol and water; Adopt vacuum suction to remove the air of three-dimensional porous Graphene rack surface absorption;
(c) in closed container, adopt with acid etching solution and remove the metal foam support, then adopt successively the hydrochloric acid of 1,0.1,0.01,0.001 mol/L or salpeter solution respectively to wash 1 time, then with deionized water wash for several times; Obtain the carrier for cell cultures: three-dimensional porous Graphene support.
Preferably, after the middle vacuum suction of step (b), pressure is less than 10kPa, and keeps at least 30 minutes.
Preferably, described metal catalyst is nickel foam, foam copper, foam gold copper or nickel foam copper alloy.
Compared with prior art, advantage of the present invention at least is:
(1) cell culture vector provided by the invention, culturing cell on three-dimensional porous Graphene supporting structure, because the vesicular structure of three-dimensional porous grapheme material has the superelevation porosity, for Growth of Cells provides adequate space, realize the dimensional culture of cell, simulate the tumor growth environment of cell, be conducive to maintain growth conditions and the activity of cell, Promote cell's growth; And can keep the good mechanics of intrinsic Graphene, electricity, chemical property through the three-dimensional porous Graphene of present method processing, meet the demands such as regulating cell growth and differentiation;
(2) the present invention is in the process for preparing the three-dimensional porous Graphene support that is used as substrate, before removing the catalyzer template, at first adopt solvent to infiltrate three-dimensional porous Graphene support, avoid material to swim in fluid surface because of the reason of surface tension and density, and vacuumize the air of removing material surface absorption, improve the wetting property of corrosive fluid, and carry out immersion treatment in enclosed environment, effectively avoided the volatilization of corrosive fluid and condensed, more easily in subsequent processes, remove the remnants of metal and ion, thereby significantly improve the biocompatibility of material and the repeatability for the treatment of processes, effectively avoid catalyzer and etching reagent to remain in the Graphene support, improved activity and the survival rate of culturing cell.
The accompanying drawing explanation
In order to be illustrated more clearly in the embodiment of the present application or technical scheme of the prior art, below will the accompanying drawing of required use in embodiment or description of the Prior Art be briefly described, apparently, the accompanying drawing the following describes is only some embodiment that put down in writing in the application, for those of ordinary skills, under the prerequisite of not paying creative work, can also obtain according to these accompanying drawings other accompanying drawing.
Fig. 1 is the structural representation that contains the cultivation pool of three-dimensional porous Graphene support in a preferred embodiment of the present invention;
Fig. 2 is that the three-dimensional porous Graphene rack surface in the embodiment of the present invention is cultivated PC 12 cells, the fluorescence photo after 'beta '-tubulin (Tubulin) immunofluorescence dyeing;
Fig. 3 is the stereoscan photograph of three-dimensional porous Graphene support in the embodiment of the present invention.
Embodiment
For shortcomings and deficiencies of the prior art, through studying for a long period of time and put into practice, the present invention proposes, utilize three-dimensional porous Graphene support as the conception of cell culture vector and the preparation method of this carrier for cell cultures.
Should be three-dimensional porous Graphene support 2 for the carrier of cell cultures; The appearance of described three-dimensional porous Graphene support 2 can be modified with bioactive molecules, and described bioactive molecules can be poly-lysine or ln; The aperture of described three-dimensional porous Graphene support 2 is the 20-500 micron, and density is 2-20 mg/cm
3.
As shown in Figure 1, described three-dimensional porous Graphene support 2 is fixed in transparent polystyrene or glass material substrate 1, and around three-dimensional porous Graphene support stationary liquid pool wall 4, form cultivation pool; Then adopt acetone, Virahol, alcohol to clean and remove organic substance residues, then soak three-dimensional porous Graphene support 2 further to remove the solubility toxic substance with a large amount of deionized waters; Inject substratum and form liquid pool 3 in cultivation pool, for cell cultures; Institute's cultured cells is at least one or more in tumour cell, primary neurocyte and neural stem cell.
The present invention also provides the preparation method of a kind of making for the carrier of cell cultures, at first it mainly comprise the following steps:, adopt the method for traditional C VD, it is catalyzer that the porosity of take is about 95% metal foam, under 1000 ℃ of conditions, take methane as carbon source prepares individual layer or few layer graphene, be prepared with the three-dimensional porous Graphene support of metal foam; Secondly, the three-dimensional porous Graphene that will contain metal foam is immersed in 5-20 min in the mixed solvent of alcohol and water, adopts vacuum suction to remove the air of three-dimensional porous Graphene rack surface absorption; Then, in closed container, adopt and remove metal foam with acid etching solution, finally adopt successively the hydrochloric acid of 1,0.1,0.01,0.001 mol/L or salpeter solution respectively to wash once, then obtain for several times three-dimensional porous Graphene support with deionized water wash.Wherein, the aperture of three-dimensional porous Graphene support is by the aperture structure decision of nickel foam, and density is relevant with the number of plies of material.Preferably, the three-dimensional porous Graphene support number of plies is five to ten layers, and aperture is between the 100-300 micron, and density is 2-20 mg/cm in density
3.
By the present invention, can realize the dimensional culture of various types of cells on the Graphene surface, for further graphene-based biomedical applications provides technical support simultaneously.
Below in conjunction with some preferred embodiments, technical scheme of the present invention is described further.
Adopt the method for CVD, take porosity 95 %, aperture is catalyzer in the nickel foam of 30-300 micron, under 900 ℃ of conditions, take methane as carbon source for growth 5-10 layer graphene.The three-dimensional porous Graphene support that will contain catalyzer nickel is immersed in containing 10 min in the aqueous solution of 75 % alcohol, bleeds and makes pressure be less than 10 kPa, keeps 30 min, removes the air of three-dimensional porous Graphene rack surface absorption; Then pass into air to normal pressure, add the acidic ferric chloride corrosive fluid of 1000 times of volumes, soak 24 h to remove catalyzer nickel in sealed environment; Then adopt successively the hydrochloric acid soln of 1,0.1,0.01,0.001 mol/L respectively to wash 1 time, then use deionized water wash 3 times.The dry back aperture of the three-dimensional porous Graphene support made, between the 20-300 micron, slightly reduces than the aperture of nickel foam template, and density is about 4-5 mg/cm
3.
By substrate of glass, three-dimensional porous Graphene support is picked up, directly adopts DOW CORNING 3140 silicone adhesive agents to fix, by the liquid pool wall be fixed in three-dimensional porous Graphene support around, and at ambient temperature set times 12 h, form cultivation pool; Then use acetone treatment 5 h, clean 0.5 h with Virahol subsequently, then use a large amount of washed with de-ionized water, and soak 120 h.The carrier for cell cultures made is soaked in to poly-lysine PBS solution 12 h of 1 mg/mL, then rinses with a large amount of sterilizing PBS damping fluids.
Inject containing 15% blood serum medium in cultivation pool, and soak 2 h, for the cultivation of PC12 cell (the adult rat adrenal tissue medullary substance is had a liking for the strain of chromium knurl noble cells), substratum is the DMEM containing 15 % foetal calf serums, and inoculum density is 10
5cell/cm
2.Cultivation results shows that the cell cell space of growth is plentiful, endochylema is transparent, the cell edges refractive index is high, and growth conditions is good, as shown in Figure 2.
Adopt the method for CVD, take porosity 95 %, aperture is catalyzer at the foam copper of 100-300 micron, under 950 ℃ of conditions, take methane as carbon source for growth 10-20 layer graphene.The three-dimensional porous Graphene support that will contain catalyzer nickel is immersed in containing 20 min in the aqueous solution of 98 % alcohol, bleeds and makes pressure to 100 Pa, keeps 30 min, removes the air of three-dimensional porous Graphene rack surface absorption; Then pass into air to normal pressure, add the acid etching solution of 100 times of volumes to remove catalyzer nickel; Then adopt successively the salpeter solution of 1,0.1,0.01 mol/L respectively to wash 1 time, then use deionized water wash 6 times.The dry back aperture of the three-dimensional porous Graphene support made is between the 100-300 micron, and density is about 5-8 mg/cm
3, pattern as shown in Figure 3.
By substrate of glass, three-dimensional porous graphite support alkene is picked up, directly adopts DOW CORNING 3140 silicone adhesive agents to fix after dry, by the liquid pool wall be fixed in three-dimensional porous Graphene support around, and at ambient temperature set time 24h, form cultivation pool; Then use acetone treatment 2 h, clean 2 h with alcohol subsequently, then use a large amount of washed with de-ionized water, and soak 24 h.The carrier for cell cultures made is soaked in to poly-lysine PBS solution 12 h of 1 mg/mL, then rinses with a large amount of sterilizing PBS damping fluids; Inject the DMEM substratum containing 15% serum in cultivation pool, can be used for the PC12 cell cultures.
Adopt the method for CVD, take porosity 95 %, aperture is catalyzer at the foam gold copper of 300-500 micron, under 750 ℃ of conditions, take methane as carbon source for growth 50-100 layer graphene.The three-dimensional porous Graphene support that will contain catalyzer nickel is immersed in containing 60 min in the aqueous solution of 2 % alcohol, bleeds and makes pressure be less than 10 kPa, keeps 30 min, removes the air of three-dimensional porous Graphene rack surface absorption; Then pass into air to normal pressure, add the acidic ferric chloride corrosive fluid of 1000 times of volumes to remove catalyzer nickel; Then adopt successively 1,0.1,0.01,0.001, the hydrochloric acid soln of 0.0001 mol/L respectively washs 1 time, then uses deionized water wash 3 times.The dry back aperture of the three-dimensional porous Graphene support made is between the 300-500 micron, and density is about 15-20 mg/cm
3.
Polystyrene (TCPS) substrate that using-system is cultivated purposes picks up three-dimensional porous Graphene, directly adopt DOW CORNING 3140 silicone adhesive agents to fix, by the liquid pool wall be fixed in three-dimensional porous Graphene support around, and at ambient temperature set time 48h, form cultivation pool; Then process 5 h with alcohol, then use a large amount of washed with de-ionized water, and soak 1 h.The carrier for cell cultures made is soaked in to poly-lysine PBS solution 24 h of 10 mg/mL, then rinses with a large amount of sterilizing PBS damping fluids.
Inject containing 15% blood serum medium in cultivation pool, and soak 2 h, for the cultivation of primary hippocampal neurons, substratum is the DMEM containing 15% foetal calf serum, and inoculum density is 10
6cell/cm
2.Cultivation results shows that the biocompatibility of three-dimensional porous Graphene support is good, cell growth state, the demonstration of MTT detected result, and cell growth state all is better than its growth conditions on two dimensional surface Graphene and TCPS substrate, and cell survival rate improves 50 %.
Adopt the method for CVD, take porosity 95 %, aperture is catalyzer at the foam copper nickelalloy of 100-300 micron, under 900 ℃ of conditions, take methane as carbon source for growth 1-5 layer graphene.The three-dimensional porous Graphene support that will contain catalyzer nickel is immersed in containing 5 min in the aqueous solution of 50 % alcohol, bleeds and makes pressure be less than 100 Pa, keeps 30 min, removes the air of three-dimensional porous Graphene rack surface absorption; Then pass into air to normal pressure, add the acid iron nitrate corrosive fluid of 100 times of volumes to remove catalyzer nickel; Then adopt successively the salpeter solution of 0.1,0.01 mol/L respectively to wash 1 time, then use deionized water wash 3 times.The dry back aperture of the three-dimensional porous Graphene support made, between the 50-300 micron, slightly reduces than the aperture of nickel foam template, and density is about 2-3 mg/cm
3.
With the TCPS substrate, three-dimensional porous Graphene support is picked up, directly adopts DOW CORNING 3140 silicone adhesive agents to fix, by the liquid pool wall be fixed in three-dimensional porous Graphene support around, and at ambient temperature set time 24h, form cultivation pool; Then use acetone treatment 5 h, then use a large amount of washed with de-ionized water, and soak 48 h.The carrier for cell cultures made is soaked in to poly-lysine PBS solution 12 h of 1 mg/mL, then rinses with a large amount of sterilizing PBS damping fluids.Inject containing 15% blood serum medium in cultivation pool, and soak 10 h, for the cultivation of primary hippocampal neurons.
embodiment 5
Adopt the method for CVD, take porosity 95 %, aperture is catalyzer in the nickel foam of 100-300 micron, under 900 ℃ of conditions, take methane as carbon source for growth 5-10 layer graphene.The three-dimensional porous Graphene support that will contain catalyzer nickel is immersed in containing 20 min in the aqueous solution of 75 % alcohol, bleeds and makes pressure be less than 10 kPa, keeps 50 min, removes the air of three-dimensional porous Graphene rack surface absorption; Then pass into air to normal pressure, add the acidic ferric chloride corrosive fluid of 1000 times of volumes, soak 24 h to remove catalyzer nickel in sealed vessel; Then adopt successively the hydrochloric acid soln of 1,0.1,0.01,0.001 mol/L respectively to wash 1 time, then use deionized water wash 6 times.The dry back aperture of the three-dimensional porous Graphene support made, between the 100-300 micron, slightly reduces than the aperture of nickel foam template, and density is about 4-5 mg/cm
3.
By substrate of glass, three-dimensional porous Graphene is picked up, directly adopts DOW CORNING 3140 silicone adhesive agents to fix, by the liquid pool wall be fixed in three-dimensional porous Graphene support around, and at ambient temperature set times 12 h, form cultivation pool; Then respectively process 1 h with acetone, Virahol and alcohol, then use a large amount of washed with de-ionized water, and soak 120 h.Adopt biocompatible molecular layer Fibronectin 0.1 μ g/mL to modify 120 h on the three-dimensional porous Graphene support in cultivation pool, then rinse with a large amount of sterilizing PBS damping fluids.
Adopt the Screening of Media method of selecting, cultivate and obtain neural ball sample mouse neural stem cell, adopt the method for unicellular dispersion at three-dimensional porous Graphene rack surface inoculating cell, density is 10
4cell/cm
2.MTT and LDH enzymic activity detected result show two days later, this three-dimensional porous Graphene carrier has good biocompatibility, with the TCPS(control group) compare, survival rate is 98% of control group absorbance value, the LDH analytical results is 101% of control group fluorescent intensity, and the relative cell survival rate of surface both, film integrality are without statistically-significant difference; And before removing the catalyzer template, do not adopt solvent to infiltrate three-dimensional porous Graphene support, and for～40%, LDH enzymic activity (being inversely proportional to cell membrane integrity), be only～400% without vacuumizing the standby three-dimensional grapheme substrate surface cell survival rate of air-making of removing material surface absorption.
embodiment 6
Adopt the method for CVD, take porosity 90 %, aperture is catalyzer in the nickel foam of 100-400 micron, under 900 ℃ of conditions, take methane as carbon source for growth 10-20 layer graphene.The three-dimensional porous Graphene that will contain catalyzer nickel is immersed in containing 20 min in the aqueous solution of 75 % alcohol, bleeds and makes pressure be less than 100 Pa, keeps 30 min, removes the air of three-dimensional porous Graphene rack surface absorption; Then pass into air to normal pressure, add acidic ferric chloride (1 mol/L) solution of 1000 times of volumes, in closed environment, corrosion 120 h remove catalyzer nickel; Then adopt successively the hydrochloric acid soln of 1,0.1,0.01,0.001 mol/L respectively to wash 1 time, then use deionized water wash 6 times.The dry back aperture of the three-dimensional porous Graphene support made is between the 50-300 micron, and density is about 4-6 mg/cm
3.
By substrate of glass, three-dimensional porous Graphene is picked up, directly adopts DOW CORNING 3140 silicone adhesive agents to fix, by the liquid pool wall be fixed in three-dimensional porous Graphene support around, and at ambient temperature set times 12 h, form cultivation pool; Then respectively process 2 h with acetone, Virahol and alcohol, then use a large amount of washed with de-ionized water, and soak 120 h.Inject the DMEM substratum containing 10 % serum in cultivation pool, and soak 12 h, for the cultivation of Marrow Mesenchymal Stem Cells.
It should be noted that, in this article, term " comprises ", " comprising " or its any other variant are intended to contain comprising of nonexcludability, thereby make the process, method, article or the equipment that comprise a series of key elements not only comprise those key elements, but also comprise other key elements of clearly not listing, or also be included as the intrinsic key element of this process, method, article or equipment.In the situation that not more restrictions, the key element limited by statement " comprising ... ", and be not precluded within process, method, article or the equipment that comprises described key element and also have other identical element.
The above is only the application's embodiment; it should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the application's principle; can also make some improvements and modifications, these improvements and modifications also should be considered as the application's protection domain.
Claims (2)
1. the preparation method for the carrier of cell cultures, is characterized in that, it comprises the following steps:
(a) the three-dimensional porous Graphene support that preparation contains metal foam, described metal foam is nickel foam, foam copper, foam gold copper or nickel foam copper alloy;
(b) infiltrate with the mixed solvent of alcohol and water the three-dimensional porous Graphene support that step (a) obtains, adopt vacuum suction to remove the air of three-dimensional porous Graphene rack surface absorption;
(c), in closed container, adopt acid etching solution to remove the metal foam in three-dimensional porous Graphene support.
2. the preparation method of the carrier for cell cultures according to claim 1, is characterized in that, after the middle vacuum suction of step (b), pressure is less than 10kPa, and keeps at least 30 minutes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012103644209A CN102864119B (en) | 2012-09-27 | 2012-09-27 | Carrier for cell culturing and preparation method of carrier |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012103644209A CN102864119B (en) | 2012-09-27 | 2012-09-27 | Carrier for cell culturing and preparation method of carrier |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102864119A CN102864119A (en) | 2013-01-09 |
| CN102864119B true CN102864119B (en) | 2013-12-18 |
Family
ID=47443250
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012103644209A Active CN102864119B (en) | 2012-09-27 | 2012-09-27 | Carrier for cell culturing and preparation method of carrier |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102864119B (en) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104531618B (en) * | 2014-12-21 | 2018-06-26 | 朱熹 | The method that three dimensional growth tumour cell is obtained using graphene nano material |
| CN106701656A (en) * | 2015-07-27 | 2017-05-24 | 天津卫凯生物工程有限公司 | Composite scaffold for cell culture and preparation method thereof |
| CN106701569A (en) * | 2015-08-18 | 2017-05-24 | 重庆润泽医药有限公司 | Tissue cell culture device |
| CN108300713A (en) * | 2017-12-31 | 2018-07-20 | 宁波大学 | The method and device of fixed cell |
| CN109593704B (en) * | 2019-01-31 | 2022-02-11 | 北京华龛生物科技有限公司 | Method for adsorbing and culturing three-dimensional microcarrier cells |
| CN111501350A (en) * | 2020-05-08 | 2020-08-07 | 广东工业大学 | Biological scaffold for inducing growth of nerve cells in vitro and preparation method and application thereof |
| CN114904047B (en) * | 2021-02-07 | 2023-04-07 | 上海大学 | Three-dimensional graphene/extracellular matrix composite scaffold and preparation method and application thereof |
| CN112980689A (en) * | 2021-02-08 | 2021-06-18 | 湖南美柏生物医药有限公司 | Adherent cell culture device, 2.5D beehive type culture system and method |
| CN113184839B (en) * | 2021-05-12 | 2022-11-15 | 沈阳建筑大学 | Cell culture carrier capable of regulating cell growth state |
| CN114134196B (en) * | 2021-12-07 | 2024-06-28 | 浙江大学 | Method and device for simulating toxin to cross blood brain barrier to act on neural stem cells |
| CN114164180A (en) * | 2021-12-08 | 2022-03-11 | 苏州博奥龙科技有限公司 | HAT semi-solid screening culture medium for monoclonal antibody preparation |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012005699A1 (en) * | 2010-07-08 | 2012-01-12 | National University Of Singapore | Method for controlling and accelerating differentiation of stem cells using graphene substrates |
| CN102674321A (en) * | 2011-03-10 | 2012-09-19 | 中国科学院金属研究所 | Graphene foam with three dimensional fully connected network and macroscopic quantity preparation method thereof |
-
2012
- 2012-09-27 CN CN2012103644209A patent/CN102864119B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012005699A1 (en) * | 2010-07-08 | 2012-01-12 | National University Of Singapore | Method for controlling and accelerating differentiation of stem cells using graphene substrates |
| CN102674321A (en) * | 2011-03-10 | 2012-09-19 | 中国科学院金属研究所 | Graphene foam with three dimensional fully connected network and macroscopic quantity preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| Li N. 等.The promotion of neurite sprouting and outgrowth of mouse hippocampal cells in culture by graphene substrates.《Biomaterials》.2011,第9374－9382页. |
| The promotion of neurite sprouting and outgrowth of mouse hippocampal cells in culture by graphene substrates;Li N. 等;《Biomaterials》;20110908;第9374-9382页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102864119A (en) | 2013-01-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102864119B (en) | Carrier for cell culturing and preparation method of carrier | |
| CN102433304B (en) | Graphene substrate for cell culture and preparation method thereof | |
| Gu et al. | The immobilization of hepatocytes on 24 nm-sized gold colloid for enhanced hepatocytes proliferation | |
| CN103954669A (en) | Enzyme electrode, enzyme biosensor as well as preparation methods and application thereof | |
| Li et al. | Vascular lumen simulation and highly-sensitive nitric oxide detection using three-dimensional gelatin chip coupled to TiC/C nanowire arrays microelectrode | |
| CN104046589B (en) | A kind of method of co-culture of cells induction stem cell in vitro directed differentiation | |
| CN102147389A (en) | Method for testing hydrogen peroxide in cell based on horseradish peroxidase-attapulgite nanometer composite material | |
| WO2023019978A1 (en) | Method for preparing mofs-coated myocardial cell core-shell structure | |
| Liu et al. | In vitro mimicking the morphology of hepatic lobule tissue based on Ca-alginate cell sheets | |
| CN106501455A (en) | A kind of preparation method of the highly sensitive stretchable biosensor in situ detection | |
| CN204198745U (en) | A kind of multifunctional unit based on micro-fluidic chip is analyzed porous cell and is cultivated chip | |
| CN109234230A (en) | A kind of primary separation method of skin mesenchymal stem cells | |
| CN108339517A (en) | A kind of flexible biological matter carries selenium material and preparation method thereof | |
| CN107805625A (en) | A kind of graphene culture medium bottom and preparation method thereof and the application in terms of multipotential stem cell cultivation, differentiation and transplanting | |
| CN103525801A (en) | Cell stationary phase based on microcarrier cells and preparation method of cell stationary phase | |
| CN114835847B (en) | Thermosensitive hydrogel as well as preparation method and application thereof | |
| CN110684727A (en) | Zinc oxide nanorod array cell culture substrate and preparation method and application thereof | |
| Haridas et al. | Transfer of printed electronic structures using graphene oxide and gelatin enables reversible and biocompatible interface with living cells | |
| CN202030741U (en) | Culture dish containing micro spherical array | |
| CN204417516U (en) | The special Micro-CPE neutralization test ware of laser scanning co-focusing microscope | |
| CN204211749U (en) | Cell co-culture device | |
| CN111040983A (en) | 3D microcarrier cell adsorption culture method | |
| CN102174381A (en) | Culture dish containing micro spherical arrays and application thereof | |
| Ge et al. | Adhesion-enhanced coral cells with self-healing coating | |
| CN106929838A (en) | The method that preparation is suitable to the enhancing substrate of cell surface bio-orthogonal Raman image |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |