CN102863047A - Method for degradation of microcystin in water by means of electron beam irradiation - Google Patents
Method for degradation of microcystin in water by means of electron beam irradiation Download PDFInfo
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- CN102863047A CN102863047A CN2012103611455A CN201210361145A CN102863047A CN 102863047 A CN102863047 A CN 102863047A CN 2012103611455 A CN2012103611455 A CN 2012103611455A CN 201210361145 A CN201210361145 A CN 201210361145A CN 102863047 A CN102863047 A CN 102863047A
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Abstract
The invention relates to a method for degradation of microcystin in water by means of electron beam irradiation and belongs to the technical field of sewage treatment. The method is a novel technique for microcystin removal in a source water or drinking water treatment procedure. Experimental processes confirm microcystin degradation effect of electron beam irradiation. 2-5kGy irradiation has evident inhibition and removing effects on generation of intracellular toxin, the extracellular toxin removal rates of the 2-5kGy irradiation are 76.9%, 76.2%, 76.2% and 97.7% respectively, and free radicals formed in solution during irradiation are absorbed by algae liquid so that toxins are effectively degraded. Special structures in the toxins are changed or removed by electron beam irradiation, and efficient degradation is achieved. The removal effect is increasingly evident along with increase of dose, and microcystin in algae containing water environments can be effectively removed and restrained from growing when irradiation dose is 5kGy.
Description
Technical field
The present invention relates to a kind of method with algae toxin in the electron beam irradiation-induced degradation water, belong to technical field of sewage.
Background technology
Blue-green alga bloom and toxin thereof are secreted the Microcystin that the mankind are harmful to, (Microcystin) as the emphasis of body eutrophication pollution hazard.The conventional water treatment processes such as the coagulation of water treatment technique, precipitation, filtration, chlorination there is no method and effectively remove algae toxin in the water, physical method such as air supporting, membrane filtration and charcoal absorption etc. can only be transferred to another kind of medium to MCs from a kind of medium, and general chemical oxidation method such as chlorination, potassium permanganate oxidation exist formation three to cause the risk of disinfection byproduct (DBP).Although biological process does not produce secondary pollution to degrading microcystic toxins, cost is lower, and is only better at the early stage results of wawter bloom generation.Seek a kind of effective method, can degrade efficiently to Microcystin, remedy conventional water treatment process to the limitation of algae toxin degraded.
The toxicity of algae toxin is the Adda group in itself structure, is comprised of phenyl ring and conjugated double bond.Electron beam can make phenyl ring change and odorless, tasteless, is applied to the Adda group, and expectation changes the direct open loop of phenyl ring or two key, makes the algae toxin by the production intermediate product, and then is degraded into non-toxic substance.And because the MC total can be written as D-Ala-X-D-MeAsp/D-Asp-Z-Adda-D-Glu-Mdha/Dha, its special amino acid Adda structure is to express bioactive essential group, and when its structural modification or be removed, the toxicity of toxin will reduce.And the output mean power of rumbatron (comprising the low energy high current, middle energy 2-3MeV and high energy 5MeV) has reached more than the 100kW, is converted to the effective irradiation effect, and the irradiation ability much larger than domestic large-scale cobalt source can reach has reduced the radiation treatment cost.
Summary of the invention
The objective of the invention is for solve traditional water technology and technique in toxin removal process slowly, thoroughly do not reach inefficiencies, and the drawback that has simultaneously secondary pollution and " three cause " effect, the degraded that these nuclear technique means of electron beam irradiation is applied to the algae toxin is removed.Utilize electron beam to make at short notice water molecule reaction produce a large amount of OH, eaq-, H, H
3O
+Special construction Deng free radical and MC reacts, with its degraded.And by oxidation chlorophyll, by stopping metabolism, interrupt synthetic protein and frustule is dead, thus secretion and the release of termination algae toxin.In the deactivation algae, by a large amount of toxin of algae secretion, and algae toxin in the born of the same parents degraded before discharging in the degraded water body, reach in the cell and the common removal of extracellular toxin.
A kind of method with algae toxin in the electron beam degradation water of the present invention is characterized in that having following process and step: (take the toxin of microcystic aeruginosa secretion as example).
A, algae liquid is positioned over the electron beam irradiation window under the environment that opens wide, each quantity of radiant energy remains on 1.8MeV, and line is 1.0mA, and a radiation dose is 1kGy.The radiation dose that adopts in the experiment is 1kGy ~ 5kGy;
After b, radiation finish, algae liquid is refunded former culturing bottle, continue to cultivate for some time;
Get c, every day a certain amount of algae liquid, extract respectively the outer and born of the same parents' intracellular toxin of born of the same parents.Exotoxin is taked direct filtration process, and born of the same parents' intracellular toxin refilters extraction at-80 ℃ of multigelations;
D, the toxin that extracts adopt the ELISA kit measurement, and according to the change curve of toxin behind the irradiation, best radiation dose is 3kGy ~ 5kGy
Method of the present invention is its result show, the radiostimulation of 2 ~ 5kGy is remarkable on the Growth of Microcystis aeruginosa impact, and to born of the same parents' intracellular toxin, Exotoxin and total content all have obvious inhibition and removal effect.2 ~ 5kGy radiation is respectively the clearance of Exotoxin: 76.9%, 76.2%, 76.2% and 97.7%, and electron beam irradiation changes or has removed special construction in the toxin, reaches efficient degraded purpose, and along with the increase of dosage, effect is remarkable.The free radical that algae liquid absorbing radiation forms in the solution toxin of effectively having degraded.
Present method proves that the toxin that electron beam irradiation can suppress in the frustule produces and release, remedies the limitation that conventional water treatment process is removed Microcystin.Method of the present invention utilizes electron beam irradiation to remove Microcystin, and its preparation cost is cheap, and treatment effect is good, in the engineering that adopts electron beam irradiation technical finesse Source water and drinking water larger application potential is arranged.
Description of drawings
Fig. 1 be among the present invention about different radiation dose on the endotoxic impact of frustule
Fig. 2 be among the present invention about different radiation dose on the ectotoxic impact of frustule
The different radiation dose of Fig. 3 are on the impact of algae toxin total amount in the algae liquid.
Embodiment
Embodiment
Method of the present invention realizes (take the toxin of microcystic aeruginosa secretion as example) by following steps:
(1), carry out the irradiation of various dose, quantity of radiant energy remains on 1.8MeV, the setting line is 1.0mA, a radiation dose is 1kGy; The radiation dose that adopts in the experiment is respectively 1kGy, 2kGy, 3kGy, 4kGy, 5kGy.
(2), get the algae liquid of 40ml every day, extract respectively the outer and born of the same parents' intracellular toxin of born of the same parents.With algae liquid with 12000rmin
-1, 4 ℃ of centrifugal 10min, the precipitation part is stand-by; Supernatant liquor is namely measured as Exotoxin with 0.22 μ m membrane filtration, filtrate.With the frustule of collecting, use ultrapure water repetitive scrubbing 3 times, remove the toxin on frustule surface.Precipitation after the washing adds a small amount of ultrapure water, and multigelation is 3 times in-80 ℃ of cryogenic refrigerators, with 12000rmin
-1, 4 ℃ of lower centrifugal 10min, supernatant liquor is used as born of the same parents' endotoxin measurement with 0.22 μ m membrane filtration, filtrate.The toxin that extracts adopts the ELISA kit measurement.First Microcystin antibody bag is fixed on the enzyme plate, adds water sample and quantitative MC-LR-HRP conjugate during use, add chromogenic substrate at last after, chromogenic assay.
(3), the total amount of toxin add by the toxin in the outer solution of solution and born of the same parents in mensuration born of the same parents and.
(4), process by experiment, drawing the electron beam irradiation of algae-containing water is processed optimal dose is about 5Gry.
Analysis and evaluation to the process of the test among the embodiment or treating processes:
Utilize the electron beam that linear accelerator produces microcystic aeruginosa is carried out respectively 1,2,3,4 and the radiation of 5kGy, by the variation of born of the same parents' intracellular toxin and Exotoxin, analyzed that electron beam irradiation produces the frustule toxin and the impact of dispose procedure.Show that electron beam irradiation has Degradation to Microcystin.The radiostimulation of 2 ~ 5kGy is remarkable on the Growth of Microcystis aeruginosa impact, and the relevant physiological activity is affected.Generation has obvious inhibition and removal effect to born of the same parents' intracellular toxin.2 ~ 5kGy radiation is respectively the clearance of Exotoxin: 76.9%, 76.2%, 76.2% and 97.7%, the electron beam irradiation change has been described or has removed special construction in the toxin, the free radical that algae liquid absorbing radiation forms in the solution toxin of effectively having degraded reaches efficient degraded purpose.And along with the increase of dosage, effect significantly.
Claims (1)
1. method with algae toxin in the electron beam irradiation-induced degradation water is characterized in that having following process and step:
A, algae liquid is positioned over the electron beam irradiation window under the environment that opens wide, each quantity of radiant energy remains on 1.8MeV, and line is 1.0mA, and radiation dose is 1kGy ~ 5kGy;
B, radiation are refunded former culturing bottle with algae liquid after finishing, and continue to cultivate for some time;
Get c, every day a certain amount of algae liquid, extract respectively the outer and born of the same parents' intracellular toxin of born of the same parents, Exotoxin is taked direct filtration process, and born of the same parents' intracellular toxin refilters extraction at-80 ℃ of multigelations;
D, the toxin that extracts adopt the ELISA kit measurement; Determine that according to the change curve of the toxin behind the irradiation best irradiation dose is 3-5kGy.
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| CN2012103611455A CN102863047A (en) | 2012-09-26 | 2012-09-26 | Method for degradation of microcystin in water by means of electron beam irradiation |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103712848A (en) * | 2013-12-23 | 2014-04-09 | 江苏省农业科学院 | Irradiation degradation processing method of deoxynivalenol |
| CN105540732A (en) * | 2015-12-29 | 2016-05-04 | 清华大学深圳研究生院 | Method for controlling algal toxin risk without damaging algae cells |
-
2012
- 2012-09-26 CN CN2012103611455A patent/CN102863047A/en active Pending
Non-Patent Citations (2)
| Title |
|---|
| 《环境科学》 20120531 刘书宇等 "电子束辐射对铜绿微囊藻藻毒素产生和释放的抑制作用研究" 第1575-1578页 1 第33卷, 第5期 * |
| 刘书宇等: ""电子束辐射对铜绿微囊藻藻毒素产生和释放的抑制作用研究"", 《环境科学》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103712848A (en) * | 2013-12-23 | 2014-04-09 | 江苏省农业科学院 | Irradiation degradation processing method of deoxynivalenol |
| CN103712848B (en) * | 2013-12-23 | 2015-10-28 | 江苏省农业科学院 | A kind of irradiation-induced degradation facture of deoxynivalenol |
| CN105540732A (en) * | 2015-12-29 | 2016-05-04 | 清华大学深圳研究生院 | Method for controlling algal toxin risk without damaging algae cells |
| CN105540732B (en) * | 2015-12-29 | 2019-02-05 | 清华大学深圳研究生院 | A method of controlling algae toxin risk under the premise of not destroying frustule |
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Application publication date: 20130109 |