CN102858970B - 包含变体蛋白酶的组合物和方法 - Google Patents

包含变体蛋白酶的组合物和方法 Download PDF

Info

Publication number
CN102858970B
CN102858970B CN201180018494.0A CN201180018494A CN102858970B CN 102858970 B CN102858970 B CN 102858970B CN 201180018494 A CN201180018494 A CN 201180018494A CN 102858970 B CN102858970 B CN 102858970B
Authority
CN
China
Prior art keywords
amino acid
composition
cleaning
variant
protease
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201180018494.0A
Other languages
English (en)
Chinese (zh)
Other versions
CN102858970A (zh
Inventor
R·R·博特
L·G·卡斯康-佩雷拉
D·A·埃斯特尔
F·格德盖布
A·J·普洛斯
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Danisco USA Inc
Original Assignee
Danisco USA Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco USA Inc filed Critical Danisco USA Inc
Publication of CN102858970A publication Critical patent/CN102858970A/zh
Application granted granted Critical
Publication of CN102858970B publication Critical patent/CN102858970B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201180018494.0A 2010-04-15 2011-04-12 包含变体蛋白酶的组合物和方法 Active CN102858970B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US32453410P 2010-04-15 2010-04-15
US61/324,534 2010-04-15
PCT/US2011/032050 WO2011130222A2 (en) 2010-04-15 2011-04-12 Compositions and methods comprising variant proteases

Publications (2)

Publication Number Publication Date
CN102858970A CN102858970A (zh) 2013-01-02
CN102858970B true CN102858970B (zh) 2017-07-21

Family

ID=44627470

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201180018494.0A Active CN102858970B (zh) 2010-04-15 2011-04-12 包含变体蛋白酶的组合物和方法

Country Status (8)

Country Link
US (1) US20110281327A1 (enExample)
EP (1) EP2558573B1 (enExample)
JP (1) JP6018044B2 (enExample)
CN (1) CN102858970B (enExample)
AR (1) AR080886A1 (enExample)
ES (1) ES2625354T3 (enExample)
PL (1) PL2558573T3 (enExample)
WO (1) WO2011130222A2 (enExample)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090060933A1 (en) * 2004-06-14 2009-03-05 Estell David A Proteases producing an altered immunogenic response and methods of making and using the same
EP2589651A3 (en) * 2008-11-11 2013-08-28 Danisco US Inc. Compositions and methods comprising serine protease variants
CN102234810A (zh) * 2010-04-20 2011-11-09 深圳富泰宏精密工业有限公司 不锈钢蚀刻后的清洗剂及其使用方法
EP3095861B1 (en) * 2010-05-06 2019-06-26 The Procter and Gamble Company Consumer products with protease variants
WO2014200658A1 (en) 2013-06-13 2014-12-18 Danisco Us Inc. Alpha-amylase from promicromonospora vindobonensis
WO2014200656A1 (en) 2013-06-13 2014-12-18 Danisco Us Inc. Alpha-amylase from streptomyces umbrinus
WO2014200657A1 (en) 2013-06-13 2014-12-18 Danisco Us Inc. Alpha-amylase from streptomyces xiamenensis
EP3011020A1 (en) 2013-06-17 2016-04-27 Danisco US Inc. Alpha-amylase from bacillaceae family member
CN105358684A (zh) * 2013-07-29 2016-02-24 诺维信公司 蛋白酶变体以及对其进行编码的多核苷酸
US20160186102A1 (en) 2013-10-03 2016-06-30 Danisco Us Inc. Alpha-amylases from exiguobacterium, and methods of use, thereof
WO2015050723A1 (en) 2013-10-03 2015-04-09 Danisco Us Inc. Alpha-amylases from exiguobacterium, and methods of use, thereof
MX2016006489A (es) 2013-11-20 2016-08-03 Danisco Us Inc Alfa-amilasas variantes que tienen susceptibilidad reducida a la escision por proteasas y metodos de uso.
US10626388B2 (en) 2014-07-04 2020-04-21 Novozymes A/S Subtilase variants and polynucleotides encoding same
CN119709709A (zh) 2014-07-04 2025-03-28 诺维信公司 枯草杆菌酶变体以及编码它们的多核苷酸
CA2991141C (en) 2015-07-06 2022-07-19 Ecolab Usa Inc. Stain removal through novel oxidizer and chelant combination
CN108291179B (zh) 2015-11-25 2020-07-07 荷兰联合利华有限公司 液体洗涤剂组合物
WO2017173190A2 (en) 2016-04-01 2017-10-05 Danisco Us Inc. Alpha-amylases, compositions & methods
WO2017173324A2 (en) 2016-04-01 2017-10-05 Danisco Us Inc. Alpha-amylases, compositions & methods
CN109715791B (zh) * 2016-05-03 2023-07-14 丹尼斯科美国公司 蛋白酶变体及其用途
EP4525615A2 (en) 2022-05-14 2025-03-26 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
WO2024050503A1 (en) 2022-09-02 2024-03-07 Danisco Us Inc. Novel promoter and 5'-untranslated region mutations enhancing protein production in gram-positive cells
CN117947006A (zh) * 2022-10-27 2024-04-30 青岛蔚蓝生物集团有限公司 一种比活力提高的碱性蛋白酶突变体
WO2025034713A2 (en) 2023-08-09 2025-02-13 Danisco Us Inc. Compositions and methods for enhanced protein production in gram‑positive bacterial cells

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102209777A (zh) * 2008-11-11 2011-10-05 丹尼斯科美国公司 包含枯草蛋白酶变体的组合物和方法
CN102209778A (zh) * 2008-11-11 2011-10-05 丹尼斯科美国公司 包含丝氨酸蛋白酶变体的组合物和方法
CN102414321A (zh) * 2009-04-24 2012-04-11 丹尼斯科美国公司 具有修饰的原区的蛋白酶
CN102652175A (zh) * 2009-12-09 2012-08-29 宝洁公司 织物和家居护理产品
CN102762222A (zh) * 2009-12-09 2012-10-31 丹尼斯科美国公司 包含蛋白酶变体的组合物和方法
CN103122345A (zh) * 2008-11-11 2013-05-29 丹尼斯科美国公司 包含一种或多种可组合的突变的芽孢杆菌属枯草杆菌蛋白酶

Family Cites Families (104)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1296839A (enExample) 1969-05-29 1972-11-22
GB1372034A (en) 1970-12-31 1974-10-30 Unilever Ltd Detergent compositions
GB2048606B (en) 1979-02-28 1983-03-16 Barr & Stroud Ltd Optical scanning system
US4302544A (en) 1979-10-15 1981-11-24 University Of Rochester Asporogenous mutant of B. subtilis for use as host component of HV1 system
DK187280A (da) 1980-04-30 1981-10-31 Novo Industri As Ruhedsreducerende middel til et fuldvaskemiddel fuldvaskemiddel og fuldvaskemetode
GR76237B (enExample) 1981-08-08 1984-08-04 Procter & Gamble
US4450235A (en) 1982-04-21 1984-05-22 Cpc International Inc. Asporogenic mutant of bacillus subtilis useful as a host in a host-vector system
US4561998A (en) 1982-05-24 1985-12-31 The Procter & Gamble Company Near-neutral pH detergents containing anionic surfactant, cosurfactant and fatty acid
US4550862A (en) 1982-11-17 1985-11-05 The Procter & Gamble Company Liquid product pouring and measuring package with self draining feature
US4597898A (en) 1982-12-23 1986-07-01 The Proctor & Gamble Company Detergent compositions containing ethoxylated amines having clay soil removal/anti-redeposition properties
US4760025A (en) 1984-05-29 1988-07-26 Genencor, Inc. Modified enzymes and methods for making same
US4515707A (en) 1983-06-27 1985-05-07 The Chemithon Corporation Intermediate product for use in producing a detergent bar and method for producing same
IE58014B1 (en) 1983-07-06 1993-06-16 Gist Brocades Nv Molecular cloning and expression in industrial microorganism species
US4515705A (en) 1983-11-14 1985-05-07 The Procter & Gamble Company Compositions containing odor purified proteolytic enzymes and perfumes
US4537706A (en) 1984-05-14 1985-08-27 The Procter & Gamble Company Liquid detergents containing boric acid to stabilize enzymes
US5972682A (en) 1984-05-29 1999-10-26 Genencor International, Inc. Enzymatically active modified subtilisins
US5264366A (en) 1984-05-29 1993-11-23 Genencor, Inc. Protease deficient bacillus
US5763257A (en) 1984-05-29 1998-06-09 Genencor International, Inc. Modified subtilisins having amino acid alterations
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
DK154572C (da) 1985-08-07 1989-04-24 Novo Industri As Enzymatisk detergentadditiv, detergent og fremgangsmaade til vask af tekstiler
EP0218272B1 (en) 1985-08-09 1992-03-18 Gist-Brocades N.V. Novel lipolytic enzymes and their use in detergent compositions
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
EP0258068B1 (en) 1986-08-29 1994-08-31 Novo Nordisk A/S Enzymatic detergent additive
GB8629837D0 (en) 1986-12-13 1987-01-21 Interox Chemicals Ltd Bleach activation
US4765916A (en) 1987-03-24 1988-08-23 The Clorox Company Polymer film composition for rinse release of wash additives
US4972017A (en) 1987-03-24 1990-11-20 The Clorox Company Rinse soluble polymer film composition for wash additives
DE3851875T2 (de) 1987-05-29 1995-04-13 Genencor Int Cutinase haltige reinigungsmittelzusammensetzungen.
JPS6444992A (en) 1987-08-13 1989-02-17 Matsushita Electric Works Ltd Discharged lamp lighting circuit
DE3854249T2 (de) 1987-08-28 1996-02-29 Novonordisk As Rekombinante Humicola-Lipase und Verfahren zur Herstellung von rekombinanten Humicola-Lipasen.
EP0394352B1 (en) 1988-01-07 1992-03-11 Novo Nordisk A/S Enzymatic detergent
JP3079276B2 (ja) 1988-02-28 2000-08-21 天野製薬株式会社 組換え体ｄｎａ、それを含むシュードモナス属菌及びそれを用いたリパーゼの製造法
US4977252A (en) 1988-03-11 1990-12-11 National Starch And Chemical Investment Holding Corporation Modified starch emulsifier characterized by shelf stability
US4968451A (en) 1988-08-26 1990-11-06 The Procter & Gamble Company Soil release agents having allyl-derived sulfonated end caps
WO1990009446A1 (en) 1989-02-17 1990-08-23 Plant Genetic Systems N.V. Cutinase
KR100236540B1 (ko) 1990-04-14 2000-01-15 레클로우크스 라우에르 알카리성 바실러스-리파제, 이를 코-딩하는 dna 서열 및 리파제를 생산하는 바실러스 균주
US5354559A (en) 1990-05-29 1994-10-11 Grain Processing Corporation Encapsulation with starch hydrolyzate acid esters
ES2174820T3 (es) 1991-01-16 2002-11-16 Procter & Gamble Composiciones detergentes compactas con celulasa de alta actividad.
GB9108136D0 (en) 1991-04-17 1991-06-05 Unilever Plc Concentrated detergent powder compositions
US5340735A (en) 1991-05-29 1994-08-23 Cognis, Inc. Bacillus lentus alkaline protease variants with increased stability
KR100303620B1 (ko) 1992-12-01 2001-11-22 피아 스타르 효소반응의증강법
CA2154157C (en) 1993-01-18 1999-08-03 Fiona Susan Macbeath Machine dishwashing detergent compositions
EP0697036B1 (de) 1993-05-08 1999-07-28 Henkel Kommanditgesellschaft auf Aktien Silberkorrosionsschutzmittel ii
ES2112542T3 (es) 1993-05-08 1998-04-01 Henkel Kgaa Agente protector contra la corrosion de la plata i.
DK77393D0 (da) 1993-06-29 1993-06-29 Novo Nordisk As Aktivering af enzymer
US5698504A (en) 1993-07-01 1997-12-16 The Procter & Gamble Company Machine dishwashing composition containing oxygen bleach and paraffin oil and benzotriazole compound silver tarnishing inhibitors
US5486303A (en) 1993-08-27 1996-01-23 The Procter & Gamble Company Process for making high density detergent agglomerates using an anhydrous powder additive
HU219851B (hu) * 1993-10-14 2001-08-28 The Procter And Gamble Company Proteáz tartalmú tisztítókészítmények
DE4342680A1 (de) 1993-12-15 1995-06-22 Pfeiffer Erich Gmbh & Co Kg Austragvorrichtung für Medien
US5861271A (en) 1993-12-17 1999-01-19 Fowler; Timothy Cellulase enzymes and systems for their expressions
US5691295A (en) 1995-01-17 1997-11-25 Cognis Gesellschaft Fuer Biotechnologie Mbh Detergent compositions
ES2364776T3 (es) 1994-02-24 2011-09-14 HENKEL AG & CO. KGAA Enzimas mejoradas y detergentes que las contienen.
DK0701605T3 (da) 1994-02-24 2008-07-28 Henkel Ag & Co Kgaa Forbedrede enzymer og detergenter indeholdende disse
US5686014A (en) 1994-04-07 1997-11-11 The Procter & Gamble Company Bleach compositions comprising manganese-containing bleach catalysts
US6599730B1 (en) * 1994-05-02 2003-07-29 Procter & Gamble Company Subtilisin 309 variants having decreased adsorption and increased hydrolysis
PE6995A1 (es) 1994-05-25 1995-03-20 Procter & Gamble Composicion que comprende un polimero de polialquilenoamina etoxilado propoxilado como agente de separacion de sucio
PT766727E (pt) 1994-06-17 2002-11-29 Genencor Int Composicoes de limpeza contendo enzimas degradantes de paredes de celulas de plantas e seu uso em metodos de limpeza
GB2294268A (en) 1994-07-07 1996-04-24 Procter & Gamble Bleaching composition for dishwasher use
US5879584A (en) 1994-09-10 1999-03-09 The Procter & Gamble Company Process for manufacturing aqueous compositions comprising peracids
US5516448A (en) 1994-09-20 1996-05-14 The Procter & Gamble Company Process for making a high density detergent composition which includes selected recycle streams for improved agglomerate
US5691297A (en) 1994-09-20 1997-11-25 The Procter & Gamble Company Process for making a high density detergent composition by controlling agglomeration within a dispersion index
US5489392A (en) 1994-09-20 1996-02-06 The Procter & Gamble Company Process for making a high density detergent composition in a single mixer/densifier with selected recycle streams for improved agglomerate properties
ES2145315T3 (es) 1994-12-09 2000-07-01 Procter & Gamble Composicion para lavavajillas automaticos que contiene particulas de peroxidos de diacilo.
US5534179A (en) 1995-02-03 1996-07-09 Procter & Gamble Detergent compositions comprising multiperacid-forming bleach activators
US5574005A (en) 1995-03-07 1996-11-12 The Procter & Gamble Company Process for producing detergent agglomerates from high active surfactant pastes having non-linear viscoelastic properties
US5569645A (en) 1995-04-24 1996-10-29 The Procter & Gamble Company Low dosage detergent composition containing optimum proportions of agglomerates and spray dried granules for improved flow properties
BR9609284A (pt) 1995-06-16 1999-05-11 Procter & Gamble Composições para lavar louça em máquina automática compreendendo catalisadores de cobalto
US5597936A (en) 1995-06-16 1997-01-28 The Procter & Gamble Company Method for manufacturing cobalt catalysts
US5565422A (en) 1995-06-23 1996-10-15 The Procter & Gamble Company Process for preparing a free-flowing particulate detergent composition having improved solubility
DE19530816A1 (de) 1995-08-23 1997-02-27 Cognis Bio Umwelt Verwendung von mutierter Subtilisin-Protease in kosmetischen Produkten
US5576282A (en) 1995-09-11 1996-11-19 The Procter & Gamble Company Color-safe bleach boosters, compositions and laundry methods employing same
BR9610507A (pt) 1995-09-18 1999-05-04 Procter & Gamble Sistemas de liberação
MA24137A1 (fr) 1996-04-16 1997-12-31 Procter & Gamble Fabrication d'agents de surface ramifies .
US5929022A (en) 1996-08-01 1999-07-27 The Procter & Gamble Company Detergent compositions containing amine and specially selected perfumes
CN1263759C (zh) 1997-03-07 2006-07-12 宝洁公司 制备交联桥大环化合物的改进方法
CN1262632C (zh) 1997-03-07 2006-07-05 普罗格特-甘布尔公司 含有金属漂白催化剂和漂白活化剂和/或有机过羧酸的漂白组合物
GB2327947A (en) 1997-08-02 1999-02-10 Procter & Gamble Detergent tablet
US6376445B1 (en) 1997-08-14 2002-04-23 Procter & Gamble Company Detergent compositions comprising a mannanase and a protease
AR016969A1 (es) 1997-10-23 2001-08-01 Procter & Gamble VARIANTE DE PROTEASA, ADN, VECTOR DE EXPRESIoN, MICROORGANISMO HUESPED, COMPOSICIoN DE LIMPIEZA, ALIMENTO PARA ANIMALES Y COMPOSICIoN PARA TRATAR UN TEXTIL
US5935826A (en) 1997-10-31 1999-08-10 National Starch And Chemical Investment Holding Corporation Glucoamylase converted starch derivatives and their use as emulsifying and encapsulating agents
US6287839B1 (en) 1997-11-19 2001-09-11 Genencor International, Inc. Cellulase producing actinomycetes, cellulase produced therefrom and method of producing same
EP1032655B1 (en) 1997-11-21 2005-06-29 Novozymes A/S Protease variants and compositions
CA2313950A1 (en) 1997-12-24 1999-07-08 James T. Kellis, Jr. An improved method of assaying for a preferred enzyme and /or preferred detergent composition
CN101024826B (zh) 1998-06-10 2014-09-03 诺沃奇梅兹有限公司 新的甘露聚糖酶
US6376450B1 (en) 1998-10-23 2002-04-23 Chanchal Kumar Ghosh Cleaning compositions containing multiply-substituted protease variants
US6294514B1 (en) 1998-11-24 2001-09-25 The Procter & Gamble Company Process for preparing mono-long chain amine oxide surfactants with low nitrite, nitrosamine and low residual peroxide
CA2348893A1 (en) 1998-11-30 2000-06-08 The Procter & Gamble Company Process for preparing cross-bridged tetraaza macrocycles
EP1309677B2 (en) 2000-08-11 2012-04-11 Genencor International, Inc. Bacillus transformation, transformants and mutant libraries
US6440991B1 (en) 2000-10-02 2002-08-27 Wyeth Ethers of 7-desmethlrapamycin
US6582914B1 (en) 2000-10-26 2003-06-24 Genencor International, Inc. Method for generating a library of oligonucleotides comprising a controlled distribution of mutations
DK1373296T3 (da) * 2001-03-23 2012-01-09 Procter & Gamble Proteiner, der frembringer et ændret immunogent respons, og fremgangsmåder til fremstilling og anvendelse deraf
GB0114847D0 (en) 2001-06-18 2001-08-08 Unilever Plc Water soluble package and liquid contents thereof
ATE382687T1 (de) 2003-01-16 2008-01-15 Genencor Int Verfahren zur stellengerichteten mutagenese und gezielten randomisierung
DE60319347T2 (de) 2003-05-23 2009-02-19 The Procter & Gamble Company, Cincinnati Waschmittelzusammensetzung zum Gebrauch in einer Textilwasch- oder Geschirrspülmaschine
DK1680507T3 (da) 2003-11-06 2011-10-10 Danisco Us Inc Bakterieekspression af proteaseinhibitorer og varianter deraf
PT1689859E (pt) 2003-12-03 2011-06-01 Danisco Us Inc Per-hidrolase
ES2529815T3 (es) 2005-10-12 2015-02-25 The Procter & Gamble Company Uso y producción de metaloproteasa neutra estable en el almacenamiento
US20080004201A1 (en) 2006-06-05 2008-01-03 Jean-Pol Boutique Enzyme stabilizer
KR20090031906A (ko) 2006-07-18 2009-03-30 다니스코 유에스 인크. 광범위한 온도에 걸쳐 활성인 프로테아제 변이형
US8779112B2 (en) * 2007-03-12 2014-07-15 Danisco Us Inc. Modified protease propeptides
WO2008153935A2 (en) * 2007-06-06 2008-12-18 Danisco Us, Inc., Genencor Division Methods for improving protein properties
EP2100947A1 (en) 2008-03-14 2009-09-16 The Procter and Gamble Company Automatic dishwashing detergent composition
US20120067373A1 (en) * 2010-04-15 2012-03-22 Philip Frank Souter Automatic Dishwashing Detergent Composition

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102209777A (zh) * 2008-11-11 2011-10-05 丹尼斯科美国公司 包含枯草蛋白酶变体的组合物和方法
CN102209778A (zh) * 2008-11-11 2011-10-05 丹尼斯科美国公司 包含丝氨酸蛋白酶变体的组合物和方法
CN103122345A (zh) * 2008-11-11 2013-05-29 丹尼斯科美国公司 包含一种或多种可组合的突变的芽孢杆菌属枯草杆菌蛋白酶
CN102414321A (zh) * 2009-04-24 2012-04-11 丹尼斯科美国公司 具有修饰的原区的蛋白酶
CN102652175A (zh) * 2009-12-09 2012-08-29 宝洁公司 织物和家居护理产品
CN102762222A (zh) * 2009-12-09 2012-10-31 丹尼斯科美国公司 包含蛋白酶变体的组合物和方法

Also Published As

Publication number Publication date
PL2558573T3 (pl) 2017-08-31
AR080886A1 (es) 2012-05-16
CN102858970A (zh) 2013-01-02
ES2625354T3 (es) 2017-07-19
WO2011130222A3 (en) 2012-01-05
EP2558573B1 (en) 2017-02-22
US20110281327A1 (en) 2011-11-17
EP2558573A2 (en) 2013-02-20
JP2013526853A (ja) 2013-06-27
JP6018044B2 (ja) 2016-11-02
WO2011130222A2 (en) 2011-10-20

Similar Documents

Publication Publication Date Title
CN102858970B (zh) 包含变体蛋白酶的组合物和方法
US20250034542A1 (en) Protease variants and uses thereof
US20240191220A1 (en) Compositions and methods comprising serine protease variants
CN102762222B (zh) 包含蛋白酶变体的组合物和方法
CN103764823B (zh) 包含丝氨酸蛋白酶变体的组合物和方法
CN106065381B (zh) 包含丝氨酸蛋白酶变体的组合物和方法
CN102209777B (zh) 包含枯草蛋白酶变体的组合物和方法

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant