CN102851208B - High-precision cell statistic counting and analyzing device - Google Patents
High-precision cell statistic counting and analyzing device Download PDFInfo
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Abstract
A high-precision cell statistic counting and analyzing device comprises a shooting module which provides an appropriate light source for an image acquisition system, the image acquisition system which performs focusing processing of a cell image with an adjusted light source, and performs acquisition and storage, an image analysis module which analyzes the acquired cell image, calculates a gray scale histogram and a defocusing degree of the image to obtain a brightness adjusting parameter and a defocusing coefficient, feedbacks the brightness adjusting parameter and the defocusing coefficient to the shooting module and the image acquisition system for optimization, and analyzes the optimized image by an adaptive cell detection method to obtain identification results such as cell individual morphology, tissue morphology and the like, a statistic analysis module which analyzes the identification results, and statistically calculates a cell dimension histogram, a circle-ratio histogram, and an aggregation form histogram, and a data storage module which stores related results of the statistic analysis module in an excel table form and stores the related data into a database. The device of the invention is high in identification precision, and has wide application prospects in the cell detection counting field.
Description
Technical field
The present invention relates to a kind of Cell counts counting and analytical equipment, particularly relate to a kind of adaptive high-precision Cell counts counting and analytical equipment.
Background technology
Cytometric Analysis is the detection means that the biologic medical industries such as bio-pharmaceuticals are commonly used, along with the develop rapidly of biotechnology, more and more higher to the accuracy requirement of cell detection counting, for the cell digital picture obtained by microscope equipment, need to identify fast and accurately the number of cell, size and tissue morphology.And current recognition methods mainly uses binarization segmentation and morphologic relating operation to realize, accuracy of identification is not high, becomes a large obstacle of restriction cell detection counting application.
Summary of the invention
The present invention is directed to the demand and the deficiencies in the prior art, provide a kind of adaptive Cell counts counting and analytical equipment.By regulating microscopic imaging device, obtain cell microscopic sheet clearly, and a series of image manipulation is carried out to picture, get recognition result, result is added up, analyze the interested cell individual form of user and tissue morphology, comprise the number of cell, size, state, combination form etc.
For achieving the above object, the invention provides a kind of High-precision cell statistic counting and analytical equipment, comprising: photo module, image capturing system, image analysis module, statistical analysis module and data storage module, wherein,
Photo module with thinking the light source that image capturing system provides suitable, and regulates light source, supplements, make the uniform gray level of image to carry out light source to cell image;
Image capturing system is connected with photo module, in order to carry out focusing process to the cell image regulating light source, and carries out collection storage to the cell image adjusting focal length;
Image analysis module is connected with photo module and image capturing system respectively, in order to analyze the cell image collected, the grey level histogram of computed image and degree out of focus, respectively brightness regulation parameter and coefficient feedback out of focus are optimized to photo module and image capturing system after obtaining brightness regulation parameter and coefficient out of focus, and by adaptive cell detection method, the recognition results such as cell individual form and tissue morphology are analyzed to the image after optimizing;
Statistical analysis module is connected with image analysis module, in order to analyze recognition result, counts the size histogram of cell, circle rate histogram and polymeric species histogram;
Data storage module is connected with statistical analysis module, in order to preserve the correlated results of statistical analysis module by excel tabulated form and to preserve related data in database.
According to the High-precision cell statistic counting described in present pre-ferred embodiments and analytical equipment, the grey value profile of above-mentioned cell image is between 50 ~ 200.
High-precision cell statistic counting of the present invention regulates photo module and image capturing system with analytical equipment by picture recognition module, and then supplement light source to whole device by camera system, by image capturing system, cell section is gathered, and be control focusing with reference to the focal length of adjustable lens to take mark objects state, finally obtain optimum, cell microscopic image clearly.Picture recognition module also can carry out the self-adapting estimation of prosthetic interference, obtains the observed values such as the number of cell, size, state, combination form.Statistical analysis module generates histogram to analyze correlation, and final result is stored in excel form and relevant Database Lists.Compared with other recognition methods, present invention saves a large amount of workloads and identify difficulty, accuracy and the consistence of identification are guaranteed, and accuracy of identification is high.And, the present invention can analyze the interested cell individual form of user and tissue morphology, the i.e. number, size, state, combination form etc. of cell, is conducive to the testing data that follow-up testing crew analysis is relevant, extensive in cell detection counted fields application prospect.
Accompanying drawing explanation
Fig. 1 is the structure principle chart of a kind of High-precision cell statistic counting of the present invention and analytical equipment;
Fig. 2 is cell microscopic sheet to be identified in the embodiment of the present invention;
Fig. 3 is the cell analysis picture obtained after picture recognition module analysis optimization in the embodiment of the present invention;
Fig. 4 is the related data histogram that the Cell counts obtained through statistical analysis module in the embodiment of the present invention is analyzed.
Embodiment
Below in conjunction with accompanying drawing, embodiments of the invention are elaborated: the present embodiment is implemented under premised on technical solution of the present invention, give detailed embodiment and concrete operating process, but protection scope of the present invention is not limited to following embodiment.
The present embodiment realizes with analysis based on the statistical counting of vegetable cell displaing micro picture, and input picture is the microphotograph image of cell.
Refer to Fig. 1, a kind of High-precision cell statistic counting and analytical equipment, comprising: photo module 10, image capturing system 20, image analysis module 30, statistical analysis module 40 and data storage module 50, wherein,
Photo module 10 with thinking the light source that image capturing system 20 provides suitable, and regulates light source, supplements, make the uniform gray level of image to carry out light source to cell image.
The specific requirement that light source regulates is make the intensity profile of image evenly for good, and the gray-scale value overwhelming majority is distributed between 50 ~ 200, can use collected by camera program to regulate, and observes grey level histogram distribution.
Image capturing system 20 is connected with photo module 10, in order to carry out focusing process to the cell image regulating light source, and carries out collection storage to the cell image adjusting focal length.
Focal length can to take mark objects state for reference, can not out of focus and defocusing, and the focal length by adjustable lens controls, and exports picture for shown in Fig. 2.
Image analysis module 30 is connected with photo module 10 and image capturing system 20 respectively, in order to analyze the cell image collected, the grey level histogram of computed image and degree out of focus, respectively brightness regulation parameter and coefficient feedback out of focus are optimized to photo module and image capturing system after obtaining brightness regulation parameter and coefficient out of focus, and by adaptive cell detection method, the recognition results such as cell individual form and tissue morphology are analyzed to the image after optimizing.
By the digital picture that image analysis module 30 analysis collects, first the grey level histogram of computed image, judge the Luminance Distribution of image, show that brightness regulation parameter is to photo module 10, regulate brightness, then the degree out of focus of computed image, show that coefficient feedback out of focus is optimized to image capturing system 20 pairs of acquisition systems, by adaptive cell detection method, the interested cell individual form of user and tissue morphology are analyzed to the image after optimizing, comprises the number of cell, size, state, combination form etc.Concrete recognition result as shown in Figure 3;
Statistical analysis module 40 is connected with image analysis module 30, in order to analyze recognition result, counts the size histogram of cell, circle rate histogram and polymeric species histogram.Be illustrated in figure 4 size histogram;
Data storage module 50 is connected with statistical analysis module 40, in order to preserve the correlated results of statistical analysis module 40 by excel tabulated form and to preserve related data in database.
Below introduce application High-precision cell statistic of the present invention and count the detailed process of carrying out Cell counts analysis of accounts with analytical equipment.
Apply High-precision cell statistic of the present invention counting to carry out Cell counts analysis of accounts comprise the following steps with analytical equipment:
Step 1, provides suitable light source to acquisition system by photo module, and concrete requirement is to make the intensity profile of image even, and the gray-scale value overwhelming majority is distributed between 50 ~ 200, can use collected by camera program to regulate, and observes grey level histogram distribution.
Step 2, carry out collection by image capturing system to the cell image adjusting focal length and store, focal length can take mark objects state for reference, can not out of focus with defocus, controlled by the focal length of adjustable lens.
Step 3, pass through image analysis module, analyze the digital picture collected, first the grey level histogram of computed image, judges the Luminance Distribution of image, show that brightness regulation parameter is to photo module, regulate brightness, then the degree out of focus of computed image, show that coefficient feedback out of focus is optimized acquisition system to image capturing system, analyzes cell individual form and tissue morphology to the image after optimizing by adaptive cell detection method.
Step 4, the recognition result obtained by statistical analysis module analytical procedure three, counts the size histogram of cell, circle rate histogram and polymeric species histogram.
Step 5, preserves the correlated results of above-mentioned statistical analysis module by data storage module with excel tabulated form and preserves related data in database.
Above-mentioned steps 3 obtains the good picture of quality by the coefficient out of focus of brightness regulation parameter and image to acquisition system, the self-adapting estimation disturbed by prosthetic detects cell individual form and tissue morphology, counted the size histogram of cell by the analysis module shown in step 4, circle rate histogram and polymeric species histogram are stored in excel form and relevant Database Lists.
High-precision cell statistic counting of the present invention regulates photo module and image capturing system with analytical equipment by picture recognition module, and then supplement light source to whole device by camera system, by image capturing system, cell section is gathered, and be control focusing with reference to the focal length of adjustable lens to take mark objects state, finally obtain optimum, cell microscopic image clearly.Picture recognition module also can carry out the self-adapting estimation of prosthetic interference, obtains the observed values such as the number of cell, size, state, combination form.Statistical analysis module generates histogram to analyze correlation, and final result is stored in excel form and relevant Database Lists.Compared with other recognition methods, present invention saves a large amount of workloads and identify difficulty, accuracy and the consistence of identification are guaranteed, and accuracy of identification is high.And, the present invention can analyze the interested cell individual form of user and tissue morphology, the i.e. number, size, state, combination form etc. of cell, is conducive to the testing data that follow-up testing crew analysis is relevant, extensive in cell detection counted fields application prospect.
The above, it is only better embodiment of the present invention, not any pro forma restriction is done to the present invention, any content not departing from technical solution of the present invention, the any simple modification done above embodiment according to technical spirit of the present invention, equivalent variations and modification, all belong to the scope of technical solution of the present invention.
Claims (2)
1. High-precision cell statistic counting and an analytical equipment, is characterized in that, comprising: photo module, image capturing system, image analysis module, statistical analysis module and data storage module, wherein,
Described photo module with thinking that described image capturing system provides cell image, and is carried out light source to described cell image and is supplemented, and makes the uniform gray level of image;
Described image capturing system is connected with described photo module, and the photo in order to provide described photo module carries out focusing process and carry out collection to the cell image adjusting focal length storing;
Described image analysis module is connected with described photo module and image capturing system respectively, in order to analyze the cell image collected, the grey level histogram of computed image and degree out of focus, respectively brightness regulation parameter and coefficient feedback out of focus are optimized to photo module and image capturing system after obtaining brightness regulation parameter and coefficient out of focus, and by adaptive cell detection method, the recognition results such as cell individual form and tissue morphology are analyzed to the image after optimizing;
Described statistical analysis module is connected with described image analysis module, in order to analyze described recognition result, counts the size histogram of cell, circle rate histogram and polymeric species histogram;
Described data storage module is connected with described statistical analysis module, in order to by excel tabulated form preserve described in statistical analysis module correlated results and preserve related data in database.
2. High-precision cell statistic counting as claimed in claim 1 and analytical equipment, it is characterized in that, the grey value profile of described cell image is between 50 ~ 200.
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| CN107406811A (en) * | 2015-04-07 | 2017-11-28 | 奥林巴斯株式会社 | Cell resolver and cell analytic method |
| CN112161910A (en) * | 2020-09-19 | 2021-01-01 | 湖南伊鸿健康科技有限公司 | Blood cell counting method |
| CN113361395B (en) * | 2021-06-04 | 2023-01-17 | 中山大学 | AI face-changing video detection method based on multitask learning model |
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| CN101050416A (en) * | 2007-02-02 | 2007-10-10 | 杭州浙大优创科技有限公司 | Automatic counting instrument for body cell of milk / bacteria through direct microscopic method |
| CN101407759A (en) * | 2008-11-28 | 2009-04-15 | 北京理工大学 | Blood cell analyzer and analysis method |
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