CN102851208A - High-precision cell statistic counting and analyzing device - Google Patents
High-precision cell statistic counting and analyzing device Download PDFInfo
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- CN102851208A CN102851208A CN2012100625085A CN201210062508A CN102851208A CN 102851208 A CN102851208 A CN 102851208A CN 2012100625085 A CN2012100625085 A CN 2012100625085A CN 201210062508 A CN201210062508 A CN 201210062508A CN 102851208 A CN102851208 A CN 102851208A
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Abstract
A high-precision cell statistic counting and analyzing device comprises a shooting module which provides an appropriate light source for an image acquisition system, the image acquisition system which performs focusing processing of a cell image with an adjusted light source, and performs acquisition and storage, an image analysis module which analyzes the acquired cell image, calculates a gray scale histogram and a defocusing degree of the image to obtain a brightness adjusting parameter and a defocusing coefficient, feedbacks the brightness adjusting parameter and the defocusing coefficient to the shooting module and the image acquisition system for optimization, and analyzes the optimized image by an adaptive cell detection method to obtain identification results such as cell individual morphology, tissue morphology and the like, a statistic analysis module which analyzes the identification results, and statistically calculates a cell dimension histogram, a circle-ratio histogram, and an aggregation form histogram, and a data storage module which stores related results of the statistic analysis module in an excel table form and stores the related data into a database. The device of the invention is high in identification precision, and has wide application prospects in the cell detection counting field.
Description
Technical field
The present invention relates to a kind of cell statistical counting and analytical equipment, relate in particular to a kind of adaptive high-precision cell statistical counting and analytical equipment.
Background technology
The cell detection analysis is the biologic medical industry detection meanss commonly used such as bio-pharmaceuticals, develop rapidly along with biotechnology, accuracy requirement to the cell detection counting is more and more higher, for the cell digital picture that obtains by microscope equipment, need to identify fast and accurately number, size and the tissue morphology of cell.And present recognition methods mainly realizes with binarization segmentation and morphologic relating operation, and accuracy of identification is not high, becomes the large obstacle that restriction cell detection counting is used.
Summary of the invention
The present invention is directed to the demand and the deficiencies in the prior art, a kind of adaptive cell statistical counting and analytical equipment are provided.By regulating microscopic imaging device, obtain clearly cell microscopic sheet, and picture carried out a series of image manipulation, get access to recognition result, the result is added up, analyze the interested cell individual form of user and tissue morphology, comprise number, size, state, combination form of cell etc.
For achieving the above object, the invention provides a kind of high precision cell statistical counting and analytical equipment, comprising: photo module, image capturing system, image analysis module, statistical analysis module and data are preserved module, wherein,
Photo module is used and is thought that image capturing system provides suitable light source, and light source is regulated, and replenishes cell image is carried out light source, makes the uniform gray level of image;
Image capturing system is connected with photo module, in order to processing that the cell image that regulates light source is focused, and the cell image of adjusting focal length is gathered storage;
Image analysis module is connected with image capturing system with photo module respectively, the cell image that collects in order to analysis, the grey level histogram of computed image and degree out of focus, obtain behind brightness regulation parameter and the coefficient out of focus respectively the brightness regulation parameter and coefficient out of focus feeds back to photo module and image capturing system is optimized, and the image after optimizing is analyzed the recognition results such as cell individual form and tissue morphology by adaptive cell detection method;
Statistical analysis module is connected with image analysis module, in order to analyze recognition result, counts the size histogram of cell, circle rate histogram and polymeric species histogram;
Data are preserved module and are connected with statistical analysis module, in order to preserve the correlated results of statistical analysis module by the excel tabulated form and to preserve related data in database.
According to preferred embodiment of the present invention described high precision cell statistical counting and analytical equipment, the grey value profile of above-mentioned cell image is between 50～200.
High precision cell statistical counting of the present invention and analytical equipment are regulated photo module and image capturing system by picture recognition module, and then replenish light source for whole device by camera system, by image capturing system cell section is gathered, and control focusing to take the sign object state as the focal length with reference to adjustable lens, finally obtain optimum, cell microscopic image clearly.Picture recognition module also can be carried out the self-adaptation identification that prosthetic disturbs, and obtains the observed values such as number, size, state, combination form of cell.Statistical analysis module generates histogram to correlation in order to analyze, and final result is stored in excel form and the relevant Database Lists.Compare with other recognition methods, the present invention has saved a large amount of workloads and identification difficulty, and accuracy and the consistence of identification are guaranteed, and accuracy of identification is high.And, the present invention can analyze the interested cell individual form of user and tissue morphology, be the number, size, state, combination form etc. of cell, be conducive to the relevant testing data of follow-up testing crew analysis, application prospect is extensive in cell detection counting field.
Description of drawings
Fig. 1 is the structure principle chart of a kind of high precision cell statistical counting of the present invention and analytical equipment;
Fig. 2 is cell microscopic sheet to be identified in the embodiment of the invention;
Fig. 3 is the cell analysis picture through obtaining behind the picture recognition module analysis optimization in the embodiment of the invention;
Fig. 4 is the related data histogram of the cell statistical study that the process statistical analysis module obtains in the embodiment of the invention.
Embodiment
Below in conjunction with accompanying drawing embodiments of the invention are elaborated: the present embodiment is implemented under take technical solution of the present invention as prerequisite, provided detailed embodiment and concrete operating process, but protection scope of the present invention is not limited to following embodiment.
The present embodiment is based on the statistical counting and analysis realization of vegetable cell displaing micro picture, and input picture is the microphotograph image of cell.
See also Fig. 1, a kind of high precision cell statistical counting and analytical equipment comprise: photo module 10, image capturing system 20, image analysis module 30, statistical analysis module 40 and data are preserved module 50, wherein,
The specific requirement that light source is regulated is that the gray-scale value overwhelming majority is distributed between 50～200, can regulate with the collected by camera program so that the intensity profile of image evenly is good, observes grey level histogram and distributes.
Image capturing system 20 is connected with photo module 10, in order to processing that the cell image that regulates light source is focused, and the cell image of adjusting focal length is gathered storage.
Focal length can be taking the sign object state as reference, can not out of focus and defocus, can control by the focal length of adjustable lens, and the output picture is shown in Figure 2.
Analyze the digital picture that collects by image analysis module 30, the grey level histogram of computed image at first, judge the Luminance Distribution of image, draw the brightness regulation parameter to photo module 10, brightness adjusting, then the degree out of focus of computed image, drawing coefficient out of focus feeds back to 20 pairs of acquisition systems of image capturing system and is optimized, image after optimizing is analyzed the interested cell individual form of user and tissue morphology by adaptive cell detection method, comprise the number, size, state, combination form of cell etc.Concrete recognition result as shown in Figure 3;
Data are preserved module 50 and are connected with statistical analysis module 40, in order to preserve the correlated results of statistical analysis module 40 by the excel tabulated form and to preserve related data in database.
Below introduce application high precision cell statistical counting of the present invention and analytical equipment and carry out the detailed process that the cell statistical counting is analyzed.
Using high precision cell statistical counting of the present invention and analytical equipment carries out the analysis of cell statistical counting and may further comprise the steps:
Step 2 gathers storage by image capturing system to the cell image of adjusting focal length, and focal length can be taking the sign object state as reference, can not out of focus and defocus, control by the focal length of adjustable lens.
Above-mentioned steps 3 obtains preferably picture of quality by the coefficient out of focus of brightness regulation parameter and image to acquisition system, the self-adaptation recognition detection that disturbs by prosthetic goes out cell individual form and tissue morphology, count the size histogram of cell by the analysis module shown in the step 4, circle rate histogram and polymeric species histogram are stored in excel form and the relevant Database Lists.
High precision cell statistical counting of the present invention and analytical equipment are regulated photo module and image capturing system by picture recognition module, and then replenish light source for whole device by camera system, by image capturing system cell section is gathered, and control focusing to take the sign object state as the focal length with reference to adjustable lens, finally obtain optimum, cell microscopic image clearly.Picture recognition module also can be carried out the self-adaptation identification that prosthetic disturbs, and obtains the observed values such as number, size, state, combination form of cell.Statistical analysis module generates histogram to correlation in order to analyze, and final result is stored in excel form and the relevant Database Lists.Compare with other recognition methods, the present invention has saved a large amount of workloads and identification difficulty, and accuracy and the consistence of identification are guaranteed, and accuracy of identification is high.And, the present invention can analyze the interested cell individual form of user and tissue morphology, be the number, size, state, combination form etc. of cell, be conducive to the relevant testing data of follow-up testing crew analysis, application prospect is extensive in cell detection counting field.
The above, it only is better embodiment of the present invention, be not that the present invention is done any pro forma restriction, any content that does not break away from technical solution of the present invention, according to any simple modification, equivalent variations and the modification that technical spirit of the present invention is done above embodiment, all belong to the scope of technical solution of the present invention.
Claims (2)
1. a high precision cell statistical counting and analytical equipment is characterized in that, comprising: photo module, image capturing system, image analysis module, statistical analysis module and data are preserved module, wherein,
Described photo module is with thinking that described image capturing system provides cell image, and described cell image carried out light source replenish, and makes the uniform gray level of image;
Described image capturing system is connected with described photo module, focuses in order to the photo that described photo module is provided and processes and the cell image of adjusting focal length is gathered storage;
Described image analysis module is connected with image capturing system with described photo module respectively, the cell image that collects in order to analysis, the grey level histogram of computed image and degree out of focus, obtain behind brightness regulation parameter and the coefficient out of focus respectively the brightness regulation parameter and coefficient out of focus feeds back to photo module and image capturing system is optimized, and the image after optimizing is analyzed the recognition results such as cell individual form and tissue morphology by adaptive cell detection method;
Described statistical analysis module is connected with described image analysis module, in order to analyze described recognition result, counts the size histogram of cell, circle rate histogram and polymeric species histogram;
Described data are preserved module and are connected with described statistical analysis module, in order to the correlated results of preserving described statistical analysis module by the excel tabulated form and preservation related data in database.
2. high precision cell statistical counting as claimed in claim 1 and analytical equipment is characterized in that, the grey value profile of described cell image is between 50～200.
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| CN107406811A (en) * | 2015-04-07 | 2017-11-28 | 奥林巴斯株式会社 | Cell resolver and cell analytic method |
| CN112161910A (en) * | 2020-09-19 | 2021-01-01 | 湖南伊鸿健康科技有限公司 | Blood cell counting method |
| CN113361395A (en) * | 2021-06-04 | 2021-09-07 | 中山大学 | AI face-changing video detection method based on multitask learning model |
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| CN113361395A (en) * | 2021-06-04 | 2021-09-07 | 中山大学 | AI face-changing video detection method based on multitask learning model |
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