CN102847509B - Adsorbent for mycotoxin in feed and preparation method thereof - Google Patents
Adsorbent for mycotoxin in feed and preparation method thereof Download PDFInfo
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- CN102847509B CN102847509B CN201210126269.5A CN201210126269A CN102847509B CN 102847509 B CN102847509 B CN 102847509B CN 201210126269 A CN201210126269 A CN 201210126269A CN 102847509 B CN102847509 B CN 102847509B
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Abstract
The invention discloses an adsorbent for mycotoxin in a feed and a preparation method thereof, aiming at providing an aluminosilicate adsorbent for adsorbing mycotoxin in a mouldy animal feed. The adsorbent is prepared by using medical stone powder as a raw material, immersing the medical stone powder in a certain ratio of antibacterial material quaternary ammonium salt-myristyl trimethyl ammonium bromide, stirring, washing, filtering, drying, and grinding into powder. According to the invention, the processed modified medical stone powder has an increased internal surface area and an enhanced adsorption capacity; by adding the modified medical stone powder in the feed, the animal immunity function and production performance can be raised, and the toxic action of mycotoxin in the feed to animals can be alleviated.
Description
Technical field
This invention relates to feed safety field, is specifically related to a kind of preparation method of mycotoxins in feed adsorbent.
Background technology
Claim according to FAO (Food and Agriculture Organization of the United Nation), approximately there is in the world 25% cereal to be subject to some extent the pollution of mycotoxin, there are grain and the feed of 5%-7% to lose feeding value because go mouldy, the livestock and poultry mycotoxin poisonings such as the livestock and poultry death that caused by mycotoxin, productivity decline, reproductive capability obstacle occur repeatedly, and economic loss is very serious.Feed has been become very important problem in feed industry and animal husbandry production by mould and mycotoxin contamination.Meanwhile, mycotoxin also can be residual in animal products, for mankind's health care belt carrys out great potential safety hazard.Finding that suitable effective method controls it is the task of top priority of animal husbandry development, and in feed, adding adsorbent is a kind of more convenient and economic method.Processing at present the topmost mode of feed of going mouldy is to use mold toxin sorbent.
Mineral medical stone (medical stone) is a kind of weathering or the shallow one-tenth of the half weathering middle acidic rock to super shallow one-tenth, contain the multiple trace element useful to human body, principal character is poor calcium, magnesium, rich potassium, sodium meta-alkalescence, wherein SiO2, Al2O3 both account for altogether more than 70%.At present, in the world medical stone research field is related to the aspects such as medical treatment, health food, daily-use chemical industry, environmental protection.In animal husbandry and fishery aquaculture, medical stone is as the mineral feed of livestock and poultry fishes and shrimps or the carrier of animal feed additive premix, respond well.The adsorptivity of medical stone shows to have cellular and spongelike structure, and specific area is quite large, and the higher molecule of polarity is had to stronger adsorption capacity, can adsorb the ammonia harmful to animal health, hydrogen sulfide, organochlorine, cyanide, fusel wet goods material; In addition, the main chemical compositions of medical stone is SiO2, can form with [SiO4]
4-for basis, tetrahedron top oxygen and adjacent tetrahedron be the space network structure of key altogether, with K
+, Na
+, Ca
2+, Mg
2+by ionic bond combination.
The suction-operated of medical stone can be used for purifying water and processing industrial wastewater, can adsorb harmful bacteria (as Escherichia coli etc.), harmful element (as lead, mercury, cadmium etc.) and toxic gas (as ammonia, sulfur dioxide etc.) in animal alimentary canal, thereby improve environment in stomach and intestine, strengthen stomach and intestine function, be conducive to digesting and assimilating and animal body health of nutriment, promote growth of animal, improve efficiency of feed utilization.Medical stone has gap structure and ion stripping property is widely used in the research of purification of water quality aspect, and the research of mycotoxin in feed mould is common in to montmorillonite, the bentonite etc. of Aluminosilicates.
Summary of the invention
The present invention discloses a kind of preparation method of mycotoxins in feed adsorbent, the method has improved the effective rate of utilization of medical stone, make suction-operated more obvious, can alleviate Mycotoxins in Feed and pollute the toxic action to piglet, can guarantee feed quality and livestock birds health.
The following technology of employing of the present invention:
A preparation method for mycotoxins in feed adsorbent, method is as follows:
(1) natural medical stone is ground into 100 object particles;
(2) by weight with volume ratio (mg: ml) maifanite powder: A solution=30: 100,
Described A solution is that to contain mass fraction be 10% TTAB, the ethanolic solution that volumetric concentration is 50%, 60 ℃ of waters bath with thermostatic control vibration 1h, and the centrifugal supernatant that inclines,
(3) again with 60 ℃ of water bath with thermostatic control vibration 1h, the centrifugal supernatant that inclines;
(4) take out medical stone, by weight with volume ratio (mg: the ml) distilled water immersion of 30: 100,60 ℃ of water bath with thermostatic control vibration 1h;
(5) after suction filtration, be placed in 105 ℃ of baking ovens and dry 3h, cooling rear grounds travel is broken into 100 order particles, for subsequent use;
The present invention also has following feature: the medical stone adding after modification by mass fraction 1% in feed.
Method of the present invention has improved the internal surface area of medical stone absorption and exchange, has guaranteed the effective rate of utilization of medical stone, makes suction-operated more obvious, has strengthened absorption property, can alleviate Mycotoxins in Feed and pollute the toxic action to piglet.
Accompanying drawing explanation
Fig. 1 is the affect schematic diagram of unmodified medical stone Different adding amount on adsorption rate;
Fig. 2 is the affect schematic diagram of modification medical stone Different adding amount on adsorption rate;
Fig. 3 is the affect schematic diagram of pH value on modification medical stone adsorption rate;
Fig. 4 is the affect schematic diagram of temperature on modification medical stone adsorption rate;
Fig. 5 is the affect schematic diagram of time on modification medical stone adsorption rate.
The specific embodiment
Below by embodiment, technical scheme of the present invention is described further.
(1) modified test: take 30g natural medical stone, be crushed to 100 order particles, joined in 100mlA solution, described A solution is that to contain mass fraction be 10% TTAB, the ethanolic solution that volumetric concentration is 50%; 60 ℃ of water bath with thermostatic control vibration 1h, repeat this operation 1 time after the centrifugal supernatant that inclines; After filtration, with the distilled water immersion of 100ml, 60 ℃ of water bath with thermostatic control vibration 1h; Again filter, be placed in 105 ℃ of baking ovens and dry 3h, grind after cooling for subsequent use.
(2) in vitro test:
A. the impact of unmodified medical stone Different adding amount on adsorption rate
As shown in Figure 1, adsorbent addition is respectively 10,25,50,75,100mg, mycotoxin in each pipe--zearalenone (ZON) 5000ng, with each centrifuge tube of PBS solution constant volume of pH=8.0 to 10ml, after 25 ℃ of constant temperature oscillation absorption 1h, the centrifugal 10min of 4000r/min, HPLC method is measured ZON content in supernatant.
B. the impact of modification medical stone Different adding amount on adsorption rate
As shown in Figure 2, adsorbent adds respectively 3,5,7,10,13mg, and ZON is 5000ng, with each centrifuge tube of PBS solution constant volume of pH=8.0 to 10ml, after 25 ℃ of constant temperature oscillation absorption 1h, the centrifugal 10min of 4000r/min, HPLC method is measured ZON content in supernatant.
The impact of C.PH value on modification medical stone adsorption rate
As shown in Figure 3, adsorbent 10mg, ZON is 5000ng, with pH=2.0,4.0,6.0, each centrifuge tube of PBS solution constant volume of 8.0 to 10ml, after 25 ℃ of constant temperature oscillations absorption 1h, the centrifugal 10min of 4000r/min, HPLC method is measured ZON content in supernatant.
D. the impact of temperature on modification medical stone adsorption rate
As shown in Figure 4, adsorbent 10mg, ZON is 5000ng,, adsorbs after 1h at 25 ℃, 37 ℃, 60 ℃ constant temperature oscillations respectively to 10ml with each centrifuge tube of PBS solution constant volume of pH=8.0, the centrifugal 10min of 4000r/min, HPLC method is measured ZON content in supernatant.
E. the impact of time on modification medical stone adsorption rate
As shown in Figure 5, adsorbent 10mg, ZON is 5000ng, with each centrifuge tube of PBS solution constant volume of pH=8.0 to 10ml, vibrate respectively after absorption 10min, 30min, 60in, 90min, 120min at 25 ℃ of constant temperature, the centrifugal 10min of 4000r/min, HPLC method is measured ZON content in supernatant.
(3) in vivo studies
In the daily ration (corn-soybean meal) that is 1mg/kg containing zearalenol (ZON) piglet (about 12kg left and right), add the modification medical stone adsorbent of mass fraction 1%, compared with control group, piglet growth performance increases; ZON intoxicating phenomenon is alleviated to some extent, reduced the toxic action of ZON to piglets organ; Improve blood parameters, improved immunity function.
| Control group | Processed group | |
| Average daily gain (g/d) | 620 | 651 |
| Average daily ingestion amount (g/d) | 723 | 736 |
| Feedstuff-meat ratio | 1.16 | 1.13 |
| Liver index | 25.9 | 25.2 |
Claims (3)
1. a preparation method for mycotoxins in feed adsorbent, is characterized in that, method is as follows:
(1) natural medical stone is ground into 100 object particles;
(2) by weight with volume ratio (mg: ml) maifanite powder: A solution=3: 10,
Described A solution is that to contain mass fraction be 10% TTAB, the ethanolic solution that volumetric concentration is 50%, 60 ℃ of waters bath with thermostatic control vibration 1h, and the centrifugal supernatant that inclines,
(3) again with 60 ℃ of water bath with thermostatic control vibration 1h, the centrifugal supernatant that inclines;
(4) take out medical stone, by weight with volume ratio (mg: the ml) distilled water immersion of 30: 100,60 ℃ of water bath with thermostatic control vibration 1h;
(5) after suction filtration, be placed in 105 ℃ of baking ovens and dry 3h, cooling rear grounds travel is broken into 100 order particles, for subsequent use.
2. a kind of mycotoxins in feed adsorbent that the preparation method of a kind of mycotoxins in feed adsorbent according to claim 1 makes.
3. a kind of mycotoxins in feed adsorbent according to claim 2, is characterized in that: in feed, add adsorbent by mass fraction 1%.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103521173A (en) * | 2013-10-12 | 2014-01-22 | 东北农业大学 | Adsorbing agent capable of adsorbing zearalenone in feed and preparation method thereof |
| MX338482B (en) * | 2013-11-25 | 2016-04-19 | Nutek S A De C V | Mycotoxin absorbent and the use thereof in balanced food for animals. |
| CN105211635B (en) * | 2015-10-26 | 2018-05-18 | 金华傲农生物科技有限公司 | A kind of feed addictive for releasing mycotoxin and preparation method and application |
| CN106179194A (en) * | 2016-07-06 | 2016-12-07 | 浙江丰虹新材料股份有限公司 | A kind of modified clay and its preparation method and application |
| CN107646885B (en) * | 2017-08-07 | 2020-05-22 | 吴杨 | High-efficiency sterilization deodorant and preparation method thereof |
| CN107362784A (en) * | 2017-09-15 | 2017-11-21 | 江苏科力特环保科技有限公司 | A kind of stain remover |
| CN107997297A (en) * | 2017-12-11 | 2018-05-08 | 宾度投资股份有限公司 | One kind promoting blood circulation, antibacterial, hidroschesis Traditional Chinese medicinal insole |
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Non-Patent Citations (4)
| Title |
|---|
| 南阳麦饭石性能及改性研究;王斌;《中国优秀硕士学位论文全文数据库 工程科技Ⅰ辑》;20120315;25-27 * |
| 张惠芳等.麦饭石对霉菌毒素AME AOH的吸附清除实验.《河南肿瘤学杂志》.2001,第14卷(第1期),21-22. |
| 王斌.南阳麦饭石性能及改性研究.《中国优秀硕士学位论文全文数据库 工程科技Ⅰ辑》.2012,25-27. |
| 麦饭石对霉菌毒素AME AOH的吸附清除实验;张惠芳等;《河南肿瘤学杂志》;20010228;第14卷(第1期);21-22 * |
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