CN102830206B - Method for detecting swill-cooked dirty oil by using biology tracer technique - Google Patents
Method for detecting swill-cooked dirty oil by using biology tracer technique Download PDFInfo
- Publication number
- CN102830206B CN102830206B CN201210262743.7A CN201210262743A CN102830206B CN 102830206 B CN102830206 B CN 102830206B CN 201210262743 A CN201210262743 A CN 201210262743A CN 102830206 B CN102830206 B CN 102830206B
- Authority
- CN
- China
- Prior art keywords
- oil
- antioxidant
- sample
- waste oil
- tracer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The invention discloses a method for detecting swill-cooked dirty oil by using a biology tracer technique. An antioxidant capable of carrying out thermal decomposition is added in fresh edible oil in a scope of food additives, the antioxidant and thermal decomposition matters of the antioxidant serve as tracers, the oil can be detected to be the swill-cooked dirty oil if any thermal decomposition matter of the antioxidant is detected. With the adoption of the method, a swill-cooked dirty oil sample which comprises a sample of the fresh edible oil doped with the swill-cooked dirty oil can be effectively identified, the tracers generated by the thermal decomposition are various and not easy to separate and remove, so that accordingly circulation of the swill-cooked dirty oil on the market is avoided.
Description
Technical field
The invention belongs to biological trace method and detect waste oil field, relate in particular to a kind of method of utilizing biological tracer technique to detect waste oil.
Background technology
Waste oil is as a kind of poor oil, all severe overweights such as its heavy metal, toxin, peroxide value, and contain the class chemical impurities such as washing agent, serious harm the mankind healthy.A lot of illegal retailers, in order reaping staggering profits, after the simple processing such as washing, distillation, decolouring is processed, to treat as waste oil edible oil or mix in fresh food oil and sell.Although national correlation department has been put into effect some measures (on March 18th, 2010, office of State Food and Drug Administration issues " about being strictly on guard against that " waste oil " flows into food and beverage sevice link emergency notice " (food medicine prison is done food [2010] No. 25)), but owing to detecting the hysteresis of waste oil technology, cannot tackle the problem at its root.At present, the main methods such as physical and chemical index analysis, heavy metal detection, fatty acid compositional analysis, Determination of Cholesterol Content, electrical conductivity method, thin-layered chromatography, fluorescence method of using are carried out the detection of waste oil both at home and abroad, in application, there is certain advantage, can detect some characteristic indexs of waste oil, but be difficult to differentiate to being mixed with the sample of a small amount of waste oil (≤15%) in fresh food oil; Moreover for the managerial personnel of food hygiene, detect length consuming time, step complexity, equipment investment is large, testing cost is high, be difficult for a series of defects such as execute-in-place is the urgent realistic problem solving.So applying new waste oil detection method has great importance to the management of waste oil.
Biological tracer technique be add from the outside with biosome in element or the tracer of the completely common operation of material, in order to follow the trail of certain element or the operation of something or a kind of method of variation in biosome.Tracer be for observing, research and measure the behavior of something in assignment procedure or a kind of label that character adds.Given this technology has advantages of process tracking, thus be widely used in fields such as medical science, agricultural and animal husbandry, as diagnose the illness, study biological mechanics etc.Because illegal retailer has carried out various simple low-cost processes to waste oil, cause great difficulty to detection.
Summary of the invention
The object of the invention is to propose the method for the biological tracer technique detection of a kind of utilization quickly and efficiently that utilizes biological tracer technique to carry out waste oil.
The present invention adopts following technical scheme to achieve these goals:
A kind of method of utilizing biological tracer technique to detect waste oil, it is characterized in that: in the scope of food additives, add the antioxidant that thermal decomposition can occur in fresh food oil, taking described antioxidant and pyrolysate thereof as tracer, if there is the pyrolysate of any one antioxidant to be detected, can show that this oil is for waste oil.
A kind of described method of utilizing biological tracer technique to detect waste oil, is characterized in that: the described antioxidant that thermal decomposition can occur refers to the antioxidant that thermal decomposition can occur at 150-250 DEG C.
Described antioxidant is the one in ascorbyl palmitate, ascorbic acid lactate.
A kind of described method of utilizing biological tracer technique to detect waste oil, is characterized in that:
Comprise the following steps:
(1) add the antioxidant that thermal decomposition can occur in fresh food oil, it is fully dissolved;
(2) using direct as detected sample the heat treated fresh food oil that contains antioxidant, or be incorporated in fresh food oil as detected sample in≤15% ratio;
(3) pyrolysate of antioxidant in the detected sample of detecting step (2), if there is any one pyrolysate to be detected, can show that this oil is for waste oil.
Principle of the present invention is: the present invention has introduced a kind of method of utilizing biological tracer technique to detect waste oil, in the scope of food additives, add the antioxidant that thermal decomposition can occur in fresh food oil, antioxidant and pyrolysate thereof are tracer, get in market interpolation after circulation the edible oil of antioxidant detect, if there is any one antioxidant pyrolysate to be detected, can show that this oil was heated one or many, can be defined as waste oil, if the tracer detecting is antioxidant itself, show that this oil is not waste oil.This method is from source, edible oil to be controlled, applicability is strong, easy to operate, and after thermal decomposition, tracer kind is many, be difficult for removing, also for industrial and commercial administration department, health law enforcement agency and food superintendent office provide convenience to the management of waste oil, fundamentally stop the circulation of waste oil on market simultaneously, safeguarded consumer's interests.
Beneficial effect of the present invention: utilize biological tracer technique to there is process control.The invention provides a kind of method of utilizing biological tracer technique to detect waste oil; Compared with former method, the method can effectively be differentiated waste oil sample, comprises the sample that is mixed with waste oil in fresh food oil, and the tracer kind that thermal decomposition produces is many, not easily separated removing, thus stop the circulation of waste oil on market.
Brief description of the drawings
Fig. 1 utilizes biological trace method to detect the process flow diagram of waste oil in the present invention.
embodiment,
As shown in Figure 1, select two kinds of antioxidants as embodiment, the present invention is described in further detail.
example 1
Choose ascorbyl palmitate as antioxidant, determine whether waste oil by the detection to tracer, concrete implementation step is as follows:
(1) preparation of detected sample
Sample 1: add≤0.2 g ascorbyl palmitate in the fresh food oil of 1kg.Be placed at the temperature of 100～250 DEG C and heat 1～500 time, each 5～20 min, then cooling, be the detected sample 1 of simulation waste oil.
Sample 2: sample 1 is mixed in fresh food oil in≤15% ratio, mix, be the sample 2 of simulation waste oil.
(2) detect the tracer in sample
Tracer has ascorbyl palmitate itself, ascorbyl palmitate is under different heating degrees, to produce some pyrolysate tracers, have respectively, ascorbic acid, palmitic acid, hydroascorbic acid, diketogulonic acid etc., according to the kind of tracer select accordingly, accurately, fast, rational detection method.
(3) criterion
If there is any one antioxidant pyrolysate to be detected, can show that this oil was heated one or many, be waste oil, if the tracer detecting is antioxidants ascorbic acid palmitate itself, show that this oil is not waste oil.
example 2
Choose ascorbic acid lactate as antioxidant.
(1) preparation of detected sample
Sample 1: add≤0.2 g ascorbic acid lactate in the fresh food oil of 1kg.Be placed at the temperature of 100～250 DEG C and heat 1～500 time, each 5～20 min, then cooling, be the detected sample 1 of simulation waste oil.
Sample 2: sample 1 is mixed in fresh food oil in≤15% ratio, mix, be the sample 2 of simulation waste oil.
(2) detect the tracer in sample
Tracer has: ascorbic acid lactate, ascorbic acid, lactic acid, hydroascorbic acid, diketogulonic acid etc.
(3) criterion
Be the ascorbic acid lactate pyrolysates such as ascorbic acid, lactic acid, hydroascorbic acid, diketogulonic acid if detect tracer, can show that this oil was heated one or many, for waste oil, if the tracer detecting is ascorbic acid lactate, show that this oil is not waste oil.
example 3
Choose the antioxidant of ascorbyl palmitate as fresh food oil, utilize its pyrolysate for biological tracer, determine whether waste oil by the detection to tracer, concrete implementation step is as follows:
(1) preparation of detected sample
Sample 1: adding 0.1 g ascorbyl palmitate in the fresh food oil of 1kg is sample 1.
Sample 2: sample 1 is placed at the temperature of 170 DEG C and heats 200 times, each 10 min, then cooling, be modeled to waste oil (because have a lot of illegal retailers when fried on market, its fried number of times almost has 1-500 time more than), be detected sample 2.
Sample 3: sample 2 is mixed in fresh food oil in 10% ratio, mix, be sample 3.
(2) detect the tracer in sample
Tracer has ascorbyl palmitate itself, ascorbyl palmitate is under different heating degrees, to produce some pyrolysate tracers, have respectively, ascorbic acid, palmitic acid, hydroascorbic acid, diketogulonic acid etc., according to the kind of tracer select accordingly, accurately, fast, rational detection method.
(3) criterion
If there is any one antioxidant pyrolysate to be detected, can show that this oil was heated one or many, be waste oil, if the tracer detecting is antioxidants ascorbic acid palmitate itself, show that this oil is not waste oil.
The tracer that sample 1 detects is that antioxidants ascorbic acid palmitate, sample 2, sample 3 have antioxidant pyrolysate tracer to be detected.Known this method is effective for detection of waste oil.
example 4
Choose the antioxidant of ascorbic acid lactate as fresh food oil, utilize its pyrolysate for biological tracer, determine whether waste oil by the detection to tracer, concrete implementation step is as follows:
(1) preparation of detected sample
Sample 1: adding 0.1 ascorbic acid lactate in the edible oil precursor of 1kg is sample 1.
Sample 2: sample 1 is placed at the temperature of 170 DEG C and heats 100 times, each 10 min, then cooling, be modeled to waste oil (because have a lot of illegal retailers when fried on market, its fried number of times almost has 1-500 time more than), be detected sample 2.
Sample 3: sample 2 is mixed in fresh food oil in 10% ratio, mix, be sample 3.
(2) detect the tracer in sample
Tracer has: ascorbic acid lactate, ascorbic acid, lactic acid, hydroascorbic acid, diketogulonic acid etc.
(3) criterion
Be the ascorbic acid lactate pyrolysates such as ascorbic acid, lactic acid, hydroascorbic acid, diketogulonic acid if detect tracer, can show that this oil was heated one or many, for waste oil, if the tracer detecting is ascorbic acid lactate, show that this oil is not waste oil.
The tracer that sample 1 detects is that ascorbic acid lactate, sample 2, sample 3 have ascorbic acid lactate pyrolysate tracer to be detected.Known this method is effective for detection of waste oil.
Claims (4)
1. a method of utilizing biological tracer technique to detect waste oil, it is characterized in that: in the scope of food additives, add the antioxidant that thermal decomposition can occur in fresh food oil, taking described antioxidant and pyrolysate thereof as tracer, if there is any one antioxidant pyrolysate to be detected, can show that this oil is for waste oil.
2. a kind of method of utilizing biological tracer technique to detect waste oil according to claim 1, is characterized in that: the described antioxidant that thermal decomposition can occur refers to the antioxidant that thermal decomposition can occur at 150-250 DEG C.
3. a kind of method of utilizing biological tracer technique to detect waste oil according to claim 1, is characterized in that: described antioxidant is the one in ascorbyl palmitate, ascorbic acid lactate.
4. a kind of method of utilizing biological tracer technique to detect waste oil according to claim 1, is characterized in that:
Comprise the following steps:
(1) add the antioxidant that thermal decomposition can occur in fresh food oil, it is fully dissolved;
(2) be incorporated in fresh food oil as detected sample using the heat treated fresh food oil that contains antioxidant as detected sample, or in≤15% ratio;
(3) pyrolysate of antioxidant in the detected sample of detecting step (2), if there is any one pyrolysate to be detected, can show that this oil is for waste oil.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210262743.7A CN102830206B (en) | 2012-07-26 | 2012-07-26 | Method for detecting swill-cooked dirty oil by using biology tracer technique |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210262743.7A CN102830206B (en) | 2012-07-26 | 2012-07-26 | Method for detecting swill-cooked dirty oil by using biology tracer technique |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102830206A CN102830206A (en) | 2012-12-19 |
| CN102830206B true CN102830206B (en) | 2014-10-29 |
Family
ID=47333414
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210262743.7A Active CN102830206B (en) | 2012-07-26 | 2012-07-26 | Method for detecting swill-cooked dirty oil by using biology tracer technique |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102830206B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104483342B (en) * | 2014-12-13 | 2017-12-26 | 广西科技大学 | A kind of method of qualitative detection hogwash fat |
| CN106290647B (en) * | 2016-08-30 | 2018-09-25 | 成都新润油脂有限责任公司 | A kind of edible oil quality detecting system and detection method |
| CN108709940A (en) * | 2016-08-30 | 2018-10-26 | 黄燕云 | A kind of edible oil of quality safety |
| CN109425674B (en) * | 2017-08-29 | 2021-11-19 | 成都市食品药品检验研究院 | Tracer agent for detecting waste edible oil |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101294939A (en) * | 2006-12-29 | 2008-10-29 | 天津科技大学 | Method for measuring content of polar compound in grease |
| CN101387612A (en) * | 2008-07-23 | 2009-03-18 | 昆明理工大学 | Method for detecting waste oil in qualified edible oil |
| CN101808531A (en) * | 2007-09-28 | 2010-08-18 | 约翰森·马瑟公开有限公司 | Tagging method |
| CN102539639A (en) * | 2011-12-27 | 2012-07-04 | 何联泉 | Method for marking and finding illegal cooking oil |
-
2012
- 2012-07-26 CN CN201210262743.7A patent/CN102830206B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101294939A (en) * | 2006-12-29 | 2008-10-29 | 天津科技大学 | Method for measuring content of polar compound in grease |
| CN101808531A (en) * | 2007-09-28 | 2010-08-18 | 约翰森·马瑟公开有限公司 | Tagging method |
| CN101387612A (en) * | 2008-07-23 | 2009-03-18 | 昆明理工大学 | Method for detecting waste oil in qualified edible oil |
| CN102539639A (en) * | 2011-12-27 | 2012-07-04 | 何联泉 | Method for marking and finding illegal cooking oil |
Non-Patent Citations (10)
| Title |
|---|
| 《"地沟油"鉴别技术研究展望》;曹文明等;《中国油脂》;20120520;第37卷(第5期);第2-4页 * |
| 《L-抗坏血酸棕榈酸酯在煎炸油中应用研究》;何松等;《现代食品科技》;20100930;第26卷(第9期);全文 * |
| 《精制食用油及其加热产物的化学成分分析》;王云翔等;《铁道医学》;19990331;第27卷(第6期);全文 * |
| 《食品抗氧化剂及其进展》;凌关庭;《粮食与油脂》;20010131(第1期);全文 * |
| 《食用油脂抗氧化剂及其安全性研究进展》;李书国等;《粮食与油脂》;20060531(第5期);全文 * |
| 何松等.《L-抗坏血酸棕榈酸酯在煎炸油中应用研究》.《现代食品科技》.2010,第26卷(第9期),全文. |
| 凌关庭.《食品抗氧化剂及其进展》.《粮食与油脂》.2001,(第1期),全文. |
| 曹文明等.《"地沟油"鉴别技术研究展望》.《中国油脂》.2012,第37卷(第5期),第2-4页. |
| 李书国等.《食用油脂抗氧化剂及其安全性研究进展》.《粮食与油脂》.2006,(第5期),全文. |
| 王云翔等.《精制食用油及其加热产物的化学成分分析》.《铁道医学》.1999,第27卷(第6期),全文. |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102830206A (en) | 2012-12-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Batz et al. | Attributing illness to food | |
| Mikhail et al. | An outbreak of Shiga toxin-producing Escherichia coli O157: H7 associated with contaminated salad leaves: epidemiological, genomic and food trace back investigations | |
| CN102830206B (en) | Method for detecting swill-cooked dirty oil by using biology tracer technique | |
| Pacholewicz et al. | Propidium monoazide does not fully inhibit the detection of dead Campylobacter on broiler chicken carcasses by qPCR | |
| Peng et al. | In vitro catabolism of quercetin by human fecal bacteria and the antioxidant capacity of its catabolites | |
| Das et al. | Effect of freeze drying and oven drying on antioxidant properties of fresh wheatgrass | |
| Naseri et al. | Lipid changes during long-term storage of canned sprat | |
| He et al. | Differentiation between fresh and frozen–thawed meat using rapid evaporative ionization mass spectrometry: the case of beef muscle | |
| CN104946790A (en) | PCR method for tracking source identification of origin components of eight kinds of animals | |
| Yi et al. | Prediction in the dynamics and spoilage of Shewanella putrefaciens in Bigeye Tuna (Thunnus obesus) by gas sensors stored at different refrigeration temperatures | |
| CN102778545A (en) | Method for comprehensively and quickly screening multiparameters of illegal cooking oil | |
| Silva et al. | Aroma profile and consumer liking of salted and dried chicken meat: Effects of desalting and cooking methods | |
| CN102590405A (en) | Identification method for illegal cooking oil | |
| CN104946743A (en) | Fluorescent quantitative PCR method for identifying tuna species and types of highly processed products of tuna species | |
| CN103525908A (en) | Method for rapidly detecting chicken, duck and pig blood components in blood jelly | |
| Kaufmann et al. | Analysis of polyphosphates in fish and shrimps tissues by two different ion chromatography methods: Implications on false-negative and-positive findings | |
| He et al. | Inhibiting effects of ginger and rosemary on the formation of heterocyclic amines, polycyclic aromatic hydrocarbons, and trans fatty acids in fried pork balls | |
| CN104007188B (en) | A kind of gas chromatograph detects the method for waste oil | |
| Upadhaya et al. | Effects of ionised or chelated water-soluble mineral mixture supplementation on growth performance, nutrient digestibility, blood characteristics, meat quality and intestinal microbiota in broilers | |
| Kamili et al. | Determination of zinc in camel skin using laser-induced breakdown spectroscopy | |
| CN106568902A (en) | Surgical smoke biological toxicity evaluation method | |
| CN102121044A (en) | System and method for rapidly detecting pesticide residues in fruits and vegetables on site | |
| Gumus et al. | Propolis extract reduces heterocyclic aromatic amine formation in chicken thigh meat | |
| Zhang et al. | The Research Field of Meat Preservation: A Scientometric and Visualization Analysis Based on the Web of Science | |
| Indramali et al. | THE FACTORS AFFECTING FOOD SECURITY IN URBAN AREAS |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |