CN106568902A - Surgical smoke biological toxicity evaluation method - Google Patents

Surgical smoke biological toxicity evaluation method Download PDF

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Publication number
CN106568902A
CN106568902A CN201610983587.1A CN201610983587A CN106568902A CN 106568902 A CN106568902 A CN 106568902A CN 201610983587 A CN201610983587 A CN 201610983587A CN 106568902 A CN106568902 A CN 106568902A
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toxicity
operation smoke
bio
smoke
toxicity evaluation
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陆钢
佘少桦
谢绮彤
苏靖仪
钟子浩
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Jinan University
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Jinan University
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/0004Gaseous mixtures, e.g. polluted air

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  • Life Sciences & Earth Sciences (AREA)
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  • General Health & Medical Sciences (AREA)
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Abstract

The invention discloses a surgical smoke biological toxicity evaluation method. The method comprises the following steps: A, evaluating the in-vitro biological toxicity of luminescent bacteria; B, evaluating the in-vivo biological toxicity of ApoE gene-deficient mice exposed to surgical smoke; and C, evaluating the genetic toxicity of surgical smoke through micronucleus test. The method of the invention has the advantages of quickness and efficiency, accuracy and quantification, realness and reliability, multi-level evaluation, and the like, and has very high practical value and a broad market prospect. The method gets away from the embarrassing situation in which the biological toxicity of surgical smoke cannot be tested fully, and is expected to become an important way of evaluation and test for controlling surgical smoke pollution.

Description

A kind of operation smoke bio-toxicity evaluation methodology
Technical field
The present invention relates to a kind of operation smoke bio-toxicity evaluation methodology.
Background technology
With continuous development of the Minimally Invasive Surgery in modern medicine, the operation smoke produced in operation process has to health Very big harm, can cause the acute and chronic poisoning of human body, operation smoke to directly result in operating room healthcare givers and patient There are the symptoms such as headache, eye nose larynx pain inflammation.Pathogen is also possible to be propagated by operation smoke diffusion, causes operating room Healthcare givers is infected.Operation smoke can also produce genetoxic, and numerous studies prove that operation smoke has potential mutagenicity With carcinogenecity, genetoxic can be produced.But, these years since, due to the restriction and the cognition of level of condition, for operation The research of smog is few, and particularly the country does not almost have, the approach cognitive so as to reduce people.Plus due to cigarette of performing the operation Mist does not typically result in the acute and chronic poisoning with manifest symptom, thus is easily ignored by people.But operating room is cured Treatment personnel are exposed to containing including due to long-time:Imperfect combustion tissue fragment, active pathogen and virus, cancer Become the handss of the harmful substances such as cell, toxic and harmful substance, CO, acrylonitrile, formaldehyde, benzene homologues (particularly toluene), phenols In art smog, chronicity, repeatedly contact cause poisonous effect to accumulate, so should be taken seriously, and the measure that properly protects.
At present, it is of common occurrence for the chemical analysis method of single toxic compounds in operation smoke, bio-toxicity evaluation Data are also no lack of report.However, lacking for complicated component, the joint-biotoxicity of the miscellaneous operation smoke of noxious substance Method for quantitatively evaluating invention and creation, future is expected to become the ecological risk assessment of operation smoke and ecological pollution control Technical tool.
The content of the invention
To solve the shortcoming and defect part of prior art, it is an object of the invention to provide a kind of biology of operation smoke Toxicity assessment method.The method is a kind of comprehensive bio-toxicity evaluation methodology of employing in vitro and in vivo, for operation smoke pair The evaluation areas of human health damage and ecological risk, can provide for the ecological risk of operation smoke and to human health damage Technology evaluation meanses.
The object of the invention is achieved through the following technical solutions:
A kind of operation smoke bio-toxicity evaluation methodology, comprises the following steps:A, photobacteria external biological toxicity assessment; B, ApoE deficient mice bio-toxicity evaluation in vivo under operation smoke exposure, vivo biodistribution toxicity assessment result makes up body The deficiency of outer biological toxicity assessment result;C, micronucleus test evaluate operation smoke genetoxic.
It is well known that operation smoke has the features such as chemical toxicity is relatively low, bio-toxicity is relatively low, and photobacteria and other Antibacterial is compared, with sensitivity it is high the characteristics of, and response speed is fast, low cost, simple to operate, is suitable for operation smoke poison The detection of property.In the culture fluid incubation containing dissolving operation smoke solvent, photobacteria is by harmful substance in operation smoke Poison, bacterial respiratory is reduced with physiological process activity, so as to bacterial luminescence intensity changes, by determining photobacteria The bio-toxicity of luminous intensity quantitatively characterizing operation smoke.
Preferably, described photobacteria is Vibrio-qinghaiensis sp. Q67 (Vibrio qinghaiensis sp.-Q67).
The Vibrio-qinghaiensis sp. Q67 (Vibrio qinghaiensis sp.-Q67) of the present invention belongs to well known in the prior art Bacterial strain, it is all on the books in many books or periodical literature, books such as written by red legend outstanding person (red legend is outstanding, Zheng Tianling, Li Weimin. send out Photobacteria is detected with environmental toxicity【M】. Beijing:China Light Industry Press, 2009.175~225), the document report of MAMEI etc. (MAMEI, virgin China, Wang Zijian, Yang Ducai, red legend is outstanding. new luminescent bacterium (Vibrio qinghaiensis sp.-Q67) It is applied to the preliminary study of environmental sample toxotest.《ACTA Scientiae Circumstantiae》, 01 phase in 1998).Vibrio-qinghaiensis sp. Q67 is a kind of Rare fresh water photobacteria, is characterized in compared with other Marine Luminous Bacterias, need not add during culture larger amount of NaCl (up to 3%), so as to avoid the possibility thus brought from affecting.After appropriate CMC model, Vibrio-qinghaiensis sp. Q67 can send Macroscopic blue green light.And Vibrio-qinghaiensis sp. Q67 is adapted to middle isosmoticity, adaptive temperature scope close with general cell Larger (10~30 DEG C), have wider adaptive faculty (adapting to pH=5.5~9.0) to pH, so preferably using Qinghai in the present invention Vibrio Q67.
Preferably, the bio-toxicity evaluation methodology of the operation smoke specifically includes following step:
A () carries out external biological toxicity quantitative assessment to operation smoke by detecting Vibrio-qinghaiensis sp. Q67 luminous intensity, obtain Bio-toxicity evaluation quantifies testing result;
B () is exposed to median lethal concentration operation smoke with 6 monthly age ApoE deficient mices as biological subject material In, blood fat and histopathology situation are checked, compensate for operation smoke external biological toxicity method for quantitatively evaluating can not detect not The deficiency of insoluble gas, obtains the true detection by quantitative result of operation smoke bio-toxicity;
C () makes cell smear with the bone marrow of step (b) mice, basis of microscopic observation contains the thermophilic of micronucleus more and incarnadines carefully Born of the same parents, according to the micronuclear rateses of polychromatic erythrocyte, evaluate the genetoxic of operation smoke.
It is furthermore preferred that select ethanol to be GAS ABSORPTION liquid in step (a), it is dense in different pollutant from Vibrio-qinghaiensis sp. Q67 Certain hour is acted in the GAS ABSORPTION liquid of degree, index is turned to the change of its luminous intensity, it is possible to achieve be quick, efficient, accurate Quantitatively evaluating operation smoke bio-toxicity.
Ethanol can dissolve most organic exhaust gas, and the impact of its bio-toxicity to photobacteria is less, institute To be suitable as the absorbing liquid of operation smoke.
It is furthermore preferred that the inspection blood fat and histopathology situation described in step (b), specifically refers to:Check mice blood Lipid level and aorta tangent plane Lipid Plaque situation of change.
The photobacteria luminous intensity external biological toxicity method for quantitatively evaluating mentioned in step (a) is present can not be detected not The deficiency of insoluble gas toxicity, needs, by checking ApoE deficient mices blood fat and histopathology situation in (b), to enter one Step is modified the result in external biological toxicity assessment method, reduces true bio-toxicity evaluation result.
For the operation smoke of complicated component, Luminous bacteria can not detect the gas insoluble in ethanol, such as CO, CO2, because This, it is comprehensive to adopt representative mammal, ApoE deficient mices to receive as vivo biodistribution toxicity assessment method Examination is biological, more sensitive to the endocrine disruption toxicity of operation smoke, can be with accurate simulation operation smoke contaminated air after, human body Bio-toxicity effect in intake, metabolic process, more closing to reality pollution condition and poisonous effect.It is simultaneously biological to contamination Medullary cell carry out micronucleus test, detect its genetoxic, acquired results can correct Luminous bacteria evaluation result not Foot so that the bio-toxicity evaluation result of operation smoke is more comprehensively and true and reliable.
Step (c) takes mouse bone marrow cells in step (b) and makes cell smear, examines under a microscope polychromatic erythrocyte, counts Calculate micronuclear rateses;Micronuclear rateses refer to the polychromatic erythrocyte number containing micronucleus, and with permillage it is represented;According to micronuclear rateses to operation smoke Genetoxic evaluated.
Compared with prior art, the present invention has advantages below and beneficial effect:
(1) high sensitivity of photobacteria, it is all very sensitive to many toxicants, more adapt to cigarette of performing the operation compared with other antibacterials The low concentration of mist, hypotoxicity.Using Vibrio-qinghaiensis sp. Q67, it is not necessary to add substantial amounts of NaCl, osmotic pressure medium, adaptive temperature and pH Scope is larger, can more accurately realize the bio-toxicity of quantitative assessment operation smoke, and operation smoke composition is solved well Complicated, external biological evaluation carrier selects difficult, it is impossible to detects the pressing problem of joint-biotoxicity, is expected to become government and ring Protect the tool method that monitoring department detects operation smoke pollution situation.
(2) present invention adopts ApoE deficient mice vivo biodistribution toxicity assessment methods, more truly simulates sky After gas is polluted by operation smoke, bio-toxicity effect of the human body in intake, metabolic process more truly reflects operation smoke The poisonous effect of actual pollution condition and human body.The biological genetoxic of contamination is detected simultaneously, can make up external biological toxicity The deficiency of evaluation methodology so that the bio-toxicity evaluation result of operation smoke is more true and reliable.
To sum up, the method for the invention have rapidly and efficiently, precise quantification, true and reliable, mutiple-stage model etc. it is many excellent Point, with very high practical value and wide market prospect, is expected to become the important evaluation detection of control operation smoke pollution Means.
Specific embodiment
With reference to embodiment, the present invention is described in further detail, but embodiments of the present invention not limited to this.
Embodiment 1
Step one:Vibrio-qinghaiensis sp. Q67 recovery bacterium solution is prepared, 10ml operation smokes are gathered in 5ml ethanol with gas flowmeter In absorbing liquid, the photobacteria suspension of 0.1mL is added to respectively the operation smoke sample of warmed-up (or cold) to 20~25 DEG C In stock solution, 1.25 times of dilution, 2.5 times of dilution, 5 times of dilution, the 1ml experiment water samples of 10 times of dilution (3 Duplicate Samples of each concentration), Accurately after 15~20min of effect, its luminous intensity is surveyed successively.Record sample liquid concentration and its luminous intensity values, and with testing tube In luminous intensity as control light intensity (the interior sample liquid for fresh air), calculate relative suppression light rate.It is with sample concentration Abscissa, relative suppression light rate are vertical coordinate mapping, and the relative corresponding sample liquid concentration in the place of light rate 50% that presses down is water sample EC50 values, that is, luminous intensity be maximum emission intensity half when exhaust gas concentration value.
Experimental result shows, operation smoke sample stock solution, 1.25 times of dilution, 2.5 times of dilution, 5 times of dilution, 10 times of dilution The relative suppression light rate of Vibrio-qinghaiensis sp. Q67 is 70%, 60%, 40%, 30%, 20% in experiment water sample, and the relative light rate that presses down is with operation Linear relationship is good between the concentration of smog, calculates EC50 for 1.22ml/ml.Drawn according to the percent grade that Bulich is proposed Minute mark is accurate, and it is micro- poison that conclusion is the bio-toxicity that operation smoke has, although will not cause injury immediately to human body, but for a long time Suction accumulation in situations in the surgery room can have certain infringement to human body.
Step 2:5 preliminary experiment groups, 10 per group 6 monthly age ApoE deficient mices (mice sources are set:And south University life science and technology institute), wherein female, male mice is each 5.The mice being grouped is taken, is put in dynamic formula toxicant exposure cabinet, prohibit water Fasting.Operation smoke concentration is 60.5ml/m in operating room3, according to design dosage and each toxicant exposure cabinet volume, calculating needs what is added The volume of operation smoke, makes each toxicant exposure cabinet operation smoke concentration determine 5,10,20,40, the 80 of concentration close to operating room respectively Times concentration.Then operation smoke is passed through in cabinet, timing 12h, and records contamination in-cabinet temperature, observation animal poisoning symptom and dead Die the indexs such as number of individuals.Median lethal concentration LC is measured according to experimental result50For 2.42 × 103ml/m3。
5 experimental grouies and 1 matched group, 10 per group 6 monthly age ApoE deficient mices, male and female half and half are set.Experiment Mice is put in dynamic formula toxicant exposure cabinet group, fasting for solids and liquids, and setting concentration of contamination is median lethal concentration LC50.During contamination, matched group Mice is also removed water and removes food, and receives fresh air.The jejunitas 12h of mice, orbital venous plexus take blood, centrifugation, separate serum, determine little Mus T-CHOL (TC), low-density lipoprotein cholesterol (LDL-C), HDL-C (HDL-C), triglyceride (TG), apolipoprotein B.Compared with matched group, experimental group TC and LDL-C are significantly raised, and HDL-C is significantly reduced, apolipoprotein B water Flat is 1.5~3 times of control group mice.At the disconnected marrow of mice after death, whole-heartedly frozen section is carried out after dirty specimen liquid nitrogen quenching, is cut Piece oil red O (ORO) dyes the speckle formational situation of the different tangent planes of observation, and experimental group plaque area is the 1.5~2 of matched group Times, comparing with external biological toxicity assessment result, external biological toxicity assessment conclusion is correct.
Meanwhile, the bone marrow smear of experimental mice is prepared, carry out Micronucleus test.Observation is used containing micronucleus Polychromatic erythrocyte.Polychromatic erythrocyte (PCE) is in dusty blue, and acellular core, mature erythrocyte (NCE) is in light salmon pink. Micronucleus great majority are in single circle, and the smooth of the edge is neat, and thermophilic color is consistent with caryoplasm, in aubergine or bluish violet.Per only moving Thing at least counts 1000 polychromatic erythrocytes.Micronuclear rateses refer to the polychromatic erythrocyte number containing micronucleus, with permillage (‰) table Show it.And calculate the ratio of PCE/NCE in 200 cells, normal PCE/NCE ratios are about 1 (normal 0.6~1.2), and real The average PCE/NCE ratios of group mice are tested less than 0.5, is represented that PCE is formed and is suppressed by thing of contaminating.
Above-described embodiment is the present invention preferably embodiment, but embodiments of the present invention not by above-described embodiment Limit, other any spirit without departing from the present invention and the change, modification, replacement made under principle, combine, simplification, Equivalent substitute mode is should be, is included within protection scope of the present invention.

Claims (5)

1. a kind of operation smoke bio-toxicity evaluation methodology, it is characterised in that comprise the following steps:A, photobacteria external biological Toxicity assessment;The bio-toxicity evaluation in vivo under operation smoke exposure of B, ApoE deficient mice;C, micronucleus test evaluate handss Art smog genetoxic.
2. a kind of operation smoke bio-toxicity evaluation methodology according to claim 1, it is characterised in that described is luminous thin Bacterium is Vibrio-qinghaiensis sp. Q67.
3. a kind of operation smoke bio-toxicity evaluation methodology according to claim 2, it is characterised in that specifically include following Step:
A () carries out external biological toxicity quantitative assessment to operation smoke by detecting Vibrio-qinghaiensis sp. Q67 luminous intensity, obtain biological Toxicity assessment quantifies testing result;
B () in being exposed to median lethal concentration operation smoke, is examined with 6 monthly age ApoE deficient mices as biological subject material Have a blood test fat and histopathology situation, compensate for operation smoke external biological toxicity method for quantitatively evaluating can not detect insoluble gas The deficiency of body, obtains the true detection by quantitative result of operation smoke bio-toxicity;
C () makes cell smear with the bone marrow of step (b) mice, basis of microscopic observation contains the polychromatic erythrocyte of micronucleus, According to the micronuclear rateses of polychromatic erythrocyte, the genetoxic of operation smoke is evaluated.
4. a kind of operation smoke bio-toxicity evaluation methodology according to claim 3, it is characterised in that choosing in step (a) It is GAS ABSORPTION liquid with ethanol, Vibrio-qinghaiensis sp. Q67 is acted on into certain hour in the GAS ABSORPTION liquid of different pollutant levels, with The change of its luminous intensity turns to index, obtains bio-toxicity evaluation and quantifies testing result.
5. a kind of operation smoke bio-toxicity evaluation methodology according to claim 3, it is characterised in that institute in step (b) The inspection blood fat stated and histopathology situation, specifically refer to:Check that lipid of mice level and aorta tangent plane Lipid Plaque become Change situation.
CN201610983587.1A 2016-11-09 2016-11-09 Surgical smoke biological toxicity evaluation method Pending CN106568902A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110133190A (en) * 2019-05-13 2019-08-16 常州大学 A kind of method of airborne particulates parameter in bio kinetic model
CN112730738A (en) * 2020-12-07 2021-04-30 中国科学院深圳先进技术研究院 Evaluation device and evaluation method for reward and addiction of atomized matter

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101543429A (en) * 2008-03-27 2009-09-30 范维林 Movable smoke inhalation control device
CN201757749U (en) * 2010-08-04 2011-03-09 中国人民解放军第三军医大学 Simulated sealed cabinet smoke contamination experiment device
CN201811951U (en) * 2010-04-28 2011-04-27 范维林 Toxicity test device for fume generated by burning materials
CN102453743A (en) * 2010-10-28 2012-05-16 华东师范大学 Method for preparing source water quality acute biotoxicity detection reagent and detection method of detection reagent
CN105548514A (en) * 2015-12-25 2016-05-04 哈尔滨工业大学 Multi-genetic terminal biological group test method for evaluating genetic toxicity of industrial wastewater reuse of water treatment plant
CN205461795U (en) * 2016-03-18 2016-08-17 广州迪佰瑞环保科技有限公司 Operation smog processing apparatus
CN105987898A (en) * 2015-03-05 2016-10-05 上海市南洋模范中学 Detection method for acute biological toxicities of pollutants on dust-haze day

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101543429A (en) * 2008-03-27 2009-09-30 范维林 Movable smoke inhalation control device
CN201811951U (en) * 2010-04-28 2011-04-27 范维林 Toxicity test device for fume generated by burning materials
CN201757749U (en) * 2010-08-04 2011-03-09 中国人民解放军第三军医大学 Simulated sealed cabinet smoke contamination experiment device
CN102453743A (en) * 2010-10-28 2012-05-16 华东师范大学 Method for preparing source water quality acute biotoxicity detection reagent and detection method of detection reagent
CN105987898A (en) * 2015-03-05 2016-10-05 上海市南洋模范中学 Detection method for acute biological toxicities of pollutants on dust-haze day
CN105548514A (en) * 2015-12-25 2016-05-04 哈尔滨工业大学 Multi-genetic terminal biological group test method for evaluating genetic toxicity of industrial wastewater reuse of water treatment plant
CN205461795U (en) * 2016-03-18 2016-08-17 广州迪佰瑞环保科技有限公司 Operation smog processing apparatus

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
曲东: "《环境监测》", 31 August 2007, 中国农业出版社 *
李义平等: "被动吸烟对小鼠致突变作用", 《西安医科大学学报》 *
王玲等: "手术烟雾危害及防护措施研究进展", 《护理学报》 *
罗建军等: "香烟烟雾对大鼠血脂和脂质过氧化的影响", 《实用预防医学》 *
金平正等: "《卷烟烟气安全性与危害防范》", 30 September 2009, 中国轻工业出版社 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110133190A (en) * 2019-05-13 2019-08-16 常州大学 A kind of method of airborne particulates parameter in bio kinetic model
CN112730738A (en) * 2020-12-07 2021-04-30 中国科学院深圳先进技术研究院 Evaluation device and evaluation method for reward and addiction of atomized matter

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