CN102816686B - PCR (polymerase chain reaction) amplification instrument and application method thereof - Google Patents
PCR (polymerase chain reaction) amplification instrument and application method thereof Download PDFInfo
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- CN102816686B CN102816686B CN201210314149.8A CN201210314149A CN102816686B CN 102816686 B CN102816686 B CN 102816686B CN 201210314149 A CN201210314149 A CN 201210314149A CN 102816686 B CN102816686 B CN 102816686B
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Abstract
The invention discloses a PCR (polymerase chain reaction) amplification instrument and an application method thereof. The application method of the disclosed PCR amplification instrument comprises the following steps: assigning reference guide data according to a preset command; enabling a pointer parameter be equal to the sequence number of the reference guide data in a guide data column, and enabling an intermediate data parameter be equal to the reference guide data; updating the pointer parameter and the intermediate data parameter; judging whether the pointer parameter is greater than a preset value; if no, returning to the cycle; if so, terminating the cycle; determining that the guide data of which the sequence number in the guide data column is equal to the pointer parameter is target guide data, enabling the value of the target guide data to be equal to the value of the intermediate data parameter, and then returning; and enabling each virtual sample parameter to be equal to guide data corresponding to the virtual sample parameter. In the technical scheme, the value of the guide data in the guide data column can be automatically generated according to the arrangement and numbers of actual samples, thereby ensuring the one-to-one correspondence relationship between virtual sample parameters and actual sample numbers, and reducing errors of the virtual sample parameters.
Description
Technical field
The present invention relates to a kind of Bioexperiment detection technique, be specifically related to a kind of pcr amplification instrument.
Background technology
Current, generally use Polymerase Chain Reaction (Polymerase chain reaction is called for short PCR) amplification instrument to carry out PCR experiment.Pcr amplification instrument comprises and adds model, adds and on model, is provided with a plurality of sample wells that hold or place sample.Utilize pcr amplification instrument to carry out PCR when experiment, add in the sample well of model and place sample, sample can be common sample, can be also standard model.Pcr amplification instrument can detect and obtain sample message to a plurality of samples.
For the ease of distinguishing, sample generally has corresponding numbering, can determine the source main body (patient or other staff) of sample by this numbering.For the ease of pcr amplification instrument, distinguish the sample message obtaining after sample analysis, the sample being placed in sample well is arranged by its number order conventionally; Meanwhile, in pcr amplification instrument, be provided with memory module, store virtual sample parameters group in described memory module, virtual sample parameters group comprises a plurality of virtual sample parameters of arranging by predetermined rule; In general, virtual sample parameters is corresponding one by one with the sample in sample well.
After sample after numbering is placed in sample well, need particular location per sample and the numbering of sample, give the occurrence of corresponding virtual sample parameters, make virtual sample parameters corresponding one by one with sample number into spectrum.Like this, at pcr amplification instrument, corresponding sample is analyzed and obtained after sample information, make sample body information corresponding with virtual sample parameters, and then export virtual sample parameters and sample body information; By the corresponding relation between virtual sample parameters and sample number into spectrum, can make to set up corresponding relation between actual sample and sample body information again.
In order to give the occurrence of respective virtual sample parameters, current pcr amplification instrument is provided with numbering input unit and modular converter conventionally.After in each sample is placed on sample well by predetermined order, staff, according to the position of actual sample, inputs sample number into spectrum in modular converter with the order of being scheduled to one by one by input unit is manual; Modular converter gives virtual sample parameters corresponding occurrence according to predetermined input sequence, makes virtual sample parameters corresponding one by one with the numbering of actual sample.
Due to the sample number of each experiment have a lot of parts (such as: 96 parts or 124 parts), not only speed is slow for manual input sample number into spectrum, wastes time and energy, and also easily occurs sample number into spectrum input error, causes number with Louing, goes here and there number and the fastening one person's story upon another person of number; This will make virtual sample parameters mistake, and virtual sample parameters is not corresponding with the numbering of actual sample.In addition, owing to need to carrying out repeatedly PCR experiment every day, will increase like this possibility of sample number into spectrum input error.
In the situation that virtual sample parameters is not corresponding with the numbering of actual sample, in experimentation, the sample message that pcr amplification instrument obtains will be corresponding with wrong actual sample numbering, and the sample message that pcr amplification instrument is exported may be not corresponding with actual sample; And then, the sample message of pcr amplification instrument analysis acquisition and the sign of main body are not inconsistent, the judgement of impact to main body physical appearance.Will cause the wrong report to patient's assay like this, affect result for the treatment of, even can cause medical tangle to produce.
Therefore, how reducing the virtual sample parameters mistake of pcr amplification instrument is the major issue that current PC R amplification instrument is used.
Summary of the invention
One object of the present invention is to provide a kind of using method of pcr amplification instrument, adopts this using method can reduce the mistake of virtual sample parameters.
Another object of the present invention is to provide a kind of pcr amplification instrument, utilizes this pcr amplification instrument also can reduce the mistake of virtual sample parameters.
In addition, in the using method of the pcr amplification instrument that the present invention also provides, described pcr amplification instrument comprises memory module and auxiliary storage module, presets at least one virtual sample parameters group in described memory module, and virtual sample parameters group comprises a plurality of virtual sample parameters; Described auxiliary storage module presets pointer parameter, intermediate data parameter, at least one pilot data row, and described pilot data row comprise a plurality of pilot data of arranging with predefined procedure; A plurality of described pilot data are corresponding one by one with a plurality of virtual sample parameters;
This using method comprises:
Step S110, determines that according to predetermined instruction predetermined pilot data is benchmark pilot data, carries out assignment to this benchmark pilot data;
Step S120, makes pointer parameter equal the sequence number of benchmark pilot data in pilot data row, and makes intermediate data parameter equal benchmark pilot data;
Step S130, makes pointer parameter increase by 1, makes intermediate data parameter increase Y;
Step S140, judges whether pointer parameter is greater than preset value, if not, enters step S150; If so, enter step S160;
Step S150, determines that the pilot data that sequence number in pilot data row equals pointer parameter is target guiding data, and makes the value of target guiding data equal the value of intermediate data parameter; Return to step S130;
Step S160, makes each virtual sample parameters equal the pilot data corresponding with this virtual sample parameters.
Optionally, before described step S110, also comprise the step S100 that initial value is preset; Step S100 comprises:
The initial value that makes pointer parameter is 1.
Optionally, step S100 also comprises: the initial value of setting intermediate data parameter.
Optionally, in step S130, make intermediate data parameter increase by 1.
Optionally, in step S110, predetermined pilot data is the pilot data of sequence number minimum in described pilot data row.
Pcr amplification instrument provided by the invention comprises memory module, presets at least one virtual sample parameters group in described memory module, and virtual sample parameters group comprises a plurality of virtual sample parameters, it is characterized in that, also comprises:
Auxiliary storage module, presets pointer parameter, intermediate data parameter, at least one pilot data row, and described pilot data row comprise a plurality of pilot data of arranging with predefined procedure; A plurality of described pilot data are corresponding one by one with a plurality of virtual sample parameters;
Initial assignment module, for determining that according to predetermined instruction predetermined pilot data is benchmark pilot data, carries out assignment to this benchmark pilot data; Make again pointer parameter equal the sequence number of benchmark pilot data in pilot data row, make intermediate data parameter equal benchmark pilot data, then produce pointer shift order;
Counting pointer locating module, for according to pointer shift order, makes pointer parameter increase by 1, makes intermediate data parameter increase Y;
Judge module, for judging whether pointer parameter is greater than preset value, if not, produces follow-up assignment directive; If so, produce conversion instruction;
Follow-up assignment module, for according to follow-up assignment directive, determines that the pilot data that sequence number in pilot data row equals pointer parameter is target guiding data, and makes the value of target guiding data equal intermediate data parameter; Then produce pointer shift order;
Described modular converter, for making each virtual sample parameters equal the pilot data corresponding with this virtual sample parameters according to conversion instruction.
Optionally, the initial value of described pointer parameter is 1.
Optionally, pcr amplification instrument also comprises input unit, and described input unit is for inputting and set the initial value of intermediate data parameter.
Optionally, described Y is 1.
Optionally, initial assignment module determines that according to predetermined instruction predetermined pilot data is in benchmark pilot data, and predetermined pilot data is the pilot data of sequence number minimum in described pilot data row.
While utilizing the using method of pcr amplification instrument provided by the invention, can first sample be placed in sample well according to sample number into spectrum, pcr amplification instrument is by arranging auxiliary storage module, can preset pointer parameter, intermediate data parameter, at least one pilot data row, described pilot data row comprise a plurality of pilot data of arranging with predefined procedure; A plurality of described pilot data are corresponding one by one with a plurality of virtual sample parameters.In this using method, first according to predetermined instruction, determine that predetermined pilot data is benchmark pilot data, carries out assignment to this benchmark pilot data; Make again pointer parameter equal the sequence number of benchmark pilot data in pilot data row, make intermediate data parameter equal benchmark pilot data; Again pointer parameter and intermediate data parameter are upgraded, according to pointer parameter and intermediate data parameter after upgrading, select corresponding pilot data to carry out assignment again, after assignment, can turn back to again and upgrade pointer parameter and middle data parameters, then select corresponding pilot data to carry out assignment according to pointer parameter and intermediate data parameter after upgrading; Like this, by repeatedly circulating and can realize the automatic assignment to pilot data row; In the pilot data assignment procedure to pilot data example, can also determine whether in pilot data example after the whole assignment of the pilot data in pre-determined range according to pointer parameter; If so, make each virtual sample parameters equal the pilot data corresponding with this virtual sample parameters.This technical scheme can be arranged and numbering according to actual sample, the value of pilot data in pilot data row is generated automatically, and the value of pilot data is arranged sequentially, and then can make the occurrence of virtual sample parameters also with the order of being scheduled to, arrange, guarantee the one-to-one relationship of virtual sample parameters and actual sample numbering, reduce the mistake of virtual sample parameters, guarantee to analyze the sample body information of acquisition and the corresponding relation of actual sample.
Pcr amplification instrument provided by the invention, also can produce corresponding technique effect, realizes the wrong object that reduces virtual sample parameters in pcr amplification instrument.
Accompanying drawing explanation
Fig. 1 is the using method schema of a kind of pcr amplification instrument of providing of the embodiment of the present invention;
Fig. 2 is that in the using method of the pcr amplification instrument that provides of the embodiment of the present invention, sample is adding the distribution plan of model.
Embodiment
Below in conjunction with accompanying drawing, technical scheme provided by the invention is described in detail, the description of this part is only exemplary and explanatory, should not be considered as the restriction to public technology content of the present invention.
Please refer to Fig. 1, this figure is the using method schema of a kind of pcr amplification instrument of providing of the embodiment of the present invention.This figure only illustrates the part steps of the using method of pcr amplification instrument, and other steps can adopt step in prior art.
In the using method of the pcr amplification instrument that the present embodiment provides, pcr amplification instrument comprises two storing devices: a storing device is memory module, and another storing device is auxiliary storage module.In memory module, preset a virtual sample parameters group; Virtual sample parameters group comprises a plurality of virtual sample parameters.Auxiliary storage module presets pointer parameter, intermediate data parameter, pilot data row; Described pilot data row comprise a plurality of pilot data of arranging with predefined procedure.And a plurality of described pilot data are corresponding one by one with a plurality of virtual sample parameters.In the present embodiment, pointer parameter initial value is 0, and intermediate data initial parameter value is 0.
Current, when using pcr amplification instrument, first the sample with predefined procedure numbering is placed in the sample well that adds model; Like this, each sample is corresponding with a virtual sample parameters respectively; Now, the concrete numerical value of virtual sample parameters is not consistent with the numbering of each position sample, and the enforcement of following steps can make the concrete numerical value of virtual sample parameters and the numbering of each position sample be consistent.
Please refer to Fig. 1, this using method comprises the following steps:
Step S110, determines that according to predetermined instruction predetermined pilot data is benchmark pilot data, carries out assignment to this benchmark pilot data.
In this step, predetermined instruction can be the steering order that operator input, and input mode can be passed through computer keyboard, mouse or other input units.In preferred embodiment, can click and form predetermined instruction by mouse.When sample starts application of sample from adding first sample well of model, predetermined pilot data can be the most front pilot data in pilot data row, i.e. the pilot data of sequence number minimum in pilot data row.Certainly, at actual sample, not when adding first sample well application of sample of model, predetermined pilot data can be the pilot data of appropriate location in pilot data row.Such as: please refer to Fig. 2, this figure is that in the using method of the pcr amplification instrument that provides of the embodiment of the present invention, sample is adding the distribution plan of model.1141,1142,1143,1144 if actual sample 201 starts to arrange from adding the first sample well of model 200, sample number into spectrum is: ....Can select first pilot data is benchmark pilot data, and it is 1141 that this benchmark pilot data is carried out to assignment.
Step S120, makes pointer parameter equal the sequence number of benchmark pilot data in pilot data row, and makes intermediate data parameter equal benchmark pilot data.Can realize like this pointer parameter location, make intermediate data parameter consistent with the benchmark pilot data of assignment.Making pointer parameter is 1, and intermediate data parameter is 1141.
Step S130, makes pointer parameter increase by 1, makes intermediate data parameter increase Y.Can realize like this renewal of the intermediate data parameter of pointer parameter.Y can determine according to the regularity of distribution of actual sample numbering, in actual sample number order, adds 1 while increasing progressively, and the value of Y can be 1.Making pointer parameter is 2, and intermediate data parameter is 1142.
Step S140, judges whether pointer parameter is greater than preset value, if not, enters step S150; If so, enter step S160.The object of this step is to judge that specifically whether upgrading of pilot data is complete; Not upgrading when complete, enter step S150, other pilot data are carried out to assignment renewal; When renewal is complete, enter step S160.The preset value per sample scope of actual quantity and sample place sample well determines, larger at sample actual quantity, this preset value is larger, otherwise this preset value is less.If sample number is 92, preset value can be 92.
Step S150, determines that the pilot data that sequence number in pilot data row equals pointer parameter is target guiding data, and makes the value of target guiding data equal the value of intermediate data parameter; Can be specifically, making second pilot data be target guiding data, and to make target guiding data be 1142.Then return to step S130.According to the variation of pointer parameter, the pilot data of larger sequence number is carried out to assignment renewal.Repeatedly carry out said process, can to pilot data, carry out assignment respectively.1141,1142,1143,1144 pilot data is classified as: ...
Step S160, makes each virtual sample parameters equal the pilot data corresponding with this virtual sample parameters.After definite pilot data is upgraded, make virtual sample parameters and pilot data carry out data interaction.1141,1142,1143,1144 the virtual sample parameters of virtual sample parameters group is respectively: ....
In the present embodiment, by aforesaid method, can arrange and numbering according to actual sample, the value of pilot data in pilot data row is generated automatically, and the value of pilot data is arranged sequentially, and then can make the occurrence of virtual sample parameters also with the order of being scheduled to, arrange, guarantee the one-to-one relationship of virtual sample parameters and actual sample numbering, reduce the mistake of virtual sample parameters, guarantee to analyze the sample body information of acquisition and the corresponding relation of actual sample.
Certainly, the position according to actual sample in adding model, before step S110, can comprise the step that initial value is preset, by this step, the initial value of pointer parameter can be predefined for to 1 or other data; Certainly, according to actual sample numbering rule and distribution situation, the initial value of all right respective settings intermediate data parameter of step that initial value is preset.
Certainly, when sample comprises common sample and standard model, can adopt respectively aforesaid method to common sample and standard model, to guarantee that the virtual sample parameters corresponding with common sample and the numbering of common sample are consistent, and guarantee that the virtual sample parameters corresponding with standard model and the numbering of standard model are consistent.
On the basis of using method that pcr amplification instrument is provided, the embodiment of the present invention also provides a kind of pcr amplification instrument.This pcr amplification instrument comprises memory module, presets at least one virtual sample parameters group in described memory module, and virtual sample parameters group comprises a plurality of virtual sample parameters.In addition, this pcr amplification instrument also comprises auxiliary storage module, initial assignment module, counting pointer locating module, judge module, follow-up assignment module and modular converter.
Auxiliary storage module presets pointer parameter, intermediate data parameter, at least one pilot data row, and described pilot data row comprise a plurality of pilot data of arranging with predefined procedure; A plurality of described pilot data are corresponding one by one with a plurality of virtual sample parameters.The initial value of described pointer parameter can be 1.
Initial assignment module, for determining that according to predetermined instruction predetermined pilot data is benchmark pilot data, carries out assignment to this benchmark pilot data; Make again pointer parameter equal the sequence number of benchmark pilot data in pilot data row, make intermediate data parameter equal benchmark pilot data, then produce pointer shift order;
Counting pointer locating module, for according to pointer shift order, makes pointer parameter increase by 1, makes intermediate data parameter increase Y.According to pointer shift order, can make pcr amplification instrument loop in a predetermined manner assignment.
Judge module, for judging whether pointer parameter is greater than preset value, if not, produces follow-up assignment directive; If so, produce conversion instruction.Judge module determines whether should end loop process.
Follow-up assignment module, for according to follow-up assignment directive, determines that the pilot data that sequence number in pilot data row equals pointer parameter is target guiding data, and makes the value of target guiding data equal intermediate data parameter; Then produce pointer shift order.
Described modular converter, for making each virtual sample parameters equal the pilot data corresponding with this virtual sample parameters according to conversion instruction.
For the ease of middle data parameters is set, pcr amplification instrument can also comprise input unit, and described input unit is for inputting and set the initial value of intermediate data parameter.
According to the rule of actual sample numbering, described Y can be preset value, and between actual sample numbering, difference is 1 o'clock, and the value of Y can be 1.
Position according to actual sample in adding model, initial assignment module can be defined as benchmark pilot data by the suitable pilot data of sequence number in pilot data row.At actual sample when adding position that model starts most and placing, can pilot data row in the pilot data of sequence number minimum as benchmark pilot data.
This pcr amplification instrument principle of work and working process are identical with the using method of above-mentioned pcr amplification instrument, do not repeat them here.
Should be noted that: the pcr amplification instrument that the embodiment of the present invention provides can be realized above-mentioned using method, but the pcr amplification instrument that above-mentioned using method is not limited to provide by the embodiment of the present invention is implemented.
Applied specific case herein technical scheme provided by the invention is set forth, the explanation of above embodiment is just for helping to understand technical scheme provided by the invention.It should be pointed out that for those skilled in the art, under the premise without departing from the principles of the invention, can also carry out some improvement and modification to the present invention; These improvement and modification also fall in the protection domain of the claims in the present invention.
Claims (5)
1. a using method for Polymerase Chain Reaction pcr amplification instrument, described pcr amplification instrument comprises memory module and auxiliary storage module, presets at least one virtual sample parameters group in described memory module, virtual sample parameters group comprises a plurality of virtual sample parameters; Described auxiliary storage module presets pointer parameter, intermediate data parameter, at least one pilot data row, and described pilot data row comprise a plurality of pilot data of arranging with predefined procedure; A plurality of described pilot data are corresponding one by one with a plurality of virtual sample parameters;
It is characterized in that, this using method comprises:
Step S110, determines that according to predetermined instruction predetermined pilot data is benchmark pilot data, carries out assignment to this benchmark pilot data;
Step S120, makes pointer parameter equal the sequence number of benchmark pilot data in pilot data row, and makes intermediate data parameter equal benchmark pilot data;
Step S130, makes pointer parameter increase by 1, makes intermediate data parameter increase Y;
Step S140, judges whether pointer parameter is greater than preset value, if not, enters step S150; If so, enter step S160;
Step S150, determines that the pilot data that sequence number in pilot data row equals pointer parameter is target guiding data, and makes the value of target guiding data equal the value of intermediate data parameter; Return to step S130;
Step S160, makes each virtual sample parameters equal the pilot data corresponding with this virtual sample parameters;
Described pcr amplification instrument, also comprises:
Auxiliary storage module, presets pointer parameter, intermediate data parameter, at least one pilot data row, and described pilot data row comprise a plurality of pilot data of arranging with predefined procedure; A plurality of described pilot data are corresponding one by one with a plurality of virtual sample parameters;
Initial assignment module, for determining that according to predetermined instruction predetermined pilot data is benchmark pilot data, carries out assignment to this benchmark pilot data; Make again pointer parameter equal the sequence number of benchmark pilot data in pilot data row, make intermediate data parameter equal benchmark pilot data, then produce pointer shift order;
Counting pointer locating module, for according to pointer shift order, makes pointer parameter increase by 1, makes intermediate data parameter increase Y;
Judge module, for judging whether pointer parameter is greater than preset value, if not, produces follow-up assignment directive; If so, produce conversion instruction;
Follow-up assignment module, for according to follow-up assignment directive, determines that the pilot data that sequence number in pilot data row equals pointer parameter is target guiding data, and makes the value of target guiding data equal intermediate data parameter; Then produce pointer shift order;
Modular converter, for making each virtual sample parameters equal the pilot data corresponding with this virtual sample parameters according to conversion instruction.
2. the using method of pcr amplification instrument according to claim 1, is characterized in that, before described step S110, also comprises the step S100 that initial value is preset; Step S100 comprises:
The initial value that makes pointer parameter is 1.
3. the using method of pcr amplification instrument according to claim 2, is characterized in that, step S100 also comprises:
Set the initial value of intermediate data parameter.
4. according to the using method of the pcr amplification instrument described in claims 1 to 3 any one, it is characterized in that,
In step S130, make intermediate data parameter increase by 1.
5. the using method of pcr amplification instrument according to claim 4, is characterized in that, in step S110, predetermined pilot data is the pilot data of sequence number minimum in pilot data row.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201210314149.8A CN102816686B (en) | 2012-08-29 | 2012-08-29 | PCR (polymerase chain reaction) amplification instrument and application method thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201210314149.8A CN102816686B (en) | 2012-08-29 | 2012-08-29 | PCR (polymerase chain reaction) amplification instrument and application method thereof |
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| CN102816686A CN102816686A (en) | 2012-12-12 |
| CN102816686B true CN102816686B (en) | 2014-08-20 |
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| CN2767454Y (en) * | 2004-07-06 | 2006-03-29 | 北京工业大学 | Application package structure for microfluidic chip for PCR amplification |
| CN1873021A (en) * | 2005-06-01 | 2006-12-06 | 上海棱光技术有限公司 | Working platform of low-density biochip capable of polynary tests in one-off |
| DE102007029285A1 (en) * | 2007-06-22 | 2008-12-24 | Dspace Digital Signal Processing And Control Engineering Gmbh | Test device for testing at least one electronic control system and method for operating a test device |
| EP2339353B1 (en) * | 2009-12-23 | 2013-07-24 | F. Hoffmann-La Roche AG | Analysis system and computer implemented method for analyzing biological samples |
| CN102253671B (en) * | 2010-05-20 | 2014-10-15 | 上海宝信软件股份有限公司 | Generation device of test data of process control computer system of metallurgy |
| CN102270127B (en) * | 2011-05-30 | 2014-04-16 | 桂林欧博仪器技术有限公司 | Method for programming and controlling flow of analytical instrument |
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