CN102793891B - Traditional Chinese medicine composition having functions of reducing blood fat and resisting hyperuricemia - Google Patents
Traditional Chinese medicine composition having functions of reducing blood fat and resisting hyperuricemia Download PDFInfo
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Abstract
The invention discloses a traditional Chinese medicine composition having functions of reducing blood fat and resisting hyperuricemia. The traditional Chinese medicine composition comprises: by weight, 1 to 5 parts of propolis, 1 to 5 parts of curcuma extract and 1 to 5 parts of rhizoma alismatis extract. The invention also discloses a soft capsule loaded with the traditional Chinese medicine composition having functions of reducing blood fat and resisting hyperuricemia. The soft capsule comprises: by weight, 1 to 5 parts of propolis, 1 to 5 parts of curcuma extract, 1 to 5 parts of rhizoma alismatis extract and 3 to 15 parts of soybean oil. Through the synergism of propolis, curcuma extract and rhizoma alismatis extract, the traditional Chinese medicine composition provided by the invention has functions of reducing blood fat and resisting hyperuricemia.
Description
Technical field
The present invention relates to field of medicinal compositions, be specifically related to the Chinese medicine composition of the anti-metabolic arthritis of a kind of blood fat reducing.
Background technology
Hyperlipemia means lipid concentration overrun in the blood plasma.Since in the blood plasma lipid most of with blood plasma in protein bound, so primary disease is called hyperlipoproteinemia again, is commonly called as hyperlipidemia.Blood fat comprises lipoids and fat, and lipoids mainly is phospholipid, glycolipid, sterin and steroid; Fat mainly is triglyceride.Hyperlipidemia is the main hazard factor that causes human tremulous pulse medicated porridge sample disease.In recent years, along with the raising day by day of people's living standard, the variation of dietary structure and life style, crowd's blood lipid level is raising generally year by year.Blood fat is too high, causes multiple diseases such as coronary heart disease, apoplexy, hypertension, diabetes, liver cirrhosis easily.Therefore, the attenuating serum lipid concentrations is very important for the health of human body.
Hyperuricemia is to be occurred causing purine metabolic disturbance unusually by xanthine oxidase activity, thereby makes uricopoiesis too much or the metabolic disease due to the renal excretion obstacle, and its serious consequence can cause gouty arthritis and uric acid renal damage.In recent years, along with the raising of people's living standard and the change of dietary structure, the quickening of social rhythm and the minimizing of movement time, the hyperuricemia prevalence is worldwide in rising trend.Discover that diseases such as hyperuricemia and cardiovascular and cerebrovascular disease, diabetes, blood fat disorder, obesity are closely related.Therefore, the control of hyperuricemia is also more paid attention to.
Propolis is the dry secretions of Apidae insecticide apis mellifera Linnaeus Apis mellifera L., and the effect that has blood fat reducing preferably clinically and improve immune function of human body now extensively is used to prepare various health product and Chinese medicine preparation.The traditional Chinese medical science thinks that propolis has a little less than the tonify deficiency, changes turbid fat, the function of only quenching one's thirst, and clinical in the empty senilism of body, hyperlipemia is quenched one's thirst.As disclosing a kind of pharmaceutical composition of propolis-containing among the Chinese patent ZL201110075192.9, mainly formed by the raw material of following weight portion: propolis 5 weight portions, Radix Panacis Quinquefolii extract 1-5 weight portion, Radix Notoginseng extract 1-5 weight portion, soybean oil 10-20 weight portion.And a kind of preparation method of this pharmaceutical composition, step comprises: propolis is pulverized, obtained particle diameter less than 80 purpose propolis powder; To be heated to fusion in shortening and the vitamin E adding soybean oil, add propolis powder, Radix Panacis Quinquefolii extract and Radix Notoginseng extract again, mix homogeneously, the gained mixed liquor is crossed 80 mesh sieves after grinding, pelleting (time earlier with gelatin, glycerol, 80 ℃ of melten gels of pure water) again, wash ball, drying is namely made propolis Chinese medicine composition composition soft capsule.The effect that this pharmaceutical composition has blood fat reducing and improves immunity of organisms.
Rhizoma Curcumae Longae is the dry rhizome of zingiberaceous plant Rhizoma Curcumae Longae Curcuma longa L., and acrid in the mouth, hardship, temperature are returned spleen, Liver Channel, are the drug for invigorating blood circulation and eliminating stasis of using always, have the effect of removing blood stasis circulation of qi promoting, inducing menstruation to relieve menalgia.Clinical treatment of aching etc. for rheumatic arthralgia, tumbling and swelling, the contracture of muscle arteries and veins, joint.Tcm clinical practice normal with the treatment of other drug matching for uric acid resisting and gout.
Rhizoma Alismatis is the dry tuber of Alismataceae plant Rhizoma Alismatis Alisma orientalis (Sam.) Juzep..Have diuresis, the function of clearing away damp-heat cures mainly dysuria, edema distension, the oliguria of having loose bowels, dizziness due to fluid-retention, the puckery pain of pyretic stranguria, hyperlipidemia.Tcm clinical practice normal with the treatment of other drug matching for blood fat reducing and gout.
A kind of Chinese medicine composition for reducing blood uric acid is disclosed among the Chinese patent application CN200910098170.7, comprise Rhizoma Smilacis Glabrae or Engelhardtia roxburghiana extract, Rhizoma Alismatis extract and common carrier or excipient, Rhizoma Smilacis Glabrae extract or Engelhardtia roxburghiana extract contain flavones ingredient, astilbin, different astilbin, Quercetin, Quercetin glycosides etc., its total content 50%-98%, Rhizoma Alismatis extract is the extract of the ethanol preparation of employing 30%-100%.This Chinese medicine composition is composite by Rhizoma Smilacis Glabrae extract or Engelhardtia roxburghiana extract and Rhizoma Alismatis extract, has the effect in dehumidifying, detoxifcation, sharp joint, is mainly used in gouty arthritis and hyperuricemia.
The application of flavonoid monomeric compound curcumin in the preparation medicament for restraining uric acid transportor URAT 1 disclosed among the Chinese patent ZL200710190135.9, it is mainly studied the pharmacologically active of the curcumin single component in the Rhizoma Curcumae Longae, the pharmacologically active of the Rhizoma Curcumae Longae extract of complexity and Rhizoma Curcumae Longae extract and other medical material composite is not cooked any research.
A kind of medicine with effect for reducing fat and treatment fatty liver is disclosed among the Chinese patent application CN 200410104293.4, made by following weight percentages: Rhizoma Alismatis 20-99%, Rhizoma Curcumae Longae 1-80%, effect with good effect for reducing fat and treatment fatty liver, toxic and side effects is little, is suitable for the big production of industry.
Because the composition of Chinese crude drug is complicated, and the very difficult prediction of various Chinese crude drug composite effect each other, therefore, people are also constantly exploring the Chinese medicine composition with good drug effect.
Summary of the invention
The invention provides the Chinese medicine composition of the anti-metabolic arthritis of a kind of blood fat reducing, utilize the synergism of propolis, Rhizoma Curcumae Longae extract and Rhizoma Alismatis extract, effectively blood fat reducing and effectively reduction metabolic arthritis.
The Chinese medicine composition of the anti-metabolic arthritis of a kind of blood fat reducing is made up of 1-5 weight portion propolis, 1-5 weight portion Rhizoma Curcumae Longae extract and 1-5 weight portion Rhizoma Alismatis extract, preferably is made up of 1 weight portion propolis, 2 weight portion Rhizoma Curcumae Longae extracts and 2 weight portion Rhizoma Alismatis extracts.
Described Chinese medicine composition can be used for preparing health product or the medicine of blood fat reducing and/or anti-metabolic arthritis.
The preparation method of described Chinese medicine composition comprises step:
Propolis is pulverized, obtained the propolis powder; With propolis powder, Rhizoma Curcumae Longae extract and Rhizoma Alismatis extract mix homogeneously, obtain Chinese medicine composition.
Described Chinese medicine composition can add any dosage form of making behind the pharmaceutic adjuvant in tablet, capsule, the granule etc.
A kind of Chinese medicine composition composition soft capsule comprises 1-5 weight portion propolis, 1-5 weight portion Rhizoma Curcumae Longae extract, 1-5 weight portion Rhizoma Alismatis extract and 3-15 weight portion soybean oil; Preferably include 1 weight portion propolis, 2 weight portion Rhizoma Curcumae Longae extracts, 2 weight portion Rhizoma Alismatis extracts and 3-5 weight portion soybean oil.
Also can add an amount of shortening and vitamin E in the described soybean oil.
Described shortening as suspending agent, helps the suspendible of Chinese medicine and soybean oil in the Chinese medicine composition composition soft capsule; The amount that shortening adds is generally the 3%-12% of soybean oil quality.
Described vitamin E Wheat Protein is conducive to improve the quality stability of Chinese medicine composition soft capsule preparation; The amount that vitamin E adds is generally the 0.1%-0.5% of soybean oil quality.
Described Chinese medicine composition preparation of soft capsule method may further comprise the steps:
(1) propolis is pulverized, obtained particle diameter less than 80 purpose propolis powder;
(2) with being heated to fusion in shortening and the vitamin E adding soybean oil, add propolis powder, Rhizoma Curcumae Longae extract and Rhizoma Alismatis extract in the step (1) again, mix homogeneously, the gained mixed liquor is crossed 80 mesh sieves after grinding, pelleting is washed ball again, drying is namely made the Chinese medicine composition composition soft capsule.
In the step (2), described heating-up temperature is generally 30 ℃-40 ℃.
Described Chinese medicine composition composition soft capsule can be used as or for the preparation of health product or the medicine of blood fat reducing and/or anti-metabolic arthritis disease.
Described propolis can adopt the commercially available prod, also can prepare voluntarily, its preparation method can carry out with reference to the preparation method of the propolis of record in " medicinal herb components chemistry ", specifically comprise step: concentrated, the vacuum drying of propolis raw material pulverizing, soak with ethanol, cold preservation, filtration, filtrate with plucking namely gets propolis.
Described Rhizoma Curcumae Longae extract can adopt the commercially available prod, also can prepare voluntarily, its preparation method can carry out with reference to the record method in " medicinal herb components chemistry ", specifically comprise step: with Rhizoma Curcumae Longae pulverizing, alcohol reflux, cold preservation, filtration, concentrated, the vacuum drying of filtrate, namely get Rhizoma Curcumae Longae extract.Rhizoma Curcumae Longae extract of the present invention is equivalent to a thirtieth to 1/10th of Rhizoma Curcumae Longae raw medicinal herbs quality.
Described Rhizoma Alismatis extract can adopt the commercially available prod, also can prepare voluntarily, its preparation method can carry out with reference to the record method in " medicinal herb components chemistry ", specifically comprise step: with Rhizoma Alismatis pulverizing, alcohol reflux, cold preservation, filtration, concentrated, the vacuum drying of filtrate, namely get Rhizoma Alismatis extract.Rhizoma Alismatis extract of the present invention is equivalent to a thirtieth to 1/10th of Rhizoma Alismatis raw medicinal herbs quality.
Described propolis, Rhizoma Curcumae Longae and Rhizoma Alismatis medical material all meet the quality standard of propolis in the Pharmacopoeia of the People's Republic of China, Rhizoma Curcumae Longae and Rhizoma Alismatis.Propolis is the dry secretions of Apidae insecticide apis mellifera Linnaeus Apis melliferaL..Rhizoma Curcumae Longae is the dry rhizome of zingiberaceous plant Rhizoma Curcumae Longae Curcuma longaL..Rhizoma Alismatis is the dry tuber of Alismataceae plant Rhizoma Alismatis Alisma orientalis (Sam.) Juzep..
The present invention has following beneficial effect:
Chinese medicine composition of the present invention utilizes propolis, Rhizoma Curcumae Longae extract and Rhizoma Alismatis extract three's synergism, has good hypolipemic function, compares the effect that also has anti-metabolic arthritis disease, enhancing immunity with existing blood lipid-lowering medicine.Simultaneously, this three flavors Chinese medicine synergism has significantly improved the effect of effect for reducing blood fat and the anti-metabolic arthritis of single medicinal material, hyperlipidemia and/or metabolic arthritis disease crowd are had good health-care effect and therapeutical effect, still do not have the product of this prescription at present both at home and abroad, the present invention has filled up this blank.
The specific embodiment
Propolis is purchased in nectar source, kitchen, sky, Hangzhou health product company limited, general flavone content 〉=20%.
Rhizoma Curcumae Longae extract is purchased the brilliant development in science and technology company limited difficult to understand in Xi'an, and crude drug and Rhizoma Curcumae Longae extract mass ratio are 20: 1, ash＜5%, loss on drying≤5.0%.
Rhizoma Alismatis extract is purchased the brilliant development in science and technology company limited difficult to understand in Xi'an, and crude drug and Rhizoma Alismatis extract mass ratio are 20: 1, ash＜5%, loss on drying≤5.0%.
Embodiment 1
Propolis is pulverized, obtained particle diameter less than 80 purpose propolis powder.
To be heated to fusion in 30 ℃ in 15g shortening and the 0.5g vitamin E adding 484.5g soybean oil, add 100g propolis powder, 200g Rhizoma Alismatis extract and 200g Rhizoma Curcumae Longae extract again, mix homogeneously, the gained mixed liquor is crossed 80 mesh sieves after grinding, be that the capsule shell material carries out pelleting with gelatin and glycerol again, wash ball, drying is made the Chinese medicine composition composition soft capsule.
Embodiment 2
Propolis is pulverized, obtained particle diameter less than 80 purpose propolis powder;
To be heated to fusion in 40 ℃ in 9g shortening and the 0.3g vitamin E adding 290.7g soybean oil, add 100g Rhizoma Alismatis extract, 100g Rhizoma Curcumae Longae extract, 100g propolis powder mix homogeneously again, the gained mixed liquor is crossed 80 mesh sieves after grinding, be that the capsule shell material carries out pelleting with gelatin and glycerol again, wash ball, drying is made the Chinese medicine composition composition soft capsule.
Embodiment 3
Propolis is pulverized, obtained particle diameter less than 80 purpose propolis powder;
To be heated to fusion in 40 ℃ in 21g shortening and the 0.7g vitamin E adding 678.3g soybean oil, add 100g propolis powder, 300g Rhizoma Alismatis extract and 300g Rhizoma Curcumae Longae extract again, mix homogeneously, the gained mixed liquor is crossed 80 mesh sieves after grinding, be that the capsule shell material carries out pelleting with gelatin and glycerol again, wash ball, drying is made the Chinese medicine composition composition soft capsule.
Embodiment 4
Propolis is pulverized, obtained particle diameter less than 80 purpose propolis powder;
To be heated to fusion in 40 ℃ in 15g shortening and the 0.5g vitamin E adding 484.5g soybean oil, add 300g propolis powder, 100g Rhizoma Alismatis extract and 100g Rhizoma Curcumae Longae extract again, mix homogeneously, the gained mixed liquor is crossed 80 mesh sieves after grinding, be that the capsule shell material carries out pelleting with gelatin and glycerol again, wash ball, drying is made the Chinese medicine composition composition soft capsule.
Embodiment 5
Propolis is pulverized, obtained particle diameter less than 80 purpose propolis powder.
With 200g Rhizoma Alismatis extract, 200g Rhizoma Curcumae Longae extract and 100g propolis powder mix homogeneously, make Chinese medicine composition.
Embodiment 6
Propolis is pulverized, obtained particle diameter less than 80 purpose propolis powder.
With 400g Rhizoma Alismatis extract, 400g Rhizoma Curcumae Longae extract and 100g propolis powder mix homogeneously, make Chinese medicine composition.
Embodiment 7
Propolis is pulverized, obtained particle diameter less than 80 purpose propolis powder.
With 500g Rhizoma Alismatis extract, 500g Rhizoma Curcumae Longae extract and 100g propolis powder mix homogeneously, make Chinese medicine composition.
Embodiment 8
Propolis is pulverized, obtained particle diameter less than 80 purpose propolis powder.
With 100g Rhizoma Alismatis extract, 100g Rhizoma Curcumae Longae extract and 200g propolis powder mix homogeneously, make Chinese medicine composition.
Embodiment 9
Propolis is pulverized, obtained particle diameter less than 80 purpose propolis powder.
With 100g Rhizoma Alismatis extract, 100g Rhizoma Curcumae Longae extract and 400g propolis powder mix homogeneously, make Chinese medicine composition.
Embodiment 10
Propolis is pulverized, obtained particle diameter less than 80 purpose propolis powder.
With 100g Rhizoma Alismatis extract, 100g Rhizoma Curcumae Longae extract and 500g propolis powder mix homogeneously, make Chinese medicine composition.The research of Chinese medicine composition main pharmacodynamics
Chinese medicine composition is made up of propolis, Rhizoma Curcumae Longae extract and Rhizoma Alismatis extract, by to animal blood fat reducing and anti-metabolic arthritis experimental study of effect, observes the drug action of Chinese medicine composition.Now its Pharmacodynamic test of active extract result is reported as follows.
1 materials and methods
1.1 material
1.1.1 sample character: the Chinese medicine composition composition soft capsule is the brown oil paste (soft capsule content) of embodiment 1 preparation, be the Chinese medicine composition of embodiment 1 preparation, this experiment is that the Chinese medicine composition that solvent prepares embodiment 1 is mixed with each dosage for examination with the soybean oil.The raw material that propolis, Rhizoma Curcumae Longae extract and Rhizoma Alismatis extract provide for embodiment 1.
1.1.2 laboratory animal SD rat is male, the cleaning level, and body weight 160g-180g is provided by Shanghai Slac Experimental Animal Co., Ltd., and the laboratory animal production licence is: SCXK (Shanghai) 2007-2005.Animal feed is provided by Zhejiang Province's Experimental Animal Center, operative norm GB14924-2001.Sense environmental conditions, experimental animal room credit number are SCXK (Shanghai) 2009-0059, temperature range 20-24 ℃, and relative humidity scope 40%-70%.In the Animal House environment, adapt to 3 days before the rat test.
1.1.3 main agents: cholesterol determination test kit, enzyme process triglyceride determination test kit, determine cholesterol with high density lipoprotein test kit (Shanghai Foxing Changzheng medical science Co., Ltd).
1.1.4 key instrument TBA-40FR type automatic clinical chemistry analyzer (Toshiba), centrifuge, adjustable pipette etc.
1.1.5 high lipid food: 78.8% normal feedstuff, 1% cholesterol, 10% egg albumen powder, 10% Adeps Sus domestica and 0.2% cholate, mass percent.
1.2 method
1.2.1 grouping experiment is established basic, normal, high three the dosage groups of Chinese medicine composition composition soft capsule: 0.17g/kg/ day, 1.00g/kg/ day, 2.00g/kg/ day, be equivalent to respectively 5,30,60 times (Chinese medicine composition people recommended amounts of the present invention is the 2g/60kg body weight/day) of the actual intake of people.Other establishes propolis group, Rhizoma Alismatis extract group, Rhizoma Curcumae Longae extract group, water matched group and high fatty oil matched group (soybean oil).Take by weighing Chinese medicine composition soft capsule content 3.33g, 20.00g, 40.00g respectively, to 200ml, be made into 1.70g/100ml, 10.00g/100ml, three concentration of 20.00g/100ml with soybean oil.All using soybean oil (containing shortening and vitamin E) among the embodiment 1 to be mixed with mass percentage concentration earlier propolis, Rhizoma Curcumae Longae extract and Rhizoma Alismatis extract is 50%, take by weighing 20.00g more respectively, to 200ml, be made into 10.00g/100ml concentration, as test liquid with soybean oil.Irritate the stomach volume and be the 1ml/100g body weight.
1.2.2 experimental technique adopts the preventative given the test agent of giving.Rat feeding normal feedstuff observation 8d under experimental situation (my god), serum total cholesterol (TC), serum levels of triglyceride (TG), serum high-density LP cholesterol (HDL-C) level are measured in the blood sampling of eye socket venous plexus.According to serum total cholesterol (TC) level, be divided into 8 groups at random, 10 every group, be respectively high fat water matched group, high fatty oil matched group and respectively tried the thing group.Each experimental group all gives high lipid food, tried thing and respectively organized per os filling stomach to being subjected to test solution, high fat water matched group and high fatty oil matched group per os are respectively irritated stomach to water and soybean oil with volume, and regularly weigh in, fasting 16h when 30d, serum TC, TG, HDL-C level are measured in the blood sampling of eye socket venous plexus.
Represent with x ± s that 1.2.3 respectively organize data mean relatively adopts variance analysis between many groups, all data are handled by SPSS 16.0 statistical softwares, and there is significance P＜0.05 for difference on the statistics.
2 results
2.1 the Chinese medicine composition composition soft capsule is to the influence (table 1) of hyperlipidemia rats body weight.
By table 1 as seen, give and to be tried before the thing and to be tried in the thing process, basic, normal, high three the dosage groups of Chinese medicine composition composition soft capsule, propolis group, Rhizoma Alismatis extract group, Rhizoma Curcumae Longae extract group rat body weight and high fat water matched group and high fatty oil matched group compare, the equal not statistically significant of difference (P＞0.05).
Table 1 Chinese medicine composition composition soft capsule is to the influence of hyperlipidemia rats body weight
2.2 the Chinese medicine composition composition soft capsule is to the influence (table 2) of hyperlipidemia rats serum TC.
By table 2 as seen, Serum TC value basic value is 2.21 ± 0.36mmol/L before the high fat water matched group test.Behind the feed high lipid food 30d, recording the Serum TC value is 2.80 ± 0.31mmol/L, with the basic value difference statistical significance (P＜0.001) is arranged, and model is set up.Behind the feed high lipid food 30d, high fatty oil control rats serum TC value and high fat water matched group comparing difference not statistically significant (P＞0.05).The high, medium and low dosage group of Chinese medicine composition composition soft capsule, propolis group and high fat water matched group comparison serum TC value all have obvious reduction, and difference all has statistical significance (P＜0.05).And the relatively more equal no significant difference (P＜0.05) of single Rhizoma Alismatis extract, Rhizoma Curcumae Longae extract and high fat water matched group.The result shows that the effect that serum total cholesterol (TC) falls in the Chinese medicine composition composition soft capsule will obviously be better than single medicinal material.
Table 2 Chinese medicine composition composition soft capsule is to the influence of hyperlipidemia rats serum TC content
△ represents to compare P＜0.05 with high fat water matched group.
2.3 the Chinese medicine composition composition soft capsule is to the influence (table 3) of hyperlipidemia rats serum TG.
By table 3 as seen, rat blood serum TG value basic value is 0.90 ± 0.18mmol/L before the high fat water matched group test.Behind the feed high lipid food 30d, recording rat blood serum TG value is 1.39 ± 0.27mmol/L, with the basic value difference statistical significance (P＜0.001) is arranged, and model is set up.After feeding high lipid food 30d, high fatty oil control rats serum TG and the equal not statistically significant of high fat water control rats comparing difference (P＞0.05).The Chinese medicine composition composition soft capsule is middle and high, low dose group rat blood serum TG value and high fat water matched group more all have obvious reduction, significant difference (P＜0.05).And each group of single medicinal material does not relatively have obvious reduction (P＞0.05) with high fat water matched group.The result shows that the effect that serum levels of triglyceride (TG) falls in the Chinese medicine composition composition soft capsule will obviously be better than single medicinal material.
Table 3 Chinese medicine composition composition soft capsule is to the influence of hyperlipidemia rats serum TG
△ represents to compare P＜0.05 with high fat water matched group.
2.4 the Chinese medicine composition composition soft capsule is to the influence (table 4) of hyperlipidemia rats Serum HDL-C.
By table 4 as seen, behind the feed high lipid food 30d, high fatty oil control rats Serum HDL-C and high fat water control rats comparing difference not statistically significant (P＞0.05).Chinese medicine composition composition soft capsule height, middle dosage group hyperlipidemia rats Serum HDL-C value and high fat water matched group relatively have obvious rising, significant difference (P＜0.05).And each group of single medicinal material and the relatively more equal no significant difference (P＞0.05) of high fat water matched group.The result shows that the Chinese medicine composition composition soft capsule can make serum high-density LP cholesterol (HDL-C) obviously rise (P＜0.05), and each group of single medicinal material does not all have obviously rise (P＞0.05).
Table 4 Chinese medicine composition composition soft capsule is to the influence of hyperlipidemia rats Serum HDL-C content
△ represents to compare P＜0.05 with high fat water matched group.
3. conclusion
Compare with high fat water matched group, the basic, normal, high dosage group of Chinese medicine composition of the present invention all can obviously reduce hyperlipidemia rats serum total cholesterol (TC) content.Basic, normal, high three the dosage groups of Chinese medicine composition of the present invention all can obviously reduce hyperlipidemia rat blood serum triglyceride (TG) content, and difference has statistical significance.Chinese medicine composition composition soft capsule height of the present invention, middle dosage group rat blood serum HDL-C (HDL-C) content and high fat water control rats are compared obvious rising, and difference has statistical significance.And propolis, Rhizoma Curcumae Longae extract and three single medicinal material groups of Rhizoma Alismatis extract only propolis have outside the effect (P＜0.05) of obvious reduction TC, other each group is to the effect of TC, TG, HDL-C all not obvious (P＞0.05).The result shows that the effect that the Chinese medicine composition composition soft capsule falls serum total cholesterol (TC), serum levels of triglyceride (TG) and promotes HDL-C (HDL-C) will obviously be better than propolis, Rhizoma Curcumae Longae extract and three single medicinal materials of Rhizoma Alismatis extract.
Conclusion: Chinese medicine composition composition soft capsule of the present invention has the effect of blood fat reducing.
The anti-mice metabolic arthritis of Chinese medicine composition experimental study of effect
1 materials and methods
1.1 material
1.1.1 sample character: the Chinese medicine composition composition soft capsule is the brown oil paste (soft capsule content) of embodiment 1 preparation, be that solvent is mixed with each dosage for examination with Chinese medicine composition with the soybean oil, namely take by weighing respectively for test agent 1.0g, 6.0g and 12.0g with soybean oil to 60ml, being mixed with concentration is 0.017g/ml, 0.100g/ml and 0.200g/ml sample, as test liquid.With embodiment 1 provide raw material---it is 50% that propolis, Rhizoma Curcumae Longae extract, Rhizoma Alismatis extract all use the soybean oil (containing shortening and vitamin E) among the embodiment 1 to be mixed with the crude drug mass percentage concentration earlier, take by weighing 20.00g more respectively, with soybean oil to 200ml, be made into 10.00g/100ml concentration, as test liquid.Irritate the stomach volume and be the 0.1ml/10g body weight.
1.1.2 allopurinol tablet: the 0.1g/ sheet, Jiangsu side forces pharmaceutical factory Co., Ltd to produce lot number 201110302.
1.2 laboratory animal and testing conditions:
ICR kind mice, male and female half and half, body weight 18-22 gram provides licence by west, Shanghai pul-Bi Kai laboratory animal company limited: SCXK (Shanghai) 2008-0016.Animal feed is provided by Zhejiang Province's Experimental Animal Center, operative norm GB14924-2001.Sense environmental conditions, 20 ℃-23 ℃ of temperature ranges, relative humidity scope 40%-70%.In the Animal House environment, adapt to 5 days before the mouse test.
1.3 key instrument and reagent:
Electronic balance, 721 spectrophotometers, constant water bath box, operating theater instruments etc.Yeast diffusion juice is gone up the production of sea blue season development in science and technology company limited, lot number: 111008.The ebutol sheet, Hangzhou Minsheng Medcine Co., Ltd, lot number: T11G515.The uric acid detection kit, Sai Ke Bioisystech Co., Ltd in Ningbo produces, lot number: 20120412.Xanthine oxidase (XOD) testing cassete, Nanjing is built up bio-engineering research and is produced, lot number: 20120419.
1.4 experimental technique:
1.4.1 totally 90 of grouping experiment mices, adaptability was raised after 5 days, be divided into 9 groups at random, every group 10, male and female half and half, if three dosage groups of Chinese medicine composition composition soft capsule, Rhizoma Alismatis extract group (1.00g/kg), Rhizoma Curcumae Longae extract group (1.00g/kg), positive controls (allopurinol tablet), model control group (distilled water), solvent control group (soybean oil) are established the normal control group simultaneously.Basic, normal, high three the dosage groups of Chinese medicine composition composition soft capsule are respectively 0.17,1.00, the 2.00g/kg/ daily weight, are equivalent to 5,30 and 60 times (people's recommended amounts is 2.0g/60kg/ day) of people's recommended amounts.Positive controls is irritated stomach allopurinol tablet 0.3g/kg body weight, is equivalent to 30 times (it is 0.01g/60kg/ day that the people recommends maximum amount) of people's recommended amounts.Each group irritates that stomach is corresponding to be tried thing totally 21 days, wherein playing 1-8 on the 8th day organizes and irritates stomach 30g/kg Yeast diffusion juice and 0.25g/kg ethambutol respectively every day, continuous 14 days, cause the metabolic arthritis mouse model, each group continues to irritate the corresponding thing that tried of stomach simultaneously during the modeling, and raise routinely, claim body weight weekly one time.
1.4.2 index determining method: after the last administration, mice is 24h on an empty stomach, plucks eyeball and gets blood, places 1h, and the centrifugal 10min of 2000r/min collects serum, presses the test kit description and measures uric acid content in the serum.Take off cervical vertebra and put to death mice, get liver organization, homogenate, the centrifugal 10min of 3500r/min gets supernatant, presses the test kit description and measures XOD.
1.5 experimental data statistics: adopt the SPSS16.0 statistical software to analyze, data all with
Expression is organized mean more and is relatively adopted the F check, and wherein two groups of relatively measurement data employing LSD-t checks add during heterogeneity of variance and do Dunnett T3 check.
2, result and analysis
2.1 the Chinese medicine composition composition soft capsule is to the influence (seeing Table 5) of metabolic arthritis model mice body weight
Table 5: the Chinese medicine composition composition soft capsule is to the influence of metabolic arthritis model mice body weight
▲ expression is compared P＜0.05 with the normal control group.
By table 5 as seen, each group of Chinese medicine composition composition soft capsule and other are tried thing and are respectively organized mice body weight and model contrast, solvent control group, the comparison of normal control group there are no significant difference (P＞0.05), and allopurinol group and normal control group are relatively, body weight obviously descend (P＜0.05).Show that allopurinol has a significant effect to the mice body weight.
2.2 the Chinese medicine composition composition soft capsule is to the influence (seeing Table 6) of metabolic arthritis model mice SUA, XOD content
Table 6 Chinese medicine composition composition soft capsule is to the influence of metabolic arthritis model mice SUA, XOD content
△ represents to compare P＜0.05 with model control group.
By table 6 as seen, the high, medium and low dosage group of Chinese medicine composition serum uric acid (SUA) content and model control group relatively significance descend (P＜0.05), and single medicinal material group SUA and model control group relatively there are no significant descend (P＞0.05).Show that the Chinese medicine composition composition soft capsule has the effect that reduces the mouse blood metabolic arthritis.Be also shown in the active and apparent in view rising of normal control group (P＜0.05) of model control group Mouse Liver XOD by table 6.The XOD of the high, medium and low dosage group of Chinese medicine composition composition soft capsule, allopurinol group is active significantly to reduce (P＜0.05) with model group.The single medicinal material group also can make that Mouse Liver XOD is active to be reduced, but not significantly (P＞0.05).Show that heavy dose of filling stomach Yeast diffusion juice and ethambutol make uric acid rising in the mice body, liver XOD is active to be increased, and Chinese medicine composition composition soft capsule and allopurinol have the effect of inhibition XOD activity.
3, discuss
Experimental result shows: the Chinese medicine composition composition soft capsule is respectively organized mice body weight and model contrast, solvent control group, normal control group relatively there are no significant difference (P＞0.05), and allopurinol group and the comparison of normal control group, body weight obviously descend (P＜0.05).Show that allopurinol has a significant effect to the mice body weight, this may have big side effect with allopurinol, and as vomiting, inappetence due to the GI irritation, hepatorenal damage etc. are relevant.Model contrast and solvent control group mice serum uric acid (SUA) content and the apparent in view rising of normal control group (P＜0.05) show the modeling success.The high, medium and low dosage group of Chinese medicine composition composition soft capsule serum uric acid (SUA) content and model control group relatively significance descend (P＜0.05), show that the Chinese medicine composition composition soft capsule has the effect that reduces the mouse blood metabolic arthritis.Active and the apparent in view rising of normal control group (P＜0.05) of model control group and solvent control group Mouse Liver XOD; The XOD of each dosage group of Chinese medicine composition composition soft capsule and allopurinol group is active significantly to reduce (P＜0.05) with model group, shows that Chinese medicine composition composition soft capsule and allopurinol have the effect that suppresses the XOD activity.Single medicinal material Rhizoma Curcumae Longae extract, Rhizoma Alismatis extract all have certain effect of falling serum uric acid (SUA) regulating liver-QI xanthine oxidase (XOD), but act on not remarkable (P＞0.05).The result shows that the Chinese medicine composition composition soft capsule has the effect of obvious reduction metabolic arthritis model mice blood uric acid and liver xanthine oxidase activity, and its effect will obviously be better than single medicinal material---Rhizoma Alismatis extract, Rhizoma Curcumae Longae extract.
Conclusion: Chinese medicine composition composition soft capsule of the present invention has the effect of anti-metabolic arthritis.
Claims (6)
1. the Chinese medicine composition of the anti-metabolic arthritis of blood fat reducing is characterized in that, is made up of 1 weight portion propolis, 2 weight portion Rhizoma Curcumae Longae extracts and 2 weight portion Rhizoma Alismatis extracts;
The preparation process of described Rhizoma Curcumae Longae extract is: with Rhizoma Curcumae Longae pulverizing, alcohol reflux, cold preservation, filtration, concentrated, the vacuum drying of filtrate;
The preparation process of described Rhizoma Alismatis extract is: with Rhizoma Alismatis pulverizing, alcohol reflux, cold preservation, filtration, concentrated, the vacuum drying of filtrate.
2. the application of Chinese medicine composition according to claim 1 is characterized in that, described Chinese medicine composition is in preparation blood fat reducing and/or the health product of anti-metabolic arthritis or the application in the medicine.
3. the Chinese medicine composition composition soft capsule of the anti-metabolic arthritis of blood fat reducing is characterized in that, is made up of 1 weight portion propolis, 2 weight portion Rhizoma Curcumae Longae extracts, 2 weight portion Rhizoma Alismatis extracts and 3-5 weight portion soybean oil;
The preparation process of described Rhizoma Curcumae Longae extract is: with Rhizoma Curcumae Longae pulverizing, alcohol reflux, cold preservation, filtration, concentrated, the vacuum drying of filtrate;
The preparation process of described Rhizoma Alismatis extract is: with Rhizoma Alismatis pulverizing, alcohol reflux, cold preservation, filtration, concentrated, the vacuum drying of filtrate.
4. Chinese medicine composition composition soft capsule according to claim 3 is characterized in that, is added with shortening and vitamin E in the described soybean oil.
5. Chinese medicine composition preparation of soft capsule method according to claim 4 is characterized in that, may further comprise the steps:
(1) propolis is pulverized, obtained particle diameter less than 80 purpose propolis powder;
(2) with being heated to fusion in shortening and the vitamin E adding soybean oil, add propolis powder, Rhizoma Curcumae Longae extract and Rhizoma Alismatis extract in the step (1) again, mix homogeneously, the gained mixed liquor is crossed 80 mesh sieves after grinding, pelleting is washed ball again, drying is made the Chinese medicine composition composition soft capsule.
6. according to the application of each described Chinese medicine composition composition soft capsule of claim 3-4, it is characterized in that described Chinese medicine composition composition soft capsule is in preparation blood fat reducing and/or the health product of anti-metabolic arthritis or the application in the medicine.
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