CN102789591A

CN102789591A - Milk somatic cell counting device based on computer vision

Info

Publication number: CN102789591A
Application number: CN2012101742669A
Authority: CN
Inventors: 高飞; 袁晓阳; 李洪波; 沈国瑜; 张元鸣; 肖刚
Original assignee: Hangzhou Ultrasun Technologies Co ltd; Zhejiang University of Technology ZJUT
Current assignee: Hangzhou Ultrasun Technologies Co ltd; Zhejiang University of Technology ZJUT
Priority date: 2012-05-28
Filing date: 2012-05-28
Publication date: 2012-11-21
Anticipated expiration: 2032-05-28
Also published as: CN102789591B

Abstract

The invention discloses a milk somatic cell counting device based on computer vision. The device comprises a slide moving device, an image capture device, an image analyzing device and a light source which is matched with the image capture device, wherein the slide moving device comprises an upper layer moving platform and a lower layer moving platform, the upper layer platform is driven by a first stepping motor to move to the left and right, the lower layer platform is driven by a second stepping motor to move back and forth, and both the upper layer moving platform and the lower layer moving platform are arranged on an orbit; the image capture device comprises a digital microscope and a supporting fixing part of the digital microscope; the image analyzing device comprises an industrial personal computer, and the industrial personal computer is provided with an image analyzing module; and the digital microscope is connected with the industrial personal computer, the first stepping motor, the second stepping motor and the light source are connected with a single chip microcomputer, and the single chip microcomputer is connected with the industrial personal computer. According to the device, the movement of the side is controlled through control of the movement of the moving platforms, during the moving process, amplified images of milk somatic cells are collected and then analyzed, and the quantity of the milk somatic cells is obtained.

Description

Milk somatic cell counting assembly based on computer vision

Technical field

The present invention relates to the milk somatic cell measuring technique, especially a kind of milk somatic cell method of counting.

Background technology

Body cell in the milk is made up of macrophage, lymphocyte, polymorph neutrophile leucocytes and a spot of breast tissue epithelial cell usually.Somatic number (SCC) is meant the body cell number that contains in every ml milk.

SCC can reflect that the milk milk house receives the degree of bacterial infection, can also be used to estimating the damaed cordition of milk production of cow.When health particularly breast infected or when injuring, body cell can arrive at the injury along with blood.Macrophage is engulfed bacterium with the nuclear neutrophil on the one hand, and lymphocyte is then controlled immune response and produced antibody to resist Bacteria destroyed, plays the effect of protection body; Be embodied on the other hand that somatic quantity obviously increases in the milk of extruding.The udder health of somatic number and milk cow is closely related.Through somatic detection, can weigh the health status of whole cows mammary glandular cell.Therefore can be through detecting the mammitis of the next effectively control of somatic quantity milk cow in the raw milk.When SCC surpasses 5 * 10 ⁵The time, the possibility of milk cow bacterial infection is bigger. and the mammitis incidence of disease is very high.Composition in the corresponding raw milk changes and increases. the degradation of milk.SCC can be used as the major criterion of judging the milk quality height: SCC is low more, and the quality of milk is high more; SCC is high more, and is big more to the influence of milk quality.

Existing detection method mainly contains the inferior mensuration of markon's welfare, fluorescence streaming counting method, electronics particle counting, microscopic method.

SCC can use CMT (the inferior mensuration of markon's welfare) to measure.CMT adds specific surfactant in the milk to, when the cell in the milk runs into surfactant, can shrink and solidify, and makes cell emit nuclifort and aggegation.Cell is many more, and state of aggregation is strong more, and the aggegation sheet of appearance is many more.The CMT method is quick, responsive, and low price, and test method is simple, and equipment needed thereby is few, and the result is comparatively accurate in reflection.But the CMT method is the relative populations of an individual cells, rather than exact magnitude, and thinks that factor affecting is bigger, should have the special training personnel fixedly to do this inspection.

Also can be used for calculating SCC, i.e. fluorescence streaming counting method through measuring chemiluminescence reaction.This method is in milk, to add a kind of fluorescence additive, and this fluorescence additive can be absorbed by cell.Use the rayed milk of specific wavelength then, this moment, cell can send the fluorescence of another kind of characteristic wavelength.Through the appropriate filter of using one can discern this characteristic wavelength, just can calculate the cell number in the milk.This method need be sampled to milk, and the fluorescence additive that need choose appropriate components mixes with it, also need select suitable illumination and filtrator.This is that a workload is big and expend higher process.

SCC also can measure through the electronics particle counting.Method mainly relies on the variation that detects milk electric conductivity in this, and this is because in general, and is higher than the conductivity in the normal milk in the conductivity of mastitis milk.Conductivity has the direct current of probe or the loop of interchange to measure through one usually, and this probe is fixed in the milk flow.This probe is very sensitive.This probe generally comprises two electrodes, and direct current or alternating current are through forming electric current in milk.The variation of milk conductivity is measured through change in current in the loop.Yet colloid can the deposition on electrode cause the inaccurate of reading through regular meeting in the milk.This measuring method also has some shortcomings.This measuring method relies on some variations in the milk, and these only change to have an effect at bacterium and leucocyte and just can take place.So it is not too suitable that this initial stage for mastitis is detected.Simultaneously, the repeatability of this method is also poor, because in difference test or different milk cow, and the difference that electrolyte ingredient and concentration are great.So, use to make separately to diagnose in this way and take a risk.

Microscopic method is as the standard method of body cell counting, and it is generally used for proofreading and correct the correctness of body cell analyser and additive method.But present microscopic method only limits to manually-operated, and manually-operated not only can cause the low of work efficiency, and can produce the error of artificial property inevitably.

Summary of the invention

In order to overcome the existing existing human factor of milk somatic cell counting assembly, detection efficiency is lower, accuracy is relatively poor deficiency, the present invention provides a kind of human factor influence, high, good milk somatic cell counting assembly based on computer vision of accuracy of detection efficiency effectively avoided.

The technical solution adopted for the present invention to solve the technical problems is:

Milk somatic cell counting assembly based on computer vision is characterized in that: comprise the slide telecontrol equipment, and image-taking device, image analysis apparatus cooperates the light source of described image-taking device; Said slide telecontrol equipment comprises upper strata motion platform, lower floor's motion platform; Described upper platform carries out side-to-side movement by the first stepping driven by motor; Described lower floor platform is driven by second stepper motor and seesaws, and described upper strata motion platform, lower floor's motion platform all are provided with on the orbit; Said image-taking device is made up of digit microscope and support fixation elements thereof; Image analysis apparatus is by industrial computer, the image analysis module that described industrial computer is provided with; Described digit microscope connects described industrial computer, and the control end of described first stepper motor, second stepper motor, light source all connects single-chip microcomputer, and described single-chip microcomputer connects described industrial computer.

Thereby this device is controlled the motion to slide through the motion of controlled motion platform, and the somatic enlarged image of collecting milk draws milk somatic cell quantity to image analysis then in motion process.

Advantage of the present invention is: it is slow to have solved conventional microscope method speed, and is subject to the shortcoming of manually-operated influence, has improved accuracy of detection greatly, and can be used for the other types cell count, has vast market prospect and using value.

Description of drawings

Fig. 1 is the slide telecontrol equipment described in the present invention and the vertical view of image-taking device.

Fig. 2 is the slide telecontrol equipment described in the present invention and the side view of image-taking device.

Fig. 3 is the annexation figure between each parts among the present invention.

Embodiment

Below in conjunction with accompanying drawing the present invention is further described.

Milk somatic cell counting assembly based on computer vision comprises the slide telecontrol equipment, image-taking device, and image analysis apparatus cooperates the light source of described image-taking device; Said slide telecontrol equipment comprises upper strata motion platform, lower floor's motion platform; Described upper platform carries out side-to-side movement by the first stepping driven by motor; Described lower floor platform is driven by second stepper motor and seesaws, and described upper strata motion platform, lower floor's motion platform all are provided with on the orbit; Said image-taking device is made up of digit microscope and support fixation elements thereof; Image analysis apparatus is by industrial computer, the image analysis module that described industrial computer is provided with; Described digit microscope connects described industrial computer, and the control end of described first stepper motor, second stepper motor, light source all connects single-chip microcomputer, and described single-chip microcomputer connects described industrial computer.

Like Fig. 1; First stepper motor 6 of the support fixation elements that comprises digit microscope 1, digit microscope 2 of slide telecontrol equipment described in the present invention and image-taking device, light source 10, platform orbit 4, motion platform 5, the side-to-side movement of drive upper platform; Drive second stepper motor 7 that lower floor's platform seesaws; Vibration damping sheet 8, the second stepper motors connect the connecting link 9 with motion platform.

Like Fig. 2, said motion platform is divided into two-layer up and down.Lower floor is driven by stepper motor, realizes moving forward and backward of motion platform; The upper strata realizes being driven the move left and right of implementation platform by stepper motor.

Like Fig. 3, single-chip microcomputer links to each other with two motors, and controls their motion.Single-chip microcomputer links to each other with LASER Light Source, and controls its open and close.Industrial computer links to each other with single-chip microcomputer, links to each other through Serial Port Line in the present embodiment.Industrial computer is through sending the motion that order comes the controlled motion platform to single-chip microcomputer.Industrial computer links to each other with digit microscope, in the present embodiment, links to each other through the USB line.Industrial computer obtains the milk somatic cell enlarged image through dyeing from digit microscope, identifies the milk somatic cell in the image through the image analysis software analysis on the industrial computer then, and counting, thereby can calculate the milk somatic cell quantity in the unit volume.

The described content of this instructions embodiment only is enumerating the way of realization of inventive concept; Should not being regarded as of protection scope of the present invention only limits to the concrete form that embodiment states, protection scope of the present invention also reach in those skilled in the art conceive according to the present invention the equivalent technologies means that can expect.

Claims

1. based on the milk somatic cell counting assembly of computer vision, it is characterized in that: comprise the slide telecontrol equipment, image-taking device, image analysis apparatus cooperates the light source of described image-taking device; Said slide telecontrol equipment comprises upper strata motion platform, lower floor's motion platform; Described upper platform carries out side-to-side movement by the first stepping driven by motor; Described lower floor platform is driven by second stepper motor and seesaws, and described upper strata motion platform, lower floor's motion platform all are provided with on the orbit; Said image-taking device is made up of digit microscope and support fixation elements thereof; Image analysis apparatus is by industrial computer, the image analysis module that described industrial computer is provided with; Described digit microscope connects described industrial computer, and the control end of described first stepper motor, second stepper motor, light source all connects single-chip microcomputer, and described single-chip microcomputer connects described industrial computer.