CN102789591B - Milk somatic cell counting device based on computer vision - Google Patents

Abstract

The invention discloses a milk somatic cell counting device based on computer vision. The device comprises a slide moving device, an image capture device, an image analyzing device and a light source which is matched with the image capture device, wherein the slide moving device comprises an upper layer moving platform and a lower layer moving platform, the upper layer platform is driven by a first stepping motor to move to the left and right, the lower layer platform is driven by a second stepping motor to move back and forth, and both the upper layer moving platform and the lower layer moving platform are arranged on an orbit; the image capture device comprises a digital microscope and a supporting fixing part of the digital microscope; the image analyzing device comprises an industrial personal computer, and the industrial personal computer is provided with an image analyzing module; and the digital microscope is connected with the industrial personal computer, the first stepping motor, the second stepping motor and the light source are connected with a single chip microcomputer, and the single chip microcomputer is connected with the industrial personal computer. According to the device, the movement of the side is controlled through control of the movement of the moving platforms, during the moving process, amplified images of milk somatic cells are collected and then analyzed, and the quantity of the milk somatic cells is obtained.

Description

Based on the milk somatic cell counting assembly of computer vision

Technical field

The utility model relates to milk somatic cell measuring technique, especially a kind of milk somatic cell method of counting.

Background technology

Body cell in milk is made up of macrophage, lymphocyte, polymorph neutrophile leucocytes and a small amount of breast tissue epithelial cell usually.Somatic number (SCC) refers to the somatic cell count contained in every ml milk.

SCC can reflect the degree of milk milk house by bacteriological infection, can also be used to the damaed cordition estimating milk production of cow.When particularly breast is infected or injures health, body cell can arrive at injury along with blood.Macrophage and core neutrophil engulf bacterium on the one hand, and lymphocyte then controls immune response and produce antibody to destroy with repel bacterial, plays the effect of protection body; Be embodied in somatic quantity in the milk extruded on the other hand obviously to increase.The udder health of somatic number and milk cow is closely related.By somatic detection, the health status of whole cows mammary glandular cell can be weighed.Therefore the mammitis of milk cow is effectively controlled by detecting somatic quantity in raw milk.When SCC is more than 5 × 10 ⁵time, the possibility of milk cow bacterial infection is larger. and the mammitis incidence of disease is very high.Composition transfer in corresponding raw milk increases. the degradation of milk.SCC can be used as the major criterion judging milk quality height: SCC is lower, and the quality of milk is higher; SCC is higher, larger on the impact of milk quality.

Existing detection method mainly contains the sub-mensuration of markon's welfare, fluorescence flow method of counting, electron particles counting method, microscopic method.

SCC can use the sub-mensuration of CMT(markon welfare) measure and obtain.Specific surfactant adds in milk by CMT, when the cell in milk runs into surfactant, can shrink and solidify, and makes cell release nuclifort and aggegation.Cell is more, and state of aggregation is stronger, and the aggegation sheet of appearance is more.CMT method is quick, responsive, and low price, and test method is simple, and equipment needed thereby is few, and reflection result is comparatively accurate.But CMT method is the relative populations of an individual cells, instead of exact magnitude, and think that factor impact is comparatively large, special training personnel should be had fixedly to do this and check.

Also can be used for calculating SCC, i.e. fluorescence flow method of counting by measuring chemiluminescence reaction.This method is in milk, add a kind of fluorescence additive, and this fluorescence additive can by Cell uptake.Then use the illumination of specific wavelength to penetrate milk, now cell can send the fluorescence of another kind of characteristic wavelength.By using an appropriate filter that can identify this characteristic wavelength, the cell number in milk just can be calculated.This method needs, to milk sampling, to need the fluorescence additive choosing appropriate components to mix with it, also needs to select suitable illumination and filtrator.This is that a workload is large and expend higher process.

SCC also can be measured by electron particles counting method.In this, method mainly relies on the change detecting milk electric conductivity, this is because in general, the conductivity in the normal milk of the Conductivity Ratio of mastitis milk is high.Conductivity has the loop of the direct current of probe or interchange to measure by one usually, and this probe is fixed in milk stream.This probe is very sensitive.This probe generally comprises two electrodes, and direct current or alternating current by forming electric current in milk.The change of milk conductivity is measured by the change of electric current in loop.But colloid the precipitation on electrode often can cause the inaccurate of reading in milk.This measuring method also has some shortcomings.This measuring method relies on some changes in milk, and these changes are only had an effect on bacterium and leucocyte and just can be occurred.So it is not too suitable that this initial stage for mastitis is detected.Meanwhile, the repeatability of this method is also poor, because in difference test or different milk cow, and electrolyte ingredient and the great difference of concentration.So use is used alone this method to carry out and diagnoses is risk.

Microscopic method is as the standard method of Somatic Cell Count, and it is generally used for the correctness correcting body cell analyser and additive method.But current microscopic method is only limitted to manual operation, manual operation not only can cause the low of work efficiency, and inevitably produces the error of artificial property.

Summary of the invention

In order to overcome human factor existing for existing milk somatic cell counting assembly, detection efficiency is lower, accuracy is poor deficiency, the utility model provides a kind of and effectively avoids the milk somatic cell counting assembly based on computer vision that human factor affects, detection efficiency is high, accuracy is good.

The utility model solves the technical scheme that its technical matters adopts:

Based on the milk somatic cell counting assembly of computer vision, it is characterized in that: comprise slide telecontrol equipment, image-taking device, image analysis apparatus, the light source of the image-taking device described in cooperation; Described slide telecontrol equipment comprises upper layer motion platform, Lower layer motion platform, described upper platform carries out side-to-side movement by the first stepping driven by motor, described lower floor's platform is seesawed by the second stepping driven by motor, and described upper layer motion platform, Lower layer motion platform are all arranged on orbit; Described image-taking device is made up of digit microscope and support fixation elements thereof; Image analysis apparatus comprises industrial computer, and described industrial computer is provided with image analysis module; Industrial computer described in described digit microscope connects, the control end of the first described stepper motor, the second stepper motor, light source all connects single-chip microcomputer, the industrial computer described in described single-chip microcomputer connects.

This device is by the motion of controlled motion platform thus the motion controlled slide, and the somatic enlarged image of collecting milk in motion process, then carries out analysis to image and draw milk somatic cell quantity.

The utility model has the advantages that: solve conventional microscope method speed slow, and be subject to the shortcoming of manual operation impact, substantially increase accuracy of detection, and can be used for other types cell count, there is wide market outlook and using value.

Accompanying drawing explanation

Fig. 1 is the vertical view of the slide telecontrol equipment described in the utility model and image-taking device.

Fig. 2 is the side view of the slide telecontrol equipment described in the utility model and image-taking device.

Fig. 3 is the annexation figure in the utility model between all parts.

Embodiment

Below in conjunction with accompanying drawing, the utility model is further described.

Based on the milk somatic cell counting assembly of computer vision, comprise slide telecontrol equipment, image-taking device, image analysis apparatus, the light source of the image-taking device described in cooperation; Described slide telecontrol equipment comprises upper layer motion platform, Lower layer motion platform, described upper platform carries out side-to-side movement by the first stepping driven by motor, described lower floor's platform is seesawed by the second stepping driven by motor, and described upper layer motion platform, Lower layer motion platform are all arranged on orbit; Described image-taking device is made up of digit microscope and support fixation elements thereof; Image analysis apparatus comprises industrial computer, and described industrial computer is provided with image analysis module; Industrial computer described in described digit microscope connects, the control end of the first described stepper motor, the second stepper motor, light source all connects single-chip microcomputer, the industrial computer described in described single-chip microcomputer connects.

As Fig. 1, the comprising digit microscope 1, the support fixation elements 2 of digit microscope, light source 10, platform orbit 4, motion platform 5, drive the first stepper motor 6 of upper platform side-to-side movement of slide telecontrol equipment described in the utility model and image-taking device, drive the second stepper motor 7 that lower floor's platform seesaws, vibration damping sheet 8, the second stepper motor connects the connecting link 9 with motion platform.

As Fig. 2, described motion platform is divided into two-layer up and down.Lower floor is driven by stepper motor, realizes the movable of motion platform; Upper strata realizes being driven by stepper motor, moving left and right of implementation platform.

As Fig. 3, single-chip microcomputer is connected with two motors, and controls their motion.Single-chip microcomputer is connected with LASER Light Source, and controls its open and close.Industrial computer is connected with single-chip microcomputer, is connected in the present embodiment by Serial Port Line.Industrial computer is by sending to single-chip microcomputer the motion that order carrys out controlled motion platform.Industrial computer is connected with digit microscope, in the present embodiment, is connected by USB line.Industrial computer obtains through hyperchromatic milk somatic cell enlarged image from digit microscope, is then identified the milk somatic cell in image by the image analysis software analysis on industrial computer, and counts, thus can calculate the milk somatic cell quantity in unit volume.

Content described in this instructions embodiment is only enumerating the way of realization that utility model is conceived; should not being regarded as of protection domain of the present utility model is only limitted to the concrete form that embodiment is stated, protection domain of the present utility model also and conceive the equivalent technologies means that can expect according to the utility model in those skilled in the art.

Claims (1)

1. based on the milk somatic cell counting assembly of computer vision, it is characterized in that: comprise slide telecontrol equipment, image-taking device, image analysis apparatus, the light source of the image-taking device described in cooperation; Described slide telecontrol equipment comprises upper layer motion platform, Lower layer motion platform, described upper platform carries out side-to-side movement by the first stepping driven by motor, described lower floor's platform is seesawed by the second stepping driven by motor, and described upper layer motion platform, Lower layer motion platform are all arranged on orbit; Described image-taking device is made up of digit microscope and support fixation elements thereof; Image analysis apparatus comprises industrial computer, and described industrial computer is provided with image analysis module; Industrial computer described in described digit microscope connects, the control end of the first described stepper motor, the second stepper motor, light source all connects single-chip microcomputer, the industrial computer described in described single-chip microcomputer connects.