CN102783491B - Agricultural bactericide composition containing Flutolanil and difenoconazole and application thereof - Google Patents
Agricultural bactericide composition containing Flutolanil and difenoconazole and application thereof Download PDFInfo
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Abstract
The invention relates to an agricultural bactericide composition containing Flutolanil and difenoconazole. The bactericide composition comprises Flutolanil and difenoconazole as effective ingredients and is prepared by compounding the two ingredients, wherein the weight ratio of Flutolanil to difenoconazole is 1:50-50:1, and the total weight of Flutolanil and difenoconazole in the bactericide composition accounts for 5-60% of the total weight of the bactericide composition. The bactericide composition disclosed herein is suitable for controlling various diseases of cereal crops, especially for controlling rice sheath blight disease, false smut and the like; compared with using single dose, significant synergistic effect is achieved; the application dosage of pesticides is reduced, the pesticide cost is reduced, the generation of pesticide resistance is delayed, and the adverse impact of the pesticide to the environment is reduced.
Description
Technical field
The present invention relates to technical field of pesticide, especially a kind of agriculture bactericide composition that contains flutolanil and Difenoconazole, it can be used for preventing and treating agricultural plant disease.
Background technology
Paddy rice is one of China's staple food crop, and its cultivated area occupy first in the whole nation, account for 1/4 of national arable area.Yet in the whole process of growth of paddy rice, be faced with the threat of numerous pests such as disease, worm, grass, wherein causing harm of rice disease, is having a strong impact on the Rice Production of China.
Banded sclerotial blight is one of disease important in China's Rice Production.This disease can produce a large amount of sclerotium and survive the winter in soil, and Second Year sclerotium adheres to rice strain base portion and completes and just infect, and mycelia constantly spreads formation and infects subsequently, and then the complete stool of causing harm is caused production loss.Due to the height resistance of sclerotium in soil, make this disease become disease the most general in paddy rice producing region.The annual rice sheath blight disease of China is is always prevented and treated area and can be reached more than 300,000,000 mu times, wherein can cause underproduction 10%-30% left and right popular period, when serious, can reach more than 50%.The total losses that rice sheath blight disease causes is far super other diseases of paddy rice often, become the first disease of paddy rice.
One of false smut Yi Shi China Main Rice Diseases, each rice Qu Junyou generation in various degree of Gai Bing China, general time disease tassel yield 4%-6%, can reach more than 50% when serious.This disease not only can make not plump paddy rate, green rice-grain rate, broken rice rate increase, local field underproduction 20%-30%, as long as and the infected seed containing in paddy more than 0.5% can cause person poultry poisoning's symptom.
At present in the middle of the means of prevention for rice sheath blight disease, false smut, chemical control is one of comparatively significant means, comparatively conventional have carbendazim, jinggangmeisu, a triazole type medicament etc., but due to this type of medicament for a long time unicity, continuity, use and make above-mentioned two kinds of germs produce resistance in various degree to the medicament of current use to repeatability, and it is reported, due to this unscientific medication means, the resistance generation time of various medicaments shortens year by year, causes the significantly increase of cost accounting, Environmental costs, medicament R&D costs.Based on this present situation, on existing basis, improve effect of drugs for addressing the above problem comparatively cost-effective means.
Flutolanil (Flutolanil), 3'-isopropoxy-2-(trifluoromethyl) benzanilide, chemical structural formula is:
Flutolanil has in strong inhales conduction and long lasting effect, and it is to having comparatively good preventive effect by imperfect fungus, load are microbial as banded sclerotial blight, smut, damping off etc.Flutolanil is mainly by suppressing mitochondrial respiratory chain compound enzyme II (succinic acid-ubiquinone oxide-reductase enzyme in disease fungus body, succinate-ubiquinone oxidoreductase) activity, thereby make electronics be transferred to ubiquinone from succinic acid, cause thalline ATP normally to synthesize, finally cause thalline because of energy starvation death.
Difenoconazole (Difenoconazole), Difenoconazole, suitable, the chloro-4-[4-methyl-2-1H-1 of trans-3-, 2,4-triazol-1-yl methyl)-1, the mute pentane-2-of 3-bis-yl) phenyl 4-chlorphenyl ether, chemical structural formula is:
Difenoconazole is triazole bactericidal agent, has certain interior suction conduction, for the powdery mildew being caused by sac fungi, imperfect fungus, false smut, leaf spot etc., has comparatively good preventive effect.Be mainly by the activity of Antifungi C14-demethylase (C14-demethylase), sterol in fungus body cannot be normally synthesized, and then cause fungi cannot maintain normal film system and death.
Above two kinds of medicaments started registration used in China except flutolanil from 2008, Difenoconazole rose and has started registration use in the nineties in last century, except Difenoconazole and the composite use of other triazole type more, flutolanil be take single dose registration and is used as main.According to (the Fungicide Resistance Action Committee of bactericide resistance Action Committee, FRAC) relevant report, no matter flutolanil or Difenoconazole, its action site is all more single, and therefore long-term these two kinds of medicaments of single continuous use have higher resistance risk.And the pesticide species of different mechanism is mixed, to use be the comparatively desirable method that delays at present drug resistance problem.The present invention is by a large amount of screenings of formulation of pesticide, to on existing basis, find a kind of can delaying drug resistance, expand the formula of fungicidal spectrum, the remarkable synergistic effect of tool, the composition that final discovery contains flutolanil and Difenoconazole is best group compound, it,, except possessing above-mentioned feature, has comparatively desirable control efficiency for rice sheath blight disease, false smut etc.
At present, not yet find that flutolanil and Difenoconazole are mixed for preventing and treating the relevant report of agricultural plant disease.
Summary of the invention
The object of the invention is easily to produce resistance and fungicidal spectrum in order to overcome the bactericide single dose of above-mentioned two kinds of mechanisms of action, bactericidal effect has the shortcoming of certain limitation and a kind of agriculture bactericide composition that contains flutolanil and Difenoconazole is provided, the two composite use, not only expand germicidal applications scope, delayed the generation of the two resistance simultaneously, improved drug effect, in the situation that guaranteeing optimum control efficiency, reduced the usage amount of medicament, reduce a mu usage amount, thereby reduce costs and reduce environmental hazard.
Technical scheme of the present invention:
An agriculture bactericide composition that contains flutolanil and Difenoconazole, this microbicide compositions be take two yuan that flutolanil and Difenoconazole carry out as active ingredient composite, the weight ratio of flutolanil and Difenoconazole is 1:50～50:1; The percentage that the weight summation of active ingredient flutolanil and Difenoconazole accounts for microbicide compositions gross weight is 5～60%.
In described microbicide compositions except active ingredient, also contain pesticidal preparations auxiliary element, described auxiliary element is auxiliary agent, solvent and/or filler, and wherein auxiliary agent comprises one or more the combination in emulsifier, wetting agent, stabilizing agent, dispersant, antifreezing agent, disintegrant, binding agent, filler and medium.Above material is known substance, is various auxiliary agents conventional in pesticidal preparations, changes to some extent, but be not particularly limited according to being prepared into the different formulations of pesticide.The various fillers that the present invention adds, auxiliary agent, solvent etc. are market sale product and the conventional assistant agent of pesticide producing.
The preferred weight ratio of described flutolanil and Difenoconazole is 1:30～30:1; The percentage that the weight summation of preferred active ingredient flutolanil and Difenoconazole accounts for microbicide compositions gross weight is 10～50%.
Described bactericidal composition can be mixed with according to the method for well known to a person skilled in the art the formulation of applicable agriculture production application such as being prepared as ultra low volume liquids, sustained-release granular formulation, microemulsion, aqueous emulsion, wetting powder, water dispersible granules, suspending agent or oil-suspending agent.
The application of agriculture bactericide composition of the present invention in control cereal crop disease, is particularly useful for preventing and treating rice sheath blight disease, false smut etc.
When application, should there is early stage or the use of disease initial stage of origination in disease in composition pesticide of the present invention.
Composition pesticide of the present invention adopts the method for spraying to use conventionally, also can take other practical techniques according to agriculture needs of production.
Advantage of the present invention:
1, two kinds of active ingredient mechanisms of action in composition pesticide of the present invention are different, the long-term use of single active ingredient has high resistance risk, the two composite use can greatly extend the service life of two kinds of medicaments, for the resistance problem of medicament, manages as a whole and has great importance.
2, composition pesticide of the present invention, empirical tests shows comparatively significant synergistic effect in certain ratio range, the control efficiency of composition has had significant raising than single dose, has also reduced drug cost when having reduced the using dosage of agricultural chemicals, and the adverse effect of environment is also significantly reduced.
3, of the present invention provided pesticide combination has expanded fungicidal spectrum, and prevention and treatment range is more extensive, especially in diseases such as preventing and treating rice sheath blight disease, false smut, has special efficacy.
Accompanying drawing explanation
Fig. 1 is the process chart of the water dispersible granules of preparation agriculture chemicals fungicide composition of the present invention.
Embodiment
Below in conjunction with specific embodiment, the invention will be further described, but be not limitation of the invention.
Embodiment 1:
16% flutolanil Difenoconazole ultra low volume liquids (preparation 1, flutolanil: Difenoconazole=1:15)
Flutolanil 1%, Difenoconazole 15%, OPEO 4%, calcium dodecyl benzene sulfonate 4%, 1-METHYLPYRROLIDONE 12%, methyl-sulfoxide 8%, methyl oleate complement to 100%.Above-mentioned former medicine, solvent, emulsifier are mixed in proportion, stir and obtain 16% flutolanil Difenoconazole ultra low volume liquids.
Embodiment 2:
10% flutolanil Difenoconazole ultra low volume liquids (preparation 2, flutolanil: Difenoconazole=9:1)
Flutolanil 9%, Difenoconazole 1%, OPEO 3%, calcium dodecyl benzene sulfonate 2%, 1-METHYLPYRROLIDONE 12%, methyl-sulfoxide 8%, C10 aromatic hydrocarbons complement to 100%.Above-mentioned former medicine, solvent, emulsifier are mixed in proportion, stir and obtain 10% flutolanil Difenoconazole ultra low volume liquids.
Embodiment 3:
31% flutolanil Difenoconazole sustained-release granular formulation (preparation 3, flutolanil: Difenoconazole=30:1)
Flutolanil 30%, Difenoconazole 1%, polyvinyl alcohol 5%, crosslinked starch 2%, white carbon 3%, polyoxyethylene styryl phenyl ether 2%, water 4%, clay supply 100%.First by crosslinked starch and water Hybrid Heating after 60 minutes, add flutolanil, Difenoconazole, polyoxyethylene styryl phenyl ether, under 50 degrees Celsius, with 2000 revs/min, mix after 5 minutes, under 30 degrees Celsius, solidify 5 hours, subsequent drying is pulverized, and adds clay and polyvinyl alcohol by the granulation of disc granulation device, 60 revs/min of rotating speeds, by grading sieve, obtain the particles needing, make 31% flutolanil Difenoconazole sustained-release granular formulation.
Embodiment 4:
5% flutolanil Difenoconazole sustained-release granular formulation (preparation 4, flutolanil: Difenoconazole=1:4)
Flutolanil 1%, Difenoconazole 4%, polyvinyl alcohol 5%, crosslinked starch 2%, white carbon 3%, polyoxyethylene styryl phenyl ether 2%, water 4%, clay supply 100%.First by crosslinked starch and water Hybrid Heating after 60 minutes, add flutolanil, Difenoconazole, polyoxyethylene styryl phenyl ether, under 50 degrees Celsius, with 2000 revs/min, mix after 5 minutes, under 30 degrees Celsius, solidify 5 hours, subsequent drying is pulverized, and adds clay and polyvinyl alcohol by the granulation of disc granulation device, 60 revs/min of rotating speeds, by grading sieve, obtain the particles needing, make 5% flutolanil Difenoconazole sustained-release granular formulation.
Embodiment 5:
20% flutolanil micro-emulsion of phenyl ether methyl cyclic-azole (preparation 5, flutolanil: Difenoconazole 17:3)
Flutolanil 17%, Difenoconazole 3%, dimethylbenzene 8%, agriculture breast No. 500 8%, agricultural emulsifier NO．600 5%, agriculture breast No. 1,600 5%, sldium lauryl sulfate 2%, ethylene glycol 5%, water complement to 100%.Above-mentioned former medicine, solvent, emulsifier are mixed in proportion, make it be dissolved as even oil phase; Water-soluble component mixes and makes water with water; Under agitation that oil phase is mixed with water, make 20% flutolanil micro-emulsion of phenyl ether methyl cyclic-azole.
Embodiment 6:
31% flutolanil difenoconazole water emulsion (preparation 6, flutolanil: Difenoconazole 1:30)
Flutolanil 1%, Difenoconazole 30%, DMF5%, cyclohexanol 10%, glycerine 5%, phenethyl phenol polyethenoxy ether 10%, calcium dodecyl benzene sulfonate 5%, water complement to 100%.Above-mentioned formula Central Plains medicine, solvent, emulsifiers dissolve are become to even oil phase; Water-soluble component in formula and deionized water mixing are made to water; Under high-speed stirred, oil phase and water are made to 31% flutolanil difenoconazole water emulsion through the homogeneous mixing of high shear.
Embodiment 7:
31% flutolanil difenoconazole water emulsion (preparation 7, flutolanil: Difenoconazole 30:1)
Flutolanil 30%, Difenoconazole 1%, DMF5%, cyclohexanol 10%, glycerine 5%, phenethyl phenol polyethenoxy ether 10%, calcium dodecyl benzene sulfonate 5%, water complement to 100%.Above-mentioned formula Central Plains medicine, solvent, emulsifiers dissolve are become to even oil phase; Water-soluble component in formula and deionized water mixing are made to water; Under high-speed stirred, oil phase and water are made to 31% flutolanil difenoconazole water emulsion through the homogeneous mixing of high shear.
Embodiment 8:
60% flutolanil Difenoconazole wetting powder (preparation 8, flutolanil: Difenoconazole=30:30)
Flutolanil 30%, Difenoconazole 30%, bay alcohol radical sodium sulphate 4%, mersolates 7%, white carbon 8%, kaolin polishing to 100%.Above-mentioned each component in proportion after coarse crushing, is entered in blender and mixed, then after air-flow crushing, make 60% flutolanil Difenoconazole wetting powder.
Embodiment 9:
50% flutolanil Difenoconazole wetting powder (preparation 9, flutolanil: Difenoconazole=25:25)
Flutolanil 25%, Difenoconazole 25%, bay alcohol radical sodium sulphate 4%, mersolates 7%, white carbon 8%, kaolin polishing to 100%.Above-mentioned each component in proportion after coarse crushing, is entered in blender and mixed, then after air-flow crushing, make 50% flutolanil Difenoconazole wetting powder.
Embodiment 10:
31% flutolanil Difenoconazole wetting powder (preparation 10, flutolanil: Difenoconazole=1:30)
Flutolanil 1%, Difenoconazole 30%, pull open powder 4%, polycarboxylate 7%, white carbon 8%, kaolin polishing to 100%.Above-mentioned each component in proportion after coarse crushing, is entered in blender and mixed, then after air-flow crushing, make 31% flutolanil Difenoconazole wetting powder.
Embodiment 11:
31% flutolanil Difenoconazole wetting powder (preparation 11, flutolanil: Difenoconazole=30:1)
Flutolanil 30%, Difenoconazole 1%, spaonin powder 4%, calcium lignosulfonate 7%, white carbon 8%, bentonite polishing to 100%.Above-mentioned each component in proportion after coarse crushing, is entered in blender and mixed, then after air-flow crushing, make 31% flutolanil Difenoconazole wetting powder.
Embodiment 12:
16% flutolanil Difenoconazole wetting powder (preparation 12, flutolanil: Difenoconazole=1:15)
Flutolanil 1%, Difenoconazole 15%, spaonin powder 4%, calcium lignosulfonate 7%, white carbon 8%, bentonite polishing to 100%.Above-mentioned each component in proportion after coarse crushing, is entered in blender and mixed, then after air-flow crushing, make 16% flutolanil Difenoconazole wetting powder.
Embodiment 13:
60% flutolanil difenoconazole water dispersible granule (preparation 13, flutolanil: Difenoconazole=30:30)
Flutolanil 30%, Difenoconazole 30%, neopelex 4%, calcium lignosulfonate 10%, ammonium sulfate 3%, kaolin polishing to 100%.By dry pulverization process, the kneading in proportion of above-mentioned formula one, extruder grain, dry, screening make 60% flutolanil difenoconazole water dispersible granule.
Embodiment 14:
50% flutolanil difenoconazole water dispersible granule (preparation 14, flutolanil: Difenoconazole=25:25)
Flutolanil 25%, Difenoconazole 25%, bay alcohol radical sodium sulphate 4%, mersolates 7%, ammonium sulfate 3%, kaolin polishing to 100%.By dry pulverization process, the kneading in proportion of above-mentioned formula one, extruder grain, dry, screening make 50% flutolanil difenoconazole water dispersible granule.
Embodiment 15:
31% flutolanil difenoconazole water dispersible granule (preparation 15, flutolanil: Difenoconazole=1:30)
Flutolanil 1%, Difenoconazole 30%, pull open powder 4%, polycarboxylate 7%, ammonium sulfate 3%, kaolin polishing to 100%.By dry pulverization process, the kneading in proportion of above-mentioned formula one, extruder grain, dry, screening make 31% flutolanil difenoconazole water dispersible granule.
Embodiment 16:
31% flutolanil difenoconazole water dispersible granule (preparation 16, flutolanil: Difenoconazole=30:1)
Flutolanil 30%, Difenoconazole 1%, spaonin powder 4%, calcium lignosulfonate 7%, ammonium sulfate 3%, bentonite polishing to 100%.By dry pulverization process, the kneading in proportion of above-mentioned formula one, extruder grain, dry, screening make 31% flutolanil difenoconazole water dispersible granule.
Embodiment 17:
20% flutolanil difenoconazole water dispersible granule (preparation 17, flutolanil: Difenoconazole=3:17)
Flutolanil 3%, Difenoconazole 17%, spaonin powder 4%, calcium lignosulfonate 7%, ammonium sulfate 3%, bentonite polishing to 100%.By dry pulverization process, the kneading in proportion of above-mentioned formula one, extruder grain, dry, screening make 20% flutolanil difenoconazole water dispersible granule.
Embodiment 18:
5% agent of flutolanil difenoconazole suspending (preparation 18, flutolanil: Difenoconazole=2:3)
Following material is all weight percentage: flutolanil 2%, and Difenoconazole 3%, fatty alcohol-polyoxyethylene ether 8%, calcium dodecyl benzene sulfonate 12%, diphenylethyllene phenol polyoxyethylene poly-oxygen propylene aether 7%, epoxidized soybean oil 3%, sorbierite 1.5%, surplus is water.Above-mentioned formula is pulverized in proportion in advance, then added in sand mill and grind, after high shear mixing, allotment makes 5% flutolanil difenoconazole suspending agent.
Embodiment 19:
10% agent of flutolanil difenoconazole suspending (preparation 19, flutolanil: Difenoconazole=9:1)
Following material is all weight percentage: flutolanil 9%, and Difenoconazole 1%, fatty alcohol-polyoxyethylene ether 8%, calcium dodecyl benzene sulfonate 12%, diphenylethyllene phenol polyoxyethylene poly-oxygen propylene aether 7%, epoxidized soybean oil 3%, sorbierite 1.5%, surplus is water.Above-mentioned formula is pulverized in proportion in advance, then added in sand mill and grind, after high shear mixing, allotment makes 10% flutolanil difenoconazole suspending agent.
Embodiment 20:
31% agent of flutolanil difenoconazole suspending (preparation 20, flutolanil: Difenoconazole=1:30)
Following material is all weight percentage: flutolanil 1%, and Difenoconazole 30%, fatty alcohol-polyoxyethylene ether 8%, calcium dodecyl benzene sulfonate 10%, diphenylethyllene phenol polyoxyethylene poly-oxygen propylene aether 9%, isopropyl alcohol 1.5%, surplus is water.Above-mentioned formula is pulverized in proportion in advance, then added in sand mill and grind, after high shear mixing, allotment makes 31% flutolanil difenoconazole suspending agent.
Embodiment 21:
11% agent of flutolanil difenoconazole suspending (preparation 21, flutolanil: Difenoconazole=1:10)
Following material is all weight percentage: flutolanil 1%, and Difenoconazole 10%, fatty alcohol-polyoxyethylene ether 8%, calcium dodecyl benzene sulfonate 12%, diphenylethyllene phenol polyoxyethylene poly-oxygen propylene aether 7%, epoxidized soybean oil 3%, sorbierite 1.5%, surplus is water.Above-mentioned formula is pulverized in proportion in advance, then added in sand mill and grind, after high shear mixing, allotment makes 11% flutolanil difenoconazole suspending agent.
Embodiment 22:
31% agent of flutolanil difenoconazole suspending (preparation 22, flutolanil: Difenoconazole=30:1)
Following material is all weight percentage: flutolanil 30%, and Difenoconazole 1%, fatty alcohol-polyoxyethylene ether 8%, calcium dodecyl benzene sulfonate 10%, diphenylethyllene phenol polyoxyethylene poly-oxygen propylene aether 9%, isopropyl alcohol 1.5%, surplus is water.Above-mentioned formula is pulverized in proportion in advance, then added in sand mill and grind, after high shear mixing, allotment makes 31% flutolanil difenoconazole suspending agent.
Embodiment 23:
20% agent of flutolanil difenoconazole suspending (preparation 23, flutolanil: Difenoconazole=15:5)
Following material is all weight percentage: flutolanil 15%, and Difenoconazole 5%, fatty alcohol-polyoxyethylene ether 7.2%, calcium dodecyl benzene sulfonate 12%, diphenylethyllene phenol polyoxyethylene poly-oxygen propylene aether 7%, epoxidized soybean oil 3%, sorbierite 1.5%, surplus is water.Above-mentioned formula is pulverized in proportion in advance, then added in sand mill and grind, after high shear mixing, allotment makes 20% flutolanil difenoconazole suspending agent.
Embodiment 24:
5% flutolanil Difenoconazole oil-suspending agent (preparation 24, flutolanil: Difenoconazole=1:4)
Following material is all weight percentage: flutolanil 1%, and Difenoconazole 4%, the fatty acid modified thing 12% of alkanol, sodium lignin sulfonate 5%, sodium bentonite 6%, surplus is castor oil.After above-mentioned formula is added successively in proportion and grinds and to grind in still, drop into after mixer for well-distribution mixes and make 5% flutolanil Difenoconazole oil-suspending agent.
Embodiment 25:
10% flutolanil Difenoconazole oil-suspending agent (preparation 25, flutolanil: Difenoconazole=6:4)
Following material is all weight percentage: flutolanil 6%, and Difenoconazole 4%, the fatty acid modified thing 12% of alkanol, sodium lignin sulfonate 5%, sodium bentonite 6%, surplus is castor oil.After above-mentioned formula is added successively in proportion and grinds and to grind in still, drop into after mixer for well-distribution mixes and make 10% flutolanil Difenoconazole oil-suspending agent.
Experiment embodiment 1: flutolanil and the test of Difenoconazole composition toxicity test
The definite virulence being mixed to Rhizoctonia solani Kuhn for understanding flutolanil and Difenoconazole combination, the present invention screens for its proportioning.
Rhizoctonia solani Kuhn (separated from surrounding area, Nanning), purifying is cultivated and is verified that by Koch's Postulates it is inoculated in bacterial classification in PDA medium after pathogenic, at 25 ℃, under 90% damp condition, cultivate 7d, mycelia breaks into bacterium cake use with 0.4cm card punch after covering with culture dish.
Adopt mycelial growth rate method to measure flutolanil, Difenoconazole and the impact of the two composition on Rhizoctonia solani Kuhn mycelial growth, setting the two weight proportion is 100:1, 50:1, 30:1, 15:1, 1:1, 1:15, 1:30, 1:50, 1:100 and each single dose be totally 9 kinds of chemicals treatment, each is processed and sets 0.125, 0.25, 0.5, 1, 2, 4, 8, 16, totally 9 concentration gradients of 32mg/L makes PDA flat board, arranging without medicament is contrast, every concentration repeats 3 times, the bacterium cake of accomplishing fluently being inverted in to each under aseptic condition processes after the dull and stereotyped central authorities of PDA, flat board is placed in to 25 ℃, under 90% damp condition, cultivate, (10d left and right) investigation result when mycelial growth diameter reaches culture dish diameter 50% left and right, calculate according to the following equation inhibiting rate, and calculate that by DPS Software of Data Statistics each chemicals treatment is to virulence regression equation, correlation coefficient, EC50 and composite co-toxicity coefficient.
Theoretical toxicity index (the TTI)=∑ (each single dose toxicity index TI * each single dose proportion P) of mixture
Synergy evaluation criterion: co-toxicity coefficient (CTC)≤80 is antagonism, 80 < CTC < 120 are summation action, and CTC >=120 are synergistic effect, and CTC >=150 synergy is very remarkable.
Result of the test is as shown in table 1:
Table 1: the toxicity test of flutolanil proportionings different from Difenoconazole to Rhizoctonia solani Kuhn
When flutolanil and Difenoconazole are in mass ratio between 100:1～1:100 as can be seen from Table 1, two end points are processed CTC value between 80 to 120, present certain drug effect summation action; Proportioning is between 50:1～1:50 time, and each is processed CTC and is all greater than 120, shows that synergistic effect is remarkable, and especially proportioning is within the scope of 30:1～1:30, and each is processed CTC and is all greater than 150, shows that synergistic effect is very remarkable.
Experiment embodiment 2: the field control effectiveness test of flutolanil and Difenoconazole composition for preventing and controlling rice sheath blight disease
Reagent agent, preparation 1-25 collocation method refers to example 1-25, and preparation 26 is 40% flutolanil suspending agent, and preparation 27 is 25% Difenoconazole missible oil, and preparation 28 is 3% jinggangmeisu aqua.
Together with blank, 28 processing are established in test altogether, and each is processed 3 times and repeats, Gong84Ge community, each community randomized arrangement.
In preparation 1-2,2 processing adopt Germany's precious special star (BTX) ultra low volume sprayer evenly to spray according to each processing various dose, within after medicine 7 days, 14 days, carry out investigation result;
In preparation 3-4,4 processing are evenly spread fertilizer over the fields according to each processing various dose, within after medicine 7 days, 14 days, carry out investigation result;
In preparation 5-28,23 processing are by 15L knapsack hand sprayer even spraying for respective concentration.Within after medicine 7 days, 14 days, investigate;
The sick leaf rate of all communities of medicine " Invest, Then Investigate ", by following method, investigate:
According to rice leaf sheath and damage symptom grading, Yi Zhuwei unit, 5 samplings of every community diagonal, every some investigation is connected 5 clumps, every clump of 2 strains, every 10 strain left and right,, record total strain number, diseased plant number and sick progression by totally 25 clumps.
The stage division of the rice sheath blight (take blade as unit):
0 grade: anosis;
1 grade: quaterfoil and following leaf sheath thereof, blade morbidity (take sword-like leave as first leaf);
3 grades: the 3rd blade and following leaf sheath thereof, blade morbidity;
5 grades: the second blade and following leaf sheath thereof, blade morbidity;
7 grades: sword-like leave blade and following leaf sheath thereof, blade morbidity;
9 grades: complete stool morbidity, withered ahead of time.
The data obtained calculates according to following formula:
Table 2: the control efficiency of flutolanil preparation compositions different from Difenoconazole to rice sheath blight disease
By table 2 result of the test, can be found out, after dispenser 7 days, flutolanil and Difenoconazole complex composition all more than 85%, were significantly higher than each single dose contrast medicament and jinggangmeisu contrast to the preventive effect of rice sheath blight disease; After medicine 14 days, composition preventive effect, all more than 90%, was significantly higher than each single dose contrast medicament and jinggangmeisu equally.Test each processing and all do not occur the bad reaction problems such as poisoning.Illustrating thus that flutolanil and Difenoconazole are mixed is used in combination actual control efficiency and is significantly better than single dose, and this result experimental result mentioned with experiment embodiment 1 conforms to, and illustrates that the composite of the two has significant synergies.The composite use of flutolanil and Difenoconazole can reduce dosage, reduces drug cost, meanwhile can delay the generation of medicament resistance problem, and agricultural synthesis is administered and had great significance.
Claims (7)
1. an agriculture bactericide composition that contains flutolanil and Difenoconazole, it is characterized in that: this microbicide compositions be take two yuan that flutolanil and Difenoconazole carry out as active ingredient composite, the weight ratio of flutolanil and Difenoconazole is 1:15～1:50,50:1 ~ 1: 1.
2. the agriculture bactericide composition that contains flutolanil and Difenoconazole according to claim 1, is characterized in that: the weight ratio of flutolanil and Difenoconazole is 1:15～1:30,30:1～1: 1.
3. the agriculture bactericide composition that contains flutolanil and Difenoconazole according to claim 1 and 2, it is characterized in that: in described microbicide compositions, also contain pesticidal preparations auxiliary element, described auxiliary element is solvent, filler or auxiliary agent, and wherein auxiliary agent comprises one or more the combination in emulsifier, wetting agent, stabilizing agent, dispersant, antifreezing agent, disintegrant, binding agent, filler and medium.
4. the agriculture bactericide composition that contains flutolanil and Difenoconazole according to claim 3, is characterized in that: in described microbicide compositions, to account for the percentage of microbicide compositions gross weight be 5～60% to the weight summation of active ingredient flutolanil and Difenoconazole.
5. the agriculture bactericide composition that contains flutolanil and Difenoconazole according to claim 3, is characterized in that: in described microbicide compositions, to account for the percentage of microbicide compositions gross weight be 10～50% to the weight summation of active ingredient flutolanil and Difenoconazole.
6. according to the agriculture bactericide composition that contains flutolanil and Difenoconazole described in claim 4 or 5, it is characterized in that: the be prepared as ultra low volume liquids of described bactericidal composition, sustained-release granular formulation, microemulsion, aqueous emulsion, wetting powder, water dispersible granules, suspending agent or oil-suspending agent.
7. the agriculture bactericide composition that contains flutolanil and Difenoconazole according to claim 1, is characterized in that: the application of described microbicide compositions in control cereal crop disease.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201210242982.6A CN102783491B (en) | 2012-07-13 | 2012-07-13 | Agricultural bactericide composition containing Flutolanil and difenoconazole and application thereof |
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