CN102783491A - Agricultural bactericide composition containing Flutolanil and difenoconazole and application thereof - Google Patents
Agricultural bactericide composition containing Flutolanil and difenoconazole and application thereof Download PDFInfo
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Abstract
The invention relates to an agricultural bactericide composition containing Flutolanil and difenoconazole. The bactericide composition comprises Flutolanil and difenoconazole as effective ingredients and is prepared by compounding the two ingredients, wherein the weight ratio of Flutolanil to difenoconazole is 1:50-50:1, and the total weight of Flutolanil and difenoconazole in the bactericide composition accounts for 5-60% of the total weight of the bactericide composition. The bactericide composition disclosed herein is suitable for controlling various diseases of cereal crops, especially for controlling rice sheath blight disease, false smut and the like; compared with using single dose, significant synergistic effect is achieved; the application dosage of pesticides is reduced, the pesticide cost is reduced, the generation of pesticide resistance is delayed, and the adverse impact of the pesticide to the environment is reduced.
Description
Technical field
The present invention relates to technical field of pesticide, especially a kind of agriculture bactericide composition that contains flutolanil and Difenoconazole, it can be used for preventing and treating the agricultural plant disease.
Background technology
Paddy rice is one of China's staple food crop, and its cultivated area occupy first in the whole nation, account for 1/4 of national arable area.Yet in the whole growth process of paddy rice, be faced with numerous threats such as pests such as disease, worm, grass, wherein causing harm of rice disease, having a strong impact on the Rice Production of China.
Banded sclerotial blight is one of disease important in China's Rice Production.This disease can produce a large amount of sclerotium and in soil, survive the winter, and 1 year sclerotium adheres to rice strain base portion and accomplish and just infect, and mycelia constantly spreads formation and infects subsequently, and then the complete stool of causing harm is caused production loss.Because the height resistance of sclerotium in soil makes this disease become disease the most general in the paddy rice producing region.The annual rice sheath blight disease of China is is always prevented and treated area and can be reached more than 300,000,000 mu times, and can cause about underproduction 10%-30% wherein popular period, can reach more than 50% when serious.The total losses that rice sheath blight disease caused often far surpasses other diseases of paddy rice, becomes the first disease of paddy rice.
False smut also is one of main disease of China paddy rice, and this disease is in each rice Qu Junyou generation in various degree of China, and general time disease tassel yield 4%-6% can reach more than 50% when serious.This disease not only can make not plump paddy rate, green rice-grain rate, broken rice rate increase, local field piece underproduction 20%-30%, as long as and the infected seed that contains in the paddy more than 0.5% can cause person poultry poisoning's symptom.
At present in the middle of the means of prevention to rice sheath blight disease, false smut; Chemical control is one of comparatively significant means; Comparatively commonly used have carbendazim, jinggangmeisu, a triazole type medicament etc.; But because unicity, continuity, the use of repeatability ground for a long time of this type of medicament made above-mentioned two kinds of germs that the medicament of current use is produced resistance in various degree, and it is reported, because this unscientific medication means; The resistance generation time of various medicaments shortens year by year, causes the significantly increase of control cost, Environmental costs, medicament R&D costs.Based on this present situation, on existing basis, improve effect of drugs for addressing the above problem comparatively cost-effective means.
Flutolanil (Flutolanil), 3'-isopropoxy-2-(trifluoromethyl) benzanilide, chemical structural formula is:
Flutolanil has inhales conduction and long lasting effect in strong, its to by imperfect fungus, load is microbial has comparatively good preventive effect like banded sclerotial blight, smut, damping off etc.Flutolanil is mainly through suppressing mitochondrial respiratory chain compound enzyme II (succinic acid-ubiquinone oxide-reductase enzyme in the disease fungus body; Succinate-ubiquinone oxidoreductase) activity; Thereby make electronics be transferred to ubiquinone from succinic acid; Cause thalline ATP normally to synthesize, finally cause thalline dead because of energy starvation.
Difenoconazole (Difenoconazole), Difenoconazole, suitable, anti--3-chloro-4-[4-methyl-2-1H-1,2,4-triazol-1-yl methyl)-1,3-two mute pentane-2-yls) phenyl 4-chlorphenyl ether, chemical structural formula is:
Difenoconazole is a triazole bactericidal agent, has certain interior suction conduction, has comparatively good preventive effect for the powdery mildew that is caused by sac fungi, imperfect fungus, false smut, leaf spot etc.Mainly be through suppressing the activity of fungi C14-demethylase (C14-demethylase), the interior sterol of fungus body can't normally being synthesized, and then cause fungi can't keep normal film system and death.
More than two kinds of medicaments begin registration uses in China from 2008 except that flutolanil; Difenoconazole rises in the nineties in last century and has promptly begun the registration use; Except that Difenoconazole and the composite use of other triazole type were more, flutolanil was used for the master with single agent registration.According to (the Fungicide Resistance Action Committee of bactericide resistance Action Committee; FRAC) relevant report; No matter flutolanil, still Difenoconazole, its action site is all more single, and therefore long-term these two kinds of medicaments of single continuous use have higher resistance risk.To use be the comparatively desirable method that delays the drug resistance problem at present and the pesticide species of different mechanism is mixed.The present invention is through a large amount of screenings of formulation of pesticide; In the hope of find on existing basis a kind of can delaying drug resistance, enlarge the prescription of fungicidal spectrum, the remarkable synergistic effect of tool; The composition that final discovery contains flutolanil and Difenoconazole is the best group compound; It has comparatively desirable control efficiency for rice sheath blight disease, false smut etc. except that possessing above-mentioned characteristic.
At present, not finding as yet that flutolanil and Difenoconazole are mixed is used to prevent and treat the relevant report of agricultural plant disease.
Summary of the invention
The objective of the invention is for the bactericide list agent that overcomes above-mentioned two kinds of mechanisms of action is prone to produce resistance and fungicidal spectrum, bactericidal effect has certain circumscribed shortcoming and a kind of agriculture bactericide composition that contains flutolanil and Difenoconazole is provided; The two composite use; Not only enlarge the germicidal applications scope; Delay the generation of the two resistance simultaneously, improved drug effect, under the situation that guarantees optimum control efficiency, reduced the usage amount of medicament; Reduce a mu usage amount, thereby reduce cost and reduce environmental hazard.
Technical scheme of the present invention:
A kind of agriculture bactericide composition that contains flutolanil and Difenoconazole, this microbicide compositions are to be that two yuan of carrying out of active ingredient are composite with flutolanil and Difenoconazole, and the weight ratio of flutolanil and Difenoconazole is 1:50～50:1; The percentage that the weight summation of active ingredient flutolanil and Difenoconazole accounts for the microbicide compositions gross weight is 5～60%.
In the said microbicide compositions except active ingredient; Also contain the pesticidal preparations auxiliary element; Said auxiliary element is auxiliary agent, solvent and/or filler, wherein comprises one or more the combination in emulsifier, wetting agent, stabilizing agent, dispersant, antifreezing agent, disintegrant, binding agent, filler and the medium in the auxiliary agent.Above material is known substance, is various auxiliary agents commonly used in the pesticidal preparations, changes to some extent according to being prepared into the different formulations of pesticide, but does not have special qualification.The various fillers that the present invention added, auxiliary agent, solvent etc. are market sale product and pesticide producing assistant agent commonly used.
The preferred weight ratio of said flutolanil and Difenoconazole is 1:30～30:1; The percentage that the weight summation of preferred active ingredient flutolanil and Difenoconazole accounts for the microbicide compositions gross weight is 10～50%.
Said bactericidal composition can be mixed with the formulation of suitable agriculture production application such as being prepared as ultra low volume liquids, sustained-release granular formulation, microemulsion, aqueous emulsion, wetting powder, water dispersible granules, suspending agent or oil-suspending agent according to the method for well known to a person skilled in the art.
The application of agriculture bactericide composition of the present invention on control cereal crop disease is particularly useful for preventing and treating rice sheath blight disease, false smut etc.
Early stage or the use of disease initial stage of origination should take place in disease in composition pesticide of the present invention when using.
Composition pesticide of the present invention adopts the method for spraying to use usually, also can take other practical techniques according to agriculture needs of production.
Advantage of the present invention:
1, two kinds of active ingredient mechanisms of action in the composition pesticide of the present invention are different; The long-term use of single active ingredient has high resistance risk; The two composite use can greatly prolong the service life of two kinds of medicaments, manages as a whole for the resistance problem of medicament to have great importance.
2, composition pesticide of the present invention; Empirical tests shows comparatively significant synergistic effect in certain ratio range; The control efficiency of composition has had significant raising than single agent, has also reduced drug cost when having reduced the using dosage of agricultural chemicals, and the adverse effect of environment is also significantly reduced.
3, the pesticide combination that provides of the present invention has enlarged fungicidal spectrum, and prevention and treatment range is more extensive, especially has special efficacy in diseases such as preventing and treating rice sheath blight disease, false smut.
Description of drawings
Fig. 1 is the process chart of the water dispersible granules of preparation agriculture chemicals fungicide composition of the present invention.
Embodiment
Below in conjunction with specific embodiment the present invention is described further, but is not qualification of the present invention.
Embodiment 1:
16% flutolanil Difenoconazole ultra low volume liquids (preparation 1, flutolanil: Difenoconazole=1:15)
Flutolanil 1%, Difenoconazole 15%, OPEO 4%, calcium dodecyl benzene sulfonate 4%, N-Methyl pyrrolidone 12%, methyl-sulfoxide 8%, methyl oleate complement to 100%.Above-mentioned former medicine, solvent, emulsifier are mixed in proportion, stir and promptly get 16% flutolanil Difenoconazole ultra low volume liquids.
Embodiment 2:
10% flutolanil Difenoconazole ultra low volume liquids (preparation 2, flutolanil: Difenoconazole=9:1)
Flutolanil 9%, Difenoconazole 1%, OPEO 3%, calcium dodecyl benzene sulfonate 2%, N-Methyl pyrrolidone 12%, methyl-sulfoxide 8%, C10 aromatic hydrocarbons complement to 100%.Above-mentioned former medicine, solvent, emulsifier are mixed in proportion, stir and promptly get 10% flutolanil Difenoconazole ultra low volume liquids.
Embodiment 3:
31% flutolanil Difenoconazole sustained-release granular formulation (preparation 3, flutolanil: Difenoconazole=30:1)
Flutolanil 30%, Difenoconazole 1%, polyvinyl alcohol 5%, crosslinked starch 2%, white carbon 3%, polyoxyethylene styryl phenyl ether 2%, water 4%, clay supply 100%.Earlier crosslinked starch and water Hybrid Heating after 60 minutes, are added flutolanil, Difenoconazole, polyoxyethylene styryl phenyl ether, under 50 degrees centigrade with 2000 rev/mins of mixing after 5 minutes; Under 30 degrees centigrade, solidified 5 hours; Subsequent drying is pulverized, and adds clay and polyvinyl alcohol through the granulation of disk nodulizer, 60 rev/mins of rotating speeds; Obtain the particle diameter particle that needs through grading sieve, promptly make 31% flutolanil Difenoconazole sustained-release granular formulation.
Embodiment 4:
5% flutolanil Difenoconazole sustained-release granular formulation (preparation 4, flutolanil: Difenoconazole=1:4)
Flutolanil 1%, Difenoconazole 4%, polyvinyl alcohol 5%, crosslinked starch 2%, white carbon 3%, polyoxyethylene styryl phenyl ether 2%, water 4%, clay supply 100%.Earlier crosslinked starch and water Hybrid Heating after 60 minutes, are added flutolanil, Difenoconazole, polyoxyethylene styryl phenyl ether, under 50 degrees centigrade with 2000 rev/mins of mixing after 5 minutes; Under 30 degrees centigrade, solidified 5 hours; Subsequent drying is pulverized, and adds clay and polyvinyl alcohol through the granulation of disk nodulizer, 60 rev/mins of rotating speeds; Obtain the particle diameter particle that needs through grading sieve, promptly make 5% flutolanil Difenoconazole sustained-release granular formulation.
Embodiment 5:
20% flutolanil micro-emulsion of phenyl ether methyl cyclic-azole (preparation 5, flutolanil: Difenoconazole 17:3)
Flutolanil 17%, Difenoconazole 3%, xylol 8%, farming breast No. 500 8%, agricultural emulsifier NO 5%, farming breast No. 1,600 5%, sldium lauryl sulfate 2%, ethylene glycol 5%, water complement to 100%.Above-mentioned former medicine, solvent, emulsifier are mixed in proportion, make it be dissolved as even oil phase; The mixed water that gets of water-soluble component and water; Under agitation that oil phase is mixed with water, promptly make 20% flutolanil micro-emulsion of phenyl ether methyl cyclic-azole.
Embodiment 6:
31% flutolanil Difenoconazole aqueous emulsion (preparation 6, flutolanil: Difenoconazole 1:30)
Flutolanil 1%, Difenoconazole 30%, DMF5%, cyclohexanol 10%, glycerine 5%, phenethyl phenol polyethenoxy ether 10%, calcium dodecyl benzene sulfonate 5%, water complement to 100%.Above-mentioned prescription Central Plains medicine, solvent, emulsifiers dissolve are become even oil phase; The mixed water that gets of water-soluble component and deionized water in will filling a prescription; Under high-speed stirred, oil phase and water are spared the mixed 31% flutolanil Difenoconazole aqueous emulsion that gets of matter through high shear.
Embodiment 7:
31% flutolanil Difenoconazole aqueous emulsion (preparation 7, flutolanil: Difenoconazole 30:1)
Flutolanil 30%, Difenoconazole 1%, DMF5%, cyclohexanol 10%, glycerine 5%, phenethyl phenol polyethenoxy ether 10%, calcium dodecyl benzene sulfonate 5%, water complement to 100%.Above-mentioned prescription Central Plains medicine, solvent, emulsifiers dissolve are become even oil phase; The mixed water that gets of water-soluble component and deionized water in will filling a prescription; Under high-speed stirred, oil phase and water are spared the mixed 31% flutolanil Difenoconazole aqueous emulsion that gets of matter through high shear.
Embodiment 8:
60% flutolanil Difenoconazole wetting powder (preparation 8, flutolanil: Difenoconazole=30:30)
Flutolanil 30%, Difenoconazole 30%, bay alcohol radical sodium sulphate 4%, mersolates 7%, white carbon 8%, kaolin polishing to 100%.With above-mentioned each component in proportion after the coarse crushing, get in the blender and mix, behind comminution by gas stream, promptly make 60% flutolanil Difenoconazole wetting powder again.
Embodiment 9:
50% flutolanil Difenoconazole wetting powder (preparation 9, flutolanil: Difenoconazole=25:25)
Flutolanil 25%, Difenoconazole 25%, bay alcohol radical sodium sulphate 4%, mersolates 7%, white carbon 8%, kaolin polishing to 100%.With above-mentioned each component in proportion after the coarse crushing, get in the blender and mix, behind comminution by gas stream, promptly make 50% flutolanil Difenoconazole wetting powder again.
Embodiment 10:
31% flutolanil Difenoconazole wetting powder (preparation 10, flutolanil: Difenoconazole=1:30)
Flutolanil 1%, Difenoconazole 30%, draw back powder 4%, polycarboxylate 7%, white carbon 8%, kaolin polishing to 100%.With above-mentioned each component in proportion after the coarse crushing, get in the blender and mix, behind comminution by gas stream, promptly make 31% flutolanil Difenoconazole wetting powder again.
Embodiment 11:
31% flutolanil Difenoconazole wetting powder (preparation 11, flutolanil: Difenoconazole=30:1)
Flutolanil 30%, Difenoconazole 1%, spaonin powder 4%, calcium lignosulfonate 7%, white carbon 8%, bentonite polishing to 100%.With above-mentioned each component in proportion after the coarse crushing, get in the blender and mix, behind comminution by gas stream, promptly make 31% flutolanil Difenoconazole wetting powder again.
Embodiment 12:
16% flutolanil Difenoconazole wetting powder (preparation 12, flutolanil: Difenoconazole=1:15)
Flutolanil 1%, Difenoconazole 15%, spaonin powder 4%, calcium lignosulfonate 7%, white carbon 8%, bentonite polishing to 100%.With above-mentioned each component in proportion after the coarse crushing, get in the blender and mix, behind comminution by gas stream, promptly make 16% flutolanil Difenoconazole wetting powder again.
Embodiment 13:
60% flutolanil difenoconazole water dispersible granule (preparation 13, flutolanil: Difenoconazole=30:30)
Flutolanil 30%, Difenoconazole 30%, neopelex 4%, calcium lignosulfonate 10%, ammonium sulfate 3%, kaolin polishing to 100%.With dry pulverization process, the kneading in proportion of above-mentioned prescription one, extruder grain, drying, screening make 60% flutolanil difenoconazole water dispersible granule.
Embodiment 14:
50% flutolanil difenoconazole water dispersible granule (preparation 14, flutolanil: Difenoconazole=25:25)
Flutolanil 25%, Difenoconazole 25%, bay alcohol radical sodium sulphate 4%, mersolates 7%, ammonium sulfate 3%, kaolin polishing to 100%.With dry pulverization process, the kneading in proportion of above-mentioned prescription one, extruder grain, drying, screening make 50% flutolanil difenoconazole water dispersible granule.
Embodiment 15:
31% flutolanil difenoconazole water dispersible granule (preparation 15, flutolanil: Difenoconazole=1:30)
Flutolanil 1%, Difenoconazole 30%, draw back powder 4%, polycarboxylate 7%, ammonium sulfate 3%, kaolin polishing to 100%.With dry pulverization process, the kneading in proportion of above-mentioned prescription one, extruder grain, drying, screening make 31% flutolanil difenoconazole water dispersible granule.
Embodiment 16:
31% flutolanil difenoconazole water dispersible granule (preparation 16, flutolanil: Difenoconazole=30:1)
Flutolanil 30%, Difenoconazole 1%, spaonin powder 4%, calcium lignosulfonate 7%, ammonium sulfate 3%, bentonite polishing to 100%.With dry pulverization process, the kneading in proportion of above-mentioned prescription one, extruder grain, drying, screening make 31% flutolanil difenoconazole water dispersible granule.
Embodiment 17:
20% flutolanil difenoconazole water dispersible granule (preparation 17, flutolanil: Difenoconazole=3:17)
Flutolanil 3%, Difenoconazole 17%, spaonin powder 4%, calcium lignosulfonate 7%, ammonium sulfate 3%, bentonite polishing to 100%.With dry pulverization process, the kneading in proportion of above-mentioned prescription one, extruder grain, drying, screening make 20% flutolanil difenoconazole water dispersible granule.
Embodiment 18:
5% flutolanil difenoconazole suspending agent (preparation 18, flutolanil: Difenoconazole=2:3)
Following material all is weight percentage: flutolanil 2%, and Difenoconazole 3%, AEO 8%, calcium dodecyl benzene sulfonate 12%, diphenylethyllene phenol polyoxyethylene poly-oxygen propylene aether 7%, epoxidized soybean oil 3%, sorbierite 1.5%, surplus is a water.Above-mentioned prescription is pulverized in proportion in advance, added in the sand mill and grind, allotment makes 5% flutolanil difenoconazole suspending agent after high shear mixing.
Embodiment 19:
10% flutolanil difenoconazole suspending agent (preparation 19, flutolanil: Difenoconazole=9:1)
Following material all is weight percentage: flutolanil 9%, and Difenoconazole 1%, AEO 8%, calcium dodecyl benzene sulfonate 12%, diphenylethyllene phenol polyoxyethylene poly-oxygen propylene aether 7%, epoxidized soybean oil 3%, sorbierite 1.5%, surplus is a water.Above-mentioned prescription is pulverized in proportion in advance, added in the sand mill and grind, allotment makes 10% flutolanil difenoconazole suspending agent after high shear mixing.
Embodiment 20:
31% flutolanil difenoconazole suspending agent (preparation 20, flutolanil: Difenoconazole=1:30)
Following material all is weight percentage: flutolanil 1%, and Difenoconazole 30%, AEO 8%, calcium dodecyl benzene sulfonate 10%, diphenylethyllene phenol polyoxyethylene poly-oxygen propylene aether 9%, isopropyl alcohol 1.5%, surplus is a water.Above-mentioned prescription is pulverized in proportion in advance, added in the sand mill and grind, allotment makes 31% flutolanil difenoconazole suspending agent after high shear mixing.
Embodiment 21:
11% flutolanil difenoconazole suspending agent (preparation 21, flutolanil: Difenoconazole=1:10)
Following material all is weight percentage: flutolanil 1%, and Difenoconazole 10%, AEO 8%, calcium dodecyl benzene sulfonate 12%, diphenylethyllene phenol polyoxyethylene poly-oxygen propylene aether 7%, epoxidized soybean oil 3%, sorbierite 1.5%, surplus is a water.Above-mentioned prescription is pulverized in proportion in advance, added in the sand mill and grind, allotment makes 11% flutolanil difenoconazole suspending agent after high shear mixing.
Embodiment 22:
31% flutolanil difenoconazole suspending agent (preparation 22, flutolanil: Difenoconazole=30:1)
Following material all is weight percentage: flutolanil 30%, and Difenoconazole 1%, AEO 8%, calcium dodecyl benzene sulfonate 10%, diphenylethyllene phenol polyoxyethylene poly-oxygen propylene aether 9%, isopropyl alcohol 1.5%, surplus is a water.Above-mentioned prescription is pulverized in proportion in advance, added in the sand mill and grind, allotment makes 31% flutolanil difenoconazole suspending agent after high shear mixing.
Embodiment 23:
20% flutolanil difenoconazole suspending agent (preparation 23, flutolanil: Difenoconazole=15:5)
Following material all is weight percentage: flutolanil 15%, and Difenoconazole 5%, AEO 7.2%, calcium dodecyl benzene sulfonate 12%, diphenylethyllene phenol polyoxyethylene poly-oxygen propylene aether 7%, epoxidized soybean oil 3%, sorbierite 1.5%, surplus is a water.Above-mentioned prescription is pulverized in proportion in advance, added in the sand mill and grind, allotment makes 20% flutolanil difenoconazole suspending agent after high shear mixing.
Embodiment 24:
5% flutolanil Difenoconazole oil-suspending agent (preparation 24, flutolanil: Difenoconazole=1:4)
Following material all is weight percentage: flutolanil 1%, and Difenoconazole 4%, the fatty acid modified thing 12% of alkanol, sodium lignin sulfonate 5%, sodium bentonite 6%, surplus is a castor oil.With above-mentioned prescription add to grind successively in proportion grind in the still after, promptly make 5% flutolanil Difenoconazole oil-suspending agent after dropping into homogeneous blender mixing.
Embodiment 25:
10% flutolanil Difenoconazole oil-suspending agent (preparation 25, flutolanil: Difenoconazole=6:4)
Following material all is weight percentage: flutolanil 6%, and Difenoconazole 4%, the fatty acid modified thing 12% of alkanol, sodium lignin sulfonate 5%, sodium bentonite 6%, surplus is a castor oil.With above-mentioned prescription add to grind successively in proportion grind in the still after, promptly make 10% flutolanil Difenoconazole oil-suspending agent after dropping into homogeneous blender mixing.
Experiment embodiment 1: flutolanil and the test of Difenoconazole composition toxicity test
Be mixed to the definite virulence of Rhizoctonia solani Kuhn for understanding the combination of flutolanil and Difenoconazole, the present invention screens for its proportioning.
Rhizoctonia solani Kuhn (separation) from the surrounding area, Nanning; Purifying cultivate after the Koch's Postulates checking its pathogenic after with bacterial classification inoculation in the PDA medium; At 25 ℃, 90% damp condition is cultivated 7d down, and mycelia breaks into bacterium cake usefulness with the 0.4cm card punch after covering with culture dish.
Adopt the mycelial growth rate method to measure the influence of flutolanil, Difenoconazole and the two composition to the Rhizoctonia solani Kuhn mycelial growth; Setting the two weight proportion is 100:1,50:1,30:1,15:1,1:1,1:15,1:30,1:50,1:100 and each single agent totally 9 kinds of chemicals treatment; Each processing setting 0.125,0.25,0.5,1,2,4,8,16,32mg/L totally 9 concentration gradients process the PDA flat board; No medicament is set is contrast; Every concentration repeats 3 times, under aseptic condition, the bacterium cake of accomplishing fluently is inverted in after each handles the dull and stereotyped central authorities of PDA, and flat board is placed 25 ℃; 90% damp condition is cultivated down; (about 10d) investigation result when treating that the mycelial growth diameter reaches culture dish diameter 50% left and right sides is calculated inhibiting rate according to the following equation, and calculates each chemicals treatment to virulence regression equation, correlation coefficient, EC50 and composite co-toxicity coefficient by DPS data statistics software.
The theoretical toxicity index (TTI) of mixture=∑ (each single agent toxicity index TI * each single agent proportion P)
The synergy evaluation criterion: co-toxicity coefficient (CTC)≤80 is an antagonism, and 80＜CTC＜120 are summation action, and CTC>=120 are synergistic effect, and CTC>=150 synergy are very remarkable.
Result of the test is as shown in table 1:
Table 1: the different proportionings with Difenoconazole of flutolanil are to the toxicity test of Rhizoctonia solani Kuhn
Can find out flutolanil and Difenoconazole by mass ratio between 100:1～1:100 the time by table 1, two end points are handled the CTC values between 80 to 120, present certain drug effect summation action; Proportioning is between 50:1～1:50 the time, and each handles CTC all greater than 120, shows that synergistic effect is remarkable, and especially proportioning is in 30:1～1:30 scope, and each handles CTC all greater than 150, shows that synergistic effect is very remarkable.
Experiment embodiment 2: the field control effectiveness test of flutolanil and Difenoconazole composition for preventing and controlling rice sheath blight disease
Supply the reagent agent, preparation 1-25 collocation method sees instance 1-25 for details, and preparation 26 is 40% flutolanil suspending agent, and preparation 27 is 25% Difenoconazole missible oil, and preparation 28 is 3% jinggangmeisu aqua.
Together with blank, 28 processing are established in test altogether, and each is handled 3 times and repeats, totally 84 sub-districts, each sub-district randomized arrangement.
2 handle to be adopted Germany's precious special star (BTX) ultra low volume sprayers to handle various dose evenly to spray according to each among the preparation 1-2, carry out investigation result behind the medicine in 7 days, 14 days;
4 processing are handled various dose and are evenly spread fertilizer over the fields according to each among the preparation 3-4, carry out investigation result behind the medicine in 7 days, 14 days;
23 processing are pressed respective concentration with 15L knapsack hand sprayer even spraying among the preparation 5-28.Investigated in 7 days, 14 days behind the medicine;
The sick leaf rate of all sub-districts of medicine " Invest, Then Investigate ", investigate by following method:
According to rice leaf sheath and damage symptom grading, be unit with the strain, 5 samplings of every sub-district diagonal, every some investigation links to each other 5 clumps, and every clump 2 strain about every 10 strain, totally 25 clumps, is write down total strain number, diseased plant number and sick progression.
The stage division of the rice sheath blight (is unit with the blade):
0 grade: anosis;
1 grade: quaterfoil and following each leaf sheath thereof, blade morbidity (is first leaf with sword-like leave);
3 grades: the 3rd blade and following each leaf sheath thereof, blade morbidity;
5 grades: second blade and following each leaf sheath thereof, blade morbidity;
7 grades: sword-like leave blade and following each leaf sheath thereof, blade morbidity;
9 grades: the complete stool morbidity, withered ahead of time.
The gained data are calculated according to formula:
Table 2: the different preparation compsns with Difenoconazole of flutolanil are to the control efficiency of rice sheath blight disease
Can find out that by table 2 result of the test after the dispenser 7 days, flutolanil and Difenoconazole complex composition all more than 85%, were significantly higher than each single agent contrast medicament and jinggangmeisu contrast to the preventive effect of rice sheath blight disease; Behind the medicine 14 days, the composition preventive effect was significantly higher than each single agent contrast medicament and jinggangmeisu equally all more than 90%.Test each and handle bad reaction problems such as poisoning all do not occur.Explain that thus the combination that is mixed of flutolanil and Difenoconazole uses actual control efficiency significantly to be superior to single agent, this result conforms to experiment embodiment 1 mentioned experimental result, explains that the composite of the two has significant synergies.The composite use of flutolanil and Difenoconazole can reduce dosage, reduces drug cost, meanwhile can delay the generation of medicament resistance problem, agricultural synthesis is administered have great significance.
Claims (7)
1. agriculture bactericide composition that contains flutolanil and Difenoconazole; It is characterized in that: this microbicide compositions is to be that two yuan of carrying out of active ingredient are composite with flutolanil and Difenoconazole, and the weight ratio of flutolanil and Difenoconazole is 1:50～50:1.
2. the agriculture bactericide composition that contains flutolanil and Difenoconazole according to claim 1 is characterized in that: the weight ratio of flutolanil and Difenoconazole is 1:30～30:1.
3. the agriculture bactericide composition that contains flutolanil and Difenoconazole according to claim 1 and 2; It is characterized in that: also contain the pesticidal preparations auxiliary element in the said microbicide compositions; Said auxiliary element is auxiliary agent, solvent and/or filler, wherein comprises one or more the combination in emulsifier, wetting agent, stabilizing agent, dispersant, antifreezing agent, disintegrant, binding agent, filler and the medium in the auxiliary agent.
4. the agriculture bactericide composition that contains flutolanil and Difenoconazole according to claim 3 is characterized in that: to account for the percentage of microbicide compositions gross weight be 5～60% to the weight summation of active ingredient flutolanil and Difenoconazole in the said microbicide compositions.
5. the agriculture bactericide composition that contains flutolanil and Difenoconazole according to claim 3 is characterized in that: to account for the percentage of microbicide compositions gross weight be 10～50% to the weight summation of active ingredient flutolanil and Difenoconazole in the said microbicide compositions.
6. according to claim 4 or the 5 described agriculture bactericide compositions that contain flutolanil and Difenoconazole, it is characterized in that: the be prepared as ultra low volume liquids of said bactericidal composition, sustained-release granular formulation, microemulsion, aqueous emulsion, wetting powder, water dispersible granules, suspending agent or oil-suspending agent.
7. according to the arbitrary described agriculture bactericide composition that contains flutolanil and Difenoconazole of claim 1 to 6, it is characterized in that: said microbicide compositions is being prevented and treated application, the especially banded sclerotial blight on the cereal crop disease, the control of false smut.
Priority Applications (1)
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| CN201210242982.6A CN102783491B (en) | 2012-07-13 | 2012-07-13 | Agricultural bactericide composition containing Flutolanil and difenoconazole and application thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103073349A (en) * | 2012-12-29 | 2013-05-01 | 广东中迅农科股份有限公司 | Drug fertilizer granules for preventing rice damping-off and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101637162A (en) * | 2009-09-08 | 2010-02-03 | 深圳诺普信农化股份有限公司 | Sterilization composition containing flutolanil |
| CN102626074A (en) * | 2012-04-01 | 2012-08-08 | 广西田园生化股份有限公司 | Ultra-low volume liquid containing flutolanil |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101637162A (en) * | 2009-09-08 | 2010-02-03 | 深圳诺普信农化股份有限公司 | Sterilization composition containing flutolanil |
| CN102626074A (en) * | 2012-04-01 | 2012-08-08 | 广西田园生化股份有限公司 | Ultra-low volume liquid containing flutolanil |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103073349A (en) * | 2012-12-29 | 2013-05-01 | 广东中迅农科股份有限公司 | Drug fertilizer granules for preventing rice damping-off and preparation method thereof |
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