CN102765971A - Amino acid fertilizer and preparation method thereof - Google Patents

Amino acid fertilizer and preparation method thereof Download PDF

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Publication number
CN102765971A
CN102765971A CN2012102151416A CN201210215141A CN102765971A CN 102765971 A CN102765971 A CN 102765971A CN 2012102151416 A CN2012102151416 A CN 2012102151416A CN 201210215141 A CN201210215141 A CN 201210215141A CN 102765971 A CN102765971 A CN 102765971A
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Prior art keywords
amino acid
acid fertilizer
parts
preparation
subtilis
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CN2012102151416A
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CN102765971B (en
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张怀忠
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Chongqing Ruibao Agricultural Industry Group Co Ltd
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Chongqing Ruibao Agricultural Industry Group Co Ltd
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    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

Abstract

The invention discloses an amino acid fertilizer and a preparation method thereof. The amino acid fertilizer comprises the following components, by weight: 1-2 parts of ferment bacteria, 20-30 parts of livestock manure, and 50-70 parts of sweet wormwood slag. The ferment bacteria comprise Bacillus subtilis RB and commercially available ferment bacteria. The Bacillus subtilis RB is preserved in China General microbiological Culture Collection Center, and has a preservation number of CGMCC NO.6268 and high effectiveness on treating sweet wormwood slag. The prepared amino acid fertilizer contains abundant nutrient, and is rich in amino acids, so as to change waste into treasure, and provide premium amino acid fertilizer for agricultural and forestry production.

Description

Amino acid fertilizer and preparation method thereof
Technical field
The invention belongs to fertilizer field, particularly amino acid fertilizer also relates to the preparation method of this fertilizer.
Background technology
Sweet wormwood ( Artemisia annua L.) be the starting material that extract Artemisinin, develop into about 120,000 mu to Chongqing City's sweet wormwood cultivated area in 2005, planting area mainly contains ten thousand states, tenth of the twelve Earthly Branches sun, Fengdu, Fuling, WuLong, Peng River, Qianjiang, Liangping etc.At present, get involved the holding group of Holley of sweet wormwood industry, logical and pharmacy, Beijing gold can, the permanent new company in Beijing all has Artemisinin throughput.Artemisinin industry flourish produced a large amount of sweet wormwood slags, feed veterinary drug supervision institute and Chengdu oil and foodstuffs feeding quality supervision and inspection center of national grain and oil office analysis revealed through the Chongqing City: contain compositions such as crude protein (about 21%), robust fibre (about 11%) and amino acid in the sweet wormwood slag.At present, the sweet wormwood slag is directly burnt or works as garbage loading embeading, have the Chinese medicine irritating smell to produce in the combustion processes unavoidably, influenced peripheral resident's orthobiosis, and directly landfill has caused the waste of resource.Therefore, be badly in need of a kind of method of rationally utilizing the sweet wormwood slag, realize turning waste into wealth.
Summary of the invention
One of the object of the invention is to provide amino acid fertilizer, and abundant nutrients contains organic matter, organonitrogen phosphorus potassium, humic acid and amino acid; Can improve the output of farm crop after using, reduce heavy metal in soil content, have the effect of improving soil simultaneously.Two of the object of the invention is to provide the preparation method of amino acid fertilizer, and its preparation method is simple, does not need specific installation, and cost is low.
For realizing above-mentioned purpose, technical scheme of the present invention is:
1. amino acid fertilizer, composed of the following components by weight:
1～2 part of enzymatic microorganism;
20～30 parts of fowl and animal excrements;
50～70 parts of sweet wormwood slags;
Said enzymatic microorganism by subtilis ( Bacillus subtilis) RB and commercially available enzymatic microorganism composition, said subtilis RB is preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center, and preserving number is CGMCG NO. 6268.
The characteristic of subtilis RB of the present invention is following: 0.7～0.8 * 2～3 microns of individual cells, uniform coloring.No pod membrane, peritrichous can move.Gram-positive microorganism, 0.6～0.9 * 1.0～1.5 microns of gemma, oval to column, be positioned at thalline central authorities or inclined to one side slightly, gemma forms the back thalline and does not expand.The bacterium colony surface irregularity is opaque, dirty white or little yellow, and when growing in the liquid medium within, normal formation wrinkle mould, aerophil.Available protein, multiple sugar and starch decompose tryptophane and form indoles.
The physio-biochemical characteristics of subtilis RB of the present invention: do not have in the cell and gather-beta-hydroxy, produce acetyl methyl carbinol, the VP reacting positive, VP liquid pH is 6.3～6.7; Catalase is positive, oxidase positive; Lecithinase is negative, reduction nitrate salt; At massfraction is that 7% NaCl and pH grow under 5.8 the condition, in anaerobic culture medium, does not grow aerobiosis.Can utilize glucose, wood sugar and N.F,USP MANNITOL to produce acid, but can not utilize pectinose to produce acid, suckling to be in the reindeer moss peptonizes reduction reaction.
Preferably, said amino acid fertilizer, composed of the following components by weight:
1.2 parts of enzymatic microorganism;
25 parts of fowl and animal excrements;
60 parts of sweet wormwood slags.
Preferred, the weight ratio of said subtilis RB and commercially available enzymatic microorganism is 1～2:5.
2. the preparation method of said amino acid fertilizer; May further comprise the steps: subtilis RB, fowl and animal excrement and sweet wormwood slag are mixed by proportioning; Be 60-70 ℃ in temperature then, carried out open aerobic solid-state fermentation 6-7 days, get into drying, pulverizing, granulation, screening through transfer system again; Test package gets amino acid fertilizer.
Preferably, but be back in the fermentation step again in the said dry waste gas suction pond that produces.
Beneficial effect of the present invention: the invention discloses amino acid fertilizer; With the sweet wormwood slag is main raw material, makes amino acid fertilizer abundant nutrients with the enzymatic microorganism fermentation that contains subtilis RB; Wherein organic massfraction >=35%, organonitrogen phosphorus potassium massfraction >=6%; Effectively bacterium reaches 2.5 hundred million, humic acid massfraction >=15%, amino acid masses mark >=10%.The invention also discloses the preparation method of amino acid fertilizer, its preparation technology is simple, does not need specific installation, becomes to produce cost low.
The subtilis RB that the present invention uses breeds through bacterium in a large number; Produce tropina; Thereby the generation dynamics is very strong, render a service the very high water decomposition enzyme that adds; Can be at short notice agricultural solid residue be carried out the saccharification decomposition and protein decomposes, Mierocrystalline cellulose decomposes, generate glucose, alcohol, amino acid etc., for useful microbe in the agricultural wastes provides better culture base-material; Subtilis RB can also promote the hurried propagation of the actinomycetes in the agricultural wastes, and a large amount of mikrobes promote humic acid to form.Humic acid is a polymer substance, and the alkali replacement amount is high, has good cnmplexation adsorbability, heavy metal is had the strong characteristics of adsorbing with selection of adsorptive power, thereby form very strong attenuation effect.
Description of drawings
Fig. 1 is the preparation technology of amino acid fertilizer.
Culture presevation
Subtilis among the present invention ( Bacillus subtilis) RB is from occurring in nature separation screening useful microbe; Obtain through purification and rejuvenation, improvement of genes; Send China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation, deposit number is CGMCG NO.6268, and the address is positioned at No. 3 institutes of microbiology of the Chinese Academy of Sciences in Yard 1, BeiChen xi Road, Chaoyang District, Beijing City; Preservation date is on 06 21st, 2012, classification called after subtilis ( Bacillus subtilis), symbol is RB.
Embodiment
Below will combine embodiment that the present invention is carried out detailed description.The experimental technique of unreceipted actual conditions in the preferred embodiment is usually according to normal condition.
Embodiment 1, subtilis ( Bacillus subtilis) separation of RB
Will be from occurring in nature separation screening useful microbe 10g and nutrition base uniform mixing; Contain W-Gum 5～10g, brown sugar 5～10g and wheat bran 10～20g in the nutrition base; PH6.0～7.0, humidity are 30～35%, add the uncommon imines 0.005～0.05g of azanol or second again; 25～35 ℃ of temperature, the temperature difference is 8～10 ℃, cultivates 2～3 days, filters out subtilis RB with transfering loop.Wherein the nutrition base also can comprise fish meal 3～5g, dish cake 3～5g or polished rice chaff 35～50g.
The cultivation of embodiment 2, subtilis RB
Subtilis RB substratum: sucrose 50g, (NH 4) 2SO 46g, K 2SO 42.5g, CaSO 42.5g, agar powder 15g and zero(ppm) water 1000mL.
Culture condition is: temperature is 30 ℃, and pH is 5.8.
The preparation of embodiment 3, amino acid fertilizer
Subtilis RB is mixed with commercially available enzymatic microorganism and promptly gets enzymatic microorganism of the present invention by 1～2:5 part by weight.
The raw material of amino acid fertilizer, composed of the following components by weight:
Prescription 1: 1 part of enzymatic microorganism, 50 parts of 20 parts of fowl and animal excrements and sweet wormwood slags.
Prescription 2: 2 parts of enzymatic microorganism, 70 parts of 30 parts of fowl and animal excrements and sweet wormwood slags.
Prescription 3: 1.2 parts of enzymatic microorganism, 60 parts of 25 parts of fowl and animal excrements and sweet wormwood slags.
The preparation method of amino acid fertilizer, concrete steps are following:
The preparation method 1: enzymatic microorganism, fowl and animal excrement and sweet wormwood slag mix by said ratio; Carrying out in temperature after the stirring is under 60 ℃ of conditions, and open aerobic solid-state fermentation 7 days gets into drying, pulverizing, granulation, screening through transfer system again; Get amino acid fertilizer, process step is as shown in Figure 1.The ERG that produces in the drying step is in fermentation step.
The preparation method 2: preparation method 2 is identical with preparation method 1, and difference is that fermentation condition is is under 70 ℃ of conditions in temperature, open aerobic solid-state fermentation 6 days.
In the amino acid fertilizer of method for preparing, organic massfraction >=35%, organonitrogen phosphorus potassium massfraction >=6%, effectively bacterium reaches 2.5 hundred million, humic acid massfraction >=15%, amino acid masses mark >=10%.
The amino acid fertilizer of above-mentioned preparation is used fertilizer as soil, use 100 kilograms of amino acid fertilizers for every mu, use after 1 month and detected by environmental monitoring station, detect heavy metal in soil content, the result is as shown in table 1.
  
Table 1, heavy metal in soil content detection result
Figure 580074DEST_PATH_IMAGE002
Can know by table 1, use the amino acid fertilizer that the present invention makes after, the wherein heavy metal content such as lead, cadmium, chromium, arsenic, mercury in the soil are far below international standard, National standard.
Pollutent control criterion in the agricultural mud (GB4284-84)/(mg/kg), see table 2.
Pollutent control criterion in table 2, the agricultural mud
Figure 559532DEST_PATH_IMAGE004
Can know by table 1 and table 2, use the amino acid fertilizer that the present invention makes after, can reduce the content of heavy metal in soil, mainly be through amino acid and heavy metal generation complexing action, thereby reduce the content of heavy metal in soil.
The application of embodiment 4, amino acid fertilizer
Application examples 1
The amino acid fertilizer that makes is used for the base manure of citrus and ginger, and citrus grows fine, fast growth, and the fruit sugariness increases, and mouthfeel is good; Compare output with citrus, the ginger of valency fertilizer and increase by 30% with using.
Application examples 2
The amino acid fertilizer of the present invention's preparation is carried out manure trial on vegetables:
The amino acid fertilizer of the present invention's preparation is demonstrated on pumpkin, cucumber, wild cabbage, radish vegetables, and test equates with fertile cost with conventional cultivation with fertile cost design.The present invention that the radish mu value of making an offer to buy is 190 yuan prepares amino acid fertilizer, and plant strain growth is good, and blade is light green, and drought-resistant ability is strong; The amino acid fertilizer that the cucumber mu value of making an offer to buy is 260 yuan, plant strain growth is healthy and strong, and upright blade is light green, and drought resistance is strong, and precocious 3～5 days, fruit bar shaped and quality made moderate progress; The amino acid fertilizer that the pumpkin mu value of making an offer to buy is 165 yuan, tendril is healthy and strong, and leaf is light green, and drought-resistant ability is strong, and fruit marketability is good, meat powder, sweet, ripening stage basically identical; The amino acid fertilizer that the wild cabbage mu value of making an offer to buy is 260 yuan, the leaf look light green, robust growth.
Application examples 3
The amino acid fertilizer degrading pesticide residues function of the present invention's preparation:
Beijing area tomato, romaine lettuce test nitrate content test result shows, executes the nitrate salt average content significantly the is lower than nutrient chemical fertilizer disposition of etc.ing behind the amino acid fertilizer of the present invention's preparation, and wherein, the tomato nitrate content reduces by 9.5%, romaine lettuce reduction by 8.1%.
The nitrate salt average content of using the amino acid fertilizer of the present invention's preparation in the Chinese cabbage test of Shandong is 416.6mg/kg, and the average content of using chemical fertilizer is 764.6mg/kg.
Application examples 4
The effect that the amino acid fertilizer of the present invention's preparation is improved the soil:
Experiment and demonstration data according to Beijing, Shandong; Use bio-fertilizer of the present invention and improved the soil organism, full nitrogen and available phosphorus content; Respectively handling the soil analysis result before Tongzhou District, Beijing tomato test results shows; The soil organism that the amino acid fertilizer of the present invention preparation is handled, full nitrogen and available phosphorus are respectively 1.34%, 0.106% and 82.4mg/kg, and that waits that the nutrient chemical fertilizer handles then is respectively 1.16%, 0.087% and 78.3mg/kg, and three indexs all are higher than to use and wait the processing of nutrient chemical fertilizer.
Explanation is at last; Above embodiment is only unrestricted in order to technical scheme of the present invention to be described; Although through invention has been described with reference to the preferred embodiments of the present invention; But those of ordinary skill in the art should be appreciated that and can make various changes to it in form with on the details, and the spirit and scope of the present invention that do not depart from appended claims and limited.

Claims (5)

1. amino acid fertilizer is characterized in that, and is composed of the following components by weight:
1～2 part of enzymatic microorganism;
20～30 parts of fowl and animal excrements;
50～70 parts of sweet wormwood slags;
Said enzymatic microorganism by subtilis ( Bacillus subtilis) RB and commercially available enzymatic microorganism form, said subtilis RB is preserved in Chinese common micro-organisms culture presevation administrative center, and preserving number is CGMCG NO. 6268.
2. according to the said amino acid fertilizer of claim 1, it is characterized in that, composed of the following components by weight:
1.2 parts of enzymatic microorganism;
25 parts of fowl and animal excrements;
60 parts of sweet wormwood slags.
3. according to claim 1 or 2 said amino acid fertilizers, it is characterized in that: the weight ratio of said subtilis RB and commercially available enzymatic microorganism is 1～2:5.
4. the preparation method of each said amino acid fertilizer of claim 1-3; It is characterized in that; May further comprise the steps: enzymatic microorganism, fowl and animal excrement and sweet wormwood slag are mixed by proportioning, are 60-70 ℃ in temperature then, carry out open aerobic solid-state fermentation 6-7 days; Get into drying, pulverizing, granulation, screening through transfer system again, get amino acid fertilizer.
5. according to the preparation method of the said amino acid fertilizer of claim 4, it is characterized in that the ERG that said drying step produces is in fermentation step.
CN201210215141.6A 2012-06-27 2012-06-27 Amino acid fertilizer and preparation method thereof Expired - Fee Related CN102765971B (en)

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103224425A (en) * 2013-04-18 2013-07-31 陆少英 Compound amino acid biological liquid fertilizer and preparation method thereof
CN103319245A (en) * 2013-06-27 2013-09-25 苏州仁成生物科技有限公司 Compound microbial fertilizer for preventing and treating downy mildew of spinach and preparation method thereof
CN103396241A (en) * 2013-08-08 2013-11-20 苏州市相城区兴湖生态农业专业合作社 Composite bactericidal insecticidal fertilizer used for controlling spinach downy mildew, and preparation method thereof
CN104072220A (en) * 2014-07-04 2014-10-01 重庆鑫禾生物科技有限责任公司 Artemisia apiacea bio-organic fertilizer and preparation method thereof
CN105948972A (en) * 2016-04-25 2016-09-21 韩德辉 Amino acid fertilizer and preparation method and application thereof
CN107721605A (en) * 2017-11-13 2018-02-23 广西肥源生物科技有限公司 A kind of amino acid water-soluble fertilizer and preparation method thereof
CN110845275A (en) * 2019-10-18 2020-02-28 贵州鼎亿生物科技有限公司 Sweet wormwood mixed fertilizer and preparation method thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6315807B1 (en) * 2000-03-28 2001-11-13 Council Of Scientific And Industrial Research Formulation useful as a nitrification and urease inhibitor and a method of producing the same
CN101200390A (en) * 2007-12-26 2008-06-18 李珍发 Production technique of organic fertilizer
CN101215532A (en) * 2007-12-27 2008-07-09 重庆市瑞宝农业开发有限公司 Bacillus megaterium and its application and application method in ferment bacteria
CN101244955A (en) * 2007-12-27 2008-08-20 重庆市瑞宝农业开发有限公司 Biological fertilizer and manufacture method thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6315807B1 (en) * 2000-03-28 2001-11-13 Council Of Scientific And Industrial Research Formulation useful as a nitrification and urease inhibitor and a method of producing the same
CN101200390A (en) * 2007-12-26 2008-06-18 李珍发 Production technique of organic fertilizer
CN101215532A (en) * 2007-12-27 2008-07-09 重庆市瑞宝农业开发有限公司 Bacillus megaterium and its application and application method in ferment bacteria
CN101244955A (en) * 2007-12-27 2008-08-20 重庆市瑞宝农业开发有限公司 Biological fertilizer and manufacture method thereof

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103224425A (en) * 2013-04-18 2013-07-31 陆少英 Compound amino acid biological liquid fertilizer and preparation method thereof
CN103224425B (en) * 2013-04-18 2014-12-03 陆少英 Compound amino acid biological liquid fertilizer and preparation method thereof
CN103319245A (en) * 2013-06-27 2013-09-25 苏州仁成生物科技有限公司 Compound microbial fertilizer for preventing and treating downy mildew of spinach and preparation method thereof
CN103396241A (en) * 2013-08-08 2013-11-20 苏州市相城区兴湖生态农业专业合作社 Composite bactericidal insecticidal fertilizer used for controlling spinach downy mildew, and preparation method thereof
CN104072220A (en) * 2014-07-04 2014-10-01 重庆鑫禾生物科技有限责任公司 Artemisia apiacea bio-organic fertilizer and preparation method thereof
CN105948972A (en) * 2016-04-25 2016-09-21 韩德辉 Amino acid fertilizer and preparation method and application thereof
CN107721605A (en) * 2017-11-13 2018-02-23 广西肥源生物科技有限公司 A kind of amino acid water-soluble fertilizer and preparation method thereof
CN110845275A (en) * 2019-10-18 2020-02-28 贵州鼎亿生物科技有限公司 Sweet wormwood mixed fertilizer and preparation method thereof

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