CN102747116A - Production technology for extracting soybean isoflavone aglycone through enzymatic hydrolysis method - Google Patents
Production technology for extracting soybean isoflavone aglycone through enzymatic hydrolysis method Download PDFInfo
- Publication number
- CN102747116A CN102747116A CN2012101909309A CN201210190930A CN102747116A CN 102747116 A CN102747116 A CN 102747116A CN 2012101909309 A CN2012101909309 A CN 2012101909309A CN 201210190930 A CN201210190930 A CN 201210190930A CN 102747116 A CN102747116 A CN 102747116A
- Authority
- CN
- China
- Prior art keywords
- hydrolysis
- glucosidase
- isoflavone
- ethanol
- production technique
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention provides a production technology for extracting soybean isoflavone aglycone through an enzymatic hydrolysis method, and belongs to the technical field of processing and extraction of soybeans. The technology comprises the following steps: immersing degreased soybean meal according to a material-to-water ratio of 5-10:1; adding ethanol to an immersion solution until the mass concentration of ethanol in the ethanol added immersion solution is 10%; hydrolyzing through adding superhigh temperature beta-glucosidase to the immersion solution at 90-120DEG C for 1-2h under slowly stirring; carrying out solid-liquid separation after the hydrolysis; extracting through adding 60-80% ethanol to the resulting solid precipitate; filtering the resulting extract solution by a film to obtain a permeation solution; and carrying out protein removal, reduced pressure concentration and drying on the permeation solution to obtain the isoflavone aglycone. The technology which utilizes enzymological characteristics comprising the high temperature resistance, the heat stability and the like of the superhigh temperature beta-glucosidase enables the superhigh temperature beta-glucosidase to be applied to the extraction and the preparation of the soybean isoflavone aglycone, so the production efficiency is improved, the production cost is reduced, and the obtained isoflavone aglycone is sugar-free isoflavone having a high absorptivity and a strong biological activity.
Description
Technical field
The present invention relates to big beans processing extractive technique field, technical field, specifically a kind of enzymolysis process extracts the production technique of isoflavone genin.
Background technology
General, isoflavone genin adopts following method to make more, but all has certain defective and deficiency: basic hydrolysis: aglycon structural instability, facile hydrolysis; Acid hydrolysis: hydrolysis efficiency is high; But be unfavorable for industrial production under the strong acid condition, corrosive equipment causes environmental pollution, and aglycon stability is worth suspecting; Enzymic hydrolysis: speed of response is fast, mild condition, and aglycon stability is high; But aglycon solubleness in water is low, and general enzymic hydrolysis is not high.
The soybean isoflavones of finding at present has 12 kinds, but most of instability.Present stage, the application of soybean isoflavones, biological function research are mainly concentrated on daidzin and the Genistoside.In the crude soya bean seed, the soybean isoflavones of 97-98% is that the glucosides form with β-D-mating type exists.Human body is low to the glucosides receptivity of mating type, and sugar-free isoflavone aglycone receptivity is strong, all, the hydrolysis of iso-flavone glucoside is most important.Enzymic hydrolysis: speed of response is fast, mild condition, and aglycon stability is high; But aglycon solubleness in water is low, and general enzymic hydrolysis is not high.
Summary of the invention
Technical assignment of the present invention is the deficiency that solves prior art, provides a kind of enzymolysis process to extract the production technique of isoflavone genin.
Technical scheme of the present invention realizes that by following mode this enzymolysis process extracts the production technique of isoflavone genin, and its process step is: defatted soybean meal soaks; Material-water ratio 5～10: 1, in soak solution, add ethanol to ethanol and account for 10% of total soak solution mass concentration, soak solution adds the enzymic hydrolysis of ultrahigh-temperature beta-glucoside; Hydrolysis temperature is 90 ℃～120 ℃, and slowly stir on hydrolysis limit, limit, behind hydrolysis time 1～2h; Solid-liquid separation, Gu the shape throw out adds 60%～80% ethanol extracting, extract is through membrane filtration; Must see through liquid, see through liquid and after deproteinated, concentrating under reduced pressure, drying, get isoflavone.
Described membrane filtration adopts the inorganic ceramic membrane filtration.
Soak solution hydrolysis PH scope is 4.5～8.0.
Described ultrahigh-temperature beta-glucosidase addition is the 0.5U/mL soak solution.
Described ultrahigh-temperature beta-glucosidase adopts the yeast beta-glucosidase.
Defatted soybean meal is from the tankage soybean meal of oily factory.
The inorganic ceramic film that membrane filtration adopts is held back the macromole prolamine.
The beneficial effect that the present invention is compared with prior art produced is:
The production technique that this enzymolysis process extracts isoflavone genin is utilized zymetology characteristics such as high temperature resistant, the thermostability of ultrahigh-temperature beta-glucosidase; Be applied to extraction, the preparation technology of isoflavone genin; Not only enhance productivity; Reduce production costs, and products obtained therefrom is the sugar-free type NOVASOY 400 that specific absorption is high, biological activity is strong.Sugar-free isoflavone aglycone biological function: anti-oxidant, remove radical, preventing cardiovascular disease; Hormonal activity, to mammary cancer, prostate cancer, climacteric syndrome has prophylactic effect; Stop blood vessel hyperplasia, suppress multiple growth of cancer cells.
Ultrahigh-temperature beta-glucosidase: the high temperature resistant beta-glucosidase of pyrococcus furiosus CelB genetic expression; Can be operated under 65-120 ℃, and enzymic activity is stable, this moment, the aglycon water solubility was big; Can increase the output of sugar-free isoflavone aglycone, shorten the production cycle.And under 10% ethanol environment, this enzymic activity slightly improves.
The used enzyme of production technique that this enzymolysis process extracts isoflavone genin is the ultrahigh-temperature beta-glucosidase, in the time of 120 ℃, still has higher enzyme and lives; And the maximum temperature of used enzyme is 90 ℃ on the conventional art, and temperature is high more, and the water-soluble soybean isoflavone of stripping is many more from soybean meal, helps transforming the output isoflavone genin more.
The used enzyme of production technique that this enzymolysis process extracts isoflavone genin is that foodstuff additive are used enzyme, is the zymic meta-bolites, has security, decomposes the isoflavone genin that produces and can be used for food, medicine and healthcare products etc.; And used enzyme source can not guarantee to be used for foodstuff additive in bacterium on the conventional art, possibly contain certain toxicity, has limited the application of isoflavone genin.
The used enzyme reaction solution of production technique that this enzymolysis process extracts isoflavone genin is the aqueous solution, need not add other additives such as any composition or damping fluid.
The production technique enzyme reaction time that this enzymolysis process extracts isoflavone genin is few, and the enzyme reaction time is merely 1～2 hour; And on the conventional art enzyme reaction time be 3～5 hours, so this technology saves time, favourable enlargement of scale production brings good economic benefit.
This enzymolysis process extracts the production technique of isoflavone genin on the efficient of extracting isoflavone genin, be higher than conventional art.
Description of drawings
Accompanying drawing 1 is the amount of hydrolysis structural representation of the daidzein of detected result of the present invention;
Accompanying drawing 2 is amount of hydrolysis structural representations of the genistein of detected result of the present invention.
Embodiment
Extract following detailed description of production technique work of isoflavone genin down in the face of enzymolysis process of the present invention.
Enzymolysis process of the present invention extracts the production technique of isoflavone genin; Its process step is: defatted soybean meal is from the tankage soybean meal of oily factory; Defatted soybean meal soaks, material-water ratio 5～10: 1, and in soak solution, add ethanol to ethanol and account for 10% of total soak solution mass concentration; Soak solution adds the enzymic hydrolysis of ultrahigh-temperature beta-glucoside, and the ultrahigh-temperature beta-glucosidase adopts the yeast beta-glucosidase.Ultrahigh-temperature beta-glucosidase addition is the 0.5U/mL soak solution, and hydrolysis temperature is 90 ℃～120 ℃, and soak solution hydrolysis PH scope is 4.5～8.0; Slowly stir on hydrolysis limit, limit, behind hydrolysis time 1～2h, and solid-liquid separation; Gu the shape throw out adds 60%～80% ethanol extracting, extract is held back the macromole prolamine through the inorganic ceramic membrane filtration; Must see through liquid, see through liquid and after deproteinated, concentrating under reduced pressure, drying, get isoflavone.
Claims (5)
1. enzymolysis process extracts the production technique of isoflavone genin, it is characterized in that process step is: defatted soybean meal soaks, material-water ratio 5～10: 1; In soak solution, add ethanol to ethanol and account for 10% of total soak solution mass concentration, soak solution adds the enzymic hydrolysis of ultrahigh-temperature beta-glucoside, and hydrolysis temperature is 90 ℃～120 ℃; Slowly stir on hydrolysis limit, limit, behind hydrolysis time 1～2h, and solid-liquid separation; Gu the shape throw out adds 60%～80% ethanol extracting; Extract must see through liquid through membrane filtration, sees through liquid and after deproteinated, concentrating under reduced pressure, drying, gets isoflavone.
2. enzymolysis process according to claim 1 extracts the production technique of isoflavone genin, it is characterized in that described membrane filtration adopts the inorganic ceramic membrane filtration.
3. enzymolysis process according to claim 1 extracts the production technique of isoflavone genin, it is characterized in that soak solution hydrolysis PH scope is 4.5～8.0.
4. enzymolysis process according to claim 1 extracts the production technique of isoflavone genin, it is characterized in that described ultrahigh-temperature beta-glucosidase addition is the 0.5U/mL soak solution.
5. enzymolysis process according to claim 1 extracts the production technique of isoflavone genin, it is characterized in that described ultrahigh-temperature beta-glucosidase adopts the yeast beta-glucosidase.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012101909309A CN102747116A (en) | 2012-06-12 | 2012-06-12 | Production technology for extracting soybean isoflavone aglycone through enzymatic hydrolysis method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012101909309A CN102747116A (en) | 2012-06-12 | 2012-06-12 | Production technology for extracting soybean isoflavone aglycone through enzymatic hydrolysis method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102747116A true CN102747116A (en) | 2012-10-24 |
Family
ID=47027607
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012101909309A Pending CN102747116A (en) | 2012-06-12 | 2012-06-12 | Production technology for extracting soybean isoflavone aglycone through enzymatic hydrolysis method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102747116A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102994583A (en) * | 2012-12-10 | 2013-03-27 | 天津大学 | Production method of soybean isoflavone glycoside |
| CN109971801A (en) * | 2018-11-28 | 2019-07-05 | 山东省分析测试中心 | Application of the high-speed counter-current chromatograph in the conversion of glycoside ingredients Biogenic |
| CN110372770A (en) * | 2018-04-12 | 2019-10-25 | 耿兆翔 | The highly effective extraction method of natural hydroxyl or/and carboxyl compound |
| CN110846354A (en) * | 2019-11-29 | 2020-02-28 | 邯郸学院 | Preparation method of blackberry lily isoflavone aglycone |
| CN110951709A (en) * | 2019-12-06 | 2020-04-03 | 鹤山市东古调味食品有限公司 | Complex enzyme and application thereof |
| CN115669862A (en) * | 2022-11-15 | 2023-02-03 | 苏州金记食品有限公司 | Preparation method of bean curd gel rich in soybean isoflavone |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101200744A (en) * | 2007-12-07 | 2008-06-18 | 南京师范大学 | High-effective clean method for preparing soybean isoflavone aglycone |
| CN101974577A (en) * | 2010-09-27 | 2011-02-16 | 徐州技源天然保健品有限公司 | Novel production process for extracting soy isoflavones aglycones |
-
2012
- 2012-06-12 CN CN2012101909309A patent/CN102747116A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101200744A (en) * | 2007-12-07 | 2008-06-18 | 南京师范大学 | High-effective clean method for preparing soybean isoflavone aglycone |
| CN101974577A (en) * | 2010-09-27 | 2011-02-16 | 徐州技源天然保健品有限公司 | Novel production process for extracting soy isoflavones aglycones |
Non-Patent Citations (6)
| Title |
|---|
| 《Eur. J. Biochem.》 19931231 Serve W. M. KENGEN等 Purification and characterization of an extremely thermostable beta-glucosidase from the hyperthermophilic archaeon Pyrococcus furiosus 305-312 1-5 第213卷, * |
| 《Journal of Agricultural and Food Chemistry》 20120118 Soo-Jin Yeom等 Hydrolysis of Isoflavone Glycosides by a Thermostable beta-Glucosidase from Pyrococcus furiosus 1535-1541 1-5 第60卷, * |
| 《食品研究与开发》 20051231 许晶 等 beta-葡萄糖苷酶的研究进展 183-186 1-5 第26卷, 第6期 * |
| SERVE W. M. KENGEN等: "Purification and characterization of an extremely thermostable β-glucosidase from the hyperthermophilic archaeon Pyrococcus furiosus", 《EUR. J. BIOCHEM.》, vol. 213, 31 December 1993 (1993-12-31), pages 305 - 312 * |
| SOO-JIN YEOM等: "Hydrolysis of Isoflavone Glycosides by a Thermostable β-Glucosidase from Pyrococcus furiosus", 《JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY》, vol. 60, 18 January 2012 (2012-01-18), pages 1535 - 1541 * |
| 许晶 等: "β-葡萄糖苷酶的研究进展", 《食品研究与开发》, vol. 26, no. 6, 31 December 2005 (2005-12-31), pages 183 - 186 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102994583A (en) * | 2012-12-10 | 2013-03-27 | 天津大学 | Production method of soybean isoflavone glycoside |
| CN110372770A (en) * | 2018-04-12 | 2019-10-25 | 耿兆翔 | The highly effective extraction method of natural hydroxyl or/and carboxyl compound |
| CN109971801A (en) * | 2018-11-28 | 2019-07-05 | 山东省分析测试中心 | Application of the high-speed counter-current chromatograph in the conversion of glycoside ingredients Biogenic |
| CN110846354A (en) * | 2019-11-29 | 2020-02-28 | 邯郸学院 | Preparation method of blackberry lily isoflavone aglycone |
| CN110846354B (en) * | 2019-11-29 | 2021-05-04 | 邯郸学院 | Preparation method of blackberry lily isoflavone aglycone |
| CN110951709A (en) * | 2019-12-06 | 2020-04-03 | 鹤山市东古调味食品有限公司 | Complex enzyme and application thereof |
| CN115669862A (en) * | 2022-11-15 | 2023-02-03 | 苏州金记食品有限公司 | Preparation method of bean curd gel rich in soybean isoflavone |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102747116A (en) | Production technology for extracting soybean isoflavone aglycone through enzymatic hydrolysis method | |
| CN105482890B (en) | A kind of method of microwave-ultrasonic auxiliary extracting linseed oil by water-enzyme process | |
| Yan et al. | Three-phase partitioning as an elegant and versatile platform applied to nonchromatographic bioseparation processes | |
| CN100485040C (en) | Process for preparing shiitake mushroom extract | |
| CN103044570A (en) | Extracting process for efficiently extracting polysaccharide of enteromorpha | |
| CN101967437B (en) | Method for producing small peptide beverages by fermenting yellow serofluid produced by processing beancurd | |
| JP5967754B2 (en) | Usage of yeast extract extraction residue | |
| CN104211827B (en) | A kind of method extracting polysaccharide from Hericium erinaceus (Bull. Ex Fr.) Pers. dreg | |
| CN103864949A (en) | Method for preparing maca polysaccharide and maca alkaloid by use of membrane separation technique | |
| CN101103119A (en) | Manufacturing method of kaempferol | |
| CN102334702B (en) | Production method for laver protein and polysaccharide nutrient powder | |
| CN102816633A (en) | Method for synchronously extracting corn germ oil and separation protein by using alcohol-assisted enzyme method | |
| CN100586442C (en) | Method of producing glossy ganoderma polypeptide product | |
| CN106086123A (en) | A kind of application ionic liquid improves the water miscible method of flavone compound | |
| CN104073537A (en) | Preparation method for active collagen from compound protease | |
| CN101200744B (en) | High-effective clean method for preparing soybean isoflavone aglycone | |
| CN101912445B (en) | Method for extracting general flavonoids from toona sinensis leaves under assistance of surfactant | |
| CN105175568A (en) | Ginkgo seed polysaccharide extraction method and ginkgo seed polysaccharide product | |
| Huang et al. | Enzymolysis kinetics of garlic powder with single frequency countercurrent ultrasound pretreatment | |
| Qin et al. | Extraction and utilization of active substances from edible fungi substrate and residue: A review | |
| CN101768613B (en) | Preparation method of flavone genin of seabuckthorn leaves | |
| GB1356449A (en) | Process for extracting water soluble components of the hyphae of edible fungi | |
| CN103614429B (en) | Method for transforming gingko pollen flavonoid glycoside employing microorganisms | |
| Tremblay et al. | Extraction technologies for proteins and peptides | |
| CN103478784A (en) | Method for preparing walnut kernel polypeptide through bacillus natto fermentation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20121024 |