CN101974577A - Novel production process for extracting soy isoflavones aglycones - Google Patents

Novel production process for extracting soy isoflavones aglycones Download PDF

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Publication number
CN101974577A
CN101974577A CN 201010294665 CN201010294665A CN101974577A CN 101974577 A CN101974577 A CN 101974577A CN 201010294665 CN201010294665 CN 201010294665 CN 201010294665 A CN201010294665 A CN 201010294665A CN 101974577 A CN101974577 A CN 101974577A
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CN
China
Prior art keywords
solution
production technique
extraction
extracting
isoflavone genin
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CN 201010294665
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Chinese (zh)
Inventor
张艳
蒋永红
曹礼群
李五洲
李新晴
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JIYUAN NATURAL HEALTH PRODUCTS CO Ltd XUZHOU
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JIYUAN NATURAL HEALTH PRODUCTS CO Ltd XUZHOU
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Priority to CN 201010294665 priority Critical patent/CN101974577A/en
Publication of CN101974577A publication Critical patent/CN101974577A/en
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Abstract

The invention relates to a novel production process for extracting soy isoflavones aglycones. The production process in the invention comprises the following steps of: smashing soy embryos or soy bean meal which serves as a raw material, extracting required ingredients, concentrating, removing proteins, performing column absorption and analysis, concentrating for the second time, performing enzymolysis, extracting a solvent, concentrating for the third time, freeze drying and obtaining the soy isoflavones aglycones. The novel production process for extracting the soy isoflavones aglycones is convenient to operate, high in repeatability and yield and suitable for industrial production; the enzymolysis method is adopted for converting the aglycones, so that the conversion rate is high and the process avoids using poison andharmful solvents; and electrolyte is used for settling the proteins, so that impurity removal is efficient and quick.

Description

A kind of production technique of novel extraction isoflavone genin
Technical field
The invention belongs to natural product extraction technology, particularly a kind of production technique of novel extraction isoflavone genin.
Background technology
Soybean isoflavones is that the natural quality soybean is prepared from through the modern high technology means of purification, has strengthening immunity, anti-oxidant, function such as anti-aging, antibiotic in advance.At aspects such as the sickness rate that reduces the middle aged and aged women osteoporosis, elimination climacteric syndromes extraordinary effect is arranged.Multinomial studies show that, soybean isoflavones still are that the women prevents mammary cancer, the best health care nutriment of uterus carcinoma.Yet existing extractive technique is unsatisfactory, have that productive rate is low, complicated operation,, extract problems such as transformation efficiency is low, lyophilize weak effect.
Summary of the invention
In extracting the isoflavone genin process, there to be some unfavorable problems in order solving, to the invention provides a kind of production technique of novel extraction isoflavone genin, this invented technology productive rate height, simple operation, extraction transformation efficiency height from soy material.
Technical scheme of the present invention is: concrete composition of the present invention is daidzin, daidzin, Genistoside, daidzein, Daidzein, genistein.This invented technology step is:
(1) raw material is prepared: after soybean germ or bean cake powder essence to particle diameter are 0.5mm-5.0mm, standby;
(2) enzyme decomposes: add organized enzyme, controlled temperature is 40 ℃-50 ℃, and enzymolysis time is 24-72 hour;
(3) extract: with concentration is the alcohol reflux twice of 60%-80%, and solvent and raw material envelope-bulk to weight ratio are 5-10: 1, extract temperature at 70 ℃-100 ℃, alcohol reflux, extracting liquid filtering, united extraction liquid;
(4) vacuum concentration: extracting solution control vacuum tightness-more than the 0.80MPa, temperature obtains containing the one-level concentrated solution of alcohol concn about 5% at 50 ℃-70 ℃;
(5) deproteinated: the one-level concentrated solution adds CaCl 2Make the albumen rapid subsidence in ionogen, filter;
(6) absorption is resolved: the above-mentioned filtered liquid that obtains is transferred to macroporous resin column, and control absorption flow velocity washs decon with pure water then, resolves solvent with ethanol again and resolves, and obtains desorbed solution;
(7) secondary concentration: concentrate desorbed solution, obtaining desorbed solution, to contain alcohol concn be 2%, control vacuum tightness-more than the 0.80MPa, 50 ℃-70 ℃ of temperature obtain secondary concentration liquid;
(8) lyophilize: concentrated solution uses lyophilize, gets isoflavone genin.
The invention has the beneficial effects as follows: easy and simple to handle, the good reproducibility of production technique of the novel extraction isoflavone genin of this kind, the productive rate height, be applicable to suitability for industrialized production, adopt enzymolysis process to be converted into aglycon, the transformation efficiency height, technological process does not contain toxic solvent, and adopts ionogen sedimentation albumen, and dust removal rate is fast at a high speed.
Specific embodiment
The invention will be further described below by embodiment.
Embodiment 1: soyflour 500g, powder essence is to particle diameter 0.5mm-5.0mm, with twice of 3000ml 70% alcohol reflux, each 2 hours, united extraction liquid, vacuum concentration, control vacuum tightness-more than the 0.80MPa, temperature concentrates back control alcohol concn less than 5% below 70 ℃, and concentrated solution adds 0.5g CaCl 2Ionogen stirs, the protein deposition after-filtration, and filtrate being transferred to is equipped with the absorption of 500ml HPD100 type macroporous resin column, earlier with 1000ml pure water washing impurity, resolve with 1000ml 45% aqueous ethanolic solution again, the desorbed solution vacuum concentration, control vacuum tightness-more than the 0.80MPa, temperature is below 70 ℃, the about 100ml of concentrated solution volume, concentrated solution adds the 1g solid cellulase, stirs, controlled temperature 45-50 ℃, enzymolysis 48 hours adds ethyl acetate 50ml extraction, triplicate, the combined ethyl acetate extraction liquid, vacuum concentration, control vacuum tightness-more than the 0.80MPa, temperature is below 70 ℃, the concentrated solution lyophilize obtains the isoflavone genin of content about 40%.
Embodiment 2: soyflour 500g, powder essence is to particle diameter 0.5mm-5.0mm, with twice of 3000ml 80% alcohol reflux, each 3 hours, united extraction liquid, vacuum concentration, control vacuum tightness-more than the 0.80MPa, temperature concentrates back control alcohol concn less than 5% below 70 ℃, and concentrated solution adds 0.5g CaCl 2Ionogen stirs, the protein deposition after-filtration, filtrate being transferred to is equipped with the absorption of 500ml D101 type macroporous resin column, earlier with 1000ml pure water washing impurity, resolve with 1000ml 70% aqueous ethanolic solution again, the desorbed solution vacuum concentration, control vacuum tightness-more than the 0.80MPa, temperature is below 70 ℃, the about 100ml of concentrated solution volume.Concentrated solution adds the 1g solid cellulase, stirs, and controlled temperature 45-50 ℃, enzymolysis 36 hours, add ethyl acetate 50ml extraction, triplicate, combined ethyl acetate extraction liquid, vacuum concentration, control vacuum tightness-more than the 0.80MPa, temperature is below 70 ℃, the concentrated solution lyophilize.Obtain the isoflavone genin of content about 40%.

Claims (5)

1. the production technique of a novel extraction isoflavone genin is characterized in that:
(1) raw material is prepared: after soybean germ or bean cake powder essence to particle diameter are 0.5mm-5.0mm, standby;
(2) enzyme decomposes: add organized enzyme, controlled temperature is 40 ℃-50 ℃, and enzymolysis time is 24-72 hour;
(3) extract: solvent and raw material envelope-bulk to weight ratio are 5-10: 1, extract temperature at 70 ℃-100 ℃, and be the alcohol reflux twice of 60%-80% with concentration, united extraction liquid, and filter, get filtered liquid;
(4) vacuum concentration: extracting solution control vacuum tightness-more than the 0.80MPa, temperature obtains containing the one-level concentrated solution of alcohol concn about 5% at 50 ℃-70 ℃;
(5) deproteinated: the one-level concentrated solution adds CaCl 2Make the albumen rapid subsidence in ionogen, filter;
(6) absorption is resolved: the above-mentioned filtered liquid that obtains is transferred to macroporous resin column, and control absorption flow velocity washs decon with pure water then, resolves solvent with ethanol again and resolves, and obtains desorbed solution;
(7) secondary concentration: concentrate desorbed solution, obtaining desorbed solution, to contain alcohol concn be 2%, control vacuum tightness-more than the 0.80MPa, 50 ℃-70 ℃ of temperature obtain secondary concentration liquid;
(8) lyophilize: concentrated solution uses lyophilize, gets isoflavone genin.
2. the production technique of a kind of novel extraction isoflavone genin according to claim 1 is characterized in that: described enzyme is preferably used cellulase.
3. the production technique of a kind of novel extraction isoflavone genin according to claim 1 is characterized in that: described enzymolysis solution extraction organic solvent ethyl acetate.
4. the production technique of a kind of novel extraction isoflavone genin according to claim 1, it is characterized in that: described macroporous adsorbent resin can make D101, HPD100 and other polystyrene type macroporous adsorbent resins, resolves with the preferred 40-80% aqueous ethanolic solution of solvent.
5. the production technique of a kind of novel extraction isoflavone genin according to claim 1 is characterized in that: drying process is used lyophilize.
CN 201010294665 2010-09-27 2010-09-27 Novel production process for extracting soy isoflavones aglycones Pending CN101974577A (en)

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Application Number Priority Date Filing Date Title
CN 201010294665 CN101974577A (en) 2010-09-27 2010-09-27 Novel production process for extracting soy isoflavones aglycones

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102747116A (en) * 2012-06-12 2012-10-24 山东东兴生物科技股份有限公司 Production technology for extracting soybean isoflavone aglycone through enzymatic hydrolysis method
CN102994583A (en) * 2012-12-10 2013-03-27 天津大学 Production method of soybean isoflavone glycoside
CN105876751A (en) * 2016-05-03 2016-08-24 王胜 Plant salt containing soybean extract and preparation method of plant salt
CN106028795A (en) * 2013-12-13 2016-10-12 庆尚大学校产学协力团 Bean leaves or bean stems having high isoflavone derivative content, and method for preparing same
CN114214374A (en) * 2021-12-24 2022-03-22 四川宇奥生物科技有限公司 Method for preparing soybean isoflavone aglycone by fermentation method
CN114315782A (en) * 2021-12-24 2022-04-12 四川宇奥生物科技有限公司 Preparation method of soybean isoflavone

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1095468C (en) * 2000-08-04 2002-12-04 清华大学 Method of separating and refining soybean isoflavone from soybean dregs
CN1448394A (en) * 2002-04-01 2003-10-15 阎子鹏 Method for extracting soybean isoflavone from soya bean watse
CN1456559A (en) * 2002-05-10 2003-11-19 九台市东山粮库贸易部 Method for extracting soybean isoflavone
CN1483826A (en) * 2003-08-01 2004-03-24 金凤燮 Method for preparing aglycon of soybean isoflavone glycoside base by enzymatic method hydrolyzing soybean
CN1733926A (en) * 2004-08-13 2006-02-15 黑龙江双河松嫩大豆生物工程有限责任公司 Method for producing soybean isoflavones aglycone using glucosidase and membrane technology
CN101085768A (en) * 2007-07-11 2007-12-12 广东药学院 Method for preparing soybean isoflavone aglycon
CN101086002A (en) * 2007-06-22 2007-12-12 清华大学 Method for hydrolyzing soybean isoflavone by enzyme

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1095468C (en) * 2000-08-04 2002-12-04 清华大学 Method of separating and refining soybean isoflavone from soybean dregs
CN1448394A (en) * 2002-04-01 2003-10-15 阎子鹏 Method for extracting soybean isoflavone from soya bean watse
CN1456559A (en) * 2002-05-10 2003-11-19 九台市东山粮库贸易部 Method for extracting soybean isoflavone
CN1483826A (en) * 2003-08-01 2004-03-24 金凤燮 Method for preparing aglycon of soybean isoflavone glycoside base by enzymatic method hydrolyzing soybean
CN1733926A (en) * 2004-08-13 2006-02-15 黑龙江双河松嫩大豆生物工程有限责任公司 Method for producing soybean isoflavones aglycone using glucosidase and membrane technology
CN101086002A (en) * 2007-06-22 2007-12-12 清华大学 Method for hydrolyzing soybean isoflavone by enzyme
CN101085768A (en) * 2007-07-11 2007-12-12 广东药学院 Method for preparing soybean isoflavone aglycon

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102747116A (en) * 2012-06-12 2012-10-24 山东东兴生物科技股份有限公司 Production technology for extracting soybean isoflavone aglycone through enzymatic hydrolysis method
CN102994583A (en) * 2012-12-10 2013-03-27 天津大学 Production method of soybean isoflavone glycoside
CN106028795A (en) * 2013-12-13 2016-10-12 庆尚大学校产学协力团 Bean leaves or bean stems having high isoflavone derivative content, and method for preparing same
CN105876751A (en) * 2016-05-03 2016-08-24 王胜 Plant salt containing soybean extract and preparation method of plant salt
CN114214374A (en) * 2021-12-24 2022-03-22 四川宇奥生物科技有限公司 Method for preparing soybean isoflavone aglycone by fermentation method
CN114315782A (en) * 2021-12-24 2022-04-12 四川宇奥生物科技有限公司 Preparation method of soybean isoflavone

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Application publication date: 20110216