CN102742464B - Method for improving salt tolerance of dandelion by using photosynthetic bacteria - Google Patents
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a method for improving the salt tolerance of dandelion by using photosynthetic bacteria, and relates to a method for improving the adverse situation of soil. The method for improving the salt tolerance of the dandelion by using the photosynthetic bacteria is characterized by comprising the following steps of: before the dandelion is sowed, diluting photosynthetic bacteria stoste by 20 times, immersing the dandelion in the diluted stoste, and monitoring the chlorophyll content in the dandelion from the stage of one leaf and one heart, namely selecting 3 to 5 typical plants from a group body, completely unfolding the uppermost part of the leaf, measuring the chlorophyll content, if the chlorophyll content is 0.9 mg/L or so, not spraying the photosynthetic bacteria, or spraying a 0.1 mol/L calcium chloride solution to the surface of the leaf in a vigorous growth stage once, diluting the photosynthetic bacteria stoste by 200 times after 24 hours, and spraying the diluted photosynthetic bacteria stoste once. The method is easy to operate; phenomena of plant wilting, low growth speed and the like which are caused by high soil concentration can be effectively avoided; the plant growth is vigorous; and the flavonoid content is high.
Description
Technical field
The present invention relates to a kind of method of improving the soil adverse circumstance, particularly relate to a kind of method that photosynthetic bacteria is improved the dandelion salt resistance of using.
Background technology
Solonchak refers to that electrical conductivity in the soil saturation extract (ECe) is higher than 4ds/m, and the content of exchangeable sodium is lower than 15%, pH value and generally is no more than 8.5 soil.The whole world has 9.6 hundred million hectares of saline soil approximately, and the saline soil of China is also had an appointment 2,700 ten thousand hectares, and wherein having approximately 7,000,000 hectares is the farmland.The ionic species of salt damage mainly contains sodium ion, chlorion, calcium ion and sulfate ion etc., take chloride when main, salt content account for soil heavy 0.6% be solonchak.The anthropogenic soil salinization belongs to secondary saline soil more, the basic reason of solonchak low yield is that salt content is high, the osmotic pressure of the soil solution is higher than the osmotic pressure of plant tissue liquid, thereby plant is difficult to from soil to draw essential moisture and nutrient, causes occuring physiological drought, undergrowth even death.
To one of approach of saline soil development and use, be exactly the salt tolerant hereditary capacity of fully excavating and utilize plant, i.e. Selection of Salt-Tolerant kind and clone's salt resistant gene.Salt-tolerant plant has specific salt-resistance, has to secrete salt, rare salt, refuse varied accommodation modes to the salt marsh habitat such as salt, salt tolerant.Halophytes has kind more than 1560 approximately in the whole world, in state-owned 502 kinds, such as hair sweet potato, shrubby flase indigo, sweet clover, alkali cogongrass, Value of Spartina Anglica, bad grass, lyme grass, Siberian wheatgrass, witloof, Grain Production of Amaranthus, diversiform-leaved poplar, matrimony vine, arrow-leaved oleaster, Chinese tamarisk etc., this wherein has the crop of economic worth few.Mainly be because the secondary salinization that causes of unreasonable irrigation for the agricultural land soil salination, the characteristics that salt content is lower, be badly in need of obtaining to tolerate low concentration of salt soil and the crop of the not underproduction, to improve peasant's enthusiasm, if can process existing good crop varieties, making it to adapt to fully the solonchak environment, then has been the thing expected most.
Dandelion is the traditional Chinese medicine of multiple-effect commonly used, and nature and flavor are sweet flat, nontoxic, has broad-spectrum antibacterial and significantly bactericidal action, and the laudatory title of " natural antibiotics " is arranged; Dandelion is traditional nutrition and health care edible wild herbs, kept abundant nutrition and the unique local flavor of wild vegetable, with pollution-free green food and distinctive health-care effect liking of people extremely, and can go on the market before and after April 20, at this moment fresh leaf vegetables dish is seldom arranged on the market; Dandelion has the characteristic such as bloom the morning of turning green, withered and yellow evening, spring and autumn, makes of dandelion and sews flower lawn, more aobvious remarkable graceful bearing.Therefore, the market potential of dandelion is huge, and economic society is worth and can not despises.
In the cultivation, dandelion is the perennial root plant, and Second Year is blossomed and had seeds, and its adaptability is stronger, cold-resistant heat-resisting, more drought-resistant and sour, alkali if can further improve its salt resistance ability, then are expected to adapt to the soil types of higher salt concentrations, the agricultural land soil that is difficult to utilize for this class, even unreclaimed soil provides the feasible pattern of utilizing, and greatly increases the suitable kind of scope of dandelion, creates economy and social value.
Photosynthetic bacteria can carry out non-oxygen-production photosynthesis in the environment that the illumination anoxic is arranged, compare with higher plant, and it also contains and similarly catches photopigment, but only has a photosystem, i.e. PSI, and original hydrogen donor is not water, but H
2S (or some organic matters).Photosynthetic bacteria has been finished again and has produced very important chemical process in hydrogen, fixed nitrogen, three natural world material recycles of decomposing organic matter in the assimilation process of self.These features of photosynthetic bacteria have remedied the phenomenon that overground part photosynthesis that dandelion causes because of Root Absorption oligotrophy weakens in soil salt marsh adverse circumstance, greatly improve leaf chlorophyll and carotenoid content, strengthen photosynthetic rate, and promote conversely the growth of dandelion root system, thereby reach the raising resistance, the purpose that increases production and improves productivity.Therefore photosynthetic bacteria anaerobism illumination cultivation does not need general microbial inoculum to produce the required large-scale instrument and equipments such as fermentation tank, and production cost is very low, production process safety, environmental friendliness after using, efficient again, simple to operate, be to solve the first-selected way that salt marsh adverse circumstance soil is produced the dandelion problem.
Summary of the invention
The object of the present invention is to provide a kind of method that photosynthetic bacteria is improved the dandelion salt resistance of using.Foliage-spray photosynthetic bacteria microbial inoculum and calcium chloride (CaCl2) solution improves salt resistance, promotes Root growth, thereby reaches the raising resistance, dandelion growth in the salinization soil is had the purpose of efficient promotion.
The objective of the invention is to be achieved through the following technical solutions:
A kind of method of using photosynthetic bacteria raising dandelion salt resistance, described method comprises following process: before the dandelion sowing, get 20 times of seed soaking of photosynthetic bacteria microbial inoculum stoste dilution, from one heart stage an of leaf, monitor weekly the dandelion chlorophyll content, method is: select typical plant 3-5 strain in the colony, choose the fully expansion measuring blade chlorophyll content at place, topmost joint position, if chlorophyll content is when the 0.9mg/L left and right sides, can spray the photosynthetic bacteria microbial inoculum, or the calcium chloride solution foliage-spray of getting 0.1mol/L at the growth animated period once, gets 200 times of photosynthetic bacteria microbial inoculum stoste dilutions after 24 hours again and spray once and get final product.
The described a kind of method that photosynthetic bacteria is improved the dandelion salt resistance, its described photosynthetic bacteria microbial inoculum product characteristics: bronzing liquid used; Or faint yellow or yellowish-brown powder, component content: photosynthetic bacteria 〉=5,000,000,000/ml or photosynthetic bacteria 〉=10,000,000,000/gram.
Described a kind of method that photosynthetic bacteria is improved the dandelion salt resistance of using, its described photosynthetic bacteria microbial inoculum stoste: when product characteristics were liquid, product stoste was photosynthetic bacteria microbial inoculum stoste.When product characteristics are powder, in the ratio of 2ml distilled water microbial inoculum is made stoste according to every gram powder dissolution.
Described a kind of method that photosynthetic bacteria is improved the dandelion salt resistance of using, its described calcium chloride solution is: SILVER REAGENT, AR analyzes pure, is mixed with the calcium chloride solution that concentration is 0.1mol/L with distilled water.Advantage of the present invention and effect are:
Advantage of the present invention and effect are:
This method is easy and simple to handle, the operating period to accuse of characteristic index precisely clear and definite, use cost is low, production process safety, environmental friendliness after using has efficient promotion to dandelion growth in the salinization soil.Extremely significantly increase chlorophyll, carotenoid content, plant height and stem is thick, dry weight, fresh weight, photosynthesis all increase substantially, the resistance indexs such as soluble sugar, proline, beet alkali content improve, make dandelion can tolerate the salt-affected soil of 0.8-1.3%NaCl, effectively alleviate the plant wither that causes because of the soil concentration height, the phenomenons such as poor growth, plant strain growth is vigorous, and Flavonoid Content is high.
Embodiment
The present invention is described in detail below in conjunction with embodiment.
Use a kind of method that photosynthetic bacteria is improved the dandelion salt resistance.This method adopts photosynthetic bacteria and calcium chloride solution to process the dandelion plant to improve its salt resistance, take chlorophyll content in leaf blades as monitoring index to determine processing time and scheme.Photosynthetic bacteria component content 〉=5,000,000,000/ml or photosynthetic bacteria 〉=10,000,000,000/gram, calcium chloride solution concentration are 0.1mol/L.In the dandelion process of growth, if chlorophyll content about 0.9mg/L, can spray the photosynthetic bacteria microbial inoculum.If chlorophyll content is lower than 0.8mg/L or is lower than 0.6mg/L, get as early as possible the calcium chloride solution foliage-spray 1-2 time, get again 200 times of photosynthetic bacteria microbial inoculum stoste dilutions after 24 hours and spray, after interval 3-7 days, repeat spraying calcium chloride and photosynthetic bacteria microbial inoculum.
The acquisition of photosynthetic bacteria microbial inoculum: buy commercially available photosynthetic bacteria microbial inoculum.This product operative norm: in " People's Republic of China's agricultural industry criteria " " photosynthetic bacteria microbial inoculum NY 527-2002 ".Product characteristics: bronzing liquid; Faint yellow or yellowish-brown powder.Component content: photosynthetic bacteria 〉=5,000,000,000/ml or photosynthetic bacteria 〉=10,000,000,000/gram.
Photosynthetic bacteria microbial inoculum stoste: when product characteristics were liquid, product stoste was photosynthetic bacteria microbial inoculum stoste.When product characteristics are powder, in the ratio of 2ml distilled water microbial inoculum are made stoste according to every gram powder dissolution, as get the 250g powder, be dissolved in the 500ml distilled water, be photosynthetic bacteria microbial inoculum stoste.Calcium chloride (CaCl
2) solution: buy commercially available calcium chloride, SILVER REAGENT, AR analyzes pure getting final product.Be mixed with the calcium chloride solution that concentration is 0.1mol/L with distilled water.
Embodiment:
It is to improve resistance and the output of dandelion in the soil of 0.8-1.3%NaCl that this method is applicable in the secondary salinization level.
Before the dandelion sowing, get 20 times of seed soaking of photosynthetic bacteria microbial inoculum stoste dilution.From one heart stage an of leaf, monitor weekly the dandelion chlorophyll content.Method is: select typical plant 3-5 strain in the colony, choose the fully expansion blade at place, topmost joint position, according to Chen Jianxun (Chen Jianxun chief editor, " plant physiology experiment instructs (second edition) ", publishing house of South China Science ﹠ Engineering University, 87 pages) method measure chlorophyll content.If chlorophyll content is when the 0.9mg/L left and right sides, expression plant strain growth compared with normal this moment, can spray the photosynthetic bacteria microbial inoculum, or the calcium chloride solution foliage-spray of getting 0.1mol/L at the growth animated period once, get again 200 times of photosynthetic bacteria microbial inoculum stoste dilutions after 24 hours and spray once and get final product, grow promoting.
If chlorophyll content is lower than 0.8mg/L, the expression plant strain growth is subject to the impact of salt-affected soil, it is still normal that visually observe plant strain growth this moment, carefully identification can find that the plant under the leaf look compared with normal growing state is slightly light, should get as early as possible the calcium chloride solution foliage-spray of 0.1mol/L this moment, getting 200 times of photosynthetic bacteria microbial inoculum stoste dilutions after 24 hours sprays again, the interval is after one week, method repeat spraying calcium chloride as above and photosynthetic bacteria microbial inoculum, and continuous track determining chlorophyll content, until it reaches till the 0.9mg/L, collect seed as being used for, can be at initial bloom stage, the initial stage of setting seeds respectively sprays once.If chlorophyll content is lower than 0.6mg/L, the expression plant strain growth is had a strong impact on, and at first the leaf look thin out to visually observe plant, and withered phenomenon can appear in vane thickness attenuation subsequently, and lower blade is turned to be yellow even come off.Should before occuring, withered phenomenon spray as early as possible the calcium chloride solution of 0.1mol/L, the calcium chloride solution of foliage-spray 0.1mol/L again after 12 hours, to increase responsing reaction, getting 200 times of photosynthetic bacteria microbial inoculum stoste dilutions after 24 hours sprays again, behind the interval 3 days, repeat spraying calcium chloride and photosynthetic bacteria microbial inoculum.After spraying twice continuously, must stop using solvent more than 2 weeks, simultaneously continuous monitoring chlorophyll content according to new chlorophyll content numerical value behind the use solvent, is taked above-mentioned corresponding processing method.The management such as other liquid manure is normal.Other application notice of photosynthetic bacteria microbial inoculum please refer to its commodity operation instructions.
Claims (4)
1. use the method that photosynthetic bacteria is improved the dandelion salt resistance for one kind, it is characterized in that, described method comprises following process: before the dandelion sowing, get 20 times of seed soaking of photosynthetic bacteria microbial inoculum stoste dilution, from one heart stage an of leaf, monitor weekly the dandelion chlorophyll content, method is: select typical plant 3-5 strain in the colony, choose the fully expansion measuring blade chlorophyll content at place, topmost joint position, if chlorophyll content is when the 0.9mg/L left and right sides, needn't spray the photosynthetic bacteria microbial inoculum, or the calcium chloride solution foliage-spray of getting 0.1mol/L at the growth animated period once, get again 200 times of photosynthetic bacteria microbial inoculum stoste dilutions after 24 hours and spray once and get final product;
If chlorophyll content be lower than 0.8mg/L and 〉=0.6mg/L, should get as early as possible the calcium chloride solution foliage-spray of 0.1mol/L this moment, getting 200 times of photosynthetic bacteria microbial inoculum stoste dilutions after 24 hours sprays again, the calcium chloride solution foliage-spray of 0.1mol/L is got after one week in the interval, gets 200 times of photosynthetic bacteria microbial inoculum stoste dilutions after 24 hours again and sprays, and continuous track determining chlorophyll content, until it reaches till the 0.9mg/L, collect seed as being used for, respectively spray once at initial bloom stage, the initial stage of setting seeds;
If chlorophyll content is lower than 0.6mg/L, should before occuring, withered phenomenon spray as early as possible the calcium chloride solution of 0.1mol/L, the calcium chloride solution of foliage-spray 0.1mol/L again after 12 hours, to increase responsing reaction, getting 200 times of photosynthetic bacteria microbial inoculum stoste dilutions after 24 hours sprays again, behind the interval 3 days, repeat spraying calcium chloride and photosynthetic bacteria microbial inoculum; After spraying twice continuously, must stop using solvent more than 2 weeks, simultaneously continuous monitoring chlorophyll content according to new chlorophyll content numerical value behind the use solvent, is taked above-mentioned corresponding processing method.
2. a kind of method that photosynthetic bacteria is improved the dandelion salt resistance of using according to claim 1 is characterized in that described photosynthetic bacteria microbial inoculum product characteristics: bronzing liquid; Or faint yellow or yellowish-brown powder, component content: photosynthetic bacteria 〉=5,000,000,000/ml or photosynthetic bacteria 〉=10,000,000,000/gram.
3. a kind of method that photosynthetic bacteria is improved the dandelion salt resistance of using according to claim 2, it is characterized in that described photosynthetic bacteria microbial inoculum stoste: when product characteristics were liquid, product stoste was photosynthetic bacteria microbial inoculum stoste; When product characteristics are powder, in the ratio of 2ml distilled water microbial inoculum is made stoste according to every gram powder dissolution.
4. a kind of method that photosynthetic bacteria is improved the dandelion salt resistance of using according to claim 2, it is characterized in that described calcium chloride solution is: SILVER REAGENT, AR analyzes pure, is mixed with the calcium chloride solution that concentration is 0.1mol/L with distilled water.
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| CN101816253A (en) * | 2010-05-11 | 2010-09-01 | 沈阳化工学院 | Method for improving salt tolerance of dandelions by spraying microbes on leaf surfaces |
| CN101816252A (en) * | 2010-05-11 | 2010-09-01 | 沈阳化工学院 | Method for improving salt tolerance of cucumbers by spraying microbes on leaf surfaces |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101816253A (en) * | 2010-05-11 | 2010-09-01 | 沈阳化工学院 | Method for improving salt tolerance of dandelions by spraying microbes on leaf surfaces |
| CN101816252A (en) * | 2010-05-11 | 2010-09-01 | 沈阳化工学院 | Method for improving salt tolerance of cucumbers by spraying microbes on leaf surfaces |
| CN101836548A (en) * | 2010-05-11 | 2010-09-22 | 沈阳化工学院 | Method for improving salt tolerance of peppermint by spraying microbes to blade surfaces |
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| SHAO Shuang等.Effect of Inoculants Mixture on Nutrient Uptake and Growth in Cucumber(Cucumis sativus L.) Seedlings.《Agricultual Science&Technology》.2010,第11卷(第5期), |
| Technology》.2010,第11卷(第5期), * |
| 光合细菌促进黄瓜幼苗生长作用的研究;邵双等;《沈阳化工大学学报》;20100930;第24卷(第3期);第215-218页 * |
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| 邵双等.光合细菌促进黄瓜幼苗生长作用的研究.《沈阳化工大学学报》.2010,第24卷(第3期), |
| 陈华等.蒲公英研究进展和用生物技术培育耐盐蒲公英展望.《植物学通报》.2004,第21卷(第1期), |
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| CN102742464A (en) | 2012-10-24 |
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