CN102740700A - Mosquito repellent - Google Patents

Mosquito repellent Download PDF

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Publication number
CN102740700A
CN102740700A CN2010800394785A CN201080039478A CN102740700A CN 102740700 A CN102740700 A CN 102740700A CN 2010800394785 A CN2010800394785 A CN 2010800394785A CN 201080039478 A CN201080039478 A CN 201080039478A CN 102740700 A CN102740700 A CN 102740700A
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CN
China
Prior art keywords
bacillus
pathogenic bacteria
mosquito
mixture
holder
Prior art date
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Pending
Application number
CN2010800394785A
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Chinese (zh)
Inventor
鲁迪吉尔·克勒伊斯贝里斯
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Individual
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Individual
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Publication of CN102740700A publication Critical patent/CN102740700A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/10Animals; Substances produced thereby or obtained therefrom

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  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Pest Control & Pesticides (AREA)
  • Biotechnology (AREA)
  • Virology (AREA)
  • Agronomy & Crop Science (AREA)
  • Dentistry (AREA)
  • Wood Science & Technology (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Bedding Items (AREA)

Abstract

A mosquito repellent, consisting of a mixture of spores or living non-pathogenic bacteria, encapsulated or otherwise, applied to textiles, such as bedding, mattresses, sheets, pillows, textiles of sitting furniture or sleeping clothes, as well as human skin or animal fur or atomised by means of a dispenser, such as an atomiser, at the desired location.

Description

Culicifuge
Technical field
The present invention relates to be used for the composition of repel mosquitoes, it comprises the combination of non-pathogenic bacteria.
Particularly, the present invention relates to the composition of Bacillus (Bacillus genus) non-pathogenic bacteria, it is applied on the holder or through distributor and applies, and purpose is repel mosquitoes.Holder mainly is interpreted as and is meant that said composition can be enduringly or be applied on it any surface with the purpose that reaches repel mosquitoes occasionally.
Background technology
The mosquito that circles in the air mainly is great bothering in the bedroom.Mosquito bite is very irritating problem with corresponding itch.
Mosquito belongs to diptera (Diptera), specifically is Nematocera (Nematocera).
They are mainly breathed product C O 2Attract.
On the other hand, the heat of body and skin and moisture are important attraction sources for mosquito (Dulicidae).
Normally state of the art is known for culicifuge and utilization of pesticides (comprising those with effect exterminating mosquito).Under first kind of situation, tend to use odorant agent (odorous agents).
As culicifuge, DEET (N, the N-diethyl-' the m-toluamide) and the auspicious fourth of pick up (Picaridine) be well-known.
Using crude vegetal is known as culicifuge.
Lemongrass is a good instance.
In addition, eucalyptus citriodora oil or PMD (to terpane-3, the 8-glycol), cinnamon oil, rosemary oil and peppermint oil are included in the technology known in the art.
WO2002/002065 has described the insect repellent that is derived from fennel (Foeniculum Vulgare) fruit, uses (+)-fenchone and E-9-octadecenoic acid.
Using the shortcoming of mentioned reagent is that they need frequently apply, and smell possibly be fulsome.
And, be known that and use bacillus thuringiensis,Bt (Bacillus thuringiensis) bacterium (a kind of gram-positive spore forming bacteria), and contain various Israel subclass specifically with plasmid of effect exterminating mosquito.The Bacillus sphaericus subspecies also comprise the proteotoxin fatal to young mosquito (Toxicity of Bacillus sphaericus Crystal Toxin to Adult Mosquitoes; Stray ea.; Applied and Environmental Microbiology; Sept.1988, p.2320-2321).One of them instance is CrylAa.
GB2376887 has also described with driving insect oil and has used bacillus thuringiensis,Bt (Bti) to control mosquito.
WO01/91559 has described the formation as the desinsection companion spore albumin crystal of the bacillus thuringiensis,Bt of insect repellent.
The combination that the document is not described the kind of using Bacillus comes repel mosquitoes.And with wherein relate to as biocide and wherein said bacillus thuringiensis,Bt on the contrary by the US5352597 of starch encapsulated, bacillus thuringiensis,Bt is not sealed.
WO9628973 relates to the bioactivator sustained release of (being used to handle the bacillus thuringiensis,Bt mutation of aquatic organism).
JP6199616 has described and has adopted the insect composition that drives that does not have the Bacillus subtillis (B.subtilis) of sealing.
These documents do not have instruction to come from the live body repel mosquitoes through the clearly combination of using the spore of that provide and kind preferably put on the non-pathogenic Bacillus on the holder (for example live body) through sprayer in biopolymer that can be broken or synthetic capsule.
Summary of the invention
The objective of the invention is to comprise that through distributor the culicifuge of mixture of the non-pathogenic bacteria of Bacillus is applied on the holder or suitable place.Said non-pathogenic bacteria can apply with spore with the bacterium that lives.Mosquito is by from being applied to the non-pathogenic bacteria mixture that holder (for example fabric) is gone up or expelling through the place that distributor sprays the non-pathogenic bacteria mixture.
In addition, said holder can comprise application on human skin or animal fur, thus repel mosquitoes.First advantage of the present invention is if the mixture of the non-pathogenic bacteria that will exist with spore with the bacterium that lives is applied on the holder, can realize continuing longer effect.In addition, the prolificacy of bacterium is guaranteed long-term effect.
Because the mixture of non-pathogenic bacteria is tasteless, and do not comprise any harmful chemical, so it does not cause any other fulsome result, also do not stimulate air flue or skin, this is second advantage.
The non-pathogenic bacteria of Bacillus can be applied to multiple holder, on the for example different fabric fibres.This makes this mixture can be applied on bedding and clothing or the associated fabric.Mixture also can be applied on the fabric at seat of thing to sit on or means of transport, for example car seat or relevant seat cover.This is the 3rd advantage.
Embodiment
Below the present invention will be detailed.
Cube cage (the l:50cm of mouse is being housed; H:50cm and b:50cm) during the laboratory test of (holding more than 50 mosquito); We are surprised to find; Compare with the cage of a slice fabric of the mixture that comprises non-pathogenic bacteria, do not have mosquito (96.2% intrinsic fielding percentage (intrinsic defence)) to drop on the fabric (10 * 10cm is in the bottom of cage) that is applied with said mixture basically with Bacillus.The difference that drops between the mosquito number on the treated and undressed piece of cloth is called the intrinsic fielding percentage.
Find that also environment fielding percentage (ambient defence) (promptly dropping on the difference between the mosquito number on the target (=mouse) of representing treated and undressed piece of cloth) is 28.8%.These test triplicate.
The repeated test of in holding the closed area of mosquito, carrying out on people's forearm (cover and exposed forearm with fabric, and handle or handle) is illustrated in 5 hours the interior attack that average 10.8 mosquitoes are taken place of time period.When exposed forearm is handled with the emulsion of non-pathogenic bacteria, compare with undressed forearm, attack number of times and obviously reduce.Under the undressed situation, find 32 attacks, and under the treated situation, only after 3 hours, taking place for the first time, mosquito attacks.In addition, find to use the microcapsules that comprise non-pathogenic bacteria obviously to prolong and drive performance.Similar test shows that driveing effect continues 12 hours.
We reach a conclusion thus, and the non-pathogenic bacteria mixture of Bacillus has powerful repelling effect.
Especially, our mixture is made up of bacillus subtilis (Bacillus subtilis), bacillus amyloliquefaciens (Bacillus amyloliquefaciens), bacillus licheniformis (Bacillus licheniformis), bacillus pumilus (Bacillus pumilis) and bacillus megaterium (Bacillus megaterium).
What realize repelling effect mainly is the mutual and comprehensive effect of various bacteriums.
Depend on intended purposes, the non-pathogenic bacteria of Bacillus can be used as spore and applies as the bacterium that lives.Before under a kind of situation, be intended to obtain snap action (occasionally), under then a kind of situation, realize slowly many (continuing) but mainly be the long-term effect that continues.Use distributor, non-pathogenic bacteria is applied on precalculated position or the holder.Non-pathogenic bacteria can be used as oil emu or gel applies.
In another possible embodiment, live bacterium or its sporocyst are encapsulated in the capsule of being made up of biopolymer or synthetic polymer.With this form, can non-pathogenic bacteria be applied on the holder, and it will have slowly but the anti-mosquito effect of last much longer.
When the mixture of the non-pathogenic bacteria that applies Bacillus, can use distributor, atomizer for example, or they can apply through dipping or as the coating of interested holder.Fabric is interpreted as mainly being meant mattress, bedding, sheet, pillow, but also can refer to clothing and relative article, for example nightwear and handkerchief.Fabric can be the weaving or nonwoven.Fabric is handled with mixture so that mixture permeates the mode of fabric structure or fiber then.
And this mixture also can be applied on the fabric at thing to sit on (for example sofa in the living room or the seat in the means of transport) and on the relevant seat cover.
In addition, said mixture can be applied on application on human skin or the animal fur, thus repel mosquitoes.
In another possible embodiment, the non-pathogenic bacteria mixture of Bacillus is placed automatic distributor, it atomizes said mixture in the precalculated position.Distributor is interpreted as mainly being meant and is assigned in the air mixture or the atomizer on the fabric of pre-position.This automatics is equipped with pumping mechanism (pumping mechanism) usually.This device also can comprise timer, thereby at concrete time point aerosolized mixture.
This distributor is operated with electric power, battery or manual pumping mechanism.
If non-pathogenic bacteria is sealed, then the vibration of distributor can cause that the capsule that contains non-pathogenic bacteria is destroyed by internal friction power.These vibrations can be produced by ultrasonic.
This distributor must so that its position settle near the mode of potential target (for example human body, pet or fabric).

Claims (1)

1. be used to stop the composition of mosquito near live body; It comprises the combination of the non-pathogenic Bacillus bacterium that is selected from down group: bacillus subtilis, bacillus amyloliquefaciens, bacillus licheniformis, bacillus pumilus and bacillus megaterium; And the combination that it is characterized in that said Bacillus bacterium is a spore, its be encapsulated in can be broken capsule in and be applied on the holder.
Claim 1 is described to be used to stop the composition of mosquito near live body, it is characterized in that said holder comprises human body alive or animal body.
Claim 1 is described to be used to stop the composition of mosquito near live body, it is characterized in that said capsule comprises biopolymer or synthetic polymer.
Each describedly is used to stop the composition of mosquito near live body claim 1-3, it is characterized in that the combination of said non-pathogenic bacteria is applied on the said holder through watering can.
CN2010800394785A 2009-08-28 2010-08-27 Mosquito repellent Pending CN102740700A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
BE2009/0525 2009-08-28
BE2009/0525A BE1018872A5 (en) 2009-08-28 2009-08-28 PROTECTION AGAINST MUGS.
PCT/BE2010/000060 WO2011022790A2 (en) 2009-08-28 2010-08-27 Mosquito repellent

Publications (1)

Publication Number Publication Date
CN102740700A true CN102740700A (en) 2012-10-17

Family

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CN2010800394785A Pending CN102740700A (en) 2009-08-28 2010-08-27 Mosquito repellent

Country Status (4)

Country Link
EP (1) EP2470024A2 (en)
CN (1) CN102740700A (en)
BE (1) BE1018872A5 (en)
WO (1) WO2011022790A2 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107751256A (en) * 2017-11-08 2018-03-06 李倩 A kind of preparation method of long-acting mosquito-driving champignon liquid
CN108991040A (en) * 2018-07-11 2018-12-14 张培磊 A kind of efficient mosquito repelling champignon liquid and preparation method thereof

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20170196228A1 (en) * 2014-07-17 2017-07-13 Universiteit Gent Insect repellent
BE1023416B1 (en) 2016-02-26 2017-03-14 Chrisal Nv Synbiotic preparation

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH06199616A (en) * 1992-12-29 1994-07-19 Tsurumi Soda Co Ltd Insect pest repellent
WO2001091559A2 (en) * 2000-05-30 2001-12-06 Pieter Lodewikus Steyn Bacillus thuringiensis as insect repellent
EP1096959B1 (en) * 1998-07-13 2003-08-27 Life Science TGO, S.R.L. Bacteria as odor control agent for carpets
CN1491080A (en) * 2001-02-16 2004-04-21 �Ϻ���ͨ��ѧ Mixture of bacillus thuringiensis subspecies israelensis and bac illus sphaericus for management of resistance to mosquito larvicides
CN1515162A (en) * 2003-01-10 2004-07-28 深圳市水务(集团)有限公司 Preparation method of mosquito-killing preparation and its application
WO2006117019A1 (en) * 2005-04-29 2006-11-09 Westfaliasurge Gmbh Preparation and procedure for the treatment of udders
CN101347129A (en) * 2008-09-01 2009-01-21 佛山市正典生物技术有限公司 Bacillus thuringiensis suspending agent for killing mosquito

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5352597A (en) * 1993-11-15 1994-10-04 Industrial Technology Research Institute Method for the preparation of a biocidal formulation containing starch and Bacillus thuringiensis
US5698210A (en) * 1995-03-17 1997-12-16 Lee County Mosquito Control District Controlled delivery compositions and processes for treating organisms in a column of water or on land
FR2758051B1 (en) * 1997-01-06 1999-12-17 Cobiotex METHODS FOR SYSTEMATICALLY ERADICATING THE CARRYING OF PATHOGENS BY ANIMALS AND COMPOSITIONS USED THEREIN
KR100407605B1 (en) 2000-07-05 2003-12-01 주식회사 내츄로바이오텍 Repellent drug isolated from Foeniculum vulgare fruits
GB2376887B (en) 2001-04-04 2005-09-28 Gillian Roberts Mosquito control using surfactant which when applied to breeding ground acts a a powerful larvicide
JP4689092B2 (en) * 2001-06-29 2011-05-25 有限会社バイオフューチャー Deodorizing aerosol product and method for producing the same
US8025874B2 (en) * 2008-04-17 2011-09-27 Danisco A/S Bacillus strains useful for animal odor control

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH06199616A (en) * 1992-12-29 1994-07-19 Tsurumi Soda Co Ltd Insect pest repellent
EP1096959B1 (en) * 1998-07-13 2003-08-27 Life Science TGO, S.R.L. Bacteria as odor control agent for carpets
WO2001091559A2 (en) * 2000-05-30 2001-12-06 Pieter Lodewikus Steyn Bacillus thuringiensis as insect repellent
CN1491080A (en) * 2001-02-16 2004-04-21 �Ϻ���ͨ��ѧ Mixture of bacillus thuringiensis subspecies israelensis and bac illus sphaericus for management of resistance to mosquito larvicides
CN1515162A (en) * 2003-01-10 2004-07-28 深圳市水务(集团)有限公司 Preparation method of mosquito-killing preparation and its application
WO2006117019A1 (en) * 2005-04-29 2006-11-09 Westfaliasurge Gmbh Preparation and procedure for the treatment of udders
CN101347129A (en) * 2008-09-01 2009-01-21 佛山市正典生物技术有限公司 Bacillus thuringiensis suspending agent for killing mosquito

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107751256A (en) * 2017-11-08 2018-03-06 李倩 A kind of preparation method of long-acting mosquito-driving champignon liquid
CN108991040A (en) * 2018-07-11 2018-12-14 张培磊 A kind of efficient mosquito repelling champignon liquid and preparation method thereof

Also Published As

Publication number Publication date
BE1018872A5 (en) 2011-10-04
EP2470024A2 (en) 2012-07-04
WO2011022790A2 (en) 2011-03-03
WO2011022790A3 (en) 2011-10-27

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Application publication date: 20121017