CN102732471A - Bacillus subtilis and application thereof in preventing and treating plant leaf spot diseases - Google Patents
Bacillus subtilis and application thereof in preventing and treating plant leaf spot diseases Download PDFInfo
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- CN102732471A CN102732471A CN201210253953XA CN201210253953A CN102732471A CN 102732471 A CN102732471 A CN 102732471A CN 201210253953X A CN201210253953X A CN 201210253953XA CN 201210253953 A CN201210253953 A CN 201210253953A CN 102732471 A CN102732471 A CN 102732471A
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Abstract
The invention discloses bacillus subtilis which is classified and designated as bacillus subtilis JSNL1205 and preserved in the China General Microbiological Culture Collection center, the preservation number is CGMCC No.6303, and the preservation date is June, 28th, 2012. The invention also discloses application of the bacillus subtilis in preventing and treating plant leaf spot diseases such as dendrobium candidum. The bacillus subtilis JSNL1205 has a suppression effect on various pathogenic fungi on the dendrobium candidum, can be artificially cultured and has the advantages of high propagation speed, convenience for large-scale production and good development and application prospects.
Description
Technical field
The invention belongs to microbial technology field, be specifically related to a kind of subtilis and the application in control Herba Dendrobii leaf spot thereof.
Background technology
Biological pesticide (biopesticides) comprises botanical pesticide; Microbial pesticide; Mikrobe secondary metabolites (microbiotic) etc. are used to prevent and treat various insects and disease, endanger advantages such as little, safe in utilization because of it has; Become one of problem of being concerned about most in the agricultural sustainable development, become the research focus.Along with the common concern of the public, make full use of the useful microbe controlling plant diseases and come into one's own day by day chemical pesticide security in food and the environment.Especially got into since 21 century, the national governments and the common people pay attention to the development and application of biological pesticide more.China also classifies the development of microbial pesticide and exploitation as state key tackling of key scientific and technical problems and hi-tech development plan.The characteristic of biological pesticide has been given the agricultural sustainable development strategy, is the protection agroecological environment, and ensuring food safety provides material base and technical support; The biological pesticide industry also is one of new growth point that has in the future economy development potentiality simultaneously.
Herba Dendrobii (Dendrobium officinale Kimura et Migo) has another name called Herba hedyotis costatae; The orchid family Dendrobium plant; Being the perennial draft of growing nonparasitically upon another plant, is a kind of plant that integrates cultivation and view and admire, and is rare famous and precious Chinese medicinal materials; Classify top grade Chinese medicine as with quilts such as glossy ganoderma, genseng, entomophyte always, be described as first of " Chinese nine immortals grass ".Iron is green gains the name Herba Dendrobii because of epidermis is; Have nourishing Yin and clearing heat, nourishing the stomach to promote the production of body fluid, effect such as moisten the lung and relieve the cough; Its contained polysaccharide component has effects such as enhances human body immunologic function, cancer-resisting, recovery voice; Modern medicine proves, that Herba Dendrobii also has is antitumor, anti-ageing, enhancing body immunizing power and effectively alleviate effects such as liver injury.Because the environment of Herba Dendrobii happiness high humidity, the generation of leaf spot is comparatively serious, but because of medicinal characteristic, has determined can not use the control of its disease and pest the chemical pesticide of high poison.Therefore, seek a kind of economy, safe and effective prophylactico-therapeutic measures is the task of top priority, the biological control important supplementary means of can yet be regarded as, wherein biocontrol bacteria plays important effect in biocontrol of plant disease, and its main advantage is:
(1) kind and One's name is legion exist inside and outside plant materials in a large number;
(2) to the mode of action variation of pathogenic bacteria, comprise competition, antagonism, parasitism, inducing plant resistance generation etc.;
(3) reproduction speed is fast;
(4) bacterium colonazition inside and outside plant materials is strong mostly;
(5) can artificial culture, easy handling is convenient to production application;
(6) some bacterial isolates not only can be prevented and treated disease and can promote the growth of plant, increases the output of crop.
At present the control of Herba Dendrobii leaf spot is not still had ideal biological and ecological methods to prevent plant disease, pests, and erosion medicament,, will reduce pesticide residue to a certain extent, improve its edible safety greatly if the use agricultural chemicals that can use biological pesticide or try one's best few is is effectively prevented and treated the Herba Dendrobii leaf spot.From the angle of preserving the ecological environment with food safety, Herba Dendrobii production needs low toxicity, it is few to pollute, and the biotechnological formulation of human body safety is controlled disease, reaches the purpose of safe edible, and this also meets the strategy of sustainable development of agricultural.
Summary of the invention
Technical problem to be solved by this invention provides a kind of subtilis that Herba Dendrobii leaf spot pathogenic bacteria is had antagonistic action.
The technical problem that the present invention also will solve provides the application of above-mentioned subtilis in control Herba Dendrobii leaf spot.
For solving the problems of the technologies described above, the technical scheme that the present invention adopts is following:
The subtilis of high-efficiency prevention and control Herba Dendrobii leaf spot (Bacillus subtilis) the JSNL1205 rich garden of the farming Herba Dendrobii that is in October, 2010 in Jiangsu is planted and is separated acquisition in the district on the disease plant; This bacterial strain has significant antagonistic action to the Herba Dendrobii leaf spot; This bacterial strain has been preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center at present; Deposit number is CGMCC No.6303, and preservation date is on June 28th, 2012.The preservation address is No. 3 Institute of Microorganism, Academia Sinica in Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, postcode 100101.
The physiologically active characteristic of subtilis (Bacillus subtilis) JSNL1205 is following:
Subtilis (Bacillus subtilis) JSNL1205 bacterium colony on the beef-protein medium flat board is less, yellow, the general 3 ~ 5mm of diameter; Circle, neat in edge, smooth moistening; Produce the lavender pigment; Thalline is shaft-like, and size is 0.8 * (1 ~ 2) μ m, and one or three flagellums are given birth in the top.Gramstaining shows as feminine gender; Glucose oxidase fermentation reaction (O/F reaction) result produces acid, aerobic for oxidation; Oxydase reaction is the weak positive.
The application of above-mentioned subtilis in preventing and treating the leaf pinta, described plant are Herba Dendrobii, photinia glabra or strawberry.
JSNL1205 is inoculated in the fermention medium with subtilis (Bacillus subtilis), cultivates 48-72 hour at 28 ℃ of-35 ℃ of bottom fermentations, and the fermented liquid that obtains is as the microbial inoculum of control Herba Dendrobii leaf spot.
The prescription of fermention medium described in the present invention is: 1000mL zero(ppm) water, Carnis Bovis seu Bubali cream 2-5g, peptone 5-10g, sodium-chlor 4-5g, pH6.5-7.5.
Beneficial effect: compared with prior art, advantage of the present invention is:
(1) subtilis (Bacillus subtilis) JSNL1205 is inhibited to the various plants pathogenic bacteria; Play important effect at antagonistic bacterium in to biocontrol of plant disease; Be convenient to production application; Especially significant to the control of fungus-caused Herba Dendrobii leaf spot, also be the important means that guarantees edible safety.
(2) simple, the easy storing of subtilis (Bacillus subtilis) JSNL1205 culture condition is easy to suitability for industrialized production, has the excellent development application prospect.
Description of drawings
Fig. 1 is the indoor flat plate face-off effect of subtilis (Bacillus subtilis) JSNL1205 to Herba Dendrobii leaf spot pathogenic bacteria alternaria brassica (Alternaria brassicae).Wherein A is the indoor flat plate face-off figure of subtilis to pathogenic bacteria; B pathogenic bacteria single inoculation picture (CK), as follows.
Fig. 2 is the indoor flat plate face-off effect of subtilis (Bacillus subtilis) JSNL1205 to photinia glabra leaf spot pathogenic bacteria grape spore cup fungi (Botryotiniafuckeliana).
Fig. 3 subtilis (Bacillus subtilis) JSNL1205 is to the indoor flat plate face-off effect of the little spore plan of strawberry leaves pinta pathogenic bacteria dish stey (Pestalotiopsis microspora).
Embodiment
According to following embodiment, can understand the present invention better.Yet, those skilled in the art will readily understand that the described content of embodiment only is used to explain the present invention, and the present invention that should also can not limit in claims to be described in detail.
Embodiment 1:
Subtilis (Bacillus subtilis) JSNL1205 is to the dull and stereotyped bacteriostatic action of Herba Dendrobii leaf spot pathogenic bacteria alternaria brassica (Alternaria brassicae), photinia glabra leaf spot pathogenic bacteria grape spore cup fungi (Botryotinia fuckeliana), the little spore plan of strawberry leaves pinta pathogenic bacteria dish stey (Pestalotiopsis microspora).
To use subtilis (Bacillus subtilis) the JSNL1205 bacterial strain symmetry of LB slant activation to be inoculated in the dull and stereotyped both sides of PDA; Again the PDA plate culture of the pathogenic bacteria of Herba Dendrobii, photinia glabra and strawberry leaves pinta is beaten with the punch tool of diameter 5mm and got the bacterium cake of homogeneity, be inoculated in dull and stereotyped central authorities with amount.Cultivate for 27 ± 1 ℃ and observed fungistatic effect in 10 days, and Taking Pictures recording, experiment effect is shown in Fig. 1-Fig. 3, table 1.The result shows that subtilis Bacillus subtilisJSNL1205 all has tangible antagonistic action to 3 kinds of pathogenic bacterias.Dull and stereotyped with the PDA that singly connects pathogenic bacteria as contrast.
Table 1 JSNL1205 is to the inhibition effect of 3 kinds of pathogenic bacterias
Claims (3)
1. subtilis; Its classification called after subtilis (Bacillus subtilis) JSNL1205; Be preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center, deposit number is CGMCCNo.6303 at present, and preservation date is on June 28th, 2012.
2. the application of the described subtilis of claim 1 in preventing and treating the leaf pinta.
3. application according to claim 1 is characterized in that, described plant is Herba Dendrobii, photinia glabra or strawberry.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104041547A (en) * | 2014-05-29 | 2014-09-17 | 赵兰 | Natural bactericide for controlling strawberry leaf spot and preparation method thereof |
| CN104263673A (en) * | 2014-09-25 | 2015-01-07 | 江苏农林职业技术学院 | Bacillus subtilis B51 and application of bacillus subtilis B51 |
| CN104946553A (en) * | 2015-02-11 | 2015-09-30 | 江苏大学 | Bacillus subtilis strain and application thereof in resisting radix psuedostellariae leaf spot |
| CN105132294A (en) * | 2015-09-25 | 2015-12-09 | 江苏农林职业技术学院 | Alternaria alternata and application thereof to dendrobium officinale resistant to leaf spot |
| CN112998031A (en) * | 2021-02-02 | 2021-06-22 | 浙江大学 | Compound microbial agent for improving planting performance of dendrobium officinale and using method thereof |
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| CN101186887A (en) * | 2007-12-04 | 2008-05-28 | 华南农业大学 | Bacillus subtilis 203 for highly effective preventing and curing banana wilt and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101186887A (en) * | 2007-12-04 | 2008-05-28 | 华南农业大学 | Bacillus subtilis 203 for highly effective preventing and curing banana wilt and application thereof |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104041547A (en) * | 2014-05-29 | 2014-09-17 | 赵兰 | Natural bactericide for controlling strawberry leaf spot and preparation method thereof |
| CN104041547B (en) * | 2014-05-29 | 2015-11-18 | 赵兰 | A kind of natural bactericidal agent preventing and treating white leaf spot of strawberry and preparation method thereof |
| CN104263673A (en) * | 2014-09-25 | 2015-01-07 | 江苏农林职业技术学院 | Bacillus subtilis B51 and application of bacillus subtilis B51 |
| CN104946553A (en) * | 2015-02-11 | 2015-09-30 | 江苏大学 | Bacillus subtilis strain and application thereof in resisting radix psuedostellariae leaf spot |
| CN104946553B (en) * | 2015-02-11 | 2018-06-26 | 江苏大学 | A kind of bacillus subtilis and its application in anti-radix pseudostellariae leaf spot |
| CN105132294A (en) * | 2015-09-25 | 2015-12-09 | 江苏农林职业技术学院 | Alternaria alternata and application thereof to dendrobium officinale resistant to leaf spot |
| CN105132294B (en) * | 2015-09-25 | 2018-03-30 | 江苏农林职业技术学院 | A kind of alternaric bacteria and its application in dendrobium candidum disease-resistanting leaf spot |
| CN112998031A (en) * | 2021-02-02 | 2021-06-22 | 浙江大学 | Compound microbial agent for improving planting performance of dendrobium officinale and using method thereof |
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