CN104195072A - Bacillus amyloliquefaciens subsp.plantarum B232 and application thereof - Google Patents
Bacillus amyloliquefaciens subsp.plantarum B232 and application thereof Download PDFInfo
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Abstract
The invention discloses bacillus amyloliquefaciens subsp.plantarum which is classified and named as bacillus amyloliquefaciens subsp.plantarum B232 and preserved in the China General Microbiological Culture Collection Center (CGMCC) of the China Committee for Culture Collection of Microorganisms (CCCCM) on June 9th, 2014, and has the preservation number of CGMCCNo.9279. The bacillus amyloliquefaciens subsp.plantarum B232 is mainly used for carrying out biological prevention and treatment on Dothichiza populea Sacc.et Briard, and has good functions of inhibiting Dothichiza populea Sacc.et Briard germs, dendrobium officinale botrytis and other various pathogenic fungi. The bacillus amyloliquefaciens subsp.plantarum B232 taken as an antagonistic bacterium also has good effects of preventing and treating other plant diseases. The bacillus amyloliquefaciens subsp.plantarum B232 has the advantages of being rapid in propagation speed, easy to operate, convenient in production application, strong in stress resistance and the like, and can be artificially cultured. The bacillus amyloliquefaciens subsp.plantarum B232 requires a simple culture condition and is easy to store and favorable for industrial production. Thus, the bacillus amyloliquefaciens subsp.plantarum B232 has a good development and application prospect.
Description
Technical field
The invention belongs to plant protection field, relate in particular to a kind of bacillus amyloliquefaciens plant subspecies (Bacillus amyloliquefaciens subsp.plantarum) B232 and the application in the large patch canker disease of control willow thereof.
Background technology
Along with the common concern of the public to chemical pesticide security in environment, impel people to seek plant disease control new way safely and effectively, utilize nature beneficial microorganism controlling plant diseases increasingly to come into one's own.It is to introduce the external source Antagonistic Fungi to plant useful in the plant microecosystem being broken, by microecosystem regulation environment, impel natural beneficial microorganism colony constantly to breed, grow, and show biological and ecological methods to prevent plant disease, pests, and erosion activity, thereby reach the object that prevents Plant diseases.
The Microbial resources abundant species that nature is potential, aspect the product development of microbial pesticide, having a clear superiority in, therefore a large amount of manpower and financial resources is all dropped in countries in the world, research, exploitation have the novel microorganism agricultural chemicals of independent intellectual property right, and drive the development in an all-round way of other high-tech industries with this.China also classifies the development of microbial pesticide and exploitation as state key tackling of key scientific and technical problems and hi-tech development plan as, and Jiangsu Province government is an important component part using microbial pesticide initiative as biological husbantry also.
According to the definition of Copping and Menn, biological pesticide (biopesticides) has comprised living microorganism (fungi, bacterium and virus), botanical pesticide, secondary metabolites from microorganisms (microbiotic) etc., for preventing and treating each kind of plant insect and Plant diseases; The distinct advantages of microbial pesticide is relatively little to human health risk, safer to environment, one of this problem that whole society is concerned about most just.Therefore,, since entering 21 century, national governments and plant protection service person pay attention to the development and application of biological pesticide more, have become study hotspot.
The large patch canker disease of willow is caused by poplar ulcer shell spore (Dothichiza populea Sacc.et Br.), it is the new disease of a kind of destructiveness of willow, also be a kind of global Poplar Diseases, mainly occur in the main limb of willow, now classified China's forest plants quarantine disease as.Along with willow is grown seedlings and the increase of afforestation area, in the poplar forest on the ground such as Jiangsu Province, all have in various degree and occur, and be on the rise, but do not see the example that causes large tree dead.In area, Jurong main harm NL895 poplar.
From the safe angle of preserving the ecological environment, development is controlled Poplar Diseases to the safer microbial preparation of human body, meets biological husbantry development.
Summary of the invention
Goal of the invention: in order to overcome the deficiencies in the prior art, the first object of the present invention is to provide a kind of bacillus amyloliquefaciens plant subspecies (Bacillus amyloliquefaciens subsp.plantarum) B232 large patch canker germ of willow to antagonistic action.
The second object of the present invention is to provide the application of a kind of bacillus amyloliquefaciens plant subspecies (Bacillus amyloliquefaciens subsp.plantarum) B232 in the large patch canker disease of control willow.
Technical scheme: in order to solve above-mentioned the first object, the technical solution adopted in the present invention is: a kind of bacillus subtilis B 2 32, its Classification And Nomenclature is bacillus amyloliquefaciens plant subspecies (Bacillus amyloliquefaciens subsp.plantarum), (abbreviation CGMCC) preservation at China Committee for Culture Collection of Microorganisms's common micro-organisms center, depositary institution address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, postcode is 100101, its deposit number is: CGMCC9279, preservation date is on 06 09th, 2014.
In order to solve the second above-mentioned object, the technical solution adopted in the present invention is: the application of described bacillus amyloliquefaciens plant subspecies B232 in the large patch canker disease of control willow.
The application of described bacillus amyloliquefaciens plant subspecies B232 in suppressing the large patch canker pathogen growth of willow.
Have particular application as: withered bacillus amyloliquefaciens plant subspecies (Bacillus amyloliquefaciens subsp.plantarum) B232 is inoculated in fermention medium, at 28 ℃ of-35 ℃ of bottom fermentations, cultivate 48-72 hour, the fermented liquid obtaining is as the microbial inoculum of the large patch canker germ of control willow.
The formula of fermention medium described in the present invention is: 1000mL distilled water, extractum carnis 2-5g, peptone 5-10g, sodium-chlor 4-5g, pH6.5-7.5.
The application of described bacillus amyloliquefaciens plant subspecies B232 in suppressing the growth of Herba Dendrobii botrytis cinerea.
The application of described bacillus amyloliquefaciens plant subspecies B232 in suppressing Herba Dendrobii southern blight bacteria growing.
The application of described bacillus amyloliquefaciens plant subspecies B232 in suppressing Strawberry Root Rot pathogen growth.
The application of described bacillus amyloliquefaciens plant subspecies B232 in suppressing wheat scab bacteria growing.
The physiologically active feature of bacillus amyloliquefaciens plant subspecies described in the present invention (Bacillus amyloliquefaciens subsp.plantarum) B232 is as follows:
Described bacillus amyloliquefaciens plant subspecies (Bacillus amyloliquefaciens subsp.plantarum) B232 bacterium colony on beef-protein medium flat board is larger, oyster white, the general 4-6mm of diameter, surface elevation, dry, have gauffer, edge is more regular, and thalline is shaft-like, the blunt circle in two ends, tool peritrichous, size is 1.58-14.97 * 0.46-0.62 μ m, average 5.6 * 0.55 μ m; Gemma two ends are raw, ellipse, and size is 1.01-1.41 * 0.61-0.75 μ m, average 1.14 * 0.67 μ m.
Gramstaining shows as the positive; Casein hydrolysis, Starch Hydrolysis, gelatine liquefication are positive, and V.P reaction is variable, and indole reaction is negative; Carbohydrate acidogenic fermentation.
The 16SrDNA sequence of bacillus amyloliquefaciens is as shown in SEQ ID No.1.Surveyed 16SrDNA sequence is compared by BLAST, be accredited as bacillus amyloliquefaciens plant subspecies (Bacillus amyloliquefaciens subsp.plantarum).
Beneficial effect: compared with prior art, advantage of the present invention is:
(1) bacillus amyloliquefaciens plant subspecies (Bacillus amyloliquefaciens subsp.plantarum) B232 is inhibited to various plants pathogenic bacteria, at antagonistic bacterium, play very important effect in to biocontrol of plant disease, for example kind and One's name is legion; Mode of action variation to pathogenic bacteria; Reproduction speed is fast; Can artificial culture, easy handling, is convenient to production application; The advantages such as high-output stress-resistance, especially significant to the control of fungus-caused Herba Dendrobii root rot; Also be to guarantee Chinese medicine security-critical means.
(2) bacillus amyloliquefaciens plant subspecies (Bacillus amyloliquefaciens subsp.plantarum) B232 culture condition simply, is easily preserved, and is easy to suitability for industrialized production, has good development prospect.
Accompanying drawing explanation
The aspect graph of Fig. 1 a bacillus amyloliquefaciens plant subspecies (Bacillus amyloliquefaciens subsp.plantarum) B232;
Fig. 1 b is bacillus amyloliquefaciens plant subspecies (Bacillus amyloliquefaciens subsp. in the present invention
Plantarum) B232 gemma aspect graph (at one end or two ends refractive body)
Fig. 2 a is the restraining effect of bacillus amyloliquefaciens plant subspecies in the present invention (Bacillus amyloliquefaciens subsp.plantarum) B232 to the large patch canker germ of willow (Dothichiza populea Sacc.et Br.);
Fig. 2 b is the restraining effect of bacillus amyloliquefaciens plant subspecies in the present invention (Bacillus amyloliquefaciens subsp.plantarum) B232 to Herba Dendrobii botrytis cinerea (Botrytis cinerea) XC2;
Fig. 2 c is the restraining effect of (the Bacillus amyloliquefaciens subsp.plantarum) B232 of bacillus amyloliquefaciens plant subspecies in the present invention to Herba Dendrobii southern blight bacterium (Athelia rolfsii);
Fig. 2 d is the restraining effect of (the Bacillus amyloliquefaciens subsp.plantarum) B232 of bacillus amyloliquefaciens plant subspecies in the present invention to Strawberry Root Rot bacterium (Fusarium oxsporum);
Fig. 2 e is the restraining effect of (the Bacillus amyloliquefaciens subsp.plantarum) B232 of bacillus amyloliquefaciens plant subspecies in the present invention to fusarium graminearum (Fusarium asiaticum);
Fig. 3 a is the scab that bacillus amyloliquefaciens plant subspecies in the present invention (Bacillus amyloliquefaciens subsp.plantarum) B232 bacterium liquid is processed the large patch canker germ of inoculation willow;
Fig. 3 b does not use bacillus amyloliquefaciens plant subspecies (Bacillus amyloliquefaciens subsp.plantarum) B232 to process the inoculation of the large patch canker disease of willow in the present invention;
Fig. 3 c control treatment, wound heals.
Embodiment
Below in conjunction with experimental example, elaborate the application of bacillus amyloliquefaciens plant subspecies of the present invention (Bacillus amyloliquefaciens subsp.plantarum) B232.
Experimental example 1: the dull and stereotyped bacteriostatic action of bacillus amyloliquefaciens plant subspecies (Bacillus amyloliquefaciens subsp.plantarum) B232 to the large patch canker germ of willow (Dothichiza populea), Herba Dendrobii botrytis cinerea (Botrytis cinerea), Herba Dendrobii southern blight (Athelia rolfsii), Strawberry Root Rot (Fusarium oxsporum), fusarium graminearum (Fusarium asiaticum).
To use bacillus amyloliquefaciens plant subspecies (Bacillus amyloliquefaciens subsp.plantarum) the B232 bacterial strain streak inoculation of LB slant activation in the dull and stereotyped side of PDA, again the PDA plate culture of the large patch canker germ of willow, Herba Dendrobii botrytis cinerea, Herba Dendrobii southern blight bacterium, Strawberry Root Rot and fusarium graminearum 90mm is beaten and got homogeneity with the bacterium piece of amount with the punch tool of diameter 5mm, be inoculated in dull and stereotyped opposite side.Cultivate for 30 ± 1 ℃ and observe bacteriostasis in 14 days and 28 days, and record its width (as table 1), experiment effect is as shown in Fig. 2 a to Fig. 2 e.Test-results shows, bacillus amyloliquefaciens plant subspecies (Bacillus amyloliquefaciens subsp.plantarum) B232 all has good antagonistic action to 5 kinds of pathogenic bacterias, to occupy the diameter judgement of the culture dish effect of drawing up, with aseptic PDA nahlock (table 2) in contrast.
Bacillus amyloliquefaciens plant subspecies (Bacillus amyloliquefaciens subsp.plantarum) B232 cultivates after 28 days from different pathogenic fungies, and the inhibition zone between the two can diminish, the width different (table 2) of antagonism band.
The restraining effect of table 1 B232 to the large patch canker germ of willow and Herba Dendrobii grey mold pathogenic bacteria 14 days and 28 days
Note: the numeral in table 1 is to get the mean value of 5 PDA flat boards
1) restraining effect of bacillus amyloliquefaciens plant subspecies (Bacillus amyloliquefaciens subsp.plantarum) B232 to the large patch canker germ of willow (Dothichiza populea Sacc.et Br.), as shown in Figure 2 a;
2) restraining effect of bacillus amyloliquefaciens plant subspecies (Bacillus amyloliquefaciens subsp.plantarum) B232 to Herba Dendrobii botrytis cinerea (Botrytis cinerea) XC2, as shown in Figure 2 b;
3) restraining effect of bacillus amyloliquefaciens plant subspecies (Bacillus amyloliquefaciens subsp.plantarum) B232 to Herba Dendrobii southern blight bacterium (Athelia rolfsii), as shown in Figure 2 c;
4) restraining effect of bacillus amyloliquefaciens plant subspecies (Bacillus amyloliquefaciens subsp.plantarum) B232 to Strawberry Root Rot bacterium (Fusarium oxsporum), as shown in Figure 2 d;
5) restraining effect of bacillus amyloliquefaciens plant subspecies (Bacillus amyloliquefaciens subsp.plantarum) B232 to fusarium graminearum (Fusarium asiaticum), as shown in Figure 2 e;
Experimental example 2: bacillus amyloliquefaciens plant subspecies (Bacillus amyloliquefaciens subsp.plantarum) B232 meta-bolites is to the dull and stereotyped bacteriostatic action to the large patch canker germ of willow (Dothichiza populea), Herba Dendrobii botrytis cinerea (Botrytiscinerea), Herba Dendrobii southern blight (Athelia rolfsii), Strawberry Root Rot (Fusarium oxsporum), fusarium graminearum (Fusarium asiaticum).
The fermented liquid of cultivating bacillus amyloliquefaciens plant subspecies (the Bacillus amyloliquefaciens subsp.plantarum) B232 of 3 days filters with bacterial filter, and the filter membrane aperture of bacterial filter is 0.25 μ m; The 90mm PDA plate culture of 5 kind of plant pathogenic fungies is beaten and got homogeneity with the bacterium piece of amount with the punch tool of diameter 5mm, be inoculated in the dull and stereotyped side of PDA; Again the dull and stereotyped opposite side of PDA is beaten and got homogeneity with the nahlock of amount with the punch tool of diameter 5mm, and nahlock is taken out; The 100 μ L bacteria-free filtrates of the bacillus amyloliquefaciens plant subspecies B232 after filtering are connected in the dull and stereotyped opposite side of PDA cavity, 30 ± 1 ℃ of cultivations, respectively at the fungistatic effect of 14 days and 28 days observation bacillus amyloliquefaciens plant subspecies B232 again.Take that not meet bacillus amyloliquefaciens plant subspecies B232 be contrast, wait contrasting mycelia, cover with after flat board, observe antagonistic activity (in Table 2).Test-results shows, bacillus amyloliquefaciens plant subspecies B232 meta-bolites all has strong antagonistic action to 5 kinds of pathogenic bacterias.
Table 2 B232 fermented liquid presses down 5 kinds of large patch canker germs of willow and Herba Dendrobii ash arrhizus bacteria 14 days and 28 days
Make and use
Note: the numeral in table 1 is to get the mean value of 5 PDA flat boards
The restraining effect of Fig. 2 a bacillus amyloliquefaciens plant subspecies (Bacillus amyloliquefaciens subsp.plantarum) B232 to the large patch canker germ of willow (Dothichiza populea Sacc.et Br.);
The restraining effect of Fig. 2 b bacillus amyloliquefaciens plant subspecies (Bacillus amyloliquefaciens subsp.plantarum) B232 to Herba Dendrobii botrytis cinerea (Botrytis cinerea) XC2;
The restraining effect of Fig. 2 c bacillus amyloliquefaciens plant subspecies (Bacillus amyloliquefaciens subsp.plantarum) B232 to Herba Dendrobii southern blight bacterium (Athelia rolfsii);
The restraining effect of Fig. 2 d bacillus amyloliquefaciens plant subspecies (Bacillus amyloliquefaciens subsp.plantarum) B232 to Strawberry Root Rot bacterium (Fusarium oxsporum);
The restraining effect of Fig. 2 e bacillus amyloliquefaciens plant subspecies (Bacillus amyloliquefaciens subsp.plantarum) B232 to fusarium graminearum (Fusarium asiaticum);
Experimental example 3: whether checking bacillus amyloliquefaciens plant subspecies (Bacillus amyloliquefaciens subsp.plantarum) B232 has inhibiting test to the large patch canker germ of willow.
1, experimental installation
Bechtop, PRX-250A type intelligence growth cabinet, training English board DHZ-DA TCYQ constant temperature oscillator.
2, experimental principle
After willow, large patch canker germ is inoculated on willow trunk, bacillus amyloliquefaciens plant subspecies (Bacillus amyloliquefaciens subsp.plantarum) B232 nutrient solution brushing is surperficial at ulcer scab, B232 can invade continued growth in ulcer scab, breeding by the minute crack on wound and bark surface, produce meta-bolites, can effectively control the growth of the large patch canker germ of willow (Dothichiza populea).
3, experimental technique:
1) cultivation of bacillus amyloliquefaciens plant subspecies (Bacillus amyloliquefaciens subsp.plantarum) B232 bacterium liquid: bacillus amyloliquefaciens plant subspecies B232 is inoculated in the triangular flask that contains 300mL LB substratum, 120 turn, and cultivate 60 hours for 35 ℃.
2) sprinkling of B232 liquid fermentation agent control: in bacillus amyloliquefaciens plant subspecies (Bacillusamyloliquefaciens subsp.plantarum) B232, add 10 times of water, with atomizer, bacterium liquid is sprayed on the trunk of ulceration, after spraying time is preferably in 4 o'clock of afternoon.
3) contrast: process in contrast by the ulceration that sterilized water sprays on trunk.
4) antagonistic experiment: by step 2) with step 3) control experiment compare, and observations (as shown in Fig. 3 a to Fig. 3 c).
4, experimental technique:
1) bacillus amyloliquefaciens plant subspecies (Bacillus amyloliquefaciens subsp.plantarum) B232 can be good at preventing and treating willow top class in a kindergarten Peptic Ulcers, sees Fig. 3 a and 3b.Wherein, 3a is for being used the willow trunk of bacillus amyloliquefaciens plant subspecies (Bacillus amyloliquefaciens subsp.plantarum) B232 treatment Peptic Ulcers after 60 days; 3b is not for being used bacillus amyloliquefaciens plant subspecies (Bacillus amyloliquefaciens subsp.plantarum) B232 bacterium liquid to process the willow trunk of Peptic Ulcers.Bacillus amyloliquefaciens plant subspecies (Bacillus amyloliquefaciens subsp.plantarum) B232 has good prevention effect to the large patch canker disease of willow as can be seen here.
Specific embodiments of the invention below
Embodiment 1: the preparation method of bacillus amyloliquefaciens plant subspecies (Bacillus amyloliquefaciens subsp.plantarum) B232 microbial inoculum: bacillus amyloliquefaciens plant subspecies B232 is inoculated in fermention medium, pH6.5, in 500mL triangular flask, liquid amount is 300mL, 28 ℃-35 ℃, 120 revs/min of shaking speed, 48 hours, the fermented liquid obtaining was the original bacteria liquid of the large patch canker of control willow sick (Dothichiza populea).
Wherein the formula of fermention medium is: 1000mL distilled water, extractum carnis 2g, peptone 5g, sodium-chlor 5g, pH6.5.
Embodiment 2: the preparation method of bacillus amyloliquefaciens plant subspecies (Bacillus amyloliquefaciens subsp.plantarum) B232 microbial inoculum: bacillus amyloliquefaciens plant subspecies B232 is inoculated in fermention medium, pH7.5, in 500mL triangular flask, liquid amount is 300mL, 28 ℃-35 ℃, 160 revs/min of shaking speed, 72 hours, the fermented liquid obtaining was the original bacteria liquid of the large patch canker of control willow sick (Dothichiza populea).
Wherein the formula of fermention medium is: 1000mL distilled water, extractum carnis 5g, peptone 10g, sodium-chlor 5g, pH7.5.
Embodiment 3: the preparation method of bacillus amyloliquefaciens plant subspecies (Bacillus amyloliquefaciens subsp.plantarum) B232 microbial inoculum: bacillus amyloliquefaciens plant subspecies B232 is inoculated in fermention medium, pH7, in 500mL triangular flask, liquid amount is 300mL, 28 ℃-35 ℃, 150 revs/min of shaking speed, 55 hours, the fermented liquid obtaining was the original bacteria liquid of the large patch canker of control willow sick (Dothichiza populea).
Wherein the formula of fermention medium is: 1000mL distilled water, extractum carnis 3g, peptone 8g, sodium-chlor 5g, pH7.
Claims (9)
1. a bacillus amyloliquefaciens plant subspecies B232, its Classification And Nomenclature be bacillus amyloliquefaciens plant subspecies (
bacillus amyloliquefacienssubsp.
plantarum), being deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number is: CGMCC No.9279, preservation date is on 06 09th, 2014.
2. the application of bacillus amyloliquefaciens plant subspecies B232 claimed in claim 1 in the large patch canker disease of control willow.
3. the application of bacillus amyloliquefaciens plant subspecies B232 claimed in claim 1 in suppressing the large patch canker pathogen growth of willow.
4. application according to claim 2, is characterized in that, by withered bacillus amyloliquefaciens plant subspecies (
bacillus amyloliquefacienssubsp.
plantarum) B232 is inoculated in fermention medium, at 28 ℃ of-35 ℃ of bottom fermentations, cultivates 48-72 hour, the fermented liquid obtaining is as the microbial inoculum of the large patch canker germ of control willow.
5. application according to claim 3, is characterized in that, the formula of described fermention medium is: 1000 mL distilled water, extractum carnis 2-5g, peptone 5-10g, sodium-chlor 4-5g, pH6.5-7.5.
6. the application of bacillus amyloliquefaciens plant subspecies B232 claimed in claim 1 in suppressing the growth of Herba Dendrobii botrytis cinerea.
7. the application of bacillus amyloliquefaciens plant subspecies B232 claimed in claim 1 in suppressing Herba Dendrobii southern blight bacteria growing.
8. the application of bacillus amyloliquefaciens plant subspecies B232 claimed in claim 1 in suppressing Strawberry Root Rot pathogen growth.
9. the application of bacillus amyloliquefaciens plant subspecies B232 claimed in claim 1 in suppressing wheat scab bacteria growing.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105316264A (en) * | 2015-12-04 | 2016-02-10 | 北京农学院 | Bacillus amyloliquefaciens and application thereof |
| CN105316265A (en) * | 2015-12-04 | 2016-02-10 | 北京农学院 | Bacillus amyloliquefaciens used for biocontrol and application thereof |
| CN106479929A (en) * | 2016-10-27 | 2017-03-08 | 河北省微生物研究所 | Bacillus amyloliquefaciens and its application in the preventing and treating of numb Chinese yam root rot |
| WO2017039491A1 (en) * | 2015-09-02 | 2017-03-09 | Общество с ограниченной ответственностью "БИСОЛБИ ПЛЮС" | Bacterial strain bacillus amyloliquefaciens subsp. plantarum bs89 as agent for increasing productivity of plants and protecting same from diseases |
| RU2746928C2 (en) * | 2015-08-28 | 2021-04-22 | Агбайоми, Инк. | Bacterial strains and their use in plant disease control |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101967455A (en) * | 2009-12-31 | 2011-02-09 | 湖北省农业科学院植保土肥研究所 | Bacillus amyloliquefaciens EA19 for controlling wheat root diseases and preparation thereof |
| CN102604864A (en) * | 2012-03-09 | 2012-07-25 | 陕西加伦多作物科学有限公司 | Microbial bactericide and application thereof |
| CN102703354A (en) * | 2012-06-08 | 2012-10-03 | 江苏农林职业技术学院 | Bacillus amyloliquefaciens subsp. plantarum and application thereof |
| CN103289933A (en) * | 2013-06-06 | 2013-09-11 | 扬州大学 | Bacillus amyloliquefaciens plant subspecies B-01 and application thereof |
| CN103555607A (en) * | 2013-08-21 | 2014-02-05 | 青岛农业大学 | Separation method of endophyte H6 strain in Albizia julibrissin Durazz. blade, and application of endophyte H6 strain |
-
2014
- 2014-08-06 CN CN201410385641.3A patent/CN104195072A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101967455A (en) * | 2009-12-31 | 2011-02-09 | 湖北省农业科学院植保土肥研究所 | Bacillus amyloliquefaciens EA19 for controlling wheat root diseases and preparation thereof |
| CN102604864A (en) * | 2012-03-09 | 2012-07-25 | 陕西加伦多作物科学有限公司 | Microbial bactericide and application thereof |
| CN102703354A (en) * | 2012-06-08 | 2012-10-03 | 江苏农林职业技术学院 | Bacillus amyloliquefaciens subsp. plantarum and application thereof |
| CN103289933A (en) * | 2013-06-06 | 2013-09-11 | 扬州大学 | Bacillus amyloliquefaciens plant subspecies B-01 and application thereof |
| CN103555607A (en) * | 2013-08-21 | 2014-02-05 | 青岛农业大学 | Separation method of endophyte H6 strain in Albizia julibrissin Durazz. blade, and application of endophyte H6 strain |
Non-Patent Citations (2)
| Title |
|---|
| 冯志珍: "解淀粉芽孢杆菌FB_16的筛选及其对作物疫霉菌的抑制作用", 《中国优秀硕士学位论文全文数据库·农业科技辑》 * |
| 程少丽 等: "生防菌株B9601-Y2 对作物生长和产量的影响", 《长江蔬菜》 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2746928C2 (en) * | 2015-08-28 | 2021-04-22 | Агбайоми, Инк. | Bacterial strains and their use in plant disease control |
| WO2017039491A1 (en) * | 2015-09-02 | 2017-03-09 | Общество с ограниченной ответственностью "БИСОЛБИ ПЛЮС" | Bacterial strain bacillus amyloliquefaciens subsp. plantarum bs89 as agent for increasing productivity of plants and protecting same from diseases |
| CN105316264A (en) * | 2015-12-04 | 2016-02-10 | 北京农学院 | Bacillus amyloliquefaciens and application thereof |
| CN105316265A (en) * | 2015-12-04 | 2016-02-10 | 北京农学院 | Bacillus amyloliquefaciens used for biocontrol and application thereof |
| CN105316265B (en) * | 2015-12-04 | 2019-02-26 | 北京农学院 | One plant of biological and ecological methods to prevent plant disease, pests, and erosion bacillus amyloliquefaciens and its application |
| CN105316264B (en) * | 2015-12-04 | 2019-03-01 | 北京农学院 | One bacillus amyloliquefaciens and its application |
| CN106479929A (en) * | 2016-10-27 | 2017-03-08 | 河北省微生物研究所 | Bacillus amyloliquefaciens and its application in the preventing and treating of numb Chinese yam root rot |
| CN106479929B (en) * | 2016-10-27 | 2019-09-20 | 河北省微生物研究所 | Bacillus amyloliquefaciens and its application in the prevention and treatment of numb Chinese yam root rot |
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