CN103602606B - Bacillus amyloliquefaciens and application thereof in preventing and treating of plant fruit rot diseases - Google Patents
Bacillus amyloliquefaciens and application thereof in preventing and treating of plant fruit rot diseases Download PDFInfo
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- CN103602606B CN103602606B CN201310253591.9A CN201310253591A CN103602606B CN 103602606 B CN103602606 B CN 103602606B CN 201310253591 A CN201310253591 A CN 201310253591A CN 103602606 B CN103602606 B CN 103602606B
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Abstract
The invention discloses bacillus amyloliquefaciens and an application thereof in preventing and treating plant fruit rot, the classification name is bacillus amyloliquefaciens JSNL1312, the bacillus amyloliquefaciens JSNL1312 is preserved in the China General Microbiological Culture Collection Center, CGMCC, the preservation number is CGMCC No.7740, and the preservation date is June 13th, 2013. The invention also discloses an application of the acillus amyloliquefaciens JSNL1312 in preventing and treating fruit rot diseases of plants, such as ficus carica, dendrobium officinale, and the like. The bacillus amyloliquefaciens JSNL1312 has an inhibiting effect on a plurality of pathogenic fungi on fruits of ficus carica and dendrobium officinale, can be artificially cultured, has a fast breeding speed, is convenient to carry out massive production, and has a very good development and application prospect.
Description
Technical field
The invention belongs to microbial technology field, be specifically related to a kind of bacillus amyloliquefaciens and the application in control plant fruit rot thereof.
Background technology
Fructus Fici (Ficus carica Linn) belongs to Moraceae (Moraceae) Ficus (Ficus L.) plant, originate in the west and south, Asia and eastern region, Mediterranean Sea, it is ancient berry trees, meat is soft, local flavor is sweet, extensively plant in Mediterranean most countries and California now, there is very high nutritive value and pharmaceutical use.In recent years, the Fructus Fici produce market development in Jiangsu Province is very fast, and especially southern area of Jiangsu Province, supply falls short of demand for the products such as Ficus carica preserved fruits, fruit tea, fruit wine, and Fructus Fici industry faces the rare market opportunity.But fig fruit disease has become the important factor of restriction Fructus Fici postharvest storage phase and shelf-lives length, mainly comprise smut disease, black spot, Fructus Fici endosepsis and sour rot etc., not only affect the quality of fig fruit, and have a strong impact on Fructus Fici adopt after storage and sale.Wherein, the mould Fructus Fici endosepsis harm caused of epidemic disease is comparatively serious.
Herba Dendrobii (Dendrobium officinale Kimura et Migo) is perennial draft of growing nonparasitically upon another plant, it is a kind of plant integrating cultivation and view and admire, for rare famous and precious Chinese medicinal materials, be listed in top grade Chinese medicine with glossy ganoderma, ginseng, Cordyceps sinensis etc. always, be described as first of " Chinese nine immortal grass ".The effect such as Herba Dendrobii has nourishing Yin and clearing heat, nourishing the stomach to promote the production of body fluid, moisten the lung and relieve the cough, polysaccharide component contained by it has enhancing immune function of human body, modern medicine proves, Herba Dendrobii also has antitumor, anti-ageing, enhancing body immunizing power and effectively alleviates the effects such as liver injury.Due to the environment of Herba Dendrobii happiness high humidity, the generation of pod canker is comparatively serious, to its generation considerable influence that raises up seed.
Therefore, find a kind of economy, safe and effective prophylactico-therapeutic measures is the task of top priority, biological control be can yet be regarded as important supplementary means, wherein biocontrol bacteria plays very important effect in biocontrol of plant disease, there is the advantages such as harm is little, use safety, may be used for preventing and treating various Plant diseases, its main advantage is:
(1) kind and One's name is legion, exist in a large number inside and outside plant materials;
(2) to the mode of action variation of pathogenic bacteria, competition, antagonism, parasitism, inducing plant resistance generation etc. are comprised;
(3) reproduction speed is fast;
(4) bacterium colonazition inside and outside plant materials is strong mostly;
(5) can artificial culture, easy handling, is convenient to production application;
(6) some bacterial isolates can not only controlling disease and can promote the growth of plant, increases the output of crop.
At present ideal biological and ecological methods to prevent plant disease, pests, and erosion medicament be there is no to the control of various Plant diseases, if can use biological pesticide or as far as possible few use agricultural chemicals Plant diseases is is effectively prevented and treated, pesticide residue will be reduced to a certain extent, greatly improve its edible safety.From preserving the ecological environment and the angle of food safety, Herba Dendrobii, fig fruit are edible plants, and its production process needs low toxicity, low stain, and to reach the object of safe edible, this also meets the strategy of sustainable development of agricultural.Especially since entering 21 century, national governments and the common people pay attention to the development and application of biological pesticide more, and biological pesticide industry is considered to one of new growing point with development potentiality in the future economy.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of bacillus amyloliquefaciens Herba Dendrobii, Fructus Fici fruit rot to biological control effect.
The technical problem that the present invention also will solve is to provide the application of above-mentioned bacillus amyloliquefaciens in control Herba Dendrobii, Fructus Fici fruit rot.
For solving the problems of the technologies described above, the technical solution used in the present invention is as follows:
The bacillus amyloliquefaciens of high-efficiency prevention and control Herba Dendrobii, Fructus Fici fruit rot, its Classification And Nomenclature is bacillus amyloliquefaciens (Bacillus amyloliquefaciens) JSNL1312, plant in district at Jurong City of Jiangsu Province Fructus Fici in October, 2011 disease plant is separated acquisition, this bacterial strain has significant antagonistic action to Herba Dendrobii, Fructus Fici fruit rot, this bacterial strain has been preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center at present, deposit number is CGMCC No.7740, and preservation date is on June 13rd, 2013.Preservation address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City Institute of Microorganism, Academia Sinica, postcode 100101.
The physiologically active feature of bacillus amyloliquefaciens (Bacillus amyloliquefaciens) JSNL1312 is as follows:
Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) JSNL1312 bacterium colony on beef-protein medium flat board is less, faint yellow, general 3 ~ the 5mm of diameter, circle, edge is irregular, smooth moistening, produce faint yellow pigment, thalline is shaft-like, and size is 0.8 × (1 ~ 2) μm, top raw one or three flagellums.Gramstaining shows as feminine gender; Glucose oxidase fermentation reaction (O/F reaction) result is sour, aerobic for being oxidized product; Oxydase reaction is the weak positive.Its 16sRNA sequence is as shown in SEQ ID No:1.
The application of above-mentioned bacillus amyloliquefaciens in the corruption of control plant fruit, described plant is Herba Dendrobii or Fructus Fici.
Be inoculated in fermention medium by bacillus amyloliquefaciens (Bacillus amyloliquefaciens) JSNL1312, cultivate 48-60 hour at 28 DEG C of-35 DEG C of bottom fermentations, the fermented liquid obtained is as the microbial inoculum of control Herba Dendrobii, Fructus Fici fruit rot.
The formula of fermention medium described in the present invention is: 1000mL distilled water, extractum carnis 2-5g, peptone 5-10g, sodium-chlor 4-5g, pH6.5-8.0.
Beneficial effect: compared with prior art, advantage of the present invention is:
(1) bacillus amyloliquefaciens (Bacillus amyloliquefaciens) JSNL1312 is inhibited to various plants pathogenic bacteria, very important effect is played in antagonistic bacterium is to biocontrol of plant disease, be convenient to production application, especially significant to the control of fungus-caused Herba Dendrobii, Fructus Fici fruit rot, be also the important means ensureing edible safety.
(2) bacillus amyloliquefaciens (Bacillus amyloliquefaciens) JSNL1312 culture condition simply, is easily preserved, and is easy to suitability for industrialized production, has good development prospect.
Accompanying drawing explanation
Fig. 1 is the indoor flat plate face-off effect of bacillus amyloliquefaciens (Bacillus amyloliquefaciens) JSNL1312 to Fructus Fici fruit rot pathogenic bacteria Fusarium equiseti (Fusarium equiseti).Wherein A is the indoor flat plate face-off figure of bacillus amyloliquefaciens to pathogenic bacteria; B pathogenic bacteria single inoculation picture (CK), as follows.
Fig. 2 is the indoor flat plate face-off effect of bacillus amyloliquefaciens (Bacillus amyloliquefaciens) JSNL1312 to burnt maize ear rot bacterium (Lasiodiplodia theobromae) of Herba Dendrobii fruit rot pathogenic bacteria longan.
Embodiment
According to following embodiment, the present invention may be better understood.But those skilled in the art will readily understand, the content described by embodiment only for illustration of the present invention, and should can not limit the present invention described in detail in claims yet.
Embodiment 1:
Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) JSNL1312 is to the dull and stereotyped bacteriostatic action of Herba Dendrobii, Fructus Fici fruit rot pathogenic bacteria.
Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) JSNL1312 bacterial strain symmetry by LB slant activation is inoculated in the both sides of PDA flat board, again the punch tool of the PDA plate culture diameter 5mm of Herba Dendrobii, Fructus Fici fruit rot pathogenic bacteria is beaten and get the bacterium cake of homogeneity with amount, be inoculated in dull and stereotyped central authorities.Cultivate for 27 ± 1 DEG C and observe fungistatic effect in 10 days, and Taking Pictures recording, experiment effect is as shown in Fig. 1-Fig. 2, table 1.Result shows, bacillus amyloliquefaciens Bacillusamyloliquefaciens JSNL1312 all has obvious antagonistic action to 2 kinds of pathogenic bacterias.With singly connecing the PDA flat board of pathogenic bacteria in contrast.
Table 1JSNL1312 is to the inhibition of 2 kinds of pathogenic bacterias
Claims (3)
1. the application of bacillus amyloliquefaciens in control plant fruit rot, described plant is Herba Dendrobii or Fructus Fici; Described bacillus amyloliquefaciens, its Classification And Nomenclature is bacillus amyloliquefaciens (Bacillusamyloliquefaciens) JSNL1312, now be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number is CGMCC No.7740, and preservation date is on June 13rd, 2013.
2. application according to claim 1, it is characterized in that, bacillus amyloliquefaciens (Bacillusamyloliquefaciens) JSNL1312 is inoculated in fermention medium, cultivate 48-60 hour at 28 DEG C of-35 DEG C of bottom fermentations, the fermented liquid obtained is as the microbial inoculum of control Herba Dendrobii, Fructus Fici fruit rot.
3. application according to claim 2, is characterized in that, the formula of described fermention medium is: 1000mL distilled water, extractum carnis 2-5g, peptone 5-10g, sodium-chlor 4-5g, pH6.5-8.0.
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| CN104152382B (en) * | 2014-08-08 | 2017-09-22 | 中国农业大学 | One bacillus amyloliquefaciens and its application |
| CN104560837B (en) * | 2015-02-02 | 2017-10-03 | 青岛蔚蓝生物集团有限公司 | A kind of bacillus amyloliquefaciens and its application |
| CN107629985B (en) * | 2017-11-01 | 2020-09-04 | 广州市林业和园林科学研究院 | Plant endophytic bacterium with antagonistic effect on plant pathogenic fungi |
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