CN102731513B - Method for extracting sodium copper chlorophyllin from wild deyeuxia angustifolia forage grass - Google Patents
Method for extracting sodium copper chlorophyllin from wild deyeuxia angustifolia forage grass Download PDFInfo
- Publication number
- CN102731513B CN102731513B CN201210144911.2A CN201210144911A CN102731513B CN 102731513 B CN102731513 B CN 102731513B CN 201210144911 A CN201210144911 A CN 201210144911A CN 102731513 B CN102731513 B CN 102731513B
- Authority
- CN
- China
- Prior art keywords
- chlorophyll
- copper
- extraction
- saponification
- filtrate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Peptides Or Proteins (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention belongs to the field of natural organic chemistry, and relates to a method for extracting chlorophyll from wild deyeuxia angustifolia forage grass and converting the chlorophyll into sodium copper chlorophyllin, and particularly to a new method, wherein the new method comprises adopting a two-step method to extract chlorophyll and grass protein, carrying out alkaline solution saponification on the paste chlorophyll, and replacing copper with sodium copper chlorophyllin. The method has the following advantages that: 1, the two-step method is adopted to extract chlorophyll, such that the time is shortened compared with the conventional method, and the active ingredient chlorophyll extraction rate is improved; 2, wild forage grass deyeuxia angustifolia in Daxinganling is adopted as the raw material, such that the local wild resources are comprehensively utilized, and two substances of grass protein and sodium copper chlorophyllin can be obtained; and 3, the method of the present invention has characteristics of simpleness, easy operation, simple process, low investment, and low production cost, and is suitable for large-scale production. With the method of the present invention, the two-step extraction method is adopted to extract chlorophyll, the grass is grinded and extracted, the filter residue is soaked and extracted to obtain the glass protein and the paste chlorophyll, alkaline solution saponification is performed, acidification is performed after extraction is performed, copper is replaced, and purifying is performed to obtain the sodium copper chlorophyllin.
Description
Technical field:
The invention belongs to natural organic chemistry field, relate to and a kind ofly from wild leaflet camphor herbage, extract the method that chlorophyll changes into sodium copper chlorophyllin, particularly relate to a kind of two-step approach extracting chlorophyll and vegetable protein of utilizing, chlorophyll paste is through alkali lye saponification, and copper is replaced as the novel method of sodium copper chlorophyllin.
Background technology:
Daxing'an Mountainrange leaflet camphor grass, also claims to cover room grass with mat, is the leafy fast-growing grass class of thin stem, general grows wild, and in forest zone, by the ditch pool on ground, be low-lyingly located in growth, very luxuriant, some places are grown in flakes, and the same like the wheatland of plantation, output is very high.According to surveying and determination, leaflet camphor more than 30%, is the good feed of milk cow containing crude protein 10%～16%, crude fat 1%～2%, nitrogen-free extract.Leaflet camphor chlorophyll is water insoluble, is dissolved in organic solvent, and available multiple organic solvent, as extractions such as acetone, ethanol or dimethyl sulfoxide (DMSO).The multiplex silkworm faeces of domestic chlorophyll copper sodium extracting, the leaf of bamboo, Leaf of Japanese Banana are made raw material, and with Daxing'an Mountainrange fresh wild herbage leaflet camphor grass, make the report that has no of raw material.Therefore, meaning of the present invention is not only to fully utilize local wild resource, exploitation food dye new variety, and to the reference frame that made full use of similar sylvan life Resource Supply.
Summary of the invention:
The present invention adopts two-step approach extracting chlorophyll, blade of grass milling and extracting, and filter residue soaks and extracts, and obtains vegetable protein and chlorophyll paste, then through alkali lye saponification, after extraction, copper is put in acidifying, purifying becomes sodium copper chlorophyllin.The present invention is raw materials used is that Daxing'an Mountainrange is wild, without worrying raw material supply problem
Invention advantage is as follows:
1, the present invention adopts two-step approach extracting chlorophyll, and chlorophyll effective component extraction rate is high.
2, adopt Daxing'an Mountainrange Wild Grass leaflet camphor to make raw material, fully utilize local wild resource, can obtain two kinds of materials of vegetable protein and sodium copper chlorophyllin.
3, the inventive method is simple to operation, and technique is simple, and be applicable to people and produce, less investment, production cost is low.
For achieving the above object, the technical solution used in the present invention is: a kind of two-step approach extracting chlorophyll and vegetable protein of utilizing, and chlorophyll paste is through alkali lye saponification, and copper is replaced as the novel method of sodium copper chlorophyllin.Its step is as follows:
(1) chlorophyll is just carried:
Fresh leaflet camphor grass cuts off 3-5cm, and 6-8 times of 80-90% ethanol extracts, and adds mortar to grind to form homogenate, standing, filters to get filtrate, and uses alcohol flushing mortar, and filtrate merges;
(2) filter residue second extraction:
Filter residue, through squeezing machine, must be remained to fresh juice, filter, after all filtrate is merged, add gasoline, fully stir, be placed in respectively separating funnel, more than sufficient standing 2-3h, liquid is divided into three layers.
(3) obtain primary extract
Upper strata is oil reservoir, is blackish green, and lower floor is that yellow liquid claims water layer, and middle level is suspended on water layer, under upper strata, claims the greyish white beds of precipitation.Water layer, the greyish white beds of precipitation, oil reservoir are filtered respectively, and filter residue merges, and with distilled water wash, drains, and in 90-100 ℃ of oven dry, obtains vegetable protein; By oil reservoir decompression recycling ethanol and gasoline, obtain chlorophyll paste;
(4) saponification: with the leaf of 4 volumes, practice the sodium hydroxide solution that vegetarian feast seminal fluid adds the 5%-10% of 1 volume and carry out saponification, temperature 50-60 ℃, saponification 1h, stratification, lower floor is water capacity chlorophyll saponification liquor;
(5) extraction:
By chlorophyll saponification liquor petroleum ether extraction oil-soluble impurities, extraction secondary adds in equivalent sherwood oil fourth alcohol saponification liquor at every turn, stirs stratification, emits petroleum ether layer, obtains extraction liquid;
(6) put copper
Extraction liquid regulates pH value to subacidity, adds 20% copper sulfate solution, then is adjusted to pH value 2-3, keeps 30-60min at 60 ℃, fully puts copper.
(7) purifying:
Filter, by 90% washing with alcohol 1-2 time, washings merges with filtrate, and the water that doubles dilutes, and chlorophyll cupric acid can be separated out, and dries that to obtain the green copper of solid leaf sour, uses the Diluted Alcohol solution washing of 40-50% several times, is filtered dry and gets final product purifying.
(8) salify;
Add 5% sodium hydroxide solution, dissolved copper acid, is added to PH always and is till 11, and salification process is first early thick, with sodium hydroxide, adds, gradually thinning,, until during PH 11, retrogradation again again.After salify, filter, filter residue distilled water flushing 1-2 time, washings is incorporated in filtrate, 60-80 ℃ of vacuum hydro-extraction, obtains finished product.
Embodiment:
Embodiment 1: a kind of two-step approach extracting chlorophyll and vegetable protein of utilizing, and chlorophyll paste is through alkali lye saponification, and copper is replaced as the novel method of sodium copper chlorophyllin.Its step is as follows;
(1) fresh leaflet camphor grass 1000g cuts off 3cm, and 6 times of 80% ethanol extracts, and adds mortar to grind to form homogenate, standing, filters to get filtrate, and uses alcohol flushing mortar;
(2) by filter residue process squeezing machine, must remain fresh juice, filter, will after all filtrate merging, add gasoline, fully stir, be placed in respectively separating funnel, more than sufficient standing 2h, liquid is divided into three layers.
(3) upper strata is oil reservoir, is blackish green, and lower floor is that yellow liquid claims water layer, and middle level is suspended on water layer, under upper strata, claims the greyish white beds of precipitation.Water layer, the greyish white beds of precipitation, oil reservoir are filtered respectively, and filter residue merges, and with distilled water washing, drains, and in 90 ℃ of oven dry, obtains vegetable protein 82g; By oil reservoir decompression recycling ethanol and gasoline, obtain chlorophyll paste 5.89g;
(4) with 8% the sodium hydroxide solution that the chlorophyll alcohol liquid of 4 volumes adds 1 volume, carry out saponification, 55 ℃ of temperature, saponification 1h, stratification, lower floor is chlorophillins saponification liquor;
(5), by chlorophyll saponification liquor petroleum ether extraction oil-soluble impurities, extraction secondary adds equivalent sherwood oil in alcohol saponification liquor at every turn, stirs stratification, emits petroleum ether layer, obtains extraction liquid;
(6) extraction liquid regulates pH value to subacidity, adds 20% copper sulfate solution, then is adjusted at 2,60 ℃ of pH values and keeps 30min, fully puts copper.
(7) filter, with 90% washing with alcohol 1-2 time, washings and filtrate merging, the water that doubles dilutes, and chlorophyll cupric acid can be separated out, and dries that to obtain the green copper of solid leaf sour, and the Diluted Alcohol solution washing with 40% several times, is filtered dry and gets final product purifying.
(8) add 5% sodium hydroxide solution, dissolved copper acid, is added to PH always and is till 11, and salification process is first early thick, with sodium hydroxide, adds, gradually thinning,, until during PH 11, retrogradation again again.After salify, filter, filter residue distilled water flushing 1-2 time, washings is incorporated in filtrate, at 60 ℃, dries to obtain finished product 5.73g.
Case study on implementation 2:
(1) fresh leaflet camphor grass 1000g, cuts off 4cm, and 7 times of 85% ethanol extracts, and adds mortar to grind to form homogenate, standing, filters to get filtrate, and uses alcohol flushing mortar;
(2) by filter residue process squeezing machine, must remain fresh juice, filter, will after all filtrate merging, add gasoline, fully stir, be placed in respectively separating funnel, more than sufficient standing 3h, liquid is divided into three layers.
(3) upper strata is oil reservoir, is blackish green, and lower floor is that yellow liquid claims water layer, and middle level is suspended on water layer, under upper strata, claims the greyish white beds of precipitation.Water layer, the greyish white beds of precipitation, oil reservoir are filtered respectively, and filter residue merges, and with distilled water wash, drains, and in 90 ℃ of oven dry, obtains vegetable protein 83.7g; By oil reservoir decompression recycling ethanol and gasoline, obtain chlorophyll paste 5.93g;
(4) with 10% the sodium hydroxide solution that the chlorophyll alcohol liquid of 4 volumes adds 1 volume, carry out saponification, temperature 60 C, saponification 1h, stratification, lower floor is chlorophillins saponification liquor;
(5), by chlorophyll saponification liquor petroleum ether extraction oil-soluble impurities, extraction secondary adds equivalent sherwood oil in alcohol saponification liquor at every turn, stirs stratification, emits petroleum ether layer, obtains extraction liquid;
(6) extraction liquid regulates pH value to subacidity, adds 20% copper sulfate solution, then is adjusted at 3,60 ℃ of pH values and keeps 40min, fully puts copper.
(7) filter, with 90% washing with alcohol 1-2 time, washings and filtrate merging, the water that doubles dilutes, and chlorophyll cupric acid can be separated out, and dries that to obtain the green copper of solid leaf sour, and the Diluted Alcohol solution washing with 50% several times, is filtered dry and gets final product purifying
(8) add 5% sodium hydroxide solution, dissolved copper acid, is added to PH always and is till 11, and salification process is first early thick, with sodium hydroxide, adds, gradually thinning,, until during PH 11, retrogradation again again.After salify, filter, filter residue distilled water flushing 1-2 time, washings is incorporated in filtrate, at 80 ℃, dries to obtain finished product 5.78g.
Case study on implementation 3:
(1) fresh leaflet camphor grass 1000g, cuts off 5cm, and 8 times of 90% ethanol extracts, and adds mortar to grind to form homogenate, standing, filters to get filtrate, and uses alcohol flushing mortar;
(2) by filter residue process squeezing machine, must remain fresh juice, filter, will after all filtrate merging, add gasoline, fully stir, be placed in respectively separating funnel, more than sufficient standing 3h, liquid is divided into two layers.
(3) upper strata is oil reservoir, is blackish green, and lower floor is that yellow liquid claims water layer, and middle level is suspended on water layer, under upper strata, claims the greyish white beds of precipitation.Water layer, the greyish white beds of precipitation, oil reservoir are filtered respectively, and filter residue merges, and with distilled water wash, drains, and in 100 ℃ of oven dry, obtains vegetable protein 81.2g; By oil reservoir decompression recycling ethanol and gasoline, obtain chlorophyll paste 5.88g;
(4) with 5% the sodium hydroxide solution that the chlorophyll alcohol liquid of 4 volumes adds 1 volume, carry out saponification, temperature 50 C, saponification 1h, stratification, lower floor is chlorophillins saponification liquor;
(5), by chlorophyll saponification liquor petroleum ether extraction oil-soluble impurities, extraction secondary adds equivalent sherwood oil in alcohol saponification liquor at every turn, stirs stratification, emits petroleum ether layer, obtains extraction liquid;
(6) extraction liquid regulates pH value to subacidity, adds 20% copper sulfate solution, then is adjusted to pH value 2-5, keeps 50min at 60 ℃, fully puts copper.
(7) filter, with 90% washing with alcohol 1-2 time, washings and filtrate merging, the water that doubles dilutes, and chlorophyll cupric acid can be separated out, and dries to obtain the green copper acid 5.758 of solid leaf, and the Diluted Alcohol solution washing with 50% several times, is filtered dry and gets final product purifying.
(8) add 5% sodium hydroxide solution, dissolved copper acid, is added to PH always and is till 11, and salification process is first early thick, with sodium hydroxide, adds, gradually thinning,, until during PH 11, retrogradation again again.After salify, filter, filter residue distilled water flushing 1-2 time, washings is incorporated in filtrate, at 70 ℃, dries to obtain finished product 5.63g.
Case study on implementation 4:
(1) fresh leaflet camphor grass 1000g, cuts off 4cm, and 7 times of 90% ethanol extracts, and adds mortar to grind to form homogenate, standing, filters to get filtrate, and uses alcohol flushing mortar;
(2) by filter residue process squeezing machine, must remain fresh juice, filter, will after all filtrate merging, add gasoline, fully stir, be placed in respectively separating funnel, more than sufficient standing 2.5h, liquid is divided into three layers.
(3) upper strata is oil reservoir, is blackish green, and lower floor is that yellow liquid claims water layer, and middle level is suspended on water layer, under upper strata, claims the greyish white beds of precipitation.Water layer, the greyish white beds of precipitation, oil reservoir are filtered respectively, and filter residue merges, and with distilled water wash, drains, and in 100 ℃ of oven dry, obtains vegetable protein 82.1g; By oil reservoir decompression recycling ethanol and gasoline, obtain chlorophyll paste 5.91g;
(4) with 10% the sodium hydroxide solution that the chlorophyll alcohol liquid of 4 volumes adds 1 volume, carry out saponification, temperature 60 C, saponification 1h, stratification, lower floor is chlorophillins saponification liquor;
(5), by chlorophyll saponification liquor petroleum ether extraction oil-soluble impurities, extraction secondary adds equivalent sherwood oil in alcohol saponification liquor at every turn, stirs stratification, emits petroleum ether layer, obtains extraction liquid;
(6) extraction liquid regulates pH value to subacidity, adds 20% copper sulfate solution, then is adjusted at 3,60 ℃ of pH values and keeps 60min, fully puts copper.
(7) filter, with 90% washing with alcohol 1-2 time, washings and filtrate merging, the water that doubles dilutes, and chlorophyll cupric acid can be separated out, and dries and to obtain the sour 5.77g of the green copper of solid leaf, and the Diluted Alcohol solution washing with 40% several times, is filtered dry and gets final product purifying
(8) add 5% sodium hydroxide solution, dissolved copper acid, is added to PH always and is till 11, and salification process is first early thick, with sodium hydroxide, adds, gradually thinning,, until during PH 11, retrogradation again again.After salify, filter, filter residue rinses 1-2 time with distilled water, and washings is incorporated in filtrate, at 80 ℃, dries to obtain finished product 5.59g.
Claims (1)
1. utilize two-step approach extracting chlorophyll and a vegetable protein, chlorophyll paste is through alkali lye saponification, and copper is replaced as the novel method of sodium copper chlorophyllin, and its step is as follows:
(1) chlorophyll is just carried: fresh leaflet camphor grass cuts off 3-5cm, and 6-8 times of 80-90% ethanol extracts, and adds mortar to grind to form homogenate, standing, filters to get filtrate, and uses alcohol flushing mortar, and filtrate merges;
(2) filter residue second extraction: filter residue, through squeezing machine, must be remained to fresh juice, filter, after all filtrate is merged, add gasoline, fully stir, be placed in separating funnel, sufficient standing 2-3h, liquid is divided into three layers;
(3) obtain primary extract: upper strata is oil reservoir, be blackish green, lower floor is that yellow liquid claims water layer, middle level is suspended on water layer, under upper strata, claims the greyish white beds of precipitation, water layer, the greyish white beds of precipitation, oil reservoir are filtered respectively, filter residue merges, and with distilled water wash, drains, and in 90-100 ℃ of oven dry, obtains vegetable protein; By oil reservoir decompression recycling ethanol and gasoline, obtain chlorophyll paste;
(4) saponification: the sodium hydroxide solution of processing the 5%-10% of 1 volume with the chlorophyll alcohol liquid of 4 volumes carries out saponification, temperature 50-60 ℃, saponification 1h, stratification, lower floor is chlorophillins saponification liquor;
(5) extraction: by chlorophyll saponification liquor petroleum ether extraction oil-soluble impurities, extract three times, add equivalent sherwood oil at every turn in alcohol saponification liquor, stir stratification, emit petroleum ether layer, obtain extraction liquid;
(6) put copper: extraction liquid regulates pH value to subacidity, add 20% copper sulfate solution, then be adjusted to pH value 2-3, keep 30-60min at 60 ℃, fully put copper;
(7) purifying: filter, by 90% washing with alcohol 1-2 time, washings merges with filtrate, and the water that doubles dilutes, and chlorophyll cupric acid can be separated out, dries that to obtain the green copper of solid leaf sour, uses the Diluted Alcohol solution washing of 40-50% several times, is filtered dry and gets final product purifying;
(8) salify: add 5% sodium hydroxide solution, dissolved copper acid, is added to pH always and is till 11, and it is thick that salification process is first, with sodium hydroxide, add, gradually thinning, until pH is 11 o'clock, retrogradation again again, after salify, filter, filter residue distilled water flushing 1-2 time, washings is incorporated in filtrate, 60-80 ℃ of vacuum hydro-extraction, obtains finished product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210144911.2A CN102731513B (en) | 2011-12-25 | 2012-05-06 | Method for extracting sodium copper chlorophyllin from wild deyeuxia angustifolia forage grass |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110462656.1 | 2011-12-25 | ||
| CN201110462656 | 2011-12-25 | ||
| CN201210144911.2A CN102731513B (en) | 2011-12-25 | 2012-05-06 | Method for extracting sodium copper chlorophyllin from wild deyeuxia angustifolia forage grass |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102731513A CN102731513A (en) | 2012-10-17 |
| CN102731513B true CN102731513B (en) | 2014-11-19 |
Family
ID=46987890
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210144911.2A Active CN102731513B (en) | 2011-12-25 | 2012-05-06 | Method for extracting sodium copper chlorophyllin from wild deyeuxia angustifolia forage grass |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102731513B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103509028B (en) * | 2013-10-11 | 2015-08-12 | 中国科学院烟台海岸带研究所 | The preparation method of a kind of chlorophyll cupric acid and copper sodium |
| CN105086502A (en) * | 2015-09-08 | 2015-11-25 | 湖州宏鑫绸厂 | Novel nanometer titania/chlorophyll derivative dye and preparation method thereof |
| CN105230963A (en) * | 2015-11-02 | 2016-01-13 | 湖北民族学院 | Method for comprehensively extracting leaf protein and chlorophyll from ginger leaves |
| CN109705127B (en) * | 2018-12-24 | 2020-04-14 | 南京斯拜科生化实业有限公司 | Anti-emulsification preparation method of plant-derived sodium copper chlorophyllin |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101591339A (en) * | 2008-05-28 | 2009-12-02 | 北京健健康康生物技术有限公司 | A kind of is the method for feedstock production chlorophyll copper sodium with the watermelon outer peel |
| CN102199153A (en) * | 2011-03-29 | 2011-09-28 | 晨光生物科技集团天津有限公司 | Method for preparing sodium copper chlorophyllin by using silkworm excrement as raw material |
-
2012
- 2012-05-06 CN CN201210144911.2A patent/CN102731513B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101591339A (en) * | 2008-05-28 | 2009-12-02 | 北京健健康康生物技术有限公司 | A kind of is the method for feedstock production chlorophyll copper sodium with the watermelon outer peel |
| CN102199153A (en) * | 2011-03-29 | 2011-09-28 | 晨光生物科技集团天津有限公司 | Method for preparing sodium copper chlorophyllin by using silkworm excrement as raw material |
Non-Patent Citations (4)
| Title |
|---|
| 丁利君等.芥蓝叶制取叶绿素铜钠盐的工艺参数及其性质研究.《现代食品科技》.2005,第21卷(第1期),第36-38页. * |
| 大叶黄杨叶制备叶绿素铜钠盐的工艺研究;王晓等;《山东科学》;20011215;第14卷(第4期);正文部分第2.2、2.3节 * |
| 王晓等.大叶黄杨叶制备叶绿素铜钠盐的工艺研究.《山东科学》.2001,第14卷(第4期),第14-17页. * |
| 芥蓝叶制取叶绿素铜钠盐的工艺参数及其性质研究;丁利君等;《现代食品科技》;20050330;第21卷(第1期);正文部分第1.2节 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102731513A (en) | 2012-10-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106497720B (en) | The deep working method of " four slags " in Kiwi berry complete utilization | |
| CN103463123B (en) | Method for extracting effective constituents from maca | |
| CN102731513B (en) | Method for extracting sodium copper chlorophyllin from wild deyeuxia angustifolia forage grass | |
| CN101779705B (en) | Production method of grape seed oil | |
| US20080181999A1 (en) | Water Leaching Method to Extract Oil Plant Products | |
| CN102796610A (en) | Production technology for extracting eucommia oil and eucommia protein from eucommia kernels | |
| CN104725341A (en) | Efficient extraction method for blueberry anthocyanin | |
| CN102558264A (en) | Method for extracting cordycepin and cordyceps polysaccharide from cordyceps militaris | |
| CN101449801A (en) | Lycopene extraction method from tomato peel | |
| CN103525542B (en) | Method for extracting shiny-leaved yellowhorn grease containing nervonic acid from shiny-leaved yellowhorn | |
| CN101974096B (en) | Method for efficiently extracting polysaccharides from sporophyll of Undaria pinnatifida Suringar | |
| CN103937604A (en) | Method for extracting oil in microalgae | |
| CN103301172A (en) | Method for coproduction of pistacia chinensis bunge essential oil and polyphenol | |
| CN109054992A (en) | A method of extracting Seabuckthorm Seed Oil | |
| CN103820204B (en) | The preparation method of tuna liver oil | |
| CN102180921A (en) | Method for extracting high-purity rutin from boxthorn leaves | |
| CN101869265A (en) | Method for using enzyme method for assisting aqueous extraction of insoluble dietary fiber from pollen pini | |
| CN1837234B (en) | Process for preparing low phytic acid rapeseed protein concentrate by using water and ethanol | |
| CN1943372A (en) | Linolenic acid health edible oil and prickly ash seed dregs and its preparing method | |
| CN105566507A (en) | Preparation method of tea polysaccharides | |
| CN105497101A (en) | Method for extracting kinds of combined extract from blueberry stems and leaves | |
| CN105037747A (en) | Method for extracting fulvic acid from fermented products of kitchen waste | |
| CN105295434B (en) | Method for combined extraction of haematochrome and pectin from pitaya peel | |
| CN105886103A (en) | Linolenic acid health care edible oil, Chinese prickly ash seed meal and preparation method thereof | |
| CN103584233A (en) | High-sugar-degree sweet potato juice and method for processing high-sugar-degree sweet potato juice |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C56 | Change in the name or address of the patentee |
Owner name: GREATER KHINGAN MOUNTAIN LINGONBERRY BIOTECHNOLOGY Free format text: FORMER NAME: GREAT XINGAN MOUNTAINS LINGEBEI ORGANIC FOOD CO., LTD. |
|
| CP03 | Change of name, title or address |
Address after: Small town Songling District 165000 Yongxing street be immensely proud of Heilongjiang area of Greater Khingan Range Province Patentee after: Daxing'an Mountainrange Lin Gebei psychrophile Science and Technology Co., Ltd. Address before: 165000 Heilongjiang province Jiagedaqi Xinyuan District Road Beilin Gebe AGA group Patentee before: Great Xingan Mountains Lingebei Organic Food Co., Ltd. |