CN102731290A - Method for extracting EPA and DHA from algae - Google Patents
Method for extracting EPA and DHA from algae Download PDFInfo
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- CN102731290A CN102731290A CN2011101061757A CN201110106175A CN102731290A CN 102731290 A CN102731290 A CN 102731290A CN 2011101061757 A CN2011101061757 A CN 2011101061757A CN 201110106175 A CN201110106175 A CN 201110106175A CN 102731290 A CN102731290 A CN 102731290A
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Abstract
The invention provides a method for extracting EPA and DHA from algae, which relates to the technical field of biochemical pharmacy. The method is realized by implementing the following technical scheme: the method for extracting EPA and DHA from algae is characterized by comprising the successive steps of crushing alga raw materials, adding hydrochloric acid for hydrolysis, carrying out grinding and homogenizing by using a colloid mill and then successively carrying out ultrafiltration, nanofiltration I, nanofiltration II and ethanol extraction. Compared with the prior art, the invention has the following striking substantive advantages and remarkable progress: usage of traditional extraction methods and harmful organic solvents is reduced, original biological activity is retained during the process of extraction, the extraction process is simple and environment-friendly, and the method costs little and is applicable to large scale industrial production.
Description
Technical field
The present invention relates to biological chemistry pharmaceutical technology field, relate in particular to a kind of method and technology field from extracting algae EPA and DHA.
Background technology
DHA; The formal name used at school docosahexenoic acid; Be the requisite highly unsaturated fatty acids of brain nutrition, it the more important thing is that except stoping the deposition of SUV on vessel wall, prevent or alleviating the generation of atherosclerosis and coronary heart disease DHA has important role to brain cell.It has accounted for 10% of human brain fat, and is very favourable to growing of cranial nerve conduction and cynapse.DHA is as a kind of indispensable fatty acid, and effects such as its hypermnesis and thinking ability, raising intelligence are more remarkable.Crowd's epidemiological study finds that the people's that DHA content is high in the body psychological endurance power is strong, MDI is also high.DHA influences fetal brain development: in the pregnancy period, DHA can optimize the constituent of fetal brain pyramidal cell's phosphatide.Especially fetus is full after 5 months, and as artificially the sense of hearing, vision, the sense of touch of fetus being stimulated, it is prominent to cause that the sensorial neurone of fetal brain cortex increases more number, and this just needs parent to supply with the more DHA of fetus simultaneously.DHA promotes the maturation of retina light sensation cell, and DHA not only has material impact to fetal brain development, and the maturation of retina light sensation cell is played an important role.The pregnant woman was at pregnant last three months, and the a-linolenic acid in female blood capable of using synthesizes DHA, is transported to fetal brain and retina then, and neurocyte ripening degree there is improved.So mother should eat of the food (like ocean fish) that contains DHA in the pregnancy period more, in the end 3 pregnant month, also should eat more and contain the many food (like hard fruit) of a-linolenic acid, the person of having ready conditions can directly replenish from a-linolenic acid or DHA nutritious prod.EPA formal name used at school timnodonic acid is the staple of fish oil.EPA belongs to the serial pufas of Ω-3, is that human body self can not synthesize but indispensable important nutrient, therefore is called essential fatty acid.Though linolenic acid can be converted into EPA in human body, this is reflected at the very slow and inversion quantity of speed in the human body seldom, can not satisfy the needs of human body to EPA far away, therefore must from food, directly replenish.EPA has the content that helps reducing cholesterol and triglyceride level, promotes saturated fatty acid metabolic in the body.Thereby play blood viscosity lowering, promote blood circulation, improve and organize oxygen supply and Ginseng Extract.Prevent the deposition of fat, cardiovascular disordeies such as the formation and development that prevention of arterial is atherosis, prevention of brain thrombus, Intracerebral hemorrhage, hypertension at vessel wall.
Summary of the invention
Technical problem to be solved by this invention is the deficiency that overcomes prior art; And a kind of usage quantity new, that can reduce traditional extraction process and harmful organic solvent is provided; In leaching process, keep original biological activity, make that leaching process is simple, environmental protection, cost is low and can carry out the method from extracting algae EPA and DHA of large-scale industrial production.
For solving the problems of the technologies described above, the present invention is achieved through implementing following technical scheme; A kind of method from extracting algae EPA and DHA, its special character are that described method comprises the steps: the marine alga raw material pulverizing successively, add hydrochloric acid hydrolysis, after colloidal mill grinds homogenate, advance ultrafiltration, nanofiltration I, nanofiltration II successively, extraction using alcohol.
For further solving the problems of the technologies described above, the preferred version of technique scheme is:
Above-mentioned described marine alga is sea-tangle, Sargassum fusiforme, bladder wrack, black wrack any one or its mixing.
The gap of above-mentioned described colloidal mill is adjusted at 0.1-3mm.
Above-mentioned described ultrafiltration be with above-mentioned homogenate can molecular weight cut-off 10000 dalton in the rolling ultra-filtration equipment of above material, under the pressure of 0.01-0.05MPA, adopt cross flow filter to remove waste residue and get just and filtrate.
Above-mentioned described said nanofiltration I is can molecular weight cut-off be in the nanofiltration device of the above macromolecular substance of 400 dalton with above-mentioned filtrating just, under the pressure of 0.01-0.15MPA, carries out a nanofiltration and removes macromolecular substance and must filtrate.
Above-mentioned described nanofiltration II be with above-mentioned filtrating can elimination molecular weight be in the nanofiltration device of the following material of 300 dalton, under the pressure of 0.01-0.15MPA, carry out the secondary nanofiltration and remove impurity, its trapped substance is EPA and DHA first product.
Above-mentioned described ethanol sedimentation is with above-mentioned EPA and the DHA first product places in the whisking appliance and by weight 1: 1.5-2.5 adds 70-98% ethanol, stirs and promptly forms gel in 0.5-1 hour.
The present invention compared with prior art has following outstanding substantive distinguishing features and obvious improvement: in the first method of the present invention except that in the mixing homogenate that extracts EPA and DHA, being added with hydrochloric acid and the ethanol afterwards; No longer add any deleterious organic solvent; All utilize the mechanical-physical method and extract the preparation process; Reach the usage quantity that reduces traditional extraction process and harmful organic solvent fully, make that the preparation process reaches simply, the standard-required of environmental protection; Improved the security of preparation process, eliminated the potential safety hazard of prior art and the pollution of environment.It two is that method of the present invention is all extracted through specific equipment; Or else whole described method is added under the prerequisite of any harmful organic solvent and is separated, purifies; Thereby make EPA of the present invention and DHA keep original characteristic; Not only reach improve EPA and DHA be used for food, medicine, range of application, and improved security, the safety of use; It three is the process for extracting of EPA of the present invention and DHA had both found renewable resource for marine alga the methods of utilizing; Take to use mechanical means to exercise again and extract the process of EPA and DHA, thus make the process for extracting of EPA of the present invention and DHA compared with prior art have implementation process simple, reliably, environmental protection, cost be low, the substantive distinguishing features and the obvious improvement of giving prominence to that can carry out large-scale industrial production.
Embodiment
Embodiment 1:
A kind of method of the embodiment of the invention 1 from extracting algae EPA and DHA; Comprise the steps: that successively particle diameter is washed and be crushed to marine alga is 2-3mm; Add the aqueous hydrochloric acid dissolving of PH2-3 then, after colloidal mill grinds homogenate, advanced ultrafiltration, nanofiltration I, nanofiltration II successively; Ethanol sedimentation, spraying drying and process pure article.Practical implementation is following:
1, pulverizes.Material 10kg after handling is placed in the kibbler, material is crushed to particle diameter 2-3mm, add the aqueous hydrochloric acid dissolving of PH2-3;
2, colloidal mill grinds.With above-mentioned mixed solution, be the colloidal mill grinding of 3mm through the gap;
3, ultrafiltration be with liquid after the above-mentioned grinding can molecular weight cut-off 10000 dalton in the rolling ultra-filtration equipment of above material, under the pressure of 0.01-0.02MPA, adopt cross flow filter to remove waste residue and get just and filtrate;
4, nanofiltration I is can molecular weight cut-off be in the nanofiltration device of the above macromolecular substance of 400 dalton with above-mentioned filtrating just, under the pressure of 0.01-0.02MPA, carries out a nanofiltration and removes macromolecular substance and must filtrate;
5, nanofiltration II be with above-mentioned filtrating can elimination molecular weight be in the nanofiltration device of the following material of 300 dalton, under the pressure of 0.01-0.02MPA, carry out the secondary nanofiltration and remove impurity, its trapped substance is EPA and DHA first product;
6, ethanol sedimentation: be above-mentioned EPA and DHA first product to be placed in the whisking appliance and by weight 1: 1.5 add 98% ethanol, stir and promptly formed gel in 0.5 hour;
7, press filtration is that gel with EPA and DHA places the gelling thing that under the pressure of 0.2Mpa, makes EPA and DHA in the plate-and-frame filter press;
8, the solution that above-mentioned press filtration is gone out distills, to reach recycling ethanol;
9, above-mentioned gelling thing is carried out spraying drying, be prepared into EPA and DHA dry product; Promptly got EPA and DHA powder finished product through pulverizing 80 mesh sieves.
It more than is a kind of method of the embodiment of the invention 1 from extracting algae EPA and DHA.
Embodiment 2:
A kind of method of the embodiment of the invention 2 from extracting algae EPA and DHA; Comprise the steps: that successively it is 2-3mm that marine alga and tankage are cleaned and be crushed to particle diameter; The aqueous hydrochloric acid dissolving that adds PH2-3 then; With 1: 10 mixed of marine alga siccative water after after colloidal mill grinds homogenate,, press filtration, ultrafiltration, nanofiltration I, nanofiltration II, stir into glue, press filtration, distillation reuse, spraying drying and pulverizing and process pure article.Practical implementation is following:
1, pulverizes.Material 10kg after handling is placed in the kibbler, material is crushed to particle diameter 2-3mm, add the aqueous hydrochloric acid dissolving of PH2-3;
2, colloidal mill grinds.With above-mentioned mixed solution, be the colloidal mill grinding of 3mm through the gap;
3, ultrafiltration be with liquid after the above-mentioned grinding can molecular weight cut-off 10000 dalton in the rolling ultra-filtration equipment of above material, under the pressure of 0.02-0.03MPA, adopt cross flow filter to remove waste residue and get just and filtrate;
4, nanofiltration I is can molecular weight cut-off be in the nanofiltration device of the above macromolecular substance of 400 dalton with above-mentioned filtrating just, under the pressure of 0.03-0.04MPA, carries out a nanofiltration and removes macromolecular substance and must filtrate;
5, nanofiltration II be with above-mentioned filtrating can elimination molecular weight be in the nanofiltration device of the following material of 300 dalton, under the pressure of 0.030.04MPA, carry out the secondary nanofiltration and remove impurity, its trapped substance is EPA and DHA first product;
6, ethanol sedimentation: be above-mentioned EPA and DHA first product to be placed in the whisking appliance and by weight 1: 1.5 add 98% ethanol, stir and promptly formed gel in 0.5 hour;
7, press filtration is that gel with EPA and DHA places the gelling thing that under the pressure of 0.2Mpa, makes EPA and DHA in the plate-and-frame filter press;
8, the solution that above-mentioned press filtration is gone out distills, to reach recycling ethanol;
9, above-mentioned gelling thing is carried out spraying drying, be prepared into EPA and DHA dry product; Promptly got EPA and DHA powder finished product through pulverizing 80 mesh sieves.
It more than is a kind of method of the embodiment of the invention 2 from extracting algae EPA and DHA.
Embodiment 3:
A kind of method of the embodiment of the invention 3 from extracting algae EPA and DHA; Comprise the steps: that successively it is 2-3mm that marine alga and tankage are cleaned and be crushed to particle diameter; The aqueous hydrochloric acid dissolving that adds PH2-3 then; 1: 10 mixed of marine alga siccative water after after colloidal mill grinds homogenate, press filtration, ultrafiltration, nanofiltration I, nanofiltration II, is stirred into glue, press filtration, distillation reuse, spraying drying and pulverizing and processes pure article.Practical implementation is following:
1, pulverizes.Material 10kg after handling is placed in the kibbler, material is crushed to particle diameter 2-3mm, add the aqueous hydrochloric acid dissolving of PH2-3;
2, colloidal mill grinds.With above-mentioned mixed solution, be the colloidal mill grinding of 3mm through the gap;
3, ultrafiltration be with liquid after the above-mentioned grinding can molecular weight cut-off 10000 dalton in the rolling ultra-filtration equipment of above material, under the pressure of 0.03-0.04MPA, adopt cross flow filter to remove waste residue and get just and filtrate;
4, nanofiltration I is can molecular weight cut-off be in the nanofiltration device of the above macromolecular substance of 400 dalton with above-mentioned filtrating just, under the pressure of 0.06-0.08MPA, carries out a nanofiltration and removes macromolecular substance and must filtrate;
5, nanofiltration II be with above-mentioned filtrating can elimination molecular weight be in the nanofiltration device of the following material of 300 dalton, under the pressure of 0.06-0.08MPA, carry out the secondary nanofiltration and remove impurity, its trapped substance is EPA and DHA first product;
6, ethanol sedimentation: be above-mentioned EPA and DHA first product to be placed in the whisking appliance and by weight 1: 1.5 add 98% ethanol, stir and promptly formed gel in 0.5 hour;
7, press filtration is that gel with EPA and DHA places the gelling thing that under the pressure of 0.2Mpa, makes EPA and DHA in the plate-and-frame filter press;
8, the solution that above-mentioned press filtration is gone out distills, to reach recycling ethanol;
9, above-mentioned gelling thing is carried out spraying drying, be prepared into EPA and DHA dry product; Promptly got EPA and DHA powder finished product through pulverizing 80 mesh sieves.
More than be a kind of method of the embodiment of the invention 3 from extracting algae EPA and DHA.
Embodiment 4:
A kind of method of the embodiment of the invention 4 from extracting algae EPA and DHA; Comprise the steps: that successively it is 2-3mm that marine alga and tankage are cleaned and be crushed to particle diameter; The aqueous hydrochloric acid dissolving that adds PH2-3 then; With 1: 10 mixed of marine alga siccative water after after colloidal mill grinds homogenate,, ultrafiltration, nanofiltration I, nanofiltration II, stir into glue, press filtration, distillation reuse, spraying drying and pulverizing and process pure article.Practical implementation is following:
1, pulverizes.Material 10kg after handling is placed in the kibbler, material is crushed to particle diameter 2-3mm, add the aqueous hydrochloric acid dissolving of PH2-3;
2, colloidal mill grinds.With above-mentioned mixed solution, be the colloidal mill grinding of 3mm through the gap;
3, ultrafiltration be with liquid after the above-mentioned grinding can molecular weight cut-off 10000 dalton in the rolling ultra-filtration equipment of above material, under the pressure of 0.1-0.12MPA, carry out a nanofiltration and remove macromolecular substance and must filtrate;
4, nanofiltration I is can molecular weight cut-off be in the nanofiltration device of the above macromolecular substance of 400 dalton with above-mentioned filtrating just, under the pressure of 0.1-0.12MPA, carries out a nanofiltration and removes macromolecular substance and must filtrate;
5, nanofiltration II be with above-mentioned filtrating can elimination molecular weight be in the nanofiltration device of the following material of 300 dalton, under the pressure of 0.1-0.12MPA, carry out the secondary nanofiltration and remove impurity, its trapped substance is EPA and DHA first product;
6, ethanol sedimentation: be above-mentioned EPA and DHA first product to be placed in the whisking appliance and by weight 1: 1.5 add 98% ethanol, stir and promptly formed gel in 0.5 hour;
7, press filtration is that gel with EPA and DHA places the gelling thing that under the pressure of 0.2Mpa, makes EPA and DHA in the plate-and-frame filter press;
8, the solution that above-mentioned press filtration is gone out distills, to reach recycling ethanol;
9, above-mentioned gelling thing is carried out spraying drying, be prepared into EPA and DHA dry product; Promptly got EPA and DHA powder finished product through pulverizing 80 mesh sieves.
More than be a kind of method of the embodiment of the invention 4 from extracting algae EPA and DHA.
Embodiment 5:
A kind of method of the embodiment of the invention 5 from extracting algae EPA and DHA; It is characterized in that described method comprises the steps: that successively it is 2-3mm that marine alga and tankage are cleaned and be crushed to particle diameter; The aqueous hydrochloric acid dissolving that adds PH2-3 then; 1: 10 mixed of marine alga siccative water after after colloidal mill grinds homogenate, ultrafiltration, nanofiltration I, nanofiltration II, is stirred into glue, press filtration, distillation reuse, spraying drying and pulverizing and processes pure article.Practical implementation is following:
1, pulverizes.Material 10kg after handling is placed in the kibbler, material is crushed to particle diameter 2-3mm, add the aqueous hydrochloric acid dissolving of PH2-3;
2, colloidal mill grinds.With above-mentioned mixed solution, be the colloidal mill grinding of 3mm through the gap;
3, ultrafiltration be with liquid after the above-mentioned grinding can molecular weight cut-off 10000 dalton in the rolling ultra-filtration equipment of above material, under the pressure of 0.03-0.04MPA, adopt cross flow filter to remove waste residue and get just and filtrate;
4, nanofiltration I is can molecular weight cut-off be in the nanofiltration device of the above macromolecular substance of 400 dalton with above-mentioned filtrating just, under the pressure of 0.14-0.15MPA, carries out a nanofiltration and removes macromolecular substance and must filtrate;
5, nanofiltration II be with above-mentioned filtrating can elimination molecular weight be in the nanofiltration device of the following material of 300 dalton, under the pressure of 0.14-0.15MPA, carry out the secondary nanofiltration and remove impurity, its trapped substance is EPA and DHA first product;
6, ethanol sedimentation: be above-mentioned EPA and DHA first product to be placed in the whisking appliance and by weight 1: 1.5 add 98% ethanol, stir and promptly formed gel in 0.5 hour;
7, press filtration is that gel with EPA and DHA places the gelling thing that under the pressure of 0.2Mpa, makes EPA and DHA in the plate-and-frame filter press;
8, the solution that above-mentioned press filtration is gone out distills, to reach recycling ethanol;
9, above-mentioned gelling thing is carried out spraying drying, be prepared into EPA and DHA dry product; Promptly got EPA and DHA powder finished product through pulverizing 80 mesh sieves.
It more than is a kind of method of the embodiment of the invention 5 from extracting algae EPA and DHA.
Claims (7)
1. the method from extracting algae EPA and DHA is characterized in that described method comprises the steps: the marine alga raw material pulverizing successively, adds hydrochloric acid hydrolysis, after colloidal mill grinds homogenate, advances ultrafiltration, nanofiltration I, nanofiltration II successively, extraction using alcohol.
2. the method from extracting algae EPA and DHA according to claim 1 is characterized in that described marine alga is sea-tangle, Sargassum fusiforme, bladder wrack, black wrack any one or its mixing.
3. the method from extracting algae EPA and DHA according to claim 1 is characterized in that the gap of described colloidal mill is adjusted at 0.1-3mm.
4. the method from extracting algae EPA and DHA according to claim 1; It is characterized in that described ultrafiltration be with above-mentioned homogenate can molecular weight cut-off 10000 dalton in the rolling ultra-filtration equipment of above material, under the pressure of 0.01-0.05MPA, filter and remove waste residue and get just and filtrate.
5. the method from extracting algae EPA and DHA according to claim 1; It is characterized in that said nanofiltration I is can molecular weight cut-off be in the nanofiltration device of the above macromolecular substance of 400 dalton with above-mentioned filtrating just, under the pressure of 0.01-0.15MPA, carry out a nanofiltration and remove macromolecular substance and must filtrate.
6. the method from extracting algae EPA and DHA according to claim 1; It is characterized in that described nanofiltration II be with above-mentioned filtrating can elimination molecular weight be in the nanofiltration device of the following material of 300 dalton; Under the pressure of 0.01-0.15MPA, carry out the secondary nanofiltration and remove impurity, its trapped substance is EPA and DHA first product.
7. the method from extracting algae EPA and DHA according to claim 1, it is characterized in that described ethanol sedimentation is with above-mentioned EPA and the DHA first product places in the whisking appliance and by weight 1: 1.5-2.5 adds 70-98% ethanol, stirs 0.5-1 hour.
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| CN2011101061757A CN102731290A (en) | 2011-04-15 | 2011-04-15 | Method for extracting EPA and DHA from algae |
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| CN2011101061757A CN102731290A (en) | 2011-04-15 | 2011-04-15 | Method for extracting EPA and DHA from algae |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113480425A (en) * | 2021-06-21 | 2021-10-08 | 日照职业技术学院 | Process for extracting DHA and EPA from microalgae |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113480425A (en) * | 2021-06-21 | 2021-10-08 | 日照职业技术学院 | Process for extracting DHA and EPA from microalgae |
| CN113480425B (en) * | 2021-06-21 | 2023-07-18 | 日照职业技术学院 | Process for extracting DHA and EPA from microalgae |
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Application publication date: 20121017 |