CN102727900A - Chicken coccidiosis vaccine suspending agent and fluid suspension therewith - Google Patents
Chicken coccidiosis vaccine suspending agent and fluid suspension therewith Download PDFInfo
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- CN102727900A CN102727900A CN2012102306597A CN201210230659A CN102727900A CN 102727900 A CN102727900 A CN 102727900A CN 2012102306597 A CN2012102306597 A CN 2012102306597A CN 201210230659 A CN201210230659 A CN 201210230659A CN 102727900 A CN102727900 A CN 102727900A
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Abstract
The invention discloses a chicken coccidiosis vaccine suspension agent and suspension fluid therewith. The chicken coccidiosis vaccine suspension agent comprises xanthan gum and aqueous ethanol, wherein the volume concentration of the aqueous ethanol is greater than 38% and smaller then or equal to 100%, and a weight-to-volume ratio of xanthan gum to aqueous ethanol is from 3% to 60%. Xanthan gum is preferably selected fromfood-grade and pharmaceutical grade xanthan gum, and ethanol is preferably selected from food-grade and pharmaceutical grade ethanol. The invention further discloses suspension fluid prepared by using the suspending agent. The invention is advantaged in that: first, the suspension agent can dissolve in water rapidly; second, prepared chicken coccidiosis live vaccine oocysts can be kept in water by the prepared suspension fluid for 8 hour without sedimentation such that quantity uniformity of vaccine oocysts drunk by chickens can be improved; third, non-water-stopping chicken flock immunization can be carried out, and water-stopping stress response is reduced; fourth, water-stopping chicken flock immunization can further be carried out, and immunization time is shortened; and fifth, a side effect of the vaccine is reduced.
Description
Technical field
The present invention relates to a kind of chicken coccidiosis vaccine suspending agent and suspension, the chicken coccidiosis that is specifically related to a kind of immunity that is used to the to drink water suspending agent and the suspension of egg capsule vaccine of living, this suspending agent can dissolve fast; The sinking that can slow down the vaccine egg capsule again reaches 8 hours, and chicken crowd's do not cut off the water immunity or the immunity of cutting off the water can both be achieved, and can both reduce the side reaction of vaccine under two kinds of situation.
Background technology
Chicken coccidiosis is that a kind of protozoon parasite is sick, by tender eimeria tenella, heap shape eimeria tenella, huge eimeria tenella, poison eimeria tenella, gentle eimeria tenella, Bu Shi eimeria tenella, precocious eimeria tenella one or more cause.They are parasitic at each position of the intestinal of chicken, cause the inflammation of intestinal, the death of hemorrhage and chicken, cause enormous economic loss to poultry husbandry.
For a long time, the control of this disease is main with medicine.Because drug residue influences food safety and human health, adds that coccidiosis is easy to develop immunity to drugs to medicine, the control of this disease more and more relies on immune means.
The chicken coccidiosis vaccine that uses at present mainly is live egg capsule vaccine, the for example LIVACOX of the IMMUCOX of the PARACOX of Schering Plough and COCCIVAC, Canadian Wei Taike, Cimmeria.Wherein COCCIVAC has nearly 60 years history (R.B.Williams, Avian disease46:775-802,2002, Historical article-Fifty years of Anticoccidial vaccines for poultry (1952--2002)).
The chicken coccidiosis vaccine can be inoculated to chicken in many ways, like spraying, spray material, spice, drip, oral gel, drinking-water etc.All said methods all can't thoroughly solve the problem of immune uniformity and side reaction; Be how much egg capsule quantity that every chicken is taken in differs; The immunity that produces also height differs, and when chicken was had offspring's egg capsule of the immune coccidial vaccine of institute from bedding and padding, those immunity were lower and take in the side reaction that vaccine immunity just appears in egg capsule more chicken easily; Abnormal conditions such as feces is unusual, hemafecia, death occur, influence the use of raise chickens production and coccidial vaccine.
The drinking-water immunity has advantages such as mixing is even, the preservation of vaccine vigor is better.The drinking-water immunity can be divided into the cut off the water immunity and the immune dual mode of not cutting off the water.The immunity of cutting off the water was meant before immunity breaks off the water source to chicken, and chicken is drunk in a period of time less than water, and when beginning to drink water immunity, all chickens begin to drink water simultaneously, and the immunity of cutting off the water can make the whole immune operation time shorten; But to chicken can produce to a certain degree stress.The immunity of not cutting off the water is not meant breaks off the water source to chicken, can reduce cut off the water stress, but the whole immunologic process persistent period is longer, the time lengthening can influence the vigor of vaccine.During modern times raise chickens and produce, raise with management personnel's efficient very highly, very busy, above dual mode respectively has advantage, and the production of all being raised chickens is adopted.
During the drinking-water immunity, mixed vaccine egg capsule precipitates easily, and the vaccine egg capsule of chicken acquisition when the drinking-water immunity will occur than big-difference like this, and uniformity is relatively poor.
Xanthan gum is claimed HUANGJIAO, HANSHENGJIAO again, and yellow unit cell polysaccharide is a kind of monospore polysaccharide that is produced by false xanthomonas fermentation; By the cabbage black rot xanthomonas campestris is primary raw material with the carbohydrate, through the aerobic fermentation biotechnology, cuts off 1; The 6-glycosidic bond; After opening side chain, by 1,4-is bonded to the outer heteropolysaccharide of a kind of acid born of the same parents that straight chain is formed.Nineteen fifty-two is separated the cabbage black rot Xanthomonas campestris that obtains by the northern institute in United States Department of Agriculture (USDA) Illinois skin Lille difficult to understand, and makes the Caulis et Folium Brassicae capitatae extract be converted into the outer heteropolysaccharide of water miscible acid born of the same parents and obtain.Xanthan gum is to collect thickening, suspension, emulsifying at present in the world, be stable at one. the biogum of superior performance.But because it has extremely strong hydrophilic, if directly add in the entry, outer imbibition becomes micelle, and micelle wraps the dry powder of nexine, can stop moisture to get into nexine, thereby slow down its dissolution velocity greatly.The mixture of being made up of xanthan gum, ethanol water can be dissolved in the water fast, does not form micelle.
In the time will referring to this new field of coccidial vaccine suspending agent by the mixture that xanthan gum, ethanol water are formed, the problem below at first must solving.1, used suspending agent does not have toxic action to chicken; 2, used suspending agent does not have lethal effect to coccidian oocyst; 3, used suspending agent does not have injury effect to infective zygoblast that has that coccidian oocyst discharges; 4, used suspending agent does not stop up waterline.
The suspending agent of report in the past, carboxymethyl cellulose for example, course of dissolution is very long.Wang Jiang wait clearly reported carboxymethyl cellulose as the effect of vaccine to chicken coccidiosis suspending agent (Wang Jiangqing etc. the research of the precocious attenuated strain drinking-water of tender eimeria tenella immunization method. Shandong animal and veterinary, 1999,04).But carboxymethyl cellulose dissolution velocity in water is slower, need in water, soak for a long time and could dissolve, and under the situation of repeatedly crumbing in manual work, needs ability dissolving in tens hours.So this cellulose ether is not a kind of ideal chicken coccidiosis vaccine suspending agent.
Change in modern times in the poultry husbandry, the chicken that each poultry raiser will feed is a lot, and labor intensity is very big, be busy with one's work, coccidiosis live the egg capsule vaccine suspending agent fast dissolving just can meet the raise chickens requirement of production of high efficiency modernization.How improving the suspending agent dissolution velocity, is a problem that urgency is to be solved.
Summary of the invention
The objective of the invention is provides a kind of chicken coccidiosis vaccine suspending agent and suspension for overcoming the deficiency of above-mentioned prior art.
For realizing above-mentioned purpose, the present invention adopts following technical proposals:
A kind of chicken coccidiosis vaccine suspending agent, it is made up of xanthan gum and ethanol water, and wherein the volumetric concentration of ethanol water is 38% ~ 100%, and xanthan gum and ethanol water are 3% ~ 60% in the ratio of g/ml.
The volumetric concentration of said ethanol water is 45% ~ 100%.
The volumetric concentration of preferred alcohol aqueous solution is 75%.
The ratio of xanthan gum and ethanol water is 3% ~ 24%.
Said is xanthan gum preferred food product level, pharmaceutical grade xanthan gum, and said ethanol is preferably food stage, pharmaceutical grade ethanol.
The present invention also provides a kind of suspending liquid by above-mentioned suspending agent preparation.
The invention has the beneficial effects as follows: one, suspending agent of the present invention can dissolve entry fast.Two, the suspension fluid power of preparation is kept egg capsule not sedimentation in 8 hours in water of chicken coccidiosis live vaccine, improves chicken and drinks the uniformity into vaccine egg capsule quantity.Three, the vaccinated flock of can not cutting off the water reduces that cut off the water stress.Four, the vaccinated flock of also can cutting off the water shortens the immunity time.Five, can reduce the side reaction of vaccine.
The specific embodiment
Through instantiation the present invention is further set forth below, should be noted that following explanation only is in order to explain the present invention, its content not to be limited.
One, the suspending agent of this invention to coccidiosis zygoblast nonhazardous effect, do not stop up waterline.
Through research repeatedly, the inventor finds that the suspending agent of this invention does not have toxic action and do not stop up waterline chicken, chicken coccidial oocyst, chicken coccidiosis zygoblast.
Waterline is meant at the pipeline of giving the chicken transporting water in producing of raising chickens, nipple-shape water fountain etc., and the impurity of the biomembrane fragment that they are easy to be come off, the medicine that drinking-water uses stops up, and is not easy cleaning.Through test, the phenomenon of waterline can not appear stopping up in suspending agent of the present invention.
Though chicken coccidial oocyst has thicker egg capsule wall, can stop the killing action of a lot of chemical substances; But after in the coccidian oocyst entering chicken body, the zygoblast in the egg capsule can discharge.According to professional general knowledge, lose the protective effect of egg capsule wall after, zygoblast can with chicken drink into suspending agent directly contact, zygoblast might receive chicken drink into the injury of suspending agent.
Embodiment 1. gets xanthan gum 3.6 grams, 25 milliliters of mixing of 95% ethanol water as suspending agent, pours in 6 kg of water this suspending agent and stirring, and this suspending agent dissolves very soon.Contain heap shape eimeria tenella, huge eimeria tenella, tender eimeria tenella, poison eimeria tenella 4 kinds of chicken coccidiosiss spore 2 milliliters of liquid of egg capsule (every milliliter contains 10,000 in spore egg capsule), shake up, be divided into 2 parts each 1 milliliter.20 of 4 age in days chickens are divided into 2 groups each 10.Get 9 milliliters of dissolved suspending agent solution of this invention and mix, give 1 group of chicken by each inoculation of amount of 1 milliliter of every chicken (every milliliter contains 1000 in spore egg capsule) with 1 part of chicken coccidiosis spore egg capsule liquid; The 2nd group of chicken do not add suspending agent solution, mixes with 1 part of chicken coccidiosis spore egg capsule liquid with 9 ml waters, inoculates to chicken by the amount of 1 milliliter of every chicken (every milliliter contains 1000 in spore egg capsule).That collects 2 groups of chickens contains egg capsule feces, calculates the egg capsule sum in the feces.Result such as table 1. (toxic action test).The egg capsule sum is basic identical in the feces of two groups of chickens.Suspending agent among proof the present invention does not have toxic action to the coccidiosis zygoblast.
Table 1. toxic action test
Two, the suspending agent of this invention can dissolve fast.
We find that the suspending agent of this invention can dissolve in the water fast.
Embodiment 2.
Get every part 3.6 gram of xanthan gum.Get volumetric concentration and be each 25 milliliters of 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50%, 55%, 65%, 75%, 85%, 95%, 100% ethanol waters.A ethanol water is mixed with a xanthan gum, pour in 6 kg of water after shaking up and stirring, this suspending agent dissolves very soon, and the result is shown in table 2..
The relation of table 2. concentration of alcohol and dissolution time
Experiment showed, rising, its enhanced dissolution rate along with concentration of alcohol; Particularly when the concentration of ethanol water was 45% ~ 100%, dissolution time was shorter, so the concentration of preferred alcohol aqueous solution is 45% ~ 100%.The concentration of ethanol water was got 75% o'clock, and the antiseptic effect of this solution is best, so preferred 75% ethanol water.
Embodiment 3.
Getting xanthan gum 3 gram, 6 grams, 9 grams, 12 grams, 15 grams, 18 grams, 21 grams, 24 grams, 27 grams, 30 grams, 33 grams, 36 grams, 39 grams, 42 grams, 45 grams, 48 grams, 51 grams, 54 grams, 57 grams, 60 grams mixes with the ethanol water of 100 milliliter 50% (volume ratio) respectively; Pour in 20 kg of water after shaking up and stirring; The all dissolvings in 2 minutes of this suspending agent, concrete dissolution time is as shown in table 3.The ratio when xanthan gum and ethanol water that experiment showed, restrains 24: in the time of below 100 milliliters, suspending agent dissolves in water comparatively fast, so preferred 3 ~ 24 gram xanthan gum: 100 milliliters ethanol water.
The relation of table 3 xanthan gum gram number and dissolution time
Get sodium carboxymethyl cellulose (CMC) 250 grams, also stir a small amount of slowly the adding in 100 kg of water, and its dissolution velocity is very slow, reaches tens hours.
The dissolution velocity result of two kinds of methods such as table 4 (dissolution velocity test), the dissolution velocity of this invention is the fastest.Mandatory declaration be; In the production of raising chickens on a large scale; Nearly 20,000 of the chicken numbers that each hen house is raised, if by 6 kilograms of every chicken drinking-water, contain suspending agent 0.25% and calculate (the present invention is not limited to 0.25%), the suspending agent that each hen house uses is a lot; Slowly adding will be wasted the plenty of time, influences the use of vaccine to chicken coccidiosis.
The test of table 4. dissolution velocity
Three, the suspending agent of this invention can make the vaccine egg capsule keep not sedimentation basically more than 8 hours, improves the uniformity that the vaccine egg capsule distributes.
Embodiment 5.
1, with xanthan gum 10.8 gram, 75 milliliters of mixing of 45% ethanol water as suspending agent, this suspending agent is poured in 18 kg of water and is stirred, this suspending agent dissolves very soon, this is the solution first.
2,18 kilograms in the water that does not add suspending agent.This is a second.
Get above-mentioned first, second solution each 100 milliliters with 1 bottle 2 milliliters same batch the chicken coccidiosis tetravalence live vaccine of totally 1000 plumage parts mix, shake up.After placing different time, take out 50 microlitres respectively from the upper strata, calculate the spore egg capsule quantity in this 50 microlitre with blood counting chamber.Result such as table 5. (cloud test test).
Can find out that from table 3 the egg capsule distribution consistency degree of solution first is good, can keep more than 8 hours constant basically.
The test of table 5. cloud test
Four, the suspending agent of this invention can be used to cut off the water immunity, can be used for the immunity of not cutting off the water again, can reduce the side reaction of vaccine and be successfully applied to the production of raising chickens.
This invention can suspend more than 8 hours the vaccine to chicken coccidiosis egg capsule in water, can realize chicken crowd's the immunity of not cutting off the water, and reduces stress; Also can be applied to the immunity of cutting off the water, shorten the operating time of immunity.Under two kinds of situation, can reduce the side reaction of vaccine.
After the side reaction of coccidial vaccine is meant vaccine immunity; The egg capsule of immunity is bred in the chicken body and is discharged more egg capsule of future generation, if immune chicken can not be resisted the attack of egg capsule of future generation, the coccidiosis symptom will appear in immune chicken; Even dead, have a strong impact on the effect of coccidiosis vaccine.So a kind of good coccidiosis vaccine must reduce the side reaction that causes owing to the coccidiosis vaccine immunity to greatest extent.
Two kinds of solution of first, second among 6.6 kilograms of embodiment 5 of embodiment respectively with the vaccine to chicken coccidiosis mixing of 1000 parts; Gave the immunity of 1000 chicken drinking-water after cutting off the water 2 hours; Raise on the rice husk bedding and padding immunity back; 20 chickens were dissected in immunity in back 12 days, according to the score of method record pathological changes (Johnson, the J. of Jhonson and Raid; And Reid; W.M.1970.Anticoccidial drugs:lesion scoring techniques in battery and floorpen experiments with chickens.Exp.parasital.28:30 ~ 36), this pathological changes score standard is listed in the last of the application's book, back 21 days of immunity; Get 10 chickens with 50,000 of the strong poison of tender eimeria tenella, 50,000 of the strong poison of murder by poisoning eimeria tenella, 50,000 of huge eimeria tenella virulent strain, 50,000 attacks of heap shape eimeria tenella virulent strain, dissect and write down the pathological changes score after 6 days.The average score at caecum, duodenum, three positions, small intestinal stage casing added be the score summation together.Result such as table 6 (the vaccine side reaction and the immune effect of the immunity of cutting off the water)..
Table 6. cut off the water the immunity vaccine side reaction and immune effect
The vaccine to chicken coccidiosis mixing that two kinds of solution of first, second among 7.6 kilograms of embodiment 5 of embodiment are produced with certain company of 1000 parts respectively; Under the situation of not cutting off the water, respectively give the immunity of 1000 chicken drinking-water; Raise on the rice husk bedding and padding immunity back; 20 chickens were dissected in immunity in back 13 days, according to the method record pathological changes score of Jhonson and Raid, back 21 days of immunity; Get 10 chickens with 50,000 of the strong poison of tender eimeria tenella, 50,000 of the strong poison of murder by poisoning eimeria tenella, 50,000 of huge eimeria tenella virulent strain, 50,000 attacks of heap shape eimeria tenella virulent strain, dissect and write down the pathological changes score after 6 days.The average score at caecum, duodenum, three positions, small intestinal stage casing added be the score summation together.Result such as table 7. (the vaccine side reaction and the immune effect of the immunity of not cutting off the water).
Table 7. do not cut off the water the immunity vaccine side reaction and immune effect
Can find out that from the result of table 6 and table 7 suspending agent of this invention can be used for the immunity of not cutting off the water, can be used to the immunity of cutting off the water again, obtain good immune effect; And under two kinds of situation, the intestinal pathological changes of the chicken of vaccine liquid first group immunity is all lighter, has reduced the side reaction of vaccine.
Chicken coccidiosis has been lived the egg capsule vaccine since the nineteen fifty-two appearance of COCCIVAC coccidiosis vaccine; The history in existing more than 50 year; In this very long process; Though the report that utilizes xanthan gum suspension chicken coccidial oocyst is arranged; But nobody utilizes drinking-water immunity that characteristic that xanthan gum that this invention mentions, mixture that ethanol water is formed can instant entry do not cut off the water to the chicken coccidiosis vaccine reducing drinking-water immunity after stress or cutting off the water shortening the operating time, and can both reduce the side reaction of vaccine under two kinds of situation.
Appendix 1 lesion score
After infecting, the pathological changes that 1 tender eimeria tenella causes cuts open inspection caecum (when the both sides caecum lesion is inconsistent, being as the criterion) between the 5th ~ 7 day with a serious side.
0 fen visible pathological changes of no naked eyes.
+ 1 fen caecum wall has and is dispersed in petechia or ecchymosis on a small quantity, and intestinal wall does not thicken, and intestinal contents is normal.
+ 2 fens petechias or ecchymosis quantity increase, and intestinal contents obviously is with blood, and intestinal wall slightly thickens, and the normal content thing is arranged
The caecum intracavity had a large amount of blood or caecum core (the cheesy Fructus Musae type of blood clot or canescence block) occurred in+3 minutes; The caecum wall extremely thickens; The caecum chamber does not have or only has a spot of caecum feces.
Caecum was extremely expanded because of amount of bleeding is how big with cheesy caecum core in+4 minutes; The caecum intracavity does not have feces or feces to be enclosed in the intestinal core; The chicken that dies of illness is designated as+and 4 minutes.
After infecting, the pathological changes that 2 huge eimeria tenellas cause cuts open the inspection small intestinal between the 6th ~ 7 day.
0 fen visible pathological changes of no naked eyes.
+ 1 fen small intestinal stage casing serous coat little red petechia of maying be seen indistinctly; Intestinal wall does not thicken, and has a small amount of orange colour mucus in the enteric cavity, does not have flatulence.
Serosal surface had many red petechias in+2 minutes; See the orange colour mucus of volume in the enteric cavity; Intestinal wall thickens; Slight flatulence or do not have flatulence.
There were flatulence and intestinal wall to thicken in+3 minutes; Mucosal surface is coarse; Be full of little blood clot and mucus in the intestinal contents.
+ 4 fens most of flatulence of small intestinal; Intestinal contents contains the erythrocyte of a large amount of blood clots and digestion and is bronzing; Intestinal wall significantly thickens.The chicken that dies of illness is designated as+and 4 minutes.
After infecting, the pathological changes that 3 heap shape eimeria tenellas cause cuts open the inspection duodenum between the 5th ~ 7 day.
0 fen visible pathological changes of no naked eyes.
The white tuberosity that is dispersed in that contained the egg capsule of growing in+1 minute is confined to duodenum; Tuberosity is microscler, and is transversely arranged, and it is trapezoidal that outward appearance is; Serosal surface and mucosal surface all can be seen tuberosity; Every square centimeter has 5 tuberositys at most.
Pathological changes was comparatively intensive in+2 minutes, but did not merge; 3 age in week the chick pathological changes possibly extend to 20 centimeters behind the duodenum; Intestinal wall does not thicken; Content is normal.
Pathological changes merged in flakes in+3 minutes, and intestinal wall seems to resemble and covered one deck pathological changes; Intestinal wall thickens, and content is water sample; Pathological changes may extend to the yolk stalk place.
+ 4 fens mucosal surface light grays, white tuberosity merges fully; Congested situation possibly show as little congestion point, and perhaps whole mucosal surface is cerise under severe infections extremely; Possibly can not differentiate single pathological changes at the small intestinal epimere; The visible typical trapezoidal pathological changes in the small intestinal stage casing; Intestinal wall extremely thickens, and is full of the butyrous exudate in the enteric cavity, has a large amount of egg capsules in the exudate; The chicken that dies of illness is designated as+and 4 minutes.
4, poisoning pathological changes that eimeria tenella causes infects the back and cuts open the inspection small intestinal between the 5th ~ 7 day.
0 fen visible pathological changes of no naked eyes.
+ 1 fen serosal surface is seen the petechia that is dispersed in and the white point of needle point size easily; Mucosal surface does not have or only has seldom pathological changes.
Serosal surface was bled profusely a little and white point in+2 minutes; The small intestinal stage casing may had slight flatulence.
Serosal surface was covered with red petechia and white point in+3 minutes; Mucosal surface is coarse, thicken, and many needle points sizes petechia is arranged; There is volume hemorrhage in the enteric cavity, do not have normal intestinal contents; Flatulence expands to whole small intestinal hypomere.
Bleeding profusely in+4 minutes makes small intestinal outward appearance purple darkly, contains red or brown mucus in the small intestine contents, and flatulence possibly expand to the small intestinal major part.The chicken that dies of illness is designated as 4 fens.
Claims (6)
1. a chicken coccidiosis vaccine suspending agent is characterized in that it is made up of xanthan gum and ethanol water, and wherein the volumetric concentration of ethanol water is 38% ~ 100%, and xanthan gum and ethanol water are 3% ~ 60% in the ratio of g/ml.
2. chicken coccidiosis vaccine suspending agent as claimed in claim 1 is characterized in that the volumetric concentration of said ethanol water is 45% ~ 100%.
3. chicken coccidiosis vaccine suspending agent as claimed in claim 2 is characterized in that the volumetric concentration of said ethanol water is 75%.
4. chicken coccidiosis vaccine suspending agent as claimed in claim 1 is characterized in that the ratio of xanthan gum and ethanol water is 3% ~ 24%.
5. chicken coccidiosis vaccine suspending agent as claimed in claim 1 is characterized in that, said for xanthan gum is food stage, pharmaceutical grade xanthan gum, said ethanol is food stage, pharmaceutical grade ethanol.
6. suspending liquid by each described suspending agent preparation of claim 1-5.
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| CN104885994A (en) * | 2015-05-04 | 2015-09-09 | 霍邱县科瑞达禽业有限公司 | Drinking water immunization method for chicken flock |
| CN108472351A (en) * | 2015-12-29 | 2018-08-31 | 英特维特国际股份有限公司 | Coccidiosis vaccine |
| CN110139670A (en) * | 2016-12-22 | 2019-08-16 | 英特维特国际股份有限公司 | Eimeria vaccine with improved effect |
| CN111330001A (en) * | 2020-03-24 | 2020-06-26 | 中国农业大学 | Rabbit coccidiosis attenuated live vaccine suspending agent and suspension |
| CN111979291A (en) * | 2020-08-13 | 2020-11-24 | 江苏京海禽业集团有限公司 | Method for detecting coccidian immune effect of AA + broiler breeder |
| CN116059331A (en) * | 2022-12-14 | 2023-05-05 | 佛山市正典生物技术有限公司 | Suspending agent granule and preparation method and application thereof |
| CN116531497A (en) * | 2023-05-09 | 2023-08-04 | 佛山市正典生物技术有限公司 | Method and kit of suspension products for rapid hydration of short rheology suspension |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104885994A (en) * | 2015-05-04 | 2015-09-09 | 霍邱县科瑞达禽业有限公司 | Drinking water immunization method for chicken flock |
| CN108472351A (en) * | 2015-12-29 | 2018-08-31 | 英特维特国际股份有限公司 | Coccidiosis vaccine |
| US11413343B2 (en) | 2015-12-29 | 2022-08-16 | Intervet Inc. | Coccidiosis vaccine |
| CN110139670A (en) * | 2016-12-22 | 2019-08-16 | 英特维特国际股份有限公司 | Eimeria vaccine with improved effect |
| CN110139670B (en) * | 2016-12-22 | 2023-10-31 | 英特维特国际股份有限公司 | Eimeria vaccine with improved potency |
| CN111330001A (en) * | 2020-03-24 | 2020-06-26 | 中国农业大学 | Rabbit coccidiosis attenuated live vaccine suspending agent and suspension |
| CN111979291A (en) * | 2020-08-13 | 2020-11-24 | 江苏京海禽业集团有限公司 | Method for detecting coccidian immune effect of AA + broiler breeder |
| CN116059331A (en) * | 2022-12-14 | 2023-05-05 | 佛山市正典生物技术有限公司 | Suspending agent granule and preparation method and application thereof |
| CN116059331B (en) * | 2022-12-14 | 2023-09-19 | 佛山市正典生物技术有限公司 | Suspending agent granule and preparation method and application thereof |
| CN116531497A (en) * | 2023-05-09 | 2023-08-04 | 佛山市正典生物技术有限公司 | Method and kit of suspension products for rapid hydration of short rheology suspension |
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