CN102719485A - Transformation method of ethanol by utilizing starch in momordica grosvenori swingle roots - Google Patents
Transformation method of ethanol by utilizing starch in momordica grosvenori swingle roots Download PDFInfo
- Publication number
- CN102719485A CN102719485A CN2012102235163A CN201210223516A CN102719485A CN 102719485 A CN102719485 A CN 102719485A CN 2012102235163 A CN2012102235163 A CN 2012102235163A CN 201210223516 A CN201210223516 A CN 201210223516A CN 102719485 A CN102719485 A CN 102719485A
- Authority
- CN
- China
- Prior art keywords
- momordica
- grosvenor momordica
- add
- ethanol
- starch
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 title claims abstract description 86
- 229920002472 Starch Polymers 0.000 title claims abstract description 28
- 239000008107 starch Substances 0.000 title claims abstract description 28
- 235000019698 starch Nutrition 0.000 title claims abstract description 28
- 235000011171 Thladiantha grosvenorii Nutrition 0.000 title abstract description 33
- 241001409321 Siraitia grosvenorii Species 0.000 title abstract 4
- 238000011426 transformation method Methods 0.000 title abstract 2
- 238000000034 method Methods 0.000 claims abstract description 68
- 230000008569 process Effects 0.000 claims abstract description 16
- 235000009815 Momordica Nutrition 0.000 claims description 77
- 241000218984 Momordica Species 0.000 claims description 77
- 239000007788 liquid Substances 0.000 claims description 25
- 239000000047 product Substances 0.000 claims description 21
- 238000000855 fermentation Methods 0.000 claims description 16
- 230000004151 fermentation Effects 0.000 claims description 16
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 12
- 240000006439 Aspergillus oryzae Species 0.000 claims description 11
- 235000002247 Aspergillus oryzae Nutrition 0.000 claims description 11
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 claims description 10
- 230000001476 alcoholic effect Effects 0.000 claims description 9
- 230000015556 catabolic process Effects 0.000 claims description 8
- 238000006731 degradation reaction Methods 0.000 claims description 8
- 108010059892 Cellulase Proteins 0.000 claims description 7
- 239000002253 acid Substances 0.000 claims description 6
- 229940106157 cellulase Drugs 0.000 claims description 6
- 235000019270 ammonium chloride Nutrition 0.000 claims description 5
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 5
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 5
- 235000019797 dipotassium phosphate Nutrition 0.000 claims description 5
- 239000000413 hydrolysate Substances 0.000 claims description 5
- 230000003068 static effect Effects 0.000 claims description 5
- 238000006243 chemical reaction Methods 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 13
- 230000002829 reductive effect Effects 0.000 abstract description 9
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 6
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 abstract description 4
- 239000002699 waste material Substances 0.000 abstract description 4
- 230000008901 benefit Effects 0.000 abstract description 3
- 238000005406 washing Methods 0.000 abstract description 3
- 231100000252 nontoxic Toxicity 0.000 abstract description 2
- 230000003000 nontoxic effect Effects 0.000 abstract description 2
- 239000003208 petroleum Substances 0.000 abstract description 2
- 238000011084 recovery Methods 0.000 abstract description 2
- 239000000284 extract Substances 0.000 description 52
- 238000000605 extraction Methods 0.000 description 30
- 244000185386 Thladiantha grosvenorii Species 0.000 description 29
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 27
- 239000012141 concentrate Substances 0.000 description 23
- 235000008504 concentrate Nutrition 0.000 description 22
- 239000011347 resin Substances 0.000 description 20
- 229920005989 resin Polymers 0.000 description 20
- 239000002994 raw material Substances 0.000 description 17
- 239000000243 solution Substances 0.000 description 17
- 238000001035 drying Methods 0.000 description 16
- 229930182470 glycoside Natural products 0.000 description 14
- 150000002338 glycosides Chemical class 0.000 description 14
- 235000019198 oils Nutrition 0.000 description 13
- 238000002360 preparation method Methods 0.000 description 13
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 12
- 238000010992 reflux Methods 0.000 description 11
- 238000001914 filtration Methods 0.000 description 10
- 241000196324 Embryophyta Species 0.000 description 9
- 238000000926 separation method Methods 0.000 description 9
- 239000002904 solvent Substances 0.000 description 9
- GHBNZZJYBXQAHG-KUVSNLSMSA-N (2r,3r,4s,5s,6r)-2-[[(2r,3s,4s,5r,6r)-6-[[(3s,8s,9r,10r,11r,13r,14s,17r)-17-[(2r,5r)-5-[(2s,3r,4s,5s,6r)-4,5-dihydroxy-3-[(2r,3r,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-6-[[(2r,3r,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy Chemical compound C([C@H]1O[C@H]([C@@H]([C@@H](O)[C@@H]1O)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)O[C@H](CC[C@@H](C)[C@@H]1[C@]2(C[C@@H](O)[C@@]3(C)[C@H]4C(C([C@@H](O[C@H]5[C@@H]([C@@H](O)[C@H](O)[C@@H](CO[C@H]6[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O6)O)O5)O)CC4)(C)C)=CC[C@H]3[C@]2(C)CC1)C)C(C)(C)O)O[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O GHBNZZJYBXQAHG-KUVSNLSMSA-N 0.000 description 8
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 8
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 8
- 239000012535 impurity Substances 0.000 description 8
- 239000012528 membrane Substances 0.000 description 8
- TVJXHJAWHUMLLG-UHFFFAOYSA-N mogroside V Natural products CC(CCC(OC1OC(COC2OC(CO)C(O)C(O)C2OC3OC(CO)C(O)C(O)C3O)C(O)C(O)C1O)C(C)(C)O)C4CCC5(C)C6CC=C7C(CCC(OC8OC(COC9OC(CO)C(O)C(O)C9O)C(O)C(O)C8O)C7(C)C)C6(C)C(O)CC45C TVJXHJAWHUMLLG-UHFFFAOYSA-N 0.000 description 8
- 235000013399 edible fruits Nutrition 0.000 description 7
- 238000010828 elution Methods 0.000 description 7
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 description 6
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 6
- -1 Flavonoid compound Chemical class 0.000 description 6
- 238000010521 absorption reaction Methods 0.000 description 6
- 239000001913 cellulose Substances 0.000 description 6
- 229920002678 cellulose Polymers 0.000 description 6
- 239000003456 ion exchange resin Substances 0.000 description 6
- 229920003303 ion-exchange polymer Polymers 0.000 description 6
- 239000000203 mixture Substances 0.000 description 6
- 238000000746 purification Methods 0.000 description 6
- TWCMVXMQHSVIOJ-UHFFFAOYSA-N Aglycone of yadanzioside D Natural products COC(=O)C12OCC34C(CC5C(=CC(O)C(O)C5(C)C3C(O)C1O)C)OC(=O)C(OC(=O)C)C24 TWCMVXMQHSVIOJ-UHFFFAOYSA-N 0.000 description 5
- PLMKQQMDOMTZGG-UHFFFAOYSA-N Astrantiagenin E-methylester Natural products CC12CCC(O)C(C)(CO)C1CCC1(C)C2CC=C2C3CC(C)(C)CCC3(C(=O)OC)CCC21C PLMKQQMDOMTZGG-UHFFFAOYSA-N 0.000 description 5
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 5
- 239000003463 adsorbent Substances 0.000 description 5
- 235000013339 cereals Nutrition 0.000 description 5
- 239000000470 constituent Substances 0.000 description 5
- 230000003247 decreasing effect Effects 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 5
- 229930003935 flavonoid Natural products 0.000 description 5
- 235000017173 flavonoids Nutrition 0.000 description 5
- 235000015203 fruit juice Nutrition 0.000 description 5
- 150000004676 glycans Chemical class 0.000 description 5
- PFOARMALXZGCHY-UHFFFAOYSA-N homoegonol Natural products C1=C(OC)C(OC)=CC=C1C1=CC2=CC(CCCO)=CC(OC)=C2O1 PFOARMALXZGCHY-UHFFFAOYSA-N 0.000 description 5
- 239000004615 ingredient Substances 0.000 description 5
- 238000007670 refining Methods 0.000 description 5
- 238000005507 spraying Methods 0.000 description 5
- 150000003648 triterpenes Chemical class 0.000 description 5
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 4
- 240000006053 Garcinia mangostana Species 0.000 description 4
- 235000017048 Garcinia mangostana Nutrition 0.000 description 4
- FEWJPZIEWOKRBE-UHFFFAOYSA-N Tartaric acid Natural products [H+].[H+].[O-]C(=O)C(O)C(O)C([O-])=O FEWJPZIEWOKRBE-UHFFFAOYSA-N 0.000 description 4
- 238000004587 chromatography analysis Methods 0.000 description 4
- 238000004140 cleaning Methods 0.000 description 4
- 230000008021 deposition Effects 0.000 description 4
- 235000013305 food Nutrition 0.000 description 4
- 235000021022 fresh fruits Nutrition 0.000 description 4
- 229930189775 mogroside Natural products 0.000 description 4
- 239000012071 phase Substances 0.000 description 4
- 229920001282 polysaccharide Polymers 0.000 description 4
- 239000005017 polysaccharide Substances 0.000 description 4
- 239000000843 powder Substances 0.000 description 4
- 229930182490 saponin Natural products 0.000 description 4
- 235000017709 saponins Nutrition 0.000 description 4
- 238000000526 short-path distillation Methods 0.000 description 4
- 238000001179 sorption measurement Methods 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 235000002906 tartaric acid Nutrition 0.000 description 4
- 239000011975 tartaric acid Substances 0.000 description 4
- 235000015112 vegetable and seed oil Nutrition 0.000 description 4
- CRDAMVZIKSXKFV-FBXUGWQNSA-N (2-cis,6-cis)-farnesol Chemical compound CC(C)=CCC\C(C)=C/CC\C(C)=C/CO CRDAMVZIKSXKFV-FBXUGWQNSA-N 0.000 description 3
- 239000000260 (2E,6E)-3,7,11-trimethyldodeca-2,6,10-trien-1-ol Substances 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 230000008030 elimination Effects 0.000 description 3
- 238000003379 elimination reaction Methods 0.000 description 3
- 229940088598 enzyme Drugs 0.000 description 3
- 229940043259 farnesol Drugs 0.000 description 3
- 229930002886 farnesol Natural products 0.000 description 3
- 239000000706 filtrate Substances 0.000 description 3
- 229930003944 flavone Natural products 0.000 description 3
- 150000002213 flavones Chemical class 0.000 description 3
- 235000011949 flavones Nutrition 0.000 description 3
- 150000002215 flavonoids Chemical class 0.000 description 3
- 238000013467 fragmentation Methods 0.000 description 3
- 238000006062 fragmentation reaction Methods 0.000 description 3
- 238000010438 heat treatment Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 235000012054 meals Nutrition 0.000 description 3
- 239000012452 mother liquor Substances 0.000 description 3
- 238000010898 silica gel chromatography Methods 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- CRDAMVZIKSXKFV-UHFFFAOYSA-N trans-Farnesol Natural products CC(C)=CCCC(C)=CCCC(C)=CCO CRDAMVZIKSXKFV-UHFFFAOYSA-N 0.000 description 3
- 238000000108 ultra-filtration Methods 0.000 description 3
- 238000003809 water extraction Methods 0.000 description 3
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- 241000345998 Calamus manan Species 0.000 description 2
- 238000007445 Chromatographic isolation Methods 0.000 description 2
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 2
- 229930091371 Fructose Natural products 0.000 description 2
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 2
- 239000005715 Fructose Substances 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 240000003183 Manihot esculenta Species 0.000 description 2
- 235000016735 Manihot esculenta subsp esculenta Nutrition 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 229930193416 Siraitic acid Natural products 0.000 description 2
- NIXOWILDQLNWCW-UHFFFAOYSA-N acrylic acid group Chemical group C(C=C)(=O)O NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 239000003513 alkali Substances 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 230000000975 bioactive effect Effects 0.000 description 2
- 235000019658 bitter taste Nutrition 0.000 description 2
- 238000009835 boiling Methods 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000011097 chromatography purification Methods 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 235000009508 confectionery Nutrition 0.000 description 2
- 230000006837 decompression Effects 0.000 description 2
- 239000008367 deionised water Substances 0.000 description 2
- 229910021641 deionized water Inorganic materials 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000003810 ethyl acetate extraction Methods 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 239000000796 flavoring agent Substances 0.000 description 2
- 235000019634 flavors Nutrition 0.000 description 2
- 235000003599 food sweetener Nutrition 0.000 description 2
- 238000005194 fractionation Methods 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 229930182478 glucoside Natural products 0.000 description 2
- 150000008131 glucosides Chemical class 0.000 description 2
- 230000007062 hydrolysis Effects 0.000 description 2
- 238000006460 hydrolysis reaction Methods 0.000 description 2
- 239000006166 lysate Substances 0.000 description 2
- 238000001471 micro-filtration Methods 0.000 description 2
- 239000012046 mixed solvent Substances 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 238000001728 nano-filtration Methods 0.000 description 2
- 239000001814 pectin Substances 0.000 description 2
- 229920001277 pectin Polymers 0.000 description 2
- 235000010987 pectin Nutrition 0.000 description 2
- 229920006122 polyamide resin Polymers 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 239000001397 quillaja saponaria molina bark Substances 0.000 description 2
- 235000012950 rattan cane Nutrition 0.000 description 2
- 238000001953 recrystallisation Methods 0.000 description 2
- 238000004064 recycling Methods 0.000 description 2
- 150000008265 rhamnosides Chemical class 0.000 description 2
- 150000007949 saponins Chemical class 0.000 description 2
- 239000000741 silica gel Substances 0.000 description 2
- 229910002027 silica gel Inorganic materials 0.000 description 2
- 229960001866 silicon dioxide Drugs 0.000 description 2
- 238000000638 solvent extraction Methods 0.000 description 2
- 239000003765 sweetening agent Substances 0.000 description 2
- LTDANPHZAHSOBN-IPIJHGFVSA-N (2R,3R,4S,5S,6R)-2-[[(2R,3S,4S,5R,6R)-6-[[(3S,8R,9R,10S,11R,13R,14S,17R)-17-[(2R,5R)-5-[(2S,3R,4S,5S,6R)-4,5-dihydroxy-3-[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-6-[[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxymethyl]oxan-2-yl]oxy-6-hydroxy-6-methylheptan-2-yl]-11-hydroxy-4,4,9,13,14-pentamethyl-2,3,7,8,10,11,12,15,16,17-decahydro-1H-cyclopenta[a]phenanthren-3-yl]oxy]-3,4-dihydroxy-5-[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyoxan-2-yl]methoxy]-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound C([C@H]1O[C@H]([C@@H]([C@@H](O)[C@@H]1O)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)O[C@H](CC[C@@H](C)[C@@H]1[C@]2(C[C@@H](O)[C@@]3(C)[C@@H]4C(C([C@@H](O[C@H]5[C@@H]([C@@H](O)[C@H](O)[C@@H](CO[C@H]6[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O6)O)O5)O[C@H]5[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O5)O)CC4)(C)C)=CC[C@@H]3[C@]2(C)CC1)C)C(C)(C)O)O[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O LTDANPHZAHSOBN-IPIJHGFVSA-N 0.000 description 1
- WRPAFPPCKSYACJ-ZBYJYCAASA-N (2r,3r,4s,5s,6r)-2-[[(2r,3s,4s,5r,6r)-6-[[(3s,8r,9r,10s,11r,13r,14s,17r)-17-[(5r)-5-[(2s,3r,4s,5s,6r)-4,5-dihydroxy-6-(hydroxymethyl)-3-[(2r,3s,4r,5r,6s)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyoxan-2-yl]oxy-6-hydroxy-6-methylheptan-2-yl]-11-hydrox Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@H](CCC(C)[C@@H]1[C@]2(C[C@@H](O)[C@@]3(C)[C@@H]4C(C([C@@H](O[C@H]5[C@@H]([C@@H](O)[C@H](O)[C@@H](CO[C@H]6[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O6)O)O5)O)CC4)(C)C)=CC[C@@H]3[C@]2(C)CC1)C)C(C)(C)O)[C@H]1O[C@@H](CO)[C@H](O)[C@@H](O)[C@@H]1O WRPAFPPCKSYACJ-ZBYJYCAASA-N 0.000 description 1
- KYVIPFHNYCKOMQ-YMRJDYICSA-N (2r,3s,4s,5r,6r)-2-(hydroxymethyl)-6-[[(2r,3s,4s,5r,6s)-3,4,5-trihydroxy-6-[(3r,6r)-2-hydroxy-6-[(3s,8s,9r,10r,11r,13r,14s,17r)-11-hydroxy-4,4,9,13,14-pentamethyl-3-[(2r,3r,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-2,3,7,8,10,11,12,15,16,1 Chemical compound C([C@H]1O[C@H]([C@@H]([C@@H](O)[C@@H]1O)O)O[C@H](CC[C@@H](C)[C@@H]1[C@]2(C[C@@H](O)[C@@]3(C)[C@H]4C(C([C@@H](O[C@H]5[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O5)O)CC4)(C)C)=CC[C@H]3[C@]2(C)CC1)C)C(C)(C)O)O[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O KYVIPFHNYCKOMQ-YMRJDYICSA-N 0.000 description 1
- 229930192771 11-oxomogroside Natural products 0.000 description 1
- QATISCJMIITVAB-UHFFFAOYSA-N 2-[[17-[5-[4,5-dihydroxy-6-(hydroxymethyl)-3-[3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyoxan-2-yl]oxy-6-hydroxy-6-methylheptan-2-yl]-11-hydroxy-4,4,9,13,14-pentamethyl-2,3,7,8,10,11,12,15,16,17-decahydro-1h-cyclopenta[a]phenanthren-3-yl]oxy]-6-(hydro Chemical compound C1CC2(C)C3CC=C(C(C(OC4C(C(O)C(O)C(CO)O4)O)CC4)(C)C)C4C3(C)C(O)CC2(C)C1C(C)CCC(C(C)(C)O)OC1OC(CO)C(O)C(O)C1OC1OC(CO)C(O)C(O)C1O QATISCJMIITVAB-UHFFFAOYSA-N 0.000 description 1
- 238000005084 2D-nuclear magnetic resonance Methods 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- SHZGCJCMOBCMKK-UHFFFAOYSA-N D-mannomethylose Natural products CC1OC(O)C(O)C(O)C1O SHZGCJCMOBCMKK-UHFFFAOYSA-N 0.000 description 1
- 206010013786 Dry skin Diseases 0.000 description 1
- 244000017020 Ipomoea batatas Species 0.000 description 1
- 235000002678 Ipomoea batatas Nutrition 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- WVXIMWMLKSCVTD-JLRHFDOOSA-N Mogroside II-E Chemical compound O([C@H](CC[C@@H](C)[C@@H]1[C@]2(C[C@@H](O)[C@@]3(C)[C@H]4C(C([C@@H](O[C@H]5[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O5)O)CC4)(C)C)=CC[C@H]3[C@]2(C)CC1)C)C(C)(C)O)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O WVXIMWMLKSCVTD-JLRHFDOOSA-N 0.000 description 1
- KYVIPFHNYCKOMQ-UHFFFAOYSA-N Mogroside III Natural products C1CC2(C)C3CC=C(C(C(OC4C(C(O)C(O)C(CO)O4)O)CC4)(C)C)C4C3(C)C(O)CC2(C)C1C(C)CCC(C(C)(C)O)OC(C(C(O)C1O)O)OC1COC1OC(CO)C(O)C(O)C1O KYVIPFHNYCKOMQ-UHFFFAOYSA-N 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 1
- 241000218689 Podocarpus Species 0.000 description 1
- 239000004952 Polyamide Substances 0.000 description 1
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 1
- YWPVROCHNBYFTP-UHFFFAOYSA-N Rubusoside Natural products C1CC2C3(C)CCCC(C)(C(=O)OC4C(C(O)C(O)C(CO)O4)O)C3CCC2(C2)CC(=C)C21OC1OC(CO)C(O)C(O)C1O YWPVROCHNBYFTP-UHFFFAOYSA-N 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- 238000005903 acid hydrolysis reaction Methods 0.000 description 1
- 239000003929 acidic solution Substances 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000000274 adsorptive effect Effects 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000003011 anion exchange membrane Substances 0.000 description 1
- 150000001450 anions Chemical class 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 239000002246 antineoplastic agent Substances 0.000 description 1
- 229940041181 antineoplastic drug Drugs 0.000 description 1
- 239000008346 aqueous phase Substances 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000000712 assembly Effects 0.000 description 1
- 238000000429 assembly Methods 0.000 description 1
- 235000013405 beer Nutrition 0.000 description 1
- WPYMKLBDIGXBTP-UHFFFAOYSA-N benzoic acid Chemical compound OC(=O)C1=CC=CC=C1 WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 description 1
- 229940088623 biologically active substance Drugs 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 125000002091 cationic group Chemical group 0.000 description 1
- WORJEOGGNQDSOE-UHFFFAOYSA-N chloroform;methanol Chemical compound OC.ClC(Cl)Cl WORJEOGGNQDSOE-UHFFFAOYSA-N 0.000 description 1
- SIHHLZPXQLFPMC-UHFFFAOYSA-N chloroform;methanol;hydrate Chemical compound O.OC.ClC(Cl)Cl SIHHLZPXQLFPMC-UHFFFAOYSA-N 0.000 description 1
- 235000019504 cigarettes Nutrition 0.000 description 1
- 238000005352 clarification Methods 0.000 description 1
- 239000003245 coal Substances 0.000 description 1
- 238000010960 commercial process Methods 0.000 description 1
- 239000000287 crude extract Substances 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 238000004042 decolorization Methods 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000004332 deodorization Methods 0.000 description 1
- 238000010612 desalination reaction Methods 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000012869 ethanol precipitation Methods 0.000 description 1
- 150000002191 fatty alcohols Chemical class 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 229930182486 flavonoid glycoside Natural products 0.000 description 1
- 150000007955 flavonoid glycosides Chemical class 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 239000012510 hollow fiber Substances 0.000 description 1
- WGCNASOHLSPBMP-UHFFFAOYSA-N hydroxyacetaldehyde Natural products OCC=O WGCNASOHLSPBMP-UHFFFAOYSA-N 0.000 description 1
- 201000001421 hyperglycemia Diseases 0.000 description 1
- 230000002218 hypoglycaemic effect Effects 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 239000003014 ion exchange membrane Substances 0.000 description 1
- 235000014666 liquid concentrate Nutrition 0.000 description 1
- 239000006210 lotion Substances 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 230000028161 membrane depolarization Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000000874 microwave-assisted extraction Methods 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- WVXIMWMLKSCVTD-UHFFFAOYSA-N mogroside II E Natural products C1CC2(C)C3CC=C(C(C(OC4C(C(O)C(O)C(CO)O4)O)CC4)(C)C)C4C3(C)C(O)CC2(C)C1C(C)CCC(C(C)(C)O)OC1OC(CO)C(O)C(O)C1O WVXIMWMLKSCVTD-UHFFFAOYSA-N 0.000 description 1
- 229930191869 mogroside IV Natural products 0.000 description 1
- OKGRRPCHOJYNKX-UHFFFAOYSA-N mogroside IV A Natural products C1CC2(C)C3CC=C(C(C(OC4C(C(O)C(O)C(COC5C(C(O)C(O)C(CO)O5)O)O4)O)CC4)(C)C)C4C3(C)C(O)CC2(C)C1C(C)CCC(C(C)(C)O)OC(C(C(O)C1O)O)OC1COC1OC(CO)C(O)C(O)C1O OKGRRPCHOJYNKX-UHFFFAOYSA-N 0.000 description 1
- WRPAFPPCKSYACJ-UHFFFAOYSA-N mogroside IV E Natural products C1CC2(C)C3CC=C(C(C(OC4C(C(O)C(O)C(COC5C(C(O)C(O)C(CO)O5)O)O4)O)CC4)(C)C)C4C3(C)C(O)CC2(C)C1C(C)CCC(C(C)(C)O)OC1OC(CO)C(O)C(O)C1OC1OC(CO)C(O)C(O)C1O WRPAFPPCKSYACJ-UHFFFAOYSA-N 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 239000012454 non-polar solvent Substances 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 239000003895 organic fertilizer Substances 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- TWNQGVIAIRXVLR-UHFFFAOYSA-N oxo(oxoalumanyloxy)alumane Chemical compound O=[Al]O[Al]=O TWNQGVIAIRXVLR-UHFFFAOYSA-N 0.000 description 1
- 238000009928 pasteurization Methods 0.000 description 1
- 229920002647 polyamide Polymers 0.000 description 1
- 150000008442 polyphenolic compounds Chemical class 0.000 description 1
- 235000013824 polyphenols Nutrition 0.000 description 1
- 235000012015 potatoes Nutrition 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 238000001223 reverse osmosis Methods 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- YWPVROCHNBYFTP-OSHKXICASA-N rubusoside Chemical compound O([C@]12C(=C)C[C@@]3(C1)CC[C@@H]1[C@@](C)(CCC[C@]1([C@@H]3CC2)C)C(=O)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O YWPVROCHNBYFTP-OSHKXICASA-N 0.000 description 1
- 230000002000 scavenging effect Effects 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 239000002893 slag Substances 0.000 description 1
- 239000002594 sorbent Substances 0.000 description 1
- 235000014347 soups Nutrition 0.000 description 1
- 238000004611 spectroscopical analysis Methods 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 238000010025 steaming Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 235000019605 sweet taste sensations Nutrition 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
- 235000019640 taste Nutrition 0.000 description 1
- 239000010409 thin film Substances 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 229940126680 traditional chinese medicines Drugs 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 230000001052 transient effect Effects 0.000 description 1
- 238000002137 ultrasound extraction Methods 0.000 description 1
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 1
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 1
- 238000001291 vacuum drying Methods 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
- 238000009736 wetting Methods 0.000 description 1
- 235000015099 wheat brans Nutrition 0.000 description 1
- 239000001052 yellow pigment Substances 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
A transformation method of ethanol by utilizing starch in momordica grosvenori swingle roots includes washing the momordica grosvenori swingle roots, cutting the roots into slices, grinding, extracting petroleum ether, saccharifying and filtrating, biologically fermentating and distillating to obtain the finished product of the ethanol. The method has the advantages that the operation is simple, production cost of the ethanol is greatly reduced, production process is simplified, utilization rate of the starch in momordica grosvenori swingle roots is greatly improved, and starch resource is saved. Reagents used in the method are non-toxic, cheap and mass produced chemical reagents. Mature reagent recovery routine techniques can be used in the whole process, and wastes discharged into environments are greatly reduced.
Description
Technical field
The present invention relates to a kind of starch conversion alcoholic acid method in the Grosvenor Momordica piece root of utilizing, more particularly having related to the microbial transformation of a kind of direct employing Grosvenor Momordica piece root is the alcoholic acid method.
Background technology
Based on the present situation of China's national situation and alcohol industry, alcohol industry is faced with the challenge from 3 problems such as raw material, production cost, process for cleanly preparing at present.In the past few years, it is cereal grain-production alcohol that China utilizes grain in storage for years in a large number, still, it should be noted that China is populous, and per capita area of cultivated farmland is less, and the grain residue is transient phenomena just.Receive the restriction of cultivated area with the sweet potato for raw material production alcohol, and be raw material with the cassava, at China's depolarization individually outside the province, the complete dependence on import of cassava, cost is high, and is unrealistic.
Select for use Grosvenor Momordica piece root as the raw material of producing alcohol, the one, the production alcohol that meets National Development and Reform Committee's proposition should be accomplished not strive grain with the people, does not strive the principles and policies on ground with grain; The 2nd, starch content is high in the Grosvenor Momordica piece root; The 3rd, Grosvenor Momordica piece root does not fully obtain utilizing at present mainly as offal treatment.
Obtain materials such as mogroside V, mogroside IV, mogroside III, mogroside II E, mogroside III E, mogroside VI, mogroside A, the new glucoside of Grosvenor Momordica, Simon glucoside I, 11-oxidation mogroside 11-oxomogroside, Grosvenor Momordica glycol benzoate, amino acid, kaempferol-3,7-α-L two rhamnosides (II), Grosvenor Momordica element, unsaturated fatty acids, farnesol, N.F,USP MANNITOL, arhat tartaric acid first, arhat tartaric acid second, arhat tartaric acid third, arhat tartaric acid fourth existing main from the fruit of Grosvenor Momordica, the separation.
Publication number is that the Chinese patent of CN1397539 has been introduced a kind of method of from the Grosvenor Momordica residue, extracting multiple effective ingredient: the residue to extract behind the Momordica-Glycosides is a raw material; Through extracting seed oil; Extraction, fractionation obtain farnesol from seed oil again; Extract again from the aqueous phase of seed oil extraction and to obtain alkanoic, make food fibre with chemical method or enzyme process from cot or the mixing slag after extracting seed oil.Method of the present invention is simple and convenient, and yield is high, for new raw material sources have been developed in the production of farnesol, also the Grosvenor Momordica industry is brought great promoter action simultaneously, and good economic benefit and social benefit are arranged.
Publication number is that the Chinese patent of CN1436786 has been introduced a kind of method of from Grosvenor Momordica, extracting Momordica-Glycosides: with the fresh Fructus Momordicae is raw material; With water is extraction solvent; Adopting micro-filtration, ultrafiltration, nanofiltration is main extraction process, and wherein microfiltration membrane adopts the millipore filtration in 0.05 μ m～0.3 μ m aperture; It is 20000～80000 hollow fiber ultrafiltration membrane that ultra-filtration membrane adopts the relative molecular weight that dams; It is that 100～400 nf membrane or RO reverse osmosis equipment concentrate that the relative molecular weight that dams is adopted in nanofiltration.
Publication number is the process for extracting that the Chinese patent of CN87101850 has been introduced a kind of mangosteen sweetener: grind, pull an oar with mangosteen fruit; Warm water 50-60 ℃ extraction is filtered. separate 50% (V/V) dissolve with ethanol through DA-201 acrylic type sorbent material; Through the decolouring of D-211 acrylic type negative resin, desalination, decon. distillation method reclaims ethanol; Through the vacuum pump concentrating under reduced pressure, lyophilize promptly gets white crystals shape mangosteen sweetener.
Publication number is that the Chinese patent of CN1181908 has been introduced a kind of production process of fresh concentrate podocarpus fruit juice: with screw press crusher machine fresh Fructus Momordicae; With the level Four digesting technoloy with breaked fresh Fructus Momordicae boiling lixiviate get juice, the utilization prozyme makes the pectin enzymolysis deposition in the fruit juice that boiling gets; Make juice clarification, with sheet frame zeyssatite pressure filter the fruit juice after clarifying filtered then; Again with flash pasteurization with fruit juice sterilization and with centrifugal thin-film method and vacuum concentration pan with concentration of juices to 25Be, make finished product be cooled to normal temperature at last and pack.
Publication number is that the Chinese patent of CN1611498 has been introduced a kind of grosvenor momordica flavonoid and preparation technology thereof: utilize water or ethanol to be solvent; Adopt the refluxing extraction method; Flavonoid compound is extracted from the leaf of Grosvenor Momordica and rattan; Adopt the resin absorption method with the flavonoid compound enrichment in the extracting solution again, again with the ethanolic soln wash-out, concentrate, dry, make and contain the flavones amount greater than 25% bullion; With bullion again with alcohol extraction, filtration, concentrate, dry, promptly make flavones content greater than 50% elaboration grosvenor momordica flavonoid.
Publication number is the preparation method that the Chinese patent of CN1634814 has been introduced a kind of Grosvenor Momordica triterpene: the Grosvenor Momordica triterpene that will from fresh Fructus Momordicae, extract earlier places to be deposited more than 3 years under the normal temperature; Make its natural alcoholization; Grosvenor Momordica triterpene after will refining then places-10～10 ℃ to take out after freezing 5～40 minutes down; Place again under 80～120 ℃ after it is thawed it at normal temperatures, heat and take out cool the getting final product of drying in the air at normal temperatures after 5～40 minutes.
Publication number is that the Chinese patent of CN1508139 has been introduced a kind of process for extracting rubusoside of fruit of Gorsvenor Momordica: Grosvenor Momordica is broken, add ethanolic soln, and under 30～60 ℃ of temperature, soaked 5～15 hours.Cross and filter filtrating, temperature is reduced to 20～35 ℃, and filtrating is sent into three kinds of membrane separation assemblies through pressure pump order under 0.01～0.2MPa WP; The first step is held back and is isolated spissated Grosvenor Momordica fiber, protein, pectin and impurity, is used to make organic fertilizer; The second stage is spissated Momordica-Glycosides solution, through vacuum-drying, gets the Momordica-Glycosides product; The third stage is spissated momordica grosvenori polysaccharide and trace element, is the Grosvenor Momordica massecuite; The residual filtrate recycling use.
Publication number is that the Chinese patent of CN1683384 has been introduced a kind of fresh momordica glycoside process for extracting and equipment: fresh Fructus Momordicae is cleaned broken juice; Place the high voltage precision filtering separation device to handle in the fresh Fructus Momordicae juice of gained; The fresh Fructus Momordicae liquid concentrator of gained is dry, promptly gets fresh momordica glycoside.Method of the present invention is to adopt the equipment of Purely mechanical that the sweet glycosides of bright arhat is extracted; The extraction equipment of squeezing the juice that is wherein adopted only can be smashed the fresh Fructus Momordicae pulp; Can't its kernel together be smashed; Make the mogroside that is extracted can not contain the composition and the taste of Grosvenor Momordica kernel, can obtain highly purified Grosvenor Momordica juice.
Publication number is that the Chinese patent of CN1683387 has been introduced a kind of method of from Grosvenor Momordica, extracting Momordica-Glycosides: with the Grosvenor Momordica is raw material, and through the method that extraction solvent extracts, concentrates, Momordica-Glycosides is extracted in separation and purification, it is a solvent with water or low-carbon alcohol; The method of extracting is water heating and extracting or ultrasonic extraction or microwave extraction; Separation and purification is column chromatographic isolation and purification or membrane separation purification, in the extract Momordica-Glycosides weight content be 40～80% or the Momordica grosvenori mogroside V weight content be 20～40%.
Publication number is preparation method and the purposes that the Chinese patent of CN1706861 has been introduced a kind of high-purity momordica glycoside V: earlier Grosvenor Momordica is pulverized, extracted with alcohol heating reflux, extract is separated with D101 type macroporous resin column again; Elder generation's deionized water and ethanol are eluted to colourless respectively, regather its elutriant, are evaporated to the medicinal extract shape; Separate with normal phase silicagel column then, adding chloroform, methyl alcohol, water are eluent, collect elutriant; Concentrating under reduced pressure; Separate with reverse phase silica gel C-18 post again, and utilize HPLC to detect collected each part elutriant, merge the identical and purity of RT between 90%～98% with momordica glycoside V component more than 98%; Concentrating under reduced pressure can obtain momordica glycoside V and the purity of purity between 90%～98% respectively greater than 98% momordica glycoside V component.
Publication number is the process for extracting that the Chinese patent of CN1854149 has been introduced a kind of Momordica grosvenori mogroside V: with the Grosvenor Momordica fresh fruit is raw material; After cleaning, fragmentation, poach extract; Reconcentration poach extracting solution carries out fractionation by adsorption with macroporous ion-exchange resin, macroporous ion-exchange resin is carried out gradient clean; Final scavenging solution is carried out decompression recycling ethanol get medicinal extract, powdered extract can obtain product of the present invention.Weight with the Grosvenor Momordica fresh fruit is radix, and the output capacity of extract can reach more than 1.1%, and in the extract total amount, the content of Momordica grosvenori mogroside V can reach 30%-65%.
Publication number is the process for extracting that the Chinese patent of CN101100481 has been introduced a kind of fructus momordicae tetranuclear triterpene: the yellow pigment in the first warm water lixiviate fresh Fructus Momordicae pericarp; Reheat makes the gluey curing of the intracellular protein of Lo Han Guo fruit can not stripping; Broken then; Use 60-90 ℃ of hot water lixiviate again, vat liquor is through pectase degumming, filters, concentrates, drying obtains the tetracyclic triterpene bullion.
Publication number is that the Chinese patent of CN101327244 has been introduced a kind of decolouring, debitterized Momordica grosvenori extract and preparation method thereof: Grosvenor Momordica cleaned, and broken juice, fruit juice is centrifugal; Supernatant advances macroporous adsorbent resin, and water, basic solvent and washed resin are successively washed to effluent and be neutral; Use 30%～65% ethanol elution again, collect ethanol eluate, reclaim ethanol; Drying promptly gets.
Publication number is the process for extracting that the Chinese patent of CN101386636 has been introduced a kind of Momordica-Glycosides: comprise that Grosvenor Momordica fragmentation-extraction-extracting solution is with absorption with macroporous adsorbent resin-wash-out resin-collection elutriant-concentrate, dry; Wherein, said macroporous adsorbent resin is the ADS-21 model, or the ADS-17 model, or the ADS-7 model, or the strong polar macroporous adsorption resin of ADS-F8 model.
Publication number is that the Chinese patent of CN101440118 has been introduced a kind of saponins compound that from Momordica grosvenori root, extracts, Its Preparation Method And Use: saponins compound Siraitic acid IIB that extraction separation obtains from Momordica grosvenori root and Siraitic acid IIC, its preparation method and the application in antitumor drug thereof.The molecular formula of above-mentioned two kinds of compounds is respectively C
40H
60O
14And C
40H
60O
15, through the various modern spectroscopic analysis, particularly use the integration analysis of two dimensional NMR spectrum, confirmed the chemical structure of these two kinds of compounds.
Publication number is preparation method and the application thereof that the Chinese patent of CN101444333 has been introduced a kind of Fructus Monordicae extract: 1. dried fructus momordicae was pulverized the 40-100 mesh sieve; The use mixed solvent extracts, and concentrates; Liquid concentrator carries out the short-path distillation first time, obtains light constituent 1# and heavy constituent 2#; Heavy constituent 2# carries out the short-path distillation second time, obtains light constituent 3# and heavy constituent 4#, and 4# is a target components; Take by weighing the 4# component, use 75% dissolve with ethanol, evenly spray is added in the blank pipe tobacco, processes cigarette and smokes panel test; 2. extract, ethanol, Ucar 35, water are to form mixed solvent by weight 2: 3: 5, and consumption is 6～20 times of dried fructus momordicae powder, and extraction time is 1～5h, extracts 2～4 times; 3. concentrate, temperature is 90 ℃, and vacuum tightness is 0.098MPa; 4. short-path distillation, temperature is 50 ℃, vacuum tightness is 3000Pa; 5. short-path distillation, temperature is 80 ℃, vacuum tightness is 46Pa; 6. addition is 0.01%.
Publication number is that the Chinese patent of CN101690573A has been introduced a kind of momordica grosvenori glycoside V content greater than 60% Fructus Monordicae extract working method: with new fresh Fructus Momordicae process: fragmentation → saccharification → water extraction → concentrate → sedimentation is centrifugal → ion exchange resin is refining → macroporous resin is refining → concentrate → aluminum oxide is refining → concentrate → and the technical process of spraying drying → finished product.
Publication number is that the Chinese patent of CN101851265A has been introduced a kind of method of from dried fructus momordicae, extracting the various active composition: with the dried fructus momordicae stoning, again with pericarp and pulp add water dodge carry, centrifugal must throw out and supernatant.Supernatant is twice XAD chromatography of warp respectively, or go up polymeric amide-XAD chromatography, and the decolouring of D213 post gets sweet glycosides, polyphenol; Above-mentioned throw out extracts through acidic solution, the D113 post separate vegeto-alkali; Waste liquid concentrates, and ethanol precipitation gets polysaccharide, oligose; Waste residue gets polysaccharide, oligose, amino acid with the gastric enzyme hydrolysis.
Publication number is that the Chinese patent of CN101863946A has been introduced a kind of method of from dried fructus momordicae, extracting the high-content Momordica grosvenori mogroside V: extraction process comprises the steps: to extract, concentrates, filtration, absorption, decolouring, concentrate drying must contain the Momordica grosvenori mogroside V product.
Publication number be the Chinese patent of CN1375499 introduced a kind of from Grosvenor Momordica the method for extraction separation multiple components: handle broken Grosvenor Momordica with buck again after the water and obtain extracting solution; Handle through Fatty Alcohol(C12-C14 and C12-C18) again and obtain throw out (I) and lysate (II), throw out (I) through the resin anion(R.A) post the more pure processing purified of combining fat obtain momordica grosvenori polysaccharide.Lysate (II) is through polyamide resin column, and the effluent from post obtains Momordica-Glycosides through macroporous resin column combining fat alcohol processing purified again.By the composition of polyamide resin column absorption, concentrate drying promptly obtains grosvenor momordica flavonoid after alkaline fat alcohol washes out.
Publication number is that the Chinese patent of CN1907091 has been introduced a kind of method of removing bitter taste of momordica grosvenori extract: comprising: the Fructus Monordicae extract bullion that 1. will contain 60% to 99 % Momordia grosvenori aglycone adds pure water, and the dissolving mixing obtains Fructus Monordicae extract solution; 2. this solution is passed through anionite-exchange resin and Zeo-karb successively; Or mixed type ion exchange resin; Or pass through anion-exchange membrane and cationic exchange membrane successively to remove impurity; Because most impurity is attracted on ion exchange resin or the ion-exchange membrane, the elutriant that contains Momordia grosvenori aglycone of resulting light color has been sloughed whole bitter substances; 3. obtain the powder of Fructus Monordicae extract at last through spraying drying.
Publication number is that the Chinese patent of CN101147751 has been introduced a kind of method of extracting biologically active components from Momordica grosvenori plant: leaf, rattan, root, stem and the fruit of the Momordica grosvenori plant of doing carried out mechanical disintegration obtain meal; Or the fragment that the crushing of fresh Momordica grosvenori plant is obtained wetting; Soak above-mentioned meal and fragment with clear water or aqueous ethanolic solution or methanol aqueous solution; And heat and extract, obtain extract; Then will extract mixture and filter, filtrating is at room temperature separated with chromatography method through macroporous adsorbent resin, and macroporous adsorptive resins is collected elutriant respectively through wash-out, obtains required active part; Maybe will filtrate and separate, and collect through chloroform/ethanol/water gradient elution and obtain active ingredient through silica gel column chromatography; Last isolating active component, drying obtains active substance.
Publication number is that the Chinese patent of CN101177444 has been introduced a kind of method of from Grosvenor Momordica, extracting Momordica-Glycosides: with Grosvenor Momordica fresh fruit or dry fruit is raw material; With water is to extract medium; Accomplish through multi-stage countercurrent extraction, enzymolysis, membrane sepn and column chromatographic isolation and purification technology; Solved removal of impurities, the separation problem of Momordica-Glycosides in separating, have can be continuously produced, time-saving energy-saving, the macroporous resin life-span is long, product purity is high characteristics.According to the inventive method, be raw material with the fresh fruit, to extract yield and can reach more than 0.8%, the yield of dry fruit can reach more than 1.8%, product purity 60-90%.
Publication number is the Chinese patent of CN101200753 Fructus Monordicae extract of having introduced a kind of momordica grosvenori glycoside V content >=40% and preparation method thereof: with the fresh Fructus Momordicae is raw material, through water extraction → concentrate → enzymolysis → macroporous adsorbent resin refining → ion-exchange resin decolorization → concentrate → spraying drying makes.
Publication number is the preparation method that the Chinese patent of CN101007042 has been introduced the Fructus Monordicae extract of a kind of decolouring, debitterize: use water as solvent, adopt the refluxing extraction method, with extracting liquid filtering, concentrated; Use ethanol elution with decolorizing resin decolouring back, take off flavor, reclaim behind the ethanol again through concentrated, spraying drying with deodorization agent; Pulverize; Decoloured, the Fructus Monordicae extract of debitterize, this extract is white, crystalloid powder, sweet taste is pure; No traditional Chinese medicines flavor, back bitter taste, wherein the Momordica-Glycosides weight content is more than 90% or the Momordica grosvenori mogroside V weight content is more than 60%.
Publication number is that the Chinese patent of CN101029071 has been introduced a kind of method that from Grosvenor Momordica, prepares high-purity Momordia grosvenori aglycone V: step is: (1) is broken into meal with mangosteen powder earlier, adds alcohol reflux, filters, and gets pure medicinal extract; (2) pure medicinal extract is suspended in water,, reclaims the ETHYLE ACETATE mother liquor with ethyl acetate extraction 1 time-3 times; (3) continue with 1 time-3 times mother liquors of n-butanol extraction, reclaim propyl carbinol and get propyl carbinol medicinal extract; (4) with propyl carbinol medicinal extract through polyamide column, water lotion concentrate thick saponin(e; (5) thick saponin(e carries out silica gel column chromatography, chloroform-methanol-water wash-out, collect Momordia grosvenori aglycone v bullion; (6) get Momordia grosvenori aglycone v bullion and carry out chromatography, washing, ethanol elution is collected purity greater than the momordica grosvenori glycoside V more than 95%.
Publication number is the purification preparation method that the Chinese patent of CN101033244 has been introduced a kind of momordica grosvenori alcohol: comprise step: pulverize Grosvenor Momordica earlier (1), adds extraction using alcohol and get pure medicinal extract; (2) pure medicinal extract is suspended in water, and ethyl acetate extraction reclaims ETHYLE ACETATE, gets ETHYLE ACETATE medicinal extract, gets mother liquor; (3) add hydrochloric acid soln and ETHYLE ACETATE in the mother liquor, acid hydrolysis is carried out in heating; Obtain ethyl acetate layer after the hydrolysis, be adjusted to neutrality with alkali lye, separating ethyl acetate, decompression and solvent recovery obtains containing the medicinal extract of momordica grosvenori alcohol; (4) medicinal extract is carried out silica gel column chromatography, the chloroform wash-out reclaims chloroform; Use the chloroform-methanol wash-out again, collect elutriant, reclaim solvent, get the momordica grosvenori alcohol bullion; (5) get the momordica grosvenori alcohol bullion and carry out recrystallization, promptly get purity greater than the momordica grosvenori alcohol more than 95%.
Publication number is the extracting and preparing technique that the Chinese patent of CN101050230 has been introduced a kind of Fructus Monordicae extract: a kind ofly under 40 ℃ and following temperature condition, extract and utilize multistage secondary filter and polyvalent alcohol extraction phase bonded method to prepare nutritive ingredient such as rich vitamin C simultaneously to use supplementary material extract and the preparation technology of mogroside for main sweeting agent extract for main food, wherein extract at low temperature and multistage secondary filter and polyvalent alcohol extraction phase combine to relate to gordian technique of the present invention.
Publication number is the application method that the Chinese patent of CN101244112 has been introduced a kind of blood-sugar decreasing active that from Momordica grosvenori plant, extracts: the blood-sugar decreasing active that will from Momordica grosvenori plant, extract is prepared into medicines hypoglycemic, the treatment mellitus; Or be prepared into the food that hyperglycemia, diabetics is had health-care effect, make the content of blood-sugar decreasing active in said product be not less than 0.001%.The blood-sugar decreasing active that is extracted is meant the summation of the bioactive ingredients that from each position of whole plant of Grosvenor Momordica, extracts, or the bioactive ingredients that from the single position of plant, extracts.The prescription of medicine or protective foods has blood-sugar decreasing active, additive and is used for the carrier of this product form, and is prepared from according to the conventional working method of these products.
Publication number is that the Chinese patent of CN101168556 has been introduced a kind of purifying Momordica grosvenori glycosides and extracted the method for biologically active substance: adopt solvent-extraction process purifying Momordica grosvenori glycosides, extract " Grosvenor Momordica is plain " simultaneously, comprise system liquid, with the moderate non-polar solvent extract, with steps such as the organic solvent extraction of middle polarity, spraying dryings.
Publication number is that the Chinese patent of CN1582701 has been introduced a kind of arhat fructose low in calories and method of manufacture thereof: choosing really,---water is carried, and---------wash-out---concentrated------mix---inclusion recrystallization---drying, get finished product by drying in absorption in filtration in fragmentation.Because invention arhat fructose low in calories; Be that Grosvenor Momordica is carried through water, separate remove insoluble wait impurity after, again through wash-out, concentrate, operation such as drying; Obtain the higher relatively Fructus Monordicae extract of a kind of Momordica-Glycosides content, and then form with plant multi-sugar alcohol inclusion recrystallization.
Publication number is that the Chinese patent of CN102464685A has been introduced a kind of method of from Leaf of Grosvenor Momordica, extracting the flavonoid glycoside composition and products thereof: get Leaf of Grosvenor Momordica, add water boil and extract 2～3 times, each 50～100min filters; Merging filtrate advances macroporous resin adsorption, and washed resin to effluent is colourless; With ethanol or anhydrous methanol wash-out, collect elutriant and remove ethanol or methyl alcohol again, in refrigerator, place 20～30h; Separate out deposition, deposition is used anhydrous alcohol solution, filters; The filtrating evaporate to dryness promptly gets kaempferol-3,7-a-L-two rhamnoside crude extracts.
Publication number is the process for extracting that the Chinese patent of CN102408463A has been introduced a kind of Momordica-Glycosides: utilize the strong and weak different of other composition and Momordica-Glycosides ability in the HPD-100 type macroporous resin adsorption Grosvenor Momordica extracting solution; With appearance on the specific speed; After washing flushing with Different concentrations of alcohol solution, with different elution speeds; Remain soup after merging all wash-outs, the spray-dried Momordica-Glycosides finished product that makes.Wherein Grosvenor Momordica general glycoside weight content is up to more than 95%, and the Momordica grosvenori mogroside V weight content reaches more than 65%.
Publication number is the extraction and purification process that the Chinese patent of CN102138958A has been introduced a kind of Momordica grosvenori plant flavones ingredient: adopt ethanol as extraction solvent, extracting solution passes through purification with macroreticular resin.It is characterized in that may further comprise the steps: drying, chopping, alcohol reflux, filtration and concentrate, post absorption, water elution, ethanol elution, concentrate and dry, dissolve with ethanol and filtration after concentrate drying.
Publication number is preparation method and the application thereof that the Chinese patent of CN102321503A has been introduced a kind of Grosvenor Momordica enriched material: raw materials pretreatment; Twice sherwood oil reflux extraction: Grosvenor Momordica and sherwood oil input are had in the extractor of whipping appts, carry out twice sherwood oil reflux extraction; Oven dry: collect the Grosvenor Momordica after twice extraction of sherwood oil in the extractor, and Grosvenor Momordica is dried; Twice microwave reflux extraction: will throw in the microwave extracter that has whipping appts through the Grosvenor Momordica after oven dry is handled, and add deionized water, and carry out twice microwave reflux extraction; Filter: after first, second time microwave reflux extraction liquid is mixed, filter, remove deposition; Concentrate: incite somebody to action the mixed solution concentrating under reduced pressure of twice microwave reflux extraction liquid after filtering, obtain product Grosvenor Momordica enriched material.
Publication number is that the Chinese patent of CN101085991A has been introduced a kind of method of utilizing yam to produce alcohol: disclose a kind of method of utilizing yam to produce alcohol, it may further comprise the steps: (1) raw potatoes is handled; (2) synchronous liquefying-saccharifying: add the wheat bran of being processed by the mixed of quality proportioning 1: 1.5-2.5 by aspergillus oryzae and head mold, add-on is the 0.02-0.10g/g raw material, and the steaming saccharification temperature is 60-85 ℃ synchronously, time 30-100min; (3) fermentation: per 100 milliliters of beers restrain with fresh yeast 0.04-0.12 with active dry yeast or wine with wine, ammonium sulfate 0.06-0.14 gram, and sal epsom 0.001-0.15 gram is at temperature 28-42 ℃ of bottom fermentation 48-60hr; (4) after the fermentation ends, distill by ordinary method, alcohol gets product.
Adopt above-mentioned technology, have following defective: the one, energy expenditure is big, and alcohol mean consumption mark coal per ton is the 0.8-1.0 ton; The 2nd, starch utilization ratio is lower, generally about 80-84%; The 3rd, be not suitable for utilizing Grosvenor Momordica piece root to produce alcohol; Its reason is to contain some antibacterial substances in the Grosvenor Momordica piece root, and wherein starch is different from staple crop starch, and water-absorbing-retaining power is big during its gelatinization; Paste viscosity is big; Water addition ratio is 1:1.8-2.5, certainly will influence the gelatinization of Grosvenor Momordica starch, and then influences subsequent handling.
Summary of the invention
The technical scheme that the present invention adopts comprises: with Grosvenor Momordica piece root clean, section, rubbing, petroleum ether extraction, saccharification filtration, biological fermentation, distill the ethanol finished product.
Therefore, the present invention provides a kind of starch conversion alcoholic acid method in the Grosvenor Momordica piece root of utilizing, and step comprises:
(1) Grosvenor Momordica piece root is washed, cut into slices, rub with pulverizer;
(2) Grosvenor Momordica piece root and sherwood oil are mixed by a certain percentage, static placement is more than 3 hours;
(3) remove upper strata sherwood oil part, add aspergillus oryzae and root arrhizus fermentation liquid, add complex cellulase simultaneously and carry out starch degradation, get starch and cellulosic hydrolysate;
(4) in its hydrolyzed solution, add certain active dry yeast, add 0.04% potassium hydrogenphosphate and 0.05% ammonium chloride again and ferment;
(5) after the fermentation ends, fermented liquid is distilled according to ordinary method, get the ethanol finished product.
In one embodiment, used aspergillus oryzae, head mold and active dry yeast all is known mikrobe commonly used in step (3), (4), can reach above-mentioned effect with quasi-microorganism.
In another embodiment, add the volume of sherwood oil in the step 2, its volume (L)/Grosvenor Momordica piece root (kg) ratio=2-5:1.
In another embodiment, add the volume of aspergillus oryzae fermented liquid in the step 3, its volume (L)/Grosvenor Momordica piece root (kg) ratio=5-30:100; The volume that adds root arrhizus fermentation liquid, its volume (L)/Grosvenor Momordica piece root (kg) ratio=10-40:100.
In another embodiment, add the weight of active dry yeast in the step 4, its weight (kg)/Grosvenor Momordica piece root (kg) ratio=5-25:100.
The also available known method of said aspergillus oryzae, head mold: bent from test tube strains to the triangular flask kind, the method to ferment tank liquid prepares voluntarily again.Enzyme activity should reach the 12U/mL fermented liquid in the aspergillus oryzae of preparation, the head mold voluntarily.Active dry yeast and complex cellulase can directly be bought from market, and wherein the active dry yeast number 10
9More than/the g, the complex cellulase enzyme activity should reach the 5000U/g dry weight.
Technique effect:
1, used raw material, equipment is common common raw material, equipment in the inventive method, has avoided in the commercial process having reduced production cost widely for the dependence of expensive raw materials, instrument;
2, improve the utilization ratio of starch in the Grosvenor Momordica piece root greatly, practiced thrift starch resource.
3, the inventive method is simple to operate, greatly reduces the alcoholic acid production cost, has simplified production process.
4, the used reagent of the present invention is chemical reagent nontoxic, cheap, volume production, and sophisticated reagent capable of using reclaims routine techniques in the whole process, and this has greatly reduced to the environmental emission waste.
Embodiment
Below, the present invention will further explain with embodiment, but it is not limited to any or the similar instance of these embodiment.
Embodiment 1
Fresh Fructus Momordicae piece root is got 200kg after cleaning impurity elimination, and section is with after rubbing, and the sherwood oil that adds 700L mixes static placement 4 hours; Remove upper strata sherwood oil part, add 20L aspergillus oryzae and 30L root arrhizus fermentation liquid and under 30 ℃, carry out starch degradation, add the 4kg complex cellulase simultaneously and carry out cellulose degradation, get starch and cellulosic hydrolysate; Potassium hydrogenphosphate and 0.05% the ammonium chloride that in its hydrolyzed solution, adds 20kg active dry yeast and 0.04% carries out 30 ℃ of bottom fermentations 60 hours, the fermented liquid alcoholic strength 15% that obtains, and starch and cellulose utilization rate reach 83%; Fermented liquid is distilled according to ordinary method, get the ethanol finished product.
Embodiment 2
Fresh Fructus Momordicae piece root is got 400kg after cleaning impurity elimination, and section is with after rubbing, and the sherwood oil that adds 1200L mixes static placement 4 hours; Remove upper strata sherwood oil part, add 40L aspergillus oryzae and 50L root arrhizus fermentation liquid and under 30 ℃, carry out starch degradation, add the 5kg complex cellulase simultaneously and carry out cellulose degradation, get starch and cellulosic hydrolysate; Potassium hydrogenphosphate and 0.05% the ammonium chloride that in its hydrolyzed solution, adds 30kg active dry yeast and 0.04% carries out 30 ℃ of bottom fermentations 60 hours, the fermented liquid alcoholic strength 19% that obtains, and starch and cellulose utilization rate reach 85%; Fermented liquid is distilled according to ordinary method, get the ethanol finished product.
Embodiment 3
Fresh Fructus Momordicae piece root is got 600kg after cleaning impurity elimination, and section is with after rubbing, and the sherwood oil that adds 2500L mixes static placement 4 hours; Remove upper strata sherwood oil part, add 100L aspergillus oryzae and 150L root arrhizus fermentation liquid and under 30 ℃, carry out starch degradation, add the 5kg complex cellulase simultaneously and carry out cellulose degradation, get starch and cellulosic hydrolysate; Potassium hydrogenphosphate and 0.05% the ammonium chloride that in its hydrolyzed solution, adds 60kg active dry yeast and 0.04% carries out 30 ℃ of bottom fermentations 60 hours, the fermented liquid alcoholic strength 25% that obtains, and starch and cellulose utilization rate reach 89%; Fermented liquid is distilled according to ordinary method, get the ethanol finished product.
Claims (4)
1. the present invention provides a kind of starch conversion alcoholic acid method in the Grosvenor Momordica piece root of utilizing, and step comprises:
(1) Grosvenor Momordica piece root is washed, cut into slices, rub with pulverizer;
(2) Grosvenor Momordica piece root and sherwood oil are mixed by a certain percentage, static placement is more than 3 hours;
(3) remove upper strata sherwood oil part, add aspergillus oryzae and root arrhizus fermentation liquid, add complex cellulase simultaneously and carry out starch degradation, get starch and cellulosic hydrolysate;
(4) in its hydrolyzed solution, add certain active dry yeast, add 0.04% potassium hydrogenphosphate and 0.05% ammonium chloride again and ferment;
(5) after the fermentation ends, fermented liquid is distilled according to ordinary method, get the ethanol finished product.
2. according to the process of claim 1 wherein the volume that adds sherwood oil in the step 2, its volume (L)/Grosvenor Momordica piece root (kg) ratio=2-5:1.
3. according to the method for claim 2, wherein add the volume of aspergillus oryzae fermented liquid in the step 3, its volume (L)/Grosvenor Momordica piece root (kg) ratio=5-30:100; The volume that adds root arrhizus fermentation liquid, its volume (L)/Grosvenor Momordica piece root (kg) ratio=10-40:100.
4. according to the method for claim 3, wherein add the weight of active dry yeast in the step 4, its weight (kg)/Grosvenor Momordica piece root (kg) ratio=5-25:100.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210223516.3A CN102719485B (en) | 2012-07-02 | 2012-07-02 | A kind of method of Starch Conversion ethanol in root tuber using Momordica grosvenori |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210223516.3A CN102719485B (en) | 2012-07-02 | 2012-07-02 | A kind of method of Starch Conversion ethanol in root tuber using Momordica grosvenori |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102719485A true CN102719485A (en) | 2012-10-10 |
| CN102719485B CN102719485B (en) | 2018-03-23 |
Family
ID=46945352
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210223516.3A Expired - Fee Related CN102719485B (en) | 2012-07-02 | 2012-07-02 | A kind of method of Starch Conversion ethanol in root tuber using Momordica grosvenori |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102719485B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102732570A (en) * | 2012-07-02 | 2012-10-17 | 董爱文 | Method for converting ethanol by utilization of carbohydrate substance in grosvenor momordica fruit root tuber |
| CN110041439A (en) * | 2019-03-29 | 2019-07-23 | 湖南华诚生物资源股份有限公司 | A method of extracting starch from Siraitia grosvenorii root tuber |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101041849A (en) * | 2007-04-26 | 2007-09-26 | 湖南南方马铃薯产业化工程技术有限公司 | Method for producing fuel ethanol by using potato as raw material |
| CN101085991A (en) * | 2007-06-28 | 2007-12-12 | 湖南农业大学 | Process for producing alcohol using potato |
| CN101245355A (en) * | 2008-03-28 | 2008-08-20 | 中国科学院过程工程研究所 | Method for producing fuel ethyl alcohol with sweet potato solid state synchronous diastatic fermentation |
-
2012
- 2012-07-02 CN CN201210223516.3A patent/CN102719485B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101041849A (en) * | 2007-04-26 | 2007-09-26 | 湖南南方马铃薯产业化工程技术有限公司 | Method for producing fuel ethanol by using potato as raw material |
| CN101085991A (en) * | 2007-06-28 | 2007-12-12 | 湖南农业大学 | Process for producing alcohol using potato |
| CN101245355A (en) * | 2008-03-28 | 2008-08-20 | 中国科学院过程工程研究所 | Method for producing fuel ethyl alcohol with sweet potato solid state synchronous diastatic fermentation |
Non-Patent Citations (4)
| Title |
|---|
| 刘明 等: "纤维素酶在酒精工业中的应用进展", 《酿酒科技》 * |
| 庞宗文 等: "根霉和米曲霉的淀粉酶系对生木薯粉酒精发酵的影响", 《食品工业科技》 * |
| 汤桂梅: "罗汉果块根有效成份的分离研究", 《中国优秀博硕士学位论文全文数据库 (硕士) 工程科技Ⅰ辑 B014-43》 * |
| 陈全斌 等: "罗汉果块根淀粉的提取及其性质研究", 《食品科学》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102732570A (en) * | 2012-07-02 | 2012-10-17 | 董爱文 | Method for converting ethanol by utilization of carbohydrate substance in grosvenor momordica fruit root tuber |
| CN110041439A (en) * | 2019-03-29 | 2019-07-23 | 湖南华诚生物资源股份有限公司 | A method of extracting starch from Siraitia grosvenorii root tuber |
| CN110041439B (en) * | 2019-03-29 | 2021-06-29 | 湖南华诚生物资源股份有限公司 | Method for extracting starch from momordica grosvenori root tuber |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102719485B (en) | 2018-03-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106008645B (en) | A kind of method that momordica grosvenori glycoside V is extracted from Momordica grosvenori | |
| CN105272988B (en) | A kind of pool extracting method of mulberry leaf active ingredient | |
| CN101497904B (en) | Method for producing xylitol and arabinose at the same time | |
| CN102948758B (en) | Method for extracting buckwheat flavone from buckwheat bran | |
| CN102675398B (en) | A kind of method extracting momordica grosvenori glycoside V and farnesol from Grosvenor Momordica | |
| CN100457765C (en) | Method for producing stabhyose, and method for producing stabhyose and catalpol by using rehmannia root | |
| CN109674843B (en) | Extraction and purification method for comprehensively utilizing dried momordica grosvenori | |
| CN101386636B (en) | Mogroside extraction method from grosvenor momordica | |
| CN102408463A (en) | Extraction method for mogroside | |
| CN113854533B (en) | A method for preparing mogroside, mogrolic acid, fructus Siraitiae Grosvenorii concentrated juice and fructus Siraitiae Grosvenorii fructose from fresh fructus Siraitiae Grosvenorii | |
| CN102675399A (en) | Method for extracting mogroside V and mannitol from corsvenor momordica fruit | |
| CN101747196A (en) | Method for preparing chlorogenic acid by using Jerusalem artichoke | |
| CN111072449B (en) | A method for preparing natural ferulic acid from nigre containing oryzanol | |
| CN106589010B (en) | Method that is a kind of while producing L-arabinose and D- galactolipin | |
| CN102040579A (en) | Method for extracting luteolin from peanut roots, stems, leaves and shells | |
| CN111588043B (en) | Preparation method of dietary fiber based on Siraitia grosvenorii waste | |
| CN102199178A (en) | Extraction process of rebaudioside A (RA) | |
| CN101497903B (en) | Method for selectively converting and shunting biological products | |
| CN101856427B (en) | Method for extracting solanine from potato peels | |
| CN104292366A (en) | Method for simultaneously extracting arabinogalactan and dihydroquercetin from dahurian larch | |
| CN110777173B (en) | Method for preparing momordica grosvenori amino acid by using momordica grosvenori centrifugal waste residues | |
| CN110917240B (en) | Continuous method for separating multiple effective components from cyclocarya paliurus | |
| CN102719485B (en) | A kind of method of Starch Conversion ethanol in root tuber using Momordica grosvenori | |
| CN108997359B (en) | Method for extracting chlorophyll from stevioside production waste residues | |
| CN102690359B (en) | A kind of method extracting starch and cucurbitacin from Fructus Momordicae tuber |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB03 | Change of inventor or designer information |
Inventor after: Guo Shiyin Inventor before: Tang Zhonghai |
|
| CB03 | Change of inventor or designer information | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20171116 Address after: 410128 College of food science and technology, Hunan Agricultural University, Changsha, Hunan Applicant after: Hunan Agricultural University Address before: 410128 College of biological science and technology, Hunan Agricultural University, Hunan, Changsha Applicant before: Tang Zhonghai |
|
| TA01 | Transfer of patent application right | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180323 Termination date: 20190702 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |