CN102703554A - Method for preparing oyster polypeptide from oyster shell - Google Patents
Method for preparing oyster polypeptide from oyster shell Download PDFInfo
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- CN102703554A CN102703554A CN2012101868756A CN201210186875A CN102703554A CN 102703554 A CN102703554 A CN 102703554A CN 2012101868756 A CN2012101868756 A CN 2012101868756A CN 201210186875 A CN201210186875 A CN 201210186875A CN 102703554 A CN102703554 A CN 102703554A
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Abstract
The invention discloses a method for preparing oyster polypeptide from an oyster shell, which comprises the following steps of: processing the oyster shell into fine powder of which the fineness is 400-500 meshes; then, adding pure water equivalent to 15-18 times of parts by weight of the fine oyster powder; after the pure water and the fine oyster powder are mixed, processing for 2-3 hours by an emulsion blender; collecting liquid subjected to emulsion processing; adding a proper quantity of alkali liquor to regulate the pH value to 7.0-7.5; heating to 48-52DEG C; adding neutral protease equivalent to 1.5-2.5% of parts by weight of the fine oyster powder; starting to carry out enzymolysis which lasts for eight hours; heating to 80DEG C; keeping for 10min until enzyme is inactivated; filtering and condensing by nanofiltration equipment of which the relative closure molecular weight is 1000Dalton; adjusting the pressure till 3Mpa; collecting concentrated liquor; spraying, drying and screening by a sieve of 80 meshes; and packaging to obtain a finished product. The added value and the competitive power of the product are improved to accelerate the development of the social economy of the region through deep processing of oyster.
Description
Technical field
The present invention relates to the preparation method of a seed oyster polypeptide, particularly a kind of method that from oyster shells, prepares the oyster polypeptide.
Background technology
Crassostrea rivularis has another name called big oyster, is one of Qiezhou, Guangxi four big famous and precious marine products, and meat can be eaten raw, also can be processed into dried oyster, oyster sauce.Big oyster oyster meat is the fishery products with very high nutrition and health care function of protein-high, lower fat, delicious flavour, and oyster meat protein content surpasses 40%, and is nutritious, and delicious flavour have the title of " marine milk ", also can be used as medicine simultaneously.Big oyster meat is rich in taurine, can promote the human brain development.
In the west, oyster is called as " milk in sea ", " sea fruit ", and Oyster was once thought " among the extra large family your person " in ancient times, and this has affirmed fully the nutritive value of oyster.Oyster contains rich in protein, amino acid, also contains abundant VITAMINs such as VA, VB
1, VB
2, VB
3, VB
12, VC, VD
2, VH etc., contain inorganic salt and trace element in the Oyster, human body is had the important physical effect.
Contain a certain amount of protein in the ostreae testa pulverata of processing with oyster shells; But be difficult to by bio-absorbable; The micromolecule polypeptide class faster than the protein of equivalent, can better be digested and assimilated Activated Ca made by oyster shell oyster polypeptide more far away by the free state amino acid of bio-absorbable speed faster than equivalent.
At present, the method that from oyster shells, prepares the oyster polypeptide mainly contains acid hydrolyzation and enzymolysis process, and the acid hydrolyzation reaction is violent; Reaction process and final product are restive, can cause a large amount of amino acid whose destructions, with the direct enzymolysis of oyster shells; Difficulty is big, the time is long, be difficult for filtration, and it is low to obtain the polypeptide rate.
Along with going from strength to strength of oyster culture industry, the oyster shells resource becomes increasingly abundant, and the oyster deep processing is the only way which must be passed that industry enlarges development.
Summary of the invention
The invention provides the working method of from oyster shells, producing the oyster polypeptide that a kind of technology is simple, efficient is high.
The present invention realizes through following technical scheme:
A kind of working method of from oyster shells, producing the oyster polypeptide comprises the steps:
(1) oyster shells after the clean dry being processed into fineness is 400-500 purpose oyster fine powder;
(2) drop into the oyster fine powder, add the pure water that is equivalent to 15-18 times of weight part of oyster fine powder, after the mixing, handled 2-3 hour through emulsifying mixer;
(3) liquid after the collection emulsifying, adding appropriate alkaline liquor adjustment pH value is 7.0-7.5, heats to 48-52 ℃, adds the neutral protease that is equivalent to 1.5-2.5% times of weight part of oyster fine powder, the beginning enzymolysis, enzymolysis time is 8 hours;
(4) be warming up to 80 ℃, keep 10min enzyme deactivation;
(5) use the relative molecular weight that dams to be the nanofiltration equipment filtering and concentrating of 1000Dalton, regulate pressure to 3Mpa, collect liquid concentrator, spraying drying is crossed 80 mesh sieves, pack finished product.
The working method of from oyster shells, producing the oyster polypeptide of the present invention, said oyster shells: moisture content≤5.0%, protein>=50%, ash≤3.0%, fat≤5.0%; Neutral protease enzyme activity 130000u/g; Protein contnt>=60% of the oyster polypeptide products that obtains.
It is raw material that the present invention is processed into 400-500 purpose fine powder with oyster shells, and being the contriver finds through a large amount of comparative experimentss, and the quality of product is not that the granularity with the oyster shells segmentation is directly proportional, and lists the part correlation data at present:
In the product that the present invention obtains, protein contnt is more than 60%, and the molecular weight of 95% oyster polypeptide is below 1000Dalton.Following table obtains oyster polypeptide molecular weight distribution table for the present invention:
The invention has the advantages that:
1, the present invention finds with the granularity to be that 400-500 purpose oyster shells fine powder is a raw material through a large amount of experiments, can realize the quality of product and the perfect balance of production cost;
2, oyster fine powder of the present invention becomes the oyster polypeptide through enzymolysis, and technology is simple, and efficient is high; The oyster polypeptide products that obtains is that white, no fishy smell, mouthfeel are good; Contained mixed polypeptide molecular weight is little, can be directly uses as protective foods, can be used as also that other compositions of protective foods, makeup combine in protective foods, cosmetic material and the prior art; Adopt prior art, prepare various forms of protective foodss, makeup;
3, through utilizing deep processing, improve product value added and competitive power, promoted this area The development in society and economy oyster.
Embodiment
The present invention will be described with embodiment below, but the present invention is not limited to these embodiment.
Embodiment 1
A kind of working method of from oyster shells, producing the oyster polypeptide comprises the steps:
(1) oyster shells after the clean dry being processed into fineness is 400-500 purpose oyster fine powder;
(2) drop into oyster fine powder 10kg, add the pure water of 150kg, after the mixing, handled 3 hours through emulsifying mixer;
(3) liquid after the collection emulsifying, adding appropriate alkaline liquor adjustment pH value is 7.0, heats to 52 ℃, adds the neutral protease of 250g, the beginning enzymolysis, enzymolysis time is 8 hours;
(4) be warming up to 80 ℃, keep 10min enzyme deactivation;
(5) use the relative molecular weight that dams to be the nanofiltration equipment filtering and concentrating of 1000Dalton, regulate pressure to 3Mpa, collect liquid concentrator, spraying drying is crossed 80 mesh sieves, pack finished product.
The working method of from oyster shells, producing the oyster polypeptide of the present invention, said oyster shells: moisture content≤5.0%, protein>=50%, ash≤3.0%, fat≤5.0%; Neutral protease enzyme activity 130000u/g; The protein contnt of the oyster polypeptide products that obtains is 62%.
Embodiment 2
A kind of working method of from oyster shells, producing the oyster polypeptide comprises the steps:
(1) oyster shells after the clean dry being processed into fineness is 400-500 purpose oyster fine powder;
(2) drop into oyster fine powder 10kg, add the pure water of 180kg, after the mixing, handled 2 hours through emulsifying mixer;
(3) liquid after the collection emulsifying, adding appropriate alkaline liquor adjustment pH value is 7.5, heats to 48 ℃, adds the neutral protease of 150g, the beginning enzymolysis, enzymolysis time is 8 hours;
(4) be warming up to 80 ℃, keep 10min enzyme deactivation;
(5) use the relative molecular weight that dams to be the nanofiltration equipment filtering and concentrating of 1000Dalton, regulate pressure to 3Mpa, collect liquid concentrator, spraying drying is crossed 80 mesh sieves, pack finished product.
The working method of from oyster shells, producing the oyster polypeptide of the present invention, said oyster shells: moisture content≤5.0%, protein>=50%, ash≤3.0%, fat≤5.0%; Neutral protease enzyme activity 130000u/g; The protein contnt of the oyster polypeptide products that obtains is 63%.
Claims (1)
1. a method that from oyster shells, prepares the oyster polypeptide comprises the steps:
(1) oyster shells after the clean dry being processed into fineness is 400-500 purpose fine powder;
(2) drop into the oyster fine powder, add the pure water that is equivalent to 15-18 times of weight part of oyster fine powder, after the mixing, handled 2-3 hour through emulsifying mixer;
(3) liquid after the collection emulsifying, adding appropriate alkaline liquor adjustment pH value is 7.0-7.5, heats to 48-52 ℃, adds the neutral protease that is equivalent to 1.5-2.5% times of weight part of oyster fine powder, the beginning enzymolysis, enzymolysis time is 8 hours;
(4) be warming up to 80 ℃, keep 10min enzyme deactivation;
(5) use the relative molecular weight that dams to be the nanofiltration equipment filtering and concentrating of 1000Dalton, regulate pressure to 3Mpa, collect liquid concentrator, spraying drying is crossed 80 mesh sieves, pack finished product.
Said oyster shells: moisture content≤5.0%, protein>=50%, ash≤3.0%, fat≤5.0%; Neutral protease enzyme activity 130000u/g; Protein contnt>=60% of the oyster polypeptide products that obtains.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN 201210186875 CN102703554B (en) | 2012-06-07 | 2012-06-07 | Method for preparing oyster polypeptide from oyster shell |
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|---|---|---|---|
| CN 201210186875 CN102703554B (en) | 2012-06-07 | 2012-06-07 | Method for preparing oyster polypeptide from oyster shell |
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| CN102703554A true CN102703554A (en) | 2012-10-03 |
| CN102703554B CN102703554B (en) | 2013-11-06 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102993268A (en) * | 2012-12-24 | 2013-03-27 | 南京中医药大学 | Preparation method of oyster shell antioxidation peptide |
| CN104673873A (en) * | 2015-03-31 | 2015-06-03 | 广西还珠海洋生物科技有限公司 | Method for extracting antioxidative peptide from oyster shell |
| CN104694606A (en) * | 2015-03-31 | 2015-06-10 | 广西还珠海洋生物科技有限公司 | Method for preparing small molecule peptide by using compound enzyme to hydrolyze oyster protein |
| CN105433300A (en) * | 2015-12-29 | 2016-03-30 | 广西钦州市绿源天然食品加工有限公司 | Processing method for producing active calcium from oyster shells |
| CN106480145A (en) * | 2016-10-14 | 2017-03-08 | 冯群力 | A kind of extracting method of small molecule oyster polypeptide |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1887298A (en) * | 2005-07-03 | 2007-01-03 | 大连玉璘企业集团有限公司 | Prepn process of oyster polysaccharide-mucleic acid-nanometer calcium composition |
| CN101263860A (en) * | 2008-05-05 | 2008-09-17 | 山东天久生物技术有限公司 | Industrial production method for making peptide of oyster from oyster powder by enzyme method |
-
2012
- 2012-06-07 CN CN 201210186875 patent/CN102703554B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1887298A (en) * | 2005-07-03 | 2007-01-03 | 大连玉璘企业集团有限公司 | Prepn process of oyster polysaccharide-mucleic acid-nanometer calcium composition |
| CN101263860A (en) * | 2008-05-05 | 2008-09-17 | 山东天久生物技术有限公司 | Industrial production method for making peptide of oyster from oyster powder by enzyme method |
Non-Patent Citations (1)
| Title |
|---|
| 申淑琦，等: "牡蛎的营养保健功能及其开发利用", 《河北农业科学》, vol. 13, no. 10, 31 December 2009 (2009-12-31), pages 79 - 81 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102993268A (en) * | 2012-12-24 | 2013-03-27 | 南京中医药大学 | Preparation method of oyster shell antioxidation peptide |
| CN104673873A (en) * | 2015-03-31 | 2015-06-03 | 广西还珠海洋生物科技有限公司 | Method for extracting antioxidative peptide from oyster shell |
| CN104694606A (en) * | 2015-03-31 | 2015-06-10 | 广西还珠海洋生物科技有限公司 | Method for preparing small molecule peptide by using compound enzyme to hydrolyze oyster protein |
| CN104694606B (en) * | 2015-03-31 | 2018-04-20 | 广西还珠海洋生物科技有限公司 | A kind of method that small-molecular peptides are prepared with complex enzyme for hydrolyzing Oyster Protein |
| CN105433300A (en) * | 2015-12-29 | 2016-03-30 | 广西钦州市绿源天然食品加工有限公司 | Processing method for producing active calcium from oyster shells |
| CN106480145A (en) * | 2016-10-14 | 2017-03-08 | 冯群力 | A kind of extracting method of small molecule oyster polypeptide |
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| CN102703554B (en) | 2013-11-06 |
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