CN102703465A - Salt-tolerant drought-tolerant wheat gene TaWRKY79 and application thereof - Google Patents
Salt-tolerant drought-tolerant wheat gene TaWRKY79 and application thereof Download PDFInfo
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Abstract
The invention belongs to the technical field genetic engineering and relates to cloning and application of salt-tolerant drought-tolerant WRKY wheat transcription factor gene TaWRKY79. The invention also relates to the salt-tolerant drought-tolerant WRKY wheat transcription factor gene TaWRKY79 and application thereof to breeding salt-tolerant drought-tolerant plants. Experiments show that salt tolerance and drought tolerance of the transgenic plants are improved evidently. The salt-tolerant drought-tolerant WRKY wheat transcription factor gene TaWRKY79 plays an important role on breeding the salt-tolerant drought-tolerant plants (especially crops).
Description
Technical field
The invention belongs to gene engineering technology field, relate to wheat salt tolerance, drought resisting WRKY transcription factor gene
TaWRKY79Clone and application thereof.
Background technology
The soil salinization and shortage of water resources are global problems of restriction agriculture prodn in recent years.Salt damage and arid not only have a strong impact on growth and development of plant, cause crop failure, and make ecotope go from bad to worse (Saibo et al., Annals of Botany, 2009,103 (4): 609-623).Therefore, drought resisting of raising crop and/or salt resistance ability have become one of key issue of modern crop breeding work urgent need solution.The separating clone resistant gene of salt, cultivating the salt tolerant new variety has become one of global research focus.Wheat is important farm crop, clone's salt tolerant, anti-drought gene, and cultivation salt tolerant, drought resisting new variety of wheat have become when the urgent task of previous ten minutes.
The research that utilizes genetic engineering technique to carry out plant salt tolerance, drought resisting aspect has at present obtained bigger progress.Some experiments show, in gene transferred plant relevant in plant itself and the other biological with salt tolerant, saline-alkaline tolerance that can the render transgenic plant improve (Chinnusamy et al., Crop science, 2005,45:437-448.).
Transcription factor plays crucial regulating and controlling effect in genetic expression and environment-stress response.At present, found that some can significantly improve the transcription factor of plant salt tolerance ability.Research shows, these gene transformation in plant, can obviously be improved the salt resistance ability (Nakashima K., Plant Physiology, 2009,149:88 – 95.) of plant.
WRKY is a transcription factor family, and the normal growth of its member's wide participation plant is grown and to the response process of various environment-stress.The transgenic research experiment showed expresses salt tolerant and the drought tolerance that some WRKY transcription factor genes can improve transfer-gen plant.Example is expressed soybean excessively
GmWRKY54But the salt tolerant of gene render transgenic Arabidopis thaliana and drought tolerance raising (Zhou et al., Plant Biotechnology Journal, 2008,6,486-503.).With barley
HvWRKY38Change herbage over to and can improve living weight and the drought tolerance (Xiong et al., Molecular Breeding, 2010,25:419 – 432.) of transgenic herbage.
AtWRKY25, AtWRKY33Cross and express Arabidopis thaliana and also increased tolerance salt stress.The at present relevant research of wheat WRKY transcription factor aspect salt tolerant is also very limited, and Niu et al research showed expression
TaWRKY2With
TaWRKY19Can improve the salt tolerant and the drought tolerance (Niu et al., Plant cell and environment, 2012) of transgenic arabidopsis.Further clone's wheat salt tolerance, non-irrigated genes involved, through genetically engineered cultivate wheat salt tolerance, drought-enduring new variety are extremely important.
Summary of the invention
The objective of the invention is to, a kind of WRKY transcription factor gene of from wheat, isolating a salt tolerant, drought resisting first is provided, called after
TaWRKY79This gene was connected on the expression vector pCAMBIA super 1300, utilizes During Agrobacterium method arabidopsis thaliana transformation, the living weight of the transgenic arabidopsis plant of acquisition, salt tolerance, drought-resistant ability are all than not transgenic adjoining tree raising.
Technical scheme of the present invention is: a kind of wheat salt tolerance, anti-drought gene
TaWRKY79,
TaWRKY79The cDNA sequence of gene is shown in SEQ ID No.1, and aminoacid sequence is shown in SEQ ID No.2.
Wheat salt tolerance of the present invention, anti-drought gene
TaWRKY79The preparation method: at first select the WRKY transcription factor gene (probe) that in the wheat seedling root, receives the remarkable up-regulated expression of salt stress according to the gene chip expression spectral-data of wheat; Then according to probe sequence design gene-specific primer; Further its full-length cDNA of clone from the full-length cDNA library of wheat root made up expression vector at last and is transformed into and carries out functional study in the Arabidopis thaliana.
1. design of primers
Downstream primer Wrky1-1:5 '-GCTGCATCCACTCTAGAATGTCG-3 ' according to chip probe sequences Design gene specific; Matching with 5 of library ' end anchor primer NT3:5 '-ACTAAAGggaACAAAAGCTGG AG-3 ', is the full-length cDNA of template amplification gene with the full-length cDNA library of wheat seedling root.
2.PCR reaction system (50 μ L) and program
2×GC?bufferⅠ 25μl
Template cDNA library 1ul
dNTPs（10mM?each） 1μl
Primer1?(10μM) 1μl
Primer2(10μM) 1μl
LA?Taq(TaKaRa) 0.5ul
DdH
2O adds to final volume 50 μ l
94 ℃ of preparatory sex change 3min; 94 ℃ of sex change 45sec, 58 ℃ of renaturation 1min, 72 ℃ are extended 2min, circulate 35 times; 72 ℃ are extended 5min.
3.1% agarose gel electrophoresis
Pcr amplification product detects the band (Fig. 1) that clauses and subclauses are found to have at the 1000bp place in the back with 1% agarose gel electrophoresis.
4. the recovery of amplified fragments, with the linking of T carrier
Adopt the agarose gel of Tiangen company to reclaim test kit to amplified band, the step by specification carries out.The PCR product is connected with pGEM-T (Promega) carrier, and linked system is:
The PCR product 7 μ l that reclaim
10 * T4 ligase enzyme damping fluid, 1 μ l
PGEM-T carrier (50ng/ μ l) 1 μ l
T4DNA ligase enzyme (3U/ μ l) is 1 μ l (Takara)
Spend the night in 4 ℃ of refrigerators connections.
5. reclaim segmental clone and order-checking
Use CaCl
2Legal system is equipped with escherichia coli DH5a (is epoch biotech firms available from the sky) competent cell, and 42 ℃ of water-bath heat shock methods transform.Cultivate and add X-gal and IPTG in the bacterium liquid of 1h to transforming the back, mixing evenly is coated onto it on flat board that contains penbritin with spreader, and 37 ℃ of constant temperature are inverted overnight cultures, when waiting to occur obvious single bacterium colony flat board is taken out.
According to the T7 promotor and the SP6 promoter sequence design universal primer T7 at two ends, used pGEM-T carrier cloning site, SP6 carries out PCR to recombinant clone to be identified.
PCR program: 94 ℃ of preparatory sex change 10min; 94 ℃ of sex change 1min, 58 ℃ of renaturation 1min, 72 ℃ are extended 1min, circulate 35 times; 72 ℃ are extended 5min;
Pcr amplification product detects with 1% agarose electrophoresis, and the bacterium liquid of getting positive colony send company to check order.Sequencing result is seen SEQ ID No.1.
The present invention also provides wheat salt tolerance, anti-drought gene
TaWRKY79Application in cultivating salt tolerant, drought-resistant plant.Especially the application in common wheat, corn and rice plants.
The invention has the beneficial effects as follows: the present invention clones first and has obtained wheat WRKY transcription factor gene
TaWRKY79, this gene was connected on the expression vector pCAMBIA super 1300, utilize During Agrobacterium method arabidopsis thaliana transformation, the living weight of the transgenic arabidopsis plant of acquisition, salt tolerance, drought-resistant ability all obviously improve than transgenic adjoining tree not.
Description of drawings
Fig. 1
TaWRKY79The amplification of full length gene cDNA sequence.M:DNA molecular weight marker.
Under Fig. 2 different treatment condition
TaWRKY79Gene melts the RT-PCR expression analysis in No. 3 seedling roots and the leaf on the wheat mountain.
TaActinBe confidential reference items; Leaf: leaf; Root: root.
The enzyme of the plant expression vector pCAMBIA-super1300/TaWRKY79 that Fig. 3 makes up is cut the checking result.
The PCR of Fig. 4 transgenic arabidopsis identifies.1,2,6: different transgenic arabidopsis strain systems, Col-0 wild-type Arabidopis thaliana.
The phenotypic evaluation of Fig. 5 transgenic arabidopsis strain system.
A, B: the phenotype of contrast and transgenic line under the normal growth condition.
C: the phenotype of plant under the different treatment condition.
D-H: the long statistics of contrast and transgenic line plant root under the different treatment condition.
I: the wild-type Arabidopis thaliana with
TaWRKY79Transgenic arabidopsis not homophyletic is arid 2 weeks, the phenotype of rehydration after 3 days.
Embodiment
1. design of primers
Downstream primer Wrky1-1:5 '-GCTGCATCCACTCTAGAATGTCG-3 ' according to chip probe sequences Design gene specific; Matching with 5 of library ' end anchor primer NT3:5 '-ACTAAAGggaACAAAAGCTGG AG-3 ', is the full-length cDNA of template amplification gene with the full-length cDNA library of wheat seedling root.
2.PCR reaction system (50 μ L) and program
2×GC?bufferⅠ 25μl
Template cDNA library 1ul
dNTPs（10mM?each） 1μl
Primer1?(10μM) 1μl
Primer2(10μM) 1μl
LA?Taq(TaKaRa) 0.5ul
DdH
2O adds to final volume 50 μ l
94 ℃ of preparatory sex change 3min; 94 ℃ of sex change 45sec, 58 ℃ of renaturation 1min, 72 ℃ are extended 2min, circulate 35 times; 72 ℃ are extended 5min.
3.1% agarose gel electrophoresis
Pcr amplification product detects the band (Fig. 1) that clauses and subclauses are found to have at the 1000bp place in the back with 1% agarose gel electrophoresis.
4. the recovery of amplified fragments, with the linking of T carrier
Adopt the agarose gel of Tiangen company to reclaim test kit to amplified band, the step by specification carries out.The PCR product is connected with pGEM-T (Promega) carrier, and linked system is:
The PCR product 7 μ l that reclaim
10 * T4 ligase enzyme damping fluid, 1 μ l
PGEM-T carrier (50ng/ μ l) 1 μ l
T4DNA ligase enzyme (3U/ μ l) is 1 μ l (Takara)
Spend the night in 4 ℃ of refrigerators connections.
5. reclaim segmental clone and order-checking
Use CaCl
2Legal system is equipped with escherichia coli DH5a (is epoch biotech firms available from the sky) competent cell, and 42 ℃ of water-bath heat shock methods transform.Cultivate and add X-gal and IPTG in the bacterium liquid of 1h to transforming the back, mixing evenly is coated onto it on flat board that contains penbritin with spreader, and 37 ℃ of constant temperature are inverted overnight cultures, when waiting to occur obvious single bacterium colony flat board is taken out.
According to the T7 promotor and the SP6 promoter sequence design universal primer T7 at two ends, used pGEM-T carrier cloning site, SP6 carries out PCR to recombinant clone to be identified.
PCR program: 94 ℃ of preparatory sex change 10min; 94 ℃ of sex change 1min, 58 ℃ of renaturation 1min, 72 ℃ are extended 1min, circulate 35 times; 72 ℃ are extended 5min;
Pcr amplification product detects with 1% agarose electrophoresis, and the bacterium liquid of getting positive colony send company to check order.Sequencing result is seen SEQ ID No.1.
Under embodiment 2, the different treatment condition
TaWRKY79Expression of gene is analyzed
1. material processing
No. 3 seed room temperature sprouting is melted on the wheat mountain, removes endosperm after 1 week, continues to cultivate with the Hangload liquid nutrient medium and 1 week coerces processing.
Salt stress: in the Hangload liquid nutrient medium, add NaCl to final concentration be 200mM;
Osmotic stress: add in the substratum PEG to final concentration be 18%;
ABA handles: add in the substratum ABA to final concentration be 100 μ M;
4 ℃ of deepfreezes: the wheat seedling of liquid culture is moved to 4 ℃ of illumination boxs cultivations;
Handle blade and the root system of getting seedling after 0,0.5,3,12,24,48 hour respectively under the different condition, extract total RNA of various materials.
2. the total RNA of wheat extracts
The Trizol method is extracted RNA.The RNA quality that the 1%Agrose detected through gel electrophoresis is extracted, the RNA concentration that UV spectrophotometer measuring is extracted.
3. the first chain cDNA's is synthetic
Adopt PrimeScript
TMRT-PCR Kit, reactions step is undertaken by operational manual.
4.RT-PCR reaction and electrophoresis
1. be template with cDNA, carry out the PCR reaction.Primer is following
TaAct-S:?5′-?GTTCCAATCTATGAGGGATACACGC?-3′
TaAct-A:?5′-?GAACCTCCACTGAGAACAACATTACC?-3′
WRKYrt-1:?5′-AAGGCTCTGGCGGCAGGAAG-3′
WRKYrt-2:?5′-GCTGCATCCACTCTAGAATGTCG-3′
2.PCR system
ddH
2O 4.7μl
10×?buffer 2μl
Primer1（2μM） 1μl
Primer2（2μM） 1μl
dNTP（10mM?each） 0.2μl
rTaq?polymerase（5U/μl） 0.1μl
Total?Volume 10μl
3.PCR program
94 ℃ of 5min; According to the gene expression amount difference 25～30 cycles are set, 94 ℃ of 20s, 57 ℃ of 60s, 72 ℃ of 45s; 72 ℃ of 5min.
4.1% agarose gel electrophoresis.The result sees Fig. 2.
Plant expression vector pCAMBIA-super1300 is the binary vector that contains 35S promotor and NPT II gene, on its MCS, contains restriction enzyme
XbaThe I site.According to gene
TaWRKY79The cDNA sequence, gene-specific primer Wrkyo5:5 '-GCTCTAGAATGGACGAGCAGTGGATGATC-3 ' that design comprises complete ORF is (XbaI) and Wrkyo3:5 '-GCTCTAGACGCTCGCTTGATCAACTATCG-3 ' is (XbaI).Use this to primer amplification
TaWRKY79The cDNA sequence.Use restriction enzyme then
XbaThe I enzyme is cut the goal gene of carrier pCAMBIA-super1300 and amplification
TaWRKY79Sequence.The carrier of complete degestion reclaims after 1% agarose gel electrophoresis separates with goal gene; Link; Transformed into escherichia coli DH5 α, PCR detects positive colony, extracts the plasmid of positive colony; Enzyme is cut the exactness of checking direction of insertion, makes up to obtain plant expression vector pCAMBIA-super1300/TaWRKY79.Step is following:
(1) plasmid pCAMBIA-super1300 empty carrier and goal gene segment
XbaThe I single endonuclease digestion
It is following that enzyme is cut system:
The pCAMBIA-super1300 carrier
(or target gene fragment) 10 μ l
10×Buffer?M 2?μl
ddH
2O?To 40?μl
Cut more than 3 hours in 37 ℃ of thermostat water bath enzymes.
(2) enzyme is cut the electrophoresis and the recovery of product
After enzyme cuts, be electrophoretic buffer, enzyme cut product carry out 1% agarose gel electrophoresis with 1 * TAE.Under ultraviolet transilluminator, downcut the big fragment of pCAMBIA-super1300 carrier and the target gene fragment of cutting through enzyme with clean blade, sepharose reclaims test kit and reclaims the purpose band.
(3) connect
The pCAMBIA-super1300 carrier segments of cutting through enzyme and target gene fragment are carried out 4 ℃ with the ratio of mol ratio 1:4 and are connected and spend the night.
(4) transform
Connect product heat shock method transformed into escherichia coli DH5 α competent cell, transformed bacteria on the LB solid plate that contains Kan 50 μ g/ml 37 ℃ cultivated about 16 hours.
(5) evaluation of recon
PCR detects positive colony, extracts plasmid, selects the BamHI restriction enzyme site on intragenic BamHI single endonuclease digestion site and the carrier that plasmid is carried out single endonuclease digestion evaluation target fragment direction of insertion.Enzyme is cut product behind 1% agarose gel electrophoresis, judges that according to the size of the purpose band that downcuts goal gene inserts the direction in the carrier, chooses the plasmid that forward inserts the clone, accomplishes vector construction (Fig. 3).The carrier pCAMBIA-super1300/TaWRKY79 that builds is transformed agrobacterium strains GV3101, and arabidopsis thaliana transformation carries out gene function analysis.
The functional analysis of embodiment 4, gene---Arabidopis thaliana conversion, screening and phenotype analytical
(1) plantation of Arabidopis thaliana
Wild-type Arabidopis thaliana seed with 7.5% chlorine bleach liquor (comprising 7.5% Youxiaolin and 0.01% Triton-X 100) sterilization 15 minutes, is used rinsed with sterile water 5-6 time then, and point was sowed on the MS flat board, in 4 ° of C vernalization 2-3 days.Be transplanted to then (nutrition soil mixes by equal proportion with vermiculite) in the Nursery, 23 ° of C cultivate 16/8 h photoperiod, light intensity 30-40 μ molm-2 s-1; After treating plant blossom, cut off its major branch top, promote the side shoot development.In 4-6 after beta pruning days, carry out Agrobacterium-mediated Transformation.
(2) Arabidopis thaliana transforms
Pour 200 ml bacterium liquid in the tray into.With pruning good Arabidopis thaliana back-off and all inflorescences being immersed in the suspension bacteria liquid, stir to be stained with gently and spend 30 sec-1 min.Take out flowerpot and be sidelong in pallet, wrap up to preserve moisture with freshness protection package.Pallet is put the dark place cultivate 24 h.Take out Nursery and upright the placement then, recover illumination, continue to cultivate plant to ripe.
(3) after the screening of positive plant: T0 sterilized with 7.5% chlorine bleach liquor (comprising 7.5% Youxiaolin and 0.01% Triton-X100) for seed, program request was selected on the culture plate (30 mg/L Totomycin) at MS.In 4 ℃ of following vernalization 2-3 days.Move in the culturing room and cultivate.About about 10 days, select hygromycin resistance plant (it is right to grow true leaf 1-2, and root is stretched in the substratum) and be transplanted in the Nursery.Cultivation is until seed maturity.T1 obtains T2 for plant for seed with the quadrat method screening.And select resistance in for plant at T2 and insert independent strain system than the single copy for 3:1, and the T3 that obtains to isozygoty carries out the Molecular Detection and the phenotypic evaluation of transgenic arabidopsis for strain system.
(4) PCR of transgenic arabidopsis identifies
The extraction of arabidopsis thaliana genomic dna
The CTAB method is extracted the genomic dna of wild-type and transgenic arabidopsis strain system.
Amplification
Arabidopsis thaliana genomic dna with top extraction is a template, uses gene-specific primer Wrkyo5, and Wrkyo3 (sequence is with embodiment 3) carries out pcr amplification.
PCR system and program are with embodiment 1.Pcr amplification product amplifies a purpose band about 1000bp through detecting behind the agarose gel electrophoresis in the transgenic arabidopsis plant, do not seeing amplified band (Fig. 4) in the transgenic adjoining tree.
(5) phenotypic evaluation of transgenic arabidopsis
1. the plantation of Arabidopis thaliana
T3 sterilized 15 minutes with 7.5% chlorine bleach liquor (comprising 7.5% Youxiaolin and 0.01% Triton-X 100) for single seed that copies the Arabidopis thaliana strain system of isozygotying; Use rinsed with sterile water 5-6 time then, point was sowed on the MS flat board, in 4 ° of C vernalization 2-3 days; Be transplanted to then (nutrition soil mixes by equal proportion with vermiculite) in the Nursery; 23 ° of C cultivate 16/8 h photoperiod, light intensity 30-40 μ molm-2 s-1.
2. coerce processing
The Arabidopis thaliana seedling (contrast and transgenic line) that sprouted 5 days carefully is transplanted in the nutrition soil; Or transfer to and contain respectively on 100mM NaCl, 100mM N.F,USP MANNITOL, 10mM LiCl, 2 μ M ABA, 0.75 μ M NAA and the contrast MS culture medium flat plate, vertically cultivate a week and observe phenotype.The result shows: under the normal cultured condition
TaWRKY79Transgenic arabidopsis ratio not transgenic contrast has growth potential preferably, and the living weight increase (Fig. 5 A, B).Under the different treatment condition, change
TaWRKY79The root system of the Arabidopis thaliana plant of gene obviously is longer than not transgenic contrast strain system (Fig. 5 C-H).
Arid is handled: the Arabidopis thaliana seedling (contrast and transgenic line) that will sprout a week transfers load in the compost, begins to control the processing of water (not watering) arid after cultivating for 1 week.Arid is handled 2 weeks back observation phenotype.After the result showed that arid handled for 2 weeks, not genetically modified contrast Arabidopis thaliana plant began to wilt, and transforms
TaWRKY79The Arabidopis thaliana plant growing way of foreign gene vigorous (Fig. 5 I).Rehydration is handled after 3 days and is observed phenotype once more, finds to transform
TaWRKY79The Arabidopis thaliana plant of gene grows fine, and bolting is bloomed, and growth obviously is better than not transgenic adjoining tree (Fig. 5 I).
Claims (4)
1. a wheat salt tolerance, anti-drought gene
TaWRKY79, it is characterized in that: the nucleotide sequence of said gene cDNA is shown in SEQ ID No.1.
2. a kind of wheat salt tolerance as claimed in claim 1, anti-drought gene
TaWRKY79, it is characterized in that: said transcription factor gene encoded protein matter, its aminoacid sequence are shown in the SEQ ID No.2.
3. a kind of according to claim 1 wheat salt tolerance, anti-drought gene
TaWRKY79Application in cultivating salt tolerant, drought-resistant plant.
4. a kind of wheat salt tolerance as claimed in claim 3, anti-drought gene
TaWRKY79Application, it is characterized in that: said plant is common wheat, corn and paddy rice.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103667315A (en) * | 2013-12-11 | 2014-03-26 | 济南大学 | Salt-tolerant and drought-resistant gene TaDHN1 of wheat, recombinant plasmid and application |
| CN103695439A (en) * | 2013-12-25 | 2014-04-02 | 华中农业大学 | Fortunella.crassifolia FcWRKY70 gene and application of gene in improving drought tolerance of plants |
| CN103804478A (en) * | 2012-11-14 | 2014-05-21 | 中国农业科学院作物科学研究所 | Stress tolerance associated protein TaSAP1 of plants as well as coding gene and application thereof |
| CN109182353A (en) * | 2018-10-08 | 2019-01-11 | 四川农业大学 | A kind of close Luo Mu gene M fWRKY17 and its application |
| CN110066327A (en) * | 2019-04-30 | 2019-07-30 | 河北省农林科学院遗传生理研究所（河北省农林科学院农产品质量安全研究中心） | Application of the protein TaWRKY13 in regulation stress resistance of plant |
| CN115786361A (en) * | 2022-09-02 | 2023-03-14 | 青岛农业大学 | New application of wheat TaCBF14B gene |
-
2012
- 2012-05-04 CN CN2012101355401A patent/CN102703465A/en active Pending
Non-Patent Citations (2)
| Title |
|---|
| QIN,Y.X. AND XIA,G.M.: "GenBank:AFN44008.1 WRKY79 transcription factor [Triticum aestivum]", 《NCBI GENBANK》 * |
| QIN,Y.X. AND XIA,G.M: "GenBank: JX047374.1 Triticum aestivum WRKY79 transcription factor (WRKY79) mRNA, complete cds", 《NCBI GENBANK》 * |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103804478A (en) * | 2012-11-14 | 2014-05-21 | 中国农业科学院作物科学研究所 | Stress tolerance associated protein TaSAP1 of plants as well as coding gene and application thereof |
| CN103667315A (en) * | 2013-12-11 | 2014-03-26 | 济南大学 | Salt-tolerant and drought-resistant gene TaDHN1 of wheat, recombinant plasmid and application |
| CN103695439A (en) * | 2013-12-25 | 2014-04-02 | 华中农业大学 | Fortunella.crassifolia FcWRKY70 gene and application of gene in improving drought tolerance of plants |
| CN103695439B (en) * | 2013-12-25 | 2015-10-21 | 华中农业大学 | Gold mandarin orange FcWRKY70 gene and the application in raising drought tolerance in plants thereof |
| CN109182353A (en) * | 2018-10-08 | 2019-01-11 | 四川农业大学 | A kind of close Luo Mu gene M fWRKY17 and its application |
| CN109182353B (en) * | 2018-10-08 | 2019-10-22 | 四川农业大学 | A kind of close Luo Mu gene M fWRKY17 and its application |
| CN110066327A (en) * | 2019-04-30 | 2019-07-30 | 河北省农林科学院遗传生理研究所（河北省农林科学院农产品质量安全研究中心） | Application of the protein TaWRKY13 in regulation stress resistance of plant |
| CN115786361A (en) * | 2022-09-02 | 2023-03-14 | 青岛农业大学 | New application of wheat TaCBF14B gene |
| CN115786361B (en) * | 2022-09-02 | 2024-05-10 | 青岛农业大学 | New application of wheat TaCBF B gene |
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Application publication date: 20121003 |