CN102696413B - Method for preventing and controlling tobacco mosaic diseases in field planting period of tobacco floating seedling raising - Google Patents
Method for preventing and controlling tobacco mosaic diseases in field planting period of tobacco floating seedling raising Download PDFInfo
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Abstract
The invention discloses a method for preventing and controlling tobacco mosaic diseases in field planting period of tobacco floating seedling raising. The method comprises the following steps of: detecting the condition of tobacco mosaic virus (TMV) carried by tobacco seedlings 7 days before transplanting, and removing the tobacco seedlings of which the virus carrying rate is over 5 percent; spraying an organic antiviral agent (a volume ratio is 0.5-5 percent) once to the tobacco seedlings 1 to 2 days before transplanting, wherein 60 to 120 kilograms of organic antiviral agent is sprayed to one square meter of seedling raising plate; before transplanting, spraying the organic antiviral agent in a tobacco pond of a planting field, wherein 0.5 to 1.0 kilogram of organic antiviral agent is sprayed per mu; sorting the tobacco seedlings, removing weak seedlings, and transplanting the tobacco seedlings; and after transplanting, spraying the organic antiviral agent (the volume ratio is 0.5-5 percent) to the tobacco seedlings every 6 to 7 days, and spraying continuously for 3 to 4 times, wherein 15 to 40 kilograms of organic antiviral agent is sprayed per mu. According to the method, antiviral treatment is performed by combining the propagation characteristics of tobacco mosaic diseases in different phases from seedling raising management and the treatment of loam in the planting field to the management of the field planting period, and the tobacco seedlings which are transplanted to a field are treated by utilizing the organic antiviral agent to induce the tobacco seedlings to generate system disease resistance, so that the safety of tobacco planting is ensured, and an effect of preventing TMV is achieved.
Description
Technical field
The invention belongs to tobacco planting technical field, further belong to tobacco floating seed rearing technical field, be specifically related to a kind of method that tobacco floating seed rearing field planting phase tobacco mosaic occurs of preventing and treating.
Background technology
Tobacco mosaic virus (TMV,
tobacco mosaic virus), be the pathogene of tobacco mosaic etc., belong to Tobamovirus (
tobamovirus).TMV is that a kind of size is the upright and outspoken baculoviral of 300 nm × 18 nm, and nucleic acid is unimolecule positive chain RNA, and RNA is wrapped up by more than 2,000 constitutionally stable protein subunits, and its architectural characteristic has determined that TMV has high stability.It is reported, the TMV that in vitro low temperature is preserved is still keeping infecting activity after many decades, more than 10min could be by viral passivation under 90 DEG C of conditions.
Tobacco is one of plant of Solanaceae the most easily infecting TMV, and at present, the tobacco mosaic that TMV causes is planted cigarette area and extensively distributed in worldwide.The main illness of tobacco that infects TMV shows as: slight floral leaf, heavy floral leaf, bleb shape floral leaf and yellow.Infect TMV seedling stage and can cause cigarette strain to be downgraded and deformity, the easy necrotic spot that produces after the tobacco leaf of field period infection TMV is shone by the sun in the morbidity later stage and shines.Tobacco leaf the most outstanding performance after baking of infecting TMV is that blade attenuation, color and luster are thin out, i.e. all degradations of weight and quality; Interior quality analysis result shows: in the tobacco leaf of infection TMV, full nitrogen, full potassium and protein content obviously increase, and total reducing sugar, reducing sugar and schmuck value significantly decline.The serious field of falling ill in early days, the output that TMV causes and mass loss can be up to more than 90%.TMV host range is wide.Can infect 400 various plants of 36 sections such as Solanaceae, Cruciferae, Curcurbitaceae and pulse family.So, in vega and the plant sample adopted of vega periphery all likely become the primary source of infection of TMV, in addition, if the water source of the soil gathering in vega, periphery and seedling-cultivating tray and seedling medium are infected, also may become source of infection.Because TMV stability is high, distribution very extensively, infect that scope is very wide, route of transmission is diversified, cause the prevention and control of TMV very difficult, in addition the molecular variant of virus and the generation of drug resistance, the anti-virus formulation of selling on the upper use of production at present and market or the drug effect of medicine are degenerated gradually, can only prevent in a short time the infection of TMV and spreading of symptom, do not reach the object of effective control mosaic disease, once mosaic disease outburst, cannot fundamentally alleviate mosaic disease and produce the loss causing to tobacco.
TMV mainly infects by mechanical friction with band venom liquid, and field operates by sick seedling and strong seedling friction or farming the microtrauma mouth causing to be just enough to allow TMV infect again.Migrating of the biting mouthparts such as locust, oriental tobacco budworm insect also can be caused generally infection and the propagation of TMV.There are some researches show in some cigarette districts, the popular ascendant trend faster that is of generation of TMV, the incidence of some field tobacco mosaic virus diseases of High Quality Tobacco production area is up to more than 90%, mosaic disease serious threat to the safety in production of flue-cured tobacco, the social problems such as even cause that some tobacco grower colonies are complained to the higher authorities about an injustice and request fair settlement.
It is the committed step that tobacco produces that cigarette transplantation of seedlings enters field, and cigarette shoot root system is left in the process that seedbed enters field, because needs take out cigarette seedling artificially from seedbed, cause certain damage will to like this cigarette shoot root system.Cigarette transplantation of seedlings is to initial stage in land for growing field crops, and damage, the cigarette seedling of cigarette shoot root system survive not yet completely, and resistance against diseases is weak and plant under the impact of the multiple factors such as generality of cigarette environment band poison, is very easy to cause infecting, propagate and spreading of tobacco mosaic virus.Therefore, develop a kind of method that tobacco floating seed rearing field planting phase tobacco mosaic occurs of preventing and treating, very necessary for tobacco safety in production.
Summary of the invention
The object of the present invention is to provide a kind of method that tobacco floating seed rearing field planting phase tobacco mosaic occurs of preventing and treating.
The present invention seeks to realize like this, comprise transplant before detections of cigarette seedling diseases viral disease, transplanted seedling processing, planting site after processing and transplanting, manage, be specially:
A, in cigarette transplantation of seedlings first 7 days, carry TMV situation to cigarette seedling and detect, and exceedes cigarette seedling in 5% seedling-cultivating tray give it up with malicious rate;
B, transplanted seedling processing: organic antivirotic 1 time of spraying (volume ratio) 0.5 ~ 5% to cigarette seedling in 1 ~ 2 day before cigarette transplantation of seedlings, the amount of spraying is every hundred square metres of seedling-cultivating tray 60 ~ 120Kg;
C, planting site processing: before cigarette transplantation of seedlings, soil in the planting site cigarette pool is applied to organic antivirotic, formulation rate is 0.5 ~ 1.0 Kg/ mu;
D, cigarette transplantation of seedlings: cigarette seedling is sorted, reject weak seedling, then carry out cigarette transplantation of seedlings by technical specification;
E, transplant after management: within after cigarette transplantation of seedlings every 6 ~ 7 days, spray organic antivirotic 1 time of (volume ratio) 0.5 ~ 5% to cigarette seedling, the amount of spraying is 15 ~ 40Kg/ mu, sprays continuously 3 ~ 4 times.
Prior art shows, organic inductive antiviral agents can make plant obtain systemic disease resistance, is a kind of biological nature that plant opposing pathogen infects.That is to say, by some, biological or abiotic stimulus (inducer) induction produces system resistance new, wide spectrum to plant, thereby pathogen is infected and infecting of other germs all has very strong resistance, and this resistance level can expand to whole plant.Polypeptide treasured, AHO, molecular weight belong to organic inductive antiviral agents at chitosan, saikoside A or the ginkolide B of 500-1500, the precious main component of described polypeptide is Penicillium notatum inactivated bacteria filament, can produce system resistance against diseases by inducing plant, must fully contact with cigarette strain root, inhale by root the sexual element competence exertion effect of being indecisive and changeable, while therefore use, will admix in matrix; Described AHO is that 3-hydroxyl-3 acetonyl-indolones (3-acetonyl-3-hydroxyoxindole) can stimulate or inducing plant obtains systemic disease resistance biotic factor, AHO has stronger anti-TMV activity, find through further investigation, this compound is not to directly act on virus but the defense response of inducing plant, and this induction depends on SA signal pathway; Molecular weight is at the chitosan of 500-1500, and main component is (Isosorbide-5-Nitrae)-2-amino-2-deoxidation-callose, can produce system resistance against diseases by inducing plant.Saikoside A extracts from the root of the Bupleurum Bupleurum DC. of San Hang section plant, and ginkolide B extracts from ginkgo.
The present invention is in conjunction with the propagation characteristic of tobacco mosaic virus, process each different phase of field planting period management from seedling management, planting site loam and carry out antiviral processing, the cigarette seedling that utilizes organic inductive antivirotic to be transplanted to field planting is blocked mosaic virus infection opportunity, thereby ensure the safety of tobacco planting, reached the technique effect of prevention.
Embodiment
The present invention is further illustrated below, but never in any form the present invention is limited, and any change or the improvement done based on training centre of the present invention, all belong to protection scope of the present invention.
The present invention is characterized in that comprising the front cigarette seedling diseases viral disease detection of transplanting, transplanted seedling processing, planting site processing and transplants rear management, is specially:
A, in cigarette transplantation of seedlings first 7 days, carry TMV situation to cigarette seedling and detect, and exceedes cigarette seedling in 5% seedling-cultivating tray give it up with malicious rate;
B, transplanted seedling processing: organic antivirotic 1 time of spraying (volume ratio) 0.5 ~ 5% to cigarette seedling in 1 ~ 2 day before cigarette transplantation of seedlings, the amount of spraying is every hundred square metres of seedling-cultivating tray 60 ~ 120Kg;
C, planting site processing: before cigarette transplantation of seedlings, to applying organic antivirotic in the planting site cigarette pool, formulation rate is 0.5 ~ 1.0 Kg/ mu;
D, cigarette transplantation of seedlings: cigarette seedling is sorted, reject weak seedling, then carry out cigarette transplantation of seedlings by technical specification;
E, transplant after management: within after cigarette transplantation of seedlings every 6 ~ 7 days, spray organic antivirotic 1 time of (volume ratio) 0.5 ~ 5% to cigarette seedling, the amount of spraying is 15 ~ 40Kg/ mu, sprays continuously 3 ~ 4 times.
As preferred embodiment:
The method of detection TMV described in A step is to extract 1 ~ 2% cigarette seedling of cigarette seedling sum by five-spot, adopts enzyme-linked immunosorbent assay to carry detection TMV situation to cigarette seedling and detects.
Transplanting and organic antivirotic described in D, E step spray in process, opponent and instrument disinfection, the thimerosal using is the clorox of the skim milk of (weight ratio) 5 ~ 20% and the Tween 20 of (weight ratio) 0.1 ~ 1% or (weight ratio) 0.1 ~ 1.0%.
Described organic antivirotic is organic inductive antivirotic.
Organic antivirotic described in B step is AHO or chitosan; Organic antivirotic described in C step is polypeptide treasured or AHO; Organic antivirotic described in E step is AHO, chitosan, saikoside A or ginkolide B; The molecular weight of described chitosan is 500 ~ 1500.
Described organic antivirotic is aqua, emulsion, granule, wetting powder, water dispersible granules.
Embodiment 1
In cigarette transplantation of seedlings first 7 days, cigarette seedling is extracted to 1% cigarette seedling of cigarette seedling sum by five-spot, adopt enzyme-linked immunosorbent assay to carry and detect TMV situation and detect cigarette seedling, exceed cigarette seedling in 5% seedling-cultivating tray with malicious rate and give it up.The AHO that sprays 1 (volume ratio) 0.5% to cigarette seedling in first 1 day in cigarette transplantation of seedlings, the amount of spraying is every hundred square metres of seedling-cultivating tray 60Kg; Before cigarette transplantation of seedlings, to applying polypeptide treasured in the planting site cigarette pool, formulation rate is 0.5Kg/ mu; Before cigarette transplantation of seedlings, reply cigarette seedling sorts, and rejects weak seedling, then carries out cigarette transplantation of seedlings by technical specification; The chitosan that the molecular weight of spraying 1 (volume ratio) 0.5% to cigarette seedling in every 6 days after cigarette transplantation of seedlings is 500 ~ 1500, the amount of spraying is 15Kg/ mu, sprays continuously 3 times.Described transplanting and organic antivirotic spray in process, opponent and instrument disinfection, and the thimerosal using is the skim milk of (weight ratio) 5% and the Tween 20 of (weight ratio) 0.1%.
Embodiment 2
In cigarette transplantation of seedlings first 7 days, cigarette seedling is extracted to 2% cigarette seedling of cigarette seedling sum by five-spot, adopt enzyme-linked immunosorbent assay to carry and detect TMV situation and detect cigarette seedling, exceed cigarette seedling in 5% seedling-cultivating tray with malicious rate and give it up.The chitosan that the molecular weight of spraying (volume ratio) 5% to cigarette seedling in first 2 days in cigarette transplantation of seedlings is 500 ~ 1,500 1 time, the amount of spraying is every hundred square metres of seedling-cultivating tray 120Kg; Before cigarette transplantation of seedlings, to applying AHO in the planting site cigarette pool, formulation rate is 1.0 Kg/ mus; Before cigarette transplantation of seedlings, reply cigarette seedling sorts, and rejects weak seedling, then carries out cigarette transplantation of seedlings by technical specification; The saikoside A that sprays 1 (volume ratio) 5% to cigarette seedling in every 7 days after cigarette transplantation of seedlings, the amount of spraying is 40Kg/ mu, sprays continuously 4 times.Described transplanting and organic antivirotic spray in process, opponent and instrument disinfection, and the thimerosal using is the skim milk of (weight ratio) 20% and the Tween 20 of (weight ratio) 1%.
Embodiment 3
In cigarette transplantation of seedlings first 7 days, cigarette seedling is extracted to 1% cigarette seedling of cigarette seedling sum by five-spot, adopt enzyme-linked immunosorbent assay to carry and detect TMV situation and detect cigarette seedling, exceed cigarette seedling in 5% seedling-cultivating tray with malicious rate and give it up.The AHO that sprays 1 (volume ratio) 1% to cigarette seedling in first 1 day in cigarette transplantation of seedlings, the amount of spraying is every hundred square metres of seedling-cultivating tray 70Kg; Before cigarette transplantation of seedlings, to applying polypeptide treasured in the planting site cigarette pool, formulation rate is 0.7 Kg/ mu; Before cigarette transplantation of seedlings, reply cigarette seedling sorts, and rejects weak seedling, then carries out cigarette transplantation of seedlings by technical specification; The AHO that sprays 1 (volume ratio) 1% to cigarette seedling in every 6 days after cigarette transplantation of seedlings, the amount of spraying is 30Kg/ mu, sprays continuously 3 times.Described transplanting and organic antivirotic spray in process, opponent and instrument disinfection, and the thimerosal using is the skim milk of (weight ratio) 15% and the clorox of (weight ratio) 0.1%.
Embodiment 4
In cigarette transplantation of seedlings first 7 days, cigarette seedling is extracted to 2% cigarette seedling of cigarette seedling sum by five-spot, adopt enzyme-linked immunosorbent assay to carry and detect TMV situation and detect cigarette seedling, exceed cigarette seedling in 5% seedling-cultivating tray with malicious rate and give it up.The chitosan that the molecular weight of spraying (volume ratio) 2% to cigarette seedling in first 2 days in cigarette transplantation of seedlings is 500 ~ 1,500 1 time, the amount of spraying is every hundred square metres of seedling-cultivating tray 80Kg; Before cigarette transplantation of seedlings, to applying AHO in the planting site cigarette pool, formulation rate is 0.8 Kg/ mu; Before cigarette transplantation of seedlings, reply cigarette seedling sorts, and rejects weak seedling, then carries out cigarette transplantation of seedlings by technical specification; The ginkolide B of spraying 1 (volume ratio) 2% to cigarette seedling in every 7 days after cigarette transplantation of seedlings, the amount of spraying is 25Kg/ mu, sprays continuously 4 times.Described transplanting and organic antivirotic spray in process, opponent and instrument disinfection, and the thimerosal using is the skim milk of (weight ratio) 10% and the clorox of (weight ratio) 0.5%.
Embodiment 5
In cigarette transplantation of seedlings first 7 days, cigarette seedling is extracted to 1% cigarette seedling of cigarette seedling sum by five-spot, adopt enzyme-linked immunosorbent assay to carry and detect TMV situation and detect cigarette seedling, exceed cigarette seedling in 5% seedling-cultivating tray with malicious rate and give it up.The AHO that sprays 1 (volume ratio) 3% to cigarette seedling in first 1 day in cigarette transplantation of seedlings, the amount of spraying is every hundred square metres of seedling-cultivating tray 90Kg; Before cigarette transplantation of seedlings, to applying polypeptide treasured in the planting site cigarette pool, formulation rate is 0.9Kg/ mu; Before cigarette transplantation of seedlings, reply cigarette seedling sorts, and rejects weak seedling, then carries out cigarette transplantation of seedlings by technical specification; The chitosan that the molecular weight of spraying 1 (volume ratio) 3% to cigarette seedling in every 6 days after cigarette transplantation of seedlings is 500 ~ 1500, the amount of spraying is 35Kg/ mu, sprays continuously 3 times.Described transplanting and organic antivirotic spray in process, opponent and instrument disinfection, and the thimerosal using is the skim milk of (weight ratio) 15% and the Tween 20 of (weight ratio) 0.5%.
Embodiment 6
In cigarette transplantation of seedlings first 7 days, cigarette seedling is extracted to 2% cigarette seedling of cigarette seedling sum by five-spot, adopt enzyme-linked immunosorbent assay to carry and detect TMV situation and detect cigarette seedling, exceed cigarette seedling in 5% seedling-cultivating tray with malicious rate and give it up.The chitosan that the molecular weight of spraying (volume ratio) 4% to cigarette seedling in first 2 days in cigarette transplantation of seedlings is 500 ~ 1,500 1 time, the amount of spraying is every hundred square metres of seedling-cultivating tray 100Kg; Before cigarette transplantation of seedlings, to applying AHO in the planting site cigarette pool, formulation rate is 0.6Kg/ mu; Before cigarette transplantation of seedlings, reply cigarette seedling sorts, and rejects weak seedling, then carries out cigarette transplantation of seedlings by technical specification; The ginkolide B of spraying 1 (volume ratio) 4% to cigarette seedling in every 7 days after cigarette transplantation of seedlings, the amount of spraying is 33Kg/ mu, sprays continuously 4 times.Described transplanting and organic antivirotic spray in process, opponent and instrument disinfection, and the thimerosal using is the skim milk of (weight ratio) 15% and the clorox of (weight ratio) 0.5%.
The on-the-spot comparative trial of carrying out for above-described embodiment.
Test period: in July, 2010 is to November
Test site: experiment station of Yunnan Tobacco Company Kunming Branch
Test kind: the large gold dollar of safflower
The control group 7 of transplanting according to embodiment 1 ~ 6 set handling group 1 ~ 6 and usual manner.Sample and TMV method for detecting virus seedling stage: the same day cigarette seedling of all processing is sampled in transplanting, 22-24 sample extracted in each processing, wherein 330 samples of processed group, and 331 of control groups, extract altogether 661 samples.All adopt ELISA to detect.
Field investigation method: spray first by the end of September medicine, spray in every 6 ~ 7 days 1 time, continuous 4 times.The investigation on the same day of dispenser for the second time morbidity radix, every 1 state of an illness of investigation in 10 days, field period is investigated 5 times altogether later.Investigation Recording criteria is according to carrying out severity Scaling by " the insect pest classification of GB-T 23222-2008 tobacco disease and investigation method ", calculating the incidence of disease and disease index.Control efficiency is calculated according to following formula.
Data processing method: field data all adopts Excel and DPS software to arrange, analyze, carries out standardization conversion (100-disease refers to) to sick index certificate before variance analysis, and numerical value is larger, and the state of an illness is lighter.
Production management measure: mid-July sowing, late August thinning, final singling, leaf-cutting by the end of August the 1st time, sprays the 1st time before cutting seedling, sprays 1 time totally three times before cutting seedling at every turn.When transplanting, reject the cigarette strain that has TMV classical symptom, plant and carry out intertill and clean tillage in latter 33 days, plant cigarette strain in latter 40 days and enter prosperous long-term.
The each control efficiency (unit: %) contrasting with clear water of processing of table 1. processed group
| Processing mode | 12d after planting | 19d after planting | 26d after planting | 1 plants rear 33d | 40d after planting |
| Processed group 1 | 98.16 | 72.18 | 63.91 | 58.95 | 47.21 |
| Processed group 2 | 95.18 | 78.19 | 71.66 | 69.53 | 52.29 |
| Processed group 3 | 69.81 | 56.21 | 50.78 | 48.23 | 38.91 |
| Processed group 4 | 71.14 | 59.65 | 41.47 | 39.17 | 31.62 |
| Processed group 5 | 74.88 | 63.69 | 54.37 | 48.29 | 38.12 |
| Processed group 6 | 81.13 | 69.64 | 51.98 | 49.82 | 31.65 |
| Control group 7(disease refers to) | 11.02 | 33.66 | 34.57 | 57.02 | 67.12 |
Note: in table, "+" represents to increase, "-" represents to reduce.Lower same.
Table 2. is planted the significance of difference analysis (LSD method) of the rear anti-TMV of 40d each processed group cigarette strain
| Processing mode | Average | 5% significance level | 1% utmost point significance level |
| Processed group 1 | 70.61 | a | A |
| Processed group 2 | 69.78 | a | AB |
| Processed group 3 | 56.69 | ab | ABC |
| Processed group 4 | 60.27 | ab | ABC |
| Processed group 5 | 62.41 | ab | ABC |
| Processed group 6 | 66.35 | ab | ABC |
| Control group 7 | 32.88 | " bcde" | "BCDE" |
Claims (6)
1. prevent and treat a method for tobacco floating seed rearing field planting phase tobacco mosaic generation, it is characterized in that comprising and transplant front cigarette seedling diseases viral disease detection, transplanted seedling processing, planting site processing and transplant rear management, be specially:
A, in cigarette transplantation of seedlings first 7 days, carry TMV situation to cigarette seedling and detect, and exceedes cigarette seedling in 5% seedling-cultivating tray give it up with malicious rate;
B, transplanted seedling processing: organic antivirotic 1 time of spraying volume ratio 0.5 ~ 5% to cigarette seedling in 1 ~ 2 day before cigarette transplantation of seedlings, this organic antivirotic is AHO or chitosan, the amount of spraying is every hundred square metres of seedling-cultivating tray 60 ~ 120Kg;
C, planting site processing: before cigarette transplantation of seedlings, to applying organic antivirotic in the planting site cigarette pool, this organic antivirotic is polypeptide treasured or AHO, and formulation rate is 0.5 ~ 1.0Kg/ mu;
D, cigarette transplantation of seedlings: cigarette seedling is sorted, reject weak seedling, then carry out cigarette transplantation of seedlings by technical specification;
E, transplant after management: within after cigarette transplantation of seedlings every 6 ~ 7 days, spray organic antivirotic 1 time of volume ratio 0.5 ~ 5% to cigarette seedling, this organic antivirotic is AHO, chitosan, saikoside A or ginkolide B, and the amount of spraying is 15 ~ 40Kg/ mu, sprays continuously 3 ~ 4 times.
2. the method that control tobacco floating seed rearing field planting phase tobacco mosaic according to claim 1 occurs, it is characterized in that: the method for the detection TMV described in A step is to extract 1 ~ 2% cigarette seedling of cigarette seedling sum by five-spot, adopt enzyme-linked immunosorbent assay to carry detection TMV situation to cigarette seedling and detect.
3. the method that control tobacco floating seed rearing field planting phase tobacco mosaic according to claim 1 occurs, it is characterized in that: transplanting and organic antivirotic described in D, E step spray in process, opponent and instrument disinfection, the clorox of the skim milk that the thimerosal using is weight ratio 5 ~ 20% and the Tween20 of weight ratio 0.1 ~ 1% or weight ratio 0.1 ~ 1%.
4. the method that control tobacco floating seed rearing field planting phase tobacco mosaic according to claim 1 occurs, is characterized in that: described organic antivirotic is organic inductive antivirotic.
5. the method that control tobacco floating seed rearing field planting phase tobacco mosaic according to claim 1 occurs, is characterized in that: the molecular weight of described chitosan is 500 ~ 1500.
6. the method occurring according to the control tobacco floating seed rearing field planting phase tobacco mosaic described in claim 1,3 or 4, is characterized in that: described organic antivirotic is aqua, emulsion, granule, wetting powder, water dispersible granules.
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| CN105746321B (en) * | 2016-03-22 | 2019-01-22 | 中国烟草总公司重庆市公司巫溪分公司 | Reduce the tobacco floating seed rearing method of seedbed disease spread |
| CN108781931A (en) * | 2017-06-02 | 2018-11-13 | 潍坊市烟草专卖局 | A kind of green control method of tobacco planting pest and disease damage |
| CN108863538A (en) * | 2018-07-18 | 2018-11-23 | 合肥百绿盛农业科技有限公司 | A kind of plant growth regulator for tomato and grape |
| CN109042658A (en) * | 2018-07-18 | 2018-12-21 | 合肥百绿盛农业科技有限公司 | The application method and its application of plant growth regulator composition |
| CN108782623A (en) * | 2018-07-18 | 2018-11-13 | 合肥百绿盛农业科技有限公司 | Efficient Yield increasing type plant growth regulator and its application |
| CN108739889A (en) * | 2018-07-18 | 2018-11-06 | 合肥百绿盛农业科技有限公司 | A kind of sweet osmanthus nursery stock plant growth regulator |
| CN108617713A (en) * | 2018-07-18 | 2018-10-09 | 合肥百绿盛农业科技有限公司 | A kind of preparation method of nuisanceless composite plant growth regulator |
| CN110089320A (en) * | 2019-04-11 | 2019-08-06 | 饶超奇 | A kind of application method and its application of chitosan solution |
| CN110537470A (en) * | 2019-05-22 | 2019-12-06 | 贵州省烟草公司黔南州公司 | comprehensive prevention and control technology for tobacco rootstock diseases |
| CN116195602A (en) * | 2022-12-05 | 2023-06-02 | 湖南省烟草公司永州市公司 | Composite medicament for preventing and treating tobacco common mosaic disease as well as preparation method and application thereof |
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| NZ213370A (en) * | 1985-01-16 | 1989-03-29 | Vira Tek Inc | Method of treating viral infections of selected trees and field crops using ribavirin (1-b-d-ribofuranosyl-1,2,4-triazole-3-carboxamide) or the 2,3,5-triacetyl or 5'-butyryl derivative thereof |
| CN1332137A (en) * | 2001-05-25 | 2002-01-23 | 刘爱军 | Foliage-spraying special tobacco fertilizer |
| CN100586286C (en) * | 2006-06-09 | 2010-02-03 | 姚宇澄 | Plant antiviral agent, preparation method and application thereof |
| CN101601354B (en) * | 2009-07-13 | 2010-12-08 | 西南大学 | Method for preventing and controlling mosaic disease based on improving tobacco plant nutrition resistance |
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