CN102696413A - Method for preventing and controlling tobacco mosaic diseases in field planting period of tobacco floating seedling raising - Google Patents
Method for preventing and controlling tobacco mosaic diseases in field planting period of tobacco floating seedling raising Download PDFInfo
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Abstract
The invention discloses a method for preventing and controlling tobacco mosaic diseases in field planting period of tobacco floating seedling raising. The method comprises the following steps of: detecting the condition of tobacco mosaic virus (TMV) carried by tobacco seedlings 7 days before transplanting, and removing the tobacco seedlings of which the virus carrying rate is over 5 percent; spraying an organic antiviral agent (a volume ratio is 0.5-5 percent) once to the tobacco seedlings 1 to 2 days before transplanting, wherein 60 to 120 kilograms of organic antiviral agent is sprayed to one square meter of seedling raising plate; before transplanting, spraying the organic antiviral agent in a tobacco pond of a planting field, wherein 0.5 to 1.0 kilogram of organic antiviral agent is sprayed per mu; sorting the tobacco seedlings, removing weak seedlings, and transplanting the tobacco seedlings; and after transplanting, spraying the organic antiviral agent (the volume ratio is 0.5-5 percent) to the tobacco seedlings every 6 to 7 days, and spraying continuously for 3 to 4 times, wherein 15 to 40 kilograms of organic antiviral agent is sprayed per mu. According to the method, antiviral treatment is performed by combining the propagation characteristics of tobacco mosaic diseases in different phases from seedling raising management and the treatment of loam in the planting field to the management of the field planting period, and the tobacco seedlings which are transplanted to a field are treated by utilizing the organic antiviral agent to induce the tobacco seedlings to generate system disease resistance, so that the safety of tobacco planting is ensured, and an effect of preventing TMV is achieved.
Description
Technical field
The invention belongs to the tobacco planting technical field, further belong to the tobacco floating seed rearing technical field, be specifically related to a kind of method that tobacco floating seed rearing field planting phase tobacco mosaic takes place of preventing and treating.
Background technology
Tobacco mosaic virus (TMV,
Tobacco mosaic virus), be the pathogene of tobacco mosaic etc., belong to Tobamovirus (
Tobamovirus).TMV is that a kind of size is the upright and outspoken baculoviral of 300 nm * 18 nm, and nucleic acid is the unimolecule positive chain RNA, and RNA is by more than 2,000 constitutionally stable protein subunit parcels, and its architectural characteristic has determined TMV to have high stability.It is reported that the TMV that the low temperature that exsomatizes is preserved is still keeping infecting activity behind many decades, could be with viral passivation more than the 10min under 90 ℃ of conditions.
Tobacco is one of plant of Solanaceae that infects the most easily TMV, and at present, the tobacco mosaic that TMV causes is planted the cigarette area and extensively distributed in worldwide.The main illness of tobacco that infects TMV shows as: slight floral leaf, heavy floral leaf, bleb shape floral leaf and yellow.Infect TMV seedling stage and can cause the cigarette strain to be downgraded and deformity, the tobacco leaf that field period infects TMV is easy to generate necrotic spot by the sun in the morbidity later stage after shining.Tobacco leaf the most outstanding performance after baking of infecting TMV is that blade attenuation, color and luster are thin out, and promptly weight and quality all seriously descend; The interior quality analysis result shows: full nitrogen, full potassium and protein content obviously increase in the tobacco leaf of infection TMV, and total reducing sugar, reducing sugar and schmuck value significantly descend.The field piece of falling ill serious in early days, output that TMV causes and mass loss can be up to more than 90%.The TMV host range is wide.Can infect 400 various plants of 36 sections such as Solanaceae, Cruciferae, Curcurbitaceae and pulse family.So, reach the primary source of infection that the peripheral plant sample of being adopted of vega all might become TMV in the vega, in addition, the water source of the soil of gathering in the vega, periphery and seedling-cultivating tray and seedling medium also possibly become source of infection if infect.Since high, the distribution of TMV stability very extensively, infect that scope is very wide, the route of transmission is diversified; Caused the prevention and control of TMV very difficult; The molecular variant and the chemical sproof generation of virus in addition; Produce at present go up use with market on the anti-virus formulation sold or the drug effect degeneration gradually of medicine, can only prevent the infection of TMV and spreading of symptom in a short time, do not reach the purpose of effective control mosaic disease; Mosaic disease will be in case outburst can't fundamentally alleviate the loss that mosaic disease causes to tobacco production.
TMV mainly infects through mechanical friction with band venom liquid, and the microtrauma mouth that causes is operated through sick seedling and strong seedling friction or farming in the field just to be enough to let TMV infect again.Migrating of biting mouthparts such as locust, oriental tobacco budworm insect also can be caused generally infection and the propagation of TMV.There are some researches show in some cigarette districts; The popular ascendant trend faster that is of the generation of TMV; The incidence of some field piece tobacco mosaic virus diseases of high-quality cigarette production area is up to more than 90%, mosaic disease serious threat to the safety in production of flue-cured tobacco, even social problems such as cause that some tobacco grower colonies are complained to the higher authorities about an injustice and request fair settlement.
It is the committed step that tobacco produces that the field is gone in the cigarette transplantation of seedlings, and cigarette shoot root system is left in the process in entering field, seedbed, owing to need the cigarette seedling be taken out from the seedbed artificially, causes certain damage will for like this cigarette shoot root system.The cigarette transplantation of seedlings is to initial stage in land for growing field crops, and the damage of cigarette shoot root system, cigarette seedling become to live as yet fully, and resistance against diseases is weak and plant under the influence of multiple factors such as generality of cigarette environment band poison, is very easy to cause infecting, propagate and spreading of tobacco mosaic virus.Therefore, develop a kind of method that tobacco floating seed rearing field planting phase tobacco mosaic takes place of preventing and treating, very necessary for the tobacco safety in production.
Summary of the invention
The object of the present invention is to provide a kind of method that tobacco floating seed rearing field planting phase tobacco mosaic takes place of preventing and treating.
The present invention seeks to realize like this, comprise transplant before cigarette seedling diseases viral disease detect, transplanted seedling is handled, planting site is handled and transplant the back management, be specially:
A, in the cigarette transplantation of seedlings preceding 7 days carry the TMV situation to the cigarette seedling and detect, and surpass in 5% the seedling-cultivating tray cigarette seedling with malicious rate and give it up;
B, transplanted seedling handle: before the cigarette transplantation of seedlings, gave organic antivirotic 1 time that the cigarette seedling sprays (volume ratio) 0.5 ~ 5% in 1 ~ 2 day, the amount of spraying is per hundred square metres of seedling-cultivating tray 60 ~ 120Kg;
C, planting site are handled: before the cigarette transplantation of seedlings, soil in the planting site cigarette pool is applied organic antivirotic, formulation rate is 0.5 ~ 1.0 Kg/ mu;
D, cigarette transplantation of seedlings: the cigarette seedling is sorted, reject weak seedling, carry out the cigarette transplantation of seedlings by technical specification then;
E, transplant the back management: gave organic antivirotic 1 time that the cigarette seedling sprays (volume ratio) 0.5 ~ 5% after the cigarette transplantation of seedlings in per 6 ~ 7 days, the amount of spraying is 15 ~ 40Kg/ mu, sprays continuously 3 ~ 4 times.
Prior art shows that the organic inductive antiviral agents can make plant obtain systemic disease resistance, is a kind of biological nature that plant opposing pathogen infects.That is to say, plant by some biological or abiotic stimulus (inducer) induce and produce system new, wide spectrum resistance, thereby pathogen is infected and infecting of other germs all has very strong resistance, this resistance level can expand to whole plant.Polypeptide treasured, AHO, molecular weight belong to the organic inductive antiviral agents at chitosan, saikoside A or the ginkolide B of 500-1500; The precious main component of described polypeptide is a Penicillium notatum inactivated bacteria filament; Can produce system's resistance against diseases by inducing plant; Must fully contact with cigarette strain root, inhale the sexual element competence exertion effect of being indecisive and changeable, will admix in the matrix when therefore using through root; Described AHO is that 3-hydroxyl-3 acetonyls-indolone (3-acetonyl-3-hydroxyoxindole) can stimulate or inducing plant obtains the systemic disease resistance biotic factor; It is active that AHO has stronger anti-TMV; Find through further investigation; This compound is not to directly act on virus but the defense response of inducing plant, and this inducing depends on the SA signal pathway; Molecular weight is at the chitosan of 500-1500, and main component is (1,4)-2-amino-2-deoxidation-callose, can produce system's resistance against diseases by inducing plant.Saikoside A extracts from the root of the Bupleurum Bupleurum DC. of the capable section of umbrella plant, and ginkolide B extracts from ginkgo.
The present invention combines the propagation characteristic of tobacco mosaic virus; Handle each different phase of field planting period management from seedling management, planting site loam and carry out antiviral processing; Utilize the cigarette seedling that the organic inductive antivirotic is transplanted to field planting that the mosaic virus infection is blocked opportunity; Thereby guaranteed the safety of tobacco planting, reached the technique effect of prevention.
Embodiment
Be further described in the face of the present invention down, but never in any form the present invention limited, any change or improvement based on training centre of the present invention is done all belong to protection scope of the present invention.
The present invention manages after it is characterized in that comprising the preceding cigarette seedling diseases viral disease detection of transplanting, transplanted seedling processing, planting site processing and transplanting, is specially:
A, in the cigarette transplantation of seedlings preceding 7 days carry the TMV situation to the cigarette seedling and detect, and surpass in 5% the seedling-cultivating tray cigarette seedling with malicious rate and give it up;
B, transplanted seedling handle: before the cigarette transplantation of seedlings, gave organic antivirotic 1 time that the cigarette seedling sprays (volume ratio) 0.5 ~ 5% in 1 ~ 2 day, the amount of spraying is per hundred square metres of seedling-cultivating tray 60 ~ 120Kg;
C, planting site are handled: before the cigarette transplantation of seedlings, to applying organic antivirotic in the planting site cigarette pool, formulation rate is 0.5 ~ 1.0 Kg/ mu;
D, cigarette transplantation of seedlings: the cigarette seedling is sorted, reject weak seedling, carry out the cigarette transplantation of seedlings by technical specification then;
E, transplant the back management: gave organic antivirotic 1 time that the cigarette seedling sprays (volume ratio) 0.5 ~ 5% after the cigarette transplantation of seedlings in per 6 ~ 7 days, the amount of spraying is 15 ~ 40Kg/ mu, sprays continuously 3 ~ 4 times.
As preferred implementation:
The method of the described detection of A step TMV is to extract 1 ~ 2% cigarette seedling of cigarette seedling sum by five-spot, adopts enzyme-linked immunosorbent assay that the cigarette seedling is carried detection TMV situation and detects.
D, the described transplanting of E step and organic antivirotic spray in the process; Adversary and instrument disinfection, employed thimerosal are skim milk and the Tween 20 of (weight ratio) 0.1 ~ 1% or the clorox of (weight ratio) 0.1 ~ 1.0% of (weight ratio) 5 ~ 20%.
Described organic antivirotic is the organic inductive antivirotic.
The described organic antivirotic of B step is AHO or chitosan; The described organic antivirotic of C step is polypeptide treasured or AHO; The described organic antivirotic of E step is AHO, chitosan, saikoside A or ginkolide B; The molecular weight of described chitosan is 500 ~ 1500.
Described organic antivirotic is aqua, emulsion, granule, wetting powder, water dispersible granules.
Embodiment 1
In the cigarette transplantation of seedlings preceding 7 days, the cigarette seedling is extracted 1% cigarette seedling of cigarette seedling sum by five-spot, adopt enzyme-linked immunosorbent assay that the cigarette seedling is carried and detect the TMV situation and detect, surpass in 5% the seedling-cultivating tray cigarette seedling with malicious rate and give it up.At the AHO that the cigarette transplantation of seedlings sprayed 1 (volume ratio) 0.5% for the cigarette seedling in preceding 1 day, the amount of spraying is per hundred square metres of seedling-cultivating tray 60Kg; Before the cigarette transplantation of seedlings, to applying the polypeptide treasured in the planting site cigarette pool, formulation rate is a 0.5Kg/ mu; Reply cigarette seedling sorts before the cigarette transplantation of seedlings, rejects weak seedling, carries out the cigarette transplantation of seedlings by technical specification then; The molecular weight of spraying 1 (volume ratio) 0.5% for the cigarette seedling in per 6 days after the cigarette transplantation of seedlings is 500 ~ 1500 chitosan, and the amount of spraying is a 15Kg/ mu, sprays continuously 3 times.Described transplanting and organic antivirotic spray in the process, adversary and instrument disinfection, and employed thimerosal is the skim milk of (weight ratio) 5% and the Tween 20 of (weight ratio) 0.1%.
Embodiment 2
In the cigarette transplantation of seedlings preceding 7 days, the cigarette seedling is extracted 2% cigarette seedling of cigarette seedling sum by five-spot, adopt enzyme-linked immunosorbent assay that the cigarette seedling is carried and detect the TMV situation and detect, surpass in 5% the seedling-cultivating tray cigarette seedling with malicious rate and give it up.The molecular weight of spraying (volume ratio) 5% for the cigarette seedling preceding 2 days of cigarette transplantation of seedlings is 500 ~ 1500 chitosan 1 time, and the amount of spraying is per hundred square metres of seedling-cultivating tray 120Kg; Before the cigarette transplantation of seedlings, to applying AHO in the planting site cigarette pool, formulation rate is 1.0 Kg/ mus; Reply cigarette seedling sorts before the cigarette transplantation of seedlings, rejects weak seedling, carries out the cigarette transplantation of seedlings by technical specification then; The saikoside A that sprayed 1 (volume ratio) 5% for the cigarette seedling in per 7 days after the cigarette transplantation of seedlings, the amount of spraying is a 40Kg/ mu, sprays continuously 4 times.Described transplanting and organic antivirotic spray in the process, adversary and instrument disinfection, and employed thimerosal is the skim milk of (weight ratio) 20% and the Tween 20 of (weight ratio) 1%.
Embodiment 3
In the cigarette transplantation of seedlings preceding 7 days, the cigarette seedling is extracted 1% cigarette seedling of cigarette seedling sum by five-spot, adopt enzyme-linked immunosorbent assay that the cigarette seedling is carried and detect the TMV situation and detect, surpass in 5% the seedling-cultivating tray cigarette seedling with malicious rate and give it up.At the AHO that the cigarette transplantation of seedlings sprayed 1 (volume ratio) 1% for the cigarette seedling in preceding 1 day, the amount of spraying is per hundred square metres of seedling-cultivating tray 70Kg; Before the cigarette transplantation of seedlings, to applying the polypeptide treasured in the planting site cigarette pool, formulation rate is 0.7 Kg/ mu; Reply cigarette seedling sorts before the cigarette transplantation of seedlings, rejects weak seedling, carries out the cigarette transplantation of seedlings by technical specification then; The AHO that sprayed 1 (volume ratio) 1% for the cigarette seedling in per 6 days after the cigarette transplantation of seedlings, the amount of spraying is a 30Kg/ mu, sprays continuously 3 times.Described transplanting and organic antivirotic spray in the process, adversary and instrument disinfection, and employed thimerosal is the skim milk of (weight ratio) 15% and the clorox of (weight ratio) 0.1%.
Embodiment 4
In the cigarette transplantation of seedlings preceding 7 days, the cigarette seedling is extracted 2% cigarette seedling of cigarette seedling sum by five-spot, adopt enzyme-linked immunosorbent assay that the cigarette seedling is carried and detect the TMV situation and detect, surpass in 5% the seedling-cultivating tray cigarette seedling with malicious rate and give it up.The molecular weight of spraying (volume ratio) 2% for the cigarette seedling preceding 2 days of cigarette transplantation of seedlings is 500 ~ 1500 chitosan 1 time, and the amount of spraying is per hundred square metres of seedling-cultivating tray 80Kg; Before the cigarette transplantation of seedlings, to applying AHO in the planting site cigarette pool, formulation rate is 0.8 Kg/ mu; Reply cigarette seedling sorts before the cigarette transplantation of seedlings, rejects weak seedling, carries out the cigarette transplantation of seedlings by technical specification then; The ginkolide B of spraying 1 (volume ratio) 2% for the cigarette seedling in per 7 days after the cigarette transplantation of seedlings, the amount of spraying is a 25Kg/ mu, sprays continuously 4 times.Described transplanting and organic antivirotic spray in the process, adversary and instrument disinfection, and employed thimerosal is the skim milk of (weight ratio) 10% and the clorox of (weight ratio) 0.5%.
Embodiment 5
In the cigarette transplantation of seedlings preceding 7 days, the cigarette seedling is extracted 1% cigarette seedling of cigarette seedling sum by five-spot, adopt enzyme-linked immunosorbent assay that the cigarette seedling is carried and detect the TMV situation and detect, surpass in 5% the seedling-cultivating tray cigarette seedling with malicious rate and give it up.At the AHO that the cigarette transplantation of seedlings sprayed 1 (volume ratio) 3% for the cigarette seedling in preceding 1 day, the amount of spraying is per hundred square metres of seedling-cultivating tray 90Kg; Before the cigarette transplantation of seedlings, to applying the polypeptide treasured in the planting site cigarette pool, formulation rate is a 0.9Kg/ mu; Reply cigarette seedling sorts before the cigarette transplantation of seedlings, rejects weak seedling, carries out the cigarette transplantation of seedlings by technical specification then; The molecular weight of spraying 1 (volume ratio) 3% for the cigarette seedling in per 6 days after the cigarette transplantation of seedlings is 500 ~ 1500 chitosan, and the amount of spraying is a 35Kg/ mu, sprays continuously 3 times.Described transplanting and organic antivirotic spray in the process, adversary and instrument disinfection, and employed thimerosal is the skim milk of (weight ratio) 15% and the Tween 20 of (weight ratio) 0.5%.
Embodiment 6
In the cigarette transplantation of seedlings preceding 7 days, the cigarette seedling is extracted 2% cigarette seedling of cigarette seedling sum by five-spot, adopt enzyme-linked immunosorbent assay that the cigarette seedling is carried and detect the TMV situation and detect, surpass in 5% the seedling-cultivating tray cigarette seedling with malicious rate and give it up.The molecular weight of spraying (volume ratio) 4% for the cigarette seedling preceding 2 days of cigarette transplantation of seedlings is 500 ~ 1500 chitosan 1 time, and the amount of spraying is per hundred square metres of seedling-cultivating tray 100Kg; Before the cigarette transplantation of seedlings, to applying AHO in the planting site cigarette pool, formulation rate is a 0.6Kg/ mu; Reply cigarette seedling sorts before the cigarette transplantation of seedlings, rejects weak seedling, carries out the cigarette transplantation of seedlings by technical specification then; The ginkolide B of spraying 1 (volume ratio) 4% for the cigarette seedling in per 7 days after the cigarette transplantation of seedlings, the amount of spraying is a 33Kg/ mu, sprays continuously 4 times.Described transplanting and organic antivirotic spray in the process, adversary and instrument disinfection, and employed thimerosal is the skim milk of (weight ratio) 15% and the clorox of (weight ratio) 0.5%.
The on-the-spot comparative trial of carrying out to the foregoing description.
Test period: in July, 2010 is to November
Test place: Yunnan Tobacco Company Kunming Branch experiment station
Test kind: the big gold dollar of safflower
The control group 7 of processed group 1 ~ 6 and usual manner transplanting is set according to embodiment 1 ~ 6.Seedling stage sampling and TMV method for detecting virus: the same day cigarette seedling of all processing is sampled transplanting, each is handled and extracts 22-24 kind, 330 appearance of processed group wherein, and 331 of control groups extract 661 appearance altogether.All adopt ELISA to detect.
Field investigation method: spray medicine by the end of September first, spray in per 6 ~ 7 days 1 time, continuous 4 times.The investigation on the same day of dispenser for the second time morbidity radix, whenever at a distance from 1 state of an illness of investigation in 10 days, field period is investigated 5 times altogether later on.Investigation record standard is according to carrying out state of an illness classification by " insect pest classification of GB-T 23222-2008 tobacco disease and investigation method ", calculating the incidence of disease and disease index.Control efficiency is calculated according to following formula.
。
Data processing method: field data all adopts Excel and DPS software to put in order, analyze, and before the variance analysis sick index is changed (the 100-disease refers to) according to carrying out standardization, and numerical value is big more, and then the state of an illness is light more.
The production management measure: mid-July sowing, late August thinning, final singling, leaf-cutting by the end of August the 1st time sprays the 1st time before cutting seedling, sprays totally three times 1 time before cutting seedling at every turn.During transplanting, reject the cigarette strain that the TMV classical symptom is arranged, plant and carried out intertill and clean tillage in back 33 days, plant cigarette strain in back 40 days and get into prosperous long-term.
Each handles the control efficiency (unit: %) that contrasts with clear water table 1. processed group
| Processing mode | Plant back 12d | Plant back 19d | Plant back 26d | 1 plants back 33d | Plant back 40d |
| Processed group 1 | 98.16 | 72.18 | 63.91 | 58.95 | 47.21 |
| Processed group 2 | 95.18 | 78.19 | 71.66 | 69.53 | 52.29 |
| Processed group 3 | 69.81 | 56.21 | 50.78 | 48.23 | 38.91 |
| Processed group 4 | 71.14 | 59.65 | 41.47 | 39.17 | 31.62 |
| Processed group 5 | 74.88 | 63.69 | 54.37 | 48.29 | 38.12 |
| Processed group 6 | 81.13 | 69.64 | 51.98 | 49.82 | 31.65 |
| Control group 7 (disease refers to) | 11.02 | 33.66 | 34.57 | 57.02 | 67.12 |
Annotate: "+" expression increases in the table, and "-" expression reduces.Down together.
Table 2. is planted the significance of difference analysis (LSD method) of the anti-TMV of back each processed group cigarette strain of 40d
| Processing mode | Average | 5% significance level | 1% utmost point significance level |
| Processed group 1 | 70.61 | a | A |
| Processed group 2 | 69.78 | a | AB |
| Processed group 3 | 56.69 | Ab | ABC |
| Processed group 4 | 60.27 | Ab | ABC |
| Processed group 5 | 62.41 | Ab | ABC |
| Processed group 6 | 66.35 | Ab | ABC |
| Control group 7 | 32.88 | " bcde " | " BCDE " |
Claims (9)
1. prevent and treat the method that tobacco floating seed rearing field planting phase tobacco mosaic takes place for one kind, it is characterized in that comprising transplant before cigarette seedling diseases viral disease detect, transplanted seedling is handled, planting site is handled and transplant the back management, be specially:
A, in the cigarette transplantation of seedlings preceding 7 days carry the TMV situation to the cigarette seedling and detect, and surpass in 5% the seedling-cultivating tray cigarette seedling with malicious rate and give it up;
B, transplanted seedling handle: before the cigarette transplantation of seedlings, gave organic antivirotic 1 time that the cigarette seedling sprays (volume ratio) 0.5 ~ 5% in 1 ~ 2 day, the amount of spraying is per hundred square metres of seedling-cultivating tray 60 ~ 120Kg;
C, planting site are handled: before the cigarette transplantation of seedlings, to applying organic antivirotic in the planting site cigarette pool, formulation rate is 0.5 ~ 1.0 Kg/ mu;
D, cigarette transplantation of seedlings: the cigarette seedling is sorted, reject weak seedling, carry out the cigarette transplantation of seedlings by technical specification then;
E, transplant the back management: gave organic antivirotic 1 time that the cigarette seedling sprays (volume ratio) 0.5 ~ 5% after the cigarette transplantation of seedlings in per 6 ~ 7 days, the amount of spraying is 15 ~ 40Kg/ mu, sprays continuously 3 ~ 4 times.
2. the method that control tobacco floating seed rearing field planting phase tobacco mosaic according to claim 1 takes place; It is characterized in that: the method for the described detection of A step TMV is to extract 1 ~ 2% cigarette seedling of cigarette seedling sum by five-spot, adopts enzyme-linked immunosorbent assay that the cigarette seedling is carried detection TMV situation and detects.
3. the method that control tobacco floating seed rearing field planting phase tobacco mosaic according to claim 1 takes place; It is characterized in that: D, the described transplanting of E step and organic antivirotic spray in the process; Adversary and instrument disinfection, employed thimerosal are skim milk and the Tween 20 of (weight ratio) 0.1 ~ 1% or the clorox of (weight ratio) 0.1 ~ 1.0% of (weight ratio) 5 ~ 20%.
4. the method that control tobacco floating seed rearing field planting phase tobacco mosaic according to claim 1 takes place, it is characterized in that: described organic antivirotic is the organic inductive antivirotic.
5. the method that control tobacco floating seed rearing field planting phase tobacco mosaic according to claim 1 takes place, it is characterized in that: the described organic antivirotic of B step is AHO or chitosan.
6. the method that control tobacco floating seed rearing field planting phase tobacco mosaic according to claim 1 takes place, it is characterized in that: the described organic antivirotic of C step is polypeptide treasured or AHO.
7. the method that control tobacco floating seed rearing field planting phase tobacco mosaic according to claim 1 takes place, it is characterized in that: the described organic antivirotic of E step is AHO, chitosan, saikoside A or ginkolide B.
8. according to the method for claim 5 or 7 described control tobacco floating seed rearing field planting phase tobacco mosaics generations, it is characterized in that: the molecular weight of described chitosan is 500 ~ 1500.
9. the method that takes place according to claim 1,3,4,5,6 or 7 described control tobacco floating seed rearing field planting phase tobacco mosaics, it is characterized in that: said organic antivirotic is aqua, emulsion, granule, wetting powder, water dispersible granules.
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| CN105746321A (en) * | 2016-03-22 | 2016-07-13 | 中国烟草总公司重庆市公司巫溪分公司 | Tobacco floating seedling raising method capable of reducing disease spread of seedbed |
| CN108617713A (en) * | 2018-07-18 | 2018-10-09 | 合肥百绿盛农业科技有限公司 | A kind of preparation method of nuisanceless composite plant growth regulator |
| CN108739889A (en) * | 2018-07-18 | 2018-11-06 | 合肥百绿盛农业科技有限公司 | A kind of sweet osmanthus nursery stock plant growth regulator |
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| CN108782623A (en) * | 2018-07-18 | 2018-11-13 | 合肥百绿盛农业科技有限公司 | Efficient Yield increasing type plant growth regulator and its application |
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| CN110537470A (en) * | 2019-05-22 | 2019-12-06 | 贵州省烟草公司黔南州公司 | comprehensive prevention and control technology for tobacco rootstock diseases |
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| CN110089320A (en) * | 2019-04-11 | 2019-08-06 | 饶超奇 | A kind of application method and its application of chitosan solution |
| CN110537470A (en) * | 2019-05-22 | 2019-12-06 | 贵州省烟草公司黔南州公司 | comprehensive prevention and control technology for tobacco rootstock diseases |
| CN116195602A (en) * | 2022-12-05 | 2023-06-02 | 湖南省烟草公司永州市公司 | Composite medicament for preventing and treating tobacco common mosaic disease as well as preparation method and application thereof |
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