CN102676383A - Device for quickly detecting pathogen of infectious diseases on site and method thereof - Google Patents
Device for quickly detecting pathogen of infectious diseases on site and method thereof Download PDFInfo
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- CN102676383A CN102676383A CN201210191160XA CN201210191160A CN102676383A CN 102676383 A CN102676383 A CN 102676383A CN 201210191160X A CN201210191160X A CN 201210191160XA CN 201210191160 A CN201210191160 A CN 201210191160A CN 102676383 A CN102676383 A CN 102676383A
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- nucleic acid
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- bin
- conduit
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Abstract
The invention discloses a device for quickly detecting pathogen of infectious diseases on site and a method thereof, and relates to a medical detection device. The device consists of a nucleic acid extracting bin (1), a funnel-shaped conduit (2), a nucleic acid amplification reaction bin (3), a cylindrical conduit (4) and a product development bin (5) which are connected from top to bottom in sequence. The method comprises the following steps: 1, adding a sample solution of pathogen of infectious diseases into the nucleic acid extracting bin (1), and extracting nucleic acid by using a sediment boiling method; 2, allowing the nucleic acid to enter the nucleic acid amplification reaction bin (3) through the funnel-shaped conduit (2), and amplifying the nucleic acid through constant temperature water bath to obtain a nucleic acid product; and 3, allowing the nucleic acid product to enter the product development bin (5) through the cylindrical conduit (4), performing nucleic acid dye reaction, developing, and judging a final result according to the visual color change. The device can quickly detects the pathogen of infectious diseases on site, is simple, portable, easy to operate and low in cost, and does not require special instruments or reagents.
Description
Technical field
The present invention relates to a kind of medical science proofing unit, relate in particular to a kind of devices and methods therefor of field quick detection infectious disease pathogens.
Background technology
(Polymerase Chain Reaction, invention PCR) has epoch making significance, makes that people can be at the specific nucleic acid fragment of external on purpose unlimited amplification in the polymerase chain reaction.Its principle is similar in the body of DNA and duplicates, and just in centrifuge tube, gives external synthetic a kind of conditions suitable that provides of DNA.Through years of development, this technology is very ripe, other a lot of correlation technique of having derived simultaneously: like fluorescent quantitative PCR technique etc., has been most important and the most frequently used technology in molecular biology research and the clinical diagnosis field now; Wherein the appearance of PCR appearance then makes this technology realize robotization; Make that the technical application of PCR is more extensive; For example the diagnosing hereditary illness, detect pathogenic agent in the clinical samples nucleic acid, legal medical expert's sample made genetics identify, and the aspects of analyzing in the activated oncogene such as sudden change situation have all obtained using widely.
Because long, the amplification procedure of existing whole proliferation time is more complicated also, the result is not easy repetition, instrument costliness and be inconvenient to carry; Break out like certain transmissible disease and can not diagnose the related diseases substance timely and effectively; Delayed the time, during epidemic situation possibly cause situation out of control with uncontrollable; Cause unnecessary loss, so the existing employed instrument and equipment of nucleic acid amplification technologies are restricted in the context of detection of the sudden transmissible disease of New Development.
So developing a kind of simple and easy, portable, easy-operating proofing unit is very urgent and necessary in that New Development burst infectious disease pathogens are detected; For the treatment and the control epidemic situation of the sudden transmissible disease of New Development tries to gain time precious to one, this will be following main developing direction.
Summary of the invention:
The object of the invention just is to overcome the shortcoming and defect that prior art exists, and a kind of devices and methods therefor of field quick detection infectious disease pathogens simple and easy, portable, simple to operate is provided.
For realizing above-mentioned purpose, the present invention adopts following technical scheme:
One, the device of field quick detection infectious disease pathogens (abbreviation device)
This device is made up of the nucleic acid extraction storehouse 1 that connects successively up and down, funnel-form conduit 2, nucleic acid amplification reaction storehouse 3, cylindric conduit 4 and product colour developing storehouse 5.
Two, the method for field quick detection infectious disease pathogens (abbreviation method)
Present method comprises the following steps:
1. in nucleic acid extraction storehouse 1, add the infectious disease pathogens sample solution, utilize the deposition boiling method to extract nucleic acid;
2. nucleic acid is got into nucleic acid amplification reaction storehouse 3 through funnel-form conduit 2,, make nucleic acid amplification, get nucleic acid product through water bath with thermostatic control;
3. nucleic acid product is got into product colour developing storehouse 5 through cylindric conduit 4,, judge net result according to the colour-change of visual colour developing through nucleic acid dye reaction back colour developing.
The present invention has advantage and positively effect:
1. can realize the field quick detection infectious disease pathogens;
2. install simple and easy, portable, easy to operate;
3. with low cost, need not specific apparatus and reagent.
Description of drawings:
Fig. 1 is the structural representation of this device;
Among the figure:
1-nucleic acid extraction storehouse;
2-funnel-form conduit;
3-nucleic acid amplification reaction storehouse;
The cylindric conduit of 4-;
5-product colour developing storehouse.
Embodiment
One, device
1, overall
Like Fig. 1, this device is made up of the nucleic acid extraction storehouse 1 that connects successively up and down, funnel-form conduit 2, nucleic acid amplification reaction storehouse 3, cylindric conduit 4 and product colour developing storehouse 5.
2, functional component
1) the nucleic acid extraction storehouse 1
Nucleic acid extraction storehouse 1 is a kind of container that is provided with upper and lower opening.
2) the funnel-form conduit 2
Funnel-form conduit 2 is a kind of general parts.
3) the nucleic acid amplification reaction storehouse 3
Nucleic acid amplification reaction storehouse 3 is a kind of containers that are provided with upper and lower opening.
4) cylindric conduit 4
5) product colour developing storehouse 5
Product colour developing storehouse 5 is a kind of containers that are provided with upper shed.
3, method of use
Utilize this device rapid detection infectious disease pathogens:
During detection, the 40 microlitre infectious disease pathogens samples of getting are joined in the nucleic acid extraction storehouse 1, add 10 microlitre lysis buffers again, deposition is boiled after 10 minutes and is extracted nucleic acid;
Nucleic acid is imported to nucleic acid amplification reaction storehouse 3 through funnel-form conduit 2, place in the water-bath (being the adjustable equipment of a kind of temperature, is liquid in it) to react with the previously prepd reaction solution, 65 ℃, 60min gets nucleic acid product;
Nucleic acid product is imported product colour developing storehouse 5 through cylindric conduit 4, develop the color through dyestuff and accomplish detection;
Colour-change according to visual colour developing is judged net result.
Claims (2)
1. the device of field quick detection infectious disease pathogens is characterized in that:
Form by the nucleic acid extraction storehouse (1) that connects successively up and down, funnel-form conduit (2), nucleic acid amplification reaction storehouse (3), cylindric conduit (4) and product colour developing storehouse (5).
2. based on the Device Testing method of the described a kind of field quick detection infectious disease pathogens of claim 1, it is characterized in that comprising the following steps:
1. in nucleic acid extraction storehouse (1), add the infectious disease pathogens sample solution, utilize the deposition boiling method to extract nucleic acid;
2. nucleic acid is got into nucleic acid amplification reaction storehouse (3) through funnel-form conduit (2),, make nucleic acid amplification, get nucleic acid product through water bath with thermostatic control;
3. nucleic acid product is got into product colour developing storehouse (5) through cylindric conduit (4),, judge net result according to the colour-change of visual colour developing through nucleic acid dye reaction back colour developing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210191160XA CN102676383B (en) | 2012-06-11 | 2012-06-11 | Device for quickly detecting pathogen of infectious diseases on site and method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210191160XA CN102676383B (en) | 2012-06-11 | 2012-06-11 | Device for quickly detecting pathogen of infectious diseases on site and method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102676383A true CN102676383A (en) | 2012-09-19 |
| CN102676383B CN102676383B (en) | 2013-11-20 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210191160XA Expired - Fee Related CN102676383B (en) | 2012-06-11 | 2012-06-11 | Device for quickly detecting pathogen of infectious diseases on site and method thereof |
Country Status (1)
| Country | Link |
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| CN (1) | CN102676383B (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008002882A2 (en) * | 2006-06-26 | 2008-01-03 | Blood Cell Storage, Inc. | Device and method for extraction and analysis of nucleic acids from biological samples |
| WO2009002511A1 (en) * | 2007-06-26 | 2008-12-31 | Applera Corporation | Nucleic acid sample preparation by exclusion of dna |
| CN202658156U (en) * | 2012-06-11 | 2013-01-09 | 武汉大学 | Device for quickly detecting infectious pathogen on site |
-
2012
- 2012-06-11 CN CN201210191160XA patent/CN102676383B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008002882A2 (en) * | 2006-06-26 | 2008-01-03 | Blood Cell Storage, Inc. | Device and method for extraction and analysis of nucleic acids from biological samples |
| WO2009002511A1 (en) * | 2007-06-26 | 2008-12-31 | Applera Corporation | Nucleic acid sample preparation by exclusion of dna |
| CN202658156U (en) * | 2012-06-11 | 2013-01-09 | 武汉大学 | Device for quickly detecting infectious pathogen on site |
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| Publication number | Publication date |
|---|---|
| CN102676383B (en) | 2013-11-20 |
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| CB03 | Change of inventor or designer information |
Inventor after: Liu Yingle Inventor after: Shi Kang Inventor after: Xiang Rong Inventor before: Xiang Rong |
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Free format text: CORRECT: INVENTOR; FROM: XIANG RONG TO: LIU YINGLE SHI KANG XIANG RONG |
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