CN102675478A - Method for extracting and purifying rhodiola rosea polysaccharides - Google Patents
Method for extracting and purifying rhodiola rosea polysaccharides Download PDFInfo
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- CN102675478A CN102675478A CN2011100612413A CN201110061241A CN102675478A CN 102675478 A CN102675478 A CN 102675478A CN 2011100612413 A CN2011100612413 A CN 2011100612413A CN 201110061241 A CN201110061241 A CN 201110061241A CN 102675478 A CN102675478 A CN 102675478A
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- radix rhodiolae
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- rhodiolae polysaccharide
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Abstract
The invention discloses a method for extracting and purifying rhodiola rosea polysaccharides. The preparation method comprises the following steps of: adding a rhodiola rosea raw material which is degreased and pretreated with an ethanol solution into a water bath for performing reflux extraction; concentrating an extracting solution; performing alcohol precipitation to obtain a crude rhodiola rosea polysaccharide extract; and decolorizing and purifying with macroporous adsorption resin to obtain a rhodiola rosea polysaccharide extract. Due to the adoption of the method, impurities such as most pigments and the like existing in the rhodiola rosea polysaccharides can be removed on the premise of not damaging the structures and activity of the rhodiola rosea polysaccharides, and the purity of the rhodiola rosea polysaccharides is increased.
Description
Technical field
The present invention relates to a kind of is the method that raw material extracts the purifying Radix Rhodiolae polysaccharide with the Root of Kirilow Rhodiola.
Background technology
Radix Rhodiolae polysaccharide be from Root of Kirilow Rhodiola, extract and a kind of water-soluble polysaccharide.There are some researches show; Radix Rhodiolae polysaccharide is the main active ingredient that Root of Kirilow Rhodiola is regulated immunity, delayed senility; Can improve symptoms such as the elderly's fatiguability, poor appetite and blurred vision, have strengthening immunity, anticancer, reducing blood-fat, AFL, enhancing hemopoietic function, anti-ageing, prevent genetic damage, suppress effects such as tumor growth and cell mutation.Have multiple pharmacological effect and physiological function in view of Radix Rhodiolae polysaccharide, can be widely used in medicine, foodstuffs industry, the research of its extracting and purifying method is seemed particularly important.The Radix Rhodiolae polysaccharide extract of selling on the market often too much causes color darker because of pigment impurity, though some Radix Rhodiolae polysaccharide extract lighter color is decoloured because of the oxidation style that adopt more, destroys big to the structure and the activity of Radix Rhodiolae polysaccharide.
Summary of the invention
In order to address the above problem, to the object of the present invention is to provide and a kind ofly can improve Radix Rhodiolae polysaccharide purity in the Radix Rhodiolae polysaccharide extract, the method for effectively sloughing pigment.
In order to achieve the above object; Technical scheme of the present invention is: a kind ofly can improve Radix Rhodiolae polysaccharide purity in the Radix Rhodiolae polysaccharide extract; Effectively slough the method for pigment, it comprises adding water and carry out the water-bath refluxing extraction through degreasing, the pretreated Root of Kirilow Rhodiola raw material of ethanolic soln, then extracting solution is concentrated; Alcohol precipitation gets the Radix Rhodiolae polysaccharide crude extract, again with the macroporous adsorbent resin purifying that decolours.
The described water that adds carries out the water-bath refluxing extraction, and the quality of added water is 4～6 times through pretreated Root of Kirilow Rhodiola raw material.
Described to add the number of times that water carries out the water-bath refluxing extraction be 1～4 time, and extraction time is each 1～3 hour, after having extracted at every turn with the extracting solution filtered while hot.
Described pretreated Root of Kirilow Rhodiola raw material can be made by following method: is 1: 5 with sherwood oil (60～90 ℃) by mass volume ratio with the sub-medicinal material of Root of Kirilow Rhodiola, and the water-bath refluxing extraction is 0.5～2 hour under 70～90 ℃ of constant temperature, repeats 2～4 times and carries out degreasing; With 60～90% (V/V) ethanol, is 1: 5 by mass volume ratio with the dried material after the degreasing, and the water-bath refluxing extraction is 1～3 hour under 70～90 ℃ of constant temperature, repeats 2～6 times, filters, and filtrating is abandoned it, and drying under reduced pressure volatilizes the surperficial solvent of raw material.
Described that extracting solution is concentrated; Alcohol precipitation gets the Radix Rhodiolae polysaccharide crude extract and comprises the following steps: extracting solution is evaporated to 1/3 of original volume; The cooling back adds the absolute ethyl alcohol of 4～8 times of volumes; 4～8 ℃ of hold over night are centrifugal 10～30 minutes of 3000～5000r/min with rotating speed, get deposition and promptly get Radix Rhodiolae polysaccharide and slightly get thing.
Describedly comprise the following steps: Radix Rhodiolae polysaccharide is slightly got the aqueous solution that thing is mixed with 5～15g/l with macroporous resin decolouring purifying; Slightly get thing and SP700 macroporous adsorbent resin mass ratio in 0.1: 1～0.3: 1 ratio mixing with Radix Rhodiolae polysaccharide, place that 4～8 hours after-filtration of jolting get polysaccharide solution on the reciprocal shaker; With the water flushing macroporous resin of 3～6 times of dry weights of SP700 macroporous adsorbent resin, filter again.Merging filtrate and polysaccharide solution concentrate drying under reduced pressure.
Radix Rhodiolae polysaccharide process for extracting provided by the invention is carrying out degreasing through sherwood oil, and the pretreated Root of Kirilow Rhodiola raw material of ethanolic soln extracts Radix Rhodiolae polysaccharide with water extraction and alcohol precipitation method, and utilizes the SP700 macroporous adsorbent resin with the Radix Rhodiolae polysaccharide purifying that decolours.This method can be removed the most of pigment that exists in the Radix Rhodiolae polysaccharide under the structure of not damaging Radix Rhodiolae polysaccharide and active prerequisite, improve the purity of Radix Rhodiolae polysaccharide.
Embodiment
The method that with the Root of Kirilow Rhodiola is raw material extracts the purifying Radix Rhodiolae polysaccharide provided by the invention is to carry out according to the following step order: the Root of Kirilow Rhodiola that will remove impurity is pulverized; Is 1: 5 with sherwood oil (60～90 ℃) by mass volume ratio; The water-bath refluxing extraction is 1 hour under 85 ℃ of constant temperature, repeats 2 times and carries out degreasing; With 80% (V/V) ethanol, is 1: 5 by mass volume ratio with the dried material after the degreasing, and the water-bath refluxing extraction is 2 hours under 90 ℃ of constant temperature, repeats 4 times, filters, and filtrating is abandoned it, drying under reduced pressure, and the solvent that volatilizes the raw material surface obtains through pretreated Root of Kirilow Rhodiola raw material; At the above-mentioned water that in pretreated Root of Kirilow Rhodiola raw material, adds 5 times of quality, water-bath refluxing extraction 2 times, each 2 hours and filtered while hot extracting solution; : extracting solution is evaporated to 1/3 of original volume, and cooling back adds the absolute ethyl alcohol of 6 times of volumes, and 6 ℃ of hold over night are centrifugal 20 minutes of 4000r/min with the rotating speed, gets deposition and promptly gets Radix Rhodiolae polysaccharide and slightly get thing; Radix Rhodiolae polysaccharide is slightly got the aqueous solution that thing is mixed with 10g/l, slightly get thing and SP700 macroporous adsorbent resin mass ratio in 0.18: 1 ratio mixing, place that 6 hours after-filtration of jolting get polysaccharide solution on the reciprocal shaker with Radix Rhodiolae polysaccharide; With the water flushing macroporous resin of 5 times of dry weights of SP700 macroporous adsorbent resin, filter again.Merging filtrate and polysaccharide solution concentrate, and drying under reduced pressure gets the Radix Rhodiolae polysaccharide extract.
Claims (7)
1. one kind can be improved Radix Rhodiolae polysaccharide purity in the Radix Rhodiolae polysaccharide extract; Effectively slough the method for pigment; It comprises adding water and carry out the water-bath refluxing extraction through degreasing, the pretreated Root of Kirilow Rhodiola raw material of ethanolic soln; Then extracting solution is concentrated, alcohol precipitation gets the Radix Rhodiolae polysaccharide crude extract, again with the macroporous adsorbent resin purifying that decolours.
2. preparation method as claimed in claim 1 is characterized in that the described water that adds carries out the water-bath refluxing extraction, and added quality is 4～6 times through pretreated Root of Kirilow Rhodiola raw material.
3. preparation method as claimed in claim 1 is characterized in that described to add the number of times that water carries out the water-bath refluxing extraction be 1～4 time, and extraction time is each 1～3 hour, after having extracted at every turn with the extracting solution filtered while hot.
4. preparation method as claimed in claim 1; It is characterized in that described pretreated Root of Kirilow Rhodiola raw material can be made by following method: is 1: 5 with sherwood oil (60～90 ℃) by mass volume ratio with the sub-medicinal material of Root of Kirilow Rhodiola; The water-bath refluxing extraction is 0.5～2 hour under 70～90 ℃ of constant temperature, repeats 2～4 times and carries out degreasing; With 60～90% (V/V) ethanol, is 1: 5 by mass volume ratio with the dried material after the degreasing, and the water-bath refluxing extraction is 1～3 hour under 70～90 ℃ of constant temperature, repeats 2～6 times, filters, and filtrating is abandoned it, and drying under reduced pressure volatilizes the surperficial solvent of raw material.
5. preparation method as claimed in claim 1; It is characterized in that described that extracting solution is concentrated; Alcohol precipitation gets the Radix Rhodiolae polysaccharide crude extract and comprises the following steps: extracting solution is evaporated to 1/3 of original volume, and the cooling back adds the absolute ethyl alcohol of 4～8 times of volumes, 4～8 ℃ of hold over night; With rotating speed is centrifugal 10～30 minutes of 3000～5000r/min, gets deposition and promptly gets Radix Rhodiolae polysaccharide and slightly get thing.
6. preparation method as claimed in claim 1; It is characterized in that describedly comprising the following steps: Radix Rhodiolae polysaccharide is slightly got the aqueous solution that thing is mixed with 5～15g/l with macroporous resin decolouring purifying; Slightly get thing and SP700 macroporous adsorbent resin mass ratio in 0.1: 1～0.3: 1 ratio mixing with Radix Rhodiolae polysaccharide, place that 4～8 hours after-filtration of jolting get polysaccharide solution on the reciprocal shaker; With the water flushing macroporous resin of 3～6 times of dry weights of SP700 macroporous adsorbent resin, filter again.Merging filtrate and polysaccharide solution concentrate drying under reduced pressure.
7. the Radix Rhodiolae polysaccharide extract that makes like each described preparation method of claim 1～6.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011100612413A CN102675478A (en) | 2011-03-14 | 2011-03-14 | Method for extracting and purifying rhodiola rosea polysaccharides |
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| CN2011100612413A CN102675478A (en) | 2011-03-14 | 2011-03-14 | Method for extracting and purifying rhodiola rosea polysaccharides |
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| CN102675478A true CN102675478A (en) | 2012-09-19 |
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| CN2011100612413A Pending CN102675478A (en) | 2011-03-14 | 2011-03-14 | Method for extracting and purifying rhodiola rosea polysaccharides |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106519056A (en) * | 2016-11-17 | 2017-03-22 | 青海泰柏特生物科技有限公司 | Method for extracting and separating water-soluble polysaccharide from quinoa bran |
| CN108967900A (en) * | 2018-07-24 | 2018-12-11 | 安徽顶康食品有限公司 | A kind of selenium-rich salubrity powder and preparation method thereof |
-
2011
- 2011-03-14 CN CN2011100612413A patent/CN102675478A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106519056A (en) * | 2016-11-17 | 2017-03-22 | 青海泰柏特生物科技有限公司 | Method for extracting and separating water-soluble polysaccharide from quinoa bran |
| CN108967900A (en) * | 2018-07-24 | 2018-12-11 | 安徽顶康食品有限公司 | A kind of selenium-rich salubrity powder and preparation method thereof |
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Application publication date: 20120919 |