CN102669185A - Water release method of culex larva bio-control bacteria - Google Patents
Water release method of culex larva bio-control bacteria Download PDFInfo
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- CN102669185A CN102669185A CN2012100828925A CN201210082892A CN102669185A CN 102669185 A CN102669185 A CN 102669185A CN 2012100828925 A CN2012100828925 A CN 2012100828925A CN 201210082892 A CN201210082892 A CN 201210082892A CN 102669185 A CN102669185 A CN 102669185A
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- culex
- mesh bag
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- water body
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Abstract
The invention discloses a water release method of culex larva bio-control bacteria. In the method, infected culex larvas are added into a fine-hole mesh bag, and the fine-hole mesh bag filled with the infected culex larvas is arranged in culex larva water. Compared with the bio-control bacteria directly released in the water, the method is high in germination percentage and better in bio-control effect.
Description
Technical field
The invention belongs to biotechnology, the concrete water body method for releasing that relates to a kind of culex larva biocontrol microorganisms.
Background technology
Culex mainly multiplies near the polluted-water human settlements, and the rich water cylinder in the sewage pool in the city, sewage ditch, catch-basin ponding, sewer ponding, garden and orchard, the hollow ponding in construction site all are its main breeding site.Culex also is one of most important sanitary insect pest, through movable harassing and wrecking people's life, can prevent and treat significant through biting the communicable diseases such as propagating bancroftosis and Japanese Type-B encephalitis of sucking blood on the other hand on the one hand.Traditional mosquito control is main with chemical control mainly, yet because the long-term use of a large amount of chemical insecticides, the pesticide resistance of mosquito is also serious day by day; Force people to use more the insecticide of high dose to kill mosquito; Cause mosquito to chemical insecticide produce more by force, the resistance of wide spectrum more, form vicious circle, serious environment pollution; Destroy the ecological balance, personnel's poisoning also thread has generation.Therefore, how to develop mosquito efficiently, to the environmentally friendly insecticide of human body safety, become the current important problem in science that need to solve, biological control has received increasing attention.
The biological control of mosquito mainly comprises biocontrol microorganisms and predacious huge mosquito, cyclops and the fish of parasitism, and biocontrol microorganisms is more convenient owing to cultivating and storing, and has received more attention.Yet; In the research of mosquito biocontrol microorganisms, the effect in the laboratory is all good, but the performance in using at the scene is poor; Main and method for releasing has relation; Traditional method for releasing mainly is in the target water body, to spray thalline or spore, but its viability in new environment is relatively poor, thereby is difficult to reach due effect.
Summary of the invention
The object of the present invention is to provide a kind of method for releasing to culex larva biocontrol microorganisms; This method fixes a point to discharge the culex larva by parasitic in water body; Make polypide go up parasitic biocontrol microorganisms for some time laundering period in new water body, thereby improve the viability of thalline, improve control efficiency.
For realizing above purpose, the present invention proposes following technical scheme:
A kind of water body method for releasing of culex larva biocontrol microorganisms, its step is following:
(1) the biocontrol microorganisms spore is pressed 10
6The concentration of individual/ml inserts in the Breeding container of normal culex larva, meets bacterium 12h;
(2) choose the gauze making mesh bag that mesh diameter is about 1 mm;
(3) will connect bacterium, the metainfective culex larva mesh bag in (2) of packing into tightens up network interface;
(4) fixed point of the mesh bag in (3) is put into the target water body of control, water body did not have the mesh bag mouth;
(5) mesh bag mouth in (4) is tightened up, be fixed in by the water body.
The present invention is a release vehicle with the mesh bag that metainfective culex larva is housed; This method fixes a point to discharge the culex larva by parasitic in water body; Make polypide go up parasitic biocontrol microorganisms for some time laundering period in new water body, thereby improve the viability of thalline, improve control efficiency.Compare with in water body, directly discharging biocontrol microorganisms, this method germination rate is high, and biocontrol effect is better.
Description of drawings
The different method for releasing of Fig. 1 kill the influence of mosquito children effect to beauveria bassiana Beauveria bassiana.
The different method for releasing of Fig. 2 kill the influence of mosquito children effect to Metarhizium anisopliae Metarhizium anisopliae.
The different method for releasing of Fig. 3 kill the influence of mosquito children effect to paecilomyces lilacinus Paecilomyces lilacinus.
Embodiment
Step of the present invention is following:
(1) biocontrol microorganisms is inserted in the Breeding container of normal culex larva by optimal proportion, connect bacterium;
(2) choose the gauze making mesh bag that mesh diameter is about 1mm, the about 5mm of mesh bag bore, the about 10cm of mesh bag length, the mesh bag mouth has length-adjustable bungee;
(3) will connect bacterium, the metainfective culex larva mesh bag in (2) of packing into, tension network interface;
(4) fixed point of the mesh bag in (3) is put into the target water body of control, do not have mesh bag mouth 10cm to be advisable with water body;
(5) with the stay cord tension of mesh bag mouth in (4), be fixed in by the water body;
The method for releasing of the culex larva biocontrol microorganisms that the present invention adopted can improve release efficiency, reduces the death of biocontrol microorganisms, thereby improves control efficiency.
Following instance is to further specify of the present invention, rather than restriction.
Instance:
Respectively with 5 d behind inoculation beauveria bassiana Beauveria bassiana, Metarhizium anisopliae Metarhizium anisopliae and the paecilomyces lilacinus Paecilomyces lilacinus 3 age culex pipiens pallens larvae about 30 put into mesh bag after; Get outdoor sewage; Be divided into 6 parts; Insert the culex of laboratory rearing, every part has 50 of culex pipiens pallens larvaes approximately, and wherein 3 parts discharge to fill 50 mesh bags that connect the bacterium Culex pipiens pallens; 3 parts directly add the beauveria bassiana spore in addition, make spore concentration reach 10
6Individual/ml.Add up the metainfective death toll of larva every day, calculating adds up to unite lethality.The result shows that as far as three kinds of mosquito biocontrol microorganisms, the children of the mosquito extremely effect of mesh bag method for releasing all significantly is superior to directly discharging the method (seeing Fig. 1-3) of spore.
Claims (1)
1. the water body method for releasing of a culex larva biocontrol microorganisms, its step is following:
(1) the biocontrol microorganisms spore is pressed 10
6The concentration of individual/ml inserts in the Breeding container of normal culex larva, meets bacterium 12 h;
(2) choose the gauze making mesh bag that mesh diameter is about 1 mm;
(3) will connect bacterium, the metainfective culex larva mesh bag in (2) of packing into tightens up network interface;
(4) fixed point of the mesh bag in (3) is put into the young target water body of control mosquito, water body did not have the mesh bag mouth;
(5) mesh bag mouth in (4) is tightened up, be fixed in by the water body.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012100828925A CN102669185A (en) | 2012-03-27 | 2012-03-27 | Water release method of culex larva bio-control bacteria |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012100828925A CN102669185A (en) | 2012-03-27 | 2012-03-27 | Water release method of culex larva bio-control bacteria |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102669185A true CN102669185A (en) | 2012-09-19 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012100828925A Pending CN102669185A (en) | 2012-03-27 | 2012-03-27 | Water release method of culex larva bio-control bacteria |
Country Status (1)
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002065835A2 (en) * | 2001-02-16 | 2002-08-29 | Valent Biosciences Corp. | Mixture of bacillus thuringiensis subspecies israelensis and bac illus sphaericus for management of resistance to mosquito larvicides |
| CN101347129A (en) * | 2008-09-01 | 2009-01-21 | 佛山市正典生物技术有限公司 | Bacillus thuringiensis suspending agent for killing mosquito |
| CN101554179A (en) * | 2008-04-08 | 2009-10-14 | 武汉天惠生物工程有限公司 | Mosquito killing Bacillus sphaericus leafing agent and preparation method thereof |
| CN102154171A (en) * | 2011-01-13 | 2011-08-17 | 河北农业大学 | Bacillus thuringiensis capable of killing mosquito larvae effectively |
-
2012
- 2012-03-27 CN CN2012100828925A patent/CN102669185A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002065835A2 (en) * | 2001-02-16 | 2002-08-29 | Valent Biosciences Corp. | Mixture of bacillus thuringiensis subspecies israelensis and bac illus sphaericus for management of resistance to mosquito larvicides |
| CN101554179A (en) * | 2008-04-08 | 2009-10-14 | 武汉天惠生物工程有限公司 | Mosquito killing Bacillus sphaericus leafing agent and preparation method thereof |
| CN101347129A (en) * | 2008-09-01 | 2009-01-21 | 佛山市正典生物技术有限公司 | Bacillus thuringiensis suspending agent for killing mosquito |
| CN102154171A (en) * | 2011-01-13 | 2011-08-17 | 河北农业大学 | Bacillus thuringiensis capable of killing mosquito larvae effectively |
Non-Patent Citations (4)
| Title |
|---|
| ANDREW F.READ: "Mosquitoes Cut Short", 《SCIENCE》, vol. 323, 31 December 2009 (2009-12-31), pages 51 - 52 * |
| CONOR J. MCMENIMAN1 等: "Stable Introduction of a Life-Shortening Wolbachia Infection into the Mosquito Aedes aegypti", 《SCIENCE》, vol. 323, 31 December 2009 (2009-12-31), pages 141 - 144 * |
| LINGUANHUA: "用细菌感染蚊子防治登革热", 《三农直通车HTTP://WWW.GDCCT.GOV.CN/AGRITECH/KJRD/201109/T20110901_576684.HTML#TEXT》, 1 September 2011 (2011-09-01), pages 1 - 2 * |
| 张光学 等: "蚊虫幼虫生物防治研究进展", 《中华实验和临床感染病杂志(电子版)》, vol. 2, no. 2, 30 June 2008 (2008-06-30), pages 59 - 63 * |
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Application publication date: 20120919 |