CN102660460A - Method for screening non-photosynthetic high-efficiency carbon immobilization microorganism under aerobic conditions - Google Patents

Method for screening non-photosynthetic high-efficiency carbon immobilization microorganism under aerobic conditions Download PDF

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CN102660460A
CN102660460A CN2012101435143A CN201210143514A CN102660460A CN 102660460 A CN102660460 A CN 102660460A CN 2012101435143 A CN2012101435143 A CN 2012101435143A CN 201210143514 A CN201210143514 A CN 201210143514A CN 102660460 A CN102660460 A CN 102660460A
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substratum
content
sediment
electron donor
described step
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胡佳俊
王磊
张士萍
席雪飞
胡煜
单伊娜
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Tongji University
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Abstract

The invention relates to the field of microbial immobilization of CO2, in particular to a method for screening a non-photosynthetic high-efficiency carbon immobilization microorganism under aerobic conditions. The method comprises the following steps of: (1) preparing a culture medium for autotrophic microorganisms, and adding a solution of trace elements; (2) adding seawater or a marine sediment into the culture medium which is obtained in step (1) and contains the trace elements, and culturing the seawater or the marine sediment containing a mixed carbon immobilization strain by using hydrogen under aerobic conditions to obtain a bacterial solution of the mixed carbon immobilization strain; (3) preparing an electron donor concentrated solution; (4) adding the electron donor concentrated solution obtained in step (3) into a culture solution which is obtained in step (1) and contains the trace elements; and (5) adding the bacterial solution of the mixed carbon immobilization strain into a culture medium obtained in step (4), and culturing under aerobic conditions. By the method, the carbon immobilization efficiency of non-photosynthetic carbon immobilization microorganisms can be effectively improved; and the method also has the advantages of simple process, high operability and certain economic benefit.

Description

A kind of aerobic condition is the non-photosynthetic efficient carbon method of microorganism admittedly of screening down
Technical field
The invention belongs to mikrobe fixation of C O 2The field relates to the non-photosynthetic efficient carbon method of microorganism admittedly of screening.
Background technology
CO 2The climate warming that " Greenhouse effect " that cause are caused is the great environmental problem that the current whole world faces.Point out according to IPCC the 4th assessment report: at a nearest decade (1995 ~ 2004 years), CO 2(the annual 9.2 hundred million tons of CO that advance the speed of equivalent discharging 2Equivalent) than the drainage rate of previous decade (1970 ~ 1994 years) (annual 4.3 hundred million tons of CO 2Equivalent) much higher." the 12 planning proposal draft " of China shows that China is with CO 2Reduction of discharging has been placed on the considerable position.And on the Cancun meeting of holding for the year ends 2010, China promises to undertake the year two thousand twenty, unit gross domestic product (GDP) CO 2Discharging will be than decline 40%-45% in 2005.While CO 2Be again carbon resource the abundantest on the earth, can change it into resource and the energy.Therefore, CO 2Be fixed on environment, the energy, resource aspect all have great importance.Current, considering how to reduce discharging CO 2Prerequisite under, research CO 2Recovery with fixing, can effectively reduce free CO in the environment 2, can it be regenerated as resource again, therefore caused the extensive interest of countries in the world.
CO 2Fixedly mainly contain physics forensic chemistry method and biological process, and most of physics method and chemical method all must connect and biological process comes final fixation of C O 2Biological process fixation of C O 2Mainly be to rely on plant and autotrophic microorganism, the photosynthesis of plants outbalance is also more paid attention to by the people traditionally.But there are various environment on the earth; Particular surroundings and the occasion (like the capture occasion of arid barren desert soil and industrial gaseous waste) that can not grow plant; The advantage of the environmental compatibility that Institute of Micro-biology has has just displayed; Therefore substance flow and the energy from whole biosphere flows mikrobe fixation of C O 2Significant.
If generally acknowledge at present and have higher microbial host photosynthetic microorganism of carbon efficiencies admittedly and the hydrogen-oxygen bacterium in the chemosynthetic bacteria.Photosynthetic microorganisms such as algae need illumination because in culturing process, with and thermo-labile and high concentration CO 2Characteristic, limited its practical application.Though and the hydrogen-oxygen bacterium is comparatively wide in range without illumination and growth scope; But it must provide with high concentration hydrogen in process of growth as electron donor; Therefore the conventional environment condition is difficult to meet its growth requirement, and in practical application, also there is serious potential safety hazard in hydrogen supply gas simultaneously.Given this, excavate efficient carbon mikrobe admittedly, for realizing that the solid carbon of mikrobe under the conventional environment condition is (like edatope and absorption industrial discharge CO without illumination and hydrogen supply 2The macro-organism reactor drum in) significant.
There is critical role the ocean in the global carbon process, will absorb 2.0Gt (1Gt=10 its every year 9T) anthropogenic discharge's CO 2, the research of solid carbon mikrobe also is the focus that global association area expert pays close attention to always in the ocean.For this reason, we gather the water and soil sample in a plurality of marine sites (comprising global four ocean, the dozens of countries and regions) from the whole world, have obtained the solid carbon microorganism species without illumination and hydrogen supply of a plurality of series through separation screening.But these floras are only through for a long time domestication (several months even more than a year); Its solid carbon efficiencies just can reach a higher level; And owing to these floras are made up of a plurality of bacterial classifications; The culture presevation that mixing microorganisms especially mixes autotrophic microorganism is a difficulty very, mainly is that its solid carbon efficiencies of bacterial classification recovery back has decline in various degree, these feature limits the industrial application of non-photosynthetic carbon fixation mikrobe; Use the hydrogen cultivation to use mixed electronic donor cultured method more earlier if utilize; Then (about 8 days) are screened from seawater or oceanogenic sedimentation matter sample and are obtained non-efficiently photosynthetic carbon fixation mikrobe at short notice, thereby effectively address these problems, and help further strengthening non-photosynthetic carbon fixation microorganism species potential economic benefit and social benefit in practical application.
Summary of the invention
The objective of the invention is to provides the non-photosynthetic efficient carbon method of microorganism admittedly of screening under a kind of aerobic condition for the defective that overcomes prior art.
For realizing above-mentioned purpose, the technical scheme that the present invention adopted is:
A kind of aerobic condition is the non-photosynthetic efficient carbon method of microorganism admittedly of screening down, comprises following steps:
(1) substratum of preparation autotrophic microorganism; In above-mentioned substratum, add trace element solution then again;
(2) add seawater or oceanic sediment in the substratum that contains trace element that in step (1), obtains, the seawater of the solid carbon bacterial classification of the mixing that will contain or oceanic sediment use hydrogen to cultivate under aerobic condition, obtain mixing the bacterium liquid of solid carbon bacterial classification;
(3) preparation electron donor liquid concentrator;
(4) the electron donor liquid concentrator that makes in the step (3) is joined in the nutrient solution that contains trace element that makes in the step (1);
(5) obtain adding the bacterium liquid that step (2) is cultivated the solid carbon bacterial classification of mixing that obtains in the substratum to step (4), under aerobic condition, cultivate.
The substratum of autotrophic microorganism comprises following component in the described step (1): 0.5-1.0g/L KH 2PO 4, 1.0-2.0g/LK 2HPO 4, 0.1-0.2g/L MgSO 47H 2O, 10-30g/LNaCl, 0.001-0.01g/L CaCl 2, 0.0036mmol-0.036mmol/L ferrous ion and 0.0075-0.075mol/L NH 4 +Ion.
Described ferrous ion is from FeSO 47H 2O, FeSO 4Or FeCl 2
Described NH 4 +Ion is from (NH 4) 2SO 4Or NH 4Cl.
Described trace element solution comprises 1.68mg/LNa 2MoO 42H 2O, 0.4mg/LH 3BO 3, 1.0mg/LZnSO 47H 2O, 1.0mg/LMnSO 45H 2O, 7.0mg/LCuSO 45H 2O, 1.0mg/LCoCl 26H 2O or 1.0mg/LNiSO 47H 2Among the O more than one.
In the described step (1), add the 2ml trace element solution in every liter of nutrient solution.
The aerobic condition of described step (2) uses hydrogen to cultivate down: oxygen content is 5-20% in the gas; Oxygen content preferred 10%; Carbon dioxide content is greater than 0%, the preferred 5-30% of carbon dioxide content, and further the content of preferably carbon dioxide is 10%; Hydrogen content is 50-90%, hydrogen content preferred 80%; Incubation time is 4-8 days.
In the described step (2), oceanic sediment is the general name of the formed marine bottom sediment of marine sdeimentation, and the add-on of seawater is 25-75mL/L; The marine sediment consumption is 25-50mL/L in the marine sediment diluent; The marine sediment diluent is: the diluent of the 0.1g/L that marine sediment and sterilized water make.
Electron donor is selected from one or more in nitrite, thiosulphate and the sulfide in the described step (3).
Described nitrite is selected from NaNO 2Or KNO 2
Described thiosulphate is selected from Na 2S 2O 3Or K 2S 2O 3
Described sulfide is selected from Na 2S or K 2S.
In the described step (3), the concentration of every kind of electron donor is 80-200mg/mL in the electron donor liquid concentrator.
The consumption of mixed electronic donor is 1-15g/L nitrite, 1-15g/L thiosulphate or 1-15g/L sulfide in the described step (4).
Aerobic condition in the described step (5) is: oxygen content is 5-25% in the gas, and carbon dioxide content is greater than 0%, the preferred 5-30% of carbon dioxide content, and further the content of preferably carbon dioxide is 20%, incubation time is 4-8 days.
In the described step (5), add the bacterium liquid that 25-75mL step (2) obtains in the substratum that every 1L step (4) obtains.
Beneficial effect of the present invention is:
Having under the condition of hydrogen; Screening obtains non-photosynthetic carbon fixation microorganism from seawater or marine sediment earlier; Utilize the mixed electronic donor system that forms by nitrite, thiosulfate and sulfide again; Cultivate screening and obtain non-photosynthetic carbon fixation microorganism, can obtain the non-photosynthetic carbon fixation microorganism of higher solid carbon efficiencies at last; Our experiments show that, use the solid carbon efficiencies of the solid carbon microorganism that the inventive method obtained to exceed 210% and 100% respectively than the solid carbon microorganism that continuous use hydrogen or the screening of mixed electronic donor obtain.The solid carbon efficiencies of the solid carbon mikrobe that the inventive method obtained can reach 853mg CO 2/ L.Show that this method can screen the non-efficiently photosynthetic carbon fixation mikrobe of acquisition at short notice, can effectively be applied to fixation of C O 2Process in, thereby realize CO 2Resource utilization.It is simple, workable and have an advantage of certain economic benefit that the present invention also has technology.
Embodiment
Below in conjunction with specific embodiment, further set forth the present invention.
Embodiment 1
(1) substratum of autotrophic microorganism is cultivated in preparation
Culture medium prescription is (g/L) as follows: KH 2PO 4(1.0); K 2HPO 4(2.0); MgSO 47H 2O (0.2); NaCl (20) and CaCl 2(0.01); (NH 4) 2SO 4(0.038mol/L); FeSO 47H 2O (0.0036mmol/L); Go up whenever and to state the trace element solution that adds 2mL in the substratum again;
Trace element solution is selected from and comprises Na 2MoO 42H 2O (1.68); H 3BO 3(0.4); ZnSO 47H 2O (1.0); MnSO 45H 2O (1.0); CuSO 45H 2O (7.0); CoCl 26H 2O (1.0) and NiSO 47H 2O (1.0), concentration is in mg/L;
(2) obtain adding in the substratum from Australia, the coastal marine sediment diluent of Japan or Thailand to step (1); Add-on is that every 1L substratum adds 25mL marine sediment diluent (the marine sediment diluent is: the diluent of the 0.1g/L that marine sediment and sterilized water make.), under aerobic condition, use hydrogen to cultivate 4 days, obtain mixing the bacterium liquid of solid carbon bacterial classification;
Wherein, wherein mixed gas is made up of hydrogen, oxygen and carbonic acid gas, wherein hydrogen: oxygen: carbonic acid gas=80:10:10, volume ratio;
(3) respectively separately after the preparation as the NaNO of electron donor 2, Na 2S 2O 3And Na 2The liquid concentrator of S, its concentration is respectively 80mg/mL;
(4) according to 5.0g/L NaNO 2, 5.0g/L Na 2S 2O 3With 12.5g/L Na 2The consumption of S adds the electron donor liquid concentrator that step (3) obtains in the substratum that step (1) obtains;
(5) the bacterium liquid of step (2) being cultivated the solid carbon microbial strains that obtains after 4 days is inoculated in step (4) and obtains in the substratum; Inoculum size is that every 1L substratum adds the bacterium liquid that 25mL step (2) obtains; And under aerobic condition, cultivated 4 days; Wherein mixed gas is made up of air and carbonic acid gas, its air: carbonic acid gas=90:10, volume ratio.
Embodiment 2
(1) substratum of autotrophic microorganism is cultivated in preparation.Culture medium prescription is (g/L) as follows: KH 2PO 4(1.0); K 2HPO 4(2.0); MgSO 47H 2O (0.2); NaCl (20) and CaCl 2(0.01); (NH 4) 2SO 4(0.038mol/L); FeSO 47H 2O (0.0036mmol/L) goes up whenever and states the trace element solution that adds 2mL in the substratum again.
Trace element solution is selected from and comprises Na 2MoO 42H 2O (1.68); H 3BO 3(0.4); ZnSO 47H 2O (1.0); MnSO 45H 2O (1.0); CuSO 45H 2O (7.0); CoCl 26H 2O (1.0) and NiSO 47H 2O (1.0), concentration is in mg/L;
(2) obtain adding the seawater from the South Pole or marine site, the arctic in the substratum to step (1), add-on is that every 1L substratum adds 25mL seawater appearance, under aerobic condition, uses hydrogen to cultivate 4 days, obtains mixing the bacterium liquid of solid carbon bacterial classification; Wherein, wherein mixed gas is made up of hydrogen, oxygen and carbonic acid gas, wherein hydrogen: oxygen: carbonic acid gas=80:10:10, volume ratio.
(3) respectively separately after the preparation as the NaNO of electron donor 2, Na 2S 2O 3And Na 2The liquid concentrator of S, its concentration is respectively 160mg/mL;
(4) according to 4.6g/L NaNO 2, 5.0g/L Na 2S 2O 3With 12.0g/L Na 2The consumption of S adds the electron donor liquid concentrator that step (3) obtains in the substratum that step (1) obtains;
(5) the solid carbon microorganism species that step (2) cultivation was obtained after 4 days is inoculated in step (4) and obtains in the substratum; Inoculum size is that every 1L substratum adds the bacterium liquid that 25mL step (2) obtains; And under aerobic condition, cultivated 4 days, wherein, wherein mixed gas is made up of air and carbonic acid gas; Its air: carbonic acid gas=90:10, volume ratio.
Embodiment 3
(1) substratum of autotrophic microorganism is cultivated in preparation.Culture medium prescription is (g/L) as follows: KH 2PO 4(1.0); K 2HPO 4(2.0); MgSO 47H 2O (0.2); NaCl (20) and CaCl 2(0.01); (NH 4) 2SO 4(0.038mol/L); FeSO 4.7H 2O (0.0036mmol/L) goes up whenever and states the trace element solution that adds 2mL in the substratum again.
Trace element solution is selected from and comprises Na 2MoO 42H 2O (1.68); H 3BO 3(0.4); ZnSO 4.7H 2O (1.0); MnSO 45H 2O (1.0); CuSO 45H 2O (7.0); CoCl 26H 2O (1.0) and NiSO 47H 2O (1.0), concentration is in mg/L;
(2) obtain adding respectively in the substratum to step (1) and add Lay, Papua New Guinea, the South Pole and Arctic Sea water sample from Chinese Qingdao, Shanghai, Xiamen, Hainan, Australia, Phuket, THA, Japanese celestial platform, France; Add-on is that every 1L substratum adds 25mL seawater appearance; Under aerobic condition, use hydrogen to cultivate 4 days, obtain mixing the bacterium liquid of solid carbon bacterial classification; Wherein, wherein mixed gas is made up of hydrogen, oxygen and carbonic acid gas, wherein hydrogen: oxygen: carbonic acid gas=80:10:10, volume ratio;
(3) respectively separately after the preparation as the NaNO of electron donor 2, Na 2S 2O 3And Na 2The liquid concentrator of S, its concentration is respectively 160mg/mL;
(4) according to 4.6g/L NaNO 2, 5.0g/L Na 2S 2O 3With 12.0g/L Na 2The consumption of S adds the electron donor liquid concentrator that step (3) obtains in the substratum that step (1) obtains;
(5) step (2) is cultivated the solid carbon microorganism species that obtains after 4 days through centrifugal collection and mixing; Process and be inoculated in step (4) after the microbial cells liquid concentrator and obtain in the substratum, its inoculum size is that every 1L substratum adds 75mL bacterium liquid, and cultivation 4 days under aerobic condition; Wherein, Wherein mixed gas is made up of air and carbonic acid gas, its air: carbonic acid gas=90:10, volume ratio.
Among the embodiment; All under aerobic condition, use hydrogen to cultivate 4 days (hereinafter to be referred as first culture cycle) earlier; Under aerobic condition, cultivate 4 days (hereinafter to be referred as second culture cycle) more afterwards with the mixed electronic donor; For studying the solid carbon efficiencies of the non-photosynthetic carbon fixation mikrobe that is obtained in this screening process, the sample of second culture cycle of learning from else's experience is surveyed total organic carbon concentration in its nutrient solution; Because initial medium total organic carbon concentration is 0, and the utilizable carbon source of mikrobe has only CO in culturing process 2This inorganic carbon source is so the total organic carbon amount that has increased in the substratum all is come from mikrobe with CO 2Fixing gained.The result of embodiment 1 shows, screens the non-photosynthetic carbon fixation mikrobe from 3 marine sites respectively, its solid carbon efficiencies average out to 58g Cg -1Thalline d -1, the optimized electronic donor H that generally acknowledges is used in contrast 2The non-photosynthetic carbon fixation mikrobe that screening obtains is (in two culture cycle all with H 2Cultivate, and do not add the mixed electronic donor), and screen the non-photosynthetic carbon fixation mikrobe that obtains with the mixed electronic donor and (all cultivate with the mixed electronic donor in two culture cycle, and do not add H 2), effect of the present invention will exceed 230% and 130% respectively.Among the result of embodiment 2; Choose the seawater sample in the South Pole or marine site, the arctic; The non-photosynthetic carbon fixation mikrobe of using method of the present invention screening to obtain, its solid carbon efficiencies exceeds 210% and 100% respectively than the solid carbon mikrobe that continuous use hydrogen or the screening of mixed electronic donor obtain.Among the result of embodiment 3, the non-photosynthetic carbon fixation mikrobe that is obtained after second culture cycle is at the CO of 4 days internal fixing 2Amount has reached 853mg/L.The foregoing description shows, through method of the present invention, can obtain the non-photosynthetic carbon fixation mikrobe of higher solid carbon efficiencies at short notice.
To sum up visible; This method technology is simple; Workable; And, explain that this method obtains non-photosynthetic efficient carbon mikrobe admittedly for screening in the short period of time and has ubiquity for all effective from the seawater or the oceanogenic sedimentation matter sample in more than ten marine site, global 4 ocean, can effectively be used in mikrobe fixation of C O 2Process in, thereby realize CO 2Resource utilization.
The above-mentioned description to embodiment is can understand and use the present invention for ease of the those of ordinary skill of this technical field.The personnel of skilled obviously can easily make various modifications to these embodiment, and needn't pass through performing creative labour being applied in the General Principle of this explanation among other embodiment.Therefore, the invention is not restricted to the embodiment here, those skilled in the art are according to announcement of the present invention, and not breaking away from the improvement that category of the present invention makes and revise all should be within protection scope of the present invention.

Claims (10)

1. an aerobic condition screens non-photosynthetic efficient carbon method of microorganism admittedly down, it is characterized in that: comprise following steps:
(1) substratum of preparation autotrophic microorganism; In above-mentioned substratum, add trace element solution then again;
(2) obtain containing adding seawater or oceanic sediment in the micro-substratum to step (1), the seawater of the solid carbon bacterial classification of the mixing that will contain or oceanic sediment use hydrogen to cultivate under aerobic condition, obtain mixing the bacterium liquid of solid carbon bacterial classification;
(3) preparation electron donor liquid concentrator;
(4) the electron donor liquid concentrator that makes in the step (3) is joined in the nutrient solution that contains trace element that makes in the step (1);
(5) obtain adding the bacterium liquid that step (2) is cultivated the solid carbon bacterial classification of mixing that obtains in the substratum to step (4), under aerobic condition, cultivate.
2. method according to claim 1 is characterized in that: the substratum of autotrophic microorganism comprises following component in the described step (1): 0.5-1.0g/L KH 2PO 4, 1.0-2.0g/L K 2HPO 4, 0.1-0.2g/L MgSO 47H 2O, 10-30g/L NaCl, 0.001-0.01g/L CaCl 2, 0.0036mmol-0.036mmol/L ferrous ion and 0.0075-0.075mol/L NH 4 +Ion.
3. method according to claim 1 is characterized in that: described ferrous ion is from FeSO 4.7H 2O, FeSO 4Or FeCl 2
Or described NH 4 +Ion is from (NH 4) 2SO 4Or NH 4Cl.
4. method according to claim 1 is characterized in that: described trace element solution comprises 1.68mg/LNa 2MoO 42H 2O, 0.4mg/LH 3BO 3, 1.0mg/LZnSO 47H 2O, 1.0mg/LMnSO 45H 2O, 7.0mg/LCuSO 45H 2O, 1.0mg/LCoCl 26H 2O or 1.0mg/LNiSO 47H 2Among the O more than one.
5. method according to claim 1 is characterized in that: in the described step (1), add the 2ml trace element solution in every liter of nutrient solution.
6. method according to claim 1; It is characterized in that: the aerobic condition of described step (2) uses down the hydrogen cultivation to be: oxygen content is 5-20% in the gas, and oxygen content is preferred 10%, and carbon dioxide content is greater than 0%; The preferred 5-30% of carbon dioxide content; Further the content of preferably carbon dioxide is 10%, and hydrogen content is 50-90%, hydrogen content preferred 80%; Incubation time is 4-8 days.
7. method according to claim 1 is characterized in that: in the described step (2), oceanic sediment is the general name of the formed marine bottom sediment of marine sdeimentation, and the add-on of seawater is 25-75mL/L; The marine sediment consumption is 25-50mL/L in the marine sediment diluent; The marine sediment diluent is: the diluent of the 0.1g/L that marine sediment and sterilized water make.
8. method according to claim 1 is characterized in that: electron donor is selected from one or more in nitrite, thiosulphate and the sulfide in the described step (3).
9. method according to claim 8 is characterized in that: described nitrite is selected from NaNO 2Or KNO 2
Or described thiosulphate is selected from Na 2S 2O 3Or K 2S 2O 3
Or described sulfide is selected from Na 2S or K 2S.
10. method according to claim 1 is characterized in that: in the described step (3), the concentration of every kind of electron donor is 80-200mg/mL in the electron donor liquid concentrator;
Or the consumption of mixed electronic donor is 1-15g/L nitrite, 1-15g/L thiosulphate or 1-15g/L sulfide in the described step (4);
Or the aerobic condition in the described step (5) is: oxygen content is 5-25% in the gas, and carbon dioxide content is greater than 0%, the preferred 5-30% of carbon dioxide content, and further the content of preferably carbon dioxide is 20%, incubation time is 4-8 days;
Or in the described step (5), add the bacterium liquid that 25-75mL step (2) obtains in the substratum that every 1L step (4) obtains.
CN2012101435143A 2012-05-10 2012-05-10 Method for screening non-photosynthetic high-efficiency carbon immobilization microorganism under aerobic conditions Pending CN102660460A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103834586A (en) * 2013-12-30 2014-06-04 同济大学 Anaerobic carbon sequestration microbe screening method

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101225369A (en) * 2007-12-12 2008-07-23 中国水产科学研究院黄海水产研究所 Bacillus MP-2 with high-yield marine microorganism esterase and marine microorganism esterase generated thereby
CN101280278A (en) * 2008-05-20 2008-10-08 云南大学 Separation method and application of algicadal bacteria
CN101298597A (en) * 2007-04-30 2008-11-05 中国科学院沈阳应用生态研究所 Sea bacillus subtilis and screening method thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101298597A (en) * 2007-04-30 2008-11-05 中国科学院沈阳应用生态研究所 Sea bacillus subtilis and screening method thereof
CN101225369A (en) * 2007-12-12 2008-07-23 中国水产科学研究院黄海水产研究所 Bacillus MP-2 with high-yield marine microorganism esterase and marine microorganism esterase generated thereby
CN101280278A (en) * 2008-05-20 2008-10-08 云南大学 Separation method and application of algicadal bacteria

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
《环境科学学报》 20110630 武满满等 混合电子供体对好氧非光合微生物菌群固碳效率影响的析因实验分析 第1220-1226页 1-10 第31卷, 第6期 *
武满满等: "混合电子供体对好氧非光合微生物菌群固碳效率影响的析因实验分析", 《环境科学学报》, vol. 31, no. 6, 30 June 2011 (2011-06-30), pages 1220 - 1226 *
胡佳俊等: "非光合CO2 同化微生物菌群的选育/优化及其群落结构分析", 《环境科学》, vol. 30, no. 8, 31 August 2009 (2009-08-31), pages 2438 - 2444 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103834586A (en) * 2013-12-30 2014-06-04 同济大学 Anaerobic carbon sequestration microbe screening method

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Application publication date: 20120912