CN102660385B - Microwave extraction method for dryopteris fragrans(L.)schott essential oil - Google Patents
Microwave extraction method for dryopteris fragrans(L.)schott essential oil Download PDFInfo
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Abstract
The invention discloses a microwave extraction method for dryopteris fragrans(L.)schott essential oil. The optimum microwave extraction process is selected by controlling the sizes and water content of particles of dryopteris fragrans(L.)schott powder, the microwave intensity is set to be 100 to 500W, the extraction time is 10 to 50 minutes, and the high-quality dryopteris fragrans(L.)schott essential oil can be obtained. The method is easy to operate, and low in production cost; and the prepared product is high in activity and yield. The dryopteris fragrans(L.)schott essential oil prepared by the method has golden yellow color; and cosmetics which are prepared from extractive have the effect of resisting corynebaterium acnes and an obvious anti-aging effect.
Description
Technical field
The present invention relates to a kind of fragrant shield-fern extracts essential oil technology, especially a kind of microwave extract method.
Background technology
Fragrant shield-fern [Dryopteris fragrans (L.) Schott] is the Dryopteridaceae Dryopteris, it is the rare wild resources of medicinal plant of a kind of great exploitation potential for its and application prospect, refractory skins such as psoriasis, adolescent acne are had good therapeutic action, be regarded as " dermopathic jinx ".Pharmacological research finds that fragrant shield-fern has effect such as anti-oxidant, antibacterial preferably in recent years.
Essential oil is the class important biological material in the fragrant shield-fern, because fragrant shield-fern essential oil has anti-oxidant, bacteriostatic activity preferably, can be developed and is developed to corresponding medicine or is applied to industries such as makeup and food as additive.Yet still there is not fragrant shield-fern extracts essential oil method preferably at present, therefore the present invention is optimized with regard to the technology of the Different Extraction Method of fragrant shield-fern essential oil, thereby obtain more perfect fragrant shield-fern extracts essential oil method, for suitability for industrialized production and the follow-up research of fragrant shield-fern essential oil provides strong technical support.
Summary of the invention
Technical problem to be solved by this invention provides a kind of fragrant shield-fern essential oil and microwave extraction preparation technology thereof.
Technology provided by the present invention may further comprise the steps:
1) preparation sample: with the fragrant shield-fern over-ground part of fresh harvesting, after preliminary screening is removed impurity, dry in the shade naturally, the fragrant shield-fern over-ground part of drying is pulverized, the control granular size is the 20-80 order.
2) in fragrant shield-fern powder, add deionization, make fragrant shield-fern moisture content at 30%-70%, soak 0.5-1.5h
3) mixed solution being poured in the awl, awl is placed Microwave Extraction Equipment, is 100-500W by microwave power.
4) fragrant shield-fern volatile oil extraction time is 10-50min.
5) the essential oil anhydrous sodium sulfate drying of Ti Quing.
6) again by after the filter plate filtration, 80 ℃ of following 0.6% gacs stir decolouring 10-30min.
7) filter plate refilters and obtains fragrant shield-fern essential oil.
In order to improve fragrant shield-fern volatile oil productive rate, cross 40 mesh sieves after fragrant shield-fern is pulverized and be advisable; The water content of fragrant shield-fern is advisable at 55%-65%; Microwave extraction power is advisable at 200-400W; Fragrant shield-fern volatile oil extraction time 20-40min is advisable, wherein preferred 20min.
The inventive method is simple to operate, and production cost is low, and the product of preparation has higher activity, the productive rate height.The fragrant shield-fern volatile oil color of this method preparation is golden yellow; The anti-acne bacillus effect that the cosmetic product that adopts this extract to prepare has, other has remarkable anti-ageing effect.
Description of drawings
The fragrant shield-fern volatile oil of Fig. 1 different concns is to the fungistatic effect of Propiobacterium
1 is 2%; 2 is 5%, 3 to be 8; 4 is 10%, 5 to be 15%
The fragrant shield-fern volatile oil of Fig. 2 different concns is to the clearance rate of superoxide anion
Embodiment
Method among the following embodiment if no special instructions, is ordinary method.
Embodiment 1: the preparation of essential oil
1) preparation sample: with the fragrant shield-fern over-ground part of fresh harvesting, after preliminary screening is removed impurity, dry in the shade naturally, the fragrant shield-fern over-ground part of drying is pulverized (disintegrating apparatus: multifunctional crusher GX-04, Gao Xiang, Shanghai food machinery factory), the control granular size is 40 orders.
2) in fragrant shield-fern powder, add deionization, make fragrant shield-fern moisture content 55%, soak 0.5-1.5h
3) mixed solution is poured in the awl, awl is placed Microwave Extraction Equipment.In (pz-tq1 Microwave Extraction Equipment, Shanghai Pang Zhe microwave equipment company limited), be 200W by microwave power.
4) fragrant shield-fern volatile oil extraction time is 20min.
5) the essential oil anhydrous sodium sulfate drying of Ti Quing.
6) again by after the filter plate filtration, 80 ℃ of following 0.6% gacs stir decolouring 30min.
7) filter plate refilters and obtains fragrant shield-fern essential oil.
Embodiment 2: the preparation of essential oil
1) preparation sample: with the fragrant shield-fern over-ground part of fresh harvesting, after preliminary screening is removed impurity, dry in the shade naturally, the fragrant shield-fern over-ground part of drying is pulverized (disintegrating apparatus: multifunctional crusher GX-04, Gao Xiang, Shanghai food machinery factory), the control granular size is 40 orders.
2) in fragrant shield-fern powder, add deionization, make fragrant shield-fern moisture content 65%, soak 0.5-1.5h
3) mixed solution is poured in the awl, awl is placed Microwave Extraction Equipment.In (pz-tq1 Microwave Extraction Equipment, Shanghai Pang Zhe microwave equipment company limited), be 400W by microwave power.
4) fragrant shield-fern volatile oil extraction time is 40min.
5) the essential oil anhydrous sodium sulfate drying of Ti Quing.
6) again by after the filter plate filtration, 80 ℃ of following 0.6% gacs stir decolouring 30min.
7) filter plate refilters and obtains fragrant shield-fern essential oil.
Embodiment 3: the preparation of essential oil
1) preparation sample: with the fragrant shield-fern over-ground part of fresh harvesting, after preliminary screening is removed impurity, dry in the shade naturally, the fragrant shield-fern over-ground part of drying is pulverized (disintegrating apparatus: multifunctional crusher GX-04, Gao Xiang, Shanghai food machinery factory), the control granular size is the 20-80 order.
2) in fragrant shield-fern powder, add deionization, make fragrant shield-fern moisture content at 30%-70%, soak 0.5-1.5h
3) mixed solution is poured in the awl, awl is placed Microwave Extraction Equipment.In (pz-tq1 Microwave Extraction Equipment, Shanghai Pang Zhe microwave equipment company limited), be 100-500W by microwave power.
4) fragrant shield-fern volatile oil extraction time is 10-50min.
5) the essential oil anhydrous sodium sulfate drying of Ti Quing.
6) again by after the filter plate filtration, 80 ℃ of following 0.6% gacs stir decolouring 30min.
7) filter plate refilters and obtains fragrant shield-fern essential oil.
Embodiment 4: the inhibition Propiobacterium experiment of fragrant shield-fern volatile oil
The essential oil of embodiment 1-3 preparation is used for following test, and the result is similar.For the refining of summary of the invention, only provide the test-results of embodiment 2 essential oils at this.
(1) fungistatic effect of the antipruritic renovation agent stoste of plants antimicrobial
Propiobacterium is a kind of bacterium that the human body skin epidermis grows, and is the main germ of causing comedo, acne, acne.
(2) the Propiobacterium fungistatic effect of the fragrant shield-fern volatile oil of different concns
As seen from Figure 1, sample all has fungistatic effect to Propiobacterium under the situation of different concns, and the more high fungistatic effect of concentration is more good.
Embodiment 3: the anti-quick reparation experiment of the antipruritic renovation agent of plants antimicrobial
The essential oil of embodiment 1-3 preparation is used for following test, and the result is similar.For the refining of summary of the invention, only provide the test-results of embodiment 2 essential oils at this.
In organism, the decline of n cell antioxidant ability or the increase of active oxygen have caused oxidative stress.In human body, unbalance because the excess of free radical produces when oxygenant and the antioxidant of balance, will cause oxidative stress and dna damage.Superfluous free radical can be attacked DNA, causes matrix sudden change, singly-bound and double-strand break, DNA interlinkage, rhexis and gene rearrangement, finally causes human epidermal elasticity to reduce, and water retention reduces, and produces old and feeble performance.Superoxide anion is a kind of free radical of the present anti-ageing effect of a kind of verification sample relatively, the antidotal power of how much having reacted this sample of the clearance rate of superoxide anion.
Concrete grammar is: the Tris-HCl damping fluid 4.5ml that gets 0.05mol/L pH 8.2, place 25 ℃ of water-bath preheating 20min, add the fragrant shield-fern volatile oil of above-described embodiment 1 preparation of 1ml different concns and the pyrogallol solution of 0.4ml 25mmol/L respectively, in 25 ℃ of water-baths, react 5min behind the mixing, the HCl termination reaction that adds 1.0ml 8mol/L, make reference with the Tris-HCl damping fluid, measure absorbancy at the 299nm place, calculate clearance rate.The blank group replaces sample with the 1ml deionized water, and each is handled and all does three repetitions.
The calculation formula of clearance rate: ultra-oxygen anion free radical clearance rate (%)=100 (A1-A2)/A1, wherein A1 is the mean light absorbency of blank, A2 is the mean light absorbency of the lyophilized powder aqueous solution.
The fragrant shield-fern of the present invention preparation to the scavenging(action) result of ultra-oxygen anion free radical as shown in Figure 2.The result shows that fragrant shield-fern volatile oil concentration is at 40% o'clock, and the clearance rate of ultra-oxygen anion free radical is 49.2%, illustrates that fragrant shield-fern volatile oil has the effect that delays senility.
Claims (6)
1. the microwave extract method of a fragrant shield-fern essential oil is characterized in that comprising the steps:
1) preparation sample: with the fragrant shield-fern over-ground part of fresh harvesting, after preliminary screening is removed impurity, dry in the shade naturally, the fragrant shield-fern over-ground part of drying is pulverized, granular size is controlled at the 20-80 order;
2) in the fragrant shield-fern powder of step 1) preparation, add deionized water, make fragrant shield-fern moisture content at 30%-70%, soak 0.5-1.5h;
3) mixed solution is poured in the awl, awl is placed Microwave Extraction Equipment, the control microwave power is 100-500W; Extract 10-50min;
4) the essential oil anhydrous sodium sulfate drying of Ti Quing;
5) after the essential oil that obtains of step 4) filters by filter plate, 80 ℃ down 0.6% gac stir decolouring 30min;
6) essential oil of step 5) preparation filters by filter plate again and obtains fragrant shield-fern essential oil.
2. method according to claim 1, it is characterized in that: described granular size is 40 orders.
3. method according to claim 1, it is characterized in that: described step 2), the control water content is 55%-65%.
4. according to the arbitrary described method of claim 1-3, it is characterized in that described microwave power is 200-400W.
5. according to the arbitrary described method of claim 1-3, it is characterized in that: in the described step 3), extraction time is 20min.
6. method according to claim 1 is characterized in that the fragrant shield-fern essential oil that extracts is applied to antibacterial and/or anti-ageing field.
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| CN103897806B (en) * | 2014-04-03 | 2015-11-04 | 广东添乐化妆品有限公司 | A kind of microwave auxiliary extracting method of lemon quintessence oil |
| CN106281700A (en) * | 2016-08-16 | 2017-01-04 | 重庆雅香美源生态农业科技有限公司 | The extraction of Flos Rosae Rugosae quintessence oil and processing method |
| CN106281650A (en) * | 2016-08-16 | 2017-01-04 | 重庆雅香美源生态农业科技有限公司 | The extraction of a kind of five-leaved chaste tree quintessence oil and processing method |
| CN106281699A (en) * | 2016-08-16 | 2017-01-04 | 重庆雅香美源生态农业科技有限公司 | The extraction of a kind of Flos Tagetis Erectae quintessence oil and processing method |
| CN106244325A (en) * | 2016-08-16 | 2016-12-21 | 重庆雅香美源生态农业科技有限公司 | The extraction of a kind of Herba Acroptili repentis quintessence oil and processing method |
| CN107049866B (en) * | 2017-04-28 | 2020-05-08 | 东北农业大学 | Antiallergic plant composition and application thereof |
| CN107513470A (en) * | 2017-09-01 | 2017-12-26 | 北京国康本草物种生物科学技术研究院有限公司 | Plants essential oil and its extracting method and application |
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| CN101993773A (en) * | 2009-08-19 | 2011-03-30 | 新疆大学 | Method for extracting apricot kernel oil through microwave radiation |
| CN101649245A (en) * | 2009-09-14 | 2010-02-17 | 太仓文华实业有限公司 | Method for extracting peppermint oil by microwave radiation |
| CN101831350A (en) * | 2010-05-27 | 2010-09-15 | 胡保军 | Method for refining hazelnut oil |
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