CN102659915A - Method for preparing corn protein concentrates through enzymatic hydrolysis of com gluten meal - Google Patents
Method for preparing corn protein concentrates through enzymatic hydrolysis of com gluten meal Download PDFInfo
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- CN102659915A CN102659915A CN2012101296615A CN201210129661A CN102659915A CN 102659915 A CN102659915 A CN 102659915A CN 2012101296615 A CN2012101296615 A CN 2012101296615A CN 201210129661 A CN201210129661 A CN 201210129661A CN 102659915 A CN102659915 A CN 102659915A
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Abstract
The invention discloses a method for preparing corn protein concentrates through enzymatic hydrolysis of com gluten meal. The method adopts double-enzyme enzymolysis, namely that cellulose and alpha-amylase are respectively adopted to perform enzymolysis of the com gluten meal, and then centrifugal washing is performed to obtain the corn protein concentrates. When the com gluten meal is processed to prepare the corn protein concentrates, the cellulose and the alpha-amylase are respectively adopted to perform the enzymolysis, and polysaccharide, starch, cellulose and other macromolecules in the com gluten meal are all effectively degraded so that prepared corn protein concentrates are high in purity and utilization value and wide in application. No chemical reagent is adopted in the production process, reaction conditions are easily controlled, and the industrial production is easy to achieve.
Description
Technical field
The present invention relates to maize yellow-powder deep process technology field, relate in particular to the method that a kind of pair of enzyme enzymolysis maize yellow-powder prepares the corn protein concentrate.
Background technology
Maize yellow-powder is a kind of by product in the corn deep processing process; Specifically be meant a kind of tankage after wet method is produced W-Gum; Mainly contain corn protein,alcohol-soluble, gluten, a spot of starch, fat, fiber, and sphaeroprotein, BSA and the carrotenoid of trace.Because maize yellow-powder has special smell and color and luster, and corn protein,alcohol-soluble wherein can not be soluble in water, limited maize yellow-powder in Industrial Application, mainly be to be used for feedstuff industry or discharging naturally at present.
Further method for processing about maize yellow-powder has the extraction natural food colour at present; Protein,alcohol-soluble and L-glutamic acid etc. can also enzymolysis prepare the corn bioactive peptide of different physiological roles, like glutamine peptide, high F value oligopeptide, blood pressure lowering peptide, corn protein concentrate or corn protein peptide etc.But the product that aforesaid method is produced is too single-minded, and is limited for proteinic utilization ratio in the maize yellow-powder.Extract natural pigment and L-glutamic acid, product production is few; Can only be used as film after protein,alcohol-soluble extracts, this three's range of application is all limited.The corn bioactive peptide that enzymolysis obtains also can't be realized large-scale industrialization production at present because preparation is had relatively high expectations with purifying technique.Wherein, preparing in the technology of corn protein concentrate at enzymolysis, generally is after adopting neutral protease to carry out enzymolysis; Concentrate again and obtain the corn protein concentrate; Because degradeds such as the protein in the maize yellow-powder, polysaccharide, Mierocrystalline cellulose are not thorough, the product concentration that obtains is low, and utility value is not high.
Summary of the invention
Technical problem to be solved by this invention is: to the deficiency that prior art exists, provide a kind of maize yellow-powder good degrading effect, enzymatic hydrolysis maize yellow-powder that product purity is high to prepare the method for corn protein concentrate.
For solving the problems of the technologies described above, technical scheme of the present invention is:
A kind of enzymatic hydrolysis maize yellow-powder prepares the method for corn protein concentrate, adopts two enzyme enzymolysis, and said pair of enzyme enzymolysis is that centrifuge washing obtains the corn protein concentrate after adopting cellulase and AMS to carry out enzymolysis respectively said maize yellow-powder.
Wherein, said pair of enzyme enzymolysis may further comprise the steps:
(1) gets pulverizing, maize yellow-powder 100 weight parts after sieving; The water dissolution that adds 250～350 weight parts stirs, and heating in water bath and to keep temperature be 45～55 ℃ adds the cellulase of 0.5～1.5 weight part; Regulating the pH value is 4.5～5.5; Stirring reaction 2.5～3.5h, with the solution that obtains at the centrifugal 5～15min of 6000～8000r/min, then with solid substance water washed twice;
(2) above-mentioned solid substance is dissolved in the water of 250～350 weight parts; Heating in water bath and to keep temperature be 60～70 ℃ adds the AMS of 0.5～1.5 weight part, and regulating the pH value is 6.0～7.0; Stirring reaction 0.5～1.5h; The solution that obtains at the centrifugal 5～15min of 6000～8000r/min, with the centrifugal solid substance water washed twice that obtains, is obtained the corn protein concentrate.
When preferably, said maize yellow-powder sieves 60 mesh sieves.
Wherein, the employed acid of said adjusting pH value is the one or any several kinds combination in Hydrocerol A, oxysuccinic acid, acetate, sulfuric acid, hydrochloric acid, nitric acid and the phosphoric acid.
The alkali that said adjusting pH value is used is one or several the arbitrary combination in sodium hydroxide, Pottasium Hydroxide, calcium hydroxide, yellow soda ash, salt of wormwood, potassiumphosphate and the sodium phosphate.
Preferably, addition the best of said cellulase and AMS is 1 weight part.
Preferably, under the mixing speed of 800～1000r/min, carry out stirring reaction behind said adding cellulase and the AMS.
Said adding cellulase can exchange with the sequencing that the adding AMS carries out enzymolysis respectively.
As a kind of improvement, said employing cellulase and AMS carry out behind the enzymolysis centrifugal centrifugate of coming out and recycle the carbon source in fermention medium.
Owing to adopted technique scheme, the invention has the beneficial effects as follows:
The present invention is when using maize yellow-powder processing and preparing corn protein concentrate; Adopt cellulase and AMS to carry out enzymolysis respectively; Macromole such as the polysaccharide in the maize yellow-powder, starch, Mierocrystalline cellulose are all effectively degraded, and therefore the corn protein concentrate purity of preparation is high, and the mass content of corn protein concentrate is more than 70%; Utility value is high, and purposes is wide.And do not adopt any chemical reagent in the production process, easy control of reaction conditions is easy to realize suitability for industrialized production.
The present invention adopts cellulase and AMS to carry out behind the enzymolysis in the centrifugal centrifugate of coming out, and because of containing a certain amount of reducing sugar, the recyclable carbon source that is used in fermention medium has no discharge of wastewater to environment, the production technique environmental protection.
Embodiment
Below in conjunction with concrete embodiment, further set forth the present invention.Should be understood that these embodiment only to be used to the present invention is described and be not used in the restriction scope of the present invention.Should be understood that in addition those skilled in the art can do various changes or modification to the present invention after the content of having read the present invention's instruction, these equivalent form of values fall within the application's appended claims institute restricted portion equally.
Embodiment 1
(1) maize yellow-powder is pulverized with high speed disintegrator, crossed 60 mesh sieves.
(2) get pulverizing, the maize yellow-powder 100g after sieving, the water dissolution that adds 250g stirs, heating in water bath and to keep temperature be 45 ℃; The cellulase that adds 0.8g; Using the lemon acid for adjusting pH value is 4.5, at 800r/min stirring reaction 3.5h, with the solution that obtains at the centrifugal 15min of 6000r/min; The centrifugal centrifugate that obtains is placed separately, then with solid substance water washed twice.
(3) above-mentioned solid substance is dissolved in the water of 250g, heating in water bath and to keep temperature be 60 ℃ adds the AMS of 0.8g; Using sodium hydroxide to regulate the pH value is 6.0; At 800r/min stirring reaction 1.5h, at the centrifugal 15min of 6000r/min, the centrifugal centrifugate that obtains is placed separately with the solution that obtains; With the centrifugal solid substance water washed twice that obtains, obtain the corn protein concentrate.
Embodiment 2
(1) maize yellow-powder is pulverized with high speed disintegrator, crossed 60 mesh sieves.
(2) get pulverizing, the maize yellow-powder 100g after sieving, the water dissolution that adds 350g stirs, heating in water bath and to keep temperature be 55 ℃; The cellulase that adds 1.5g; Regulating the pH value is 5.5, stirring reaction 3.5h, with the solution that obtains at the centrifugal 5min of 8000r/min; The centrifugal centrifugate that obtains is placed separately, then with solid substance water washed twice.
(3) above-mentioned solid substance is dissolved in the water of 350g, heating in water bath and to keep temperature be 70 ℃ adds the AMS of 1.5g; Regulating the pH value is 7.0; Stirring reaction 1.5h, at the centrifugal 5min of 8000r/min, the centrifugal centrifugate that obtains is placed separately with the solution that obtains; With the centrifugal solid substance water washed twice that obtains, obtain the corn protein concentrate.
Embodiment 3
(1) maize yellow-powder is pulverized with high speed disintegrator, crossed 60 mesh sieves.
(2) get pulverizing, the maize yellow-powder 100g after sieving, the water dissolution that adds 300g stirs, heating in water bath and to keep temperature be 65 ℃; The AMS that adds 1.0g; Regulating the pH value is 6.5, at 900r/min stirring reaction 1.0h, with the solution that obtains at the centrifugal 10min of 7000r/min; The centrifugal centrifugate that obtains is placed separately, then with solid substance water washed twice.
(3) above-mentioned solid substance is dissolved in the water of 300g, heating in water bath and to keep temperature be 50 ℃ adds the cellulase of 1.0g; Regulating the pH value is 5.0; At 900r/min stirring reaction 3.0h, at the centrifugal 10min of 7000r/min, the centrifugal centrifugate that obtains is placed separately with the solution that obtains; With the centrifugal solid substance water washed twice that obtains, obtain the corn protein concentrate.
Embodiment 4
(1) maize yellow-powder is pulverized with high speed disintegrator, crossed 60 mesh sieves.
(2) get pulverizing, the maize yellow-powder 100g after sieving, the water dissolution that adds 290g stirs, heating in water bath and to keep temperature be 48 ℃; The cellulase that adds 1.2g; Using the salt acid for adjusting pH value is 4.8, at 1000r/min stirring reaction 2.8h, with the solution that obtains at the centrifugal 12min of 7500r/min; The centrifugal centrifugate that obtains is placed separately, then with solid substance water washed twice.
(3) above-mentioned solid substance is dissolved in the water of 280g, heating in water bath and to keep temperature be 68 ℃ adds the AMS of 1.2g; Using yellow soda ash to regulate the pH value is 6.2; At 9500r/min stirring reaction 1.2h, at the centrifugal 12min of 7500r/min, the centrifugal centrifugate that obtains is placed separately with the solution that obtains; With the centrifugal solid substance water washed twice that obtains, obtain the corn protein concentrate.
Claims (9)
1. an enzymatic hydrolysis maize yellow-powder prepares the method for corn protein concentrate; It is characterized in that: adopt two enzyme enzymolysis; Said pair of enzyme enzymolysis is that centrifuge washing obtains the corn protein concentrate after adopting cellulase and AMS to carry out enzymolysis respectively said maize yellow-powder.
2. enzymatic hydrolysis maize yellow-powder as claimed in claim 1 prepares the method for corn protein concentrate, it is characterized in that said pair of enzyme enzymolysis may further comprise the steps:
(1) gets pulverizing, maize yellow-powder 100 weight parts after sieving; The water dissolution that adds 250～350 weight parts stirs, and heating and maintenance temperature are 45～55 ℃, add the cellulase of 0.5～1.5 weight part; Regulating the pH value is 4.5～5.5; Stirring reaction 2.5～3.5h is with the solution centrifugal 5～15min that obtains, then with solid substance water washed twice;
(2) above-mentioned solid substance is dissolved in the water of 250～350 weight parts; Heating in water bath and to keep temperature be 60～70 ℃ adds the AMS of 0.5～1.5 weight part, and regulating the pH value is 6.0～7.0; Stirring reaction 0.5～1.5h; With the solution centrifugal 5～15min that obtains,, obtain the corn protein concentrate with the centrifugal solid substance water washed twice that obtains.
3. enzymatic hydrolysis maize yellow-powder as claimed in claim 2 prepares the method for corn protein concentrate, it is characterized in that: said maize yellow-powder was 60 mesh sieves when sieving.
4. enzymatic hydrolysis maize yellow-powder as claimed in claim 2 prepares the method for corn protein concentrate, it is characterized in that: the employed acid of said adjusting pH value is the one or any several kinds combination in Hydrocerol A, oxysuccinic acid, acetate, sulfuric acid, hydrochloric acid, nitric acid and the phosphoric acid.
5. enzymatic hydrolysis maize yellow-powder as claimed in claim 2 prepares the method for corn protein concentrate, it is characterized in that: the alkali that said adjusting pH value is used is one or several the arbitrary combination in sodium hydroxide, Pottasium Hydroxide, calcium hydroxide, yellow soda ash, salt of wormwood, potassiumphosphate and the sodium phosphate.
6. enzymatic hydrolysis maize yellow-powder as claimed in claim 2 prepares the method for corn protein concentrate, it is characterized in that: addition the best of said cellulase and AMS is 1 weight part.
7. enzymatic hydrolysis maize yellow-powder as claimed in claim 2 prepares the method for corn protein concentrate, it is characterized in that: under the mixing speed of 800～1000r/min, carry out stirring reaction behind said adding cellulase and the AMS respectively.
8. prepare the method for corn protein concentrate like described any enzymatic hydrolysis maize yellow-powder of claim 1 to 7, it is characterized in that: said adding cellulase can exchange with the sequencing that the adding AMS carries out enzymolysis respectively.
9. enzymatic hydrolysis maize yellow-powder as claimed in claim 8 prepares the method for corn protein concentrate, it is characterized in that: said employing cellulase and AMS carry out behind the enzymolysis centrifugal centrifugate of coming out and recycle.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2012101296615A CN102659915A (en) | 2012-04-27 | 2012-04-27 | Method for preparing corn protein concentrates through enzymatic hydrolysis of com gluten meal |
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| CN2012101296615A CN102659915A (en) | 2012-04-27 | 2012-04-27 | Method for preparing corn protein concentrates through enzymatic hydrolysis of com gluten meal |
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| CN102659915A true CN102659915A (en) | 2012-09-12 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108157675A (en) * | 2017-12-18 | 2018-06-15 | 陈建峰 | A kind of crab feed and preparation method thereof |
Citations (3)
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|---|---|---|---|---|
| CN101530153A (en) * | 2009-04-22 | 2009-09-16 | 湖南农业大学 | Method by adopting complex enzyme to prepare rice residue protein |
| CN101843259A (en) * | 2010-06-09 | 2010-09-29 | 天津实发中科百奥工业生物技术有限公司 | Process for preparing natural herbicide by using corn protein powder |
| CN102630801A (en) * | 2012-04-27 | 2012-08-15 | 山东盛泰生物科技有限公司 | Method for preparing corn protein foaming powder by enzymatic hydrolysis of com gluten meal |
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- 2012-04-27 CN CN2012101296615A patent/CN102659915A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101530153A (en) * | 2009-04-22 | 2009-09-16 | 湖南农业大学 | Method by adopting complex enzyme to prepare rice residue protein |
| CN101843259A (en) * | 2010-06-09 | 2010-09-29 | 天津实发中科百奥工业生物技术有限公司 | Process for preparing natural herbicide by using corn protein powder |
| CN102630801A (en) * | 2012-04-27 | 2012-08-15 | 山东盛泰生物科技有限公司 | Method for preparing corn protein foaming powder by enzymatic hydrolysis of com gluten meal |
Non-Patent Citations (2)
| Title |
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| 张铁鹰 等: "酶在玉米-豆粕日粮中的应用研究进展", 《动物营养学报》 * |
| 徐艳艳 等: "提取条件对玉米黄粉中淀粉含量影响的研究", 《食品工业科技》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108157675A (en) * | 2017-12-18 | 2018-06-15 | 陈建峰 | A kind of crab feed and preparation method thereof |
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Application publication date: 20120912 |