CN102657238B - Attractant for Asias halodendri, and attraction method thereof - Google Patents
Attractant for Asias halodendri, and attraction method thereof Download PDFInfo
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- CN102657238B CN102657238B CN 201210099910 CN201210099910A CN102657238B CN 102657238 B CN102657238 B CN 102657238B CN 201210099910 CN201210099910 CN 201210099910 CN 201210099910 A CN201210099910 A CN 201210099910A CN 102657238 B CN102657238 B CN 102657238B
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Abstract
The invention discloses an attractant for Asias halodendri, and an attraction method thereof. The attractant is composed of 90% of 5mug/mul essential oil of Elaeagnus angustifolia with flowers, 3% of 0.5mug/mul cis-3-hexen-1-ol and 7% of 0.5mug/mul trans-2-hexenal. The attraction method of the attractant comprises the steps of attraction core making from a preparation material, trap making, field trapping and the like. The attractant for trapping Asias halodendri of the invention has the characteristics of nontoxicity, harmlessness, environmental protection, no pollution, no residual, safety to humans and animals, strong pertinence, no damages to natural enemies and beneficial insects, no causing of the drug resistance of pests, simple operation, and low trapping cost. The developed attractant of the invention can trap many adults in a short period of time to reduce the harm of the adults to Tetraena mongolica Maxim.
Description
30060011), Tianjin natural science fund (fund number: 09JCYBJC14500), the doctor of Tianjin Normal University fund (fund number: carry out under subsidy 52XB1003) the present invention is at state natural sciences fund (fund number:.
Technical field
The invention belongs to the plant protection technology field, relate to the method for preventing and treating of plant insect, the attractant of a kind of red-edge longicorn of saying so more specifically and lure method.
Background technology
Red-edge longicorn [
Asias halodendri(Pallas)] claim red edge inferior longicorn again, belong to coleoptera (Coleoptora), Cerambycidae (Cerambycidae), longicorn subfamily (Cerambycfna) in classification, have a very wide distribution, domestic area North gets Heilungkiang, the Inner Mongol, Jilin, Liaoning, the south strides across the Changjiang river, to Jiangxi, Hunan, Hubei, Guangxi, Guizhou, Sichuan etc. ground, to the east of Shandong, Jiangsu and Zhejiang Provinces one band, the west reaches ground such as Qinghai, Tibet, the middle part has also comprised Gansu, Ningxia, Shanxi, Shaanxi, Hebei; It is domestic to be distributed in Korea, the former Soviet Union (Siberia, coastal area, the Far East) and Mongolia abroad.
Red-edge longicorn is a kind of borer pest, and its larva is food by the part of feeding plant trunk heartwood, heavy damage the transporting of trees nutrient and moisture, make the trees withered death.The host plant of having reported comprises that sea-buckthorn, four is closed wood, jujube, arrow-leaved oleaster, wild jujube, leaflet elm, locust tree, dry land willow, elm, matrimony vine, Acer negundo, honeysuckle, apple, Lee, willow, caragana, oak, white oak, oil tea, adds poplar, pears, grape, peach, acid thorn etc.This worm is serious to the forest harm of growing weak, especially injured fruit tree after cutting locust tree, American elm and the peeling after doing is injured seriously, can cause whole strain plant withered when serious.Domestic in the Inner Mongol, the harm that red-edge longicorn closes wood to important commodity trees sea-buckthorn and the endangered plants four that exist only in western Erdos area is very serious.
Sea-buckthorn (
Hippophae rhamnoides) be Elaeangnaceae (Elaeagnceae) Hippophne (
Hippophael) plant, be a kind of wild barbed machaka, be one of main reproducting tree species of water and soil conservation.Red-edge longicorn is one of main trunk borer of sea-buckthorn, and is serious to the branch of the harm of sea-buckthorn.As far back as 1993, Fan Renjun, Dong Jinming etc. just studied generation and the control of Shanxi area sea-buckthorn red-edge longicorn; The gentle prince of Guo hundred section in 1998 also harm and the control to this area's red-edge longicorn report; 2008, Tian Runmin, Lu Qun etc. were to two kinds of trunk borers of harm sea-buckthorn---and the laterality life relation of red-edge longicorn and Holcocerus hippophaecolus Larvae has been carried out preliminary research
[12]Because the harm of red-edge longicorn causes the decline of large tracts of land sea-buckthorn woods growing way, percentage of fertile fruit descends, even withered dead, has had a strong impact on the development and use of sea buckthorn resources.
Four close wood (
Tetreana mongolicaMaxim.) zygophyllaceae (Zygophyllaceae) plant, branch is rich in grease, very easily the combustion, be good fuel, for a long time by the local resident as fuel wood, so the title of " oily bavin " is arranged.Four close wood distribution very limited; only in desert district, middle part, Asia a small amount of distribution is arranged; mainly be distributed in the Ordos Plateau northwestward; on the south the Ku Buqi desert; to the east of cloth of ulan and the desert; the piedmont belt of the Table Mountain to the north of the Ya Maleike desert extended to adjacent crow on a small quantity and reaches low monadnock district, mountain, and this area was listed in Nature Reserve---nature reserve, western Erdos in 1996.Since 2006, in closing wooden core protection zone, four of western Erdos finds that four close the serious harm that wood suffers trunk borers such as red-edge longicorn, it is increasingly serious therefore to close the protection work of wood to four.For this reason, more and more scholars is devoted to the improvement to trunk borers such as red-edge longicorns.
The method of preventing and treating about the general employing of red-edge longicorn:
(1) forestry control: strengthen integrated management and cultivation management to gardens, improve the insect resistance capacity of forest self, strengthen tree vigo(u)r, in time remove backlog, withered wood, suppressed tree, wind-falls blowdown, fired-wood, be stranded mountain wood and serious killed wood.
(2) chemical control: the red-edge longicorn adult circle in the air ability a little less than, can take adult stage tree crown spraying control.Carry out the tree crown spraying with 40% Folithion missible oil, 80% DDT EC or 1000 times of liquid of 40% omethoate emulsifiable solution in the adult stage, kill adult, killing rate is more than 80%; Larval phase trunk punching injection medicament.The red-edge longicorn larval phase, is the longest, endanger hidden, be subjected to natural enemy and environmental influence minimum, in having, selection inhales conductivity, stronger scattering and permeating ability and stronger medicament of tagging with stomach poison function, as 40% omethoate emulsifiable solution, 50% acephatemet missible oil or 50% parathion missible oil, be mixed with 5 times of liquid, it is better to carry out butt punching injection control efficiency in the larva initial stage of origination, and killing rate is more than 84%.
(3) biological control: utilize the natural enemy of red-edge longicorn to suppress the growth of its population quantity.Red-edge longicorn has two kinds of parasitic wasps under field conditions (factors), is respectively the cutter ichneumon wasp
XoridesSp. with the moth ichneumon wasp
SchreineriaSp..The ectoparasite honeybee of monomer that these two kinds of ichneumon wasps all are red-edge longicorns, its ovum phase and larval phase, spent at host larva body; Also can be by the swollen leg honeybee of artificial feeding Shi Fangguanshi in the roundheaded borer phase at woodland
Scleroderma guaniXiao et Wu becomes not encyrtid of honeybee or Liao Shi soap
Zaommoencyrtus liaoiNatural enemies such as Triapitzin are controlled red-edge longicorn harm.In addition, the red-edge longicorn larval phase, also be subjected to the white muscardine fungi parasitism, but parasitic rate is very low.
Summary of the invention
The invention discloses a kind of attractant of red-edge longicorn and lure method.Because common control longicorn method often adopts chemical agent control, artificial control and some forest culture and management measures.Normal flolimat, cypermethrin, dichlorvos, Kang Fuduo and the Le Siben etc. of using of chemical agent control, this method environmental pollution is serious, toxic action is strong, simultaneously can killed natural enemies and beneficial insect in the use, a large amount of uses can cause the drug-fast generation of longicorn to form vicious circle, and can produce a large amount of residual etc.The artificial control, this method is comparatively limited to, the cost height, waste time and energy and efficient not high.And the forest culture and management measure is periodically longer, can not be short-term inner control insect, and the red-edge longicorn emergence time concentrates, so adopt the method can miss the best period that lures collection, the measure that this method can be used as a long-term control longicorn continues.
For achieving the above object, the invention discloses following technology contents:
A kind of attractant of red-edge longicorn is characterized in that it has following one-tenth to be grouped into:
Lure constituent concentration scope optium concentration percentage by weight
Wounded in the battle arrow-leaved oleaster essential oil 0.5ug/ul-10 ug/ul 5ug/ul 90%
Suitable-blatter alcohol 0.05ug/ul-5 ug/ul 0.5ug/ul 3%
Instead-2-hexenoic aldehyde 0.05ug/ul-10 ug/ul 0.5ug/ul 7%.
Employing attractant disclosed by the invention is lured the method for red-edge longicorn, it is characterized in that being undertaken by following step:
1. the materials and methods that lures core to make: select for use the penicillin bottle for luring the core carrier, bore an aperture to slow down the lure rate of release in bottle cap central authorities, add agar in the bottle as sustained release agent, wherein the w/v of agar and water is 1:50; The lure 200ul injection for preparing is equipped with in the bottle of agar.
2. the materials and methods made of trapper: trapper all adopts the pure water bottle of 550 ml colorless and odorless, 1/4 place below bottleneck, carve in the symmetric position of body both sides and to open 2 square osculums, the osculum diameter is 1 cm, and thin slice is upwards dug, and becomes 90 degree with body, add the long-pending chlorophos dilution of 1/4 pure water bottle in the bottle, extension rate is pressed the 1:600 thin up, with thin wire with the off-the-shelf core vertical hanging that lures in trapper, for next step field lures collection ready.
3. the field lures collection: trapper is hung four with thin wire close on the wooden plant, each trapper spacing is 10 m, and hanging position is selected the well-ventilated place, and every group of lure arranges 10 repetitions; Each is organized lure and arranges with parallel mode in the Experimental Area, and line space is 10 m; First lure trapping phase is 21 d, and second batch of lure trapping phase is 11 d; According to evaporation of liquid situation in the trapper, add during each the inspection in good time.
The more detailed method of the present invention is as follows:
The field lures the collection experimental study to show that lure W effect is the most obvious, and its concrete composition is as follows:
| The lure composition | Each constituent concentration | Percentage |
| Colored arrow-leaved oleaster essential oil is arranged | 5ug/ul | 90% |
| Suitable-blatter alcohol | 0.5ug/ | 3% |
| Instead-the 2-hexenoic aldehyde | 0.5ug/ul | 7% |
The method of luring of attractant:
1. the materials and methods that lures core to make: select for use the penicillin bottle for luring the core carrier, bore an aperture to slow down the lure rate of release in bottle cap central authorities, add agar in the bottle as sustained release agent (agar and water ratio are 1:50). the lure for preparing is injected be equipped with in the bottle of agar.
2. the materials and methods made of trapper: trapper all adopts the pure water bottle of 550 ml colorless and odorless, 1/4 place below bottleneck, carve in the symmetric position of body both sides and to open 2 square osculums, the osculum diameter is 1 cm, thin slice is upwards dug, become 90 degree with body, help to prevent entering of rainwater, and be conducive to attractant to external diffusion, add the long-pending chlorophos dilution of 1/4 pure water bottle in the bottle, extension rate is pressed the 1:600 thin up, with thin wire with the off-the-shelf core vertical hanging that lures in trapper, for next step field lures collection ready.
3. the field lures collection: trapper is hung four with thin wire close on the wooden plant, each trapper spacing is 10 m, and hanging position is selected the well-ventilated place, and every group of lure arranges 10 repetitions.Each is organized lure and arranges with parallel mode in the Experimental Area, and line space is 10 m.First lure trapping phase is 21 d, and second batch of lure trapping phase is 11 d.According to evaporation of liquid situation in the trapper, add during each the inspection in good time.
Adopt the inspection of the actual trap effect of lure of the present invention:
From hanging from trapper, check one by one every day to lure the insect pest situation condition in each trapper, lure collection to insect all pull out, red-edge longicorn quantity and male and female sex are added up, and will lure collect to other kind insect take back and indoorly add up and identify.
Be the trap effect of 25 kinds of lures in the following table, the effect of lure W is best.The W lure is exactly this lure of applying for a patent.The attractant effect of different attractants:
(1) test method:
Trapping is to 87 of adults altogether in this field trapping experiment, and wherein female worm is 36, and 51 of male worms the results are shown in Table 1.If be measurement index with " relatively trappability ", second batch (attractant effect of each lure of T～Y) all significantly better than first (A～S), wherein the effect of lure W is best, and relative trappability reaches 21.84%, be significantly higher than other 24 kinds of lures (
P＜0.05), be lure T secondly, trappability reaches 17.24% relatively, is significantly higher than other 23 kinds of lures (P＜0.05).(trap effect of lure D is better in 19 kinds of lures of A～S) for first.
T, X lure proportioning:
(2) result of experiment:
25 kinds of attractants of table 1 lure the collection effect
Annotate: different significance level P＜0.05 that reaches of the different lowercase alphabet differentials that mark after the same column data in the table.
Result of experiment shows:
The red-edge longicorn adult of lure W lure the collection effect best, relatively trappability is higher than other 24 kinds of lures.
Attractant advantage disclosed by the invention is:
1. this lure material is plant volatiles, nonhazardous, environment friendly and pollution-free, noresidue, to the person poultry safety.
2. with strong points, do not injure natural enemy and beneficial insect, can not cause that insect develops immunity to drugs.
3. easy and simple to handle, the trapping cost is low, has reduced the amount of labour greatly.
4. efficient height.Because the red-edge longicorn emergence time concentrates, so be the best period that lures collection this period, at these characteristics of red-edge longicorn, the development of this attractant can lure collection to more adult within a short period of time, and then has reduced adult to four harm of closing wood.
5. longer duration.The normal rubber cap that adopts is as luring the core carrier in other researchs, and its volatilization is fast, and slow release effect is general.This lure adopts slow release method, and the lure volatilization is slow, and longer duration does not need often to add lure.
Description of drawings:
Fig. 1 is the structural representation of trapper; Wherein 1 is the core that lures of vertical hanging, and 2 is the thin slice of upwards digging; 3 is pure water bottle.
Embodiment
Below in conjunction with embodiment the present invention is described, the scheme of embodiment described here, do not limit the present invention, one of skill in the art can make improvements and change according to spirit of the present invention, described these improvement and variation all should be considered as within the scope of the invention, and scope of the present invention and essence are limited by claim.Of the present invention to have colored arrow-leaved oleaster, suitable-blatter alcohol, anti--2-hexenoic aldehyde to have commercially available, and other used reagent also have commercially available.
Embodiment 1
Lure composition optium concentration percentage by weight
Wounded in the battle arrow-leaved oleaster essential oil 5ug/ul 90%
Suitable-blatter alcohol 0.5ug/ul 3%
Instead-2-hexenoic aldehyde 0.5ug/ul 7%.
Lure the method for red-edge longicorn:
1. the materials and methods that lures core to make: select for use the penicillin bottle for luring the core carrier, bore an aperture to slow down the lure rate of release in bottle cap central authorities, add agar in the bottle as sustained release agent, wherein the w/v of agar and water is 1:50; The lure 200ul injection for preparing is equipped with in the bottle of agar.
2. the materials and methods made of trapper: trapper all adopts the pure water bottle of 550 ml colorless and odorless, 1/4 place below bottleneck, carve in the symmetric position of body both sides and to open 2 square osculums, the osculum diameter is 1 cm, and thin slice is upwards dug, and becomes 90 degree with body, add the long-pending chlorophos dilution of 1/4 pure water bottle in the bottle, extension rate is pressed the 1:600 thin up, with thin wire with the off-the-shelf core vertical hanging that lures in trapper, for next step field lures collection ready.
3. the field lures collection: trapper is hung four with thin wire close on the wooden plant, each trapper spacing is 10 m, and hanging position is selected the well-ventilated place, and every group of lure arranges 10 repetitions; Each is organized lure and arranges with parallel mode in the Experimental Area, and line space is 10 m; First lure trapping phase is 21 d, and second batch of lure trapping phase is 11 d; According to evaporation of liquid situation in the trapper, add during each the inspection in good time.
Embodiment 2
Each constituent concentration percentage by weight of lure composition
Wounded in the battle arrow-leaved oleaster essential oil 8ug/ul 90%
Suitable-blatter alcohol 0.8ug/ul 3%
Instead-2-hexenoic aldehyde 5ug/ul 7%.
Lure the method for red-edge longicorn:
1. the materials and methods that lures core to make: select for use the penicillin bottle for luring the core carrier, bore an aperture to slow down the lure rate of release in bottle cap central authorities, add agar in the bottle as sustained release agent, wherein the w/v of agar and water is 1:50; The lure 200ul injection for preparing is equipped with in the bottle of agar.
2. the materials and methods made of trapper: trapper all adopts the pure water bottle of 550 ml colorless and odorless, 1/4 place below bottleneck, carve in the symmetric position of body both sides and to open 2 square osculums, the osculum diameter is 1 cm, and thin slice is upwards dug, and becomes 90 degree with body, add the long-pending chlorophos dilution of 1/4 pure water bottle in the bottle, extension rate is pressed the 1:600 thin up, with thin wire with the ready core vertical hanging that lures in trapper, for next step field lures collection ready.
3. the field lures collection: trapper is hung four with thin wire close on the wooden plant, each trapper spacing is 10 m, and hanging position is selected the well-ventilated place, and every group of lure arranges 10 repetitions; Each is organized lure and arranges with parallel mode in the Experimental Area, and line space is 10 m; First lure trapping phase is 21 d, and second batch of lure trapping phase is 11 d; According to evaporation of liquid situation in the trapper, add during each the inspection in good time.
Lure constituent concentration scope percentage by weight
Wounded in the battle arrow-leaved oleaster essential oil 0.5ug/ul 90%
Suitable-blatter alcohol 0.05ug/ul 3%
Instead-2-hexenoic aldehyde 1 ug/ul 7%;
Lure the method for method example 1 work simultaneously of red-edge longicorn.
Embodiment 4
Lure constituent concentration scope percentage by weight
Wounded in the battle arrow-leaved oleaster essential oil 6ug/ul 90%
Suitable-blatter alcohol 0.8ug/ul 3%
Instead-2-hexenoic aldehyde 5 ug/ul 7%;
Lure the method for method example 1 work simultaneously of red-edge longicorn.
Embodiment 5
Lure constituent concentration scope percentage by weight
Wounded in the battle arrow-leaved oleaster essential oil 8ug/ul 90%
Suitable-blatter alcohol 0.5ug/ul 3%
Instead-2-hexenoic aldehyde 1.5 ug/ul 7%;
Lure the method for method example 1 work simultaneously of red-edge longicorn.
Embodiment 6
Lure constituent concentration scope percentage by weight
Wounded in the battle arrow-leaved oleaster essential oil 3ug/ul 90%
Suitable-blatter alcohol 0.5ug/ul 3%
Instead-2-hexenoic aldehyde 0.5 ug/ul 7%;
Lure the method for method example 1 work simultaneously of red-edge longicorn.
Embodiment 7
Lure constituent concentration scope percentage by weight
Wounded in the battle arrow-leaved oleaster essential oil 4ug/ul 90%
Suitable-blatter alcohol 0.05ug/ul 3%
Instead-2-hexenoic aldehyde 0.05 ug/ul 7%;
Lure the method for method example 1 work simultaneously of red-edge longicorn.
Embodiment 4
Best preferred determining of forming
Show that by above-mentioned experimental study colored arrow-leaved oleaster essential oil is arranged when concentration is 5 ug/ul, its EAG value is 2.83, is higher than the response value of other concentration on the same group, the behavior selection rate is 83.3%, also is higher than the behavior selection rate under other concentration on the same group; Suitable-when blatter alcohol concentration was 0.5ug/ul, its EAG value was 2.56, was higher than the response value of other concentration on the same group, behavior selection rate 63.3% also is higher than the behavior selection rate under other concentration on the same group; Instead-and when the 2-hexenoic aldehyde was 0.5ug/ul in concentration, effect was obvious, and its EAG value is 3.31, is higher than the response value of other concentration on the same group, and the behavior selection rate is 73.3%, also is higher than the selection rate under other concentration on the same group.The result shows have colored arrow-leaved oleaster essential oil, suitable-blatter alcohol and the anti--optium concentration of 2-hexenoic aldehyde in experiment to be respectively 5 ug/ul, 0.5ug/ul and 0.5ug/ul.
Claims (2)
1. the attractant of a red-edge longicorn is characterized in that it is to be grouped into by following one-tenth:
Lure constituent concentration scope percentage by weight
Wounded in the battle arrow-leaved oleaster essential oil 0.5ug/ul-10 ug/ul 90%
Suitable-blatter alcohol 0.05ug/ul-5 ug/ul 3%
Instead-2-hexenoic aldehyde 0.05ug/ul-10 ug/ul 7%.
2. the described attractant of claim 1 is characterized in that it is to be grouped into by following one-tenth:
Lure constituent concentration percentage by weight
Wounded in the battle arrow-leaved oleaster essential oil 5ug/ul 90%
Suitable-blatter alcohol 0.5ug/ul 3%
Instead-2-hexenoic aldehyde 0.5ug/ul 7%.
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| CN 201210099910 CN102657238B (en) | 2012-04-09 | 2012-04-09 | Attractant for Asias halodendri, and attraction method thereof |
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| CN103004903B (en) * | 2012-12-21 | 2014-06-04 | 广东省昆虫研究所 | Phyllo treta striolata attractant |
| IL230774A0 (en) * | 2014-02-02 | 2014-09-30 | Westham Ltd | Attractants and bait stations comprising date-derived syrup products for attracting flies and methods therein |
| KR101530827B1 (en) * | 2014-08-19 | 2015-06-24 | 박영식 | Control method of pine wood nematode by monitoring and mass-trapping of Monochamus aternatus and Monochamus saltuarius |
| CN106035285A (en) * | 2016-08-09 | 2016-10-26 | 程林 | Surrounding soil box type Niphades castanes Chao trapping device |
| CN107593619A (en) * | 2017-09-29 | 2018-01-19 | 天津拓华生物科技有限责任公司 | A kind of method of outdoor artificial feeding red-edge longicorn |
| CN107593618A (en) * | 2017-09-29 | 2018-01-19 | 天津拓华生物科技有限责任公司 | A kind of method of indoors artificial raising red-edge longicorn |
| CN108323532A (en) * | 2018-02-13 | 2018-07-27 | 四川省农业科学院植物保护研究所 | A kind of slow-release citrus fruit fly attractant and production method |
| CN109769867A (en) * | 2018-09-21 | 2019-05-21 | 北京中捷四方生物科技股份有限公司 | A kind of Emerald ash borer imago attractant lure and preparation method thereof |
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